ACA1_112570 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene
Clones
gRNAs

Summary

Gene Symbol	ACA1_112570
Entrez Gene ID	14920801
Full Name	myosin light chain kinase
Gene Type	protein-coding
Organism	Acanthamoeba castellanii str. Neff

mRNA and Protein(s)

mRNA	Protein	Name
XM_004342023.1	XP_004342072.1	myosin light chain kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Clarke M, Lohan AJ, Liu B, Lagkouvardos I, Roy S, Zafar N, Bertelli C, Schilde C, Kianianmomeni A, B?rglin TR, Frech C, Turcotte B, Kopec KO, Synnott JM, Choo C, Paponov I, Finkler A, Heng Tan CS, Hutchins AP, Weinmeier T, Rattei T, Chu JS, Gimenez G, Irimia M, Rigden DJ, Fitzpatrick DA, Lorenzo-Morales J, Bateman A, Chiu CH, Tang P, Hegemann P, Fromm H, Raoult D, Greub G, Miranda-Saavedra D, Chen N, Nash P, Ginger ML, Horn M, Schaap P, Caler L, Loftus BJ
Genome biology14(2)R11(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACA1_112570 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACA1_112570 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc109462	XM_004342023.1 Latest version!	Acanthamoeba castellanii str. Neff myosin light chain kinase (ACA1_112570) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACA1_112570 ( XM_004342023.1 ) cDNA ORF clone, Acanthamoeba castellanii str. Neff -> XP_004342072.1 Acanthamoeba castellanii str. Neff myosin light chain kinase (ACA1_112570) mRNA, complete cds.

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CloneID	OAc109462
Clone ID Related Accession (Same CDS sequence)	XM_004342023.1
Accession Version	XM_004342023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-03-26
Organism	Acanthamoeba castellanii str. Neff
Product	myosin light chain kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_004457554). Long-term curation of the sequence and subsequent annotation updates will be the responsibility of the EuPathDB Bioinformatics Resource Center http://EuPathDB.org. Annotation can be viewed at http://AmoebaDB.org and inquires can be sent to [email protected]. COMPLETENESS: incomplete on both ends.

