LOC110496290 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110496290
Entrez Gene ID	110496290
Full Name	KN motif and ankyrin repeat domain-containing protein 2-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021572139.1	XP_021427814.1	KN motif and ankyrin repeat domain-containing protein 2-like
XM_021572213.1	XP_021427888.1	KN motif and ankyrin repeat domain-containing protein 2-like

Pathways from BioSystems
Gene Ontology

The following LOC110496290 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110496290 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf51483	XM_021572139.1 Latest version!	Oncorhynchus mykiss KN motif and ankyrin repeat domain-containing protein 2-like (LOC110496290), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOf51483	XM_021572213.1 Latest version!	Oncorhynchus mykiss KN motif and ankyrin repeat domain-containing protein 2-like (LOC110496290), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110496290 ( XM_021572139.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427814.1 Oncorhynchus mykiss KN motif and ankyrin repeat domain-containing protein 2-like (LOC110496290), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf51483
Clone ID Related Accession (Same CDS sequence)	XM_021572139.1 , XM_021572213.1
Accession Version	XM_021572139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	KN motif and ankyrin repeat domain-containing protein 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAGT CTGTTCAGGT CAACCCAAAG CTGCCTGATC TGGGCGCACC GTTTCTATAC 
TCCAGTCAGG AAGAGGCGGA TCAACAGGGC TCTTATTCGG TTCAGACACC GTATGGCTTC 
CAGCTGGACC TGGACTTCCT CAAGTATGTG GAGGAAATAG AAAGCGGCCA CCACGTCCGA 
CGGGCACCCG TCAACTCCCG CAGGTCATCC CGGGGGGTCA AAGTCTCCCA GCGGAGCCCG 
AGTGTGGGGG GACGCGCCAG CGGCTGGACC TCCACAGAGT CCCTCTCCTC GCCCGCCAGC 
GAAGATGGTC GAGTGCCCCC TCCCCCGCCC CCACGGAATC GCATTGGCTC TGCTCCCAGT 
GAGGGGCAAC TCCTGTCCTC GCTATCTGTC CTCAGCCCTC CTCTCTCTGC CGGCGCCAAA 
GTGCCACCAC CACCTCCACT ACGTAACTCC AGAGTAGAGA GGACCCTCCT GGAGACCAGC 
CTGAGACTGC AGCAGGAGCA GAGCCATCAG CACCAAAATG GCACCCGTTT CCAGCTCTCA 
GACCCACCAA AGCAAAACCC CAGGACTGCC CCTACCCATG TCACCCCAGC TAGTGCAGCA 
GCCAGCGTGG ATGGACAGCT CTTGCACCTG TCCTCTGCCT CTCCCAGCCC ATCTCAGAGT 
AGCCTGCACA GACCCAGTCC CCACACCTCC GGTAAGAGCA CCCCGGCCTC TAGCGCCGGA 
ATGGCTACTC TGCCCCCCAG CCAATTGCAG ACGGTGAGAG AACAGATGGC CACTGCCCTG 
