ACA1_142240 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene
Clones
gRNAs

Summary

Gene Symbol	ACA1_142240
Entrez Gene ID	14923475
Full Name	actin subfamily protein
Gene Type	protein-coding
Organism	Acanthamoeba castellanii str. Neff

mRNA and Protein(s)

mRNA	Protein	Name
XM_004349571.1	XP_004349621.1	actin subfamily protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Clarke M, Lohan AJ, Liu B, Lagkouvardos I, Roy S, Zafar N, Bertelli C, Schilde C, Kianianmomeni A, B?rglin TR, Frech C, Turcotte B, Kopec KO, Synnott JM, Choo C, Paponov I, Finkler A, Heng Tan CS, Hutchins AP, Weinmeier T, Rattei T, Chu JS, Gimenez G, Irimia M, Rigden DJ, Fitzpatrick DA, Lorenzo-Morales J, Bateman A, Chiu CH, Tang P, Hegemann P, Fromm H, Raoult D, Greub G, Miranda-Saavedra D, Chen N, Nash P, Ginger ML, Horn M, Schaap P, Caler L, Loftus BJ
Genome biology14(2)R11(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACA1_142240 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACA1_142240 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc111889	XM_004349571.1 Latest version!	Acanthamoeba castellanii str. Neff actin subfamily protein (ACA1_142240) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACA1_142240 ( XM_004349571.1 ) cDNA ORF clone, Acanthamoeba castellanii str. Neff -> XP_004349621.1 Acanthamoeba castellanii str. Neff actin subfamily protein (ACA1_142240) mRNA, complete cds.

Price & Availability↑

CloneID	OAc111889
Clone ID Related Accession (Same CDS sequence)	XM_004349571.1
Accession Version	XM_004349571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2520bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-03-26
Organism	Acanthamoeba castellanii str. Neff
Product	actin subfamily protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_004457620). Long-term curation of the sequence and subsequent annotation updates will be the responsibility of the EuPathDB Bioinformatics Resource Center http://EuPathDB.org. Annotation can be viewed at http://AmoebaDB.org and inquires can be sent to [email protected]. COMPLETENESS: incomplete on both ends.