ATGGAGTGGT TGGGGCGCAC GATTTGGCCC AACAAAGGCT GCAACGAGGG GAGCGGCCTA 
CCGCCCGGCT TCAACAACGC CAAGCTGCAA CACAACAAGA TGGACCACCT CTACGAGCTG 
CGCGACCTCA TCCAAAACAA CAGCCACCAC ATACGCGCCG TCGTCGTAGA CCGCCGGTCC 
GCTACCTCTT CCTCCTCTGC ATCGCCTTCC TCTACACCAG CCCACGGGCC GCGGCTCTAC 
TCGATGCTGC GGGTGCTCAA GGCCGGGCAG TGCGAGGAGG CCTGGCTCCA GTGCCTGCGC 
GAGGCCGTCC AGGTCCACCA GCGCATGGCC GACCACCCAC ACATCGCGCG CCTCCGCCAC 
GTCTACGAAG ACGACGAAGA GCTCTGCGTC ATGTCCGATC ACTTGCGAGG AACGAGGCTC 
TATGATGAAG TGCTCCGTAG AGGCCGGTTC AAGGAAGCCG AAGTTATACC TCTGGTGAGC 
CATGGGGTGG GTCTGGCCAT CCGCCCCGAC GTCGTGTTCA TGGAGAACGC GAGCGTCGAC 
GGAGACGCAG CCTCGCCGCG CTACTCCCTC TCGCTTCCCG ATCTCTTCCT CATCATCGCG 
CAGAACATGG CCAAGGAGCG AAACGTGGAC ACCAACGGCT TCAGCGACAT GTCGGCGCCG 
CTGTGCTACA CCGCGCCCGA AGTGCTGCAA ATGGAGCGGT GCGGCGCCAC CTACTCCACC 
TTGTCCGCAG GCAACGCAGC AAGCGACGCG GCCGCTTCGC AGGGCGAGTC TGCTGCGTCA 
TCAGCGGCGG TCGCCGCTGC CGGCGTCATG GACGGCTCGC CCGGGGCGCG TTCCATGTGG 
GACACGTCGA AGAGCGACGT GTGGCAAATC GGCACGATCG CGTTCATGCT GCTCTCCGGC 
GGTGTGCCGT CGCATCCCTA CTTTGCCGCC GCTGGCAGCA GCGCCGCCGC CGAGTTTGCG 
CTCCGTCGCG ACGACATGAT GATCATCGCG AGTACGGGCG CGGAGGCGAT GACCATCAGC 
GAGCCGATGA TGTCACCAGG TCGCGCCTGC ACCTCGCCGC GGCTCGATTT CACCGGTGAC 
TGTTGGCTCG ATGTGTCCGA ATCCGCCAAG TGCTTCCTGG TGAGCCTGCT GGCCAACGAC 
CCGGCAAAGC GGCCAACGGC CCGGCAGGCC CTGCGCCACC CTTGGCTGTG CGGTGGCGCG 
ATGATGATGT TGGAGGACAT ATGCGTCGAT ACGATCGTGG ATCACGTGGA CGTGTTGCCC 
GACTACGCCG AGCGACTGCC CAAGTTTCTC GTGAAGAAGA TCACCACCAA CACCATCAAC 
TACCGCGAAA TCGAAGCCGT GGCCGTGGCC AGTGACACGT CATCAACGGC GCTGGTGGAC 
TCCAGCGCCG GCGCCGGCGA TCTCGGCCTG GGCGACCTGG CCAGTCGGCA CCTCTACGAG 
CTGGCCTCAG ACAACTTTCT GTGGAAGCGC CTGTGCCTCG CCACCATCAA GCGGGACCAG 
GGCCACGCCG ACCACCACCA CAACCGCGAC CGCGACCGCG ACGGCCACGA TGGCGACGGA 
GACGGCGATG ACCGAACGGC AGCGCCCCAG CACTTCGCTG AAGATCAGGT CAAGCAGATT 
ACGTACGAGA GCATCGAGGC CCAGTCGGCG TCGTCGCCGC TGTCGTCGCC GCCGCCGCTG 
TTCCTGTCGG CCTTCAAGCG GCGGCGGAAG AACACGAACA GCAGCGGCGG CGGCGGCGGC 
GGCAACAGTG CCGGCGGAGG GGGGAAGAAA AAGGCCCACG GCAAGGGGCG GAATTCGGAC 
TATGCCAGCG CTCTACTCGC CAGCATGGAC AAGAAACATC AAACGATAGA GGCCGTGAAC 
GGGCGCATCA ACACGGCGAA GAAAGTCGCA CCCGAGCTCT GGTGTACGGT GCTCGGAGGT 
