LOC105416331 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105416331
Entrez Gene ID	105416331
Full Name	prisilkin-39-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029837774.1	XP_029693634.1	fibroin heavy chain-like isoform X3
XM_029837763.1	XP_029693623.1	filaggrin-2-like isoform X1
XM_011603034.2	XP_011601336.2	spidroin-1-like isoform X4
XM_011603034.1	XP_011601336.1	prisilkin-39-like
XM_029837769.1	XP_029693629.1	filaggrin-2-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC105416331 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105416331 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo32504	XM_011603034.1 Latest version!	Takifugu rubripes prisilkin-39-like (LOC105416331), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OTo41607	XM_011603034.2 Latest version!	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo41604	XM_029837763.1 Latest version!	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo41605	XM_029837769.1 Latest version!	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo41606	XM_029837774.1 Latest version!	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105416331 ( XM_011603034.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601336.1 Takifugu rubripes prisilkin-39-like (LOC105416331), mRNA.

Price & Availability↑

CloneID	OTo32504
Clone ID Related Accession (Same CDS sequence)	XM_011603034.1
Accession Version	XM_011603034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	prisilkin-39-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070901.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGCA TGGATGAAGT GAATGGACGG ACTCTTAAAG GTGGAGTTCT TACTCCAGAA 
CAGCCGACAG CAGTACCAAA TATGAGTGCC ATCCCTCAAC AGGGAACAAC TGGCAGACCA 
GCACCAGTGA AGGGAGAAGC TTCATCCTTA AAGGGACAGG GAGCTAACCA ACAGAGACCT 
GACTGTGGAG CACCGCTTGG ACAATGGATG AAAATACCTA GGCCTGGTTA TGATTCAGGT 
GTTGGAGCAT CATCCAGCAT AAACACAAAA GGGCTTGGAC ATCCCTATGC TCAGCAACCA 
GGTTATGGAC AAGGAGGCTA CCTTGGAGCA GGATATGGAA AAGGAGGAAA TGCAGGTTTT 
GGTTCCAAAT CAGGATTCAG AAATGGCTAT AATGCTGGTG TGCAGCCCGA CTACGCCAGT 
CTTGTACCTG CAGCTAACAG CAAATCAGGA ATGAGCGGGG TGCCATTTGG TGGTCTGTCT 
GCAGGAATGG GCGCAGAGAA AATGAACACA AAATATGGCA TTGGCGGGTT ACAGTTTGAT 
GGACAGCAGT TCAATACTGG GATTAATGGA GCAGGAAGTT ATGGTTATGG TCAAAGCCCA 
CATGGACCCA CTGGCCAAGG CAAAACATCA GGAAAATATG GTGGTGTTGG TGCCGGTGCC 
AGCGTGGGGG GCAGTCCTGG AAAATACGGA TTCGGTGGAT TTCACAACAG TCAACAGGTT 
TTCGGTCTTG GACCTACTGC AAATGGAAAC TATGGTTATG GAAGAATGTC CTATGAACCT 
GAACCAGCTG TAATGGGCCC TGAAGCTCCT GGAAATTATG GTCTGACTGC ATCACAATAT 
CAGCCCGAAC CCCTTGGACT TGGACACAAT GGGCAATTAA GGAGTAATTA TGATGCTGTG 
CCTTATGAAA GCCTGCCTGT TGAGACGGAC CCGCTACAAA ATCCTTATGT GAACGGAGAC 
GTACTGACCC CTGTGGGTGT GAGTGTTGAA GGGGATCACT ATGAGAACAT GGGCTATATC 
AATGGACAAG TGCAGCCAGA AGAAGCTCCC ACTGCCATCC CCTCCCTGGC CTACCCTGCT 
CTTCCAGCAC ATCTGCCTGC AGAGTCCTCC CTCACTCCTG ATGTGGGGCT GCCAGCAGAG 
GTTGAGGACT TGCCCGACCC TGCAGGTACT GCCAGCATCC CCCTGGACTC TGAGCCTGCC 
ACAGAAACAC CAGGCCTGCC TCAGTCGCAC GAGCAACCCG AGGATGATCC GCAGCAGCTG 
CCGCGACAGA TTCACATTCA GCAGCACCTC AAACTGCATT TCCACAACCA AGGAACAAAT 
GATGGCAACA AATATGATTT GAATGGTTTC TTTGGAAACA GTGGATATCA AAGTGAGAAC 
GGACATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601336.1
CDS	20..1411
Translation	MKGMDEVNGRTLKGGVLTPEQPTAVPNMSAIPQQGTTGRPAPVKGEASSLKGQGANQQRPDCGAPLGQWMKIPRPGYDSGVGASSSINTKGLGHPYAQQPGYGQGGYLGAGYGKGGNAGFGSKSGFRNGYNAGVQPDYASLVPAANSKSGMSGVPFGGLSAGMGAEKMNTKYGIGGLQFDGQQFNTGINGAGSYGYGQSPHGPTGQGKTSGKYGGVGAGASVGGSPGKYGFGGFHNSQQVFGLGPTANGNYGYGRMSYEPEPAVMGPEAPGNYGLTASQYQPEPLGLGHNGQLRSNYDAVPYESLPVETDPLQNPYVNGDVLTPVGVSVEGDHYENMGYINGQVQPEEAPTAIPSLAYPALPAHLPAESSLTPDVGLPAEVEDLPDPAGTASIPLDSEPATETPGLPQSHEQPEDDPQQLPRQIHIQQHLKLHFHNQGTNDGNKYDLNGFFGNSGYQSENGHQ

Target ORF information:

RefSeq Version	XM_011603034.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes prisilkin-39-like (LOC105416331), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603034.1

ORF Insert Sequence:

