LOC110989096 cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110989096
Entrez Gene ID	110989096
Full Name	cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like
Gene Type	protein-coding
Organism	Acanthaster planci(crown-of-thorns starfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022253221.1	XP_022108913.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like isoform X1
XM_022253223.1	XP_022108915.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC110989096 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110989096 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc172238	XM_022253221.1 Latest version!	Acanthaster planci cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like (LOC110989096), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAc172239	XM_022253223.1 Latest version!	Acanthaster planci cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like (LOC110989096), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110989096 ( XM_022253221.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022108913.1 Acanthaster planci cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like (LOC110989096), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc172238
Clone ID Related Accession (Same CDS sequence)	XM_022253221.1
Accession Version	XM_022253221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091625.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG GCCGATCGTT CAGCGTTCGC TATCCTCCAA ACCGGGCCGA TCTGGGGCTT 
GATTCGGGAG AAAATAACGG GTATATTCGA ACCACCGTGT CTCCGATGAA CCGGGAGAGC 
TACAACCCTT ACTGGGCTTG GTTAGGGCTG AATGACTGGA AGAAGTACGG AATCAACCCG 
CGGACTGTGT TGGCGGGAGC AGCAGCCGTC CTGGCCGTAG CGATGTACCT CTTGCAGGAG 
AGCTTGCTCG CTTTGCTCCA CACGCTATGC GGTCTCTTCG CGCCGGTTTT CAGCATCGTC 
TGCGCTTTCT ACTGGGTGGC CCTGTACCTG AGAAAGTCCT GGACCGGCAC CAATGTCTAT 
GTGTTTTTCG TGGCCTGCGT CTTTGGTGAG ATCGCTGGAC AGGTGGTGTT CGGCACAGAC 
AACATTTACC GTGCCTGGTT GGTGGGCTGC GTCCTGGGCA CGGCCAGCCT GGCAAGCCTC 
ACCTCCACCT TGCCCACGTC GCGCTCGGCG CTGCTCCTCA CGTTCCTCAG TTTTGTCCGA 
TTCTTGAGCG GTTCCACCCT GCATGGATTG CGGCCCTGGT TGAGGCCCTA CGTGGCCTAC 
CTCTGCGGGA TTTTAGGATG CATTTTGTCC AAGTACACAG AAACGCACGT CTTCAAACAG 
TCCCAGGTGG TGAACCATCT CACTTCGGAT GGCAAGATAC CGGTCATCAG GAGACGCAGG 
ACCTCCAGCC AGTCCAGCAC GGCCAGTCTT AGCCACAAAA CACGGCGGAC GTCGCTTCCT 
GCGCTCTCGC AGAAAAATCA AAATGGCGAA TATGAGGTCG ATTTGAACCA CTTTTTAAGC 
AGTCTGGTAG ATAACAACAA CATGGTATGC AACTCAGTGG TGGACATGGC GGTCATGCAG 
GAGGCCCACG GCCTGATCAT GGACATGCTG GCCGACTCCA GTCTGCCGCC CAACGTGGTC 
AGCGGGCTCA AGACGGTCAG CTCCCTGCTC AGCCCACCCA CCAGCTACAA CACGCTGCAG 