AAGCAACTGA AGGAGATGGA GGAGCGAGTA AAGGGTGTCC CAGTGCTGGA AAGAGAGGTG 
GCCAAGCTAC AGGCTGAGAA AGACATGCTC CTACTGGCAC TGCAGGAGAA GAAGACCTTG 
GTGGCCCAAC AACAGGCTAC AACAGTAGTC AGCAGTACTA ATACCACCAC ATTGGACGCA 
GGCACATGGA GTCAGGAACG TCCTATTTTA TCCCCTAAGT GTCCCAGATG TCCAAAGAGT 
CCCTGTAAAT TAGGGGACCT CAGAAAGCTG ACAGAGAAGT TCGAAGGCAA GACAGAAAAA 
GATGGAGCAC GTTCTGAGAA AGTTCCGGAA AAGGTTGTGG AGAAGAAGTC TGTGGCCATC 
GGGGACGACA TGTTGCTGGA TGCTGGTGTC TTCTACTACA GCCTGGGCAC CAAGGACGCC 
TCAGAGGGCA CGCTGCAGAT GGACGTCTGC GAGAAAGGAA TGGCCACGGA GGCGCCGGCC 
ATCCACGAGG AGGGGGTGCA GGCTGTAGTG GAGACAGAGG AGGCCTCAGT TTGGGTGATG 
GAATCCCAGC TGGGCCTAAG CAGCGATGCC CAGAGGGAGA TCGACACCCT ACAGGACAAC 
ATCAAGTTCC AGCAGGAGTC CATCTCGGTT CTGGAGGGGC GACTTGGCCA GGCTGACGAG 
GTCCTGGCGG TGCTCAAAGC CCAGGAGAAT GAAAGGAAGT CCAAGGCAAC GTCCGACAAG 
GGGGTCCTTG TCAAACCAGA CTCTGCCCAT GCCCAAGTGG GGACTGAAGC CTCTACAATG 
TCCGCACCAG CTTCACAGAA TGTCGCAGTC TCCTGTTGTC CTGAGGTGGT TGACGCATGT 
GTAGGTGAGG ATTTGAGAGC CGGATATTAC GACCAGAGCA CTCAGACTGA CTCTGTGGAG 
ATCCCTGCAG AAGCGGAATC AACCCCAGTA GTACTGGTCA GCACTGGAAG TCAATGGGAG 
AATCTGTACA TGGAGGAGGA CATAGAGCAG AAAGCTCCAG TCACTGCGCT GAAGAGGAGA 
CAGACGACCA TCGCGGAGTA CAAGGTCAAC CCTGGCAAGG AGACGGTCAG TTTACCCAAA 
GGAAAGGGAG TGAAAGAGGA GGAAATGGCG CCCAGCACTC CAACAACTGC AACAACGGTT 
GGAAGTATGT TGAAATCAAT CATGAAGAAG AAGGATGGGA GTAGTTCCAG TGAATCACGC 
AGCAGCGGCA AGAAAAGCTT GCAGTTTGTT GGCATTCTCA ACGGGGGGTA TGAGTCGACA 
TCTAGTGAAG AGGAGGAGGA TGATGATGAG GAAGAGGGGA GTTCCTCAGG TGGCAGTGAA 
GTAGGTGATT GCTCAGACGG TAGTGATGAT GAAGATGCAG CGGCCGCTCT GGAGGAGACC 
TCCGATGAGG AGAGGAGCAT GAATATGGAT GACACCGATA GTGATGATGT GGCCTTAGCA 
GCAGGGACTG GAGCCACTGA GGAGATTCCA GATAAAGTGA AAGAGAAGTT TGAGCTGAGT 
GCAAAAATGC GTGAAGCTTG TCTCATTTTG AAGAACCATC TGAATGATGA TGTCAAAACA 
CTGAAGAGTA AAGAAGTGAT CTCCAGCACT CACACTGTGC AGCTGGAATG GTTCCGCATC 
TCCAGTGCCA AGCTGGCCCA GCAGCCCCGT GTCTCAGACT ACCTGATGGC CTTCACAGAA 
GTCTCCTCTG CCTTGCTGGC CCACGTCGTC AACATTACCG ATGGCAATGG CAACACAGCC 
TTGCACTACA GCGTCTCCCA CTCCAACTTC ACCGTGGTGG GGCTACTGCT GGACACAGGC 
ATGTGTTGTG TAGATAAGCA GAACAAGGCA GGCTACACTG CGATCATGCT GGCGGCCCTC 
TCAGCTGTGA AAGAGGAGGA TGACATGGTG GTGGTCAGGA AGCTCTTCAG TCAGGGCAAC 
GTCAACGCCA AGGCCAGCCA GGCTGGCCAG ACGGGGCTGA TGCTAGCTGT TAGCCATGGA 
CGGCAGGAGA TGGTGCGGGC ATTGCTGGAC TGTGGCGCTG ACGTGAACGT GCAGGATGAC 
GAGGGCTCCA CGGCGCTCAT GTGCGCCAGC GAGCACGGCC GCGCCGAGAT TGTCTCGCTG 
