LOC103457585 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103457585
Entrez Gene ID	103457585
Full Name	potassium voltage-gated channel subfamily H member 5-like
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017302333.1	XP_017157822.1	potassium voltage-gated channel subfamily H member 5-like isoform X2
XM_008397857.2	XP_008396079.1	potassium voltage-gated channel subfamily H member 5-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC103457585 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103457585 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo188118	XM_008397857.2 Latest version!	Poecilia reticulata potassium voltage-gated channel subfamily H member 5-like (LOC103457585), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo188119	XM_017302333.1 Latest version!	Poecilia reticulata potassium voltage-gated channel subfamily H member 5-like (LOC103457585), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103457585 ( XM_008397857.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008396079.1 Poecilia reticulata potassium voltage-gated channel subfamily H member 5-like (LOC103457585), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo188118
Clone ID Related Accession (Same CDS sequence)	XM_008397857.2
Accession Version	XM_008397857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	potassium voltage-gated channel subfamily H member 5-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024351.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008397857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAG GCAAGAGGGG TCTGGTGGCT CCGCAGAACA CTTTCCTGGA AAACATCGTC 
AGGCGCTCGA GCGAAACCAG TTTCCTTTTG GGCAATGCGC AGATCCTAGA TTGGCCAGTT 
GTATACAGCA ACGATGGATT CTGCAAGCTG TCCGGTTACC ACAGAGCCGA GGTCATGCAC 
AAGAGCAGCA CATGCAACTT CATGTACGGT GACCTGACCG ACAAAAAGAC TTTAGACAAA 
ATCAGGCAAA CATTTGACAG CCACGAGTCC AATTTCCACG AGGTGCTTCT GTACACAAAA 
AACAGAAAAC CAATATGGCT TTACATGCAG ATCGCACCGA TCAGGAATGA AAATGACAAG 
GTGGTGCTTT TCCTCTGCAC CTTCAGAGAC ATTACACTTT TTAAGCAGCC AATAGAAGAT 
GATTCAGCCA GAGGTTGGAC CAAATTTGCC AGGCTCACAA GGGCACTCAC CAACAACCGC 
AACCTGGTGC AGCAGATAGC GCCTCTGAAC AAACCTGAGG TCAGCCACAA GCCTTCCCGA 
CTGGTTGAGG CTCTCCAGTT GGGCTCAGAC ATCCTCCCTC AGTACAAGCA AGAGGCGCCA 
AAGACCCCTC CACACATCAT CCTCCACTAC TGCACCTTTA AGACCACCTG GGACTGGGTC 
ATCCTCATCC TCACGTTCTA CACGGCCATC ATGGTTCCCT ACAACGTCTC CTTCAGGACC 
AAGCACAACA ACCTGGCCTG GCTGGTGGTG GACAGCGTGG TCGACGTCAT CTTCCTGATC 
