ACA1_198540 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene
Clones
gRNAs

Summary

Gene Symbol	ACA1_198540
Entrez Gene ID	14920457
Full Name	RecF/RecN/SMC domain containing protein
Gene Type	protein-coding
Organism	Acanthamoeba castellanii str. Neff

mRNA and Protein(s)

mRNA	Protein	Name
XM_004341677.1	XP_004341725.1	RecF/RecN/SMC domain containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Clarke M, Lohan AJ, Liu B, Lagkouvardos I, Roy S, Zafar N, Bertelli C, Schilde C, Kianianmomeni A, B?rglin TR, Frech C, Turcotte B, Kopec KO, Synnott JM, Choo C, Paponov I, Finkler A, Heng Tan CS, Hutchins AP, Weinmeier T, Rattei T, Chu JS, Gimenez G, Irimia M, Rigden DJ, Fitzpatrick DA, Lorenzo-Morales J, Bateman A, Chiu CH, Tang P, Hegemann P, Fromm H, Raoult D, Greub G, Miranda-Saavedra D, Chen N, Nash P, Ginger ML, Horn M, Schaap P, Caler L, Loftus BJ
Genome biology14(2)R11(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACA1_198540 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACA1_198540 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc109152	XM_004341677.1 Latest version!	Acanthamoeba castellanii str. Neff RecF/RecN/SMC domain containing protein (ACA1_198540) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACA1_198540 ( XM_004341677.1 ) cDNA ORF clone, Acanthamoeba castellanii str. Neff -> XP_004341725.1 Acanthamoeba castellanii str. Neff RecF/RecN/SMC domain containing protein (ACA1_198540) mRNA, complete cds.

Price & Availability↑

CloneID	OAc109152
Clone ID Related Accession (Same CDS sequence)	XM_004341677.1
Accession Version	XM_004341677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-03-26
Organism	Acanthamoeba castellanii str. Neff
Product	RecF/RecN/SMC domain containing protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_004457538). Long-term curation of the sequence and subsequent annotation updates will be the responsibility of the EuPathDB Bioinformatics Resource Center http://EuPathDB.org. Annotation can be viewed at http://AmoebaDB.org and inquires can be sent to [email protected]. COMPLETENESS: incomplete on both ends.