CTCCTGGACC AGCCGGGCTG TGACATCTCC ATTGTGGACA ATGACGGCAG TAATGCTCTG 
TCCATCGCGC TGGAGGCCTC TCACAATGAC ACAGCTGTGC TGCTTTACGC CCACATGAAC 
TACGCCAAAG CCCAGACAGC TGGGACAACC AAGACTCGAA GCCCCACTAG TCCTCAAAAG 
ACATGGCCTG CTGCGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427814.1
CDS	181..3261
Translation	MTQSVQVNPKLPDLGAPFLYSSQEEADQQGSYSVQTPYGFQLDLDFLKYVEEIESGHHVRRAPVNSRRSSRGVKVSQRSPSVGGRASGWTSTESLSSPASEDGRVPPPPPPRNRIGSAPSEGQLLSSLSVLSPPLSAGAKVPPPPPLRNSRVERTLLETSLRLQQEQSHQHQNGTRFQLSDPPKQNPRTAPTHVTPASAAASVDGQLLHLSSASPSPSQSSLHRPSPHTSGKSTPASSAGMATLPPSQLQTVREQMATALKQLKEMEERVKGVPVLEREVAKLQAEKDMLLLALQEKKTLVAQQQATTVVSSTNTTTLDAGTWSQERPILSPKCPRCPKSPCKLGDLRKLTEKFEGKTEKDGARSEKVPEKVVEKKSVAIGDDMLLDAGVFYYSLGTKDASEGTLQMDVCEKGMATEAPAIHEEGVQAVVETEEASVWVMESQLGLSSDAQREIDTLQDNIKFQQESISVLEGRLGQADEVLAVLKAQENERKSKATSDKGVLVKPDSAHAQVGTEASTMSAPASQNVAVSCCPEVVDACVGEDLRAGYYDQSTQTDSVEIPAEAESTPVVLVSTGSQWENLYMEEDIEQKAPVTALKRRQTTIAEYKVNPGKETVSLPKGKGVKEEEMAPSTPTTATTVGSMLKSIMKKKDGSSSSESRSSGKKSLQFVGILNGGYESTSSEEEEDDDEEEGSSSGGSEVGDCSDGSDDEDAAAALEETSDEERSMNMDDTDSDDVALAAGTGATEEIPDKVKEKFELSAKMREACLILKNHLNDDVKTLKSKEVISSTHTVQLEWFRISSAKLAQQPRVSDYLMAFTEVSSALLAHVVNITDGNGNTALHYSVSHSNFTVVGLLLDTGMCCVDKQNKAGYTAIMLAALSAVKEEDDMVVVRKLFSQGNVNAKASQAGQTGLMLAVSHGRQEMVRALLDCGADVNVQDDEGSTALMCASEHGRAEIVSLLLDQPGCDISIVDNDGSNALSIALEASHNDTAVLLYAHMNYAKAQTAGTTKTRSPTSPQKTWPAAE

Target ORF information:

RefSeq Version	XM_021572139.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss KN motif and ankyrin repeat domain-containing protein 2-like (LOC110496290), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021572139.1

ORF Insert Sequence:

ATGACTCAGT CTGTTCAGGT CAACCCAAAG CTGCCTGATC TGGGCGCACC GTTTCTATAC 
TCCAGTCAGG AAGAGGCGGA TCAACAGGGC TCTTATTCGG TTCAGACACC GTATGGCTTC 
CAGCTGGACC TGGACTTCCT CAAGTATGTG GAGGAAATAG AAAGCGGCCA CCACGTCCGA 
CGGGCACCCG TCAACTCCCG CAGGTCATCC CGGGGGGTCA AAGTCTCCCA GCGGAGCCCG 
AGTGTGGGGG GACGCGCCAG CGGCTGGACC TCCACAGAGT CCCTCTCCTC GCCCGCCAGC 
GAAGATGGTC GAGTGCCCCC TCCCCCGCCC CCACGGAATC GCATTGGCTC TGCTCCCAGT 
GAGGGGCAAC TCCTGTCCTC GCTATCTGTC CTCAGCCCTC CTCTCTCTGC CGGCGCCAAA 
GTGCCACCAC CACCTCCACT ACGTAACTCC AGAGTAGAGA GGACCCTCCT GGAGACCAGC 
CTGAGACTGC AGCAGGAGCA GAGCCATCAG CACCAAAATG GCACCCGTTT CCAGCTCTCA 
GACCCACCAA AGCAAAACCC CAGGACTGCC CCTACCCATG TCACCCCAGC TAGTGCAGCA 