GACATTGTGC TCAACTTCCA CACAACCTTC GTGGGTCCGG CTGGAGAAGT CATCTCAGAC 
GCAAAGCTAA TCCGGATGAA TTACCTGAAG ACGTGGTTCG TCATCGACCT GCTGTCCTGT 
CTGCCCTACG ACATCATCAA CGCCTTTGAA AATGTGGAGG AGGACGTCAC TCCTCATGCC 
TCCCCGGCAA GTCATCTCCG TGACTTCTCA AATTTCCACA GAAACACAAC TCGCGCAGAG 
GACTCTGTTC TTCCAGGTCT CAGCAGCCTT TTCAGCTCCC TGAAGGTGAT CCGCCTGCTG 
CGTTTGGGCC GAGTGGCCCG GAAGCTGGAT CACTACCTGG AGTACGGGGC GGCCGTCCTG 
GTCCTCCTGG TGTGTGTCTT TGGCCTGGTA GCCCACTGGC TCGCCTGCAT CTGGTACAGC 
ATCGGGGACT ACGAGGTCAT CGACGAGACC ACCAACAGCA TCAAGACGGA CAGCTGGCTC 
TACCAGCTGG CCATGAGCAT CGGAAAGCCT TACCGGTACA ACGCCAGCGG CACGGGTCGG 
TGGGAAGGCG GCCCCGGCAA AGACACCCTG TACGTCTCCT CTCTCTATTT CACCATGACG 
AGTCTCACCA CCATTGGATT TGGCAATATT GCGCCAACAA CAGATGGAGA GAAGATTTTC 
TCCGTGGCTA TGATGATGGT GGGCTCGCTG CTCTACGCCA CCATCTTTGG AAACGTGACC 
ACCATCTTCC AGCAGATGTA CACCAACACA AACCGATACC ATGAGATGCT CAACAACGTG 
CGCGACTTCC TGAAGCTTTA CCAGGTTCCC AAAGGTCTGA GCGAGCGCGT CATGGACTTC 
ATCGTCTCCA CCTGGGCCAT GTCTAAAGGC ATCGACACAG ACAAGGTCCT CTCCATCTGT 
CCTAAAGACA TGCGTGCCGA CATCTGTGTG CACCTCAACA GGCAGGTGTT CAACGACCAT 
CCGGCGTTTC GTCTGGCGAG CGACGGCTGC TTGCGCTCGC TCGCCGTGGA GTTTCAGACG 
ACGCACTGCG CGCCGGGAGA CCTCATCTTC CATATCGGAG AAAGCGTCGA CACGCTCTGC 
TTTGTTGTCT CCGGCTCCCT CGAAGTCATC CAGGACGACG AGGTCATCGC AATACTCGGT 
AAAGGGGACG TGTTCGGGGA CGTCTTCTGG AAGGAGACCA CGCCGGCCCG GGCCTGCGCC 
AACGTGCGAG CGCTGACCTA CTGCGACCTG CACGTCATCA GGAGAGAAGC TCTGATGAGG 
GTCCTGGACT TTTACACCGC ATTTGCCAAC TCCTTCTCCC GCAATCTCAT CCTGACCTGT 
AACCTACGGA AACGGGTGAT CTTCAGGAAG ATCGCTGACG TGAAAAGAGA GCAGGAGCGG 
CAGAGGAGCG AGGTGGCGCT CTCCATTCCT GAGGATCACC CCGTTCGCAA GTTGTTCCAG 
AGGTTCAGGC AGCAGAGGGA CTCTCAAGCG CCAGGAGAGA CGCACCCTTA CTCCGACCAA 
AACTGCATCC AGATGGAGCC GCAGCACCAC GGTCACACCG ACTCAAACTC CTGCAATCAG 
CAGCAGGAGC ACGGCACCAA CATGCGCTCA GAAGCAGGGA GTGGAGGCAG GAGAGGCAGC 
GCGACTTCTC CTCACCAAGA GTGCAATGAC TCTGGAAAGT CAGAGCAGTT ATGCACCCAA 
GTGACCATGG AATTGAGGCC TAGTCAGAGG AGTTTAAAAC CTGCACAGAG CGGTGGGACG 
GGTGCAGGCG GAGCCAGCAG CAGAGGGGGG CGAGGGTGGG CGAGATTCAA GAGTGCCGTA 
TCTGTTGAAC CGGCGCTTCC TGCTGTTGAG CAGGAAAGGG ACACGCAGAA AGCGGAGGAG 
TGTTCGAAAG CACCGCAGGC CTCAGAGGAG CCTGCAGCTT CTAAAAAGAG CCAGGAATCA 
GAAGGCGGCG GACAGACGGG GAGCACGGAG GGCGGCAACA CAGCAGGAGA CGCGGCAGAG 
GAAACTAGCG CCCTGCACAA AACCGACTCC TGCGACAGCG GCATCACAAA GAGTGACCTG 
CGCATCGACC GAGCTGGAGA CTCCCGGAGC TCCTTTGAGA GGAGCCCGAT GGAAAAGAGC 