ATGAAGGGCA TGGATGAAGT GAATGGACGG ACTCTTAAAG GTGGAGTTCT TACTCCAGAA 
CAGCCGACAG CAGTACCAAA TATGAGTGCC ATCCCTCAAC AGGGAACAAC TGGCAGACCA 
GCACCAGTGA AGGGAGAAGC TTCATCCTTA AAGGGACAGG GAGCTAACCA ACAGAGACCT 
GACTGTGGAG CACCGCTTGG ACAATGGATG AAAATACCTA GGCCTGGTTA TGATTCAGGT 
GTTGGAGCAT CATCCAGCAT AAACACAAAA GGGCTTGGAC ATCCCTATGC TCAGCAACCA 
GGTTATGGAC AAGGAGGCTA CCTTGGAGCA GGATATGGAA AAGGAGGAAA TGCAGGTTTT 
GGTTCCAAAT CAGGATTCAG AAATGGCTAT AATGCTGGTG TGCAGCCCGA CTACGCCAGT 
CTTGTACCTG CAGCTAACAG CAAATCAGGA ATGAGCGGGG TGCCATTTGG TGGTCTGTCT 
GCAGGAATGG GCGCAGAGAA AATGAACACA AAATATGGCA TTGGCGGGTT ACAGTTTGAT 
GGACAGCAGT TCAATACTGG GATTAATGGA GCAGGAAGTT ATGGTTATGG TCAAAGCCCA 
CATGGACCCA CTGGCCAAGG CAAAACATCA GGAAAATATG GTGGTGTTGG TGCCGGTGCC 
AGCGTGGGGG GCAGTCCTGG AAAATACGGA TTCGGTGGAT TTCACAACAG TCAACAGGTT 
TTCGGTCTTG GACCTACTGC AAATGGAAAC TATGGTTATG GAAGAATGTC CTATGAACCT 
GAACCAGCTG TAATGGGCCC TGAAGCTCCT GGAAATTATG GTCTGACTGC ATCACAATAT 
CAGCCCGAAC CCCTTGGACT TGGACACAAT GGGCAATTAA GGAGTAATTA TGATGCTGTG 
CCTTATGAAA GCCTGCCTGT TGAGACGGAC CCGCTACAAA ATCCTTATGT GAACGGAGAC 
GTACTGACCC CTGTGGGTGT GAGTGTTGAA GGGGATCACT ATGAGAACAT GGGCTATATC 
AATGGACAAG TGCAGCCAGA AGAAGCTCCC ACTGCCATCC CCTCCCTGGC CTACCCTGCT 
CTTCCAGCAC ATCTGCCTGC AGAGTCCTCC CTCACTCCTG ATGTGGGGCT GCCAGCAGAG 
GTTGAGGACT TGCCCGACCC TGCAGGTACT GCCAGCATCC CCCTGGACTC TGAGCCTGCC 
ACAGAAACAC CAGGCCTGCC TCAGTCGCAC GAGCAACCCG AGGATGATCC GCAGCAGCTG 
CCGCGACAGA TTCACATTCA GCAGCACCTC AAACTGCATT TCCACAACCA AGGAACAAAT 
GATGGCAACA AATATGATTT GAATGGTTTC TTTGGAAACA GTGGATATCA AAGTGAGAAC 
GGACATCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105416331 ( XM_011603034.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601336.2 Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo41607
Clone ID Related Accession (Same CDS sequence)	XM_011603034.2
Accession Version	XM_011603034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	spidroin-1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011603034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GTGAAGGGAG AAGCTTCATC CTTAAAGGGA CAGGGAGCTA ACCAACAGAG ACCTGACTGT 
GGAGCACCGC TTGGACAATG GATGAAAATA CCTAGGCCTG GTTATGTTTC AGGTGTTGGA 
GCATCATCCA GCATAAACAC AAAAGGGCTT GGACATCCCT ATGCTCAGCA ACCAGGTTAT 
GGACAAGGAG GCTACCTTGG AGCAGGATAT GGAAAAGGAG GAAATGCAGG TTTTGGTTCC 
AAATCAGGAT TCAGAAATGG CTATAATGCT GGTGTGCAGC CCGACTACGC CAGTCTTGTA 
CCTGCAGCTA ACAGCAAATC AGGAATGAGC GGGGTGCCAT TTGGTGGTCT GTCTGCAGGA 
ATGGGCGCAG AGAAAATGAA CACAAAATAT GGCATTGGCG GGTTACAGTT TGATGGACAG 
CAGTTCAATA CTGGGATTAA TGGAGCAGGA AGTTATGGTT ATGGTCAAAG CCCACATGGA 
CCCACTGGCC AAGGCAAAAC ATCAGGAAAA TATGGTGGTG TTGGTGCCGG TGCCAGCGTG 
GGGGGCAGTC CTGGAAAATA CGGATTCGGT GGATTTCACA ACAGTCAACA GGTTTTCGGT 
CTTGGACCTA CTGCAAATGG AAACTATGGT TATGGAAGAA TGTCCTATGA ACCTGAACCA 
GCTGTAATGG GCCCTGAAGC TCCTGGAAAT TATGGTCTGA CTGCATCACA ATATCAGCCC 
GAACCCCTTG GACTTGGACA CAATGGGCAA TTAAGGAGTA ATTATGATGC TGTGCCTTAT 
GAAAGCCTGC CTGTTGAGAC GGACCCGCTA CAAAATCCTT ATGTGAACGG AGACGTACTG 
ACCCCTGTGG GTGTGAGTGT TGAAGGGGAT CACTATGAGA ACATGGGCTA TATCAATGGA 
CAAGTGCAGC CAGAAGAAGC TCCCACTGCC ATCCCCTCCC TGGCCTACCC TGCTCTTCCA 
GCACATCTGC CTGCAGAGTC CTCCCTCACT CCTGATGTGG GGCTGCCAGC AGAGGTTGAG 
GACTTGCCCG ACCCTGCAGG TACTGCCAGC ATCCCCCTGG ACTCTGAGCC TGCCACAGAA 
ACACCAGGCC TGCCTCAGTC GCACGAGCAA CCCGAGGATG ATCCGCAGCA GCTGCCGCGA 
CAGATTCACA TTCAGCAGCA CCTCAAACTG CATTTCCACA ACCAAGGCAC AAATGATGGC 
AACAAATATG ATTTGAATGG TTTCTTTGGA AACAGTGGAT ATCAAAGTGA GAACGGACAT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601336.2
CDS	337..3222
Translation	MLGRLFLQSVVMVWLAHTAHTWGYGATTGVSNGPANGDTFQKGGGLGSMLMPSKGVGQGVGAQNGYGGYPTKGVGFGAAGAGAATKGWMKGYGAGAARGQPQGGDAAVLSKGNGAKSNGNGAAGVHNGPETKGKGYGSNGFKGYGGEPIRGYASAHGSAGLGLGHQFGNERTKGPTQEYGGSALRSQQGYGSRALPNGYGQNVNRYGQNVKGYGQKTKGYGQNPNGNAQNFDGYGPKPNGYGQTPSRYGQKPNGYGQTPNGNGQKPKGFGQIPNQNGQNVNGYGQKPNGYGQKPNGYGQKLKGYGQTPNRNGQKPNGYGQTPNGYGQKPKGYGQTPNRNGQKPNGYGQTPNGHRQKPNGYGQTSNGYGQKLKGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGHRQKPNGYGQTSNGNGQNLNGHEAAVFKDYGAKSKGHGVSNGAGTGVSGGTNSHNTKGLGTASKSEGNGQTPNAKNMKGMDEVNGRTLKGGVLTPEQPTAVPNMSAIPQQGTTGRPAPVKGEASSLKGQGANQQRPDCGAPLGQWMKIPRPGYVSGVGASSSINTKGLGHPYAQQPGYGQGGYLGAGYGKGGNAGFGSKSGFRNGYNAGVQPDYASLVPAANSKSGMSGVPFGGLSAGMGAEKMNTKYGIGGLQFDGQQFNTGINGAGSYGYGQSPHGPTGQGKTSGKYGGVGAGASVGGSPGKYGFGGFHNSQQVFGLGPTANGNYGYGRMSYEPEPAVMGPEAPGNYGLTASQYQPEPLGLGHNGQLRSNYDAVPYESLPVETDPLQNPYVNGDVLTPVGVSVEGDHYENMGYINGQVQPEEAPTAIPSLAYPALPAHLPAESSLTPDVGLPAEVEDLPDPAGTASIPLDSEPATETPGLPQSHEQPEDDPQQLPRQIHIQQHLKLHFHNQGTNDGNKYDLNGFFGNSGYQSENGHQ

