LOC100210444 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100210444
Entrez Gene ID	100210444
Full Name	glycine--tRNA ligase-like
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_002157098.3	XP_002157134.2	glycine--tRNA ligase-like

Pathways from BioSystems
Gene Ontology

The following LOC100210444 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100210444 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc05720	XM_002157098.3 Latest version!	Hydra vulgaris glycine--tRNA ligase-like (LOC100210444), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100210444 ( XM_002157098.3 ) cDNA ORF clone, Hydra vulgaris -> XP_002157134.2 Hydra vulgaris glycine--tRNA ligase-like (LOC100210444), mRNA.

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CloneID	OHc05720
Clone ID Related Accession (Same CDS sequence)	XM_002157098.3
Accession Version	XM_002157098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Hydra vulgaris
Product	glycine--tRNA ligase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004168118.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_002157098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTAT TGAAATTGTT AGTTTTAAAC ACAAAATTTA TTTTCAAAAA AAAGTTTAAA 
TTCTACAATA GCAGTACACT TCATACCAGT TATCAATCAT TGTTTATTGG TTTAGAAAAA 
TTCTTATCTA CATTTCCTCG TTCATACGAT AAATTCATGT CAGATCTAGA GCTTGAGATA 
ACTCAGTGTA GGGCGTCAGT AAAGGAACAG GGTGATTTGG TTAAAAAACT CAAAAGTGAA 
GGCGCTCAAA CTATTGATAT TGATTTGGCA GTAAAGGAAC TTAAAGCACG CAAAAGAGTA 
CTAGAACAAA AAGAATTGCT CCTTGATCCT CCTGATACTT TTGATAGAGC AAGATGTGAA 
GATTTATTGA AAAGGCGATT TTTTTATGCT CCTGCTTTTG AGATATATGG AGGTGTTGCT 
GGTCTATATG ACTTTGGACC TATGGGGTGT GCAATGGAGA ACAACTTGTT AGCAATATGG 
CGATCGCATT TTGTTCTGGA AGAACAGTTT TTAGAGGTTC GTTGCACACA ATTAACACCA 
ATTCAAGTTC TTAAGGCATC AGGGCACGTT GATAGATTTA CTGATGTTAT GGTGCGTGAT 
ATTAAAACGG GTGACTGTTA CCGGGCTGAT CATCTTCTTG AAGGCCATCT TGAAAATTTA 
TTGAGCAACA AGAAGCTGTC TGAAGACAAA AGAAAGGAAT ACACGTTAGT TCTAAGTCAG 
ATTGATAACT TTAAAAAAGA TGATCTTTGG AACCTTATTC AAAAGTATGA ATGCAAAGCT 
CCTGGAACAA ACAATGATTT GCATGATCCA CAAGATTTTA ATCTCATGTT TGGTACGTCT 
ATCGGACCAG GTGGAAATCT TGCTGGTTTT ATGAGACCTG AGACAGCTCA AGGAATTTTC 
CTTAATTTTA AAAGGCTTTT GGAATATAAT AATGGAAGGT TACCATTTGG AGGAGCGCAA 