CGGCCCAAGA TCAGCCCACT GGTGGCGTTG AGTGAGAGCA ACTACGGCGC GGCTGACATT 
GACGATTCTC CCTATGTGGG TGAAAGGCCT TTGTCGTTAC CCAAGAGATT AAGACGTAGT 
TTGCCTCCAA GTCTCATACG ACGGATGTCG TCAACATGGA CAACAACTAC CTCAGCGACA 
GGCCTTCCAA CTCTTGAGCC ACAACCTCTG CGGACACGTA GTTCCTCATT TCGCCACACC 
CGAGAAAACA ATAATTCAGC CACACCCAGT CCATCAGGAA GTCGCAGCAG TAGCCCCTCC 
CCAGGCTCCC AACAGACCAG CGCCAGCTCA GCCAAGGGCC GCTCCTACTC GGTCGGAACT 
GGTGGAATCA GCCCCAGAGG GCGCTCTCCC AGCCCACTGC TGTCACTGGA CTCGCAGAGG 
TCACTGCAGT CCAAGTCATT GGCTCCGCGA CAGTCGCTGG GCGGGGGCGG CCCGACAGAC 
GACACCAGTG CAACGAGCAC ATCCAGCGCT GCTGAGAGCA GCTTTTTCCC GAGTTCGGAG 
GGAGAGTTCA CCAATGAGGA CGAGTTGGCA GTTAGCTGTC AGGAAGCTCC CTCGCTGCCC 
AGCCGGGCCT TGACATATCC CGAAGCCGAC CAGCACCTAG CCCGAGATGA AGACGGGCAT 
CGTGTGGAGG ACACTGCATC CGGCTTCCAA GAAGCCACTC CTGCAGAACA GTTCACGGAC 
ACTGATGCCA AGGGGACCCG AACGAGCCCT CCCCCAGCAC CCACTCAGAA AAAGCGGGGC 
AGTGTGACAA TAAGTGAACA TGTGACTGTT ATCGATGATG GAAACACAAA GTTGGTACCC 
ACAGACGAGT GTGATGTAGA GATCAATGGA ACAATCGAAG GGAACCAGAT ATTGCCCGAA 
GTACATATAC AGGAAGACTC CAATAGTCTG TCGTCAGAGG AGTTTGATCC GACGTTACCG 
GAATCTCAAG AGTACCTCCA GCGGTTAGAC GAGTGGGACT TTGCCATCTT TGAGCTGGCC 
CAGGCATCCC ACGGCGAGAT CCTTAGTCAG GTTGCCCACA GAGTCTTCAC CGAAGCTGGC 
ATCTTTGAGA CATTCCGGAT ACCGGTCCGA GAATTCATGA ACTACTTTCG GGCATTGGAG 
AACGGCTACC GGCATTTACC ATATCATAAT CGTATTCATG CCGCTGATGT GCTCCATGCA 
GTGTACTACA TGTCAACTCA GCAGATCCCA GGCTTTAGGC AAGTCAACCC GGAGGATTTG 
GATCCCCATT CTAGAACAGG CAAAGACGAC GGGGACAGTT TCACCTCCTA CCACACCCCG 
ACCGTCACGC CGCGCGCCAG CATCTGCAAT GACTCCAGCT ATGGTATCTT AGCCGGTAAC 
CTGCCTGCTC TAGAGCTGAT GGCGCTGTAC ACGGCCGCGG CCATGCATGA CTATGACCAC 
CCAGGACGAA CCAATGCCTT CCTGGTGGCA ACAAGTGCTC CACAGGCTAT TCTCTACAAT 
GATCGCTCCG TCTTGGAGAA CCATCACGCA GCCGCCTCCT GGAGCCTCCT CATCTCCAAG 
CCAGAGTTCA ACTTCCTGTC GTGTCTGGAT GACGCCGAGT TCCGTCGCTT CAGGTTCTTG 
GTGGTGGAGT TCATCCTGGC CACTGATCTC AAGAGGCATT TTGACTTCCT CGTCGAGTTT 
AATGCCAAGG TCAACGATGT AGATGCGTCC GGCATCAATT GGGCATCGGA GGGTGACCGC 
TTGTTGGTCA GTCAGATGTG TATCAAGCTG GCGGACATTG CAGGGCCGAC CAAGACTTTT 
GACCTCCACT ACTGCTGGAC AATGGGCATA TCAGAGGAGT TCTATGAACA GGGTGACGAT 
GAGAGGAAAC TGGGTCTGCC AGTTTCCCCC TACATGGACC GGGAGGCACC CCAGCTGGCC 
AAACTCCAGG AGGGTTTCAT CAACCACCTT GTGGCCCCGC TCTGCAATGC CTACGGCTCT 
GCTGGCCTGC TGCCGGGCAG GTGGCTGGAC GGGGAAGAAA ATGAAGATGA GAAATCGGAG 
GCCGGTGAAG AGACAGCTGT CCCTCTGCCA CAGCAAGAGA TCAAGGAAGA GGGTGATGAT 
ATAGAGGAGG AAGATACATC AAGCGAAGGG GGACCAATGG AGTCCAAGCT GCGACTGAAG 
AGCGGCAAGA CCAAACAGCG CAAGATGGCC AGCATCCTGA CCAAGCACCT CAAGGAGAAT 
CATGACCACT GGATCAAGGT GCTGAAGGAG GAGGAGGACG CCAAGAGGCG GGAAGAGAAG 
AAGGAAGGCA AGGAGGAGGG CAGCGGGGGC GGCATGGCAC CCATCCGGGA GGACTCGGAG 