CCCCTGGACA GGAGTCCCTT CGAGCACAGC CTGGGGGTTT ACACGGAGGT ATCCCTGAGG 
CACTCCTTCC TCCAGCCGGT GTCTGAACAA TCCCTGCTGC AGATGACGCT GCACGAGGCC 
AAGCTGGAGC TGAAAGGAGA CATTAGGAAA CTGAGCAGTC GGCTGTCTCT CCTCGAGTCG 
CAAGTGAGCG AAGTCCTCAG GTTGCTGTCG TCCAAACGGA GACTCTCGCT GCCTCCGACT 
TCTTCCAAGG CGAAAATAAA AAGTCACGAC GTGATGGCGT CCTCTAGGGT CGTGGCGCCA 
AAAGCAGAAG AGGAATCTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008396079.1
CDS	88..3291
Translation	MHGGKRGLVAPQNTFLENIVRRSSETSFLLGNAQILDWPVVYSNDGFCKLSGYHRAEVMHKSSTCNFMYGDLTDKKTLDKIRQTFDSHESNFHEVLLYTKNRKPIWLYMQIAPIRNENDKVVLFLCTFRDITLFKQPIEDDSARGWTKFARLTRALTNNRNLVQQIAPLNKPEVSHKPSRLVEALQLGSDILPQYKQEAPKTPPHIILHYCTFKTTWDWVILILTFYTAIMVPYNVSFRTKHNNLAWLVVDSVVDVIFLIDIVLNFHTTFVGPAGEVISDAKLIRMNYLKTWFVIDLLSCLPYDIINAFENVEEDVTPHASPASHLRDFSNFHRNTTRAEDSVLPGLSSLFSSLKVIRLLRLGRVARKLDHYLEYGAAVLVLLVCVFGLVAHWLACIWYSIGDYEVIDETTNSIKTDSWLYQLAMSIGKPYRYNASGTGRWEGGPGKDTLYVSSLYFTMTSLTTIGFGNIAPTTDGEKIFSVAMMMVGSLLYATIFGNVTTIFQQMYTNTNRYHEMLNNVRDFLKLYQVPKGLSERVMDFIVSTWAMSKGIDTDKVLSICPKDMRADICVHLNRQVFNDHPAFRLASDGCLRSLAVEFQTTHCAPGDLIFHIGESVDTLCFVVSGSLEVIQDDEVIAILGKGDVFGDVFWKETTPARACANVRALTYCDLHVIRREALMRVLDFYTAFANSFSRNLILTCNLRKRVIFRKIADVKREQERQRSEVALSIPEDHPVRKLFQRFRQQRDSQAPGETHPYSDQNCIQMEPQHHGHTDSNSCNQQQEHGTNMRSEAGSGGRRGSATSPHQECNDSGKSEQLCTQVTMELRPSQRSLKPAQSGGTGAGGASSRGGRGWARFKSAVSVEPALPAVEQERDTQKAEECSKAPQASEEPAASKKSQESEGGGQTGSTEGGNTAGDAAEETSALHKTDSCDSGITKSDLRIDRAGDSRSSFERSPMEKSPLDRSPFEHSLGVYTEVSLRHSFLQPVSEQSLLQMTLHEAKLELKGDIRKLSSRLSLLESQVSEVLRLLSSKRRLSLPPTSSKAKIKSHDVMASSRVVAPKAEEESL

Target ORF information:

RefSeq Version	XM_008397857.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata potassium voltage-gated channel subfamily H member 5-like (LOC103457585), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008397857.2

ORF Insert Sequence:

ATGCATGGAG GCAAGAGGGG TCTGGTGGCT CCGCAGAACA CTTTCCTGGA AAACATCGTC 
AGGCGCTCGA GCGAAACCAG TTTCCTTTTG GGCAATGCGC AGATCCTAGA TTGGCCAGTT 
GTATACAGCA ACGATGGATT CTGCAAGCTG TCCGGTTACC ACAGAGCCGA GGTCATGCAC 
AAGAGCAGCA CATGCAACTT CATGTACGGT GACCTGACCG ACAAAAAGAC TTTAGACAAA 
ATCAGGCAAA CATTTGACAG CCACGAGTCC AATTTCCACG AGGTGCTTCT GTACACAAAA 
AACAGAAAAC CAATATGGCT TTACATGCAG ATCGCACCGA TCAGGAATGA AAATGACAAG 
GTGGTGCTTT TCCTCTGCAC CTTCAGAGAC ATTACACTTT TTAAGCAGCC AATAGAAGAT 
GATTCAGCCA GAGGTTGGAC CAAATTTGCC AGGCTCACAA GGGCACTCAC CAACAACCGC 
AACCTGGTGC AGCAGATAGC GCCTCTGAAC AAACCTGAGG TCAGCCACAA GCCTTCCCGA 
CTGGTTGAGG CTCTCCAGTT GGGCTCAGAC ATCCTCCCTC AGTACAAGCA AGAGGCGCCA 
