LOC110985703 cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110985703
Entrez Gene ID	110985703
Full Name	WASH complex subunit 5-like
Gene Type	protein-coding
Organism	Acanthaster planci(crown-of-thorns starfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022246916.1	XP_022102608.1	WASH complex subunit 5-like
XM_022246917.1	XP_022102609.1	WASH complex subunit 5-like

Pathways from BioSystems
Gene Ontology

The following LOC110985703 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110985703 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc167947	XM_022246917.1 Latest version!	Acanthaster planci WASH complex subunit 5-like (LOC110985703), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAc167947	XM_022246916.1 Latest version!	Acanthaster planci WASH complex subunit 5-like (LOC110985703), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110985703 ( XM_022246916.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022102608.1 Acanthaster planci WASH complex subunit 5-like (LOC110985703), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc167947
Clone ID Related Accession (Same CDS sequence)	XM_022246916.1 , XM_022246917.1
Accession Version	XM_022246916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	WASH complex subunit 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091500.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATT TTCTGGCAGA CAACAACCTT TGTGGACAAA CTCTACTCAA GTTGGTCTCT 
CGTGGCAATG CTATCATTGC TGAGCTACTT CGTTTGGCTG ACTTTATCCC TCCTGTCTTC 
AAGCTTGATA ATAAGTTTGA TCAGATGAAG TATGGGGACA TTCTCTTTGA TTTCAATTAC 
TTCAAGGGAT CAGAATACTA CGATAACAAG ATTGATTCCA GGCCTGAGCT GCAAGACCTT 
GATGAAATCT TCCGGGAAAA TCATATTGAC ATTTTACGGC GCTTCTATCT TGCCTTTGAG 
AGTATCCATA AATATGTGAC TGATCTCAAT CGATTTCTTG AAGATTTGGA GGAAGGGGTT 
TACATTCAGC AAACACTAGA GACTGTGCTA CTCAATGAAG ACGGCAAGCA ACTTCTGTGT 
GAATCCTTGT ATCTATACGG TGTGATGCTG CTTGTGGTTG ATATGAGAAT TGATGGCCCA 
GTCAGAGAAC GAATGTTGGT ATCTTATCAC AGATACAGTG CTGCCAAGGC TGCTGTTGAT 
TCCAACATGG ACGATGTCTG CAAACTATTG CGATCAACTG GATTCTATTC AGCTCCAGGA 
GCAAAAAGAC CTCTTAACTA CCCAGAGAAC TACTTCAAGC GCATTCCGAT ACCCCCTCAT 
TTCATTGAGA TGGTCATTGC CAGGCTACGT TCAGATGACA TATACAACCA GATCTCAGCC 
TATCCCTTAC CAGAGCACCG AAGCACAGCG TTAGCCACAC AAGCAGCCAT GTTATACATC 
AGTCTCTACT TCTCTCCAGA CACACTGCAC GGTCAACAGG CTAAGATGAG GGAAATTGTT 
GACAAACATT TTCCAGATAA TTGGATGATC AGCATTTACA TGGGAATTGT GATCAATCTG 
GCAGACATGT GGGAACCATA CAAAGCAGCA CGAACAGCTC TTAATAACAC CCTGGACTTG 
ACCAATGTCA AGACCTTGAC CGTGAAGCAT AACCAGAAAG TTGACCAGTT GATCAAGCTG 
TTACGTCAGT ATTTGACAGA AGGAACACTT ACAGAGGAAT ATGTCCTGGA CAACATTCCT 
