LOC109712220 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109712220
Entrez Gene ID	109712220
Full Name	putative disease resistance protein RGA3
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020235683.1	XP_020091272.1	putative disease resistance protein RGA3
XM_020235675.1	XP_020091264.1	putative disease resistance protein RGA3

Pathways from BioSystems
Gene Ontology

The following LOC109712220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109712220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn58784	XM_020235675.1 Latest version!	Ananas comosus putative disease resistance protein RGA3 (LOC109712220), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAn58784	XM_020235683.1 Latest version!	Ananas comosus putative disease resistance protein RGA3 (LOC109712220), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109712220 ( XM_020235683.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020091272.1 Ananas comosus putative disease resistance protein RGA3 (LOC109712220), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn58784
Clone ID Related Accession (Same CDS sequence)	XM_020235683.1 , XM_020235675.1
Accession Version	XM_020235683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	putative disease resistance protein RGA3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033621.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCT TAGTCGACGA ACCAATAGTA TATGGTCGAG ACGAGGAACG GGAGAAGGTG 
ATCAAGTTGT TGGGAGTTCC ATGCGGGCAG AGTGACATTG AAAACGACAA TGAAAGTGTA 
TCTGCCCCTG CTAAGAGAAG AAAGAAAGAA AATGTGTCTG TTCTATCCAT TTATGGCATC 
GGAGGGGTCG GCAAGACTAC TCTAGCACAG GTTGTCTACA ATGATGAGAG GGTGGTGAAG 
CACTTTGACC TGAAAATTTG GGTCTGTGTT TCTGACAACT TCGATGTGAA CAGCTTAAGT 
AAAGAGATCA TAGATAACGC TGGGAAGGGG TACGAGGCTG ATCGTATGAA TTTAAGTTCT 
CTTCAAGCGA CTCTAAAGGA GATAGTGATG GCACATCGCT TCTTGCTTGT CCTTGATGAT 
GTGTGGAATG AGAATAGTAG GGAGTGGCAA AAATTTTATG CGCCTTTGAG CCATGGGCAA 
GTAGGAAGTA TGATCCTGGT GACGACTCGG ACTTTGAAGG TTGCCAACAT AGCAGGAACA 
ATGGACCCCA TCTTCCTGGA GGGTTTATCA GGTGATGTTT ATTGGGATTT CTTCAAGTCA 
TGCGCATTTC GCTGTGAGAA CCCTGAAGAC TATCCGGGGT TAGAAAAAAT AGGAATAGAC 
ATTGCTGCTA AGTTGAAGGG ATCTCCGTTA GCTGCAAAAA CTTTAGGAGG CCTGTTGAAT 
GAGGACATGG ATGCCGGACA TTGGACTACG ATCTTGAACA GTGAATTATG GGAACTACCG 