Target ORF information:

RefSeq Version	XM_011603034.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603034.2

ORF Insert Sequence:

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GTGAAGGGAG AAGCTTCATC CTTAAAGGGA CAGGGAGCTA ACCAACAGAG ACCTGACTGT 
GGAGCACCGC TTGGACAATG GATGAAAATA CCTAGGCCTG GTTATGTTTC AGGTGTTGGA 
GCATCATCCA GCATAAACAC AAAAGGGCTT GGACATCCCT ATGCTCAGCA ACCAGGTTAT 
GGACAAGGAG GCTACCTTGG AGCAGGATAT GGAAAAGGAG GAAATGCAGG TTTTGGTTCC 
AAATCAGGAT TCAGAAATGG CTATAATGCT GGTGTGCAGC CCGACTACGC CAGTCTTGTA 
CCTGCAGCTA ACAGCAAATC AGGAATGAGC GGGGTGCCAT TTGGTGGTCT GTCTGCAGGA 
ATGGGCGCAG AGAAAATGAA CACAAAATAT GGCATTGGCG GGTTACAGTT TGATGGACAG 
CAGTTCAATA CTGGGATTAA TGGAGCAGGA AGTTATGGTT ATGGTCAAAG CCCACATGGA 
CCCACTGGCC AAGGCAAAAC ATCAGGAAAA TATGGTGGTG TTGGTGCCGG TGCCAGCGTG 
GGGGGCAGTC CTGGAAAATA CGGATTCGGT GGATTTCACA ACAGTCAACA GGTTTTCGGT 
CTTGGACCTA CTGCAAATGG AAACTATGGT TATGGAAGAA TGTCCTATGA ACCTGAACCA 
GCTGTAATGG GCCCTGAAGC TCCTGGAAAT TATGGTCTGA CTGCATCACA ATATCAGCCC 
GAACCCCTTG GACTTGGACA CAATGGGCAA TTAAGGAGTA ATTATGATGC TGTGCCTTAT 
GAAAGCCTGC CTGTTGAGAC GGACCCGCTA CAAAATCCTT ATGTGAACGG AGACGTACTG 
ACCCCTGTGG GTGTGAGTGT TGAAGGGGAT CACTATGAGA ACATGGGCTA TATCAATGGA 
CAAGTGCAGC CAGAAGAAGC TCCCACTGCC ATCCCCTCCC TGGCCTACCC TGCTCTTCCA 
GCACATCTGC CTGCAGAGTC CTCCCTCACT CCTGATGTGG GGCTGCCAGC AGAGGTTGAG 
GACTTGCCCG ACCCTGCAGG TACTGCCAGC ATCCCCCTGG ACTCTGAGCC TGCCACAGAA 
ACACCAGGCC TGCCTCAGTC GCACGAGCAA CCCGAGGATG ATCCGCAGCA GCTGCCGCGA 
CAGATTCACA TTCAGCAGCA CCTCAAACTG CATTTCCACA ACCAAGGCAC AAATGATGGC 
AACAAATATG ATTTGAATGG TTTCTTTGGA AACAGTGGAT ATCAAAGTGA GAACGGACAT 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105416331 ( XM_029837763.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029693623.1 Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo41604
Clone ID Related Accession (Same CDS sequence)	XM_029837763.1
Accession Version	XM_029837763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	filaggrin-2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GGTACTGCAC AGCTAACACA TGGAAACCTG GTTAAGTTGA CACAAGACAA TCATCAGAAG 
CTGACTACCT TTGTACAACA AGCAAAAATC TACACGAACA CACCCATACA AATTACACCA 
CAACCTGTAG CAGTGAATAG ACAGGGCAAA CACCTGGTGC ACACAACTGG ACCCGCACCA 
GAACCAACAC CTGAGGGTAT ACAGGCCATG ACATTCGGAC CTGCACCAGT GCTTCCCCAG 
TCAAACGCGG GACCTGAGCC AACAGAAGCT CTTCCTCATG GTTATGTGGC AGGAAGTGCA 
GCACCTGAGC AAACAGGCAC AGAGGCTGAC ATTGTTTTTG GGGTAGACAG CCACAGTGAA 
CGCACCACAC CACCGCCAAC CAAAGCTCTT CCTGAAGTGG ATGCATTGAA AAGTACAGAA 
GAACCTCTGG CTATGGGTGT GCCTACGGTT ATACCTAAGG AAGAAAATGT CAGGAGTCCA 
ATTGAGTCAG GACCCAAACC ACTTGTGGTC AAAGGGCTTC CTGCAGGAGG AGATGGGGCA 
ACTGTGAAGG GAGAAGCTTC ATCCTTAAAG GGACAGGGAG CTAACCAACA GAGACCTGAC 
TGTGGAGCAC CGCTTGGACA ATGGATGAAA ATACCTAGGC CTGGTTATGT TTCAGGTGTT 
GGAGCATCAT CCAGCATAAA CACAAAAGGG CTTGGACATC CCTATGCTCA GCAACCAGGT 
TATGGACAAG GAGGCTACCT TGGAGCAGGA TATGGAAAAG GAGGAAATGC AGGTTTTGGT 
TCCAAATCAG GATTCAGAAA TGGCTATAAT GCTGGTGTGC AGCCCGACTA CGCCAGTCTT 
GTACCTGCAG CTAACAGCAA ATCAGGAATG AGCGGGGTGC CATTTGGTGG TCTGTCTGCA 
GGAATGGGCG CAGAGAAAAT GAACACAAAA TATGGCATTG GCGGGTTACA GTTTGATGGA 
CAGCAGTTCA ATACTGGGAT TAATGGAGCA GGAAGTTATG GTTATGGTCA AAGCCCACAT 
GGACCCACTG GCCAAGGCAA AACATCAGGA AAATATGGTG GTGTTGGTGC CGGTGCCAGC 
GTGGGGGGCA GTCCTGGAAA ATACGGATTC GGTGGATTTC ACAACAGTCA ACAGGTTTTC 
GGTCTTGGAC CTACTGCAAA TGGAAACTAT GGTTATGGAA GAATGTCCTA TGAACCTGAA 
CCAGCTGTAA TGGGCCCTGA AGCTCCTGGA AATTATGGTC TGACTGCATC ACAATATCAG 
CCCGAACCCC TTGGACTTGG ACACAATGGG CAATTAAGGA GTAATTATGA TGCTGTGCCT 
TATGAAAGCC TGCCTGTTGA GACGGACCCG CTACAAAATC CTTATGTGAA CGGAGACGTA 
CTGACCCCTG TGGGTGTGAG TGTTGAAGGG GATCACTATG AGAACATGGG CTATATCAAT 
GGACAAGTGC AGCCAGAAGA AGCTCCCACT GCCATCCCCT CCCTGGCCTA CCCTGCTCTT 
CCAGCACATC TGCCTGCAGA GTCCTCCCTC ACTCCTGATG TGGGGCTGCC AGCAGAGGTT 
GAGGACTTGC CCGACCCTGC AGGTACTGCC AGCATCCCCC TGGACTCTGA GCCTGCCACA 
GAAACACCAG GCCTGCCTCA GTCGCACGAG CAACCCGAGG ATGATCCGCA GCAGCTGCCG 
CGACAGATTC ACATTCAGCA GCACCTCAAA CTGCATTTCC ACAACCAAGG CACAAATGAT 
GGCAACAAAT ATGATTTGAA TGGTTTCTTT GGAAACAGTG GATATCAAAG TGAGAACGGA 
CATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029693623.1
CDS	344..3772
Translation	MLGRLFLQSVVMVWLAHTAHTWGYGATTGVSNGPANGDTFQKGGGLGSMLMPSKGVGQGVGAQNGYGGYPTKGVGFGAAGAGAATKGWMKGYGAGAARGQPQGGDAAVLSKGNGAKSNGNGAAGVHNGPETKGKGYGSNGFKGYGGEPIRGYASAHGSAGLGLGHQFGNERTKGPTQEYGGSALRSQQGYGSRALPNGYGQNVNRYGQNVKGYGQKTKGYGQNPNGNAQNFDGYGPKPNGYGQTPSRYGQKPNGYGQTPNGNGQKPKGFGQIPNQNGQNVNGYGQKPNGYGQKPNGYGQKLKGYGQTPNRNGQKPNGYGQTPNGYGQKPKGYGQTPNRNGQKPNGYGQTPNGHRQKPNGYGQTSNGYGQKLKGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGHRQKPNGYGQTSNGNGQNLNGHEAAVFKDYGAKSKGHGVSNGAGTGVSGGTNSHNTKGLGTASKSEGNGQTPNAKNMKGMDEVNGRTLKGGVLTPEQPTAVPNMSAIPQQGTTGRPAPGTAQLTHGNLVKLTQDNHQKLTTFVQQAKIYTNTPIQITPQPVAVNRQGKHLVHTTGPAPEPTPEGIQAMTFGPAPVLPQSNAGPEPTEALPHGYVAGSAAPEQTGTEADIVFGVDSHSERTTPPPTKALPEVDALKSTEEPLAMGVPTVIPKEENVRSPIESGPKPLVVKGLPAGGDGATVKGEASSLKGQGANQQRPDCGAPLGQWMKIPRPGYVSGVGASSSINTKGLGHPYAQQPGYGQGGYLGAGYGKGGNAGFGSKSGFRNGYNAGVQPDYASLVPAANSKSGMSGVPFGGLSAGMGAEKMNTKYGIGGLQFDGQQFNTGINGAGSYGYGQSPHGPTGQGKTSGKYGGVGAGASVGGSPGKYGFGGFHNSQQVFGLGPTANGNYGYGRMSYEPEPAVMGPEAPGNYGLTASQYQPEPLGLGHNGQLRSNYDAVPYESLPVETDPLQNPYVNGDVLTPVGVSVEGDHYENMGYINGQVQPEEAPTAIPSLAYPALPAHLPAESSLTPDVGLPAEVEDLPDPAGTASIPLDSEPATETPGLPQSHEQPEDDPQQLPRQIHIQQHLKLHFHNQGTNDGNKYDLNGFFGNSGYQSENGHQ