AAGACCCCTC CACACATCAT CCTCCACTAC TGCACCTTTA AGACCACCTG GGACTGGGTC 
ATCCTCATCC TCACGTTCTA CACGGCCATC ATGGTTCCCT ACAACGTCTC CTTCAGGACC 
AAGCACAACA ACCTGGCCTG GCTGGTGGTG GACAGCGTGG TCGACGTCAT CTTCCTGATC 
GACATTGTGC TCAACTTCCA CACAACCTTC GTGGGTCCGG CTGGAGAAGT CATCTCAGAC 
GCAAAGCTAA TCCGGATGAA TTACCTGAAG ACGTGGTTCG TCATCGACCT GCTGTCCTGT 
CTGCCCTACG ACATCATCAA CGCCTTTGAA AATGTGGAGG AGGACGTCAC TCCTCATGCC 
TCCCCGGCAA GTCATCTCCG TGACTTCTCA AATTTCCACA GAAACACAAC TCGCGCAGAG 
GACTCTGTTC TTCCAGGTCT CAGCAGCCTT TTCAGCTCCC TGAAGGTGAT CCGCCTGCTG 
CGTTTGGGCC GAGTGGCCCG GAAGCTGGAT CACTACCTGG AGTACGGGGC GGCCGTCCTG 
GTCCTCCTGG TGTGTGTCTT TGGCCTGGTA GCCCACTGGC TCGCCTGCAT CTGGTACAGC 
ATCGGGGACT ACGAGGTCAT CGACGAGACC ACCAACAGCA TCAAGACGGA CAGCTGGCTC 
TACCAGCTGG CCATGAGCAT CGGAAAGCCT TACCGGTACA ACGCCAGCGG CACGGGTCGG 
TGGGAAGGCG GCCCCGGCAA AGACACCCTG TACGTCTCCT CTCTCTATTT CACCATGACG 
AGTCTCACCA CCATTGGATT TGGCAATATT GCGCCAACAA CAGATGGAGA GAAGATTTTC 
TCCGTGGCTA TGATGATGGT GGGCTCGCTG CTCTACGCCA CCATCTTTGG AAACGTGACC 
ACCATCTTCC AGCAGATGTA CACCAACACA AACCGATACC ATGAGATGCT CAACAACGTG 
CGCGACTTCC TGAAGCTTTA CCAGGTTCCC AAAGGTCTGA GCGAGCGCGT CATGGACTTC 
ATCGTCTCCA CCTGGGCCAT GTCTAAAGGC ATCGACACAG ACAAGGTCCT CTCCATCTGT 
CCTAAAGACA TGCGTGCCGA CATCTGTGTG CACCTCAACA GGCAGGTGTT CAACGACCAT 
CCGGCGTTTC GTCTGGCGAG CGACGGCTGC TTGCGCTCGC TCGCCGTGGA GTTTCAGACG 
ACGCACTGCG CGCCGGGAGA CCTCATCTTC CATATCGGAG AAAGCGTCGA CACGCTCTGC 
TTTGTTGTCT CCGGCTCCCT CGAAGTCATC CAGGACGACG AGGTCATCGC AATACTCGGT 
AAAGGGGACG TGTTCGGGGA CGTCTTCTGG AAGGAGACCA CGCCGGCCCG GGCCTGCGCC 
AACGTGCGAG CGCTGACCTA CTGCGACCTG CACGTCATCA GGAGAGAAGC TCTGATGAGG 
GTCCTGGACT TTTACACCGC ATTTGCCAAC TCCTTCTCCC GCAATCTCAT CCTGACCTGT 
AACCTACGGA AACGGGTGAT CTTCAGGAAG ATCGCTGACG TGAAAAGAGA GCAGGAGCGG 
CAGAGGAGCG AGGTGGCGCT CTCCATTCCT GAGGATCACC CCGTTCGCAA GTTGTTCCAG 
AGGTTCAGGC AGCAGAGGGA CTCTCAAGCG CCAGGAGAGA CGCACCCTTA CTCCGACCAA 
AACTGCATCC AGATGGAGCC GCAGCACCAC GGTCACACCG ACTCAAACTC CTGCAATCAG 
CAGCAGGAGC ACGGCACCAA CATGCGCTCA GAAGCAGGGA GTGGAGGCAG GAGAGGCAGC 
GCGACTTCTC CTCACCAAGA GTGCAATGAC TCTGGAAAGT CAGAGCAGTT ATGCACCCAA 
GTGACCATGG AATTGAGGCC TAGTCAGAGG AGTTTAAAAC CTGCACAGAG CGGTGGGACG 
GGTGCAGGCG GAGCCAGCAG CAGAGGGGGG CGAGGGTGGG CGAGATTCAA GAGTGCCGTA 
TCTGTTGAAC CGGCGCTTCC TGCTGTTGAG CAGGAAAGGG ACACGCAGAA AGCGGAGGAG 
TGTTCGAAAG CACCGCAGGC CTCAGAGGAG CCTGCAGCTT CTAAAAAGAG CCAGGAATCA 