ATGACGAAGG CGCCCACCAC AGGACCGAGA CGCGTCGACC TGGGCGATGT TGGGGCGCCC 
AATGCCGACC GCATAGAGGC GTGGGTGGTG GACGAGGTGC TCACAGCCGA CGAGTGCAGC 
CACTACATCG ACCAGGCCGA GCGCGTGGGC CTCGCCAGTC TCGAGACCGT CTTCCCGCAG 
GCTTACCGCA GCTCCGACCG TCTCCTCACC CTCGCCCCAC CGCACATTGT ACGCGGCCTC 
TTCGCCCGGC TCCTGCCCGC CCTCGATCGC CGTGATTTGC TGTGGAATCC GATGGGGCCA 
GGCACGGAAG GCGTGTGGTT GCCGTGCAGC CTCAACGAAT GCTTCAAGAT CGGCCGCTAT 
CGCCCCGGGT GCCACTTCAG CGCGCACATG GACGGGCCGT GGGTGCCGAG CGCACACGAG 
GCCAGCATCT TCACCGTGCT CATCTACCTC AACGACGCCA CCACGTCGCC CGCCTGCCAC 
GGCGGACAGA CTAACTTCCT CGCAGCTGCC GCCGCCCACG CACCTGATGG CGCCAGCCCG 
CATGCGGCGC CACGTGACGA CGACCGGGTG GGGGCTCGGC TGGCGGAGGG GGTGAAGTAC 
GTGCTGCGCA CGGAGCTCAT CTTCAGGCGC GTCGACTCTC CGCTCACGCC GCGACACGTG 
TCGTCGGCTG ACGAGTCGGC GTGGCGCCGC ATCGTCGCCC TCTACGACCA ATCCGAGGAG 
CTGTTGCGAG AGGGCGACAC GGAGGGCTTT GTCGAGGCCT ATCTCGCCGC CATCGAGCTC 
CAGCAGCAGC GGACCAGCGA CAATGTCCAT CACCGCGTCG GCAATGGTGT CGACGGCGAT 
GACGTCGCCG CGCGCGGCTT GTTCACGGCG CTGCCGGCCG AGGTCGTGTG GCGCGTGACG 
AGCCGCCTGG GCGCGGGCGA TGTCGCGCGG CTGATGTGCG CCGCCGACAA GCGGCTCTAC 
CGCCTCTTGC GCGTCGATTC CTCGTGGCGC GCCTTCTATT TCCGTCGACT CGGGCTCAAC 
TACAGCGAGC GCATCCACCG CCGCGCTCTG CATGACGACT CTGACACCGC AGAGGAGCTG 
GAGGACGAGG CGATGCAGCT GCGCTTCGAC TGGTACGACC TGTGCCGCCG CCACAGCCGC 
AGACGCGGCG GACCCACCAC CTCAGGCGCG CCACGTGTCG CCACGTCAGC AGCGCCGATG 
ATGATGATGA AGAAGTGCGT CCAATCGGTC GACGACAGCG TGAAGCGGTC ACACGTGGCC 
GACGAGGCGC ACGATCTACA TCCCATGCGC CACGCAACTA TGCCGTCGTC GCCGATGTCG 
ACTAGTACGG TCCACCGTCG CGAGACCACC ACTACCACCG GCGGCGGGCT GCCGTTTCGG 
TGGATGCTGG GGGACGTCGG CGGGGACGTC GTGACGCCAC AGGCGGACTA CAGGCAGGAG 
GACGTCTCCT ATATCGTGTT GGACAATGGC GGCCACACCG TCAAAGCCGG GTTTGGAGGA 
GATGACGCGC CCCGGTCGCG CATTCCGGCG GTGGTGGGCT ACCCGCGACA CAGTCACTCG 
GTGCCGGTGG GCATGGGCAT CAAGTGGTTC TACGTGGGCG ACGAAGTCAT CGCCAAGCGG 
GGCATCCTCC GCCAAGAGAT CCCCATGCAC AATCGGACCA TCCTTAACTT CGACGCCATG 