Target ORF information:

RefSeq Version	XM_029837763.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029837763.1

ORF Insert Sequence:

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GGTACTGCAC AGCTAACACA TGGAAACCTG GTTAAGTTGA CACAAGACAA TCATCAGAAG 
CTGACTACCT TTGTACAACA AGCAAAAATC TACACGAACA CACCCATACA AATTACACCA 
CAACCTGTAG CAGTGAATAG ACAGGGCAAA CACCTGGTGC ACACAACTGG ACCCGCACCA 
GAACCAACAC CTGAGGGTAT ACAGGCCATG ACATTCGGAC CTGCACCAGT GCTTCCCCAG 
TCAAACGCGG GACCTGAGCC AACAGAAGCT CTTCCTCATG GTTATGTGGC AGGAAGTGCA 
GCACCTGAGC AAACAGGCAC AGAGGCTGAC ATTGTTTTTG GGGTAGACAG CCACAGTGAA 
CGCACCACAC CACCGCCAAC CAAAGCTCTT CCTGAAGTGG ATGCATTGAA AAGTACAGAA 
GAACCTCTGG CTATGGGTGT GCCTACGGTT ATACCTAAGG AAGAAAATGT CAGGAGTCCA 
ATTGAGTCAG GACCCAAACC ACTTGTGGTC AAAGGGCTTC CTGCAGGAGG AGATGGGGCA 
ACTGTGAAGG GAGAAGCTTC ATCCTTAAAG GGACAGGGAG CTAACCAACA GAGACCTGAC 
TGTGGAGCAC CGCTTGGACA ATGGATGAAA ATACCTAGGC CTGGTTATGT TTCAGGTGTT 
GGAGCATCAT CCAGCATAAA CACAAAAGGG CTTGGACATC CCTATGCTCA GCAACCAGGT 
TATGGACAAG GAGGCTACCT TGGAGCAGGA TATGGAAAAG GAGGAAATGC AGGTTTTGGT 
TCCAAATCAG GATTCAGAAA TGGCTATAAT GCTGGTGTGC AGCCCGACTA CGCCAGTCTT 
GTACCTGCAG CTAACAGCAA ATCAGGAATG AGCGGGGTGC CATTTGGTGG TCTGTCTGCA 
GGAATGGGCG CAGAGAAAAT GAACACAAAA TATGGCATTG GCGGGTTACA GTTTGATGGA 
CAGCAGTTCA ATACTGGGAT TAATGGAGCA GGAAGTTATG GTTATGGTCA AAGCCCACAT 
GGACCCACTG GCCAAGGCAA AACATCAGGA AAATATGGTG GTGTTGGTGC CGGTGCCAGC 
GTGGGGGGCA GTCCTGGAAA ATACGGATTC GGTGGATTTC ACAACAGTCA ACAGGTTTTC 
GGTCTTGGAC CTACTGCAAA TGGAAACTAT GGTTATGGAA GAATGTCCTA TGAACCTGAA 
CCAGCTGTAA TGGGCCCTGA AGCTCCTGGA AATTATGGTC TGACTGCATC ACAATATCAG 
CCCGAACCCC TTGGACTTGG ACACAATGGG CAATTAAGGA GTAATTATGA TGCTGTGCCT 
TATGAAAGCC TGCCTGTTGA GACGGACCCG CTACAAAATC CTTATGTGAA CGGAGACGTA 
CTGACCCCTG TGGGTGTGAG TGTTGAAGGG GATCACTATG AGAACATGGG CTATATCAAT 
GGACAAGTGC AGCCAGAAGA AGCTCCCACT GCCATCCCCT CCCTGGCCTA CCCTGCTCTT 
CCAGCACATC TGCCTGCAGA GTCCTCCCTC ACTCCTGATG TGGGGCTGCC AGCAGAGGTT 
GAGGACTTGC CCGACCCTGC AGGTACTGCC AGCATCCCCC TGGACTCTGA GCCTGCCACA 
GAAACACCAG GCCTGCCTCA GTCGCACGAG CAACCCGAGG ATGATCCGCA GCAGCTGCCG 
CGACAGATTC ACATTCAGCA GCACCTCAAA CTGCATTTCC ACAACCAAGG CACAAATGAT 
GGCAACAAAT ATGATTTGAA TGGTTTCTTT GGAAACAGTG GATATCAAAG TGAGAACGGA 
CATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105416331 ( XM_029837769.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029693629.1 Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X2, mRNA.