CAGAAGGAAG GCGACATTCT TCCAGCCCTT CGATTGAGCT ATCTGTATCT TCTGCCGCAT 
CTGAAGCGAT GCTTCTCGTT TTGTTCGATA TTCCCAAAAG ATTATGAGTT CAATAAAAGT 
GATCTAACTA GTATTTGGGT AGCAGAAGGC TTTGTTATAC CTCAAGGAAA CATGCTACTA 
GAAGATATAG CGACCATGTA CTTGCGCGAC TTAACAAGCA GGTGCTTCCT TCAGCAATCT 
CATAGGGATC CTCTAAACTA TGTAATGCAT GATTTGATAC ACGATCTAGC GCAATTTGTT 
TCGGTAGACG AATGTTCAAG GATAGATAAA GGTAGAGGAC AAATAAGAAT CCCTAAAACT 
GTTCGACATT TGTCGGTATG CTGTACGAGT ATAGAAGAAA AGAAGTTAAT GGAGTTAGCG 
AGCTACGACA AACTGCGCTC GATTATAATA CTTTCTAAAT TTCGGCGTGG ATTAGATTCT 
ATAATTGATT GCTGGTTCCG CAGGCTCGCA AATATCCGTT GTTTAAAACT TAGCTTCTGT 
AAGATAACAA AGCTTCCTGA GAGTATTAGC AACTTAAAGC ATCTCCGATA CCTTGATGTA 
TCTGGTACAA GTATCAGAAC GTTACCCACG TCACTGTGTC ACCTCAATAA TCTGCAAGTG 
TTGAACGTAA TGAAGTGCCC GCTTGAGTGT TTTCCTCAAG GATTTACTGG ATTGCATAAC 
TTGCGGAAAC TTTACCCTAT AGAAACAGCA GTTTCCAAGA TAAGTGAGAT AGGGAAGCTA 
ACTTCTCTTC AATATTTGCC TCAGTTTGAA GTCCGAAAGG ATGATCAACA CAAAATTGGA 
GAACTAAAGC ATATGACACA GCTTCATGGA TGCTTGCATA TTAAGAAGCT TGAAAATGTA 
GAGAGCAAGG AAGATGCTTG TGAAGCTGAG CTGGACAATA AGCCGTACCT TGATGAACTG 
ATTTTAGAAT GGTCTCGGGG AACAAGCAAT TCTCACCATG ATATAGAGGT GCTTGAAGGC 
CTTCAGCCAC GTCAAAAGCT TAAACAGCTG GAAATCAGAC ACTATGGTGG CACGACATCT 
CCCAGTTGGC TACGACCTCA AACATTAACC TGCTTAAGTA AACTCCACAT TGAAGAATGC 
AGCAAACTTA CCGAATTACC GTGCCTCCCT CCTTCCCTTA TGTCACTGGT GCTAGTAAAT 
GTGGGCCTGA ATTCACTTCC TAGATTGTGG GATGAATGCC AAGATTCAAC CAACGAAAGC 
AAAACTCAGC GAGGTGGTGA CAATAGCAGC AACAGGAGTT CTTCGCTACG TAAGTTACAC 
ATTAAGCGAT GCTTCAACCT GACGAGTCTT GAAGGGTGGC TGATGCCACA CTACTTACCA 
TCCCTTGAGG AATTCAGAGT CGAAAAGTGT AAAAATTTGA CTTTGCTTCC TCCTGACAGC 
TTCAAGGATT TTGTTTCTTT GAAGTCAATG GCAATAGACG TTTGTCCTAA GCTCACCTGC 
CGTACAGTAC TGATCTTACC CCCCTCAATT GAATTGGTTG TTCTCGATGA CTGTGGTAAT 
CTGGATCAGT CACTTCCTCG CTGCCTACAG AACCTTAAAT CTCTCATCAA TTTAACAATA 
ATTCGATGTC CACACGTAGC ATCCCTGCCG GAGCAAGTGA TGTGTCAACT CAAATCACTT 
GAGAAGCTGT TTATTTGGCG TTGTGAAGAG CTTAGTTCTT TAGGAGGATT AGGTGTTCTT 
GCATCTCTCA AATATATCAG AATTCGCAAC TGTCCCAAAC TTGCAGATTC TGAGCCACCG 
ATGTTTGACT TCACCGATGA AATACAGAGG GCGGAGATCG GTTTGCGCAT TGATAGGACG 
ACCCTTCTGA AGCTGCAATC TTTAAGGAAT CTGGTACCCT TCATCAGAGA ACTCGAAATT 
TGGAGTCCTG ATGAACCAGA AATGTTTGTT GGAGAGGACA GAGAACTGTT GCTGTTGTTT 
