LOC110989230 cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110989230
Entrez Gene ID	110989230
Full Name	methionine--tRNA ligase, cytoplasmic-like
Gene Type	protein-coding
Organism	Acanthaster planci(crown-of-thorns starfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022253462.1	XP_022109154.1	methionine--tRNA ligase, cytoplasmic-like isoform X1
XM_022253463.1	XP_022109155.1	methionine--tRNA ligase, cytoplasmic-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC110989230 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110989230 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc172411	XM_022253462.1 Latest version!	Acanthaster planci methionine--tRNA ligase, cytoplasmic-like (LOC110989230), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc172412	XM_022253463.1 Latest version!	Acanthaster planci methionine--tRNA ligase, cytoplasmic-like (LOC110989230), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110989230 ( XM_022253462.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022109154.1 Acanthaster planci methionine--tRNA ligase, cytoplasmic-like (LOC110989230), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc172411
Clone ID Related Accession (Same CDS sequence)	XM_022253462.1
Accession Version	XM_022253462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	methionine--tRNA ligase, cytoplasmic-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTAT TGACAGATAA AGGGAATCAG CACTGTCTTA AGGCTGTTAT TGCTGCATGT 
CTTGCAGGGG TTAAACTGGA CGTGGAAGAA GTTGAAAACA CGGATCATGT TGTGCCTTAC 
CTGAGGAAGA ATCGTTTGCC AGTTCTCAAT CTGGGCAATG GAGATTTCAT TTTTTCTGCA 
AACAGTATAT GCAGGTATCT GCTTGCCCAA GGTGCCTTGG ATGTTGATGA CACACAGGTG 
GATGAGTGGC TGGAGTGGGA GGCTACGCAA CTGCAGCCCG TGCTTGGATC GTTCTTGGCA 
TTGTTATACG GGAGTGGCAA AGCCAACGCC GACTGCTTGA AACCGTTGCT GACTCACCTC 
AACAGTTCCC TGCAGGGAAA CCGGTATCTG GTTGGGAATG GCTTATCAGT GGCAGATATC 
AGTGTCTGGT CAGCCTTGTA CCCAGCGCAG GCCACCAGGT CTGATCTCCT CCCCGCCTAC 
CCCCACGTGG AATCCTGGTT CAGGGGCCTC AGCGGGCAGA AGGCGTTTGA AGCCAGCGTT 
GCTGTGGTAA CCCACAGCCA GGGAGCCGGC AGCTTCAAGG CATCCATTTT GGCCCAGCCC 
ATTGTTCAGA TGCCCGTTGG GGATGCCAGG ACGGCAAAAG ACGAAGAGTC TGCCGACACC 