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CloneID	OTo41605
Clone ID Related Accession (Same CDS sequence)	XM_029837769.1
Accession Version	XM_029837769.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	filaggrin-2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GGTACTGCAC AGCTAACACA TGGAAACCTG GTTAAGTTGA CACAAGACAA TCATCAGAAG 
CTGACTACCT TTGTACAACA AGCAAAAATC TACACGAACA CACCCATACA AATTACACCA 
CAACCTGTAG CAGTGAATAG ACAGGGCAAA CACCTGGTGC ACACAACTGG ACCCGCACCA 
GAACCAACAC CTGAGGGTAT ACAGGCCATG ACATTCGGAC CTGCACCAGT GCTTCCCCAG 
TCAAACGCGG GACCTGAGCC AACAGAAGCT CTTCCTCATG GTTATGTGGC AGGAAGTGCA 
GCACCTGAGC AAACAGGCAC AGAGGCTGAC ATTGTTTTTG GGGTAGACAG CCACAGTGAA 
CGCACCACAC CACCGCCAAC CAAAGCTCTT CCTGAAGTGG ATGCATTGAA AAGTACAGAA 
GAACCTCTGG CTATGGGTGT GCCTACGGTT ATACCTAAGG AAGAAAATGT CAGGAGTCCA 
ATTGAGTCAG GACCCAAACC ACTTGTGGTC AAAGGGCTTC CTGCAGGAGG AGATGGGGCA 
ACTGTGAAGG GAGAAGCTTC ATCCTTAAAG GGACAGGGAG CTAACCAACA GAGACCTGAC 
TGTGGAGCAC CGCTTGGACA ATGGATGAAA ATACCTAGGC CTGGTTATGT TTCAGGTGTT 
GGAGCATCAT CCAGCATAAA CACAAAAGGG CTTGGACATC CCTATGCTCA GCAACCAGGT 
TATGGACAAG GAGGCTACCT TGGAGCAGGA TATGGAAAAG GAGGAAATGC AGGTTTTGGT 
TCCAAATCAG GATTCAGAAA TGGCTATAAT GCTGGTGTGC AGCCCGACTA CGCCAGTCTT 
GTACCTGCAG CTAACAGCAA ATCAGGAATG AGCGGGGTGC CATTTGGTGG TCTGTCTGCA 
GGAATGGGCG CAGAGAAAAT GAACACAAAA TATGGCATTG GCGGGTTACA GTTTGATGGA 
CAGCAGTTCA ATACTGGGAT TAATGGAGCA GGAAGTTATG GTTATGGTCA AAGCCCACAT 
GGACCCACTG GCCAAGGCAA AACATCAGGT GGTGTTGGTG CCGGTGCCAG CGTGGGGGGC 
AGTCCTGGAA AATACGGATT CGGTGGATTT CACAACAGTC AACAGGTTTT CGGTCTTGGA 
CCTACTGCAA ATGGAAACTA TGGTTATGGA AGAATGTCCT ATGAACCTGA ACCAGCTGTA 
ATGGGCCCTG AAGCTCCTGG AAATTATGGT CTGACTGCAT CACAATATCA GCCCGAACCC 
CTTGGACTTG GACACAATGG GCAATTAAGG AGTAATTATG ATGCTGTGCC TTATGAAAGC 
CTGCCTGTTG AGACGGACCC GCTACAAAAT CCTTATGTGA ACGGAGACGT ACTGACCCCT 
GTGGGTGTGA GTGTTGAAGG GGATCACTAT GAGAACATGG GCTATATCAA TGGACAAGTG 
CAGCCAGAAG AAGCTCCCAC TGCCATCCCC TCCCTGGCCT ACCCTGCTCT TCCAGCACAT 
CTGCCTGCAG AGTCCTCCCT CACTCCTGAT GTGGGGCTGC CAGCAGAGGT TGAGGACTTG 
CCCGACCCTG CAGGTACTGC CAGCATCCCC CTGGACTCTG AGCCTGCCAC AGAAACACCA 
GGCCTGCCTC AGTCGCACGA GCAACCCGAG GATGATCCGC AGCAGCTGCC GCGACAGATT 
CACATTCAGC AGCACCTCAA ACTGCATTTC CACAACCAAG GCACAAATGA TGGCAACAAA 
TATGATTTGA ATGGTTTCTT TGGAAACAGT GGATATCAAA GTGAGAACGG ACATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029693629.1
CDS	345..3764
Translation	MLGRLFLQSVVMVWLAHTAHTWGYGATTGVSNGPANGDTFQKGGGLGSMLMPSKGVGQGVGAQNGYGGYPTKGVGFGAAGAGAATKGWMKGYGAGAARGQPQGGDAAVLSKGNGAKSNGNGAAGVHNGPETKGKGYGSNGFKGYGGEPIRGYASAHGSAGLGLGHQFGNERTKGPTQEYGGSALRSQQGYGSRALPNGYGQNVNRYGQNVKGYGQKTKGYGQNPNGNAQNFDGYGPKPNGYGQTPSRYGQKPNGYGQTPNGNGQKPKGFGQIPNQNGQNVNGYGQKPNGYGQKPNGYGQKLKGYGQTPNRNGQKPNGYGQTPNGYGQKPKGYGQTPNRNGQKPNGYGQTPNGHRQKPNGYGQTSNGYGQKLKGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGHRQKPNGYGQTSNGNGQNLNGHEAAVFKDYGAKSKGHGVSNGAGTGVSGGTNSHNTKGLGTASKSEGNGQTPNAKNMKGMDEVNGRTLKGGVLTPEQPTAVPNMSAIPQQGTTGRPAPGTAQLTHGNLVKLTQDNHQKLTTFVQQAKIYTNTPIQITPQPVAVNRQGKHLVHTTGPAPEPTPEGIQAMTFGPAPVLPQSNAGPEPTEALPHGYVAGSAAPEQTGTEADIVFGVDSHSERTTPPPTKALPEVDALKSTEEPLAMGVPTVIPKEENVRSPIESGPKPLVVKGLPAGGDGATVKGEASSLKGQGANQQRPDCGAPLGQWMKIPRPGYVSGVGASSSINTKGLGHPYAQQPGYGQGGYLGAGYGKGGNAGFGSKSGFRNGYNAGVQPDYASLVPAANSKSGMSGVPFGGLSAGMGAEKMNTKYGIGGLQFDGQQFNTGINGAGSYGYGQSPHGPTGQGKTSGGVGAGASVGGSPGKYGFGGFHNSQQVFGLGPTANGNYGYGRMSYEPEPAVMGPEAPGNYGLTASQYQPEPLGLGHNGQLRSNYDAVPYESLPVETDPLQNPYVNGDVLTPVGVSVEGDHYENMGYINGQVQPEEAPTAIPSLAYPALPAHLPAESSLTPDVGLPAEVEDLPDPAGTASIPLDSEPATETPGLPQSHEQPEDDPQQLPRQIHIQQHLKLHFHNQGTNDGNKYDLNGFFGNSGYQSENGHQ