CAGCTGATGC GGAAGGGCAC GCACACGTGG ACCGTTGAAA TCACGCACGC GGCCAACAGC 
CCGGGCGACA TCATGGTCGG CGTGTGCATG GCGGGCATGG ACGTGTCTAC AAGCCCGAGC 
TGGTTGTCTG ACAACGCCTG GCTGTACCAC ATCAACCAGC GCAGTATTTA CCACAAGGGC 
GTGTCCACGC TGGCAAGAAT CCAATGCGTT CTGCAAGGAA TCGACGTGCG ATTGATCGCC 
TGCACTTTCA ACATTGACGA AGGCACCCTC GCCATTTCAA TCAACGATCA CGACCAGGGC 
GTGGTCGCAC ACGTGCCGAC GGTGCTCGCG GACGGCCGCA GCGTCGCGTC AGTTGGGTTC 
TACGCAGCGG TGGACCTCTA CAACGCCGGC GACGCCGTCA CCCTCCGCTC CTTCTCCTCG 
ACGCCAACCA CCGCCACCAC CACCTCACCC CTCACCGCAG CAAGCCAGGC CGCCGCCGCC 
GGCAGCACCA CCACCACCAG CGGCAAGCCC GGCAAGGACC CGCTCTCCCC AGAGGTGACG 
CGGACAAAGA CGGAGAAGCG ACGGCTGGAC ATGTCGGTGT CGGGCAGCCT GATCGGCGAC 
AACGGCCTCC AGACCGTGCG CGTGACCAAA AAGTTCTGGA ATTCGAGGAA GACCGTCAAC 
TTCACGTGCT CCCTCACCCA GGCCCTCTTC CACCCGGGCA GCCAGATCGA CCTCGCCGTG 
AGTATCGACA ATCGGTCATC AAAGACGATT CGGTGTCTGA TCATTTCGTT CCGGGGCGTG 
AAGGGCAAGA AGGCGGTCCC GCTGGGCTAC TACTCCCACA CCAGCTTCTC GTCGACCGAG 
GGCACCATCT CCTACGAGCT CCACCTGGAG CTGCCCATCC GCGGGCACTC CTCGCTCAAG 
GCCGTGCTCC CGCTCCTTCA CATCCAGGGC TATCGCACCT CCGCTTCGCC CACCACCAGC 
ACCACGACCA CCAGCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004342072.1
CDS	1..2907
Translation	MEWLGRTIWPNKGCNEGSGLPPGFNNAKLQHNKMDHLYELRDLIQNNSHHIRAVVVDRRSATSSSSASPSSTPAHGPRLYSMLRVLKAGQCEEAWLQCLREAVQVHQRMADHPHIARLRHVYEDDEELCVMSDHLRGTRLYDEVLRRGRFKEAEVIPLVSHGVGLAIRPDVVFMENASVDGDAASPRYSLSLPDLFLIIAQNMAKERNVDTNGFSDMSAPLCYTAPEVLQMERCGATYSTLSAGNAASDAAASQGESAASSAAVAAAGVMDGSPGARSMWDTSKSDVWQIGTIAFMLLSGGVPSHPYFAAAGSSAAAEFALRRDDMMIIASTGAEAMTISEPMMSPGRACTSPRLDFTGDCWLDVSESAKCFLVSLLANDPAKRPTARQALRHPWLCGGAMMMLEDICVDTIVDHVDVLPDYAERLPKFLVKKITTNTINYREIEAVAVASDTSSTALVDSSAGAGDLGLGDLASRHLYELASDNFLWKRLCLATIKRDQGHADHHHNRDRDRDGHDGDGDGDDRTAAPQHFAEDQVKQITYESIEAQSASSPLSSPPPLFLSAFKRRRKNTNSSGGGGGGNSAGGGGKKKAHGKGRNSDYASALLASMDKKHQTIEAVNGRINTAKKVAPELWCTVLGGQLMRKGTHTWTVEITHAANSPGDIMVGVCMAGMDVSTSPSWLSDNAWLYHINQRSIYHKGVSTLARIQCVLQGIDVRLIACTFNIDEGTLAISINDHDQGVVAHVPTVLADGRSVASVGFYAAVDLYNAGDAVTLRSFSSTPTTATTTSPLTAASQAAAAGSTTTTSGKPGKDPLSPEVTRTKTEKRRLDMSVSGSLIGDNGLQTVRVTKKFWNSRKTVNFTCSLTQALFHPGSQIDLAVSIDNRSSKTIRCLIISFRGVKGKKAVPLGYYSHTSFSSTEGTISYELHLELPIRGHSSLKAVLPLLHIQGYRTSASPTTSTTTTSNEA

Target ORF information:

RefSeq Version	XM_004342023.1
Organism	Acanthamoeba castellanii str. Neff
Definition	Acanthamoeba castellanii str. Neff myosin light chain kinase (ACA1_112570) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004342023.1

ORF Insert Sequence:

ATGGAGTGGT TGGGGCGCAC GATTTGGCCC AACAAAGGCT GCAACGAGGG GAGCGGCCTA 
CCGCCCGGCT TCAACAACGC CAAGCTGCAA CACAACAAGA TGGACCACCT CTACGAGCTG 
CGCGACCTCA TCCAAAACAA CAGCCACCAC ATACGCGCCG TCGTCGTAGA CCGCCGGTCC 
GCTACCTCTT CCTCCTCTGC ATCGCCTTCC TCTACACCAG CCCACGGGCC GCGGCTCTAC 
TCGATGCTGC GGGTGCTCAA GGCCGGGCAG TGCGAGGAGG CCTGGCTCCA GTGCCTGCGC 
GAGGCCGTCC AGGTCCACCA GCGCATGGCC GACCACCCAC ACATCGCGCG CCTCCGCCAC 
GTCTACGAAG ACGACGAAGA GCTCTGCGTC ATGTCCGATC ACTTGCGAGG AACGAGGCTC 
TATGATGAAG TGCTCCGTAG AGGCCGGTTC AAGGAAGCCG AAGTTATACC TCTGGTGAGC 
CATGGGGTGG GTCTGGCCAT CCGCCCCGAC GTCGTGTTCA TGGAGAACGC GAGCGTCGAC 
GGAGACGCAG CCTCGCCGCG CTACTCCCTC TCGCTTCCCG ATCTCTTCCT CATCATCGCG 
CAGAACATGG CCAAGGAGCG AAACGTGGAC ACCAACGGCT TCAGCGACAT GTCGGCGCCG 
CTGTGCTACA CCGCGCCCGA AGTGCTGCAA ATGGAGCGGT GCGGCGCCAC CTACTCCACC 
TTGTCCGCAG GCAACGCAGC AAGCGACGCG GCCGCTTCGC AGGGCGAGTC TGCTGCGTCA 
TCAGCGGCGG TCGCCGCTGC CGGCGTCATG GACGGCTCGC CCGGGGCGCG TTCCATGTGG 
GACACGTCGA AGAGCGACGT GTGGCAAATC GGCACGATCG CGTTCATGCT GCTCTCCGGC 
GGTGTGCCGT CGCATCCCTA CTTTGCCGCC GCTGGCAGCA GCGCCGCCGC CGAGTTTGCG 
CTCCGTCGCG ACGACATGAT GATCATCGCG AGTACGGGCG CGGAGGCGAT GACCATCAGC 
GAGCCGATGA TGTCACCAGG TCGCGCCTGC ACCTCGCCGC GGCTCGATTT CACCGGTGAC 
TGTTGGCTCG ATGTGTCCGA ATCCGCCAAG TGCTTCCTGG TGAGCCTGCT GGCCAACGAC 
CCGGCAAAGC GGCCAACGGC CCGGCAGGCC CTGCGCCACC CTTGGCTGTG CGGTGGCGCG 
ATGATGATGT TGGAGGACAT ATGCGTCGAT ACGATCGTGG ATCACGTGGA CGTGTTGCCC 
GACTACGCCG AGCGACTGCC CAAGTTTCTC GTGAAGAAGA TCACCACCAA CACCATCAAC 
TACCGCGAAA TCGAAGCCGT GGCCGTGGCC AGTGACACGT CATCAACGGC GCTGGTGGAC 
TCCAGCGCCG GCGCCGGCGA TCTCGGCCTG GGCGACCTGG CCAGTCGGCA CCTCTACGAG 
CTGGCCTCAG ACAACTTTCT GTGGAAGCGC CTGTGCCTCG CCACCATCAA GCGGGACCAG 
GGCCACGCCG ACCACCACCA CAACCGCGAC CGCGACCGCG ACGGCCACGA TGGCGACGGA 
GACGGCGATG ACCGAACGGC AGCGCCCCAG CACTTCGCTG AAGATCAGGT CAAGCAGATT 
ACGTACGAGA GCATCGAGGC CCAGTCGGCG TCGTCGCCGC TGTCGTCGCC GCCGCCGCTG 
TTCCTGTCGG CCTTCAAGCG GCGGCGGAAG AACACGAACA GCAGCGGCGG CGGCGGCGGC 
GGCAACAGTG CCGGCGGAGG GGGGAAGAAA AAGGCCCACG GCAAGGGGCG GAATTCGGAC 
TATGCCAGCG CTCTACTCGC CAGCATGGAC AAGAAACATC AAACGATAGA GGCCGTGAAC 
GGGCGCATCA ACACGGCGAA GAAAGTCGCA CCCGAGCTCT GGTGTACGGT GCTCGGAGGT 
CAGCTGATGC GGAAGGGCAC GCACACGTGG ACCGTTGAAA TCACGCACGC GGCCAACAGC 
CCGGGCGACA TCATGGTCGG CGTGTGCATG GCGGGCATGG ACGTGTCTAC AAGCCCGAGC 
TGGTTGTCTG ACAACGCCTG GCTGTACCAC ATCAACCAGC GCAGTATTTA CCACAAGGGC 
GTGTCCACGC TGGCAAGAAT CCAATGCGTT CTGCAAGGAA TCGACGTGCG ATTGATCGCC 
TGCACTTTCA ACATTGACGA AGGCACCCTC GCCATTTCAA TCAACGATCA CGACCAGGGC 
GTGGTCGCAC ACGTGCCGAC GGTGCTCGCG GACGGCCGCA GCGTCGCGTC AGTTGGGTTC 
TACGCAGCGG TGGACCTCTA CAACGCCGGC GACGCCGTCA CCCTCCGCTC CTTCTCCTCG 
ACGCCAACCA CCGCCACCAC CACCTCACCC CTCACCGCAG CAAGCCAGGC CGCCGCCGCC 
GGCAGCACCA CCACCACCAG CGGCAAGCCC GGCAAGGACC CGCTCTCCCC AGAGGTGACG 
CGGACAAAGA CGGAGAAGCG ACGGCTGGAC ATGTCGGTGT CGGGCAGCCT GATCGGCGAC 
AACGGCCTCC AGACCGTGCG CGTGACCAAA AAGTTCTGGA ATTCGAGGAA GACCGTCAAC 
TTCACGTGCT CCCTCACCCA GGCCCTCTTC CACCCGGGCA GCCAGATCGA CCTCGCCGTG 
AGTATCGACA ATCGGTCATC AAAGACGATT CGGTGTCTGA TCATTTCGTT CCGGGGCGTG 
AAGGGCAAGA AGGCGGTCCC GCTGGGCTAC TACTCCCACA CCAGCTTCTC GTCGACCGAG 
GGCACCATCT CCTACGAGCT CCACCTGGAG CTGCCCATCC GCGGGCACTC CTCGCTCAAG 
GCCGTGCTCC CGCTCCTTCA CATCCAGGGC TATCGCACCT CCGCTTCGCC CACCACCAGC 
ACCACGACCA CCAGCAACGA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Genome biology14(2)R11(2013 Feb)
Clarke M,Lohan AJ,Liu B,Lagkouvardos I,Roy S,Zafar N,Bertelli C,Schilde C,Kianianmomeni A,B?rglin TR,Frech C,Turcotte B,Kopec KO,Synnott JM,Choo C,Paponov I,Finkler A,Heng Tan CS,Hutchins AP,Weinmeier T,Rattei T,Chu JS,Gimenez G,Irimia M,Rigden DJ,Fitzpatrick DA,Lorenzo-Morales J,Bateman A,Chiu CH,Tang P,Hegemann P,Fromm H,Raoult D,Greub G,Miranda-Saavedra D,Chen N,Nash P,Ginger ML,Horn M,Schaap P,Caler L,Loftus BJ

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ACA1_112570 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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