GAAGGCGGCG GACAGACGGG GAGCACGGAG GGCGGCAACA CAGCAGGAGA CGCGGCAGAG 
GAAACTAGCG CCCTGCACAA AACCGACTCC TGCGACAGCG GCATCACAAA GAGTGACCTG 
CGCATCGACC GAGCTGGAGA CTCCCGGAGC TCCTTTGAGA GGAGCCCGAT GGAAAAGAGC 
CCCCTGGACA GGAGTCCCTT CGAGCACAGC CTGGGGGTTT ACACGGAGGT ATCCCTGAGG 
CACTCCTTCC TCCAGCCGGT GTCTGAACAA TCCCTGCTGC AGATGACGCT GCACGAGGCC 
AAGCTGGAGC TGAAAGGAGA CATTAGGAAA CTGAGCAGTC GGCTGTCTCT CCTCGAGTCG 
CAAGTGAGCG AAGTCCTCAG GTTGCTGTCG TCCAAACGGA GACTCTCGCT GCCTCCGACT 
TCTTCCAAGG CGAAAATAAA AAGTCACGAC GTGATGGCGT CCTCTAGGGT CGTGGCGCCA 
AAAGCAGAAG AGGAATCTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103457585 ( XM_017302333.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017157822.1 Poecilia reticulata potassium voltage-gated channel subfamily H member 5-like (LOC103457585), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo188119
Clone ID Related Accession (Same CDS sequence)	XM_017302333.1
Accession Version	XM_017302333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	potassium voltage-gated channel subfamily H member 5-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024351.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGGAG GCAAGAGGGG TCTGGTGGCT CCGCAGAACA CTTTCCTGGA AAACATCGTC 
AGGCGCTCGA GCGAAACCAG TTTCCTTTTG GGCAATGCGC AGATCCTAGA TTGGCCAGTT 
GTATACAGCA ACGATGGATT CTGCAAGCTG TCCGGTTACC ACAGAGCCGA GGTCATGCAC 
AAGAGCAGCA CATGCAACTT CATGTACGGT GACCTGACCG ACAAAAAGAC TTTAGACAAA 
ATCAGGCAAA CATTTGACAG CCACGAGTCC AATTTCCACG AGGTGCTTCT GTACACAAAA 
AACAGAAAAC CAATATGGCT TTACATGCAG ATCGCACCGA TCAGGAATGA AAATGACAAG 
GTGGTGCTTT TCCTCTGCAC CTTCAGAGAC ATTACACTTT TTAAGCAGCC AATAGAAGAT 
GATTCAGCCA GAGGTTGGAC CAAATTTGCC AGGCTCACAA GGGCACTCAC CAACAACCGC 
AACCTGGTGC AGCAGATAGC GCCTCTGAAC AAACCTGAGG TCAGCCACAA GCCTTCCCGA 
CTGGTTGAGG CTCTCCAGTT GGGCTCAGAC ATCCTCCCTC AGTACAAGCA AGAGGCGCCA 
AAGACCCCTC CACACATCAT CCTCCACTAC TGCACCTTTA AGACCACCTG GGACTGGGTC 
ATCCTCATCC TCACGTTCTA CACGGCCATC ATGGTTCCCT ACAACGTCTC CTTCAGGACC 
AAGCACAACA ACCTGGCCTG GCTGGTGGTG GACAGCGTGG TCGACGTCAT CTTCCTGATC 
GACATTGTGC TCAACTTCCA CACAACCTTC GTGGGTCCGG CTGGAGAAGT CATCTCAGAC 
GCAAAGCTAA TCCGGATGAA TTACCTGAAG ACGTGGTTCG TCATCGACCT GCTGTCCTGT 
CTGCCCTACG ACATCATCAA CGCCTTTGAA AATGTGGAGG AGGTAAACGG CCTAACAGAT 
CAAACCTTTA GAATAACGTG TGCTTTTGTC CTGCAGGGTC TCAGCAGCCT TTTCAGCTCC 
CTGAAGGTGA TCCGCCTGCT GCGTTTGGGC CGAGTGGCCC GGAAGCTGGA TCACTACCTG 
GAGTACGGGG CGGCCGTCCT GGTCCTCCTG GTGTGTGTCT TTGGCCTGGT AGCCCACTGG 