ATTGGTTGTG GATTCAGAAA TGAAATAGCT CCACGAGCAG GTTTACTTCG AGTCAGAGAG 
TTTATTATGG CTGAAATAGA ATATTTTGTT GATCCAGAAG AAAAAGAAAT AACTAAATTT 
AAAGATGTGC AAGACACAGT ATGCGTGTTG TACCCTCGTG ATGTCCAAAT GGCTGGCGAA 
CCTGCACTAA AAATGACTTT AAAGGAAGCA CTTGAAAAAG GAATCATTTT TAACACAGCA 
ATGGGTTACT TTCTTGCTCG CATTCAAAGT TTTATGTTGA AAATAGGTGT TGATCCTACC 
AAAATGCGGT TTAGGCAGCA TATGGCCAAT GAAATGGCAC ACTATGCTTG TGATTGCTGG 
GATTGTGAAC TTAAAACATC ATATGGTTGG GTTGAATGTG TTGGCTGTGC AGACCGCGCC 
TGTTTTGATC TTCAACAGCA TACAAACGCG AGTGGCGAAA AACTTGTTGC TATGGTTGAT 
TTAGCTACAC CTGTTTCTGT TGATGTAGTT GAAGTATGTC TTGAAAAATC TGCTGTAGGA 
AAATCTTTTA AAAATGAAGC TAAAGCAGTC ACTGAGTATG TTGCTAAAAT GACTGTTGAT 
GAAATTGAAA AGTTGGAGTT ATCCATTAAA GAAAACGGTT TGTTTTCGAT TAATGTGAAT 
GACAAAGAGT ATGTTTTAAA AAAGGAAATG GTTAAAGAAG TTCGTAGATT TAAGAAAGAT 
ATTCATGTCC GAGAAGTTTC GCCACATGTT GTAGAGCCCT CGTTTGGGAT TGGTAGAATT 
ATGTATGCTG TTTTAGAGCA AAATTTTCGT GTAAGAGAAG GTGACGAACA AAGAACTTGG 
TTGTCACTTC CTCCGTTACT AGCCCCTGTA AAATGTTCTG TACTTCCTCT GAGCAAGAAT 
GAAGCATTTG ATCCATTTGT CAAAAAAATA TCGGCCATGC TGACTGAATT AGATGTCTCT 
CACAAAGTTG ATGATTCAAG TATATCAATA GGAAGACGTT ACGCTCGTAC TGATGAAATA 
GCTATACCAT ACGGAGTGAC AGTAGATTTT GATACAATAA ATAAAGAACC AAACACTGTT 
ACACTCAGAG AAAGAAATAC TACAAAACAG ATTCGTGTTG AAGTAAACTT TGTAGGTGAG 
TTAGTAAGAG ATTTGGTGAA AGGTAAACGA GTTTGGGACG ACGTTGTTGA AGAGTTTGGT 
CTCTTTGAAG GTCAACAAAC TATCACAAAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002157134.2
CDS	108..2300
Translation	MLLLKLLVLNTKFIFKKKFKFYNSSTLHTSYQSLFIGLEKFLSTFPRSYDKFMSDLELEITQCRASVKEQGDLVKKLKSEGAQTIDIDLAVKELKARKRVLEQKELLLDPPDTFDRARCEDLLKRRFFYAPAFEIYGGVAGLYDFGPMGCAMENNLLAIWRSHFVLEEQFLEVRCTQLTPIQVLKASGHVDRFTDVMVRDIKTGDCYRADHLLEGHLENLLSNKKLSEDKRKEYTLVLSQIDNFKKDDLWNLIQKYECKAPGTNNDLHDPQDFNLMFGTSIGPGGNLAGFMRPETAQGIFLNFKRLLEYNNGRLPFGGAQIGCGFRNEIAPRAGLLRVREFIMAEIEYFVDPEEKEITKFKDVQDTVCVLYPRDVQMAGEPALKMTLKEALEKGIIFNTAMGYFLARIQSFMLKIGVDPTKMRFRQHMANEMAHYACDCWDCELKTSYGWVECVGCADRACFDLQQHTNASGEKLVAMVDLATPVSVDVVEVCLEKSAVGKSFKNEAKAVTEYVAKMTVDEIEKLELSIKENGLFSINVNDKEYVLKKEMVKEVRRFKKDIHVREVSPHVVEPSFGIGRIMYAVLEQNFRVREGDEQRTWLSLPPLLAPVKCSVLPLSKNEAFDPFVKKISAMLTELDVSHKVDDSSISIGRRYARTDEIAIPYGVTVDFDTINKEPNTVTLRERNTTKQIRVEVNFVGELVRDLVKGKRVWDDVVEEFGLFEGQQTITK

