MAP4K4 gene cDNA ORF clone, Chlorocebus sabaeus(green monkey)

mRNA	Protein	Name
XM_008008217.1	XP_008006408.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
XM_008008218.1	XP_008006409.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
XM_008008221.1	XP_008006412.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
XM_008008234.1	XP_008006425.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
XM_008008212.1	XP_008006403.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
XM_008008213.1	XP_008006404.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
XM_008008214.1	XP_008006405.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
XM_008008224.1	XP_008006415.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
XM_008008226.1	XP_008006417.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
XM_008008227.1	XP_008006418.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
XM_008008228.1	XP_008006419.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
XM_008008231.1	XP_008006422.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
XM_008008211.1	XP_008006402.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
XM_008008215.1	XP_008006406.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
XM_008008223.1	XP_008006414.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
XM_008008230.1	XP_008006421.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
XM_008008233.1	XP_008006424.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
XM_008008222.1	XP_008006413.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
XM_008008216.1	XP_008006407.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
XM_008008219.1	XP_008006410.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
XM_008008220.1	XP_008006411.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
XM_008008229.1	XP_008006420.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
XM_008008232.1	XP_008006423.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X21

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh101914	XM_008008211.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101915	XM_008008212.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101916	XM_008008213.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101917	XM_008008214.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101918	XM_008008215.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101919	XM_008008216.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101920	XM_008008217.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101921	XM_008008218.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCh101922	XM_008008219.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101923	XM_008008220.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101924	XM_008008221.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101925	XM_008008222.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101926	XM_008008223.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101927	XM_008008224.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101928	XM_008008226.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101929	XM_008008227.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101930	XM_008008228.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101931	XM_008008229.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101932	XM_008008230.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101933	XM_008008231.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101934	XM_008008232.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101935	XM_008008233.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCh101936	XM_008008234.1 Latest version!	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

MAP4K4 ( XM_008008211.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006402.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh101914
Clone ID Related Accession (Same CDS sequence)	XM_008008211.1
Accession Version	XM_008008211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC 
ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC 
TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGAACAACA 
GTGACTTCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CGGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAT 
ACCCCAGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AAATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT GATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAAAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC CGGATCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA 
AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TTTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC GTCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006402.1
CDS	178..4359
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008211.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008211.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC 
ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC 
TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGAACAACA 
GTGACTTCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CGGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAT 
ACCCCAGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AAATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT GATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAAAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC CGGATCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA 
AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TTTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC GTCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008212.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006403.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCh101915
Clone ID Related Accession (Same CDS sequence)	XM_008008212.1
Accession Version	XM_008008212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGTAT TGAGCCCAGG 
CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGACCTG GCAGTGGCAG CTCCTCAGGG 
TCCAGCAACT CAGGATCCCA GCCCGGGTCT CACCCTGGGT CTCAGAGTGG CTCCGGGGAA 
CGCTTCAGAG TGAGATCGTC ATCCAAGTCT GAAGGCTCTC CATCTCAGCG CCTGGACAAC 
GCAGTGAAAA AACCTGAAGA TAAAAAAGAA GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA 
GTGGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCGGTGG AAGATGTACG GCCCCCTCAC 
AAAGTGACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC 
GATGTGGAGC AGGAAGGGGC CGACGAGTCC ACCTCGGGAC CGGAGGACAC CAGAGCAGCG 
TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT 
GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG 
AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG AACAACAGTG 
ACTTCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCGG AAGCCATAAG GCAAGATCCT 
ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGATACC 
CCAGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAAA TTCTGTGTGC TGCCTTATGG 
GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTCT ATCCTCTGAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA 
ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA 
TTTCTGGTGA TTGCTTTGAA AAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT 
CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGATG TGGATTCCGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG 
AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTTT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACGTC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006403.1
CDS	178..4356
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008212.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008212.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGTAT TGAGCCCAGG 
CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGACCTG GCAGTGGCAG CTCCTCAGGG 
TCCAGCAACT CAGGATCCCA GCCCGGGTCT CACCCTGGGT CTCAGAGTGG CTCCGGGGAA 
CGCTTCAGAG TGAGATCGTC ATCCAAGTCT GAAGGCTCTC CATCTCAGCG CCTGGACAAC 
GCAGTGAAAA AACCTGAAGA TAAAAAAGAA GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA 
GTGGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCGGTGG AAGATGTACG GCCCCCTCAC 
AAAGTGACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC 
GATGTGGAGC AGGAAGGGGC CGACGAGTCC ACCTCGGGAC CGGAGGACAC CAGAGCAGCG 
TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT 
GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG 
AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGTGG AACAACAGTG 
ACTTCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCGG AAGCCATAAG GCAAGATCCT 
ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGATACC 
CCAGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAAA TTCTGTGTGC TGCCTTATGG 
GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTCT ATCCTCTGAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA 
ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA 
TTTCTGGTGA TTGCTTTGAA AAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT 
CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGATG TGGATTCCGG ATCAGTCTAT GACATTTATC TACCAACACA CGTAAGAAAG 
AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTTT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACGTC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008213.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006404.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCh101916
Clone ID Related Accession (Same CDS sequence)	XM_008008213.1
Accession Version	XM_008008213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGTGGC CCCACCTGGC ATCTGTCAAG AACAATGTTT CCCCTGTCTC GCGATCCCAT 
TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGTTC TCAGGACCCA 
TGTCCACCTT CCCGCAGTGA GGTGCTCGGT CAGAGCGCTG ACTCTAAGTC AGAGACGCCT 
GACCCTACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCT 
GTGAGAACAA CGTCTCGCTC CCCTGTTCTG TCCCGTCGAG ATTCCCCACT GCAGGGCAGT 