GAGCGCAACT CAGATGAGAA GGGCGATGAG GATGAGCCGG AAAGCAGACC ATCCCCGGAC 
GTGGTGGAGA GGCTCAAAGA GGCAGCAACT GAACGCAGGA AGAGCCTTGA CGACGCCCGG 
ACCTCCAAAG CCAAACAACA CAGGGTCAGC TCAGACGAGG AGCAAGACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022108913.1
CDS	360..3893
Translation	MATGRSFSVRYPPNRADLGLDSGENNGYIRTTVSPMNRESYNPYWAWLGLNDWKKYGINPRTVLAGAAAVLAVAMYLLQESLLALLHTLCGLFAPVFSIVCAFYWVALYLRKSWTGTNVYVFFVACVFGEIAGQVVFGTDNIYRAWLVGCVLGTASLASLTSTLPTSRSALLLTFLSFVRFLSGSTLHGLRPWLRPYVAYLCGILGCILSKYTETHVFKQSQVVNHLTSDGKIPVIRRRRTSSQSSTASLSHKTRRTSLPALSQKNQNGEYEVDLNHFLSSLVDNNNMVCNSVVDMAVMQEAHGLIMDMLADSSLPPNVVSGLKTVSSLLSPPTSYNTLQRPKISPLVALSESNYGAADIDDSPYVGERPLSLPKRLRRSLPPSLIRRMSSTWTTTTSATGLPTLEPQPLRTRSSSFRHTRENNNSATPSPSGSRSSSPSPGSQQTSASSAKGRSYSVGTGGISPRGRSPSPLLSLDSQRSLQSKSLAPRQSLGGGGPTDDTSATSTSSAAESSFFPSSEGEFTNEDELAVSCQEAPSLPSRALTYPEADQHLARDEDGHRVEDTASGFQEATPAEQFTDTDAKGTRTSPPPAPTQKKRGSVTISEHVTVIDDGNTKLVPTDECDVEINGTIEGNQILPEVHIQEDSNSLSSEEFDPTLPESQEYLQRLDEWDFAIFELAQASHGEILSQVAHRVFTEAGIFETFRIPVREFMNYFRALENGYRHLPYHNRIHAADVLHAVYYMSTQQIPGFRQVNPEDLDPHSRTGKDDGDSFTSYHTPTVTPRASICNDSSYGILAGNLPALELMALYTAAAMHDYDHPGRTNAFLVATSAPQAILYNDRSVLENHHAAASWSLLISKPEFNFLSCLDDAEFRRFRFLVVEFILATDLKRHFDFLVEFNAKVNDVDASGINWASEGDRLLVSQMCIKLADIAGPTKTFDLHYCWTMGISEEFYEQGDDERKLGLPVSPYMDREAPQLAKLQEGFINHLVAPLCNAYGSAGLLPGRWLDGEENEDEKSEAGEETAVPLPQQEIKEEGDDIEEEDTSSEGGPMESKLRLKSGKTKQRKMASILTKHLKENHDHWIKVLKEEEDAKRREEKKEGKEEGSGGGMAPIREDSEERNSDEKGDEDEPESRPSPDVVERLKEAATERRKSLDDARTSKAKQHRVSSDEEQDR

Target ORF information:

RefSeq Version	XM_022253221.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like (LOC110989096), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022253221.1

ORF Insert Sequence:

ATGGCGACCG GCCGATCGTT CAGCGTTCGC TATCCTCCAA ACCGGGCCGA TCTGGGGCTT 
GATTCGGGAG AAAATAACGG GTATATTCGA ACCACCGTGT CTCCGATGAA CCGGGAGAGC 
TACAACCCTT ACTGGGCTTG GTTAGGGCTG AATGACTGGA AGAAGTACGG AATCAACCCG 
CGGACTGTGT TGGCGGGAGC AGCAGCCGTC CTGGCCGTAG CGATGTACCT CTTGCAGGAG 
AGCTTGCTCG CTTTGCTCCA CACGCTATGC GGTCTCTTCG CGCCGGTTTT CAGCATCGTC 
TGCGCTTTCT ACTGGGTGGC CCTGTACCTG AGAAAGTCCT GGACCGGCAC CAATGTCTAT 
GTGTTTTTCG TGGCCTGCGT CTTTGGTGAG ATCGCTGGAC AGGTGGTGTT CGGCACAGAC 
AACATTTACC GTGCCTGGTT GGTGGGCTGC GTCCTGGGCA CGGCCAGCCT GGCAAGCCTC 
ACCTCCACCT TGCCCACGTC GCGCTCGGCG CTGCTCCTCA CGTTCCTCAG TTTTGTCCGA 
TTCTTGAGCG GTTCCACCCT GCATGGATTG CGGCCCTGGT TGAGGCCCTA CGTGGCCTAC 
CTCTGCGGGA TTTTAGGATG CATTTTGTCC AAGTACACAG AAACGCACGT CTTCAAACAG 
TCCCAGGTGG TGAACCATCT CACTTCGGAT GGCAAGATAC CGGTCATCAG GAGACGCAGG 
ACCTCCAGCC AGTCCAGCAC GGCCAGTCTT AGCCACAAAA CACGGCGGAC GTCGCTTCCT 