CTCGCCTGCA TCTGGTACAG CATCGGGGAC TACGAGGTCA TCGACGAGAC CACCAACAGC 
ATCAAGACGG ACAGCTGGCT CTACCAGCTG GCCATGAGCA TCGGAAAGCC TTACCGGTAC 
AACGCCAGCG GCACGGGTCG GTGGGAAGGC GGCCCCGGCA AAGACACCCT GTACGTCTCC 
TCTCTCTATT TCACCATGAC GAGTCTCACC ACCATTGGAT TTGGCAATAT TGCGCCAACA 
ACAGATGGAG AGAAGATTTT CTCCGTGGCT ATGATGATGG TGGGCTCGCT GCTCTACGCC 
ACCATCTTTG GAAACGTGAC CACCATCTTC CAGCAGATGT ACACCAACAC AAACCGATAC 
CATGAGATGC TCAACAACGT GCGCGACTTC CTGAAGCTTT ACCAGGTTCC CAAAGGTCTG 
AGCGAGCGCG TCATGGACTT CATCGTCTCC ACCTGGGCCA TGTCTAAAGG CATCGACACA 
GACAAGGTCC TCTCCATCTG TCCTAAAGAC ATGCGTGCCG ACATCTGTGT GCACCTCAAC 
AGGCAGGTGT TCAACGACCA TCCGGCGTTT CGTCTGGCGA GCGACGGCTG CTTGCGCTCG 
CTCGCCGTGG AGTTTCAGAC GACGCACTGC GCGCCGGGAG ACCTCATCTT CCATATCGGA 
GAAAGCGTCG ACACGCTCTG CTTTGTTGTC TCCGGCTCCC TCGAAGTCAT CCAGGACGAC 
GAGGTCATCG CAATACTCGG TAAAGGGGAC GTGTTCGGGG ACGTCTTCTG GAAGGAGACC 
ACGCCGGCCC GGGCCTGCGC CAACGTGCGA GCGCTGACCT ACTGCGACCT GCACGTCATC 
AGGAGAGAAG CTCTGATGAG GGTCCTGGAC TTTTACACCG CATTTGCCAA CTCCTTCTCC 
CGCAATCTCA TCCTGACCTG TAACCTACGG AAACGGGTGA TCTTCAGGAA GATCGCTGAC 
GTGAAAAGAG AGCAGGAGCG GCAGAGGAGC GAGGTGGCGC TCTCCATTCC TGAGGATCAC 
CCCGTTCGCA AGTTGTTCCA GAGGTTCAGG CAGCAGAGGG ACTCTCAAGC GCCAGGAGAG 
ACGCACCCTT ACTCCGACCA AAACTGCATC CAGATGGAGC CGCAGCACCA CGGTCACACC 
GACTCAAACT CCTGCAATCA GCAGCAGGAG CACGGCACCA ACATGCGCTC AGAAGCAGGG 
AGTGGAGGCA GGAGAGGCAG CGCGACTTCT CCTCACCAAG AGTGCAATGA CTCTGGAAAG 
TCAGAGCAGT TATGCACCCA AGTGACCATG GAATTGAGGC CTAGTCAGAG GAGTTTAAAA 
CCTGCACAGA GCGGTGGGAC GGGTGCAGGC GGAGCCAGCA GCAGAGGGGG GCGAGGGTGG 
GCGAGATTCA AGAGTGCCGT ATCTGTTGAA CCGGCGCTTC CTGCTGTTGA GCAGGAAAGG 
GACACGCAGA AAGCGGAGGA GTGTTCGAAA GCACCGCAGG CCTCAGAGGA GCCTGCAGCT 
TCTAAAAAGA GCCAGGAATC AGAAGGCGGC GGACAGACGG GGAGCACGGA GGGCGGCAAC 
ACAGCAGGAG ACGCGGCAGA GGAAACTAGC GCCCTGCACA AAACCGACTC CTGCGACAGC 
GGCATCACAA AGAGTGACCT GCGCATCGAC CGAGCTGGAG ACTCCCGGAG CTCCTTTGAG 
AGGAGCCCGA TGGAAAAGAG CCCCCTGGAC AGGAGTCCCT TCGAGCACAG CCTGGGGGTT 
TACACGGAGG TATCCCTGAG GCACTCCTTC CTCCAGCCGG TGTCTGAACA ATCCCTGCTG 
CAGATGACGC TGCACGAGGC CAAGCTGGAG CTGAAAGGAG ACATTAGGAA ACTGAGCAGT 
CGGCTGTCTC TCCTCGAGTC GCAAGTGAGC GAAGTCCTCA GGTTGCTGTC GTCCAAACGG 
AGACTCTCGC TGCCTCCGAC TTCTTCCAAG GCGAAAATAA AAAGTCACGA CGTGATGGCG 
TCCTCTAGGG TCGTGGCGCC AAAAGCAGAA GAGGAATCTT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017157822.1
CDS	88..3252