GCCAGCGTGG ATGGACAGCT CTTGCACCTG TCCTCTGCCT CTCCCAGCCC ATCTCAGAGT 
AGCCTGCACA GACCCAGTCC CCACACCTCC GGTAAGAGCA CCCCGGCCTC TAGCGCCGGA 
ATGGCTACTC TGCCCCCCAG CCAATTGCAG ACGGTGAGAG AACAGATGGC CACTGCCCTG 
AAGCAACTGA AGGAGATGGA GGAGCGAGTA AAGGGTGTCC CAGTGCTGGA AAGAGAGGTG 
GCCAAGCTAC AGGCTGAGAA AGACATGCTC CTACTGGCAC TGCAGGAGAA GAAGACCTTG 
GTGGCCCAAC AACAGGCTAC AACAGTAGTC AGCAGTACTA ATACCACCAC ATTGGACGCA 
GGCACATGGA GTCAGGAACG TCCTATTTTA TCCCCTAAGT GTCCCAGATG TCCAAAGAGT 
CCCTGTAAAT TAGGGGACCT CAGAAAGCTG ACAGAGAAGT TCGAAGGCAA GACAGAAAAA 
GATGGAGCAC GTTCTGAGAA AGTTCCGGAA AAGGTTGTGG AGAAGAAGTC TGTGGCCATC 
GGGGACGACA TGTTGCTGGA TGCTGGTGTC TTCTACTACA GCCTGGGCAC CAAGGACGCC 
TCAGAGGGCA CGCTGCAGAT GGACGTCTGC GAGAAAGGAA TGGCCACGGA GGCGCCGGCC 
ATCCACGAGG AGGGGGTGCA GGCTGTAGTG GAGACAGAGG AGGCCTCAGT TTGGGTGATG 
GAATCCCAGC TGGGCCTAAG CAGCGATGCC CAGAGGGAGA TCGACACCCT ACAGGACAAC 
ATCAAGTTCC AGCAGGAGTC CATCTCGGTT CTGGAGGGGC GACTTGGCCA GGCTGACGAG 
GTCCTGGCGG TGCTCAAAGC CCAGGAGAAT GAAAGGAAGT CCAAGGCAAC GTCCGACAAG 
GGGGTCCTTG TCAAACCAGA CTCTGCCCAT GCCCAAGTGG GGACTGAAGC CTCTACAATG 
TCCGCACCAG CTTCACAGAA TGTCGCAGTC TCCTGTTGTC CTGAGGTGGT TGACGCATGT 
GTAGGTGAGG ATTTGAGAGC CGGATATTAC GACCAGAGCA CTCAGACTGA CTCTGTGGAG 
ATCCCTGCAG AAGCGGAATC AACCCCAGTA GTACTGGTCA GCACTGGAAG TCAATGGGAG 
AATCTGTACA TGGAGGAGGA CATAGAGCAG AAAGCTCCAG TCACTGCGCT GAAGAGGAGA 
CAGACGACCA TCGCGGAGTA CAAGGTCAAC CCTGGCAAGG AGACGGTCAG TTTACCCAAA 
GGAAAGGGAG TGAAAGAGGA GGAAATGGCG CCCAGCACTC CAACAACTGC AACAACGGTT 
GGAAGTATGT TGAAATCAAT CATGAAGAAG AAGGATGGGA GTAGTTCCAG TGAATCACGC 
AGCAGCGGCA AGAAAAGCTT GCAGTTTGTT GGCATTCTCA ACGGGGGGTA TGAGTCGACA 
TCTAGTGAAG AGGAGGAGGA TGATGATGAG GAAGAGGGGA GTTCCTCAGG TGGCAGTGAA 
GTAGGTGATT GCTCAGACGG TAGTGATGAT GAAGATGCAG CGGCCGCTCT GGAGGAGACC 
TCCGATGAGG AGAGGAGCAT GAATATGGAT GACACCGATA GTGATGATGT GGCCTTAGCA 
GCAGGGACTG GAGCCACTGA GGAGATTCCA GATAAAGTGA AAGAGAAGTT TGAGCTGAGT 
GCAAAAATGC GTGAAGCTTG TCTCATTTTG AAGAACCATC TGAATGATGA TGTCAAAACA 
CTGAAGAGTA AAGAAGTGAT CTCCAGCACT CACACTGTGC AGCTGGAATG GTTCCGCATC 
TCCAGTGCCA AGCTGGCCCA GCAGCCCCGT GTCTCAGACT ACCTGATGGC CTTCACAGAA 
GTCTCCTCTG CCTTGCTGGC CCACGTCGTC AACATTACCG ATGGCAATGG CAACACAGCC 
TTGCACTACA GCGTCTCCCA CTCCAACTTC ACCGTGGTGG GGCTACTGCT GGACACAGGC 
ATGTGTTGTG TAGATAAGCA GAACAAGGCA GGCTACACTG CGATCATGCT GGCGGCCCTC 
TCAGCTGTGA AAGAGGAGGA TGACATGGTG GTGGTCAGGA AGCTCTTCAG TCAGGGCAAC 