GCGCTCTCGC AGAAAAATCA AAATGGCGAA TATGAGGTCG ATTTGAACCA CTTTTTAAGC 
AGTCTGGTAG ATAACAACAA CATGGTATGC AACTCAGTGG TGGACATGGC GGTCATGCAG 
GAGGCCCACG GCCTGATCAT GGACATGCTG GCCGACTCCA GTCTGCCGCC CAACGTGGTC 
AGCGGGCTCA AGACGGTCAG CTCCCTGCTC AGCCCACCCA CCAGCTACAA CACGCTGCAG 
CGGCCCAAGA TCAGCCCACT GGTGGCGTTG AGTGAGAGCA ACTACGGCGC GGCTGACATT 
GACGATTCTC CCTATGTGGG TGAAAGGCCT TTGTCGTTAC CCAAGAGATT AAGACGTAGT 
TTGCCTCCAA GTCTCATACG ACGGATGTCG TCAACATGGA CAACAACTAC CTCAGCGACA 
GGCCTTCCAA CTCTTGAGCC ACAACCTCTG CGGACACGTA GTTCCTCATT TCGCCACACC 
CGAGAAAACA ATAATTCAGC CACACCCAGT CCATCAGGAA GTCGCAGCAG TAGCCCCTCC 
CCAGGCTCCC AACAGACCAG CGCCAGCTCA GCCAAGGGCC GCTCCTACTC GGTCGGAACT 
GGTGGAATCA GCCCCAGAGG GCGCTCTCCC AGCCCACTGC TGTCACTGGA CTCGCAGAGG 
TCACTGCAGT CCAAGTCATT GGCTCCGCGA CAGTCGCTGG GCGGGGGCGG CCCGACAGAC 
GACACCAGTG CAACGAGCAC ATCCAGCGCT GCTGAGAGCA GCTTTTTCCC GAGTTCGGAG 
GGAGAGTTCA CCAATGAGGA CGAGTTGGCA GTTAGCTGTC AGGAAGCTCC CTCGCTGCCC 
AGCCGGGCCT TGACATATCC CGAAGCCGAC CAGCACCTAG CCCGAGATGA AGACGGGCAT 
CGTGTGGAGG ACACTGCATC CGGCTTCCAA GAAGCCACTC CTGCAGAACA GTTCACGGAC 
ACTGATGCCA AGGGGACCCG AACGAGCCCT CCCCCAGCAC CCACTCAGAA AAAGCGGGGC 
AGTGTGACAA TAAGTGAACA TGTGACTGTT ATCGATGATG GAAACACAAA GTTGGTACCC 
ACAGACGAGT GTGATGTAGA GATCAATGGA ACAATCGAAG GGAACCAGAT ATTGCCCGAA 
GTACATATAC AGGAAGACTC CAATAGTCTG TCGTCAGAGG AGTTTGATCC GACGTTACCG 
GAATCTCAAG AGTACCTCCA GCGGTTAGAC GAGTGGGACT TTGCCATCTT TGAGCTGGCC 
CAGGCATCCC ACGGCGAGAT CCTTAGTCAG GTTGCCCACA GAGTCTTCAC CGAAGCTGGC 
ATCTTTGAGA CATTCCGGAT ACCGGTCCGA GAATTCATGA ACTACTTTCG GGCATTGGAG 
AACGGCTACC GGCATTTACC ATATCATAAT CGTATTCATG CCGCTGATGT GCTCCATGCA 
GTGTACTACA TGTCAACTCA GCAGATCCCA GGCTTTAGGC AAGTCAACCC GGAGGATTTG 
GATCCCCATT CTAGAACAGG CAAAGACGAC GGGGACAGTT TCACCTCCTA CCACACCCCG 
ACCGTCACGC CGCGCGCCAG CATCTGCAAT GACTCCAGCT ATGGTATCTT AGCCGGTAAC 
CTGCCTGCTC TAGAGCTGAT GGCGCTGTAC ACGGCCGCGG CCATGCATGA CTATGACCAC 
CCAGGACGAA CCAATGCCTT CCTGGTGGCA ACAAGTGCTC CACAGGCTAT TCTCTACAAT 
GATCGCTCCG TCTTGGAGAA CCATCACGCA GCCGCCTCCT GGAGCCTCCT CATCTCCAAG 
CCAGAGTTCA ACTTCCTGTC GTGTCTGGAT GACGCCGAGT TCCGTCGCTT CAGGTTCTTG 
GTGGTGGAGT TCATCCTGGC CACTGATCTC AAGAGGCATT TTGACTTCCT CGTCGAGTTT 
AATGCCAAGG TCAACGATGT AGATGCGTCC GGCATCAATT GGGCATCGGA GGGTGACCGC 
TTGTTGGTCA GTCAGATGTG TATCAAGCTG GCGGACATTG CAGGGCCGAC CAAGACTTTT 
GACCTCCACT ACTGCTGGAC AATGGGCATA TCAGAGGAGT TCTATGAACA GGGTGACGAT 
GAGAGGAAAC TGGGTCTGCC AGTTTCCCCC TACATGGACC GGGAGGCACC CCAGCTGGCC 
AAACTCCAGG AGGGTTTCAT CAACCACCTT GTGGCCCCGC TCTGCAATGC CTACGGCTCT 
GCTGGCCTGC TGCCGGGCAG GTGGCTGGAC GGGGAAGAAA ATGAAGATGA GAAATCGGAG 