ATGCAGCAAA CGGAAGCCGA GGTGGGCATT ATTGAAATGA TCAAGCTCGA GAACTTCATG 
TGCCATCGTC ACCTGGAGCT GAAGCTGGGA CCCAACATCA ACTTCATCAT TGGCCAAAAT 
GGAAGCGGTA AGAGCGCCGT GCTGGTCGCA CTCACTGTGT GCTTGGGAGC AAAGGCTGGC 
TTCACCAATC GTGGAAAGAA GATTACCAAC TTCATCCGTG GAGACGCCAG CTCGGCCTCT 
GTATCTGTGA CGCTGCGCAA TCGTGGCGCG GAGGCCTACA AGCCCGACCT CTACGGCAAG 
ACCATCACCA TCATTAGGAC CATCGCACGG TCCGGCTCGT CGGGCTACAA AGTCAAGAGT 
GACAGCGGCA AGACGATCGC GACAACGAGG CGTGAGGTGC TGATGGTGAT GGAGCAGTTC 
AACATACAGA TCGAGAACCC CTGTGTGATC CTCATGCAGG ACACCAGCAG GGCGTTCTTG 
AACGCGAGTA AGCCCGCCGA GAAGTACAAG TTCTTCCTGA GCGCCACCCA GCTCCAGCAG 
ATCAGTGACG ACTACCGCAC AGTCGACGTC AAGCTGAACG GTATGAAGCA AACGCTGGAG 
AATAAGCAAG ACGTACTGCC CGACCTGAAG AAGAGGGTCC AGGAGCTGGA GCATCAGTTC 
AGGGAAGTGG CCAAGCTGCG CGACCTCGAA AGCCAGGTCC GCAAAATGAA GGGCCACCTG 
ATTTGGGCGC AGCTTCAAGG CCGAGAAAAA GAGCTGGCCG AGTTGCGCGA GAAGGTCGAG 
GCCCGCCAGC GCGAGATCGA CAAGAACGAC CAGGTCCTGG ACAAGATCAA GAACGCGACG 
CAAAAGCTCA CGGAGAAGTA CGAGGCCCGG CAGAAGCTGG TGGACGAAAC CGGCGCGCAG 
ATCCGTGCGG CCACCGCGGA GGTCGAGACC TACCGCACCC AGATCGGCGA GATCAAGCGC 
GGCATGAGCC ACCACCAGAA CACGGTCAAG GACCTGCGGA AGAGGCGCGA CGAGCTGGCG 
CTGCGTATCA GGAAGCTGCA GGAATTCATC AGCGACGTGC AGGAGAAGGC CATGCGCGAC 
ACACAAGCGG AGAAGCTGGA GAGGCAGGAG AGGATCGCGG CGCTGCAGGC GGCGTTGCAG 
GCGCAGCACG AGAAGGCCGA GGCGATGGAC CAGGAGCTCG CCGAACACGA GCGCCAGCGC 
CAGAGCATCG ACGCCGAACT GAAGGACCTC ACCGCCAAGG AGCGCGAGGT CGTTCAGGAG 
GCACAAAAAC TCGAGGGCCA GATCCGCACG CTGGAGCTGC AGAAGGCGGA CCGCACGCGC 
TGCTTCGGCC GAAACATGCC CGCCCTGCTC GACGCCATCA ACAGGGAGCG TCGGTGGCAA 
AAGAAGCCCA TCGGCCCGCT GGGCCTTTGC CTGACGATCA AGGACGACCA ATGGGCGACG 
GCCATCGAAA CCGCGCTCGG TCGTACCATG GACGCCTTTG TCGTCGACAA CCACCACGAC 
GAGAAGATCC TCAAGGAGAT CGGACGGCGC GTGGGGCAGG TGCCGGACAT GTATGTGCAG 
CGGTTCCAAG ACCGGGTCTA CCACATACCG GACAATGCGC TGCCTCCGCC CCACCTCACC 
ACCGTCCTCT CTCAGCTCCA GGCCGACGAA GTCATGGTGT ACAACTGCCT GATCGATCAG 
CGGCAGATCG ATAACATCAT TCTCATCCCG GATCGACAGC AGGCCTCACA GATCATGTTC 
CGACAACGGC CGCCGAACGC GAGAGAGGGC TACTTGCCGG ACGGCTCGCG ACTGCTGGTG 
CGCGGCGGCG CTGAAGTGTT CAACGCCGGA AACACGCGCG CGCGGAGGCT CGGCCGCAAT 
TTCGAGGAGC AGATCCGGGA CGCCCACGTT GCGCTCGAGC ACAAGCAGAA GGAGGCCGCG 
CACTTCAGGC CGCGCGTGCA GGAGCTGCGA AGCGCTGCCG ATGCCCTCCG TAAGCAACGG 
GCGGCGCTCA AGCGGGACGA GGGTCGGCTC ACGGCCGACA TCCACGAGCT CCAATCGGTG 
CGAGAGGAGG CCCCGCCCGA CATCTCCGAA GCTCAGACCA GTCTCGAGGG TTTTCTGAAG 