GGGCAGCAGA ATAGCCAGGC AGGACAGAGA AACTCCACCA GCAGTATTGA GCCCAGGCTT 
CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC AGACCTGGCA GTGGCAGCTC CTCAGGGTCC 
AGCAACTCAG GATCCCAGCC CGGGTCTCAC CCTGGGTCTC AGAGTGGCTC CGGGGAACGC 
TTCAGAGTGA GATCGTCATC CAAGTCTGAA GGCTCTCCAT CTCAGCGCCT GGACAACGCA 
GTGAAAAAAC CTGAAGATAA AAAAGAAGTT TTCAGACCCC TCAAGCCTGC TGGCGAAGTG 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCGGTGGAAG ATGTACGGCC CCCTCACAAA 
GTGACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCCGA CGAGTCCACC TCGGGACCGG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGAGT 
ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC 
CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC 
CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGAAC AACAGTGACT 
TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCGGAAG CCATAAGGCA AGATCCTACC 
CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGATACCCCA 
GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAAATTC TGTGTGCTGC CTTATGGGGA 
GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTCTATC CTCTGATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC 
GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT 
CTGGTGATTG CTTTGAAAAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC 
AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGATGTGG ATTCCGGATC AGTCTATGAC ATTTATCTAC CAACACACGT AAGAAAGAAC 
CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA 
GATGGAATGG AGCTTTTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA 
AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACGTCAGT AGCATATATT 
CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT 
GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA 
CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC 
ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006404.1
CDS	178..4353
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008213.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008213.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGTGGC CCCACCTGGC ATCTGTCAAG AACAATGTTT CCCCTGTCTC GCGATCCCAT 
TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGTTC TCAGGACCCA 
TGTCCACCTT CCCGCAGTGA GGTGCTCGGT CAGAGCGCTG ACTCTAAGTC AGAGACGCCT 
GACCCTACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCT 
GTGAGAACAA CGTCTCGCTC CCCTGTTCTG TCCCGTCGAG ATTCCCCACT GCAGGGCAGT 
GGGCAGCAGA ATAGCCAGGC AGGACAGAGA AACTCCACCA GCAGTATTGA GCCCAGGCTT 
CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC AGACCTGGCA GTGGCAGCTC CTCAGGGTCC 
AGCAACTCAG GATCCCAGCC CGGGTCTCAC CCTGGGTCTC AGAGTGGCTC CGGGGAACGC 
TTCAGAGTGA GATCGTCATC CAAGTCTGAA GGCTCTCCAT CTCAGCGCCT GGACAACGCA 
GTGAAAAAAC CTGAAGATAA AAAAGAAGTT TTCAGACCCC TCAAGCCTGC TGGCGAAGTG 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCGGTGGAAG ATGTACGGCC CCCTCACAAA 
GTGACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCCGA CGAGTCCACC TCGGGACCGG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGAGT 
ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC 
CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC 
CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGAAC AACAGTGACT 
TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCGGAAG CCATAAGGCA AGATCCTACC 
CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGATACCCCA 
GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAAATTC TGTGTGCTGC CTTATGGGGA 
GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTCTATC CTCTGATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC 
GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT 
CTGGTGATTG CTTTGAAAAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC 
AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGATGTGG ATTCCGGATC AGTCTATGAC ATTTATCTAC CAACACACGT AAGAAAGAAC 
CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA 
GATGGAATGG AGCTTTTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA 
AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACGTCAGT AGCATATATT 
CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT 
GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA 
CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC 
ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008214.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006405.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

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CloneID	OCh101917
Clone ID Related Accession (Same CDS sequence)	XM_008008214.1
Accession Version	XM_008008214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCG GTGGAAGATG TACGGCCCCC TCACAAAGTG 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCCGACGA GTCCACCTCG GGACCGGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGAACAAC AGTGACTTCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCGGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA TACCCCAGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAAATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TGATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAAAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGATCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA 
CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTTTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CGTCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006405.1
CDS	178..4350
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008214.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008214.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCG GTGGAAGATG TACGGCCCCC TCACAAAGTG 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCCGACGA GTCCACCTCG GGACCGGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGAACAAC AGTGACTTCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCGGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA TACCCCAGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAAATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TGATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAAAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGATCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA 
CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTTTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CGTCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008215.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006406.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCh101918
Clone ID Related Accession (Same CDS sequence)	XM_008008215.1
Accession Version	XM_008008215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC 
ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC 
TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGAACAACA 
GTGACTTCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CGGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAT 
ACCCCAGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AAATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT GATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAAAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC CGGATCAGTC TATGACATTT ATCTACCAAC ACACATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTTTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACGTCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006406.1
CDS	178..4335
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008215.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008215.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCCGGTCGC 
ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCC 
TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG 
CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGAACAACA 
GTGACTTCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CGGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAT 
ACCCCAGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AAATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT GATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAAAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC CGGATCAGTC TATGACATTT ATCTACCAAC ACACATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTTTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACGTCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008216.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006407.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCh101919
Clone ID Related Accession (Same CDS sequence)	XM_008008216.1
Accession Version	XM_008008216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGCCCCACCT GGCATCTGTC 
AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
TTTGCACACC ACCATCTTCG TTCTCAGGAC CCATGTCCAC CTTCCCGCAG TGAGGTGCTC 
GGTCAGAGCG CTGACTCTAA GTCAGAGACG CCTGACCCTA CCCAAAAGGC TTGGTCTAGA 
TCAGACAGTG ACGAGGTGCC TCCAAGGGTT CCTGTGAGAA CAACGTCTCG CTCCCCTGTT 
CTGTCCCGTC GAGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAATAGCCA GGCAGGACAG 
AGAAACTCCA CCAGCAGTAT TGAGCCCAGG CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG 
CCCAGACCTG GCAGTGGCAG CTCCTCAGGG TCCAGCAACT CAGGATCCCA GCCCGGGTCT 
CACCCTGGGT CTCAGAGTGG CTCCGGGGAA CGCTTCAGAG TGAGATCGTC ATCCAAGTCT 
GAAGGCTCTC CATCTCAGCG CCTGGACAAC GCAGTGAAAA AACCTGAAGA TAAAAAAGAA 
GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA GTGGATCTGA CCGCACTGGC CAAAGAGCTT 
CGAGCGGTGG AAGATGTACG GCCCCCTCAC AAAGTGACGG ACTACTCCTC ATCCAGTGAG 
GAGTCGGGGA CGACGGATGA GGAGGACGAC GATGTGGAGC AGGAAGGGGC CGACGAGTCC 
ACCTCGGGAC CGGAGGACAC CAGAGCAGCG TCATCTCTGA ATTTGAGCAA TGGTGAAACG 
GAATCTGTGA AAACCATGAT TGTCCATGAT GATGTAGAAA GTGAGCCGGC CATGACCCCA 
TCCAAGGAGG GCACTCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG TGCCAGCCAT 
CATGAGAGCA ATGGCTTTGC CGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
CATTCCTCCT CCACTTCCTC CACCTCCTCC TCCCCATCCT CCAGCCAGCC GACACCCACC 
ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTGCTAATG AGACTCAGTC CGCTAGTAGC 
ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
CAGATTTCTC CATCTAGTGG AACAACAGTG ACTTCTGTGG TGGGATTTTC CTGTGATGGG 
ATGAGACCGG AAGCCATAAG GCAAGATCCT ACCCGGAAAG GCTCAGTGGT CAATGTGAAT 
CCTACCAACA CTAGGCCACA GAGTGATACC CCAGAGATTC GTAAATACAA GAAGAGGTTT 
AACTCTGAAA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT 
GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTCT ATCCTCTGAT CAACCGAAGA 
CGATTTCAAC AAATGGACGT ACTTGAGGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA 
AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT 
CCAGAAGTTG AGAAGAAGCA GGGATGGACA ACCGTAGGGG ATTTGGAAGG ATGTGTACAT 
TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTGA TTGCTTTGAA AAGTTCTGTG 
GAAGTCTATG CGTGGGCACC AAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA 
GAATTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGTTGAAA 
GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCCGG ATCAGTCTAT 
GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA GTGTAGCATC 
AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTTT GGTGTGCTAT 
GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG 
TGGGGAGAGA TGCCTACGTC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC 
AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT 
GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006407.1
CDS	178..4266
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008216.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008216.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGCCCCACCT GGCATCTGTC 
AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
TTTGCACACC ACCATCTTCG TTCTCAGGAC CCATGTCCAC CTTCCCGCAG TGAGGTGCTC 
GGTCAGAGCG CTGACTCTAA GTCAGAGACG CCTGACCCTA CCCAAAAGGC TTGGTCTAGA 
TCAGACAGTG ACGAGGTGCC TCCAAGGGTT CCTGTGAGAA CAACGTCTCG CTCCCCTGTT 
CTGTCCCGTC GAGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAATAGCCA GGCAGGACAG 
AGAAACTCCA CCAGCAGTAT TGAGCCCAGG CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG 
CCCAGACCTG GCAGTGGCAG CTCCTCAGGG TCCAGCAACT CAGGATCCCA GCCCGGGTCT 
CACCCTGGGT CTCAGAGTGG CTCCGGGGAA CGCTTCAGAG TGAGATCGTC ATCCAAGTCT 
GAAGGCTCTC CATCTCAGCG CCTGGACAAC GCAGTGAAAA AACCTGAAGA TAAAAAAGAA 
GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA GTGGATCTGA CCGCACTGGC CAAAGAGCTT 
CGAGCGGTGG AAGATGTACG GCCCCCTCAC AAAGTGACGG ACTACTCCTC ATCCAGTGAG 
GAGTCGGGGA CGACGGATGA GGAGGACGAC GATGTGGAGC AGGAAGGGGC CGACGAGTCC 
ACCTCGGGAC CGGAGGACAC CAGAGCAGCG TCATCTCTGA ATTTGAGCAA TGGTGAAACG 
GAATCTGTGA AAACCATGAT TGTCCATGAT GATGTAGAAA GTGAGCCGGC CATGACCCCA 
TCCAAGGAGG GCACTCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG TGCCAGCCAT 
CATGAGAGCA ATGGCTTTGC CGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
CATTCCTCCT CCACTTCCTC CACCTCCTCC TCCCCATCCT CCAGCCAGCC GACACCCACC 
ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTGCTAATG AGACTCAGTC CGCTAGTAGC 
ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
CAGATTTCTC CATCTAGTGG AACAACAGTG ACTTCTGTGG TGGGATTTTC CTGTGATGGG 
ATGAGACCGG AAGCCATAAG GCAAGATCCT ACCCGGAAAG GCTCAGTGGT CAATGTGAAT 
CCTACCAACA CTAGGCCACA GAGTGATACC CCAGAGATTC GTAAATACAA GAAGAGGTTT 
AACTCTGAAA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT 
GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTCT ATCCTCTGAT CAACCGAAGA 
CGATTTCAAC AAATGGACGT ACTTGAGGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA 
AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT 
CCAGAAGTTG AGAAGAAGCA GGGATGGACA ACCGTAGGGG ATTTGGAAGG ATGTGTACAT 
TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTGA TTGCTTTGAA AAGTTCTGTG 
GAAGTCTATG CGTGGGCACC AAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA 
GAATTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGTTGAAA 
GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCCGG ATCAGTCTAT 
GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA GTGTAGCATC 
AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTTT GGTGTGCTAT 
GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG 
TGGGGAGAGA TGCCTACGTC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC 
AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT 
GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008217.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006408.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

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CloneID	OCh101920
Clone ID Related Accession (Same CDS sequence)	XM_008008217.1
Accession Version	XM_008008217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTACAG TGGCCCCACC TGGCATCTGT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC 
CACCATCTTC GTTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT CGGTCAGAGC 
GCTGACTCTA AGTCAGAGAC GCCTGACCCT ACCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACAACGTCTC GCTCCCCTGT TCTGTCCCGT 
CGAGATTCCC CACTGCAGGG CAGTGGGCAG CAGAATAGCC AGGCAGGACA GAGAAACTCC 
ACCAGCAGTA TTGAGCCCAG GCTTCTGTGG GAGAGAGTGG AGAAGCTGGT GCCCAGACCT 
GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC TCAGGATCCC AGCCCGGGTC TCACCCTGGG 
TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA GTGAGATCGT CATCCAAGTC TGAAGGCTCT 
CCATCTCAGC GCCTGGACAA CGCAGTGAAA AAACCTGAAG ATAAAAAAGA AGTTTTCAGA 
CCCCTCAAGC CTGCTGGCGA AGTGGATCTG ACCGCACTGG CCAAAGAGCT TCGAGCGGTG 
GAAGATGTAC GGCCCCCTCA CAAAGTGACG GACTACTCCT CATCCAGTGA GGAGTCGGGG 
ACGACGGATG AGGAGGACGA CGATGTGGAG CAGGAAGGGG CCGACGAGTC CACCTCGGGA 
CCGGAGGACA CCAGAGCAGC GTCATCTCTG AATTTGAGCA ATGGTGAAAC GGAATCTGTG 
AAAACCATGA TTGTCCATGA TGATGTAGAA AGTGAGCCGG CCATGACCCC ATCCAAGGAG 
GGCACTCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CCGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCCTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
CCATCTAGTG GAACAACAGT GACTTCTGTG GTGGGATTTT CCTGTGATGG GATGAGACCG 
GAAGCCATAA GGCAAGATCC TACCCGGAAA GGCTCAGTGG TCAATGTGAA TCCTACCAAC 
ACTAGGCCAC AGAGTGATAC CCCAGAGATT CGTAAATACA AGAAGAGGTT TAACTCTGAA 
ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG 
CTGCTGGACA GAAGTGGCCA AGGGAAGGTC TATCCTCTGA TCAACCGAAG ACGATTTCAA 
CAAATGGACG TACTTGAGGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT 
GAGAAGAAGC AGGGATGGAC AACCGTAGGG GATTTGGAAG GATGTGTACA TTATAAAGTT 
GTAAAATATG AAAGAATCAA ATTTCTGGTG ATTGCTTTGA AAAGTTCTGT GGAAGTCTAT 
GCGTGGGCAC CAAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAATTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GATCAGTCTA TGACATTTAT 
CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTT TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACGT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006408.1
CDS	178..4197
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008217.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008217.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTACAG TGGCCCCACC TGGCATCTGT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCTCCCAA ATTTGCACAC 
CACCATCTTC GTTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGTGCT CGGTCAGAGC 
GCTGACTCTA AGTCAGAGAC GCCTGACCCT ACCCAAAAGG CTTGGTCTAG ATCAGACAGT 
GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACAACGTCTC GCTCCCCTGT TCTGTCCCGT 
CGAGATTCCC CACTGCAGGG CAGTGGGCAG CAGAATAGCC AGGCAGGACA GAGAAACTCC 
ACCAGCAGTA TTGAGCCCAG GCTTCTGTGG GAGAGAGTGG AGAAGCTGGT GCCCAGACCT 
GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC TCAGGATCCC AGCCCGGGTC TCACCCTGGG 
TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA GTGAGATCGT CATCCAAGTC TGAAGGCTCT 
CCATCTCAGC GCCTGGACAA CGCAGTGAAA AAACCTGAAG ATAAAAAAGA AGTTTTCAGA 
CCCCTCAAGC CTGCTGGCGA AGTGGATCTG ACCGCACTGG CCAAAGAGCT TCGAGCGGTG 
GAAGATGTAC GGCCCCCTCA CAAAGTGACG GACTACTCCT CATCCAGTGA GGAGTCGGGG 
ACGACGGATG AGGAGGACGA CGATGTGGAG CAGGAAGGGG CCGACGAGTC CACCTCGGGA 
CCGGAGGACA CCAGAGCAGC GTCATCTCTG AATTTGAGCA ATGGTGAAAC GGAATCTGTG 
AAAACCATGA TTGTCCATGA TGATGTAGAA AGTGAGCCGG CCATGACCCC ATCCAAGGAG 
GGCACTCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC 
AATGGCTTTG CCGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
TCCACTTCCT CCACCTCCTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
CCATCTAGTG GAACAACAGT GACTTCTGTG GTGGGATTTT CCTGTGATGG GATGAGACCG 
GAAGCCATAA GGCAAGATCC TACCCGGAAA GGCTCAGTGG TCAATGTGAA TCCTACCAAC 
ACTAGGCCAC AGAGTGATAC CCCAGAGATT CGTAAATACA AGAAGAGGTT TAACTCTGAA 
ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG 
CTGCTGGACA GAAGTGGCCA AGGGAAGGTC TATCCTCTGA TCAACCGAAG ACGATTTCAA 
CAAATGGACG TACTTGAGGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT 
GAGAAGAAGC AGGGATGGAC AACCGTAGGG GATTTGGAAG GATGTGTACA TTATAAAGTT 
GTAAAATATG AAAGAATCAA ATTTCTGGTG ATTGCTTTGA AAAGTTCTGT GGAAGTCTAT 
GCGTGGGCAC CAAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAATTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GATCAGTCTA TGACATTTAT 
CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTT TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACGT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008218.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006409.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCh101921
Clone ID Related Accession (Same CDS sequence)	XM_008008218.1
Accession Version	XM_008008218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGTGGCCC CACCTGGCAT CTGTCAAGAA CAATGTTTCC 
CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
CTTCGTTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG TGCTCGGTCA GAGCGCTGAC 
TCTAAGTCAG AGACGCCTGA CCCTACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
GTGCCTCCAA GGGTTCCTGT GAGAACAACG TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT 
TCCCCACTGC AGGGCAGTGG GCAGCAGAAT AGCCAGGCAG GACAGAGAAA CTCCACCAGC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCGTCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG ACAACGCAGT GAAAAAACCT GAAGATAAAA AAGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC GGTGGAAGAT 