GCCGGTGAAG AGACAGCTGT CCCTCTGCCA CAGCAAGAGA TCAAGGAAGA GGGTGATGAT 
ATAGAGGAGG AAGATACATC AAGCGAAGGG GGACCAATGG AGTCCAAGCT GCGACTGAAG 
AGCGGCAAGA CCAAACAGCG CAAGATGGCC AGCATCCTGA CCAAGCACCT CAAGGAGAAT 
CATGACCACT GGATCAAGGT GCTGAAGGAG GAGGAGGACG CCAAGAGGCG GGAAGAGAAG 
AAGGAAGGCA AGGAGGAGGG CAGCGGGGGC GGCATGGCAC CCATCCGGGA GGACTCGGAG 
GAGCGCAACT CAGATGAGAA GGGCGATGAG GATGAGCCGG AAAGCAGACC ATCCCCGGAC 
GTGGTGGAGA GGCTCAAAGA GGCAGCAACT GAACGCAGGA AGAGCCTTGA CGACGCCCGG 
ACCTCCAAAG CCAAACAACA CAGGGTCAGC TCAGACGAGG AGCAAGACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110989096 ( XM_022253223.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022108915.1 Acanthaster planci cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like (LOC110989096), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc172239
Clone ID Related Accession (Same CDS sequence)	XM_022253223.1
Accession Version	XM_022253223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091625.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG GCCGATCGTT CAGCGTTCGC TATCCTCCAA ACCGGGCCGA TCTGGGGCTT 
GATTCGGGAG AAAATAACGG GTATATTCGA ACCACCGTGT CTCCGATGAA CCGGGAGAGC 
TACAACCCTT ACTGGGCTTG GTTAGGGCTG AATGACTGGA AGAAGTACGG AATCAACCCG 
CGGACTGTGT TGGCGGGAGC AGCAGCCGTC CTGGCCGTAG CGATGTACCT CTTGCAGGAG 
AGCTTGCTCG CTTTGCTCCA CACGCTATGC GGTCTCTTCG CGCCGGTTTT CAGCATCGTC 
TGCGCTTTCT ACTGGGTGGC CCTGTACCTG AGAAAGTCCT GGACCGGCAC CAATGTCTAT 
GTGTTTTTCG TGGCCTGCGT CTTTGGTGAG ATCGCTGGAC AGGTGGTGTT CGGCACAGAC 
AACATTTACC GTGCCTGGTT GGTGGGCTGC GTCCTGGGCA CGGCCAGCCT GGCAAGCCTC 
ACCTCCACCT TGCCCACGTC GCGCTCGGCG CTGCTCCTCA CGTTCCTCAG TTTTGTCCGA 
TTCTTGAGCG GTTCCACCCT GCATGGATTG CGGCCCTGGT TGAGGCCCTA CGTGGCCTAC 
CTCTGCGGGA TTTTAGGATG CATTTTGTCC AAGTACACAG AAACGCACGT CTTCAAACAG 
TCCCAGGTGG TGAACCATCT CACTTCGGAT GGCAAGATAC CGGTCATCAG GAGACGCAGG 
ACCTCCAGCC AGTCCAGCAC GGCCAGTCTT AGCCACAAAA CACGGCGGAC GTCGCTTCCT 
GCGCTCTCGC AGAAAAATCA AGTATGCAAC TCAGTGGTGG ACATGGCGGT CATGCAGGAG 
GCCCACGGCC TGATCATGGA CATGCTGGCC GACTCCAGTC TGCCGCCCAA CGTGGTCAGC 
GGGCTCAAGA CGGTCAGCTC CCTGCTCAGC CCACCCACCA GCTACAACAC GCTGCAGCGG 
CCCAAGATCA GCCCACTGGT GGCGTTGAGT GAGAGCAACT ACGGCGCGGC TGACATTGAC 
GATTCTCCCT ATGTGGGTGA AAGGCCTTTG TCGTTACCCA AGAGATTAAG ACGTAGTTTG 
CCTCCAAGTC TCATACGACG GATGTCGTCA ACATGGACAA CAACTACCTC AGCGACAGGC 
CTTCCAACTC TTGAGCCACA ACCTCTGCGG ACACGTAGTT CCTCATTTCG CCACACCCGA 
GAAAACAATA ATTCAGCCAC ACCCAGTCCA TCAGGAAGTC GCAGCAGTAG CCCCTCCCCA 
GGCTCCCAAC AGACCAGCGC CAGCTCAGCC AAGGGCCGCT CCTACTCGGT CGGAACTGGT 
GGAATCAGCC CCAGAGGGCG CTCTCCCAGC CCACTGCTGT CACTGGACTC GCAGAGGTCA 