Target ORF information:

RefSeq Version	XM_029837769.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029837769.1

ORF Insert Sequence:

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GGTACTGCAC AGCTAACACA TGGAAACCTG GTTAAGTTGA CACAAGACAA TCATCAGAAG 
CTGACTACCT TTGTACAACA AGCAAAAATC TACACGAACA CACCCATACA AATTACACCA 
CAACCTGTAG CAGTGAATAG ACAGGGCAAA CACCTGGTGC ACACAACTGG ACCCGCACCA 
GAACCAACAC CTGAGGGTAT ACAGGCCATG ACATTCGGAC CTGCACCAGT GCTTCCCCAG 
TCAAACGCGG GACCTGAGCC AACAGAAGCT CTTCCTCATG GTTATGTGGC AGGAAGTGCA 
GCACCTGAGC AAACAGGCAC AGAGGCTGAC ATTGTTTTTG GGGTAGACAG CCACAGTGAA 
CGCACCACAC CACCGCCAAC CAAAGCTCTT CCTGAAGTGG ATGCATTGAA AAGTACAGAA 
GAACCTCTGG CTATGGGTGT GCCTACGGTT ATACCTAAGG AAGAAAATGT CAGGAGTCCA 
ATTGAGTCAG GACCCAAACC ACTTGTGGTC AAAGGGCTTC CTGCAGGAGG AGATGGGGCA 
ACTGTGAAGG GAGAAGCTTC ATCCTTAAAG GGACAGGGAG CTAACCAACA GAGACCTGAC 
TGTGGAGCAC CGCTTGGACA ATGGATGAAA ATACCTAGGC CTGGTTATGT TTCAGGTGTT 
GGAGCATCAT CCAGCATAAA CACAAAAGGG CTTGGACATC CCTATGCTCA GCAACCAGGT 
TATGGACAAG GAGGCTACCT TGGAGCAGGA TATGGAAAAG GAGGAAATGC AGGTTTTGGT 
TCCAAATCAG GATTCAGAAA TGGCTATAAT GCTGGTGTGC AGCCCGACTA CGCCAGTCTT 
GTACCTGCAG CTAACAGCAA ATCAGGAATG AGCGGGGTGC CATTTGGTGG TCTGTCTGCA 
GGAATGGGCG CAGAGAAAAT GAACACAAAA TATGGCATTG GCGGGTTACA GTTTGATGGA 
CAGCAGTTCA ATACTGGGAT TAATGGAGCA GGAAGTTATG GTTATGGTCA AAGCCCACAT 
GGACCCACTG GCCAAGGCAA AACATCAGGT GGTGTTGGTG CCGGTGCCAG CGTGGGGGGC 
AGTCCTGGAA AATACGGATT CGGTGGATTT CACAACAGTC AACAGGTTTT CGGTCTTGGA 
CCTACTGCAA ATGGAAACTA TGGTTATGGA AGAATGTCCT ATGAACCTGA ACCAGCTGTA 
ATGGGCCCTG AAGCTCCTGG AAATTATGGT CTGACTGCAT CACAATATCA GCCCGAACCC 
CTTGGACTTG GACACAATGG GCAATTAAGG AGTAATTATG ATGCTGTGCC TTATGAAAGC 
CTGCCTGTTG AGACGGACCC GCTACAAAAT CCTTATGTGA ACGGAGACGT ACTGACCCCT 
GTGGGTGTGA GTGTTGAAGG GGATCACTAT GAGAACATGG GCTATATCAA TGGACAAGTG 
CAGCCAGAAG AAGCTCCCAC TGCCATCCCC TCCCTGGCCT ACCCTGCTCT TCCAGCACAT 
CTGCCTGCAG AGTCCTCCCT CACTCCTGAT GTGGGGCTGC CAGCAGAGGT TGAGGACTTG 
CCCGACCCTG CAGGTACTGC CAGCATCCCC CTGGACTCTG AGCCTGCCAC AGAAACACCA 
GGCCTGCCTC AGTCGCACGA GCAACCCGAG GATGATCCGC AGCAGCTGCC GCGACAGATT 
CACATTCAGC AGCACCTCAA ACTGCATTTC CACAACCAAG GCACAAATGA TGGCAACAAA 
TATGATTTGA ATGGTTTCTT TGGAAACAGT GGATATCAAA GTGAGAACGG ACATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105416331 ( XM_029837774.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029693634.1 Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X3, mRNA.