Translation	MHGGKRGLVAPQNTFLENIVRRSSETSFLLGNAQILDWPVVYSNDGFCKLSGYHRAEVMHKSSTCNFMYGDLTDKKTLDKIRQTFDSHESNFHEVLLYTKNRKPIWLYMQIAPIRNENDKVVLFLCTFRDITLFKQPIEDDSARGWTKFARLTRALTNNRNLVQQIAPLNKPEVSHKPSRLVEALQLGSDILPQYKQEAPKTPPHIILHYCTFKTTWDWVILILTFYTAIMVPYNVSFRTKHNNLAWLVVDSVVDVIFLIDIVLNFHTTFVGPAGEVISDAKLIRMNYLKTWFVIDLLSCLPYDIINAFENVEEVNGLTDQTFRITCAFVLQGLSSLFSSLKVIRLLRLGRVARKLDHYLEYGAAVLVLLVCVFGLVAHWLACIWYSIGDYEVIDETTNSIKTDSWLYQLAMSIGKPYRYNASGTGRWEGGPGKDTLYVSSLYFTMTSLTTIGFGNIAPTTDGEKIFSVAMMMVGSLLYATIFGNVTTIFQQMYTNTNRYHEMLNNVRDFLKLYQVPKGLSERVMDFIVSTWAMSKGIDTDKVLSICPKDMRADICVHLNRQVFNDHPAFRLASDGCLRSLAVEFQTTHCAPGDLIFHIGESVDTLCFVVSGSLEVIQDDEVIAILGKGDVFGDVFWKETTPARACANVRALTYCDLHVIRREALMRVLDFYTAFANSFSRNLILTCNLRKRVIFRKIADVKREQERQRSEVALSIPEDHPVRKLFQRFRQQRDSQAPGETHPYSDQNCIQMEPQHHGHTDSNSCNQQQEHGTNMRSEAGSGGRRGSATSPHQECNDSGKSEQLCTQVTMELRPSQRSLKPAQSGGTGAGGASSRGGRGWARFKSAVSVEPALPAVEQERDTQKAEECSKAPQASEEPAASKKSQESEGGGQTGSTEGGNTAGDAAEETSALHKTDSCDSGITKSDLRIDRAGDSRSSFERSPMEKSPLDRSPFEHSLGVYTEVSLRHSFLQPVSEQSLLQMTLHEAKLELKGDIRKLSSRLSLLESQVSEVLRLLSSKRRLSLPPTSSKAKIKSHDVMASSRVVAPKAEEESL

Target ORF information:

RefSeq Version	XM_017302333.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata potassium voltage-gated channel subfamily H member 5-like (LOC103457585), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017302333.1

ORF Insert Sequence:

ATGCATGGAG GCAAGAGGGG TCTGGTGGCT CCGCAGAACA CTTTCCTGGA AAACATCGTC 
AGGCGCTCGA GCGAAACCAG TTTCCTTTTG GGCAATGCGC AGATCCTAGA TTGGCCAGTT 
GTATACAGCA ACGATGGATT CTGCAAGCTG TCCGGTTACC ACAGAGCCGA GGTCATGCAC 
AAGAGCAGCA CATGCAACTT CATGTACGGT GACCTGACCG ACAAAAAGAC TTTAGACAAA 
ATCAGGCAAA CATTTGACAG CCACGAGTCC AATTTCCACG AGGTGCTTCT GTACACAAAA 
AACAGAAAAC CAATATGGCT TTACATGCAG ATCGCACCGA TCAGGAATGA AAATGACAAG 
GTGGTGCTTT TCCTCTGCAC CTTCAGAGAC ATTACACTTT TTAAGCAGCC AATAGAAGAT 
GATTCAGCCA GAGGTTGGAC CAAATTTGCC AGGCTCACAA GGGCACTCAC CAACAACCGC 
AACCTGGTGC AGCAGATAGC GCCTCTGAAC AAACCTGAGG TCAGCCACAA GCCTTCCCGA 
CTGGTTGAGG CTCTCCAGTT GGGCTCAGAC ATCCTCCCTC AGTACAAGCA AGAGGCGCCA 
AAGACCCCTC CACACATCAT CCTCCACTAC TGCACCTTTA AGACCACCTG GGACTGGGTC 
ATCCTCATCC TCACGTTCTA CACGGCCATC ATGGTTCCCT ACAACGTCTC CTTCAGGACC 
AAGCACAACA ACCTGGCCTG GCTGGTGGTG GACAGCGTGG TCGACGTCAT CTTCCTGATC 
GACATTGTGC TCAACTTCCA CACAACCTTC GTGGGTCCGG CTGGAGAAGT CATCTCAGAC 
GCAAAGCTAA TCCGGATGAA TTACCTGAAG ACGTGGTTCG TCATCGACCT GCTGTCCTGT 
CTGCCCTACG ACATCATCAA CGCCTTTGAA AATGTGGAGG AGGTAAACGG CCTAACAGAT 
CAAACCTTTA GAATAACGTG TGCTTTTGTC CTGCAGGGTC TCAGCAGCCT TTTCAGCTCC 