CTGCAGTCCA AGTCATTGGC TCCGCGACAG TCGCTGGGCG GGGGCGGCCC GACAGACGAC 
ACCAGTGCAA CGAGCACATC CAGCGCTGCT GAGAGCAGCT TTTTCCCGAG TTCGGAGGGA 
GAGTTCACCA ATGAGGACGA GTTGGCAGTT AGCTGTCAGG AAGCTCCCTC GCTGCCCAGC 
CGGGCCTTGA CATATCCCGA AGCCGACCAG CACCTAGCCC GAGATGAAGA CGGGCATCGT 
GTGGAGGACA CTGCATCCGG CTTCCAAGAA GCCACTCCTG CAGAACAGTT CACGGACACT 
GATGCCAAGG GGACCCGAAC GAGCCCTCCC CCAGCACCCA CTCAGAAAAA GCGGGGCAGT 
GTGACAATAA GTGAACATGT GACTGTTATC GATGATGGAA ACACAAAGTT GGTACCCACA 
GACGAGTGTG ATGTAGAGAT CAATGGAACA ATCGAAGGGA ACCAGATATT GCCCGAAGTA 
CATATACAGG AAGACTCCAA TAGTCTGTCG TCAGAGGAGT TTGATCCGAC GTTACCGGAA 
TCTCAAGAGT ACCTCCAGCG GTTAGACGAG TGGGACTTTG CCATCTTTGA GCTGGCCCAG 
GCATCCCACG GCGAGATCCT TAGTCAGGTT GCCCACAGAG TCTTCACCGA AGCTGGCATC 
TTTGAGACAT TCCGGATACC GGTCCGAGAA TTCATGAACT ACTTTCGGGC ATTGGAGAAC 
GGCTACCGGC ATTTACCATA TCATAATCGT ATTCATGCCG CTGATGTGCT CCATGCAGTG 
TACTACATGT CAACTCAGCA GATCCCAGGC TTTAGGCAAG TCAACCCGGA GGATTTGGAT 
CCCCATTCTA GAACAGGCAA AGACGACGGG GACAGTTTCA CCTCCTACCA CACCCCGACC 
GTCACGCCGC GCGCCAGCAT CTGCAATGAC TCCAGCTATG GTATCTTAGC CGGTAACCTG 
CCTGCTCTAG AGCTGATGGC GCTGTACACG GCCGCGGCCA TGCATGACTA TGACCACCCA 
GGACGAACCA ATGCCTTCCT GGTGGCAACA AGTGCTCCAC AGGCTATTCT CTACAATGAT 
CGCTCCGTCT TGGAGAACCA TCACGCAGCC GCCTCCTGGA GCCTCCTCAT CTCCAAGCCA 
GAGTTCAACT TCCTGTCGTG TCTGGATGAC GCCGAGTTCC GTCGCTTCAG GTTCTTGGTG 
GTGGAGTTCA TCCTGGCCAC TGATCTCAAG AGGCATTTTG ACTTCCTCGT CGAGTTTAAT 
GCCAAGGTCA ACGATGTAGA TGCGTCCGGC ATCAATTGGG CATCGGAGGG TGACCGCTTG 
TTGGTCAGTC AGATGTGTAT CAAGCTGGCG GACATTGCAG GGCCGACCAA GACTTTTGAC 
CTCCACTACT GCTGGACAAT GGGCATATCA GAGGAGTTCT ATGAACAGGG TGACGATGAG 
AGGAAACTGG GTCTGCCAGT TTCCCCCTAC ATGGACCGGG AGGCACCCCA GCTGGCCAAA 
CTCCAGGAGG GTTTCATCAA CCACCTTGTG GCCCCGCTCT GCAATGCCTA CGGCTCTGCT 
GGCCTGCTGC CGGGCAGGTG GCTGGACGGG GAAGAAAATG AAGATGAGAA ATCGGAGGCC 
GGTGAAGAGA CAGCTGTCCC TCTGCCACAG CAAGAGATCA AGGAAGAGGG TGATGATATA 
GAGGAGGAAG ATACATCAAG CGAAGGGGGA CCAATGGAGT CCAAGCTGCG ACTGAAGAGC 
GGCAAGACCA AACAGCGCAA GATGGCCAGC ATCCTGACCA AGCACCTCAA GGAGAATCAT 
GACCACTGGA TCAAGGTGCT GAAGGAGGAG GAGGACGCCA AGAGGCGGGA AGAGAAGAAG 
GAAGGCAAGG AGGAGGGCAG CGGGGGCGGC ATGGCACCCA TCCGGGAGGA CTCGGAGGAG 
CGCAACTCAG ATGAGAAGGG CGATGAGGAT GAGCCGGAAA GCAGACCATC CCCGGACGTG 
GTGGAGAGGC TCAAAGAGGC AGCAACTGAA CGCAGGAAGA GCCTTGACGA CGCCCGGACC 
TCCAAAGCCA AACAACACAG GGTCAGCTCA GACGAGGAGC AAGACAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022108915.1
CDS	359..3829