GTACGGCCCC CTCACAAAGT GACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCCGACG AGTCCACCTC GGGACCGGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC 
TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGTGGAACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCGGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ATACCCCAGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAAATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTGATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAAAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCCGGATCAG TCTATGACAT TTATCTACCA 
ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC 
ATCATCCTCC CCAATACAGA TGGAATGGAG CTTTTGGTGT GCTATGAAGA TGAGGGGGTT 
TATGTAAACA CATATGGAAG GATCACCAAG GATGTAGTTC TACAGTGGGG AGAGATGCCT 
ACGTCAGTAG CATATATTCG ATCCAATCAG ACAATGGGCT GGGGAGAGAA GGCCATAGAG 
ATCCGATCTG TGGAAACTGG TCACTTGGAT GGTGTGTTCA TGCACAAAAG GGCTCAAAGA 
CTAAAATTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC 
AGCAGTCAGG TTTATTTCAT GACCTTAGGC AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006409.1
CDS	178..4191
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008218.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008218.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGTGGCCC CACCTGGCAT CTGTCAAGAA CAATGTTTCC 
CCTGTCTCGC GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
CTTCGTTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG TGCTCGGTCA GAGCGCTGAC 
TCTAAGTCAG AGACGCCTGA CCCTACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
GTGCCTCCAA GGGTTCCTGT GAGAACAACG TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT 
TCCCCACTGC AGGGCAGTGG GCAGCAGAAT AGCCAGGCAG GACAGAGAAA CTCCACCAGC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCGTCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG ACAACGCAGT GAAAAAACCT GAAGATAAAA AAGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC GGTGGAAGAT 
GTACGGCCCC CTCACAAAGT GACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCCGACG AGTCCACCTC GGGACCGGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC 
TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGTGGAACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCGGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ATACCCCAGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAAATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTGATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAAAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCCGGATCAG TCTATGACAT TTATCTACCA 
ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC 
ATCATCCTCC CCAATACAGA TGGAATGGAG CTTTTGGTGT GCTATGAAGA TGAGGGGGTT 
TATGTAAACA CATATGGAAG GATCACCAAG GATGTAGTTC TACAGTGGGG AGAGATGCCT 
ACGTCAGTAG CATATATTCG ATCCAATCAG ACAATGGGCT GGGGAGAGAA GGCCATAGAG 
ATCCGATCTG TGGAAACTGG TCACTTGGAT GGTGTGTTCA TGCACAAAAG GGCTCAAAGA 
CTAAAATTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC 
AGCAGTCAGG TTTATTTCAT GACCTTAGGC AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008219.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006410.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCh101922
Clone ID Related Accession (Same CDS sequence)	XM_008008219.1
Accession Version	XM_008008219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGTG GAACAACAGT GACTTCTGTG 
GTGGGATTTT CCTGTGATGG GATGAGACCG GAAGCCATAA GGCAAGATCC TACCCGGAAA 
GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGATAC CCCAGAGATT 
CGTAAATACA AGAAGAGGTT TAACTCTGAA ATTCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC 
TATCCTCTGA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG 
GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG 
ATTGCTTTGA AAAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCCG GATCAGTCTA TGACATTTAT CTACCAACAC ACGTAAGAAA GAACCCACAC 
TCTATGATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTT TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACGT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006410.1
CDS	178..4167
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008219.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008219.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGTG GAACAACAGT GACTTCTGTG 
GTGGGATTTT CCTGTGATGG GATGAGACCG GAAGCCATAA GGCAAGATCC TACCCGGAAA 
GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGATAC CCCAGAGATT 
CGTAAATACA AGAAGAGGTT TAACTCTGAA ATTCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC 
TATCCTCTGA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG 
GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG 
ATTGCTTTGA AAAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCCG GATCAGTCTA TGACATTTAT CTACCAACAC ACGTAAGAAA GAACCCACAC 
TCTATGATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTT TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACGT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008220.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006411.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

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CloneID	OCh101923
Clone ID Related Accession (Same CDS sequence)	XM_008008220.1
Accession Version	XM_008008220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGTG GAACAACAGT GACTTCTGTG 
GTGGGATTTT CCTGTGATGG GATGAGACCG GAAGCCATAA GGCAAGATCC TACCCGGAAA 
GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGATAC CCCAGAGATT 
CGTAAATACA AGAAGAGGTT TAACTCTGAA ATTCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC 
TATCCTCTGA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG 
GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG 
ATTGCTTTGA AAAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCCG GATCAGTCTA TGACATTTAT CTACCAACAC ACATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTTTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACGTCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006411.1
CDS	178..4143
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008220.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008220.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGCCCCA CCTGGCATCT GTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCTCCC AAATTTGCAC ACCACCATCT TCGTTCTCAG 
GACCCATGTC CACCTTCCCG CAGTGAGGTG CTCGGTCAGA GCGCTGACTC TAAGTCAGAG 
ACGCCTGACC CTACCCAAAA GGCTTGGTCT AGATCAGACA GTGACGAGGT GCCTCCAAGG 
GTTCCTGTGA GAACAACGTC TCGCTCCCCT GTTCTGTCCC GTCGAGATTC CCCACTGCAG 
GGCAGTGGGC AGCAGAATAG CCAGGCAGGA CAGAGAAACT CCACCAGCAG TATTGAGCCC 
AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA 
GGGTCCAGCA ACTCAGGATC CCAGCCCGGG TCTCACCCTG GGTCTCAGAG TGGCTCCGGG 
GAACGCTTCA GAGTGAGATC GTCATCCAAG TCTGAAGGCT CTCCATCTCA GCGCCTGGAC 
AACGCAGTGA AAAAACCTGA AGATAAAAAA GAAGTTTTCA GACCCCTCAA GCCTGCTGGC 
GAAGTGGATC TGACCGCACT GGCCAAAGAG CTTCGAGCGG TGGAAGATGT ACGGCCCCCT 
CACAAAGTGA CGGACTACTC CTCATCCAGT GAGGAGTCGG GGACGACGGA TGAGGAGGAC 
GACGATGTGG AGCAGGAAGG GGCCGACGAG TCCACCTCGG GACCGGAGGA CACCAGAGCA 
GCGTCATCTC TGAATTTGAG CAATGGTGAA ACGGAATCTG TGAAAACCAT GATTGTCCAT 
GATGATGTAG AAAGTGAGCC GGCCATGACC CCATCCAAGG AGGGCACTCT AATCGTCCGC 
CAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGTG GAACAACAGT GACTTCTGTG 
GTGGGATTTT CCTGTGATGG GATGAGACCG GAAGCCATAA GGCAAGATCC TACCCGGAAA 
GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGATAC CCCAGAGATT 
CGTAAATACA AGAAGAGGTT TAACTCTGAA ATTCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC 
TATCCTCTGA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG 
GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG 
ATTGCTTTGA AAAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCCG GATCAGTCTA TGACATTTAT CTACCAACAC ACATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTTTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACGTCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008221.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006412.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

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CloneID	OCh101924
Clone ID Related Accession (Same CDS sequence)	XM_008008221.1
Accession Version	XM_008008221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACGTCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCGTCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGACAACG CAGTGAAAAA ACCTGAAGAT AAAAAAGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCGGTGGA AGATGTACGG 
CCCCCTCACA AAGTGACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCC GACGAGTCCA CCTCGGGACC GGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGA 
ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCGGA AGCCATAAGG 
CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG 
AGTGATACCC CAGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAAAT TCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTCTA TCCTCTGATC AACCGAAGAC GATTTCAACA AATGGACGTA 
CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG 
GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA 
AGAATCAAAT TTCTGGTGAT TGCTTTGAAA AGTTCTGTGG AAGTCTATGC GTGGGCACCA 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCCGGA TCAGTCTATG ACATTTATCT ACCAACACAC 
GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTTTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACGTCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006412.1
CDS	178..4125
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008221.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008221.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACGTCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCGTCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGACAACG CAGTGAAAAA ACCTGAAGAT AAAAAAGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCGGTGGA AGATGTACGG 
CCCCCTCACA AAGTGACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCC GACGAGTCCA CCTCGGGACC GGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGTGGA 