GAACGCGTCT GGCACCATAC GTTCTACAAC GAGCTGCGGG TAGCGCCAGA GGAGCATCCG 
CTGGTGATGA GCCACACATC GTTCACGCCG CAGGCACACC TTGAAAAGAT GCTCCAGATC 
GCCATGGAGA CTTTCAACGT GCCCGCCTTC TTCTCCGCCA ACCAGCAAGT GTTGGCTAGC 
GTGTGGCTAG GGAAGAAGAC GGCCCTCGTG GTCGACATCG GTGTTCATGA CACCCACATC 
GTGCCCGTCG TTCAAGGAAG GGTGCTGGAG CAGGGCGTGG TGCGGCTGCC TTGCCTCGGC 
GGCGGGATGC TCACAGACCA CCTCCTCCAC CTCCTGACGC AGCAGCCCGG CAGCTCATTC 
GAGGAGAGCG GAGTCTACAG GGGCGTCGCC AATGAAATGA AGCACGCGTT GGGATTCTGT 
TCGCTGGCGC CAGAGCGTGA CCGCGAGGTT GCCGCGCGAC AGCTGCGCGA CGGCGCGCGC 
GAGCCGGTGC CGTTCTTCGA GTGTACGCGC GTGCGGGGCA CCTATCTCGA CGTGCTGCCC 
AGCGAGCTGC TGGTGCTGAT CGACAGGTCG GTGCAGCACC ACCGCATCGT TCGCCCCTAC 
TCCATGCCCG ACGAGAGCAA AGTGGTGGTG GGGAGTGAGC GGTTTGCCTG CGTGGAACCG 
CTCTTCCAAC CGCAGCTGCT CGACTGGGTG GTGGCCACCA AGCACAAGTT TATGTACCGG 
TGGTGTGACG AGGTGAACGA GGCGAGGAAG GGCATGCACG AGGGCCTGGC CCATAGCATC 
GACTCCTTCA AAGACGCCCC CGACCTCCAG TTAACCCACT CCCGCCTCCT TGTAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004349621.1
CDS	1..2520
Translation	MTKAPTTGPRRVDLGDVGAPNADRIEAWVVDEVLTADECSHYIDQAERVGLASLETVFPQAYRSSDRLLTLAPPHIVRGLFARLLPALDRRDLLWNPMGPGTEGVWLPCSLNECFKIGRYRPGCHFSAHMDGPWVPSAHEASIFTVLIYLNDATTSPACHGGQTNFLAAAAAHAPDGASPHAAPRDDDRVGARLAEGVKYVLRTELIFRRVDSPLTPRHVSSADESAWRRIVALYDQSEELLREGDTEGFVEAYLAAIELQQQRTSDNVHHRVGNGVDGDDVAARGLFTALPAEVVWRVTSRLGAGDVARLMCAADKRLYRLLRVDSSWRAFYFRRLGLNYSERIHRRALHDDSDTAEELEDEAMQLRFDWYDLCRRHSRRRGGPTTSGAPRVATSAAPMMMMKKCVQSVDDSVKRSHVADEAHDLHPMRHATMPSSPMSTSTVHRRETTTTTGGGLPFRWMLGDVGGDVVTPQADYRQEDVSYIVLDNGGHTVKAGFGGDDAPRSRIPAVVGYPRHSHSVPVGMGIKWFYVGDEVIAKRGILRQEIPMHNRTILNFDAMERVWHHTFYNELRVAPEEHPLVMSHTSFTPQAHLEKMLQIAMETFNVPAFFSANQQVLASVWLGKKTALVVDIGVHDTHIVPVVQGRVLEQGVVRLPCLGGGMLTDHLLHLLTQQPGSSFEESGVYRGVANEMKHALGFCSLAPERDREVAARQLRDGAREPVPFFECTRVRGTYLDVLPSELLVLIDRSVQHHRIVRPYSMPDESKVVVGSERFACVEPLFQPQLLDWVVATKHKFMYRWCDEVNEARKGMHEGLAHSIDSFKDAPDLQLTHSRLLVI