Translation	MATGRSFSVRYPPNRADLGLDSGENNGYIRTTVSPMNRESYNPYWAWLGLNDWKKYGINPRTVLAGAAAVLAVAMYLLQESLLALLHTLCGLFAPVFSIVCAFYWVALYLRKSWTGTNVYVFFVACVFGEIAGQVVFGTDNIYRAWLVGCVLGTASLASLTSTLPTSRSALLLTFLSFVRFLSGSTLHGLRPWLRPYVAYLCGILGCILSKYTETHVFKQSQVVNHLTSDGKIPVIRRRRTSSQSSTASLSHKTRRTSLPALSQKNQVCNSVVDMAVMQEAHGLIMDMLADSSLPPNVVSGLKTVSSLLSPPTSYNTLQRPKISPLVALSESNYGAADIDDSPYVGERPLSLPKRLRRSLPPSLIRRMSSTWTTTTSATGLPTLEPQPLRTRSSSFRHTRENNNSATPSPSGSRSSSPSPGSQQTSASSAKGRSYSVGTGGISPRGRSPSPLLSLDSQRSLQSKSLAPRQSLGGGGPTDDTSATSTSSAAESSFFPSSEGEFTNEDELAVSCQEAPSLPSRALTYPEADQHLARDEDGHRVEDTASGFQEATPAEQFTDTDAKGTRTSPPPAPTQKKRGSVTISEHVTVIDDGNTKLVPTDECDVEINGTIEGNQILPEVHIQEDSNSLSSEEFDPTLPESQEYLQRLDEWDFAIFELAQASHGEILSQVAHRVFTEAGIFETFRIPVREFMNYFRALENGYRHLPYHNRIHAADVLHAVYYMSTQQIPGFRQVNPEDLDPHSRTGKDDGDSFTSYHTPTVTPRASICNDSSYGILAGNLPALELMALYTAAAMHDYDHPGRTNAFLVATSAPQAILYNDRSVLENHHAAASWSLLISKPEFNFLSCLDDAEFRRFRFLVVEFILATDLKRHFDFLVEFNAKVNDVDASGINWASEGDRLLVSQMCIKLADIAGPTKTFDLHYCWTMGISEEFYEQGDDERKLGLPVSPYMDREAPQLAKLQEGFINHLVAPLCNAYGSAGLLPGRWLDGEENEDEKSEAGEETAVPLPQQEIKEEGDDIEEEDTSSEGGPMESKLRLKSGKTKQRKMASILTKHLKENHDHWIKVLKEEEDAKRREEKKEGKEEGSGGGMAPIREDSEERNSDEKGDEDEPESRPSPDVVERLKEAATERRKSLDDARTSKAKQHRVSSDEEQDR

Target ORF information:

RefSeq Version	XM_022253223.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci cGMP-inhibited 3',5'-cyclic phosphodiesterase A-like (LOC110989096), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022253223.1

ORF Insert Sequence:

ATGGCGACCG GCCGATCGTT CAGCGTTCGC TATCCTCCAA ACCGGGCCGA TCTGGGGCTT 
GATTCGGGAG AAAATAACGG GTATATTCGA ACCACCGTGT CTCCGATGAA CCGGGAGAGC 
TACAACCCTT ACTGGGCTTG GTTAGGGCTG AATGACTGGA AGAAGTACGG AATCAACCCG 
CGGACTGTGT TGGCGGGAGC AGCAGCCGTC CTGGCCGTAG CGATGTACCT CTTGCAGGAG 
AGCTTGCTCG CTTTGCTCCA CACGCTATGC GGTCTCTTCG CGCCGGTTTT CAGCATCGTC 
TGCGCTTTCT ACTGGGTGGC CCTGTACCTG AGAAAGTCCT GGACCGGCAC CAATGTCTAT 
GTGTTTTTCG TGGCCTGCGT CTTTGGTGAG ATCGCTGGAC AGGTGGTGTT CGGCACAGAC 
AACATTTACC GTGCCTGGTT GGTGGGCTGC GTCCTGGGCA CGGCCAGCCT GGCAAGCCTC 
ACCTCCACCT TGCCCACGTC GCGCTCGGCG CTGCTCCTCA CGTTCCTCAG TTTTGTCCGA 
TTCTTGAGCG GTTCCACCCT GCATGGATTG CGGCCCTGGT TGAGGCCCTA CGTGGCCTAC 
CTCTGCGGGA TTTTAGGATG CATTTTGTCC AAGTACACAG AAACGCACGT CTTCAAACAG 
TCCCAGGTGG TGAACCATCT CACTTCGGAT GGCAAGATAC CGGTCATCAG GAGACGCAGG 
ACCTCCAGCC AGTCCAGCAC GGCCAGTCTT AGCCACAAAA CACGGCGGAC GTCGCTTCCT 
GCGCTCTCGC AGAAAAATCA AGTATGCAAC TCAGTGGTGG ACATGGCGGT CATGCAGGAG 
GCCCACGGCC TGATCATGGA CATGCTGGCC GACTCCAGTC TGCCGCCCAA CGTGGTCAGC 
GGGCTCAAGA CGGTCAGCTC CCTGCTCAGC CCACCCACCA GCTACAACAC GCTGCAGCGG 
CCCAAGATCA GCCCACTGGT GGCGTTGAGT GAGAGCAACT ACGGCGCGGC TGACATTGAC 
GATTCTCCCT ATGTGGGTGA AAGGCCTTTG TCGTTACCCA AGAGATTAAG ACGTAGTTTG 
CCTCCAAGTC TCATACGACG GATGTCGTCA ACATGGACAA CAACTACCTC AGCGACAGGC 
CTTCCAACTC TTGAGCCACA ACCTCTGCGG ACACGTAGTT CCTCATTTCG CCACACCCGA 