AAGTCACTTA AACATCTACA GTTTTCATTT TGTAAAGAAC TTAATTCGCT ACCAACTGAG 
TTGCATAGAT TGACTTCGCT TCAAAAGCTA TCCATCCACT ACTGCTCTAA GATCCACTTC 
TTGCCAGAGA ATGGCCTACC AGCCTCATTG ACGAAGTTAG ACTTCCACGG TTGCAATAAA 
GGAGTTTACG CTACAGCTGG AGATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020091272.1
CDS	25..2994
Translation	MGSLVDEPIVYGRDEEREKVIKLLGVPCGQSDIENDNESVSAPAKRRKKENVSVLSIYGIGGVGKTTLAQVVYNDERVVKHFDLKIWVCVSDNFDVNSLSKEIIDNAGKGYEADRMNLSSLQATLKEIVMAHRFLLVLDDVWNENSREWQKFYAPLSHGQVGSMILVTTRTLKVANIAGTMDPIFLEGLSGDVYWDFFKSCAFRCENPEDYPGLEKIGIDIAAKLKGSPLAAKTLGGLLNEDMDAGHWTTILNSELWELPQKEGDILPALRLSYLYLLPHLKRCFSFCSIFPKDYEFNKSDLTSIWVAEGFVIPQGNMLLEDIATMYLRDLTSRCFLQQSHRDPLNYVMHDLIHDLAQFVSVDECSRIDKGRGQIRIPKTVRHLSVCCTSIEEKKLMELASYDKLRSIIILSKFRRGLDSIIDCWFRRLANIRCLKLSFCKITKLPESISNLKHLRYLDVSGTSIRTLPTSLCHLNNLQVLNVMKCPLECFPQGFTGLHNLRKLYPIETAVSKISEIGKLTSLQYLPQFEVRKDDQHKIGELKHMTQLHGCLHIKKLENVESKEDACEAELDNKPYLDELILEWSRGTSNSHHDIEVLEGLQPRQKLKQLEIRHYGGTTSPSWLRPQTLTCLSKLHIEECSKLTELPCLPPSLMSLVLVNVGLNSLPRLWDECQDSTNESKTQRGGDNSSNRSSSLRKLHIKRCFNLTSLEGWLMPHYLPSLEEFRVEKCKNLTLLPPDSFKDFVSLKSMAIDVCPKLTCRTVLILPPSIELVVLDDCGNLDQSLPRCLQNLKSLINLTIIRCPHVASLPEQVMCQLKSLEKLFIWRCEELSSLGGLGVLASLKYIRIRNCPKLADSEPPMFDFTDEIQRAEIGLRIDRTTLLKLQSLRNLVPFIRELEIWSPDEPEMFVGEDRELLLLFKSLKHLQFSFCKELNSLPTELHRLTSLQKLSIHYCSKIHFLPENGLPASLTKLDFHGCNKGVYATAGDV

Target ORF information:

RefSeq Version	XM_020235683.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus putative disease resistance protein RGA3 (LOC109712220), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020235683.1

ORF Insert Sequence:

ATGGGCTCCT TAGTCGACGA ACCAATAGTA TATGGTCGAG ACGAGGAACG GGAGAAGGTG 
ATCAAGTTGT TGGGAGTTCC ATGCGGGCAG AGTGACATTG AAAACGACAA TGAAAGTGTA 
TCTGCCCCTG CTAAGAGAAG AAAGAAAGAA AATGTGTCTG TTCTATCCAT TTATGGCATC 
GGAGGGGTCG GCAAGACTAC TCTAGCACAG GTTGTCTACA ATGATGAGAG GGTGGTGAAG 
CACTTTGACC TGAAAATTTG GGTCTGTGTT TCTGACAACT TCGATGTGAA CAGCTTAAGT 
AAAGAGATCA TAGATAACGC TGGGAAGGGG TACGAGGCTG ATCGTATGAA TTTAAGTTCT 
CTTCAAGCGA CTCTAAAGGA GATAGTGATG GCACATCGCT TCTTGCTTGT CCTTGATGAT 
GTGTGGAATG AGAATAGTAG GGAGTGGCAA AAATTTTATG CGCCTTTGAG CCATGGGCAA 
GTAGGAAGTA TGATCCTGGT GACGACTCGG ACTTTGAAGG TTGCCAACAT AGCAGGAACA 
ATGGACCCCA TCTTCCTGGA GGGTTTATCA GGTGATGTTT ATTGGGATTT CTTCAAGTCA 