AAGCTGATGA ACTGTCTAAG AGAATGTAAC GTCAATCTGC GCTGGCTTAT GCTACACACT 
GCAGATGGGC CCTGGGATGG TAACAAGAAG ATAAGACAGA TGAAGGAAGC CATGATTTTG 
GAATCCAAGT TTAGTCCTCG GACTTTATTT GAGCTTCTGT TGGATTCAGC ACAATTTGAG 
TTCAAACTCA AGGAGATGTT CAAGAAGATG TTGTCAGAAA AGCAAAAGAA GTGGGAACTG 
TACAAACAAG AGAGTGTAGA TCGAATGACT GAGCTTGGTG AGGTCTTCTC AGGAACCAAA 
CCATTGACTC GCGTTGCCAA GAATGAGAAC CTTCAGGCTT GGTTTGCTGA GATGGCTAAA 
CAGATAAACT CACTCAACTA CGATGACTCT ACTGCTGCAG GTCGCAAGAT TGTCCAACTC 
ATACAGGCCC TGGAAGAGGT GCAAGAGTTT CATCAGTTGG AGAGCAACTT GCAAGTTCGT 
CAGTTTCTGC TGGACACTCG CCAGTTCCTT CATCAAATGA TTCGAACCAT TAACATCAAG 
GAAGAGGTGC TCATCACAAT GCAGATTGTA GCTGATCTGT CTTATGCCTG GGAACTCATT 
GATACGTACA CACCTTACAT GCAGCAGGGC ATCAAAAGCA ACCCAGGTCT GGTTACCAAA 
CTGAGAGCAA CATTCCTGAA GCTGGCATCA GCTCTTGATC TGCCTCTGAT ACGAATCAAT 
CAAGCCAACA GTCCTGACCT GATAAGTGTC TCTCAATACT ACTCCAGTGA GCTTGTGGCC 
TATGTCCGTA AAGTGCTGCA GATTATACCT CAGACTATGT TTAGTCTCCT GTCTCAGATC 
ATTCAATTGC AAACACATCA CATCAAGGAA GTACCCACCA GACTAGAGAA GGAGAAATTG 
CGTGACTTTG CCCAGCTTGA TATGAGATAT GATGTGGCCA GACTTACTCA CTCTATCTCT 
GTCTTCACTG AGGGCATCTT AATGATGAAG ACAACATTGG TTGGGATTAT TAAGGTCGAT 
CCAAAACAAC TCTTGGAAGA TGGAATCAGG AAGGAGTTGG TACAGCAAGT GGCTAGTGCT 
TTACATCATG GCATTATGTT CAACCCAAAA GCCAAGACAA GTGAGTTGAT GCAACGACTT 
GATGCTCTAG GAGCTAAGAT GGATGGATTT GTTCGCTCTT TTGAGTACAT CCAAGACTAT 
GTCAACATCC ATGGTTTGAA GATTTGGCAA GAAGAAGTGT CTCGCATCAT CAATTACAAC 
ATGGAACAAG AATGCAACAG CTTCCTGAGG ACTAAGGTCC ATGATTGGCA AAGCATATAC 
CAGTCACCAA CCATCCCAAT TCCAAAGTTC CAACCAGTGG ACGGTTCCAT TAACTTCATG 
GGGAGGTTGG TCAGGGAAAT TCTCAGAATT ACTGATCCCA GATCCACTTC CTACATTGAC 
CAGATGAAAA CCTGGTACGA TGTCAAGACT AAACAAGAAG TGCTGGACAT CAAAGTCTTC 
TCCAAAGTCA GACAAAGCAT CGGTACCTGT GGTCTGACTG GTTTGGATCG ACTGATGTGC 
TTCATGATGG TCAGGGAACT TCAACAATTC CTAGGATACA TCGAGCATGG TGTCCTTCGT 
GAGAAAGCTT GGCTAGAAAT GTTTGGTTCA CTCATGAAGA CTCTCAACCC AGTCAGAGGA 
CTCATCAGTA ACCCTAACAA GGTGTACAGC CACTATATCA CCAGAGCAAA CAAGATGTGG 
TCACCATTCG TCAACCTTGT TCTCAAGCTT GGCCAGATGC AGTTGCTTCG CAGGCAGATT 
GCAAATGAAC TCAATTTTGC CTGCAAGTTT GAGTCAAAGT TCCTTGCCGG TGCATTGGAA 
ACCATGAACT CCGGTTTGCT AGCTGATGTT GAGGCCCACT ATCAAGATCC CTCTAGACCC 
TACCCAAAGG ATGACAATCC TCTCATGTTT GAGTTAGCAT CATATCTTGA ATCAGCTGGA 
ATCAACAACC CACTTTCCAA GATCTATGTA ACTACCAAGA GACTTCCATA TTTCTCTCTC 
TTCACATTCC TGTTTGTTGT CTCCCAGCTT CCAAAGTTTC AGTACCTCAA GAGTGTTGGA 
AGTATGGTGG GTAAGCTTCC CAAAGATCCT CTGGATGGTC CCCCACTTGT TGTTGGTACC 
CTGACTCTTC TGAGACAGTT CCATTCAGAG ACCACAGACC AGTTCCTGGC ATTGCTTGGA 
CAGTATGTCA GATCATCTGT TGAGAGCACT GCTGCAGGGG GCAAAGCATC TGAGTTAACA 
CCTGAGGTTG TTAATGTCCT GTCTTTCCTT GACGACTTCA CATACTTTGC TGGATTGTCT 