ACAACAGTGA CTTCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCGGA AGCCATAAGG 
CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG 
AGTGATACCC CAGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAAAT TCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTCTA TCCTCTGATC AACCGAAGAC GATTTCAACA AATGGACGTA 
CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG 
GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA 
AGAATCAAAT TTCTGGTGAT TGCTTTGAAA AGTTCTGTGG AAGTCTATGC GTGGGCACCA 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCCGGA TCAGTCTATG ACATTTATCT ACCAACACAC 
GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTTTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACGTCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008222.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006413.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCh101925
Clone ID Related Accession (Same CDS sequence)	XM_008008222.1
Accession Version	XM_008008222.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTACAGTGG 
CCCCACCTGG CATCTGTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGTT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTCGG TCAGAGCGCT GACTCTAAGT CAGAGACGCC TGACCCTACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACA 
ACGTCTCGCT CCCCTGTTCT GTCCCGTCGA GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
AATAGCCAGG CAGGACAGAG AAACTCCACC AGCAGTATTG AGCCCAGGCT TCTGTGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGACCTGGC AGTGGCAGCT CCTCAGGGTC CAGCAACTCA 
GGATCCCAGC CCGGGTCTCA CCCTGGGTCT CAGAGTGGCT CCGGGGAACG CTTCAGAGTG 
AGATCGTCAT CCAAGTCTGA AGGCTCTCCA TCTCAGCGCC TGGACAACGC AGTGAAAAAA 
CCTGAAGATA AAAAAGAAGT TTTCAGACCC CTCAAGCCTG CTGGCGAAGT GGATCTGACC 
GCACTGGCCA AAGAGCTTCG AGCGGTGGAA GATGTACGGC CCCCTCACAA AGTGACGGAC 
TACTCCTCAT CCAGTGAGGA GTCGGGGACG ACGGATGAGG AGGACGACGA TGTGGAGCAG 
GAAGGGGCCG ACGAGTCCAC CTCGGGACCG GAGGACACCA GAGCAGCGTC ATCTCTGAAT 
TTGAGCAATG GTGAAACGGA ATCTGTGAAA ACCATGATTG TCCATGATGA TGTAGAAAGT 
GAGCCGGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGAA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGGAT GAGACCGGAA GCCATAAGGC AAGATCCTAC CCGGAAAGGC 
TCAGTGGTCA ATGTGAATCC TACCAACACT AGGCCACAGA GTGATACCCC AGAGATTCGT 
AAATACAAGA AGAGGTTTAA CTCTGAAATT CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTCTAT 
CCTCTGATCA ACCGAAGACG ATTTCAACAA ATGGACGTAC TTGAGGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAATGATCC AGAAGTTGAG AAGAAGCAGG GATGGACAAC CGTAGGGGAT 
TTGGAAGGAT GTGTACATTA TAAAGTTGTA AAATATGAAA GAATCAAATT TCTGGTGATT 
GCTTTGAAAA GTTCTGTGGA AGTCTATGCG TGGGCACCAA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ATTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGAT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAATAC AGATGGAATG 
GAGCTTTTGG TGTGCTATGA AGATGAGGGG GTTTATGTAA ACACATATGG AAGGATCACC 
AAGGATGTAG TTCTACAGTG GGGAGAGATG CCTACGTCAG TAGCATATAT TCGATCCAAT 
CAGACAATGG GCTGGGGAGA GAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCACTTG 
GATGGTGTGT TCATGCACAA AAGGGCTCAA AGACTAAAAT TCTTGTGTGA ACGCAATGAC 
AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGTC AGGTTTATTT CATGACCTTA 
GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006413.1
CDS	178..4104
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008222.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008222.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTACAGTGG 
CCCCACCTGG CATCTGTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGTT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTCGG TCAGAGCGCT GACTCTAAGT CAGAGACGCC TGACCCTACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACA 
ACGTCTCGCT CCCCTGTTCT GTCCCGTCGA GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
AATAGCCAGG CAGGACAGAG AAACTCCACC AGCAGTATTG AGCCCAGGCT TCTGTGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGACCTGGC AGTGGCAGCT CCTCAGGGTC CAGCAACTCA 
GGATCCCAGC CCGGGTCTCA CCCTGGGTCT CAGAGTGGCT CCGGGGAACG CTTCAGAGTG 
AGATCGTCAT CCAAGTCTGA AGGCTCTCCA TCTCAGCGCC TGGACAACGC AGTGAAAAAA 
CCTGAAGATA AAAAAGAAGT TTTCAGACCC CTCAAGCCTG CTGGCGAAGT GGATCTGACC 
GCACTGGCCA AAGAGCTTCG AGCGGTGGAA GATGTACGGC CCCCTCACAA AGTGACGGAC 
TACTCCTCAT CCAGTGAGGA GTCGGGGACG ACGGATGAGG AGGACGACGA TGTGGAGCAG 
GAAGGGGCCG ACGAGTCCAC CTCGGGACCG GAGGACACCA GAGCAGCGTC ATCTCTGAAT 
TTGAGCAATG GTGAAACGGA ATCTGTGAAA ACCATGATTG TCCATGATGA TGTAGAAAGT 
GAGCCGGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGAA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGGAT GAGACCGGAA GCCATAAGGC AAGATCCTAC CCGGAAAGGC 
TCAGTGGTCA ATGTGAATCC TACCAACACT AGGCCACAGA GTGATACCCC AGAGATTCGT 
AAATACAAGA AGAGGTTTAA CTCTGAAATT CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTCTAT 
CCTCTGATCA ACCGAAGACG ATTTCAACAA ATGGACGTAC TTGAGGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAATGATCC AGAAGTTGAG AAGAAGCAGG GATGGACAAC CGTAGGGGAT 
TTGGAAGGAT GTGTACATTA TAAAGTTGTA AAATATGAAA GAATCAAATT TCTGGTGATT 
GCTTTGAAAA GTTCTGTGGA AGTCTATGCG TGGGCACCAA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ATTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGAT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
ATGATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAATAC AGATGGAATG 
GAGCTTTTGG TGTGCTATGA AGATGAGGGG GTTTATGTAA ACACATATGG AAGGATCACC 
AAGGATGTAG TTCTACAGTG GGGAGAGATG CCTACGTCAG TAGCATATAT TCGATCCAAT 
CAGACAATGG GCTGGGGAGA GAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCACTTG 
GATGGTGTGT TCATGCACAA AAGGGCTCAA AGACTAAAAT TCTTGTGTGA ACGCAATGAC 
AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGTC AGGTTTATTT CATGACCTTA 
GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008223.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006414.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCh101926
Clone ID Related Accession (Same CDS sequence)	XM_008008223.1
Accession Version	XM_008008223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTACAGTGG 
CCCCACCTGG CATCTGTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGTT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTCGG TCAGAGCGCT GACTCTAAGT CAGAGACGCC TGACCCTACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACA 
ACGTCTCGCT CCCCTGTTCT GTCCCGTCGA GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
AATAGCCAGG CAGGACAGAG AAACTCCACC AGCAGTATTG AGCCCAGGCT TCTGTGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGACCTGGC AGTGGCAGCT CCTCAGGGTC CAGCAACTCA 
GGATCCCAGC CCGGGTCTCA CCCTGGGTCT CAGAGTGGCT CCGGGGAACG CTTCAGAGTG 
AGATCGTCAT CCAAGTCTGA AGGCTCTCCA TCTCAGCGCC TGGACAACGC AGTGAAAAAA 
CCTGAAGATA AAAAAGAAGT TTTCAGACCC CTCAAGCCTG CTGGCGAAGT GGATCTGACC 
GCACTGGCCA AAGAGCTTCG AGCGGTGGAA GATGTACGGC CCCCTCACAA AGTGACGGAC 
TACTCCTCAT CCAGTGAGGA GTCGGGGACG ACGGATGAGG AGGACGACGA TGTGGAGCAG 
GAAGGGGCCG ACGAGTCCAC CTCGGGACCG GAGGACACCA GAGCAGCGTC ATCTCTGAAT 
TTGAGCAATG GTGAAACGGA ATCTGTGAAA ACCATGATTG TCCATGATGA TGTAGAAAGT 
GAGCCGGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGAA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGGAT GAGACCGGAA GCCATAAGGC AAGATCCTAC CCGGAAAGGC 
TCAGTGGTCA ATGTGAATCC TACCAACACT AGGCCACAGA GTGATACCCC AGAGATTCGT 
AAATACAAGA AGAGGTTTAA CTCTGAAATT CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTCTAT 
CCTCTGATCA ACCGAAGACG ATTTCAACAA ATGGACGTAC TTGAGGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAATGATCC AGAAGTTGAG AAGAAGCAGG GATGGACAAC CGTAGGGGAT 
TTGGAAGGAT GTGTACATTA TAAAGTTGTA AAATATGAAA GAATCAAATT TCTGGTGATT 
GCTTTGAAAA GTTCTGTGGA AGTCTATGCG TGGGCACCAA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ATTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGAT CAGTCTATGA CATTTATCTA CCAACACACA TCCAGTGTAG CATCAAACCC 
CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTTTGGTGTG CTATGAAGAT 
GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA 
GAGATGCCTA CGTCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG 
GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG 
GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG 
TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006414.1
CDS	178..4080
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008223.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008223.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTACAGTGG 
CCCCACCTGG CATCTGTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGTT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTCGG TCAGAGCGCT GACTCTAAGT CAGAGACGCC TGACCCTACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACA 
ACGTCTCGCT CCCCTGTTCT GTCCCGTCGA GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
AATAGCCAGG CAGGACAGAG AAACTCCACC AGCAGTATTG AGCCCAGGCT TCTGTGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGACCTGGC AGTGGCAGCT CCTCAGGGTC CAGCAACTCA 
GGATCCCAGC CCGGGTCTCA CCCTGGGTCT CAGAGTGGCT CCGGGGAACG CTTCAGAGTG 
AGATCGTCAT CCAAGTCTGA AGGCTCTCCA TCTCAGCGCC TGGACAACGC AGTGAAAAAA 
CCTGAAGATA AAAAAGAAGT TTTCAGACCC CTCAAGCCTG CTGGCGAAGT GGATCTGACC 
GCACTGGCCA AAGAGCTTCG AGCGGTGGAA GATGTACGGC CCCCTCACAA AGTGACGGAC 
TACTCCTCAT CCAGTGAGGA GTCGGGGACG ACGGATGAGG AGGACGACGA TGTGGAGCAG 
GAAGGGGCCG ACGAGTCCAC CTCGGGACCG GAGGACACCA GAGCAGCGTC ATCTCTGAAT 
TTGAGCAATG GTGAAACGGA ATCTGTGAAA ACCATGATTG TCCATGATGA TGTAGAAAGT 
GAGCCGGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAG TACAGTTGAC 
CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCCG GTCGCATTCA CCTCTTGCCA 
GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCCTC CCCATCCTCC 
AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG 
ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGTGGAA CAACAGTGAC TTCTGTGGTG 
GGATTTTCCT GTGATGGGAT GAGACCGGAA GCCATAAGGC AAGATCCTAC CCGGAAAGGC 
TCAGTGGTCA ATGTGAATCC TACCAACACT AGGCCACAGA GTGATACCCC AGAGATTCGT 