TGCGCATTTC GCTGTGAGAA CCCTGAAGAC TATCCGGGGT TAGAAAAAAT AGGAATAGAC 
ATTGCTGCTA AGTTGAAGGG ATCTCCGTTA GCTGCAAAAA CTTTAGGAGG CCTGTTGAAT 
GAGGACATGG ATGCCGGACA TTGGACTACG ATCTTGAACA GTGAATTATG GGAACTACCG 
CAGAAGGAAG GCGACATTCT TCCAGCCCTT CGATTGAGCT ATCTGTATCT TCTGCCGCAT 
CTGAAGCGAT GCTTCTCGTT TTGTTCGATA TTCCCAAAAG ATTATGAGTT CAATAAAAGT 
GATCTAACTA GTATTTGGGT AGCAGAAGGC TTTGTTATAC CTCAAGGAAA CATGCTACTA 
GAAGATATAG CGACCATGTA CTTGCGCGAC TTAACAAGCA GGTGCTTCCT TCAGCAATCT 
CATAGGGATC CTCTAAACTA TGTAATGCAT GATTTGATAC ACGATCTAGC GCAATTTGTT 
TCGGTAGACG AATGTTCAAG GATAGATAAA GGTAGAGGAC AAATAAGAAT CCCTAAAACT 
GTTCGACATT TGTCGGTATG CTGTACGAGT ATAGAAGAAA AGAAGTTAAT GGAGTTAGCG 
AGCTACGACA AACTGCGCTC GATTATAATA CTTTCTAAAT TTCGGCGTGG ATTAGATTCT 
ATAATTGATT GCTGGTTCCG CAGGCTCGCA AATATCCGTT GTTTAAAACT TAGCTTCTGT 
AAGATAACAA AGCTTCCTGA GAGTATTAGC AACTTAAAGC ATCTCCGATA CCTTGATGTA 
TCTGGTACAA GTATCAGAAC GTTACCCACG TCACTGTGTC ACCTCAATAA TCTGCAAGTG 
TTGAACGTAA TGAAGTGCCC GCTTGAGTGT TTTCCTCAAG GATTTACTGG ATTGCATAAC 
TTGCGGAAAC TTTACCCTAT AGAAACAGCA GTTTCCAAGA TAAGTGAGAT AGGGAAGCTA 
ACTTCTCTTC AATATTTGCC TCAGTTTGAA GTCCGAAAGG ATGATCAACA CAAAATTGGA 
GAACTAAAGC ATATGACACA GCTTCATGGA TGCTTGCATA TTAAGAAGCT TGAAAATGTA 
GAGAGCAAGG AAGATGCTTG TGAAGCTGAG CTGGACAATA AGCCGTACCT TGATGAACTG 
ATTTTAGAAT GGTCTCGGGG AACAAGCAAT TCTCACCATG ATATAGAGGT GCTTGAAGGC 
CTTCAGCCAC GTCAAAAGCT TAAACAGCTG GAAATCAGAC ACTATGGTGG CACGACATCT 
CCCAGTTGGC TACGACCTCA AACATTAACC TGCTTAAGTA AACTCCACAT TGAAGAATGC 
AGCAAACTTA CCGAATTACC GTGCCTCCCT CCTTCCCTTA TGTCACTGGT GCTAGTAAAT 
GTGGGCCTGA ATTCACTTCC TAGATTGTGG GATGAATGCC AAGATTCAAC CAACGAAAGC 
AAAACTCAGC GAGGTGGTGA CAATAGCAGC AACAGGAGTT CTTCGCTACG TAAGTTACAC 
ATTAAGCGAT GCTTCAACCT GACGAGTCTT GAAGGGTGGC TGATGCCACA CTACTTACCA 
TCCCTTGAGG AATTCAGAGT CGAAAAGTGT AAAAATTTGA CTTTGCTTCC TCCTGACAGC 
TTCAAGGATT TTGTTTCTTT GAAGTCAATG GCAATAGACG TTTGTCCTAA GCTCACCTGC 
CGTACAGTAC TGATCTTACC CCCCTCAATT GAATTGGTTG TTCTCGATGA CTGTGGTAAT 
CTGGATCAGT CACTTCCTCG CTGCCTACAG AACCTTAAAT CTCTCATCAA TTTAACAATA 
ATTCGATGTC CACACGTAGC ATCCCTGCCG GAGCAAGTGA TGTGTCAACT CAAATCACTT 
GAGAAGCTGT TTATTTGGCG TTGTGAAGAG CTTAGTTCTT TAGGAGGATT AGGTGTTCTT 
GCATCTCTCA AATATATCAG AATTCGCAAC TGTCCCAAAC TTGCAGATTC TGAGCCACCG 