GTCAACGCCA AGGCCAGCCA GGCTGGCCAG ACGGGGCTGA TGCTAGCTGT TAGCCATGGA 
CGGCAGGAGA TGGTGCGGGC ATTGCTGGAC TGTGGCGCTG ACGTGAACGT GCAGGATGAC 
GAGGGCTCCA CGGCGCTCAT GTGCGCCAGC GAGCACGGCC GCGCCGAGAT TGTCTCGCTG 
CTCCTGGACC AGCCGGGCTG TGACATCTCC ATTGTGGACA ATGACGGCAG TAATGCTCTG 
TCCATCGCGC TGGAGGCCTC TCACAATGAC ACAGCTGTGC TGCTTTACGC CCACATGAAC 
TACGCCAAAG CCCAGACAGC TGGGACAACC AAGACTCGAA GCCCCACTAG TCCTCAAAAG 
ACATGGCCTG CTGCGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110496290 ( XM_021572213.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021427888.1 Oncorhynchus mykiss KN motif and ankyrin repeat domain-containing protein 2-like (LOC110496290), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf51483
Clone ID Related Accession (Same CDS sequence)	XM_021572139.1 , XM_021572213.1
Accession Version	XM_021572213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	KN motif and ankyrin repeat domain-containing protein 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035077.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAGT CTGTTCAGGT CAACCCAAAG CTGCCTGATC TGGGCGCACC GTTTCTATAC 
TCCAGTCAGG AAGAGGCGGA TCAACAGGGC TCTTATTCGG TTCAGACACC GTATGGCTTC 
CAGCTGGACC TGGACTTCCT CAAGTATGTG GAGGAAATAG AAAGCGGCCA CCACGTCCGA 
CGGGCACCCG TCAACTCCCG CAGGTCATCC CGGGGGGTCA AAGTCTCCCA GCGGAGCCCG 
AGTGTGGGGG GACGCGCCAG CGGCTGGACC TCCACAGAGT CCCTCTCCTC GCCCGCCAGC 
GAAGATGGTC GAGTGCCCCC TCCCCCGCCC CCACGGAATC GCATTGGCTC TGCTCCCAGT 
GAGGGGCAAC TCCTGTCCTC GCTATCTGTC CTCAGCCCTC CTCTCTCTGC CGGCGCCAAA 
GTGCCACCAC CACCTCCACT ACGTAACTCC AGAGTAGAGA GGACCCTCCT GGAGACCAGC 
CTGAGACTGC AGCAGGAGCA GAGCCATCAG CACCAAAATG GCACCCGTTT CCAGCTCTCA 
GACCCACCAA AGCAAAACCC CAGGACTGCC CCTACCCATG TCACCCCAGC TAGTGCAGCA 
GCCAGCGTGG ATGGACAGCT CTTGCACCTG TCCTCTGCCT CTCCCAGCCC ATCTCAGAGT 
AGCCTGCACA GACCCAGTCC CCACACCTCC GGTAAGAGCA CCCCGGCCTC TAGCGCCGGA 
ATGGCTACTC TGCCCCCCAG CCAATTGCAG ACGGTGAGAG AACAGATGGC CACTGCCCTG 
AAGCAACTGA AGGAGATGGA GGAGCGAGTA AAGGGTGTCC CAGTGCTGGA AAGAGAGGTG 
GCCAAGCTAC AGGCTGAGAA AGACATGCTC CTACTGGCAC TGCAGGAGAA GAAGACCTTG 
GTGGCCCAAC AACAGGCTAC AACAGTAGTC AGCAGTACTA ATACCACCAC ATTGGACGCA 
GGCACATGGA GTCAGGAACG TCCTATTTTA TCCCCTAAGT GTCCCAGATG TCCAAAGAGT 
CCCTGTAAAT TAGGGGACCT CAGAAAGCTG ACAGAGAAGT TCGAAGGCAA GACAGAAAAA 
GATGGAGCAC GTTCTGAGAA AGTTCCGGAA AAGGTTGTGG AGAAGAAGTC TGTGGCCATC 
GGGGACGACA TGTTGCTGGA TGCTGGTGTC TTCTACTACA GCCTGGGCAC CAAGGACGCC 
TCAGAGGGCA CGCTGCAGAT GGACGTCTGC GAGAAAGGAA TGGCCACGGA GGCGCCGGCC 
ATCCACGAGG AGGGGGTGCA GGCTGTAGTG GAGACAGAGG AGGCCTCAGT TTGGGTGATG 
GAATCCCAGC TGGGCCTAAG CAGCGATGCC CAGAGGGAGA TCGACACCCT ACAGGACAAC 
ATCAAGTTCC AGCAGGAGTC CATCTCGGTT CTGGAGGGGC GACTTGGCCA GGCTGACGAG 
GTCCTGGCGG TGCTCAAAGC CCAGGAGAAT GAAAGGAAGT CCAAGGCAAC GTCCGACAAG 
GGGGTCCTTG TCAAACCAGA CTCTGCCCAT GCCCAAGTGG GGACTGAAGC CTCTACAATG 
TCCGCACCAG CTTCACAGAA TGTCGCAGTC TCCTGTTGTC CTGAGGTGGT TGACGCATGT 
GTAGGTGAGG ATTTGAGAGC CGGATATTAC GACCAGAGCA CTCAGACTGA CTCTGTGGAG 
ATCCCTGCAG AAGCGGAATC AACCCCAGTA GTACTGGTCA GCACTGGAAG TCAATGGGAG 
AATCTGTACA TGGAGGAGGA CATAGAGCAG AAAGCTCCAG TCACTGCGCT GAAGAGGAGA 
CAGACGACCA TCGCGGAGTA CAAGGTCAAC CCTGGCAAGG AGACGGTCAG TTTACCCAAA 
GGAAAGGGAG TGAAAGAGGA GGAAATGGCG CCCAGCACTC CAACAACTGC AACAACGGTT 
GGAAGTATGT TGAAATCAAT CATGAAGAAG AAGGATGGGA GTAGTTCCAG TGAATCACGC 
AGCAGCGGCA AGAAAAGCTT GCAGTTTGTT GGCATTCTCA ACGGGGGGTA TGAGTCGACA 
TCTAGTGAAG AGGAGGAGGA TGATGATGAG GAAGAGGGGA GTTCCTCAGG TGGCAGTGAA 
GTAGGTGATT GCTCAGACGG TAGTGATGAT GAAGATGCAG CGGCCGCTCT GGAGGAGACC 
TCCGATGAGG AGAGGAGCAT GAATATGGAT GACACCGATA GTGATGATGT GGCCTTAGCA 
GCAGGGACTG GAGCCACTGA GGAGATTCCA GATAAAGTGA AAGAGAAGTT TGAGCTGAGT 
GCAAAAATGC GTGAAGCTTG TCTCATTTTG AAGAACCATC TGAATGATGA TGTCAAAACA 
CTGAAGAGTA AAGAAGTGAT CTCCAGCACT CACACTGTGC AGCTGGAATG GTTCCGCATC 
TCCAGTGCCA AGCTGGCCCA GCAGCCCCGT GTCTCAGACT ACCTGATGGC CTTCACAGAA 
GTCTCCTCTG CCTTGCTGGC CCACGTCGTC AACATTACCG ATGGCAATGG CAACACAGCC 
TTGCACTACA GCGTCTCCCA CTCCAACTTC ACCGTGGTGG GGCTACTGCT GGACACAGGC 
ATGTGTTGTG TAGATAAGCA GAACAAGGCA GGCTACACTG CGATCATGCT GGCGGCCCTC 
TCAGCTGTGA AAGAGGAGGA TGACATGGTG GTGGTCAGGA AGCTCTTCAG TCAGGGCAAC 
GTCAACGCCA AGGCCAGCCA GGCTGGCCAG ACGGGGCTGA TGCTAGCTGT TAGCCATGGA 
CGGCAGGAGA TGGTGCGGGC ATTGCTGGAC TGTGGCGCTG ACGTGAACGT GCAGGATGAC 
GAGGGCTCCA CGGCGCTCAT GTGCGCCAGC GAGCACGGCC GCGCCGAGAT TGTCTCGCTG 
CTCCTGGACC AGCCGGGCTG TGACATCTCC ATTGTGGACA ATGACGGCAG TAATGCTCTG 
TCCATCGCGC TGGAGGCCTC TCACAATGAC ACAGCTGTGC TGCTTTACGC CCACATGAAC 
TACGCCAAAG CCCAGACAGC TGGGACAACC AAGACTCGAA GCCCCACTAG TCCTCAAAAG 
ACATGGCCTG CTGCGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021427888.1
CDS	198..3278
Translation	MTQSVQVNPKLPDLGAPFLYSSQEEADQQGSYSVQTPYGFQLDLDFLKYVEEIESGHHVRRAPVNSRRSSRGVKVSQRSPSVGGRASGWTSTESLSSPASEDGRVPPPPPPRNRIGSAPSEGQLLSSLSVLSPPLSAGAKVPPPPPLRNSRVERTLLETSLRLQQEQSHQHQNGTRFQLSDPPKQNPRTAPTHVTPASAAASVDGQLLHLSSASPSPSQSSLHRPSPHTSGKSTPASSAGMATLPPSQLQTVREQMATALKQLKEMEERVKGVPVLEREVAKLQAEKDMLLLALQEKKTLVAQQQATTVVSSTNTTTLDAGTWSQERPILSPKCPRCPKSPCKLGDLRKLTEKFEGKTEKDGARSEKVPEKVVEKKSVAIGDDMLLDAGVFYYSLGTKDASEGTLQMDVCEKGMATEAPAIHEEGVQAVVETEEASVWVMESQLGLSSDAQREIDTLQDNIKFQQESISVLEGRLGQADEVLAVLKAQENERKSKATSDKGVLVKPDSAHAQVGTEASTMSAPASQNVAVSCCPEVVDACVGEDLRAGYYDQSTQTDSVEIPAEAESTPVVLVSTGSQWENLYMEEDIEQKAPVTALKRRQTTIAEYKVNPGKETVSLPKGKGVKEEEMAPSTPTTATTVGSMLKSIMKKKDGSSSSESRSSGKKSLQFVGILNGGYESTSSEEEEDDDEEEGSSSGGSEVGDCSDGSDDEDAAAALEETSDEERSMNMDDTDSDDVALAAGTGATEEIPDKVKEKFELSAKMREACLILKNHLNDDVKTLKSKEVISSTHTVQLEWFRISSAKLAQQPRVSDYLMAFTEVSSALLAHVVNITDGNGNTALHYSVSHSNFTVVGLLLDTGMCCVDKQNKAGYTAIMLAALSAVKEEDDMVVVRKLFSQGNVNAKASQAGQTGLMLAVSHGRQEMVRALLDCGADVNVQDDEGSTALMCASEHGRAEIVSLLLDQPGCDISIVDNDGSNALSIALEASHNDTAVLLYAHMNYAKAQTAGTTKTRSPTSPQKTWPAAE

Target ORF information:

RefSeq Version	XM_021572213.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss KN motif and ankyrin repeat domain-containing protein 2-like (LOC110496290), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021572213.1

ORF Insert Sequence:

ATGACTCAGT CTGTTCAGGT CAACCCAAAG CTGCCTGATC TGGGCGCACC GTTTCTATAC 
TCCAGTCAGG AAGAGGCGGA TCAACAGGGC TCTTATTCGG TTCAGACACC GTATGGCTTC 
CAGCTGGACC TGGACTTCCT CAAGTATGTG GAGGAAATAG AAAGCGGCCA CCACGTCCGA 
CGGGCACCCG TCAACTCCCG CAGGTCATCC CGGGGGGTCA AAGTCTCCCA GCGGAGCCCG 
AGTGTGGGGG GACGCGCCAG CGGCTGGACC TCCACAGAGT CCCTCTCCTC GCCCGCCAGC 
GAAGATGGTC GAGTGCCCCC TCCCCCGCCC CCACGGAATC GCATTGGCTC TGCTCCCAGT 
GAGGGGCAAC TCCTGTCCTC GCTATCTGTC CTCAGCCCTC CTCTCTCTGC CGGCGCCAAA 
GTGCCACCAC CACCTCCACT ACGTAACTCC AGAGTAGAGA GGACCCTCCT GGAGACCAGC 
CTGAGACTGC AGCAGGAGCA GAGCCATCAG CACCAAAATG GCACCCGTTT CCAGCTCTCA 
GACCCACCAA AGCAAAACCC CAGGACTGCC CCTACCCATG TCACCCCAGC TAGTGCAGCA 
GCCAGCGTGG ATGGACAGCT CTTGCACCTG TCCTCTGCCT CTCCCAGCCC ATCTCAGAGT 
AGCCTGCACA GACCCAGTCC CCACACCTCC GGTAAGAGCA CCCCGGCCTC TAGCGCCGGA 
ATGGCTACTC TGCCCCCCAG CCAATTGCAG ACGGTGAGAG AACAGATGGC CACTGCCCTG 
AAGCAACTGA AGGAGATGGA GGAGCGAGTA AAGGGTGTCC CAGTGCTGGA AAGAGAGGTG 
GCCAAGCTAC AGGCTGAGAA AGACATGCTC CTACTGGCAC TGCAGGAGAA GAAGACCTTG 
GTGGCCCAAC AACAGGCTAC AACAGTAGTC AGCAGTACTA ATACCACCAC ATTGGACGCA 
GGCACATGGA GTCAGGAACG TCCTATTTTA TCCCCTAAGT GTCCCAGATG TCCAAAGAGT 
CCCTGTAAAT TAGGGGACCT CAGAAAGCTG ACAGAGAAGT TCGAAGGCAA GACAGAAAAA 
GATGGAGCAC GTTCTGAGAA AGTTCCGGAA AAGGTTGTGG AGAAGAAGTC TGTGGCCATC 
GGGGACGACA TGTTGCTGGA TGCTGGTGTC TTCTACTACA GCCTGGGCAC CAAGGACGCC 
TCAGAGGGCA CGCTGCAGAT GGACGTCTGC GAGAAAGGAA TGGCCACGGA GGCGCCGGCC 
ATCCACGAGG AGGGGGTGCA GGCTGTAGTG GAGACAGAGG AGGCCTCAGT TTGGGTGATG 
GAATCCCAGC TGGGCCTAAG CAGCGATGCC CAGAGGGAGA TCGACACCCT ACAGGACAAC 
ATCAAGTTCC AGCAGGAGTC CATCTCGGTT CTGGAGGGGC GACTTGGCCA GGCTGACGAG 
GTCCTGGCGG TGCTCAAAGC CCAGGAGAAT GAAAGGAAGT CCAAGGCAAC GTCCGACAAG 
GGGGTCCTTG TCAAACCAGA CTCTGCCCAT GCCCAAGTGG GGACTGAAGC CTCTACAATG 
TCCGCACCAG CTTCACAGAA TGTCGCAGTC TCCTGTTGTC CTGAGGTGGT TGACGCATGT 
GTAGGTGAGG ATTTGAGAGC CGGATATTAC GACCAGAGCA CTCAGACTGA CTCTGTGGAG 
ATCCCTGCAG AAGCGGAATC AACCCCAGTA GTACTGGTCA GCACTGGAAG TCAATGGGAG 
AATCTGTACA TGGAGGAGGA CATAGAGCAG AAAGCTCCAG TCACTGCGCT GAAGAGGAGA 
CAGACGACCA TCGCGGAGTA CAAGGTCAAC CCTGGCAAGG AGACGGTCAG TTTACCCAAA 
GGAAAGGGAG TGAAAGAGGA GGAAATGGCG CCCAGCACTC CAACAACTGC AACAACGGTT 
GGAAGTATGT TGAAATCAAT CATGAAGAAG AAGGATGGGA GTAGTTCCAG TGAATCACGC 
AGCAGCGGCA AGAAAAGCTT GCAGTTTGTT GGCATTCTCA ACGGGGGGTA TGAGTCGACA 
TCTAGTGAAG AGGAGGAGGA TGATGATGAG GAAGAGGGGA GTTCCTCAGG TGGCAGTGAA 
GTAGGTGATT GCTCAGACGG TAGTGATGAT GAAGATGCAG CGGCCGCTCT GGAGGAGACC 
TCCGATGAGG AGAGGAGCAT GAATATGGAT GACACCGATA GTGATGATGT GGCCTTAGCA 
GCAGGGACTG GAGCCACTGA GGAGATTCCA GATAAAGTGA AAGAGAAGTT TGAGCTGAGT 
GCAAAAATGC GTGAAGCTTG TCTCATTTTG AAGAACCATC TGAATGATGA TGTCAAAACA 
CTGAAGAGTA AAGAAGTGAT CTCCAGCACT CACACTGTGC AGCTGGAATG GTTCCGCATC 
TCCAGTGCCA AGCTGGCCCA GCAGCCCCGT GTCTCAGACT ACCTGATGGC CTTCACAGAA 
GTCTCCTCTG CCTTGCTGGC CCACGTCGTC AACATTACCG ATGGCAATGG CAACACAGCC 
TTGCACTACA GCGTCTCCCA CTCCAACTTC ACCGTGGTGG GGCTACTGCT GGACACAGGC 
ATGTGTTGTG TAGATAAGCA GAACAAGGCA GGCTACACTG CGATCATGCT GGCGGCCCTC 
TCAGCTGTGA AAGAGGAGGA TGACATGGTG GTGGTCAGGA AGCTCTTCAG TCAGGGCAAC 
GTCAACGCCA AGGCCAGCCA GGCTGGCCAG ACGGGGCTGA TGCTAGCTGT TAGCCATGGA 
CGGCAGGAGA TGGTGCGGGC ATTGCTGGAC TGTGGCGCTG ACGTGAACGT GCAGGATGAC 
GAGGGCTCCA CGGCGCTCAT GTGCGCCAGC GAGCACGGCC GCGCCGAGAT TGTCTCGCTG 
CTCCTGGACC AGCCGGGCTG TGACATCTCC ATTGTGGACA ATGACGGCAG TAATGCTCTG 
TCCATCGCGC TGGAGGCCTC TCACAATGAC ACAGCTGTGC TGCTTTACGC CCACATGAAC 
TACGCCAAAG CCCAGACAGC TGGGACAACC AAGACTCGAA GCCCCACTAG TCCTCAAAAG 
ACATGGCCTG CTGCGGAGTA A