CTGAAGGTGA TCCGCCTGCT GCGTTTGGGC CGAGTGGCCC GGAAGCTGGA TCACTACCTG 
GAGTACGGGG CGGCCGTCCT GGTCCTCCTG GTGTGTGTCT TTGGCCTGGT AGCCCACTGG 
CTCGCCTGCA TCTGGTACAG CATCGGGGAC TACGAGGTCA TCGACGAGAC CACCAACAGC 
ATCAAGACGG ACAGCTGGCT CTACCAGCTG GCCATGAGCA TCGGAAAGCC TTACCGGTAC 
AACGCCAGCG GCACGGGTCG GTGGGAAGGC GGCCCCGGCA AAGACACCCT GTACGTCTCC 
TCTCTCTATT TCACCATGAC GAGTCTCACC ACCATTGGAT TTGGCAATAT TGCGCCAACA 
ACAGATGGAG AGAAGATTTT CTCCGTGGCT ATGATGATGG TGGGCTCGCT GCTCTACGCC 
ACCATCTTTG GAAACGTGAC CACCATCTTC CAGCAGATGT ACACCAACAC AAACCGATAC 
CATGAGATGC TCAACAACGT GCGCGACTTC CTGAAGCTTT ACCAGGTTCC CAAAGGTCTG 
AGCGAGCGCG TCATGGACTT CATCGTCTCC ACCTGGGCCA TGTCTAAAGG CATCGACACA 
GACAAGGTCC TCTCCATCTG TCCTAAAGAC ATGCGTGCCG ACATCTGTGT GCACCTCAAC 
AGGCAGGTGT TCAACGACCA TCCGGCGTTT CGTCTGGCGA GCGACGGCTG CTTGCGCTCG 
CTCGCCGTGG AGTTTCAGAC GACGCACTGC GCGCCGGGAG ACCTCATCTT CCATATCGGA 
GAAAGCGTCG ACACGCTCTG CTTTGTTGTC TCCGGCTCCC TCGAAGTCAT CCAGGACGAC 
GAGGTCATCG CAATACTCGG TAAAGGGGAC GTGTTCGGGG ACGTCTTCTG GAAGGAGACC 
ACGCCGGCCC GGGCCTGCGC CAACGTGCGA GCGCTGACCT ACTGCGACCT GCACGTCATC 
AGGAGAGAAG CTCTGATGAG GGTCCTGGAC TTTTACACCG CATTTGCCAA CTCCTTCTCC 
CGCAATCTCA TCCTGACCTG TAACCTACGG AAACGGGTGA TCTTCAGGAA GATCGCTGAC 
GTGAAAAGAG AGCAGGAGCG GCAGAGGAGC GAGGTGGCGC TCTCCATTCC TGAGGATCAC 
CCCGTTCGCA AGTTGTTCCA GAGGTTCAGG CAGCAGAGGG ACTCTCAAGC GCCAGGAGAG 
ACGCACCCTT ACTCCGACCA AAACTGCATC CAGATGGAGC CGCAGCACCA CGGTCACACC 
GACTCAAACT CCTGCAATCA GCAGCAGGAG CACGGCACCA ACATGCGCTC AGAAGCAGGG 
AGTGGAGGCA GGAGAGGCAG CGCGACTTCT CCTCACCAAG AGTGCAATGA CTCTGGAAAG 
TCAGAGCAGT TATGCACCCA AGTGACCATG GAATTGAGGC CTAGTCAGAG GAGTTTAAAA 
CCTGCACAGA GCGGTGGGAC GGGTGCAGGC GGAGCCAGCA GCAGAGGGGG GCGAGGGTGG 
GCGAGATTCA AGAGTGCCGT ATCTGTTGAA CCGGCGCTTC CTGCTGTTGA GCAGGAAAGG 
GACACGCAGA AAGCGGAGGA GTGTTCGAAA GCACCGCAGG CCTCAGAGGA GCCTGCAGCT 
TCTAAAAAGA GCCAGGAATC AGAAGGCGGC GGACAGACGG GGAGCACGGA GGGCGGCAAC 
ACAGCAGGAG ACGCGGCAGA GGAAACTAGC GCCCTGCACA AAACCGACTC CTGCGACAGC 
GGCATCACAA AGAGTGACCT GCGCATCGAC CGAGCTGGAG ACTCCCGGAG CTCCTTTGAG 
AGGAGCCCGA TGGAAAAGAG CCCCCTGGAC AGGAGTCCCT TCGAGCACAG CCTGGGGGTT 
TACACGGAGG TATCCCTGAG GCACTCCTTC CTCCAGCCGG TGTCTGAACA ATCCCTGCTG 
CAGATGACGC TGCACGAGGC CAAGCTGGAG CTGAAAGGAG ACATTAGGAA ACTGAGCAGT 
CGGCTGTCTC TCCTCGAGTC GCAAGTGAGC GAAGTCCTCA GGTTGCTGTC GTCCAAACGG 
AGACTCTCGC TGCCTCCGAC TTCTTCCAAG GCGAAAATAA AAAGTCACGA CGTGATGGCG 
TCCTCTAGGG TCGTGGCGCC AAAAGCAGAA GAGGAATCTT TGTGA