ACGAAGGAGG AGCTCGAGGC CGAGCTGGCC AAGGCCATGG AGGTGGCCGG CCAGGTGCCG 
GAGGAGCTGG GGCCGTTGCA GGACAAGTTC GCCGAGAAGA AGGCCGAGAT CGAGAACCTG 
GCCGCCCGCA GCGAACAGAT CGCCGCCAAG CTCACGAGCG TGCACAAGAA CATCACCACC 
ATAGCGGCGA GGGAGAAGCA GATGGTGCTC AACAAGGAGA AGCTGATGCA GTCCAAGCTG 
GAGGAGGAGA CGTCGCTGGC GGAGCAGCGC GCCGCGCTGG ACGAGGAAAT GCGCCGGGCC 
GAGCAGGCCG TCCCGCGGCC CGAGGAGGAC CTGCCGCCGC CGGCCCACCT CCAGCGCGAG 
ATCCAGAAAA TGGAGGCCCG CATCCGCTCG CAGACGGACG GCCAACGGAG TCCGGCGGAG 
ATCACGCGCG AGTTCAAGGC GGCCAAGAAG GCGTACGACG AGGCCACGTT CAAGGTGCAG 
CTGTTGAGCA GCCTGCTCGA ACTCCTCGAA AACGACCTCA GGGATCGTCT GAACAAGTGG 
CTCCTGTTCC GTCGCTCCAT CGCCAACCGG ACGACCTTCC ACTTCCACGG ATTCCTGAGC 
CGCAAGGGCT ACGCCGGGTC GCTCTCGTTC GACCACCAGC ACCGCCACCT GGACATCGAA 
GTGCAGCTCG ACGCTGCTCA GGGCGGTCCG CAGGGCGCAC GCGACACGCG AACCCTCTCC 
GGCGGCGAAC GCTCCTTCTC CACCGTCGCT CTCCTGCTCT CCCTGTGGGA AGCGATGGAG 
TCGCCCTTCC GTGCGATGGA CGAGTTCGAC GTGTTCATGG ACGCAGTGAA CCGCCACCTG 
TCGCTCAAGC TGCTGATCGA GTCGGCCCGA CAGCAGAGGC ACCGCCAGTT CATCTTCATC 
ACGCCACACG ACCTCACCTC CGTCGCGTCG GGACCCGACG TGCGGGTGAA CCGCATGCGC 
GACCCCGAGC GAGCCGACAT CGTCGGCATC GGCGCTCAGC AGACCATCCT CGACCAGTAC 
ACCCAGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004341725.1
CDS	1..3132
Translation	MQQTEAEVGIIEMIKLENFMCHRHLELKLGPNINFIIGQNGSGKSAVLVALTVCLGAKAGFTNRGKKITNFIRGDASSASVSVTLRNRGAEAYKPDLYGKTITIIRTIARSGSSGYKVKSDSGKTIATTRREVLMVMEQFNIQIENPCVILMQDTSRAFLNASKPAEKYKFFLSATQLQQISDDYRTVDVKLNGMKQTLENKQDVLPDLKKRVQELEHQFREVAKLRDLESQVRKMKGHLIWAQLQGREKELAELREKVEARQREIDKNDQVLDKIKNATQKLTEKYEARQKLVDETGAQIRAATAEVETYRTQIGEIKRGMSHHQNTVKDLRKRRDELALRIRKLQEFISDVQEKAMRDTQAEKLERQERIAALQAALQAQHEKAEAMDQELAEHERQRQSIDAELKDLTAKEREVVQEAQKLEGQIRTLELQKADRTRCFGRNMPALLDAINRERRWQKKPIGPLGLCLTIKDDQWATAIETALGRTMDAFVVDNHHDEKILKEIGRRVGQVPDMYVQRFQDRVYHIPDNALPPPHLTTVLSQLQADEVMVYNCLIDQRQIDNIILIPDRQQASQIMFRQRPPNAREGYLPDGSRLLVRGGAEVFNAGNTRARRLGRNFEEQIRDAHVALEHKQKEAAHFRPRVQELRSAADALRKQRAALKRDEGRLTADIHELQSVREEAPPDISEAQTSLEGFLKTKEELEAELAKAMEVAGQVPEELGPLQDKFAEKKAEIENLAARSEQIAAKLTSVHKNITTIAAREKQMVLNKEKLMQSKLEEETSLAEQRAALDEEMRRAEQAVPRPEEDLPPPAHLQREIQKMEARIRSQTDGQRSPAEITREFKAAKKAYDEATFKVQLLSSLLELLENDLRDRLNKWLLFRRSIANRTTFHFHGFLSRKGYAGSLSFDHQHRHLDIEVQLDAAQGGPQGARDTRTLSGGERSFSTVALLLSLWEAMESPFRAMDEFDVFMDAVNRHLSLKLLIESARQQRHRQFIFITPHDLTSVASGPDVRVNRMRDPERADIVGIGAQQTILDQYTQS

Target ORF information:

RefSeq Version	XM_004341677.1
Organism	Acanthamoeba castellanii str. Neff
Definition	Acanthamoeba castellanii str. Neff RecF/RecN/SMC domain containing protein (ACA1_198540) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004341677.1

ORF Insert Sequence:

ATGCAGCAAA CGGAAGCCGA GGTGGGCATT ATTGAAATGA TCAAGCTCGA GAACTTCATG 
TGCCATCGTC ACCTGGAGCT GAAGCTGGGA CCCAACATCA ACTTCATCAT TGGCCAAAAT 
GGAAGCGGTA AGAGCGCCGT GCTGGTCGCA CTCACTGTGT GCTTGGGAGC AAAGGCTGGC 
TTCACCAATC GTGGAAAGAA GATTACCAAC TTCATCCGTG GAGACGCCAG CTCGGCCTCT 
GTATCTGTGA CGCTGCGCAA TCGTGGCGCG GAGGCCTACA AGCCCGACCT CTACGGCAAG 
ACCATCACCA TCATTAGGAC CATCGCACGG TCCGGCTCGT CGGGCTACAA AGTCAAGAGT 
GACAGCGGCA AGACGATCGC GACAACGAGG CGTGAGGTGC TGATGGTGAT GGAGCAGTTC 
AACATACAGA TCGAGAACCC CTGTGTGATC CTCATGCAGG ACACCAGCAG GGCGTTCTTG 
AACGCGAGTA AGCCCGCCGA GAAGTACAAG TTCTTCCTGA GCGCCACCCA GCTCCAGCAG 
ATCAGTGACG ACTACCGCAC AGTCGACGTC AAGCTGAACG GTATGAAGCA AACGCTGGAG 
AATAAGCAAG ACGTACTGCC CGACCTGAAG AAGAGGGTCC AGGAGCTGGA GCATCAGTTC 
AGGGAAGTGG CCAAGCTGCG CGACCTCGAA AGCCAGGTCC GCAAAATGAA GGGCCACCTG 
ATTTGGGCGC AGCTTCAAGG CCGAGAAAAA GAGCTGGCCG AGTTGCGCGA GAAGGTCGAG 
GCCCGCCAGC GCGAGATCGA CAAGAACGAC CAGGTCCTGG ACAAGATCAA GAACGCGACG 
CAAAAGCTCA CGGAGAAGTA CGAGGCCCGG CAGAAGCTGG TGGACGAAAC CGGCGCGCAG 
ATCCGTGCGG CCACCGCGGA GGTCGAGACC TACCGCACCC AGATCGGCGA GATCAAGCGC 
GGCATGAGCC ACCACCAGAA CACGGTCAAG GACCTGCGGA AGAGGCGCGA CGAGCTGGCG 
CTGCGTATCA GGAAGCTGCA GGAATTCATC AGCGACGTGC AGGAGAAGGC CATGCGCGAC 
ACACAAGCGG AGAAGCTGGA GAGGCAGGAG AGGATCGCGG CGCTGCAGGC GGCGTTGCAG 
GCGCAGCACG AGAAGGCCGA GGCGATGGAC CAGGAGCTCG CCGAACACGA GCGCCAGCGC 
CAGAGCATCG ACGCCGAACT GAAGGACCTC ACCGCCAAGG AGCGCGAGGT CGTTCAGGAG 
GCACAAAAAC TCGAGGGCCA GATCCGCACG CTGGAGCTGC AGAAGGCGGA CCGCACGCGC 
TGCTTCGGCC GAAACATGCC CGCCCTGCTC GACGCCATCA ACAGGGAGCG TCGGTGGCAA 
AAGAAGCCCA TCGGCCCGCT GGGCCTTTGC CTGACGATCA AGGACGACCA ATGGGCGACG 
GCCATCGAAA CCGCGCTCGG TCGTACCATG GACGCCTTTG TCGTCGACAA CCACCACGAC 
GAGAAGATCC TCAAGGAGAT CGGACGGCGC GTGGGGCAGG TGCCGGACAT GTATGTGCAG 
CGGTTCCAAG ACCGGGTCTA CCACATACCG GACAATGCGC TGCCTCCGCC CCACCTCACC 
ACCGTCCTCT CTCAGCTCCA GGCCGACGAA GTCATGGTGT ACAACTGCCT GATCGATCAG 
CGGCAGATCG ATAACATCAT TCTCATCCCG GATCGACAGC AGGCCTCACA GATCATGTTC 
CGACAACGGC CGCCGAACGC GAGAGAGGGC TACTTGCCGG ACGGCTCGCG ACTGCTGGTG 
CGCGGCGGCG CTGAAGTGTT CAACGCCGGA AACACGCGCG CGCGGAGGCT CGGCCGCAAT 
TTCGAGGAGC AGATCCGGGA CGCCCACGTT GCGCTCGAGC ACAAGCAGAA GGAGGCCGCG 
CACTTCAGGC CGCGCGTGCA GGAGCTGCGA AGCGCTGCCG ATGCCCTCCG TAAGCAACGG 
GCGGCGCTCA AGCGGGACGA GGGTCGGCTC ACGGCCGACA TCCACGAGCT CCAATCGGTG 
CGAGAGGAGG CCCCGCCCGA CATCTCCGAA GCTCAGACCA GTCTCGAGGG TTTTCTGAAG 
ACGAAGGAGG AGCTCGAGGC CGAGCTGGCC AAGGCCATGG AGGTGGCCGG CCAGGTGCCG 
GAGGAGCTGG GGCCGTTGCA GGACAAGTTC GCCGAGAAGA AGGCCGAGAT CGAGAACCTG 
GCCGCCCGCA GCGAACAGAT CGCCGCCAAG CTCACGAGCG TGCACAAGAA CATCACCACC 
ATAGCGGCGA GGGAGAAGCA GATGGTGCTC AACAAGGAGA AGCTGATGCA GTCCAAGCTG 
GAGGAGGAGA CGTCGCTGGC GGAGCAGCGC GCCGCGCTGG ACGAGGAAAT GCGCCGGGCC 
GAGCAGGCCG TCCCGCGGCC CGAGGAGGAC CTGCCGCCGC CGGCCCACCT CCAGCGCGAG 
ATCCAGAAAA TGGAGGCCCG CATCCGCTCG CAGACGGACG GCCAACGGAG TCCGGCGGAG 
ATCACGCGCG AGTTCAAGGC GGCCAAGAAG GCGTACGACG AGGCCACGTT CAAGGTGCAG 
CTGTTGAGCA GCCTGCTCGA ACTCCTCGAA AACGACCTCA GGGATCGTCT GAACAAGTGG 
CTCCTGTTCC GTCGCTCCAT CGCCAACCGG ACGACCTTCC ACTTCCACGG ATTCCTGAGC 
CGCAAGGGCT ACGCCGGGTC GCTCTCGTTC GACCACCAGC ACCGCCACCT GGACATCGAA 
GTGCAGCTCG ACGCTGCTCA GGGCGGTCCG CAGGGCGCAC GCGACACGCG AACCCTCTCC 
GGCGGCGAAC GCTCCTTCTC CACCGTCGCT CTCCTGCTCT CCCTGTGGGA AGCGATGGAG 
TCGCCCTTCC GTGCGATGGA CGAGTTCGAC GTGTTCATGG ACGCAGTGAA CCGCCACCTG 
TCGCTCAAGC TGCTGATCGA GTCGGCCCGA CAGCAGAGGC ACCGCCAGTT CATCTTCATC 
ACGCCACACG ACCTCACCTC CGTCGCGTCG GGACCCGACG TGCGGGTGAA CCGCATGCGC 
GACCCCGAGC GAGCCGACAT CGTCGGCATC GGCGCTCAGC AGACCATCCT CGACCAGTAC 
ACCCAGTCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome of Acanthamoeba castellanii highlights extensive lateral gene transfer and early evolution of tyrosine kinase signaling.
Genome biology14(2)R11(2013 Feb)
Clarke M,Lohan AJ,Liu B,Lagkouvardos I,Roy S,Zafar N,Bertelli C,Schilde C,Kianianmomeni A,B?rglin TR,Frech C,Turcotte B,Kopec KO,Synnott JM,Choo C,Paponov I,Finkler A,Heng Tan CS,Hutchins AP,Weinmeier T,Rattei T,Chu JS,Gimenez G,Irimia M,Rigden DJ,Fitzpatrick DA,Lorenzo-Morales J,Bateman A,Chiu CH,Tang P,Hegemann P,Fromm H,Raoult D,Greub G,Miranda-Saavedra D,Chen N,Nash P,Ginger ML,Horn M,Schaap P,Caler L,Loftus BJ

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ACA1_198540 cDNA ORF clone, Acanthamoeba castellanii str. Neff

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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