tysnd1 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	tysnd1
Entrez Gene ID	110530458
Full Name	trypsin domain containing 1
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021613537.1	XP_021469212.1	peroxisomal leader peptide-processing protease

Pathways from BioSystems
Gene Ontology

The following tysnd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tysnd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf53129	XM_021613537.1 Latest version!	Oncorhynchus mykiss trypsin domain containing 1 (tysnd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tysnd1 ( XM_021613537.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021469212.1 Oncorhynchus mykiss trypsin domain containing 1 (tysnd1), mRNA.

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CloneID	OOf53129
Clone ID Related Accession (Same CDS sequence)	XM_021613537.1
Accession Version	XM_021613537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	peroxisomal leader peptide-processing protease
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGA CTGAAAAAAT GTGTTGCGTG GTCACGGTGT CTGAGTCTTT TTCTGATCAG 
AAACCTGTAA GCTGTAGCGG AGTCATCCTG AACTCACAGA CTAGTCTCGT TCTCTGCAAT 
GGTCTTATCT TTTCTCGCTT TATGAATGAC AAGGACGCTT TCTCTTCAGA TCACCGGGTC 
TTGCTACCAG ACAGTTTCAG TGGAAAACTC AAAATTAGCC TTAACATCCA TGAACAGCGA 
GATTTGGACA CAAACCAATG CGCATACCAG TCATGCACCG TCGCGGAGAG CAACAGCACC 
TCGCGGCATC AACACCAAGC GACAGCACAG CTGCTGATGC TCGCTAACTG TTTGGAGTTC 
AAGAGTGCTT TCGAGAAGAT CTTCAAAGAG TCTGATAAGT GGAGTTTTTA CGGTGGGGAG 
GAGGATGATG ATGCAGAGAT GGTTAGGGAT TCAGAGTTTC TGAGCTGGTT TGCTGTACTC 
AAGGTACCAG CCTTGGCCAA AGCATCAAAC ATCAACTTTG GAACCATACC ATGGGCGACC 
AGCTCCACTC TCCGGAAAGG ATGCGCGGTC CTCGCCTGCG GCTCTCCCTT CGGCGCCTTC 
TGCCCCGATC TCTTCATGGG CACACTAAGC AAGGGTATCA TCAGTAACCT GTCCGGGGAA 
GGTAATGCTC TCATTCTCAC TGATGCCCGC TGCCTGCCCG GTACAGAGGG AGGGGGGCTT 
TTTGTGCCCC ACGGAGACCG AACGCTCCTC GTCGGTTTGA TCGTGTCCCC CTTGTGCTGG 
AAAGCCAGCG AGTGGATTGG GCTGACACTT GTGTGCTCGC TCCAGATGAT TCTGAGGAAC 
ATCACACAGT GTGTGAATAT TTGCAATCCA CACCAAGAGA TCCACCACAT GCAGGAGCCC 
ACAGACTTCT TTCTAGCCTT ACAGAGTGAA ACTGAAACTC AGACGTACCC CACAGTGGCC 
TTAGTAGACG CTGGACAATT CTGGGGCTCT GGGGTTCTGG TTAGTCCGCG GTTGGTGGTC 
ACCTGTCGAC ATGTTGTCAA TGGAAAACCT GTTGTCACCT TGAGATTCAA CACCAATGAC 
AGATTTCATG CCGTGGTGAG TGATGTCCTG TACTCCACCA AGGCATCGTC TCCATATGAT 
GTAGCCGTGG TGCAGCTAAG ACACCTTCCC CCGGAGGTAG TTGTGTCACG CTTGGCCACA 
TCGTTTACAC CAGGTGAGGA CGTGGTGGTG GTGGGTTACG GAGCTTTCGG CCAGCGTTGT 
GGTCCCTCTC TGACCAGTGG CATCCTGTCC AGGGCGATCG GCTGTCATGG CCAGGCTGTC 
ATGCTGCAGA CAACCGCTGC AGTGCAGGCA GGGGCCAGCG GAGGAGCTGT GGTGCGGGCA 
GGATCAGGAG AGCTGCTAGG TCTGGTTTCC AGTAACACCA GAGACTTTTC TGCCAAGGTA 
ACCTACCCTC ACCTGAACTT CAGTGTCCCA ATGACTGTCT TGGAACCGTT GCTACGGCGA 
TACGCCCAGA AGAGCGACAT TGGTGTGTTC CGGGACTTGG ATACGACCGA TGAGGGAGTC 
AGGAGGGTGT GGAGGCTACA GACTGCTCAG AGCAAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021469212.1
CDS	392..1993
Translation	MESTEKMCCVVTVSESFSDQKPVSCSGVILNSQTSLVLCNGLIFSRFMNDKDAFSSDHRVLLPDSFSGKLKISLNIHEQRDLDTNQCAYQSCTVAESNSTSRHQHQATAQLLMLANCLEFKSAFEKIFKESDKWSFYGGEEDDDAEMVRDSEFLSWFAVLKVPALAKASNINFGTIPWATSSTLRKGCAVLACGSPFGAFCPDLFMGTLSKGIISNLSGEGNALILTDARCLPGTEGGGLFVPHGDRTLLVGLIVSPLCWKASEWIGLTLVCSLQMILRNITQCVNICNPHQEIHHMQEPTDFFLALQSETETQTYPTVALVDAGQFWGSGVLVSPRLVVTCRHVVNGKPVVTLRFNTNDRFHAVVSDVLYSTKASSPYDVAVVQLRHLPPEVVVSRLATSFTPGEDVVVVGYGAFGQRCGPSLTSGILSRAIGCHGQAVMLQTTAAVQAGASGGAVVRAGSGELLGLVSSNTRDFSAKVTYPHLNFSVPMTVLEPLLRRYAQKSDIGVFRDLDTTDEGVRRVWRLQTAQSKL

Target ORF information:

RefSeq Version	XM_021613537.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss trypsin domain containing 1 (tysnd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021613537.1

ORF Insert Sequence:

ATGGAGTCGA CTGAAAAAAT GTGTTGCGTG GTCACGGTGT CTGAGTCTTT TTCTGATCAG 
AAACCTGTAA GCTGTAGCGG AGTCATCCTG AACTCACAGA CTAGTCTCGT TCTCTGCAAT 
GGTCTTATCT TTTCTCGCTT TATGAATGAC AAGGACGCTT TCTCTTCAGA TCACCGGGTC 
TTGCTACCAG ACAGTTTCAG TGGAAAACTC AAAATTAGCC TTAACATCCA TGAACAGCGA 
GATTTGGACA CAAACCAATG CGCATACCAG TCATGCACCG TCGCGGAGAG CAACAGCACC 
TCGCGGCATC AACACCAAGC GACAGCACAG CTGCTGATGC TCGCTAACTG TTTGGAGTTC 
AAGAGTGCTT TCGAGAAGAT CTTCAAAGAG TCTGATAAGT GGAGTTTTTA CGGTGGGGAG 
GAGGATGATG ATGCAGAGAT GGTTAGGGAT TCAGAGTTTC TGAGCTGGTT TGCTGTACTC 
AAGGTACCAG CCTTGGCCAA AGCATCAAAC ATCAACTTTG GAACCATACC ATGGGCGACC 
AGCTCCACTC TCCGGAAAGG ATGCGCGGTC CTCGCCTGCG GCTCTCCCTT CGGCGCCTTC 
TGCCCCGATC TCTTCATGGG CACACTAAGC AAGGGTATCA TCAGTAACCT GTCCGGGGAA 
GGTAATGCTC TCATTCTCAC TGATGCCCGC TGCCTGCCCG GTACAGAGGG AGGGGGGCTT 
TTTGTGCCCC ACGGAGACCG AACGCTCCTC GTCGGTTTGA TCGTGTCCCC CTTGTGCTGG 
AAAGCCAGCG AGTGGATTGG GCTGACACTT GTGTGCTCGC TCCAGATGAT TCTGAGGAAC 
ATCACACAGT GTGTGAATAT TTGCAATCCA CACCAAGAGA TCCACCACAT GCAGGAGCCC 
ACAGACTTCT TTCTAGCCTT ACAGAGTGAA ACTGAAACTC AGACGTACCC CACAGTGGCC 
TTAGTAGACG CTGGACAATT CTGGGGCTCT GGGGTTCTGG TTAGTCCGCG GTTGGTGGTC 
ACCTGTCGAC ATGTTGTCAA TGGAAAACCT GTTGTCACCT TGAGATTCAA CACCAATGAC 
AGATTTCATG CCGTGGTGAG TGATGTCCTG TACTCCACCA AGGCATCGTC TCCATATGAT 
GTAGCCGTGG TGCAGCTAAG ACACCTTCCC CCGGAGGTAG TTGTGTCACG CTTGGCCACA 
TCGTTTACAC CAGGTGAGGA CGTGGTGGTG GTGGGTTACG GAGCTTTCGG CCAGCGTTGT 
GGTCCCTCTC TGACCAGTGG CATCCTGTCC AGGGCGATCG GCTGTCATGG CCAGGCTGTC 
ATGCTGCAGA CAACCGCTGC AGTGCAGGCA GGGGCCAGCG GAGGAGCTGT GGTGCGGGCA 
GGATCAGGAG AGCTGCTAGG TCTGGTTTCC AGTAACACCA GAGACTTTTC TGCCAAGGTA 
ACCTACCCTC ACCTGAACTT CAGTGTCCCA ATGACTGTCT TGGAACCGTT GCTACGGCGA 
TACGCCCAGA AGAGCGACAT TGGTGTGTTC CGGGACTTGG ATACGACCGA TGAGGGAGTC 
AGGAGGGTGT GGAGGCTACA GACTGCTCAG AGCAAACTGT GA