LOC110531014 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110531014
Entrez Gene ID	110531014
Full Name	DNA ligase 1-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021614292.1	XP_021469967.1	DNA ligase 1-like

Pathways from BioSystems
Gene Ontology

The following LOC110531014 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110531014 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf06340	XM_021614292.1 Latest version!	Oncorhynchus mykiss DNA ligase 1-like (LOC110531014), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110531014 ( XM_021614292.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021469967.1 Oncorhynchus mykiss DNA ligase 1-like (LOC110531014), mRNA.

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CloneID	OOf06340
Clone ID Related Accession (Same CDS sequence)	XM_021614292.1
Accession Version	XM_021614292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	DNA ligase 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035084.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGCAGCGAA GTATATCATC TTTCTTTCAG CCCAAAACGG GCAAAGAGAA GGAGAGCAAA 
AAAAGTGATG GACCGGGGAA AGACGAGCCG GTGAAAGACA AGCCAGAACC AATCAAAAGG 
CCTCTCAAAG TTCAGAATGG GGTCCATGAA GAGCCTGATT CGCCAGTGAA GAAGTTCACT 
AAACGCAGTC GTCAGATCTT GGACAGTGAT GATGAGGCTC CAGCCGGAAT AGAACAGGAG 
CCAGAGAACA AGGCTGTGCA ACCCAAGGAT GAAGTATTCA AGGCTGTTAC CAACCCCCTG 
TCCCCCGCCT CTCCAGTGAC CTCATCCGCC TCCCCCACCC CTGGAACCCC TGACACCCCC 
ACTTCCATCT CACCCTCCGG AATCCCCAAA CGCCGCACCG CAAGGAAGCA GTTCCCCAAG 
AGAAAGCTGG ACAGCAGGAG TGGCAGTGAA AAGGAGGAGG AAGAACAAGG ACAGCAGAAT 
AAAAGGGCAA AGGTTGAGGA GACGCAGGAC AGCACCGAGA ATGAAGAGGA AGCGATGGAC 
GTGGGAGGAG AGAACAGTGT GGATAAGCCT GAGGTGGCGC AGAACAACCC GAAGGAGAAA 
GAGACAGATG AAGAGAAGAC CAAAGATGGA GAGAAGGAGA AAGCACTTGA TGTGAAAGTG 
ACCGCGACCG AGGAGGAAAA AGGAAAAAGT GAAAAGGCTA AAGAGAGAAG TCCTAAAGAG 
AGAAGTCCTA AAGAGAGAAG TCCTAAAGAG AGAAGTTCGA AGGGTCAAAA GGTTAAAGAG 
ACAAGTCCTA AGGGTCAAAA GGTTAAAGAG AGAAGTCCTA AAGAGAGAAG TCAAAAGGTT 
AAAGAGACAA GTCCTAAGGG TCAAAAGGTT AAAGAGACAA GTCCTAAAGA GAGAAGTTTG 
AAGGGTCAAA AGGTTAAAGA GAGAAGTCCT AAGGGTCACA AGTCGGTAAA GGATGAGAAA 
GACATGAAAG AAGAGAAAGT GTCAGAAAGC CCCCAAGCCA AAAAAGCCCC CACCATCAGC 
AGTTTCTTTG CTCCCAGGAA GGCTGCTGTA AAGATGGAGA AACCAGAGAA GAGCGAGCGA 
GACGGAAAGG AGAAGAGCGA GGGAAAGGAG AAGAGCGAGG GAGAGGAGAA GAGCGAGGGA 
GAGGAGAAGA GCGAGGGAGA GGGGAAGAGC GAGGGAAAGG GGAAGAGCGA GGGAAAGGGG 
AGGAGCGAGG GAGACGGAAA GGAGAAGAGC GAGGGAAAGG AGAAGAGCGA GGGAAAGGGG 
AAGAGCGAGG GAAAGGGGAA GAGCGAGGGA AAGGGGAAGA GCGAGGGAAA GGGGAAGAGC 
GAGGGAAAGG GGAAGAGCGA GGAGTATGTA AAGGAAGAGG CGACGGTGTC CCGTACCACG 
GAGGACGTTA AAGACAAGAA AGGAGAGAAG ACTGCCCAGG CCCAGTATGA CCCGTCCAGA 
CCCAACTACC ACCCTGTAGA CCACGCCTGC TGGAGCCGGG GTCAGAGGGT CCCGTATTTG 
GCTGTTGCAC GCACCTTTGA AAAGATTGAG GAAGATTCTG GCAGATTGAG GAACATTGAA 
ACATTATCAA ATCTCCTACG CTCTGTTATC ATCCTCTCTC CAGAGGACCT GCTGTGCTGC 
GTCTACCTGT GTCTCAATCA GCTGGGTCCT GCTTACATGG GGCTGGAGCT GGGAGTGGGA 
GAAACTGTTC TGATGAAGGC TGTTGCCCAA GCTACTGGGC GACAGTTGGA CAAAATCAAG 
GCGGAGGCTG CAGAGAAGGG GGACTTGGGG TTGGTGGCTG AGAGTTCCCG TAACAACCAG 
AGAATTATGT TCCAGCCAGC CAATCTGATT GCAGGGGGTG TGTTCAGCAA ACTGAAAGAC 
ATCGGGAACA TGAGTGGGAA TTCTGCCATG AATAAGAAGA TTGACATCAT CAAAGGTCTC 
TTTGTAGCCT GTCGTTTCTC TGAAGCCAGA TACATTGTCA GGTCGCTGGC AGGGAAGCTG 
AGGATTGGCT TGGCTGAACA GAGTGTACTG TCTGCCCTGA GTCAGGCCGT GTGTCTGTCT 
CCTCCAGGAC AAGACTTCCC TCCGGCGGTG ATTGACGCAG GGAAAGGAAT GAGTGCAGAA 
AAACAGAAAG CTTGGATAGA AGAGAAGAGC CTTCTCCTCA AACAGACTTA CTGTGAGATG 
CCCAACTATG ATGTCATCAT CCCAGTCATT CTGAAGGAGG GGATTGATGA ACTCCCCAAC 
CACTGCAAAC TCACTCCAGG TGTACCGTTG AGGCCCATGC TGGCTCACCC CACCAAAGGT 
GTAGGAGAGG TGATGAAGAG ATTTGATGAA GCCGCCTTCA CCTGCGAGTA CAAGTATGAT 
GGAGAACGAG CACAGATCCA TATCTTGGAG AACGGGGAAG TGCGTATTTT CAGTCGCAAC 
CAGGAGGATA ATACCACCAA ATATCCTGAC ATCATCTCTC GCATTCATAC GGTAAAGAAT 
GAGTCTGTCA TATCCTGTGT GTTGGACACT GAGGCAGTAG CTTGGGACAG AGAGAAGAAA 
CAGATCCAAC CATTCCAGGT TCTCATCACA CGGAAGAGAA AGGTGACAGG AAAGAGAAGC 
AGACATGGTG CTATTGTGAC TCAGGCTGTG AGGCCCTCCC GAGTGGAGGC TGTAGAGAGC 
CCTGTGAGGC CCTCCCGAGT GGAGGCTGTA GAGAGCCCTC TGAAGAAGGA TTATCTGGAT 
GGCGTGGGGG ACACGATGGA TCTGTGTGTG ATCGGAGCGT ATCTGGGGAA GGGCAAGAGA 
GCAGGGACCT ACGGAGGCTT TCTGCTCGCC TGCTACGATG AGGACAATGA AGAGTTCCAG 
TCCGTCTGCA AGATTGGGAC AGGTTTCAAG GATGAAGATT TGGAGCAACA TTACAAATTC 
CTAAAGGAGC ATATTTTGCC AAAGCCCCGC CCCTATTACC GTGTGGACCA ATCAACTGAG 
CCCGACTTGT GGCTCGATGC CGTGCAAGTG TGGGAAGTGA AGTGCGCCGA CCTATCGCTG 
TCGCCGATCT ACAAGGCTGG CATGGGATTG TGTGACCCAG AGAAGGGCAT CTCTCTTCGG 
TTCCCACGCT TCCTGAGGAT CAGAGATGAT AAGAAGCCGG AGGAGGCCAC TTCAGGAGGA 
CAGATTGCGG ATTTATACAG GAAGCAGCAG GTTGTTCAGA ATCAGGGTGA CAAAGCTGAC 
CTCGAGGACT ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021469967.1
CDS	162..3359
Translation	MQRSISSFFQPKTGKEKESKKSDGPGKDEPVKDKPEPIKRPLKVQNGVHEEPDSPVKKFTKRSRQILDSDDEAPAGIEQEPENKAVQPKDEVFKAVTNPLSPASPVTSSASPTPGTPDTPTSISPSGIPKRRTARKQFPKRKLDSRSGSEKEEEEQGQQNKRAKVEETQDSTENEEEAMDVGGENSVDKPEVAQNNPKEKETDEEKTKDGEKEKALDVKVTATEEEKGKSEKAKERSPKERSPKERSPKERSSKGQKVKETSPKGQKVKERSPKERSQKVKETSPKGQKVKETSPKERSLKGQKVKERSPKGHKSVKDEKDMKEEKVSESPQAKKAPTISSFFAPRKAAVKMEKPEKSERDGKEKSEGKEKSEGEEKSEGEEKSEGEGKSEGKGKSEGKGRSEGDGKEKSEGKEKSEGKGKSEGKGKSEGKGKSEGKGKSEGKGKSEEYVKEEATVSRTTEDVKDKKGEKTAQAQYDPSRPNYHPVDHACWSRGQRVPYLAVARTFEKIEEDSGRLRNIETLSNLLRSVIILSPEDLLCCVYLCLNQLGPAYMGLELGVGETVLMKAVAQATGRQLDKIKAEAAEKGDLGLVAESSRNNQRIMFQPANLIAGGVFSKLKDIGNMSGNSAMNKKIDIIKGLFVACRFSEARYIVRSLAGKLRIGLAEQSVLSALSQAVCLSPPGQDFPPAVIDAGKGMSAEKQKAWIEEKSLLLKQTYCEMPNYDVIIPVILKEGIDELPNHCKLTPGVPLRPMLAHPTKGVGEVMKRFDEAAFTCEYKYDGERAQIHILENGEVRIFSRNQEDNTTKYPDIISRIHTVKNESVISCVLDTEAVAWDREKKQIQPFQVLITRKRKVTGKRSRHGAIVTQAVRPSRVEAVESPVRPSRVEAVESPLKKDYLDGVGDTMDLCVIGAYLGKGKRAGTYGGFLLACYDEDNEEFQSVCKIGTGFKDEDLEQHYKFLKEHILPKPRPYYRVDQSTEPDLWLDAVQVWEVKCADLSLSPIYKAGMGLCDPEKGISLRFPRFLRIRDDKKPEEATSGGQIADLYRKQQVVQNQGDKADLEDYY

Target ORF information:

RefSeq Version	XM_021614292.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss DNA ligase 1-like (LOC110531014), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021614292.1

ORF Insert Sequence:

ATGCAGCGAA GTATATCATC TTTCTTTCAG CCCAAAACGG GCAAAGAGAA GGAGAGCAAA 
AAAAGTGATG GACCGGGGAA AGACGAGCCG GTGAAAGACA AGCCAGAACC AATCAAAAGG 
CCTCTCAAAG TTCAGAATGG GGTCCATGAA GAGCCTGATT CGCCAGTGAA GAAGTTCACT 
AAACGCAGTC GTCAGATCTT GGACAGTGAT GATGAGGCTC CAGCCGGAAT AGAACAGGAG 
CCAGAGAACA AGGCTGTGCA ACCCAAGGAT GAAGTATTCA AGGCTGTTAC CAACCCCCTG 
TCCCCCGCCT CTCCAGTGAC CTCATCCGCC TCCCCCACCC CTGGAACCCC TGACACCCCC 
ACTTCCATCT CACCCTCCGG AATCCCCAAA CGCCGCACCG CAAGGAAGCA GTTCCCCAAG 
AGAAAGCTGG ACAGCAGGAG TGGCAGTGAA AAGGAGGAGG AAGAACAAGG ACAGCAGAAT 
AAAAGGGCAA AGGTTGAGGA GACGCAGGAC AGCACCGAGA ATGAAGAGGA AGCGATGGAC 
GTGGGAGGAG AGAACAGTGT GGATAAGCCT GAGGTGGCGC AGAACAACCC GAAGGAGAAA 
GAGACAGATG AAGAGAAGAC CAAAGATGGA GAGAAGGAGA AAGCACTTGA TGTGAAAGTG 
ACCGCGACCG AGGAGGAAAA AGGAAAAAGT GAAAAGGCTA AAGAGAGAAG TCCTAAAGAG 
AGAAGTCCTA AAGAGAGAAG TCCTAAAGAG AGAAGTTCGA AGGGTCAAAA GGTTAAAGAG 
ACAAGTCCTA AGGGTCAAAA GGTTAAAGAG AGAAGTCCTA AAGAGAGAAG TCAAAAGGTT 
AAAGAGACAA GTCCTAAGGG TCAAAAGGTT AAAGAGACAA GTCCTAAAGA GAGAAGTTTG 
AAGGGTCAAA AGGTTAAAGA GAGAAGTCCT AAGGGTCACA AGTCGGTAAA GGATGAGAAA 
GACATGAAAG AAGAGAAAGT GTCAGAAAGC CCCCAAGCCA AAAAAGCCCC CACCATCAGC 
AGTTTCTTTG CTCCCAGGAA GGCTGCTGTA AAGATGGAGA AACCAGAGAA GAGCGAGCGA 
GACGGAAAGG AGAAGAGCGA GGGAAAGGAG AAGAGCGAGG GAGAGGAGAA GAGCGAGGGA 
GAGGAGAAGA GCGAGGGAGA GGGGAAGAGC GAGGGAAAGG GGAAGAGCGA GGGAAAGGGG 
AGGAGCGAGG GAGACGGAAA GGAGAAGAGC GAGGGAAAGG AGAAGAGCGA GGGAAAGGGG 
AAGAGCGAGG GAAAGGGGAA GAGCGAGGGA AAGGGGAAGA GCGAGGGAAA GGGGAAGAGC 
GAGGGAAAGG GGAAGAGCGA GGAGTATGTA AAGGAAGAGG CGACGGTGTC CCGTACCACG 
GAGGACGTTA AAGACAAGAA AGGAGAGAAG ACTGCCCAGG CCCAGTATGA CCCGTCCAGA 
CCCAACTACC ACCCTGTAGA CCACGCCTGC TGGAGCCGGG GTCAGAGGGT CCCGTATTTG 
GCTGTTGCAC GCACCTTTGA AAAGATTGAG GAAGATTCTG GCAGATTGAG GAACATTGAA 
ACATTATCAA ATCTCCTACG CTCTGTTATC ATCCTCTCTC CAGAGGACCT GCTGTGCTGC 
GTCTACCTGT GTCTCAATCA GCTGGGTCCT GCTTACATGG GGCTGGAGCT GGGAGTGGGA 
GAAACTGTTC TGATGAAGGC TGTTGCCCAA GCTACTGGGC GACAGTTGGA CAAAATCAAG 
GCGGAGGCTG CAGAGAAGGG GGACTTGGGG TTGGTGGCTG AGAGTTCCCG TAACAACCAG 
AGAATTATGT TCCAGCCAGC CAATCTGATT GCAGGGGGTG TGTTCAGCAA ACTGAAAGAC 
ATCGGGAACA TGAGTGGGAA TTCTGCCATG AATAAGAAGA TTGACATCAT CAAAGGTCTC 
TTTGTAGCCT GTCGTTTCTC TGAAGCCAGA TACATTGTCA GGTCGCTGGC AGGGAAGCTG 
AGGATTGGCT TGGCTGAACA GAGTGTACTG TCTGCCCTGA GTCAGGCCGT GTGTCTGTCT 
CCTCCAGGAC AAGACTTCCC TCCGGCGGTG ATTGACGCAG GGAAAGGAAT GAGTGCAGAA 
AAACAGAAAG CTTGGATAGA AGAGAAGAGC CTTCTCCTCA AACAGACTTA CTGTGAGATG 
CCCAACTATG ATGTCATCAT CCCAGTCATT CTGAAGGAGG GGATTGATGA ACTCCCCAAC 
CACTGCAAAC TCACTCCAGG TGTACCGTTG AGGCCCATGC TGGCTCACCC CACCAAAGGT 
GTAGGAGAGG TGATGAAGAG ATTTGATGAA GCCGCCTTCA CCTGCGAGTA CAAGTATGAT 
GGAGAACGAG CACAGATCCA TATCTTGGAG AACGGGGAAG TGCGTATTTT CAGTCGCAAC 
CAGGAGGATA ATACCACCAA ATATCCTGAC ATCATCTCTC GCATTCATAC GGTAAAGAAT 
GAGTCTGTCA TATCCTGTGT GTTGGACACT GAGGCAGTAG CTTGGGACAG AGAGAAGAAA 
CAGATCCAAC CATTCCAGGT TCTCATCACA CGGAAGAGAA AGGTGACAGG AAAGAGAAGC 
AGACATGGTG CTATTGTGAC TCAGGCTGTG AGGCCCTCCC GAGTGGAGGC TGTAGAGAGC 
CCTGTGAGGC CCTCCCGAGT GGAGGCTGTA GAGAGCCCTC TGAAGAAGGA TTATCTGGAT 
GGCGTGGGGG ACACGATGGA TCTGTGTGTG ATCGGAGCGT ATCTGGGGAA GGGCAAGAGA 
GCAGGGACCT ACGGAGGCTT TCTGCTCGCC TGCTACGATG AGGACAATGA AGAGTTCCAG 
TCCGTCTGCA AGATTGGGAC AGGTTTCAAG GATGAAGATT TGGAGCAACA TTACAAATTC 
CTAAAGGAGC ATATTTTGCC AAAGCCCCGC CCCTATTACC GTGTGGACCA ATCAACTGAG 
CCCGACTTGT GGCTCGATGC CGTGCAAGTG TGGGAAGTGA AGTGCGCCGA CCTATCGCTG 
TCGCCGATCT ACAAGGCTGG CATGGGATTG TGTGACCCAG AGAAGGGCAT CTCTCTTCGG 
TTCCCACGCT TCCTGAGGAT CAGAGATGAT AAGAAGCCGG AGGAGGCCAC TTCAGGAGGA 
CAGATTGCGG ATTTATACAG GAAGCAGCAG GTTGTTCAGA ATCAGGGTGA CAAAGCTGAC 
CTCGAGGACT ACTACTGA