AAATACAAGA AGAGGTTTAA CTCTGAAATT CTGTGTGCTG CCTTATGGGG AGTGAATTTG 
CTAGTGGGTA CAGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTCTAT 
CCTCTGATCA ACCGAAGACG ATTTCAACAA ATGGACGTAC TTGAGGGCTT GAATGTCTTG 
GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
AAAATACTTC ACAATGATCC AGAAGTTGAG AAGAAGCAGG GATGGACAAC CGTAGGGGAT 
TTGGAAGGAT GTGTACATTA TAAAGTTGTA AAATATGAAA GAATCAAATT TCTGGTGATT 
GCTTTGAAAA GTTCTGTGGA AGTCTATGCG TGGGCACCAA AGCCATATCA CAAATTTATG 
GCCTTTAAGT CATTTGGAGA ATTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTTGAG 
GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
GATTCCGGAT CAGTCTATGA CATTTATCTA CCAACACACA TCCAGTGTAG CATCAAACCC 
CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTTTGGTGTG CTATGAAGAT 
GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA 
GAGATGCCTA CGTCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG 
GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG 
GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG 
TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008224.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006415.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

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CloneID	OCh101927
Clone ID Related Accession (Same CDS sequence)	XM_008008224.1
Accession Version	XM_008008224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGCCCCACCT GGCATCTGTC 
AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
TTTGCACACC ACCATCTTCG TTCTCAGGAC CCATGTCCAC CTTCCCGCAG TGAGGTGCTC 
GGTCAGAGCG CTGACTCTAA GTCAGAGACG CCTGACCCTA CCCAAAAGGC TTGGTCTAGA 
TCAGACAGTG ACGAGGTGCC TCCAAGGGTT CCTGTGAGAA CAACGTCTCG CTCCCCTGTT 
CTGTCCCGTC GAGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAATAGCCA GGCAGGACAG 
AGAAACTCCA CCAGCAGTAT TGAGCCCAGG CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG 
CCCAGACCTG GCAGTGGCAG CTCCTCAGGG TCCAGCAACT CAGGATCCCA GCCCGGGTCT 
CACCCTGGGT CTCAGAGTGG CTCCGGGGAA CGCTTCAGAG TGAGATCGTC ATCCAAGTCT 
GAAGGCTCTC CATCTCAGCG CCTGGACAAC GCAGTGAAAA AACCTGAAGA TAAAAAAGAA 
GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA GTGGATCTGA CCGCACTGGC CAAAGAGCTT 
CGAGCGGTGG AAGATGTACG GCCCCCTCAC AAAGTGACGG ACTACTCCTC ATCCAGTGAG 
GAGTCGGGGA CGACGGATGA GGAGGACGAC GATGTGGAGC AGGAAGGGGC CGACGAGTCC 
ACCTCGGGAC CGGAGGACAC CAGAGCAGCG TCATCTCTGA ATTTGAGCAA TGGTGAAACG 
GAATCTGTGA AAACCATGAT TGTCCATGAT GATGTAGAAA GTGAGCCGGC CATGACCCCA 
TCCAAGGAGG GCACTCTAAT CGTCCGCCAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGTGGAA CAACAGTGAC TTCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCGGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGATACCCC AGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAAATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTGATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAAA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCCGGAT CAGTCTATGA CATTTATCTA 
CCAACACACA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTTTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CGTCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006415.1
CDS	178..4050
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008224.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008224.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGCCCCACCT GGCATCTGTC 
AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
TTTGCACACC ACCATCTTCG TTCTCAGGAC CCATGTCCAC CTTCCCGCAG TGAGGTGCTC 
GGTCAGAGCG CTGACTCTAA GTCAGAGACG CCTGACCCTA CCCAAAAGGC TTGGTCTAGA 
TCAGACAGTG ACGAGGTGCC TCCAAGGGTT CCTGTGAGAA CAACGTCTCG CTCCCCTGTT 
CTGTCCCGTC GAGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAATAGCCA GGCAGGACAG 
AGAAACTCCA CCAGCAGTAT TGAGCCCAGG CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG 
CCCAGACCTG GCAGTGGCAG CTCCTCAGGG TCCAGCAACT CAGGATCCCA GCCCGGGTCT 
CACCCTGGGT CTCAGAGTGG CTCCGGGGAA CGCTTCAGAG TGAGATCGTC ATCCAAGTCT 
GAAGGCTCTC CATCTCAGCG CCTGGACAAC GCAGTGAAAA AACCTGAAGA TAAAAAAGAA 
GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA GTGGATCTGA CCGCACTGGC CAAAGAGCTT 
CGAGCGGTGG AAGATGTACG GCCCCCTCAC AAAGTGACGG ACTACTCCTC ATCCAGTGAG 
GAGTCGGGGA CGACGGATGA GGAGGACGAC GATGTGGAGC AGGAAGGGGC CGACGAGTCC 
ACCTCGGGAC CGGAGGACAC CAGAGCAGCG TCATCTCTGA ATTTGAGCAA TGGTGAAACG 
GAATCTGTGA AAACCATGAT TGTCCATGAT GATGTAGAAA GTGAGCCGGC CATGACCCCA 
TCCAAGGAGG GCACTCTAAT CGTCCGCCAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGTGGAA CAACAGTGAC TTCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCGGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGATACCCC AGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAAATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTGATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAAA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCCGGAT CAGTCTATGA CATTTATCTA 
CCAACACACA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTTTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CGTCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008226.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006417.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCh101928
Clone ID Related Accession (Same CDS sequence)	XM_008008226.1
Accession Version	XM_008008226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC GTCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCGTCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GACAACGCAG TGAAAAAACC TGAAGATAAA 
AAAGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGTGGAAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCGGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGATACCCCA GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAAATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTGATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAAAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGATC AGTCTATGAC 
ATTTATCTAC CAACACACAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TTTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC GTCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006417.1
CDS	177..3998
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008226.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008226.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC GTCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCGTCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GACAACGCAG TGAAAAAACC TGAAGATAAA 
AAAGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGTGGAAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCGGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGATACCCCA GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAAATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTGATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAAAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGATC AGTCTATGAC 
ATTTATCTAC CAACACACAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TTTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC GTCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008227.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006418.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCh101929
Clone ID Related Accession (Same CDS sequence)	XM_008008227.1
Accession Version	XM_008008227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CGTCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCGTCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGACAACGCA GTGAAAAAAC CTGAAGATAA AAAAGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGTGGAAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCGGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGATACCCCA GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAAATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTGATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAAAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGATC AGTCTATGAC 
ATTTATCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTTTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACGTCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006418.1
CDS	178..3963
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008227.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008227.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CGTCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCGTCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGACAACGCA GTGAAAAAAC CTGAAGATAA AAAAGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGTGGAAC AACAGTGACT TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCGGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGATACCCCA GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAAATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTGATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAAAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGATC AGTCTATGAC 
ATTTATCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTTTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACGTCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008228.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006419.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

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CloneID	OCh101930
Clone ID Related Accession (Same CDS sequence)	XM_008008228.1
Accession Version	XM_008008228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACGTCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCGTCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GACAACGCAG TGAAAAAACC TGAAGATAAA AAAGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCGGTGGAAG ATGTACGGCC CCCTCACAAA 
GTGACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCCGA CGAGTCCACC TCGGGACCGG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGTGGAACAA CAGTGACTTC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCGGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ATACCCCAGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAAATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTGATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAAAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCCGGATCAG TCTATGACAT TTATCTACCA ACACACATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTT TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACGT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006419.1
CDS	178..3897
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008228.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008228.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCGC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACGTCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCGTCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GACAACGCAG TGAAAAAACC TGAAGATAAA AAAGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCGGTGGAAG ATGTACGGCC CCCTCACAAA 
GTGACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCCGA CGAGTCCACC TCGGGACCGG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGTGGAACAA CAGTGACTTC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCGGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ATACCCCAGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAAATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTGATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAAAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCCGGATCAG TCTATGACAT TTATCTACCA ACACACATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTT TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACGT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008229.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006420.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OCh101931
Clone ID Related Accession (Same CDS sequence)	XM_008008229.1
Accession Version	XM_008008229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTACAGTGG 
CCCCACCTGG CATCTGTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGTT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTCGG TCAGAGCGCT GACTCTAAGT CAGAGACGCC TGACCCTACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACA 
ACGTCTCGCT CCCCTGTTCT GTCCCGTCGA GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
AATAGCCAGG CAGGACAGAG AAACTCCACC AGCAGTATTG AGCCCAGGCT TCTGTGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGACCTGGC AGTGGCAGCT CCTCAGGGTC CAGCAACTCA 
GGATCCCAGC CCGGGTCTCA CCCTGGGTCT CAGAGTGGCT CCGGGGAACG CTTCAGAGTG 
AGATCGTCAT CCAAGTCTGA AGGCTCTCCA TCTCAGCGCC TGGACAACGC AGTGAAAAAA 
CCTGAAGATA AAAAAGAAGT TTTCAGACCC CTCAAGCCTG CTGGCGAAGT GGATCTGACC 
GCACTGGCCA AAGAGCTTCG AGCGGTGGAA GATGTACGGC CCCCTCACAA AGTGACGGAC 
TACTCCTCAT CCAGTGAGGA GTCGGGGACG ACGGATGAGG AGGACGACGA TGTGGAGCAG 
GAAGGGGCCG ACGAGTCCAC CTCGGGACCG GAGGACACCA GAGCAGCGTC ATCTCTGAAT 
TTGAGCAATG GTGAAACGGA ATCTGTGAAA ACCATGATTG TCCATGATGA TGTAGAAAGT 
GAGCCGGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGTGGAACA ACAGTGACTT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCGGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GATACCCCAG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAAATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTGATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAAAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCCGGATCA 
GTCTATGACA TTTATCTACC AACACACATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT TTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACG 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006420.1
CDS	178..3888
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVQWPHLASVKNNVSPVSRSHSFSDPSPPKFAHHHLRSQDPCPPSRSEVLGQSADSKSETPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008229.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008229.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTACAGTGG 
CCCCACCTGG CATCTGTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGTT CTCAGGACCC ATGTCCACCT 
TCCCGCAGTG AGGTGCTCGG TCAGAGCGCT GACTCTAAGT CAGAGACGCC TGACCCTACC 
CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACA 
ACGTCTCGCT CCCCTGTTCT GTCCCGTCGA GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
AATAGCCAGG CAGGACAGAG AAACTCCACC AGCAGTATTG AGCCCAGGCT TCTGTGGGAG 
AGAGTGGAGA AGCTGGTGCC CAGACCTGGC AGTGGCAGCT CCTCAGGGTC CAGCAACTCA 
GGATCCCAGC CCGGGTCTCA CCCTGGGTCT CAGAGTGGCT CCGGGGAACG CTTCAGAGTG 
AGATCGTCAT CCAAGTCTGA AGGCTCTCCA TCTCAGCGCC TGGACAACGC AGTGAAAAAA 
CCTGAAGATA AAAAAGAAGT TTTCAGACCC CTCAAGCCTG CTGGCGAAGT GGATCTGACC 
GCACTGGCCA AAGAGCTTCG AGCGGTGGAA GATGTACGGC CCCCTCACAA AGTGACGGAC 
TACTCCTCAT CCAGTGAGGA GTCGGGGACG ACGGATGAGG AGGACGACGA TGTGGAGCAG 
GAAGGGGCCG ACGAGTCCAC CTCGGGACCG GAGGACACCA GAGCAGCGTC ATCTCTGAAT 
TTGAGCAATG GTGAAACGGA ATCTGTGAAA ACCATGATTG TCCATGATGA TGTAGAAAGT 
GAGCCGGCCA TGACCCCATC CAAGGAGGGC ACTCTAATCG TCCGCCAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGTGGAACA ACAGTGACTT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCGGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GATACCCCAG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAAATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTGATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAAAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCCGGATCA 
GTCTATGACA TTTATCTACC AACACACATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT TTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACG 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008230.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006421.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OCh101932
Clone ID Related Accession (Same CDS sequence)	XM_008008230.1
Accession Version	XM_008008230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACGT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CGTCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA CAACGCAGTG 
AAAAAACCTG AAGATAAAAA AGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCG GTGGAAGATG TACGGCCCCC TCACAAAGTG 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCCGACGA GTCCACCTCG GGACCGGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGAACAAC AGTGACTTCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCGGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA TACCCCAGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAAATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TGATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAAAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGATCAGT CTATGACATT TATCTACCAA CACACATCCA GTGTAGCATC 
AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTTT GGTGTGCTAT 
GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG 
TGGGGAGAGA TGCCTACGTC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC 
AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT 
GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006421.1
CDS	177..3845
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008230.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008230.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACGT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CGTCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA CAACGCAGTG 
AAAAAACCTG AAGATAAAAA AGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCG GTGGAAGATG TACGGCCCCC TCACAAAGTG 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCCGACGA GTCCACCTCG GGACCGGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGAACAAC AGTGACTTCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCGGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA TACCCCAGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAAATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TGATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAAAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CCGGATCAGT CTATGACATT TATCTACCAA CACACATCCA GTGTAGCATC 
AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTTT GGTGTGCTAT 
GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG 
TGGGGAGAGA TGCCTACGTC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC 
AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT 
GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008231.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006422.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

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CloneID	OCh101933
Clone ID Related Accession (Same CDS sequence)	XM_008008231.1
Accession Version	XM_008008231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC GTCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCGTCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GACAACGCAG TGAAAAAACC TGAAGATAAA 
AAAGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CGGTGGAAGA TGTACGGCCC CCTCACAAAG TGACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCCGAC 
GAGTCCACCT CGGGACCGGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GTGGAACAAC AGTGACTTCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCGGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA TACCCCAGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAAATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TGATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAAAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CCGGATCAGT CTATGACATT 
TATCTACCAA CACACATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTTT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACGTC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006422.1
CDS	177..3815
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008231.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008231.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC GTCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCGTCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GACAACGCAG TGAAAAAACC TGAAGATAAA 
AAAGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CGGTGGAAGA TGTACGGCCC CCTCACAAAG TGACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCCGAC 
GAGTCCACCT CGGGACCGGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GTGGAACAAC AGTGACTTCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCGGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA TACCCCAGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAAATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TGATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAAAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CCGGATCAGT CTATGACATT 
TATCTACCAA CACACATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTTT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACGTC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008232.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006423.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OCh101934
Clone ID Related Accession (Same CDS sequence)	XM_008008232.1
Accession Version	XM_008008232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC GTCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCGTCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GACAACGCAG TGAAAAAACC TGAAGATAAA 
AAAGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGTGGAACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCGGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ATACCCCAGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAAATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTGATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAAAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCCGGATCAG TCTATGACAT TTATCTACCA 
ACACACATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTT TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACGT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006423.1
CDS	177..3806
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008232.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008232.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCGCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC GTCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCGTCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GACAACGCAG TGAAAAAACC TGAAGATAAA 
AAAGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGTGGAACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCGGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ATACCCCAGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAAATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTGATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAAAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCCGGATCAG TCTATGACAT TTATCTACCA 
ACACACATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTT TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACGT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008233.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006424.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.

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CloneID	OCh101935
Clone ID Related Accession (Same CDS sequence)	XM_008008233.1
Accession Version	XM_008008233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CGTCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCGTCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGACAACGCA GTGAAAAAAC CTGAAGATAA AAAAGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGTGGAACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCGGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ATACCCCAGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAAATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTGATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAAAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCCGGATCAG TCTATGACAT TTATCTACCA 
ACACACATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTT TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACGT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006424.1
CDS	177..3746
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008233.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008233.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCACA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGTCCACTAC 
GAGCCTGCCG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CGTCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCGTCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGACAACGCA GTGAAAAAAC CTGAAGATAA AAAAGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCGGTGGAAG ATGTACGGCC CCCTCACAAA GTGACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCCGA CGAGTCCACC 
TCGGGACCGG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGTGGAACAA CAGTGACTTC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCGGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ATACCCCAGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAAATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTGATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAAAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCCGGATCAG TCTATGACAT TTATCTACCA 
ACACACATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTT TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACGT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_008008234.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_008006425.1 Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.

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CloneID	OCh101936
Clone ID Related Accession (Same CDS sequence)	XM_008008234.1
Accession Version	XM_008008234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-13
Organism	Chlorocebus sabaeus(green monkey)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735259.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACGT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCGT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGACAA CGCAGTGAAA 
AAACCTGAAG ATAAAAAAGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCGGTG GAAGATGTAC GGCCCCCTCA CAAAGTGACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CCGACGAGTC CACCTCGGGA CCGGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGATTACTAC AGATTTCTCC ATCTAGTGGA ACAACAGTGA CTTCTGTGGT GGGATTTTCC 
TGTGATGGGA TGAGACCGGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC 
AATGTGAATC CTACCAACAC TAGGCCACAG AGTGATACCC CAGAGATTCG TAAATACAAG 
AAGAGGTTTA ACTCTGAAAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT 
ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTGATC 
AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT 
CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA 
TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAA 
AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGA 
TCAGTCTATG ACATTTATCT ACCAACACAC GTAAGAAAGA ACCCACACTC TATGATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTTTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACGTCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008006425.1
CDS	178..3675
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYTQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKVHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLDNAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_008008234.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008008234.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ATCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AATATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATT GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCACGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACAGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TCGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAATTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACACGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACGAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGTC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC TGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCGG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG TGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTCCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCACAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGT CCACTACGAG CCTGCCGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACGT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCGT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGACAA CGCAGTGAAA 
AAACCTGAAG ATAAAAAAGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCGGTG GAAGATGTAC GGCCCCCTCA CAAAGTGACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CCGACGAGTC CACCTCGGGA CCGGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGATTACTAC AGATTTCTCC ATCTAGTGGA ACAACAGTGA CTTCTGTGGT GGGATTTTCC 
TGTGATGGGA TGAGACCGGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC 
AATGTGAATC CTACCAACAC TAGGCCACAG AGTGATACCC CAGAGATTCG TAAATACAAG 
AAGAGGTTTA ACTCTGAAAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT 
ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTGATC 
AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT 
CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA 
TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAA 
AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGA 
TCAGTCTATG ACATTTATCT ACCAACACAC GTAAGAAAGA ACCCACACTC TATGATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTTTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACGTCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAP4K4
Entrez Gene ID	103241142
Full Name	mitogen-activated protein kinase kinase kinase kinase 4
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAP4K4 cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

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