ATGTTTGACT TCACCGATGA AATACAGAGG GCGGAGATCG GTTTGCGCAT TGATAGGACG 
ACCCTTCTGA AGCTGCAATC TTTAAGGAAT CTGGTACCCT TCATCAGAGA ACTCGAAATT 
TGGAGTCCTG ATGAACCAGA AATGTTTGTT GGAGAGGACA GAGAACTGTT GCTGTTGTTT 
AAGTCACTTA AACATCTACA GTTTTCATTT TGTAAAGAAC TTAATTCGCT ACCAACTGAG 
TTGCATAGAT TGACTTCGCT TCAAAAGCTA TCCATCCACT ACTGCTCTAA GATCCACTTC 
TTGCCAGAGA ATGGCCTACC AGCCTCATTG ACGAAGTTAG ACTTCCACGG TTGCAATAAA 
GGAGTTTACG CTACAGCTGG AGATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109712220 ( XM_020235675.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020091264.1 Ananas comosus putative disease resistance protein RGA3 (LOC109712220), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn58784
Clone ID Related Accession (Same CDS sequence)	XM_020235683.1 , XM_020235675.1
Accession Version	XM_020235675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	putative disease resistance protein RGA3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033621.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCCT TAGTCGACGA ACCAATAGTA TATGGTCGAG ACGAGGAACG GGAGAAGGTG 
ATCAAGTTGT TGGGAGTTCC ATGCGGGCAG AGTGACATTG AAAACGACAA TGAAAGTGTA 
TCTGCCCCTG CTAAGAGAAG AAAGAAAGAA AATGTGTCTG TTCTATCCAT TTATGGCATC 
GGAGGGGTCG GCAAGACTAC TCTAGCACAG GTTGTCTACA ATGATGAGAG GGTGGTGAAG 
CACTTTGACC TGAAAATTTG GGTCTGTGTT TCTGACAACT TCGATGTGAA CAGCTTAAGT 
AAAGAGATCA TAGATAACGC TGGGAAGGGG TACGAGGCTG ATCGTATGAA TTTAAGTTCT 
CTTCAAGCGA CTCTAAAGGA GATAGTGATG GCACATCGCT TCTTGCTTGT CCTTGATGAT 
GTGTGGAATG AGAATAGTAG GGAGTGGCAA AAATTTTATG CGCCTTTGAG CCATGGGCAA 
GTAGGAAGTA TGATCCTGGT GACGACTCGG ACTTTGAAGG TTGCCAACAT AGCAGGAACA 
ATGGACCCCA TCTTCCTGGA GGGTTTATCA GGTGATGTTT ATTGGGATTT CTTCAAGTCA 
TGCGCATTTC GCTGTGAGAA CCCTGAAGAC TATCCGGGGT TAGAAAAAAT AGGAATAGAC 
ATTGCTGCTA AGTTGAAGGG ATCTCCGTTA GCTGCAAAAA CTTTAGGAGG CCTGTTGAAT 
GAGGACATGG ATGCCGGACA TTGGACTACG ATCTTGAACA GTGAATTATG GGAACTACCG 
CAGAAGGAAG GCGACATTCT TCCAGCCCTT CGATTGAGCT ATCTGTATCT TCTGCCGCAT 
CTGAAGCGAT GCTTCTCGTT TTGTTCGATA TTCCCAAAAG ATTATGAGTT CAATAAAAGT 
GATCTAACTA GTATTTGGGT AGCAGAAGGC TTTGTTATAC CTCAAGGAAA CATGCTACTA 
GAAGATATAG CGACCATGTA CTTGCGCGAC TTAACAAGCA GGTGCTTCCT TCAGCAATCT 
CATAGGGATC CTCTAAACTA TGTAATGCAT GATTTGATAC ACGATCTAGC GCAATTTGTT 
TCGGTAGACG AATGTTCAAG GATAGATAAA GGTAGAGGAC AAATAAGAAT CCCTAAAACT 