AGGCAGAGTG TAGAGTCACA TATTCCAGCT TACATCTTTG ATGAGTTCAA GAGCACTTGC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022102608.1
CDS	378..3863
Translation	MADFLADNNLCGQTLLKLVSRGNAIIAELLRLADFIPPVFKLDNKFDQMKYGDILFDFNYFKGSEYYDNKIDSRPELQDLDEIFRENHIDILRRFYLAFESIHKYVTDLNRFLEDLEEGVYIQQTLETVLLNEDGKQLLCESLYLYGVMLLVVDMRIDGPVRERMLVSYHRYSAAKAAVDSNMDDVCKLLRSTGFYSAPGAKRPLNYPENYFKRIPIPPHFIEMVIARLRSDDIYNQISAYPLPEHRSTALATQAAMLYISLYFSPDTLHGQQAKMREIVDKHFPDNWMISIYMGIVINLADMWEPYKAARTALNNTLDLTNVKTLTVKHNQKVDQLIKLLRQYLTEGTLTEEYVLDNIPKLMNCLRECNVNLRWLMLHTADGPWDGNKKIRQMKEAMILESKFSPRTLFELLLDSAQFEFKLKEMFKKMLSEKQKKWELYKQESVDRMTELGEVFSGTKPLTRVAKNENLQAWFAEMAKQINSLNYDDSTAAGRKIVQLIQALEEVQEFHQLESNLQVRQFLLDTRQFLHQMIRTINIKEEVLITMQIVADLSYAWELIDTYTPYMQQGIKSNPGLVTKLRATFLKLASALDLPLIRINQANSPDLISVSQYYSSELVAYVRKVLQIIPQTMFSLLSQIIQLQTHHIKEVPTRLEKEKLRDFAQLDMRYDVARLTHSISVFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVQQVASALHHGIMFNPKAKTSELMQRLDALGAKMDGFVRSFEYIQDYVNIHGLKIWQEEVSRIINYNMEQECNSFLRTKVHDWQSIYQSPTIPIPKFQPVDGSINFMGRLVREILRITDPRSTSYIDQMKTWYDVKTKQEVLDIKVFSKVRQSIGTCGLTGLDRLMCFMMVRELQQFLGYIEHGVLREKAWLEMFGSLMKTLNPVRGLISNPNKVYSHYITRANKMWSPFVNLVLKLGQMQLLRRQIANELNFACKFESKFLAGALETMNSGLLADVEAHYQDPSRPYPKDDNPLMFELASYLESAGINNPLSKIYVTTKRLPYFSLFTFLFVVSQLPKFQYLKSVGSMVGKLPKDPLDGPPLVVGTLTLLRQFHSETTDQFLALLGQYVRSSVESTAAGGKASELTPEVVNVLSFLDDFTYFAGLSRQSVESHIPAYIFDEFKSTCQ

Target ORF information:

RefSeq Version	XM_022246916.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci WASH complex subunit 5-like (LOC110985703), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022246916.1

ORF Insert Sequence:

ATGGCTGATT TTCTGGCAGA CAACAACCTT TGTGGACAAA CTCTACTCAA GTTGGTCTCT 
CGTGGCAATG CTATCATTGC TGAGCTACTT CGTTTGGCTG ACTTTATCCC TCCTGTCTTC 
AAGCTTGATA ATAAGTTTGA TCAGATGAAG TATGGGGACA TTCTCTTTGA TTTCAATTAC 
TTCAAGGGAT CAGAATACTA CGATAACAAG ATTGATTCCA GGCCTGAGCT GCAAGACCTT 
GATGAAATCT TCCGGGAAAA TCATATTGAC ATTTTACGGC GCTTCTATCT TGCCTTTGAG 
AGTATCCATA AATATGTGAC TGATCTCAAT CGATTTCTTG AAGATTTGGA GGAAGGGGTT 
TACATTCAGC AAACACTAGA GACTGTGCTA CTCAATGAAG ACGGCAAGCA ACTTCTGTGT 
GAATCCTTGT ATCTATACGG TGTGATGCTG CTTGTGGTTG ATATGAGAAT TGATGGCCCA 
GTCAGAGAAC GAATGTTGGT ATCTTATCAC AGATACAGTG CTGCCAAGGC TGCTGTTGAT 
TCCAACATGG ACGATGTCTG CAAACTATTG CGATCAACTG GATTCTATTC AGCTCCAGGA 
GCAAAAAGAC CTCTTAACTA CCCAGAGAAC TACTTCAAGC GCATTCCGAT ACCCCCTCAT 
TTCATTGAGA TGGTCATTGC CAGGCTACGT TCAGATGACA TATACAACCA GATCTCAGCC 
TATCCCTTAC CAGAGCACCG AAGCACAGCG TTAGCCACAC AAGCAGCCAT GTTATACATC 
AGTCTCTACT TCTCTCCAGA CACACTGCAC GGTCAACAGG CTAAGATGAG GGAAATTGTT 
GACAAACATT TTCCAGATAA TTGGATGATC AGCATTTACA TGGGAATTGT GATCAATCTG 
GCAGACATGT GGGAACCATA CAAAGCAGCA CGAACAGCTC TTAATAACAC CCTGGACTTG 
ACCAATGTCA AGACCTTGAC CGTGAAGCAT AACCAGAAAG TTGACCAGTT GATCAAGCTG 
TTACGTCAGT ATTTGACAGA AGGAACACTT ACAGAGGAAT ATGTCCTGGA CAACATTCCT 
AAGCTGATGA ACTGTCTAAG AGAATGTAAC GTCAATCTGC GCTGGCTTAT GCTACACACT 
GCAGATGGGC CCTGGGATGG TAACAAGAAG ATAAGACAGA TGAAGGAAGC CATGATTTTG 
GAATCCAAGT TTAGTCCTCG GACTTTATTT GAGCTTCTGT TGGATTCAGC ACAATTTGAG 
TTCAAACTCA AGGAGATGTT CAAGAAGATG TTGTCAGAAA AGCAAAAGAA GTGGGAACTG 
TACAAACAAG AGAGTGTAGA TCGAATGACT GAGCTTGGTG AGGTCTTCTC AGGAACCAAA 
CCATTGACTC GCGTTGCCAA GAATGAGAAC CTTCAGGCTT GGTTTGCTGA GATGGCTAAA 
CAGATAAACT CACTCAACTA CGATGACTCT ACTGCTGCAG GTCGCAAGAT TGTCCAACTC 
ATACAGGCCC TGGAAGAGGT GCAAGAGTTT CATCAGTTGG AGAGCAACTT GCAAGTTCGT 
CAGTTTCTGC TGGACACTCG CCAGTTCCTT CATCAAATGA TTCGAACCAT TAACATCAAG 
GAAGAGGTGC TCATCACAAT GCAGATTGTA GCTGATCTGT CTTATGCCTG GGAACTCATT 
GATACGTACA CACCTTACAT GCAGCAGGGC ATCAAAAGCA ACCCAGGTCT GGTTACCAAA 
CTGAGAGCAA CATTCCTGAA GCTGGCATCA GCTCTTGATC TGCCTCTGAT ACGAATCAAT 
CAAGCCAACA GTCCTGACCT GATAAGTGTC TCTCAATACT ACTCCAGTGA GCTTGTGGCC 
TATGTCCGTA AAGTGCTGCA GATTATACCT CAGACTATGT TTAGTCTCCT GTCTCAGATC 
ATTCAATTGC AAACACATCA CATCAAGGAA GTACCCACCA GACTAGAGAA GGAGAAATTG 
CGTGACTTTG CCCAGCTTGA TATGAGATAT GATGTGGCCA GACTTACTCA CTCTATCTCT 
GTCTTCACTG AGGGCATCTT AATGATGAAG ACAACATTGG TTGGGATTAT TAAGGTCGAT 
CCAAAACAAC TCTTGGAAGA TGGAATCAGG AAGGAGTTGG TACAGCAAGT GGCTAGTGCT 
TTACATCATG GCATTATGTT CAACCCAAAA GCCAAGACAA GTGAGTTGAT GCAACGACTT 
GATGCTCTAG GAGCTAAGAT GGATGGATTT GTTCGCTCTT TTGAGTACAT CCAAGACTAT 
GTCAACATCC ATGGTTTGAA GATTTGGCAA GAAGAAGTGT CTCGCATCAT CAATTACAAC 
ATGGAACAAG AATGCAACAG CTTCCTGAGG ACTAAGGTCC ATGATTGGCA AAGCATATAC 
CAGTCACCAA CCATCCCAAT TCCAAAGTTC CAACCAGTGG ACGGTTCCAT TAACTTCATG 
GGGAGGTTGG TCAGGGAAAT TCTCAGAATT ACTGATCCCA GATCCACTTC CTACATTGAC 
CAGATGAAAA CCTGGTACGA TGTCAAGACT AAACAAGAAG TGCTGGACAT CAAAGTCTTC 
TCCAAAGTCA GACAAAGCAT CGGTACCTGT GGTCTGACTG GTTTGGATCG ACTGATGTGC 
TTCATGATGG TCAGGGAACT TCAACAATTC CTAGGATACA TCGAGCATGG TGTCCTTCGT 
GAGAAAGCTT GGCTAGAAAT GTTTGGTTCA CTCATGAAGA CTCTCAACCC AGTCAGAGGA 
CTCATCAGTA ACCCTAACAA GGTGTACAGC CACTATATCA CCAGAGCAAA CAAGATGTGG 
TCACCATTCG TCAACCTTGT TCTCAAGCTT GGCCAGATGC AGTTGCTTCG CAGGCAGATT 
GCAAATGAAC TCAATTTTGC CTGCAAGTTT GAGTCAAAGT TCCTTGCCGG TGCATTGGAA 
ACCATGAACT CCGGTTTGCT AGCTGATGTT GAGGCCCACT ATCAAGATCC CTCTAGACCC 
TACCCAAAGG ATGACAATCC TCTCATGTTT GAGTTAGCAT CATATCTTGA ATCAGCTGGA 
ATCAACAACC CACTTTCCAA GATCTATGTA ACTACCAAGA GACTTCCATA TTTCTCTCTC 
TTCACATTCC TGTTTGTTGT CTCCCAGCTT CCAAAGTTTC AGTACCTCAA GAGTGTTGGA 
AGTATGGTGG GTAAGCTTCC CAAAGATCCT CTGGATGGTC CCCCACTTGT TGTTGGTACC 
CTGACTCTTC TGAGACAGTT CCATTCAGAG ACCACAGACC AGTTCCTGGC ATTGCTTGGA 
CAGTATGTCA GATCATCTGT TGAGAGCACT GCTGCAGGGG GCAAAGCATC TGAGTTAACA 
CCTGAGGTTG TTAATGTCCT GTCTTTCCTT GACGACTTCA CATACTTTGC TGGATTGTCT 
AGGCAGAGTG TAGAGTCACA TATTCCAGCT TACATCTTTG ATGAGTTCAA GAGCACTTGC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110985703 ( XM_022246917.1 ) cDNA ORF clone, Acanthaster planci(crown-of-thorns starfish) -> XP_022102609.1 Acanthaster planci WASH complex subunit 5-like (LOC110985703), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAc167947
Clone ID Related Accession (Same CDS sequence)	XM_022246916.1 , XM_022246917.1
Accession Version	XM_022246917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-07
Organism	Acanthaster planci(crown-of-thorns starfish)
Product	WASH complex subunit 5-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019091500.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acanthaster planci Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATT TTCTGGCAGA CAACAACCTT TGTGGACAAA CTCTACTCAA GTTGGTCTCT 
CGTGGCAATG CTATCATTGC TGAGCTACTT CGTTTGGCTG ACTTTATCCC TCCTGTCTTC 
AAGCTTGATA ATAAGTTTGA TCAGATGAAG TATGGGGACA TTCTCTTTGA TTTCAATTAC 
TTCAAGGGAT CAGAATACTA CGATAACAAG ATTGATTCCA GGCCTGAGCT GCAAGACCTT 
GATGAAATCT TCCGGGAAAA TCATATTGAC ATTTTACGGC GCTTCTATCT TGCCTTTGAG 
AGTATCCATA AATATGTGAC TGATCTCAAT CGATTTCTTG AAGATTTGGA GGAAGGGGTT 
TACATTCAGC AAACACTAGA GACTGTGCTA CTCAATGAAG ACGGCAAGCA ACTTCTGTGT 
GAATCCTTGT ATCTATACGG TGTGATGCTG CTTGTGGTTG ATATGAGAAT TGATGGCCCA 
GTCAGAGAAC GAATGTTGGT ATCTTATCAC AGATACAGTG CTGCCAAGGC TGCTGTTGAT 
TCCAACATGG ACGATGTCTG CAAACTATTG CGATCAACTG GATTCTATTC AGCTCCAGGA 
GCAAAAAGAC CTCTTAACTA CCCAGAGAAC TACTTCAAGC GCATTCCGAT ACCCCCTCAT 
TTCATTGAGA TGGTCATTGC CAGGCTACGT TCAGATGACA TATACAACCA GATCTCAGCC 
TATCCCTTAC CAGAGCACCG AAGCACAGCG TTAGCCACAC AAGCAGCCAT GTTATACATC 
AGTCTCTACT TCTCTCCAGA CACACTGCAC GGTCAACAGG CTAAGATGAG GGAAATTGTT 
GACAAACATT TTCCAGATAA TTGGATGATC AGCATTTACA TGGGAATTGT GATCAATCTG 
GCAGACATGT GGGAACCATA CAAAGCAGCA CGAACAGCTC TTAATAACAC CCTGGACTTG 
ACCAATGTCA AGACCTTGAC CGTGAAGCAT AACCAGAAAG TTGACCAGTT GATCAAGCTG 
TTACGTCAGT ATTTGACAGA AGGAACACTT ACAGAGGAAT ATGTCCTGGA CAACATTCCT 
AAGCTGATGA ACTGTCTAAG AGAATGTAAC GTCAATCTGC GCTGGCTTAT GCTACACACT 
GCAGATGGGC CCTGGGATGG TAACAAGAAG ATAAGACAGA TGAAGGAAGC CATGATTTTG 
GAATCCAAGT TTAGTCCTCG GACTTTATTT GAGCTTCTGT TGGATTCAGC ACAATTTGAG 
TTCAAACTCA AGGAGATGTT CAAGAAGATG TTGTCAGAAA AGCAAAAGAA GTGGGAACTG 
TACAAACAAG AGAGTGTAGA TCGAATGACT GAGCTTGGTG AGGTCTTCTC AGGAACCAAA 
CCATTGACTC GCGTTGCCAA GAATGAGAAC CTTCAGGCTT GGTTTGCTGA GATGGCTAAA 
CAGATAAACT CACTCAACTA CGATGACTCT ACTGCTGCAG GTCGCAAGAT TGTCCAACTC 
ATACAGGCCC TGGAAGAGGT GCAAGAGTTT CATCAGTTGG AGAGCAACTT GCAAGTTCGT 
CAGTTTCTGC TGGACACTCG CCAGTTCCTT CATCAAATGA TTCGAACCAT TAACATCAAG 
GAAGAGGTGC TCATCACAAT GCAGATTGTA GCTGATCTGT CTTATGCCTG GGAACTCATT 
GATACGTACA CACCTTACAT GCAGCAGGGC ATCAAAAGCA ACCCAGGTCT GGTTACCAAA 
CTGAGAGCAA CATTCCTGAA GCTGGCATCA GCTCTTGATC TGCCTCTGAT ACGAATCAAT 
CAAGCCAACA GTCCTGACCT GATAAGTGTC TCTCAATACT ACTCCAGTGA GCTTGTGGCC 
TATGTCCGTA AAGTGCTGCA GATTATACCT CAGACTATGT TTAGTCTCCT GTCTCAGATC 
ATTCAATTGC AAACACATCA CATCAAGGAA GTACCCACCA GACTAGAGAA GGAGAAATTG 
CGTGACTTTG CCCAGCTTGA TATGAGATAT GATGTGGCCA GACTTACTCA CTCTATCTCT 
GTCTTCACTG AGGGCATCTT AATGATGAAG ACAACATTGG TTGGGATTAT TAAGGTCGAT 
CCAAAACAAC TCTTGGAAGA TGGAATCAGG AAGGAGTTGG TACAGCAAGT GGCTAGTGCT 
TTACATCATG GCATTATGTT CAACCCAAAA GCCAAGACAA GTGAGTTGAT GCAACGACTT 
GATGCTCTAG GAGCTAAGAT GGATGGATTT GTTCGCTCTT TTGAGTACAT CCAAGACTAT 
GTCAACATCC ATGGTTTGAA GATTTGGCAA GAAGAAGTGT CTCGCATCAT CAATTACAAC 
ATGGAACAAG AATGCAACAG CTTCCTGAGG ACTAAGGTCC ATGATTGGCA AAGCATATAC 
CAGTCACCAA CCATCCCAAT TCCAAAGTTC CAACCAGTGG ACGGTTCCAT TAACTTCATG 
GGGAGGTTGG TCAGGGAAAT TCTCAGAATT ACTGATCCCA GATCCACTTC CTACATTGAC 
CAGATGAAAA CCTGGTACGA TGTCAAGACT AAACAAGAAG TGCTGGACAT CAAAGTCTTC 
TCCAAAGTCA GACAAAGCAT CGGTACCTGT GGTCTGACTG GTTTGGATCG ACTGATGTGC 
TTCATGATGG TCAGGGAACT TCAACAATTC CTAGGATACA TCGAGCATGG TGTCCTTCGT 
GAGAAAGCTT GGCTAGAAAT GTTTGGTTCA CTCATGAAGA CTCTCAACCC AGTCAGAGGA 
CTCATCAGTA ACCCTAACAA GGTGTACAGC CACTATATCA CCAGAGCAAA CAAGATGTGG 
TCACCATTCG TCAACCTTGT TCTCAAGCTT GGCCAGATGC AGTTGCTTCG CAGGCAGATT 
GCAAATGAAC TCAATTTTGC CTGCAAGTTT GAGTCAAAGT TCCTTGCCGG TGCATTGGAA 
ACCATGAACT CCGGTTTGCT AGCTGATGTT GAGGCCCACT ATCAAGATCC CTCTAGACCC 
TACCCAAAGG ATGACAATCC TCTCATGTTT GAGTTAGCAT CATATCTTGA ATCAGCTGGA 
ATCAACAACC CACTTTCCAA GATCTATGTA ACTACCAAGA GACTTCCATA TTTCTCTCTC 
TTCACATTCC TGTTTGTTGT CTCCCAGCTT CCAAAGTTTC AGTACCTCAA GAGTGTTGGA 
AGTATGGTGG GTAAGCTTCC CAAAGATCCT CTGGATGGTC CCCCACTTGT TGTTGGTACC 
CTGACTCTTC TGAGACAGTT CCATTCAGAG ACCACAGACC AGTTCCTGGC ATTGCTTGGA 
CAGTATGTCA GATCATCTGT TGAGAGCACT GCTGCAGGGG GCAAAGCATC TGAGTTAACA 
CCTGAGGTTG TTAATGTCCT GTCTTTCCTT GACGACTTCA CATACTTTGC TGGATTGTCT 
AGGCAGAGTG TAGAGTCACA TATTCCAGCT TACATCTTTG ATGAGTTCAA GAGCACTTGC 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022102609.1
CDS	130..3615
Translation	MADFLADNNLCGQTLLKLVSRGNAIIAELLRLADFIPPVFKLDNKFDQMKYGDILFDFNYFKGSEYYDNKIDSRPELQDLDEIFRENHIDILRRFYLAFESIHKYVTDLNRFLEDLEEGVYIQQTLETVLLNEDGKQLLCESLYLYGVMLLVVDMRIDGPVRERMLVSYHRYSAAKAAVDSNMDDVCKLLRSTGFYSAPGAKRPLNYPENYFKRIPIPPHFIEMVIARLRSDDIYNQISAYPLPEHRSTALATQAAMLYISLYFSPDTLHGQQAKMREIVDKHFPDNWMISIYMGIVINLADMWEPYKAARTALNNTLDLTNVKTLTVKHNQKVDQLIKLLRQYLTEGTLTEEYVLDNIPKLMNCLRECNVNLRWLMLHTADGPWDGNKKIRQMKEAMILESKFSPRTLFELLLDSAQFEFKLKEMFKKMLSEKQKKWELYKQESVDRMTELGEVFSGTKPLTRVAKNENLQAWFAEMAKQINSLNYDDSTAAGRKIVQLIQALEEVQEFHQLESNLQVRQFLLDTRQFLHQMIRTINIKEEVLITMQIVADLSYAWELIDTYTPYMQQGIKSNPGLVTKLRATFLKLASALDLPLIRINQANSPDLISVSQYYSSELVAYVRKVLQIIPQTMFSLLSQIIQLQTHHIKEVPTRLEKEKLRDFAQLDMRYDVARLTHSISVFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVQQVASALHHGIMFNPKAKTSELMQRLDALGAKMDGFVRSFEYIQDYVNIHGLKIWQEEVSRIINYNMEQECNSFLRTKVHDWQSIYQSPTIPIPKFQPVDGSINFMGRLVREILRITDPRSTSYIDQMKTWYDVKTKQEVLDIKVFSKVRQSIGTCGLTGLDRLMCFMMVRELQQFLGYIEHGVLREKAWLEMFGSLMKTLNPVRGLISNPNKVYSHYITRANKMWSPFVNLVLKLGQMQLLRRQIANELNFACKFESKFLAGALETMNSGLLADVEAHYQDPSRPYPKDDNPLMFELASYLESAGINNPLSKIYVTTKRLPYFSLFTFLFVVSQLPKFQYLKSVGSMVGKLPKDPLDGPPLVVGTLTLLRQFHSETTDQFLALLGQYVRSSVESTAAGGKASELTPEVVNVLSFLDDFTYFAGLSRQSVESHIPAYIFDEFKSTCQ

Target ORF information:

RefSeq Version	XM_022246917.1
Organism	Acanthaster planci(crown-of-thorns starfish)
Definition	Acanthaster planci WASH complex subunit 5-like (LOC110985703), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022246917.1

ORF Insert Sequence:

ATGGCTGATT TTCTGGCAGA CAACAACCTT TGTGGACAAA CTCTACTCAA GTTGGTCTCT 
CGTGGCAATG CTATCATTGC TGAGCTACTT CGTTTGGCTG ACTTTATCCC TCCTGTCTTC 
AAGCTTGATA ATAAGTTTGA TCAGATGAAG TATGGGGACA TTCTCTTTGA TTTCAATTAC 
TTCAAGGGAT CAGAATACTA CGATAACAAG ATTGATTCCA GGCCTGAGCT GCAAGACCTT 
GATGAAATCT TCCGGGAAAA TCATATTGAC ATTTTACGGC GCTTCTATCT TGCCTTTGAG 
AGTATCCATA AATATGTGAC TGATCTCAAT CGATTTCTTG AAGATTTGGA GGAAGGGGTT 
TACATTCAGC AAACACTAGA GACTGTGCTA CTCAATGAAG ACGGCAAGCA ACTTCTGTGT 
GAATCCTTGT ATCTATACGG TGTGATGCTG CTTGTGGTTG ATATGAGAAT TGATGGCCCA 
GTCAGAGAAC GAATGTTGGT ATCTTATCAC AGATACAGTG CTGCCAAGGC TGCTGTTGAT 
TCCAACATGG ACGATGTCTG CAAACTATTG CGATCAACTG GATTCTATTC AGCTCCAGGA 
GCAAAAAGAC CTCTTAACTA CCCAGAGAAC TACTTCAAGC GCATTCCGAT ACCCCCTCAT 
TTCATTGAGA TGGTCATTGC CAGGCTACGT TCAGATGACA TATACAACCA GATCTCAGCC 
TATCCCTTAC CAGAGCACCG AAGCACAGCG TTAGCCACAC AAGCAGCCAT GTTATACATC 
AGTCTCTACT TCTCTCCAGA CACACTGCAC GGTCAACAGG CTAAGATGAG GGAAATTGTT 
GACAAACATT TTCCAGATAA TTGGATGATC AGCATTTACA TGGGAATTGT GATCAATCTG 
GCAGACATGT GGGAACCATA CAAAGCAGCA CGAACAGCTC TTAATAACAC CCTGGACTTG 
ACCAATGTCA AGACCTTGAC CGTGAAGCAT AACCAGAAAG TTGACCAGTT GATCAAGCTG 
TTACGTCAGT ATTTGACAGA AGGAACACTT ACAGAGGAAT ATGTCCTGGA CAACATTCCT 
AAGCTGATGA ACTGTCTAAG AGAATGTAAC GTCAATCTGC GCTGGCTTAT GCTACACACT 
GCAGATGGGC CCTGGGATGG TAACAAGAAG ATAAGACAGA TGAAGGAAGC CATGATTTTG 
GAATCCAAGT TTAGTCCTCG GACTTTATTT GAGCTTCTGT TGGATTCAGC ACAATTTGAG 
TTCAAACTCA AGGAGATGTT CAAGAAGATG TTGTCAGAAA AGCAAAAGAA GTGGGAACTG 
TACAAACAAG AGAGTGTAGA TCGAATGACT GAGCTTGGTG AGGTCTTCTC AGGAACCAAA 
CCATTGACTC GCGTTGCCAA GAATGAGAAC CTTCAGGCTT GGTTTGCTGA GATGGCTAAA 
CAGATAAACT CACTCAACTA CGATGACTCT ACTGCTGCAG GTCGCAAGAT TGTCCAACTC 
ATACAGGCCC TGGAAGAGGT GCAAGAGTTT CATCAGTTGG AGAGCAACTT GCAAGTTCGT 
CAGTTTCTGC TGGACACTCG CCAGTTCCTT CATCAAATGA TTCGAACCAT TAACATCAAG 
GAAGAGGTGC TCATCACAAT GCAGATTGTA GCTGATCTGT CTTATGCCTG GGAACTCATT 
GATACGTACA CACCTTACAT GCAGCAGGGC ATCAAAAGCA ACCCAGGTCT GGTTACCAAA 
CTGAGAGCAA CATTCCTGAA GCTGGCATCA GCTCTTGATC TGCCTCTGAT ACGAATCAAT 
CAAGCCAACA GTCCTGACCT GATAAGTGTC TCTCAATACT ACTCCAGTGA GCTTGTGGCC 
TATGTCCGTA AAGTGCTGCA GATTATACCT CAGACTATGT TTAGTCTCCT GTCTCAGATC 
ATTCAATTGC AAACACATCA CATCAAGGAA GTACCCACCA GACTAGAGAA GGAGAAATTG 
CGTGACTTTG CCCAGCTTGA TATGAGATAT GATGTGGCCA GACTTACTCA CTCTATCTCT 
GTCTTCACTG AGGGCATCTT AATGATGAAG ACAACATTGG TTGGGATTAT TAAGGTCGAT 
CCAAAACAAC TCTTGGAAGA TGGAATCAGG AAGGAGTTGG TACAGCAAGT GGCTAGTGCT 
TTACATCATG GCATTATGTT CAACCCAAAA GCCAAGACAA GTGAGTTGAT GCAACGACTT 
GATGCTCTAG GAGCTAAGAT GGATGGATTT GTTCGCTCTT TTGAGTACAT CCAAGACTAT 
GTCAACATCC ATGGTTTGAA GATTTGGCAA GAAGAAGTGT CTCGCATCAT CAATTACAAC 
ATGGAACAAG AATGCAACAG CTTCCTGAGG ACTAAGGTCC ATGATTGGCA AAGCATATAC 
CAGTCACCAA CCATCCCAAT TCCAAAGTTC CAACCAGTGG ACGGTTCCAT TAACTTCATG 
GGGAGGTTGG TCAGGGAAAT TCTCAGAATT ACTGATCCCA GATCCACTTC CTACATTGAC 
CAGATGAAAA CCTGGTACGA TGTCAAGACT AAACAAGAAG TGCTGGACAT CAAAGTCTTC 
TCCAAAGTCA GACAAAGCAT CGGTACCTGT GGTCTGACTG GTTTGGATCG ACTGATGTGC 
TTCATGATGG TCAGGGAACT TCAACAATTC CTAGGATACA TCGAGCATGG TGTCCTTCGT 
GAGAAAGCTT GGCTAGAAAT GTTTGGTTCA CTCATGAAGA CTCTCAACCC AGTCAGAGGA 
CTCATCAGTA ACCCTAACAA GGTGTACAGC CACTATATCA CCAGAGCAAA CAAGATGTGG 
TCACCATTCG TCAACCTTGT TCTCAAGCTT GGCCAGATGC AGTTGCTTCG CAGGCAGATT 
GCAAATGAAC TCAATTTTGC CTGCAAGTTT GAGTCAAAGT TCCTTGCCGG TGCATTGGAA 
ACCATGAACT CCGGTTTGCT AGCTGATGTT GAGGCCCACT ATCAAGATCC CTCTAGACCC 
TACCCAAAGG ATGACAATCC TCTCATGTTT GAGTTAGCAT CATATCTTGA ATCAGCTGGA 
ATCAACAACC CACTTTCCAA GATCTATGTA ACTACCAAGA GACTTCCATA TTTCTCTCTC 
TTCACATTCC TGTTTGTTGT CTCCCAGCTT CCAAAGTTTC AGTACCTCAA GAGTGTTGGA 
AGTATGGTGG GTAAGCTTCC CAAAGATCCT CTGGATGGTC CCCCACTTGT TGTTGGTACC 
CTGACTCTTC TGAGACAGTT CCATTCAGAG ACCACAGACC AGTTCCTGGC ATTGCTTGGA 
CAGTATGTCA GATCATCTGT TGAGAGCACT GCTGCAGGGG GCAAAGCATC TGAGTTAACA 
CCTGAGGTTG TTAATGTCCT GTCTTTCCTT GACGACTTCA CATACTTTGC TGGATTGTCT 
AGGCAGAGTG TAGAGTCACA TATTCCAGCT TACATCTTTG ATGAGTTCAA GAGCACTTGC 
CAGTAG