Target ORF information:

RefSeq Version	XM_004349571.1
Organism	Acanthamoeba castellanii str. Neff
Definition	Acanthamoeba castellanii str. Neff actin subfamily protein (ACA1_142240) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004349571.1

ORF Insert Sequence:

ATGACGAAGG CGCCCACCAC AGGACCGAGA CGCGTCGACC TGGGCGATGT TGGGGCGCCC 
AATGCCGACC GCATAGAGGC GTGGGTGGTG GACGAGGTGC TCACAGCCGA CGAGTGCAGC 
CACTACATCG ACCAGGCCGA GCGCGTGGGC CTCGCCAGTC TCGAGACCGT CTTCCCGCAG 
GCTTACCGCA GCTCCGACCG TCTCCTCACC CTCGCCCCAC CGCACATTGT ACGCGGCCTC 
TTCGCCCGGC TCCTGCCCGC CCTCGATCGC CGTGATTTGC TGTGGAATCC GATGGGGCCA 
GGCACGGAAG GCGTGTGGTT GCCGTGCAGC CTCAACGAAT GCTTCAAGAT CGGCCGCTAT 
CGCCCCGGGT GCCACTTCAG CGCGCACATG GACGGGCCGT GGGTGCCGAG CGCACACGAG 
GCCAGCATCT TCACCGTGCT CATCTACCTC AACGACGCCA CCACGTCGCC CGCCTGCCAC 
GGCGGACAGA CTAACTTCCT CGCAGCTGCC GCCGCCCACG CACCTGATGG CGCCAGCCCG 
CATGCGGCGC CACGTGACGA CGACCGGGTG GGGGCTCGGC TGGCGGAGGG GGTGAAGTAC 
GTGCTGCGCA CGGAGCTCAT CTTCAGGCGC GTCGACTCTC CGCTCACGCC GCGACACGTG 
TCGTCGGCTG ACGAGTCGGC GTGGCGCCGC ATCGTCGCCC TCTACGACCA ATCCGAGGAG 
CTGTTGCGAG AGGGCGACAC GGAGGGCTTT GTCGAGGCCT ATCTCGCCGC CATCGAGCTC 
CAGCAGCAGC GGACCAGCGA CAATGTCCAT CACCGCGTCG GCAATGGTGT CGACGGCGAT 
GACGTCGCCG CGCGCGGCTT GTTCACGGCG CTGCCGGCCG AGGTCGTGTG GCGCGTGACG 
AGCCGCCTGG GCGCGGGCGA TGTCGCGCGG CTGATGTGCG CCGCCGACAA GCGGCTCTAC 
CGCCTCTTGC GCGTCGATTC CTCGTGGCGC GCCTTCTATT TCCGTCGACT CGGGCTCAAC 
TACAGCGAGC GCATCCACCG CCGCGCTCTG CATGACGACT CTGACACCGC AGAGGAGCTG 
GAGGACGAGG CGATGCAGCT GCGCTTCGAC TGGTACGACC TGTGCCGCCG CCACAGCCGC 
AGACGCGGCG GACCCACCAC CTCAGGCGCG CCACGTGTCG CCACGTCAGC AGCGCCGATG 
ATGATGATGA AGAAGTGCGT CCAATCGGTC GACGACAGCG TGAAGCGGTC ACACGTGGCC 
GACGAGGCGC ACGATCTACA TCCCATGCGC CACGCAACTA TGCCGTCGTC GCCGATGTCG 
ACTAGTACGG TCCACCGTCG CGAGACCACC ACTACCACCG GCGGCGGGCT GCCGTTTCGG 
TGGATGCTGG GGGACGTCGG CGGGGACGTC GTGACGCCAC AGGCGGACTA CAGGCAGGAG 
GACGTCTCCT ATATCGTGTT GGACAATGGC GGCCACACCG TCAAAGCCGG GTTTGGAGGA 
GATGACGCGC CCCGGTCGCG CATTCCGGCG GTGGTGGGCT ACCCGCGACA CAGTCACTCG 
GTGCCGGTGG GCATGGGCAT CAAGTGGTTC TACGTGGGCG ACGAAGTCAT CGCCAAGCGG 
GGCATCCTCC GCCAAGAGAT CCCCATGCAC AATCGGACCA TCCTTAACTT CGACGCCATG 
GAACGCGTCT GGCACCATAC GTTCTACAAC GAGCTGCGGG TAGCGCCAGA GGAGCATCCG 
CTGGTGATGA GCCACACATC GTTCACGCCG CAGGCACACC TTGAAAAGAT GCTCCAGATC 
GCCATGGAGA CTTTCAACGT GCCCGCCTTC TTCTCCGCCA ACCAGCAAGT GTTGGCTAGC 
GTGTGGCTAG GGAAGAAGAC GGCCCTCGTG GTCGACATCG GTGTTCATGA CACCCACATC 
GTGCCCGTCG TTCAAGGAAG GGTGCTGGAG CAGGGCGTGG TGCGGCTGCC TTGCCTCGGC 
GGCGGGATGC TCACAGACCA CCTCCTCCAC CTCCTGACGC AGCAGCCCGG CAGCTCATTC 
GAGGAGAGCG GAGTCTACAG GGGCGTCGCC AATGAAATGA AGCACGCGTT GGGATTCTGT 
TCGCTGGCGC CAGAGCGTGA CCGCGAGGTT GCCGCGCGAC AGCTGCGCGA CGGCGCGCGC 
GAGCCGGTGC CGTTCTTCGA GTGTACGCGC GTGCGGGGCA CCTATCTCGA CGTGCTGCCC 
AGCGAGCTGC TGGTGCTGAT CGACAGGTCG GTGCAGCACC ACCGCATCGT TCGCCCCTAC 
TCCATGCCCG ACGAGAGCAA AGTGGTGGTG GGGAGTGAGC GGTTTGCCTG CGTGGAACCG 
CTCTTCCAAC CGCAGCTGCT CGACTGGGTG GTGGCCACCA AGCACAAGTT TATGTACCGG 
TGGTGTGACG AGGTGAACGA GGCGAGGAAG GGCATGCACG AGGGCCTGGC CCATAGCATC 
GACTCCTTCA AAGACGCCCC CGACCTCCAG TTAACCCACT CCCGCCTCCT TGTAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Genome biology14(2)R11(2013 Feb)
Clarke M,Lohan AJ,Liu B,Lagkouvardos I,Roy S,Zafar N,Bertelli C,Schilde C,Kianianmomeni A,B?rglin TR,Frech C,Turcotte B,Kopec KO,Synnott JM,Choo C,Paponov I,Finkler A,Heng Tan CS,Hutchins AP,Weinmeier T,Rattei T,Chu JS,Gimenez G,Irimia M,Rigden DJ,Fitzpatrick DA,Lorenzo-Morales J,Bateman A,Chiu CH,Tang P,Hegemann P,Fromm H,Raoult D,Greub G,Miranda-Saavedra D,Chen N,Nash P,Ginger ML,Horn M,Schaap P,Caler L,Loftus BJ

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ACA1_142240 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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