GTTCGACATT TGTCGGTATG CTGTACGAGT ATAGAAGAAA AGAAGTTAAT GGAGTTAGCG 
AGCTACGACA AACTGCGCTC GATTATAATA CTTTCTAAAT TTCGGCGTGG ATTAGATTCT 
ATAATTGATT GCTGGTTCCG CAGGCTCGCA AATATCCGTT GTTTAAAACT TAGCTTCTGT 
AAGATAACAA AGCTTCCTGA GAGTATTAGC AACTTAAAGC ATCTCCGATA CCTTGATGTA 
TCTGGTACAA GTATCAGAAC GTTACCCACG TCACTGTGTC ACCTCAATAA TCTGCAAGTG 
TTGAACGTAA TGAAGTGCCC GCTTGAGTGT TTTCCTCAAG GATTTACTGG ATTGCATAAC 
TTGCGGAAAC TTTACCCTAT AGAAACAGCA GTTTCCAAGA TAAGTGAGAT AGGGAAGCTA 
ACTTCTCTTC AATATTTGCC TCAGTTTGAA GTCCGAAAGG ATGATCAACA CAAAATTGGA 
GAACTAAAGC ATATGACACA GCTTCATGGA TGCTTGCATA TTAAGAAGCT TGAAAATGTA 
GAGAGCAAGG AAGATGCTTG TGAAGCTGAG CTGGACAATA AGCCGTACCT TGATGAACTG 
ATTTTAGAAT GGTCTCGGGG AACAAGCAAT TCTCACCATG ATATAGAGGT GCTTGAAGGC 
CTTCAGCCAC GTCAAAAGCT TAAACAGCTG GAAATCAGAC ACTATGGTGG CACGACATCT 
CCCAGTTGGC TACGACCTCA AACATTAACC TGCTTAAGTA AACTCCACAT TGAAGAATGC 
AGCAAACTTA CCGAATTACC GTGCCTCCCT CCTTCCCTTA TGTCACTGGT GCTAGTAAAT 
GTGGGCCTGA ATTCACTTCC TAGATTGTGG GATGAATGCC AAGATTCAAC CAACGAAAGC 
AAAACTCAGC GAGGTGGTGA CAATAGCAGC AACAGGAGTT CTTCGCTACG TAAGTTACAC 
ATTAAGCGAT GCTTCAACCT GACGAGTCTT GAAGGGTGGC TGATGCCACA CTACTTACCA 
TCCCTTGAGG AATTCAGAGT CGAAAAGTGT AAAAATTTGA CTTTGCTTCC TCCTGACAGC 
TTCAAGGATT TTGTTTCTTT GAAGTCAATG GCAATAGACG TTTGTCCTAA GCTCACCTGC 
CGTACAGTAC TGATCTTACC CCCCTCAATT GAATTGGTTG TTCTCGATGA CTGTGGTAAT 
CTGGATCAGT CACTTCCTCG CTGCCTACAG AACCTTAAAT CTCTCATCAA TTTAACAATA 
ATTCGATGTC CACACGTAGC ATCCCTGCCG GAGCAAGTGA TGTGTCAACT CAAATCACTT 
GAGAAGCTGT TTATTTGGCG TTGTGAAGAG CTTAGTTCTT TAGGAGGATT AGGTGTTCTT 
GCATCTCTCA AATATATCAG AATTCGCAAC TGTCCCAAAC TTGCAGATTC TGAGCCACCG 
ATGTTTGACT TCACCGATGA AATACAGAGG GCGGAGATCG GTTTGCGCAT TGATAGGACG 
ACCCTTCTGA AGCTGCAATC TTTAAGGAAT CTGGTACCCT TCATCAGAGA ACTCGAAATT 
TGGAGTCCTG ATGAACCAGA AATGTTTGTT GGAGAGGACA GAGAACTGTT GCTGTTGTTT 
AAGTCACTTA AACATCTACA GTTTTCATTT TGTAAAGAAC TTAATTCGCT ACCAACTGAG 
TTGCATAGAT TGACTTCGCT TCAAAAGCTA TCCATCCACT ACTGCTCTAA GATCCACTTC 
TTGCCAGAGA ATGGCCTACC AGCCTCATTG ACGAAGTTAG ACTTCCACGG TTGCAATAAA 
GGAGTTTACG CTACAGCTGG AGATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020091264.1
CDS	25..2994
Translation	MGSLVDEPIVYGRDEEREKVIKLLGVPCGQSDIENDNESVSAPAKRRKKENVSVLSIYGIGGVGKTTLAQVVYNDERVVKHFDLKIWVCVSDNFDVNSLSKEIIDNAGKGYEADRMNLSSLQATLKEIVMAHRFLLVLDDVWNENSREWQKFYAPLSHGQVGSMILVTTRTLKVANIAGTMDPIFLEGLSGDVYWDFFKSCAFRCENPEDYPGLEKIGIDIAAKLKGSPLAAKTLGGLLNEDMDAGHWTTILNSELWELPQKEGDILPALRLSYLYLLPHLKRCFSFCSIFPKDYEFNKSDLTSIWVAEGFVIPQGNMLLEDIATMYLRDLTSRCFLQQSHRDPLNYVMHDLIHDLAQFVSVDECSRIDKGRGQIRIPKTVRHLSVCCTSIEEKKLMELASYDKLRSIIILSKFRRGLDSIIDCWFRRLANIRCLKLSFCKITKLPESISNLKHLRYLDVSGTSIRTLPTSLCHLNNLQVLNVMKCPLECFPQGFTGLHNLRKLYPIETAVSKISEIGKLTSLQYLPQFEVRKDDQHKIGELKHMTQLHGCLHIKKLENVESKEDACEAELDNKPYLDELILEWSRGTSNSHHDIEVLEGLQPRQKLKQLEIRHYGGTTSPSWLRPQTLTCLSKLHIEECSKLTELPCLPPSLMSLVLVNVGLNSLPRLWDECQDSTNESKTQRGGDNSSNRSSSLRKLHIKRCFNLTSLEGWLMPHYLPSLEEFRVEKCKNLTLLPPDSFKDFVSLKSMAIDVCPKLTCRTVLILPPSIELVVLDDCGNLDQSLPRCLQNLKSLINLTIIRCPHVASLPEQVMCQLKSLEKLFIWRCEELSSLGGLGVLASLKYIRIRNCPKLADSEPPMFDFTDEIQRAEIGLRIDRTTLLKLQSLRNLVPFIRELEIWSPDEPEMFVGEDRELLLLFKSLKHLQFSFCKELNSLPTELHRLTSLQKLSIHYCSKIHFLPENGLPASLTKLDFHGCNKGVYATAGDV

Target ORF information:

RefSeq Version	XM_020235675.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus putative disease resistance protein RGA3 (LOC109712220), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020235675.1

ORF Insert Sequence:

ATGGGCTCCT TAGTCGACGA ACCAATAGTA TATGGTCGAG ACGAGGAACG GGAGAAGGTG 
ATCAAGTTGT TGGGAGTTCC ATGCGGGCAG AGTGACATTG AAAACGACAA TGAAAGTGTA 
TCTGCCCCTG CTAAGAGAAG AAAGAAAGAA AATGTGTCTG TTCTATCCAT TTATGGCATC 
GGAGGGGTCG GCAAGACTAC TCTAGCACAG GTTGTCTACA ATGATGAGAG GGTGGTGAAG 
CACTTTGACC TGAAAATTTG GGTCTGTGTT TCTGACAACT TCGATGTGAA CAGCTTAAGT 
AAAGAGATCA TAGATAACGC TGGGAAGGGG TACGAGGCTG ATCGTATGAA TTTAAGTTCT 
CTTCAAGCGA CTCTAAAGGA GATAGTGATG GCACATCGCT TCTTGCTTGT CCTTGATGAT 
GTGTGGAATG AGAATAGTAG GGAGTGGCAA AAATTTTATG CGCCTTTGAG CCATGGGCAA 
GTAGGAAGTA TGATCCTGGT GACGACTCGG ACTTTGAAGG TTGCCAACAT AGCAGGAACA 
ATGGACCCCA TCTTCCTGGA GGGTTTATCA GGTGATGTTT ATTGGGATTT CTTCAAGTCA 
TGCGCATTTC GCTGTGAGAA CCCTGAAGAC TATCCGGGGT TAGAAAAAAT AGGAATAGAC 
ATTGCTGCTA AGTTGAAGGG ATCTCCGTTA GCTGCAAAAA CTTTAGGAGG CCTGTTGAAT 
GAGGACATGG ATGCCGGACA TTGGACTACG ATCTTGAACA GTGAATTATG GGAACTACCG 
CAGAAGGAAG GCGACATTCT TCCAGCCCTT CGATTGAGCT ATCTGTATCT TCTGCCGCAT 
CTGAAGCGAT GCTTCTCGTT TTGTTCGATA TTCCCAAAAG ATTATGAGTT CAATAAAAGT 
GATCTAACTA GTATTTGGGT AGCAGAAGGC TTTGTTATAC CTCAAGGAAA CATGCTACTA 
GAAGATATAG CGACCATGTA CTTGCGCGAC TTAACAAGCA GGTGCTTCCT TCAGCAATCT 
CATAGGGATC CTCTAAACTA TGTAATGCAT GATTTGATAC ACGATCTAGC GCAATTTGTT 
TCGGTAGACG AATGTTCAAG GATAGATAAA GGTAGAGGAC AAATAAGAAT CCCTAAAACT 
GTTCGACATT TGTCGGTATG CTGTACGAGT ATAGAAGAAA AGAAGTTAAT GGAGTTAGCG 
AGCTACGACA AACTGCGCTC GATTATAATA CTTTCTAAAT TTCGGCGTGG ATTAGATTCT 
ATAATTGATT GCTGGTTCCG CAGGCTCGCA AATATCCGTT GTTTAAAACT TAGCTTCTGT 
AAGATAACAA AGCTTCCTGA GAGTATTAGC AACTTAAAGC ATCTCCGATA CCTTGATGTA 
TCTGGTACAA GTATCAGAAC GTTACCCACG TCACTGTGTC ACCTCAATAA TCTGCAAGTG 
TTGAACGTAA TGAAGTGCCC GCTTGAGTGT TTTCCTCAAG GATTTACTGG ATTGCATAAC 
TTGCGGAAAC TTTACCCTAT AGAAACAGCA GTTTCCAAGA TAAGTGAGAT AGGGAAGCTA 
ACTTCTCTTC AATATTTGCC TCAGTTTGAA GTCCGAAAGG ATGATCAACA CAAAATTGGA 
GAACTAAAGC ATATGACACA GCTTCATGGA TGCTTGCATA TTAAGAAGCT TGAAAATGTA 
GAGAGCAAGG AAGATGCTTG TGAAGCTGAG CTGGACAATA AGCCGTACCT TGATGAACTG 
ATTTTAGAAT GGTCTCGGGG AACAAGCAAT TCTCACCATG ATATAGAGGT GCTTGAAGGC 
CTTCAGCCAC GTCAAAAGCT TAAACAGCTG GAAATCAGAC ACTATGGTGG CACGACATCT 
CCCAGTTGGC TACGACCTCA AACATTAACC TGCTTAAGTA AACTCCACAT TGAAGAATGC 
AGCAAACTTA CCGAATTACC GTGCCTCCCT CCTTCCCTTA TGTCACTGGT GCTAGTAAAT 
GTGGGCCTGA ATTCACTTCC TAGATTGTGG GATGAATGCC AAGATTCAAC CAACGAAAGC 
AAAACTCAGC GAGGTGGTGA CAATAGCAGC AACAGGAGTT CTTCGCTACG TAAGTTACAC 
ATTAAGCGAT GCTTCAACCT GACGAGTCTT GAAGGGTGGC TGATGCCACA CTACTTACCA 
TCCCTTGAGG AATTCAGAGT CGAAAAGTGT AAAAATTTGA CTTTGCTTCC TCCTGACAGC 
TTCAAGGATT TTGTTTCTTT GAAGTCAATG GCAATAGACG TTTGTCCTAA GCTCACCTGC 
CGTACAGTAC TGATCTTACC CCCCTCAATT GAATTGGTTG TTCTCGATGA CTGTGGTAAT 
CTGGATCAGT CACTTCCTCG CTGCCTACAG AACCTTAAAT CTCTCATCAA TTTAACAATA 
ATTCGATGTC CACACGTAGC ATCCCTGCCG GAGCAAGTGA TGTGTCAACT CAAATCACTT 
GAGAAGCTGT TTATTTGGCG TTGTGAAGAG CTTAGTTCTT TAGGAGGATT AGGTGTTCTT 
GCATCTCTCA AATATATCAG AATTCGCAAC TGTCCCAAAC TTGCAGATTC TGAGCCACCG 
ATGTTTGACT TCACCGATGA AATACAGAGG GCGGAGATCG GTTTGCGCAT TGATAGGACG 
ACCCTTCTGA AGCTGCAATC TTTAAGGAAT CTGGTACCCT TCATCAGAGA ACTCGAAATT 
TGGAGTCCTG ATGAACCAGA AATGTTTGTT GGAGAGGACA GAGAACTGTT GCTGTTGTTT 
AAGTCACTTA AACATCTACA GTTTTCATTT TGTAAAGAAC TTAATTCGCT ACCAACTGAG 
TTGCATAGAT TGACTTCGCT TCAAAAGCTA TCCATCCACT ACTGCTCTAA GATCCACTTC 
TTGCCAGAGA ATGGCCTACC AGCCTCATTG ACGAAGTTAG ACTTCCACGG TTGCAATAAA 
GGAGTTTACG CTACAGCTGG AGATGTATAA