GAGCAGGACG TCACGAGTGA AGAGATTGAG CGTGCCAGGC AGGCTTGGTT GCTCGGTAAA 
GCCGACGCAC CGACGCCGAG GGCGAGAAAC CATCCGCTTC TTCCGGAGAA GGATCGGCGC 
AACGTCTTGA TCACCAGTGC CTTGCCTTAC GTCAACAACG TCCCCCACCT GGGCAATATC 
ATCGGTTGCG TGCTGAGCGC CGACGTGTTC TCAAGATTTT GTCGTTTGAG GAACTACAAC 
TGCCTGTACA TCTGCGGGAC CGACGAGTAC GGGACGGCCA CAGAAACCAA GGCGTTGGCC 
GAGGGGCTCA CCCCCCAGCA AATCTGCGAT AAGTACTACC GCATCCACTA TGACGTCTAC 
ACCTGGTTCA GCATCGACTT TGATTATTTT GGGAGGACGA CAACCAAACA GCAAACGGAG 
ATTGCCCAGG ATATTTTTCT CCGTCTCCAC CAGCGCGGCT TCCTTCTCAA GGACACGGTG 
GAGCAGCTGC TGTGTGTCAA GTGCAATCGG TTCCTGGCTG ACCGCTTTGT GGAGGGCACC 
TGCCCCCTGT GCTACTATCC CGACGCCAGG GGGGACCAGT GCGACAAGTG TGGCAAACTC 
ATCAATGCCA CAGAGCTCAA GAAACCCCGC TGCAAAGTCT GCAAGAAGTC CCCCATCATC 
AAGAACTCGG AGCACCTCTT CCTGGACCTG CCCAAGCTGC AGCCCCAGGT TCAGGCCTAC 
TTCGAGCGGG TGGCAGCAGC GGGTGACTGG ACAGCCAATG CCAAGTACGT CACCTCCAGC 
TGGCTGCGGG ATGGCCTCAA GCCCCGCTGC ATCACCAGGG ACCTGAAGTG GGGCACCCCG 
GTGCCCCTGG ATGGCTTCCG GGATAAGGTG TTCTACGTCT GGTTTGACGC GCCCATCGGC 
TACCCGTCCA TCACGGCCAA CTACACGGAC CAGTGGGAGA GGTGGTGGAA AAACCCAGAG 
GAGGTGGAAC TGTACAACTT CATGGCCAAA GACAACATCC CATTCCACAG TGTGGTGTTT 
CCCTGCACGC TGATTGGAGC TGAGGACAAC TACACCATCG TCAATCACCT CATTGCAGCT 
GAATACCTGA ATTACGAAGA CGGCAAGTTC TCCAAGAGCC GGGGGATCGG CGTGTTTGGC 
GACATGGCCA AGGACACAGG CATCCCTAGT GACATCTGGC GCTTCTACCT GCTGTACGTA 
CGCCCAGAGA CCATGGACAG TACCTTCAGC TGGGCAGACC TCATGGTGAA GAATAACAGC 
GAACTGCTGA ATAATCTTGG TAACTTCATC AACAGGTCCC TGACATTCCT CGAGAAGAAT 
TTTGGCAGTG TCGTCCCCGA GATGACCCTG GAGGAGGACG ACGAGCGGTT GCTGGCACTG 
GTCACTCGCG AGTTGACCTC CTACAAGCAG CTGCTGGAGA AGTCCAAGAT CCGGGATGCC 
CTGCGCCACA TCCTCAACAT CTCCCGCCTG GGCAACCAGC ACATACAGGC CAATCAGCCC 
TGGGTGCTGG TCAAGGGTGG AGACAAGGAG AGAGCCAGAG CGGGCGGCGT GATTGGCCTG 
TGCACGAACA TCTCCTGCCT GGTATCCACC ATGCTGCTGC CCTACATGCC GTTAACCAGC 
CGCACCATCC AGGAGCAGCT CAACGCTCCG CCGGAATGCA ACGTCATCAC TGAGAAGTTT 
GTATGCTACC TGGAGACAGG CCACAAGATC GGGACGCCCA AGCCACTATT TGGCAAGTTA 
GAACCTTCCC TAATTGAGAG TCTCAAGAAG AAATTCTCCG GTCAGCAGAA ACAGGTTGGT 
GACTCTGGTG ACCCAAAAGC AGCACCAGTT AGTTCAGTGC CCGTTGACGG CCCAGCTGCA 
CCGGCTGGCG ATGCCAGTGA GGTCAAACGG CTGACCGATG AGGTTGCCAA GCAGGGAAAC 
ATCGTGCGCA AGCTGAAGTC AGAGAAGGCC GACAAATCAG AGATCGAGAA GGAGGTGAAG 
AAGCTGCTGG CCCTCAAGCA AGAGTTGGCA CTGACCTCGG GTCAGGACCC GACCCCTGTG 
GAATCCTCCA AGGGGAAGAG GAAAAAAGGA GGAAAGAAGA AAGAGGAAAA ATCAAAAAAC 
TCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022109154.1
CDS	122..2890
Translation	MKLLTDKGNQHCLKAVIAACLAGVKLDVEEVENTDHVVPYLRKNRLPVLNLGNGDFIFSANSICRYLLAQGALDVDDTQVDEWLEWEATQLQPVLGSFLALLYGSGKANADCLKPLLTHLNSSLQGNRYLVGNGLSVADISVWSALYPAQATRSDLLPAYPHVESWFRGLSGQKAFEASVAVVTHSQGAGSFKASILAQPIVQMPVGDARTAKDEESADTEQDVTSEEIERARQAWLLGKADAPTPRARNHPLLPEKDRRNVLITSALPYVNNVPHLGNIIGCVLSADVFSRFCRLRNYNCLYICGTDEYGTATETKALAEGLTPQQICDKYYRIHYDVYTWFSIDFDYFGRTTTKQQTEIAQDIFLRLHQRGFLLKDTVEQLLCVKCNRFLADRFVEGTCPLCYYPDARGDQCDKCGKLINATELKKPRCKVCKKSPIIKNSEHLFLDLPKLQPQVQAYFERVAAAGDWTANAKYVTSSWLRDGLKPRCITRDLKWGTPVPLDGFRDKVFYVWFDAPIGYPSITANYTDQWERWWKNPEEVELYNFMAKDNIPFHSVVFPCTLIGAEDNYTIVNHLIAAEYLNYEDGKFSKSRGIGVFGDMAKDTGIPSDIWRFYLLYVRPETMDSTFSWADLMVKNNSELLNNLGNFINRSLTFLEKNFGSVVPEMTLEEDDERLLALVTRELTSYKQLLEKSKIRDALRHILNISRLGNQHIQANQPWVLVKGGDKERARAGGVIGLCTNISCLVSTMLLPYMPLTSRTIQEQLNAPPECNVITEKFVCYLETGHKIGTPKPLFGKLEPSLIESLKKKFSGQQKQVGDSGDPKAAPVSSVPVDGPAAPAGDASEVKRLTDEVAKQGNIVRKLKSEKADKSEIEKEVKKLLALKQELALTSGQDPTPVESSKGKRKKGGKKKEEKSKNSE

Target ORF information:

RefSeq Version	XM_022253462.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci methionine--tRNA ligase, cytoplasmic-like (LOC110989230), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022253462.1

ORF Insert Sequence:

ATGAAACTAT TGACAGATAA AGGGAATCAG CACTGTCTTA AGGCTGTTAT TGCTGCATGT 
CTTGCAGGGG TTAAACTGGA CGTGGAAGAA GTTGAAAACA CGGATCATGT TGTGCCTTAC 
CTGAGGAAGA ATCGTTTGCC AGTTCTCAAT CTGGGCAATG GAGATTTCAT TTTTTCTGCA 
AACAGTATAT GCAGGTATCT GCTTGCCCAA GGTGCCTTGG ATGTTGATGA CACACAGGTG 
GATGAGTGGC TGGAGTGGGA GGCTACGCAA CTGCAGCCCG TGCTTGGATC GTTCTTGGCA 
TTGTTATACG GGAGTGGCAA AGCCAACGCC GACTGCTTGA AACCGTTGCT GACTCACCTC 
AACAGTTCCC TGCAGGGAAA CCGGTATCTG GTTGGGAATG GCTTATCAGT GGCAGATATC 
AGTGTCTGGT CAGCCTTGTA CCCAGCGCAG GCCACCAGGT CTGATCTCCT CCCCGCCTAC 
CCCCACGTGG AATCCTGGTT CAGGGGCCTC AGCGGGCAGA AGGCGTTTGA AGCCAGCGTT 
GCTGTGGTAA CCCACAGCCA GGGAGCCGGC AGCTTCAAGG CATCCATTTT GGCCCAGCCC 
ATTGTTCAGA TGCCCGTTGG GGATGCCAGG ACGGCAAAAG ACGAAGAGTC TGCCGACACC 
GAGCAGGACG TCACGAGTGA AGAGATTGAG CGTGCCAGGC AGGCTTGGTT GCTCGGTAAA 
GCCGACGCAC CGACGCCGAG GGCGAGAAAC CATCCGCTTC TTCCGGAGAA GGATCGGCGC 
AACGTCTTGA TCACCAGTGC CTTGCCTTAC GTCAACAACG TCCCCCACCT GGGCAATATC 
ATCGGTTGCG TGCTGAGCGC CGACGTGTTC TCAAGATTTT GTCGTTTGAG GAACTACAAC 
TGCCTGTACA TCTGCGGGAC CGACGAGTAC GGGACGGCCA CAGAAACCAA GGCGTTGGCC 
GAGGGGCTCA CCCCCCAGCA AATCTGCGAT AAGTACTACC GCATCCACTA TGACGTCTAC 
ACCTGGTTCA GCATCGACTT TGATTATTTT GGGAGGACGA CAACCAAACA GCAAACGGAG 
ATTGCCCAGG ATATTTTTCT CCGTCTCCAC CAGCGCGGCT TCCTTCTCAA GGACACGGTG 
GAGCAGCTGC TGTGTGTCAA GTGCAATCGG TTCCTGGCTG ACCGCTTTGT GGAGGGCACC 
TGCCCCCTGT GCTACTATCC CGACGCCAGG GGGGACCAGT GCGACAAGTG TGGCAAACTC 
ATCAATGCCA CAGAGCTCAA GAAACCCCGC TGCAAAGTCT GCAAGAAGTC CCCCATCATC 
AAGAACTCGG AGCACCTCTT CCTGGACCTG CCCAAGCTGC AGCCCCAGGT TCAGGCCTAC 
TTCGAGCGGG TGGCAGCAGC GGGTGACTGG ACAGCCAATG CCAAGTACGT CACCTCCAGC 
TGGCTGCGGG ATGGCCTCAA GCCCCGCTGC ATCACCAGGG ACCTGAAGTG GGGCACCCCG 
GTGCCCCTGG ATGGCTTCCG GGATAAGGTG TTCTACGTCT GGTTTGACGC GCCCATCGGC 
TACCCGTCCA TCACGGCCAA CTACACGGAC CAGTGGGAGA GGTGGTGGAA AAACCCAGAG 
GAGGTGGAAC TGTACAACTT CATGGCCAAA GACAACATCC CATTCCACAG TGTGGTGTTT 
CCCTGCACGC TGATTGGAGC TGAGGACAAC TACACCATCG TCAATCACCT CATTGCAGCT 
GAATACCTGA ATTACGAAGA CGGCAAGTTC TCCAAGAGCC GGGGGATCGG CGTGTTTGGC 
GACATGGCCA AGGACACAGG CATCCCTAGT GACATCTGGC GCTTCTACCT GCTGTACGTA 
CGCCCAGAGA CCATGGACAG TACCTTCAGC TGGGCAGACC TCATGGTGAA GAATAACAGC 
GAACTGCTGA ATAATCTTGG TAACTTCATC AACAGGTCCC TGACATTCCT CGAGAAGAAT 
TTTGGCAGTG TCGTCCCCGA GATGACCCTG GAGGAGGACG ACGAGCGGTT GCTGGCACTG 
GTCACTCGCG AGTTGACCTC CTACAAGCAG CTGCTGGAGA AGTCCAAGAT CCGGGATGCC 
CTGCGCCACA TCCTCAACAT CTCCCGCCTG GGCAACCAGC ACATACAGGC CAATCAGCCC 
TGGGTGCTGG TCAAGGGTGG AGACAAGGAG AGAGCCAGAG CGGGCGGCGT GATTGGCCTG 
TGCACGAACA TCTCCTGCCT GGTATCCACC ATGCTGCTGC CCTACATGCC GTTAACCAGC 
CGCACCATCC AGGAGCAGCT CAACGCTCCG CCGGAATGCA ACGTCATCAC TGAGAAGTTT 
GTATGCTACC TGGAGACAGG CCACAAGATC GGGACGCCCA AGCCACTATT TGGCAAGTTA 
GAACCTTCCC TAATTGAGAG TCTCAAGAAG AAATTCTCCG GTCAGCAGAA ACAGGTTGGT 
GACTCTGGTG ACCCAAAAGC AGCACCAGTT AGTTCAGTGC CCGTTGACGG CCCAGCTGCA 
CCGGCTGGCG ATGCCAGTGA GGTCAAACGG CTGACCGATG AGGTTGCCAA GCAGGGAAAC 
ATCGTGCGCA AGCTGAAGTC AGAGAAGGCC GACAAATCAG AGATCGAGAA GGAGGTGAAG 
AAGCTGCTGG CCCTCAAGCA AGAGTTGGCA CTGACCTCGG GTCAGGACCC GACCCCTGTG 
GAATCCTCCA AGGGGAAGAG GAAAAAAGGA GGAAAGAAGA AAGAGGAAAA ATCAAAAAAC 
TCTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110989230 ( XM_022253463.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022109155.1 Acanthaster planci methionine--tRNA ligase, cytoplasmic-like (LOC110989230), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc172412
Clone ID Related Accession (Same CDS sequence)	XM_022253463.1
Accession Version	XM_022253463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	methionine--tRNA ligase, cytoplasmic-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091632.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTAT TGACAGATAA AGGGAATCAG CACTGTCTTA AGGCTGTTAT TGCTGCATGT 
CTTGCAGGGG TTAAACTGGA CGTGGAAGAA GTTGAAAACA CGGATCATGT TGTGCCTTAC 
CTGAGGAAGA ATCGTTTGCC AGTTCTCAAT CTGGGCAATG GAGATTTCAT TTTTTCTGCA 
AACAGTATAT GCAGGTATCT GCTTGCCCAA GGTGCCTTGG ATGTTGATGA CACACAGGTG 
GATGAGTGGC TGGAGTGGGA GGCTACGCAA CTGCAGCCCG TGCTTGGATC GTTCTTGGCA 
TTGTTATACG GGAGTGGCAA AGCCAACGCC GACTGCTTGA AACCGTTGCT GACTCACCTC 
AACAGTTCCC TGCAGGGAAA CCGGTATCTG GTTGGGAATG GCTTATCAGT GGCAGATATC 
AGTGTCTGGT CAGCCTTGTA CCCAGCGCAG GCCACCAGGT CTGATCTCCT CCCCGCCTAC 
CCCCACGTGG AATCCTGGTT CAGGGGCCTC AGCGGGCAGA AGGCGTTTGA AGCCAGCGTT 
GCTGTGGTAA CCCACAGCCA GGGAGCCGGC AGCTTCAAGG CATCCATTTT GGCCCAGCCC 
ATTGTTCAGA TGCCCGTTGG GGATGCCAGG ACGGCAAAAG ACGAAGAGTC TGCCGACACC 
GAGGACGTCA CGAGTGAAGA GATTGAGCGT GCCAGGCAGG CTTGGTTGCT CGGTAAAGCC 
GACGCACCGA CGCCGAGGGC GAGAAACCAT CCGCTTCTTC CGGAGAAGGA TCGGCGCAAC 
GTCTTGATCA CCAGTGCCTT GCCTTACGTC AACAACGTCC CCCACCTGGG CAATATCATC 
GGTTGCGTGC TGAGCGCCGA CGTGTTCTCA AGATTTTGTC GTTTGAGGAA CTACAACTGC 
CTGTACATCT GCGGGACCGA CGAGTACGGG ACGGCCACAG AAACCAAGGC GTTGGCCGAG 
GGGCTCACCC CCCAGCAAAT CTGCGATAAG TACTACCGCA TCCACTATGA CGTCTACACC 
TGGTTCAGCA TCGACTTTGA TTATTTTGGG AGGACGACAA CCAAACAGCA AACGGAGATT 
GCCCAGGATA TTTTTCTCCG TCTCCACCAG CGCGGCTTCC TTCTCAAGGA CACGGTGGAG 
CAGCTGCTGT GTGTCAAGTG CAATCGGTTC CTGGCTGACC GCTTTGTGGA GGGCACCTGC 
CCCCTGTGCT ACTATCCCGA CGCCAGGGGG GACCAGTGCG ACAAGTGTGG CAAACTCATC 
AATGCCACAG AGCTCAAGAA ACCCCGCTGC AAAGTCTGCA AGAAGTCCCC CATCATCAAG 
AACTCGGAGC ACCTCTTCCT GGACCTGCCC AAGCTGCAGC CCCAGGTTCA GGCCTACTTC 
GAGCGGGTGG CAGCAGCGGG TGACTGGACA GCCAATGCCA AGTACGTCAC CTCCAGCTGG 
CTGCGGGATG GCCTCAAGCC CCGCTGCATC ACCAGGGACC TGAAGTGGGG CACCCCGGTG 
CCCCTGGATG GCTTCCGGGA TAAGGTGTTC TACGTCTGGT TTGACGCGCC CATCGGCTAC 
CCGTCCATCA CGGCCAACTA CACGGACCAG TGGGAGAGGT GGTGGAAAAA CCCAGAGGAG 
GTGGAACTGT ACAACTTCAT GGCCAAAGAC AACATCCCAT TCCACAGTGT GGTGTTTCCC 
TGCACGCTGA TTGGAGCTGA GGACAACTAC ACCATCGTCA ATCACCTCAT TGCAGCTGAA 
TACCTGAATT ACGAAGACGG CAAGTTCTCC AAGAGCCGGG GGATCGGCGT GTTTGGCGAC 
ATGGCCAAGG ACACAGGCAT CCCTAGTGAC ATCTGGCGCT TCTACCTGCT GTACGTACGC 
CCAGAGACCA TGGACAGTAC CTTCAGCTGG GCAGACCTCA TGGTGAAGAA TAACAGCGAA 
CTGCTGAATA ATCTTGGTAA CTTCATCAAC AGGTCCCTGA CATTCCTCGA GAAGAATTTT 
GGCAGTGTCG TCCCCGAGAT GACCCTGGAG GAGGACGACG AGCGGTTGCT GGCACTGGTC 
ACTCGCGAGT TGACCTCCTA CAAGCAGCTG CTGGAGAAGT CCAAGATCCG GGATGCCCTG 
CGCCACATCC TCAACATCTC CCGCCTGGGC AACCAGCACA TACAGGCCAA TCAGCCCTGG 
GTGCTGGTCA AGGGTGGAGA CAAGGAGAGA GCCAGAGCGG GCGGCGTGAT TGGCCTGTGC 
ACGAACATCT CCTGCCTGGT ATCCACCATG CTGCTGCCCT ACATGCCGTT AACCAGCCGC 
ACCATCCAGG AGCAGCTCAA CGCTCCGCCG GAATGCAACG TCATCACTGA GAAGTTTGTA 
TGCTACCTGG AGACAGGCCA CAAGATCGGG ACGCCCAAGC CACTATTTGG CAAGTTAGAA 
CCTTCCCTAA TTGAGAGTCT CAAGAAGAAA TTCTCCGGTC AGCAGAAACA GGTTGGTGAC 
TCTGGTGACC CAAAAGCAGC ACCAGTTAGT TCAGTGCCCG TTGACGGCCC AGCTGCACCG 
GCTGGCGATG CCAGTGAGGT CAAACGGCTG ACCGATGAGG TTGCCAAGCA GGGAAACATC 
GTGCGCAAGC TGAAGTCAGA GAAGGCCGAC AAATCAGAGA TCGAGAAGGA GGTGAAGAAG 
CTGCTGGCCC TCAAGCAAGA GTTGGCACTG ACCTCGGGTC AGGACCCGAC CCCTGTGGAA 
TCCTCCAAGG GGAAGAGGAA AAAAGGAGGA AAGAAGAAAG AGGAAAAATC AAAAAACTCT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022109155.1
CDS	122..2887
Translation	MKLLTDKGNQHCLKAVIAACLAGVKLDVEEVENTDHVVPYLRKNRLPVLNLGNGDFIFSANSICRYLLAQGALDVDDTQVDEWLEWEATQLQPVLGSFLALLYGSGKANADCLKPLLTHLNSSLQGNRYLVGNGLSVADISVWSALYPAQATRSDLLPAYPHVESWFRGLSGQKAFEASVAVVTHSQGAGSFKASILAQPIVQMPVGDARTAKDEESADTEDVTSEEIERARQAWLLGKADAPTPRARNHPLLPEKDRRNVLITSALPYVNNVPHLGNIIGCVLSADVFSRFCRLRNYNCLYICGTDEYGTATETKALAEGLTPQQICDKYYRIHYDVYTWFSIDFDYFGRTTTKQQTEIAQDIFLRLHQRGFLLKDTVEQLLCVKCNRFLADRFVEGTCPLCYYPDARGDQCDKCGKLINATELKKPRCKVCKKSPIIKNSEHLFLDLPKLQPQVQAYFERVAAAGDWTANAKYVTSSWLRDGLKPRCITRDLKWGTPVPLDGFRDKVFYVWFDAPIGYPSITANYTDQWERWWKNPEEVELYNFMAKDNIPFHSVVFPCTLIGAEDNYTIVNHLIAAEYLNYEDGKFSKSRGIGVFGDMAKDTGIPSDIWRFYLLYVRPETMDSTFSWADLMVKNNSELLNNLGNFINRSLTFLEKNFGSVVPEMTLEEDDERLLALVTRELTSYKQLLEKSKIRDALRHILNISRLGNQHIQANQPWVLVKGGDKERARAGGVIGLCTNISCLVSTMLLPYMPLTSRTIQEQLNAPPECNVITEKFVCYLETGHKIGTPKPLFGKLEPSLIESLKKKFSGQQKQVGDSGDPKAAPVSSVPVDGPAAPAGDASEVKRLTDEVAKQGNIVRKLKSEKADKSEIEKEVKKLLALKQELALTSGQDPTPVESSKGKRKKGGKKKEEKSKNSE

Target ORF information:

RefSeq Version	XM_022253463.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci methionine--tRNA ligase, cytoplasmic-like (LOC110989230), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022253463.1

ORF Insert Sequence:

ATGAAACTAT TGACAGATAA AGGGAATCAG CACTGTCTTA AGGCTGTTAT TGCTGCATGT 
CTTGCAGGGG TTAAACTGGA CGTGGAAGAA GTTGAAAACA CGGATCATGT TGTGCCTTAC 
CTGAGGAAGA ATCGTTTGCC AGTTCTCAAT CTGGGCAATG GAGATTTCAT TTTTTCTGCA 
AACAGTATAT GCAGGTATCT GCTTGCCCAA GGTGCCTTGG ATGTTGATGA CACACAGGTG 
GATGAGTGGC TGGAGTGGGA GGCTACGCAA CTGCAGCCCG TGCTTGGATC GTTCTTGGCA 
TTGTTATACG GGAGTGGCAA AGCCAACGCC GACTGCTTGA AACCGTTGCT GACTCACCTC 
AACAGTTCCC TGCAGGGAAA CCGGTATCTG GTTGGGAATG GCTTATCAGT GGCAGATATC 
AGTGTCTGGT CAGCCTTGTA CCCAGCGCAG GCCACCAGGT CTGATCTCCT CCCCGCCTAC 
CCCCACGTGG AATCCTGGTT CAGGGGCCTC AGCGGGCAGA AGGCGTTTGA AGCCAGCGTT 
GCTGTGGTAA CCCACAGCCA GGGAGCCGGC AGCTTCAAGG CATCCATTTT GGCCCAGCCC 
ATTGTTCAGA TGCCCGTTGG GGATGCCAGG ACGGCAAAAG ACGAAGAGTC TGCCGACACC 
GAGGACGTCA CGAGTGAAGA GATTGAGCGT GCCAGGCAGG CTTGGTTGCT CGGTAAAGCC 
GACGCACCGA CGCCGAGGGC GAGAAACCAT CCGCTTCTTC CGGAGAAGGA TCGGCGCAAC 
GTCTTGATCA CCAGTGCCTT GCCTTACGTC AACAACGTCC CCCACCTGGG CAATATCATC 
GGTTGCGTGC TGAGCGCCGA CGTGTTCTCA AGATTTTGTC GTTTGAGGAA CTACAACTGC 
CTGTACATCT GCGGGACCGA CGAGTACGGG ACGGCCACAG AAACCAAGGC GTTGGCCGAG 
GGGCTCACCC CCCAGCAAAT CTGCGATAAG TACTACCGCA TCCACTATGA CGTCTACACC 
TGGTTCAGCA TCGACTTTGA TTATTTTGGG AGGACGACAA CCAAACAGCA AACGGAGATT 
GCCCAGGATA TTTTTCTCCG TCTCCACCAG CGCGGCTTCC TTCTCAAGGA CACGGTGGAG 
CAGCTGCTGT GTGTCAAGTG CAATCGGTTC CTGGCTGACC GCTTTGTGGA GGGCACCTGC 
CCCCTGTGCT ACTATCCCGA CGCCAGGGGG GACCAGTGCG ACAAGTGTGG CAAACTCATC 
AATGCCACAG AGCTCAAGAA ACCCCGCTGC AAAGTCTGCA AGAAGTCCCC CATCATCAAG 
AACTCGGAGC ACCTCTTCCT GGACCTGCCC AAGCTGCAGC CCCAGGTTCA GGCCTACTTC 
GAGCGGGTGG CAGCAGCGGG TGACTGGACA GCCAATGCCA AGTACGTCAC CTCCAGCTGG 
CTGCGGGATG GCCTCAAGCC CCGCTGCATC ACCAGGGACC TGAAGTGGGG CACCCCGGTG 
CCCCTGGATG GCTTCCGGGA TAAGGTGTTC TACGTCTGGT TTGACGCGCC CATCGGCTAC 
CCGTCCATCA CGGCCAACTA CACGGACCAG TGGGAGAGGT GGTGGAAAAA CCCAGAGGAG 
GTGGAACTGT ACAACTTCAT GGCCAAAGAC AACATCCCAT TCCACAGTGT GGTGTTTCCC 
TGCACGCTGA TTGGAGCTGA GGACAACTAC ACCATCGTCA ATCACCTCAT TGCAGCTGAA 
TACCTGAATT ACGAAGACGG CAAGTTCTCC AAGAGCCGGG GGATCGGCGT GTTTGGCGAC 
ATGGCCAAGG ACACAGGCAT CCCTAGTGAC ATCTGGCGCT TCTACCTGCT GTACGTACGC 
CCAGAGACCA TGGACAGTAC CTTCAGCTGG GCAGACCTCA TGGTGAAGAA TAACAGCGAA 
CTGCTGAATA ATCTTGGTAA CTTCATCAAC AGGTCCCTGA CATTCCTCGA GAAGAATTTT 
GGCAGTGTCG TCCCCGAGAT GACCCTGGAG GAGGACGACG AGCGGTTGCT GGCACTGGTC 
ACTCGCGAGT TGACCTCCTA CAAGCAGCTG CTGGAGAAGT CCAAGATCCG GGATGCCCTG 
CGCCACATCC TCAACATCTC CCGCCTGGGC AACCAGCACA TACAGGCCAA TCAGCCCTGG 
GTGCTGGTCA AGGGTGGAGA CAAGGAGAGA GCCAGAGCGG GCGGCGTGAT TGGCCTGTGC 
ACGAACATCT CCTGCCTGGT ATCCACCATG CTGCTGCCCT ACATGCCGTT AACCAGCCGC 
ACCATCCAGG AGCAGCTCAA CGCTCCGCCG GAATGCAACG TCATCACTGA GAAGTTTGTA 
TGCTACCTGG AGACAGGCCA CAAGATCGGG ACGCCCAAGC CACTATTTGG CAAGTTAGAA 
CCTTCCCTAA TTGAGAGTCT CAAGAAGAAA TTCTCCGGTC AGCAGAAACA GGTTGGTGAC 
TCTGGTGACC CAAAAGCAGC ACCAGTTAGT TCAGTGCCCG TTGACGGCCC AGCTGCACCG 
GCTGGCGATG CCAGTGAGGT CAAACGGCTG ACCGATGAGG TTGCCAAGCA GGGAAACATC 
GTGCGCAAGC TGAAGTCAGA GAAGGCCGAC AAATCAGAGA TCGAGAAGGA GGTGAAGAAG 
CTGCTGGCCC TCAAGCAAGA GTTGGCACTG ACCTCGGGTC AGGACCCGAC CCCTGTGGAA 
TCCTCCAAGG GGAAGAGGAA AAAAGGAGGA AAGAAGAAAG AGGAAAAATC AAAAAACTCT 
GAATGA