Target ORF information:

RefSeq Version	XM_002157098.3
Organism	Hydra vulgaris
Definition	Hydra vulgaris glycine--tRNA ligase-like (LOC100210444), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002157098.3

ORF Insert Sequence:

ATGTTGTTAT TGAAATTGTT AGTTTTAAAC ACAAAATTTA TTTTCAAAAA AAAGTTTAAA 
TTCTACAATA GCAGTACACT TCATACCAGT TATCAATCAT TGTTTATTGG TTTAGAAAAA 
TTCTTATCTA CATTTCCTCG TTCATACGAT AAATTCATGT CAGATCTAGA GCTTGAGATA 
ACTCAGTGTA GGGCGTCAGT AAAGGAACAG GGTGATTTGG TTAAAAAACT CAAAAGTGAA 
GGCGCTCAAA CTATTGATAT TGATTTGGCA GTAAAGGAAC TTAAAGCACG CAAAAGAGTA 
CTAGAACAAA AAGAATTGCT CCTTGATCCT CCTGATACTT TTGATAGAGC AAGATGTGAA 
GATTTATTGA AAAGGCGATT TTTTTATGCT CCTGCTTTTG AGATATATGG AGGTGTTGCT 
GGTCTATATG ACTTTGGACC TATGGGGTGT GCAATGGAGA ACAACTTGTT AGCAATATGG 
CGATCGCATT TTGTTCTGGA AGAACAGTTT TTAGAGGTTC GTTGCACACA ATTAACACCA 
ATTCAAGTTC TTAAGGCATC AGGGCACGTT GATAGATTTA CTGATGTTAT GGTGCGTGAT 
ATTAAAACGG GTGACTGTTA CCGGGCTGAT CATCTTCTTG AAGGCCATCT TGAAAATTTA 
TTGAGCAACA AGAAGCTGTC TGAAGACAAA AGAAAGGAAT ACACGTTAGT TCTAAGTCAG 
ATTGATAACT TTAAAAAAGA TGATCTTTGG AACCTTATTC AAAAGTATGA ATGCAAAGCT 
CCTGGAACAA ACAATGATTT GCATGATCCA CAAGATTTTA ATCTCATGTT TGGTACGTCT 
ATCGGACCAG GTGGAAATCT TGCTGGTTTT ATGAGACCTG AGACAGCTCA AGGAATTTTC 
CTTAATTTTA AAAGGCTTTT GGAATATAAT AATGGAAGGT TACCATTTGG AGGAGCGCAA 
ATTGGTTGTG GATTCAGAAA TGAAATAGCT CCACGAGCAG GTTTACTTCG AGTCAGAGAG 
TTTATTATGG CTGAAATAGA ATATTTTGTT GATCCAGAAG AAAAAGAAAT AACTAAATTT 
AAAGATGTGC AAGACACAGT ATGCGTGTTG TACCCTCGTG ATGTCCAAAT GGCTGGCGAA 
CCTGCACTAA AAATGACTTT AAAGGAAGCA CTTGAAAAAG GAATCATTTT TAACACAGCA 
ATGGGTTACT TTCTTGCTCG CATTCAAAGT TTTATGTTGA AAATAGGTGT TGATCCTACC 
AAAATGCGGT TTAGGCAGCA TATGGCCAAT GAAATGGCAC ACTATGCTTG TGATTGCTGG 
GATTGTGAAC TTAAAACATC ATATGGTTGG GTTGAATGTG TTGGCTGTGC AGACCGCGCC 
TGTTTTGATC TTCAACAGCA TACAAACGCG AGTGGCGAAA AACTTGTTGC TATGGTTGAT 
TTAGCTACAC CTGTTTCTGT TGATGTAGTT GAAGTATGTC TTGAAAAATC TGCTGTAGGA 
AAATCTTTTA AAAATGAAGC TAAAGCAGTC ACTGAGTATG TTGCTAAAAT GACTGTTGAT 
GAAATTGAAA AGTTGGAGTT ATCCATTAAA GAAAACGGTT TGTTTTCGAT TAATGTGAAT 
GACAAAGAGT ATGTTTTAAA AAAGGAAATG GTTAAAGAAG TTCGTAGATT TAAGAAAGAT 
ATTCATGTCC GAGAAGTTTC GCCACATGTT GTAGAGCCCT CGTTTGGGAT TGGTAGAATT 
ATGTATGCTG TTTTAGAGCA AAATTTTCGT GTAAGAGAAG GTGACGAACA AAGAACTTGG 
TTGTCACTTC CTCCGTTACT AGCCCCTGTA AAATGTTCTG TACTTCCTCT GAGCAAGAAT 
GAAGCATTTG ATCCATTTGT CAAAAAAATA TCGGCCATGC TGACTGAATT AGATGTCTCT 
CACAAAGTTG ATGATTCAAG TATATCAATA GGAAGACGTT ACGCTCGTAC TGATGAAATA 
GCTATACCAT ACGGAGTGAC AGTAGATTTT GATACAATAA ATAAAGAACC AAACACTGTT 
ACACTCAGAG AAAGAAATAC TACAAAACAG ATTCGTGTTG AAGTAAACTT TGTAGGTGAG 
TTAGTAAGAG ATTTGGTGAA AGGTAAACGA GTTTGGGACG ACGTTGTTGA AGAGTTTGGT 
CTCTTTGAAG GTCAACAAAC TATCACAAAG TAG