GAAAACAATA ATTCAGCCAC ACCCAGTCCA TCAGGAAGTC GCAGCAGTAG CCCCTCCCCA 
GGCTCCCAAC AGACCAGCGC CAGCTCAGCC AAGGGCCGCT CCTACTCGGT CGGAACTGGT 
GGAATCAGCC CCAGAGGGCG CTCTCCCAGC CCACTGCTGT CACTGGACTC GCAGAGGTCA 
CTGCAGTCCA AGTCATTGGC TCCGCGACAG TCGCTGGGCG GGGGCGGCCC GACAGACGAC 
ACCAGTGCAA CGAGCACATC CAGCGCTGCT GAGAGCAGCT TTTTCCCGAG TTCGGAGGGA 
GAGTTCACCA ATGAGGACGA GTTGGCAGTT AGCTGTCAGG AAGCTCCCTC GCTGCCCAGC 
CGGGCCTTGA CATATCCCGA AGCCGACCAG CACCTAGCCC GAGATGAAGA CGGGCATCGT 
GTGGAGGACA CTGCATCCGG CTTCCAAGAA GCCACTCCTG CAGAACAGTT CACGGACACT 
GATGCCAAGG GGACCCGAAC GAGCCCTCCC CCAGCACCCA CTCAGAAAAA GCGGGGCAGT 
GTGACAATAA GTGAACATGT GACTGTTATC GATGATGGAA ACACAAAGTT GGTACCCACA 
GACGAGTGTG ATGTAGAGAT CAATGGAACA ATCGAAGGGA ACCAGATATT GCCCGAAGTA 
CATATACAGG AAGACTCCAA TAGTCTGTCG TCAGAGGAGT TTGATCCGAC GTTACCGGAA 
TCTCAAGAGT ACCTCCAGCG GTTAGACGAG TGGGACTTTG CCATCTTTGA GCTGGCCCAG 
GCATCCCACG GCGAGATCCT TAGTCAGGTT GCCCACAGAG TCTTCACCGA AGCTGGCATC 
TTTGAGACAT TCCGGATACC GGTCCGAGAA TTCATGAACT ACTTTCGGGC ATTGGAGAAC 
GGCTACCGGC ATTTACCATA TCATAATCGT ATTCATGCCG CTGATGTGCT CCATGCAGTG 
TACTACATGT CAACTCAGCA GATCCCAGGC TTTAGGCAAG TCAACCCGGA GGATTTGGAT 
CCCCATTCTA GAACAGGCAA AGACGACGGG GACAGTTTCA CCTCCTACCA CACCCCGACC 
GTCACGCCGC GCGCCAGCAT CTGCAATGAC TCCAGCTATG GTATCTTAGC CGGTAACCTG 
CCTGCTCTAG AGCTGATGGC GCTGTACACG GCCGCGGCCA TGCATGACTA TGACCACCCA 
GGACGAACCA ATGCCTTCCT GGTGGCAACA AGTGCTCCAC AGGCTATTCT CTACAATGAT 
CGCTCCGTCT TGGAGAACCA TCACGCAGCC GCCTCCTGGA GCCTCCTCAT CTCCAAGCCA 
GAGTTCAACT TCCTGTCGTG TCTGGATGAC GCCGAGTTCC GTCGCTTCAG GTTCTTGGTG 
GTGGAGTTCA TCCTGGCCAC TGATCTCAAG AGGCATTTTG ACTTCCTCGT CGAGTTTAAT 
GCCAAGGTCA ACGATGTAGA TGCGTCCGGC ATCAATTGGG CATCGGAGGG TGACCGCTTG 
TTGGTCAGTC AGATGTGTAT CAAGCTGGCG GACATTGCAG GGCCGACCAA GACTTTTGAC 
CTCCACTACT GCTGGACAAT GGGCATATCA GAGGAGTTCT ATGAACAGGG TGACGATGAG 
AGGAAACTGG GTCTGCCAGT TTCCCCCTAC ATGGACCGGG AGGCACCCCA GCTGGCCAAA 
CTCCAGGAGG GTTTCATCAA CCACCTTGTG GCCCCGCTCT GCAATGCCTA CGGCTCTGCT 
GGCCTGCTGC CGGGCAGGTG GCTGGACGGG GAAGAAAATG AAGATGAGAA ATCGGAGGCC 
GGTGAAGAGA CAGCTGTCCC TCTGCCACAG CAAGAGATCA AGGAAGAGGG TGATGATATA 
GAGGAGGAAG ATACATCAAG CGAAGGGGGA CCAATGGAGT CCAAGCTGCG ACTGAAGAGC 
GGCAAGACCA AACAGCGCAA GATGGCCAGC ATCCTGACCA AGCACCTCAA GGAGAATCAT 
GACCACTGGA TCAAGGTGCT GAAGGAGGAG GAGGACGCCA AGAGGCGGGA AGAGAAGAAG 
GAAGGCAAGG AGGAGGGCAG CGGGGGCGGC ATGGCACCCA TCCGGGAGGA CTCGGAGGAG 
CGCAACTCAG ATGAGAAGGG CGATGAGGAT GAGCCGGAAA GCAGACCATC CCCGGACGTG 
GTGGAGAGGC TCAAAGAGGC AGCAACTGAA CGCAGGAAGA GCCTTGACGA CGCCCGGACC 
TCCAAAGCCA AACAACACAG GGTCAGCTCA GACGAGGAGC AAGACAGATG A