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CloneID	OTo41606
Clone ID Related Accession (Same CDS sequence)	XM_029837774.1
Accession Version	XM_029837774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	fibroin heavy chain-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GGTACTGCAC AGCTAACACA TGGAAACCTG GTTAAGTTGA CACAAGACAA TCATCAGAAG 
CTGACTACCT TTGTACAACA AGCAAAAATC TACACGAACA CACCCATACA AATTACACCA 
CAACCTGTAG CAGTGAATAG ACAGGGCAAA CACCTGGTGC ACACAACTGG ACCCGCACCA 
GAACCAACAC CTGAGGGTAT ACAGGCCATG ACATTCGGAC CTGCACCAGT GCTTCCCCAG 
TCAAACGCGG GACCTGAGCC AACAGAAGCT CTTCCTCATG TGAAGGGAGA AGCTTCATCC 
TTAAAGGGAC AGGGAGCTAA CCAACAGAGA CCTGACTGTG GAGCACCGCT TGGACAATGG 
ATGAAAATAC CTAGGCCTGG TTATGTTTCA GGTGTTGGAG CATCATCCAG CATAAACACA 
AAAGGGCTTG GACATCCCTA TGCTCAGCAA CCAGGTTATG GACAAGGAGG CTACCTTGGA 
GCAGGATATG GAAAAGGAGG AAATGCAGGT TTTGGTTCCA AATCAGGATT CAGAAATGGC 
TATAATGCTG GTGTGCAGCC CGACTACGCC AGTCTTGTAC CTGCAGCTAA CAGCAAATCA 
GGAATGAGCG GGGTGCCATT TGGTGGTCTG TCTGCAGGAA TGGGCGCAGA GAAAATGAAC 
ACAAAATATG GCATTGGCGG GTTACAGTTT GATGGACAGC AGTTCAATAC TGGGATTAAT 
GGAGCAGGAA GTTATGGTTA TGGTCAAAGC CCACATGGAC CCACTGGCCA AGGCAAAACA 
TCAGGAAAAT ATGGTGGTGT TGGTGCCGGT GCCAGCGTGG GGGGCAGTCC TGGAAAATAC 
GGATTCGGTG GATTTCACAA CAGTCAACAG GTTTTCGGTC TTGGACCTAC TGCAAATGGA 
AACTATGGTT ATGGAAGAAT GTCCTATGAA CCTGAACCAG CTGTAATGGG CCCTGAAGCT 
CCTGGAAATT ATGGTCTGAC TGCATCACAA TATCAGCCCG AACCCCTTGG ACTTGGACAC 
AATGGGCAAT TAAGGAGTAA TTATGATGCT GTGCCTTATG AAAGCCTGCC TGTTGAGACG 
GACCCGCTAC AAAATCCTTA TGTGAACGGA GACGTACTGA CCCCTGTGGG TGTGAGTGTT 
GAAGGGGATC ACTATGAGAA CATGGGCTAT ATCAATGGAC AAGTGCAGCC AGAAGAAGCT 
CCCACTGCCA TCCCCTCCCT GGCCTACCCT GCTCTTCCAG CACATCTGCC TGCAGAGTCC 
TCCCTCACTC CTGATGTGGG GCTGCCAGCA GAGGTTGAGG ACTTGCCCGA CCCTGCAGGT 
ACTGCCAGCA TCCCCCTGGA CTCTGAGCCT GCCACAGAAA CACCAGGCCT GCCTCAGTCG 
CACGAGCAAC CCGAGGATGA TCCGCAGCAG CTGCCGCGAC AGATTCACAT TCAGCAGCAC 
CTCAAACTGC ATTTCCACAA CCAAGGCACA AATGATGGCA ACAAATATGA TTTGAATGGT 
TTCTTTGGAA ACAGTGGATA TCAAAGTGAG AACGGACATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029693634.1
CDS	341..3505
Translation	MLGRLFLQSVVMVWLAHTAHTWGYGATTGVSNGPANGDTFQKGGGLGSMLMPSKGVGQGVGAQNGYGGYPTKGVGFGAAGAGAATKGWMKGYGAGAARGQPQGGDAAVLSKGNGAKSNGNGAAGVHNGPETKGKGYGSNGFKGYGGEPIRGYASAHGSAGLGLGHQFGNERTKGPTQEYGGSALRSQQGYGSRALPNGYGQNVNRYGQNVKGYGQKTKGYGQNPNGNAQNFDGYGPKPNGYGQTPSRYGQKPNGYGQTPNGNGQKPKGFGQIPNQNGQNVNGYGQKPNGYGQKPNGYGQKLKGYGQTPNRNGQKPNGYGQTPNGYGQKPKGYGQTPNRNGQKPNGYGQTPNGHRQKPNGYGQTSNGYGQKLKGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGNGQKPNGYGQTPNGHRQKPNGYGQTSNGNGQNLNGHEAAVFKDYGAKSKGHGVSNGAGTGVSGGTNSHNTKGLGTASKSEGNGQTPNAKNMKGMDEVNGRTLKGGVLTPEQPTAVPNMSAIPQQGTTGRPAPGTAQLTHGNLVKLTQDNHQKLTTFVQQAKIYTNTPIQITPQPVAVNRQGKHLVHTTGPAPEPTPEGIQAMTFGPAPVLPQSNAGPEPTEALPHVKGEASSLKGQGANQQRPDCGAPLGQWMKIPRPGYVSGVGASSSINTKGLGHPYAQQPGYGQGGYLGAGYGKGGNAGFGSKSGFRNGYNAGVQPDYASLVPAANSKSGMSGVPFGGLSAGMGAEKMNTKYGIGGLQFDGQQFNTGINGAGSYGYGQSPHGPTGQGKTSGKYGGVGAGASVGGSPGKYGFGGFHNSQQVFGLGPTANGNYGYGRMSYEPEPAVMGPEAPGNYGLTASQYQPEPLGLGHNGQLRSNYDAVPYESLPVETDPLQNPYVNGDVLTPVGVSVEGDHYENMGYINGQVQPEEAPTAIPSLAYPALPAHLPAESSLTPDVGLPAEVEDLPDPAGTASIPLDSEPATETPGLPQSHEQPEDDPQQLPRQIHIQQHLKLHFHNQGTNDGNKYDLNGFFGNSGYQSENGHQ

Target ORF information:

RefSeq Version	XM_029837774.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes spidroin-1-like (LOC105416331), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029837774.1

ORF Insert Sequence:

ATGCTGGGAC GGCTGTTTCT GCAGAGTGTG GTCATGGTTT GGCTGGCACA CACAGCACAC 
ACATGGGGTT ATGGTGCCAC AACTGGGGTC TCAAATGGGC CAGCTAATGG AGATACATTT 
CAAAAAGGTG GAGGTCTTGG AAGCATGTTG ATGCCATCAA AAGGTGTGGG TCAGGGAGTG 
GGTGCACAAA ATGGATATGG TGGATACCCC ACCAAAGGAG TAGGCTTTGG TGCAGCTGGA 
GCTGGAGCAG CTACCAAAGG ATGGATGAAA GGCTATGGGG CTGGTGCAGC TAGGGGACAA 
CCACAGGGAG GAGATGCTGC AGTTTTGTCC AAAGGAAATG GAGCCAAATC TAATGGTAAT 
GGTGCAGCTG GTGTACATAA TGGACCTGAA ACAAAAGGCA AAGGGTATGG CAGTAATGGT 
TTTAAGGGCT ATGGAGGTGA ACCCATTAGA GGATATGCTT CTGCTCATGG CAGTGCTGGT 
TTGGGTCTGG GACATCAGTT TGGAAATGAA AGAACAAAGG GACCGACGCA AGAATATGGT 
GGCAGTGCCC TAAGATCCCA ACAAGGTTAT GGAAGTAGAG CTCTTCCTAA TGGATATGGG 
CAAAATGTAA ATAGATATGG CCAAAATGTG AAAGGATATG GGCAAAAAAC TAAAGGATAT 
GGCCAAAATC CCAATGGAAA TGCCCAAAAT TTCGATGGAT ATGGTCCAAA ACCCAATGGA 
TATGGTCAAA CTCCGAGTAG ATATGGACAG AAACCTAATG GATATGGCCA AACTCCTAAT 
GGCAATGGTC AGAAACCCAA GGGGTTTGGC CAAATTCCTA ATCAAAATGG ACAAAATGTG 
AATGGATATG GCCAAAAACC CAATGGATAC GGCCAAAAAC CCAATGGATA TGGGCAGAAA 
CTGAAGGGCT ATGGCCAAAC TCCGAACAGA AATGGACAAA AACCCAATGG ATATGGCCAA 
ACTCCAAATG GATATGGGCA GAAACCGAAG GGATATGGCC AAACTCCGAA CAGAAATGGA 
CAAAAACCCA ATGGATATGG CCAAACTCCT AACGGACATA GGCAAAAACC CAATGGATAT 
GGCCAAACTT CAAATGGATA TGGGCAGAAA CTAAAGGGAT ATGGCCAAAC TCCAAATGGA 
AATGGGCAAA AACCCAATGG ATATGGCCAA ACTCCAAATG GAAATGGACA AAAACCCAAT 
GGATATGGCC AAACTCCAAA TGGAAATGGA CAAAAACCCA ATGGATATGG CCAAACTCCT 
AACGGACATA GGCAAAAACC CAATGGATAT GGCCAAACTT CAAATGGAAA TGGACAAAAT 
CTTAATGGGC ATGAAGCTGC TGTTTTTAAA GACTATGGAG CCAAATCCAA AGGTCACGGA 
GTCAGTAATG GTGCTGGAAC TGGTGTATCT GGAGGCACAA ATTCTCATAA TACTAAAGGA 
CTTGGAACCG CTTCAAAAAG TGAAGGCAAT GGCCAGACTC CAAATGCAAA GAATATGAAG 
GGCATGGATG AAGTGAATGG ACGGACTCTT AAAGGTGGAG TTCTTACTCC AGAACAGCCA 
ACAGCAGTAC CAAATATGAG TGCCATCCCT CAACAGGGAA CAACTGGCAG ACCAGCACCA 
GGTACTGCAC AGCTAACACA TGGAAACCTG GTTAAGTTGA CACAAGACAA TCATCAGAAG 
CTGACTACCT TTGTACAACA AGCAAAAATC TACACGAACA CACCCATACA AATTACACCA 
CAACCTGTAG CAGTGAATAG ACAGGGCAAA CACCTGGTGC ACACAACTGG ACCCGCACCA 
GAACCAACAC CTGAGGGTAT ACAGGCCATG ACATTCGGAC CTGCACCAGT GCTTCCCCAG 
TCAAACGCGG GACCTGAGCC AACAGAAGCT CTTCCTCATG TGAAGGGAGA AGCTTCATCC 
TTAAAGGGAC AGGGAGCTAA CCAACAGAGA CCTGACTGTG GAGCACCGCT TGGACAATGG 
ATGAAAATAC CTAGGCCTGG TTATGTTTCA GGTGTTGGAG CATCATCCAG CATAAACACA 
AAAGGGCTTG GACATCCCTA TGCTCAGCAA CCAGGTTATG GACAAGGAGG CTACCTTGGA 
GCAGGATATG GAAAAGGAGG AAATGCAGGT TTTGGTTCCA AATCAGGATT CAGAAATGGC 
TATAATGCTG GTGTGCAGCC CGACTACGCC AGTCTTGTAC CTGCAGCTAA CAGCAAATCA 
GGAATGAGCG GGGTGCCATT TGGTGGTCTG TCTGCAGGAA TGGGCGCAGA GAAAATGAAC 
ACAAAATATG GCATTGGCGG GTTACAGTTT GATGGACAGC AGTTCAATAC TGGGATTAAT 
GGAGCAGGAA GTTATGGTTA TGGTCAAAGC CCACATGGAC CCACTGGCCA AGGCAAAACA 
TCAGGAAAAT ATGGTGGTGT TGGTGCCGGT GCCAGCGTGG GGGGCAGTCC TGGAAAATAC 
GGATTCGGTG GATTTCACAA CAGTCAACAG GTTTTCGGTC TTGGACCTAC TGCAAATGGA 
AACTATGGTT ATGGAAGAAT GTCCTATGAA CCTGAACCAG CTGTAATGGG CCCTGAAGCT 
CCTGGAAATT ATGGTCTGAC TGCATCACAA TATCAGCCCG AACCCCTTGG ACTTGGACAC 
AATGGGCAAT TAAGGAGTAA TTATGATGCT GTGCCTTATG AAAGCCTGCC TGTTGAGACG 
GACCCGCTAC AAAATCCTTA TGTGAACGGA GACGTACTGA CCCCTGTGGG TGTGAGTGTT 
GAAGGGGATC ACTATGAGAA CATGGGCTAT ATCAATGGAC AAGTGCAGCC AGAAGAAGCT 
CCCACTGCCA TCCCCTCCCT GGCCTACCCT GCTCTTCCAG CACATCTGCC TGCAGAGTCC 
TCCCTCACTC CTGATGTGGG GCTGCCAGCA GAGGTTGAGG ACTTGCCCGA CCCTGCAGGT 
ACTGCCAGCA TCCCCCTGGA CTCTGAGCCT GCCACAGAAA CACCAGGCCT GCCTCAGTCG 
CACGAGCAAC CCGAGGATGA TCCGCAGCAG CTGCCGCGAC AGATTCACAT TCAGCAGCAC 
CTCAAACTGC ATTTCCACAA CCAAGGCACA AATGATGGCA ACAAATATGA TTTGAATGGT 
TTCTTTGGAA ACAGTGGATA TCAAAGTGAG AACGGACATC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC105416331 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム