pikfyve gene cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

mRNA	Protein	Name
XM_011607509.1	XP_011605811.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_011607520.1	XP_011605822.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_003961636.2	XP_003961685.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_003961635.2	XP_003961684.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_011607533.1	XP_011605835.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
XM_011607487.2	XP_011605789.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
XM_011607495.2	XP_011605797.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
XM_011607533.2	XP_011605835.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X17
XM_011607487.1	XP_011605789.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_011607495.1	XP_011605797.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_029832594.1	XP_029688454.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
XM_029832611.1	XP_029688471.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
XM_029832613.1	XP_029688473.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
XM_029832622.1	XP_029688482.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
XM_029832598.1	XP_029688458.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_029832606.1	XP_029688466.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
XM_029832636.1	XP_029688496.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X12
XM_029832648.1	XP_029688508.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
XM_011607513.1	XP_011605815.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
XM_029832587.1	XP_029688447.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
XM_029832590.1	XP_029688450.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
XM_029832601.1	XP_029688461.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
XM_029832602.1	XP_029688462.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
XM_029832639.1	XP_029688499.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X13
XM_029832642.1	XP_029688502.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X14
XM_029832644.1	XP_029688504.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X15
XM_011607503.1	XP_011605805.1	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo40936	XM_011607533.2 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo31331	XM_011607533.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OTo40920	XM_029832587.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40921	XM_029832590.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40922	XM_029832594.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40923	XM_029832598.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40924	XM_029832601.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40925	XM_029832602.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40926	XM_029832606.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40927	XM_029832611.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40928	XM_029832613.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40929	XM_029832622.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40930	XM_011607495.2 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40930	XM_011607487.2 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40931	XM_029832636.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40932	XM_029832639.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40933	XM_029832642.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40934	XM_029832644.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo40935	XM_029832648.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo00680	XM_003961635.2 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OTo00681	XM_003961636.2 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OTo31326	XM_011607487.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OTo31326	XM_011607495.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OTo31327	XM_011607503.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OTo31328	XM_011607509.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OTo31329	XM_011607513.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OTo31330	XM_011607520.1 Latest version!	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

pikfyve ( XM_011607533.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605835.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OTo40936
Clone ID Related Accession (Same CDS sequence)	XM_011607533.2
Accession Version	XM_011607533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011607533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGCCAGGA CAGTGACTTG AAGCAGTACT GGATGCCGGA CAGCCAGTGT 
AAAGAATGTT ATGACTGCAA TGAAAAGTTC ACAACCTTCC GACGCCGCCA CCATTGCCGA 
CTGTGTGGTC AGATTTTCTG CAGTCGCTGC TGCAACCAGG AAATCCCTGG AAAGTTCATG 
GGTTACACAG GAGATTTGCG GGCCTGTACG TACTGCCGTA AGTTGGCACT GAGCTACGCC 
CACTCGGCTG ATTCGGGCTC CATCGGCGAA GACTTGAACA CCCTGACTGA CTCTCCGAGC 
TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA CGGACGCCTG TGGGGGGGCG CAAGGCCAGC 
AGGAACATCT TCCTAGAGGA AGACTTAACA TGGCAGAGAA AAACACCTAT TGGGATGAGA 
AAGAACATGA TTCACCAGGA ACCTCAGAGC AGCGGCCTCA GTTCCAGACT GACAGGACTT 
CAGGAGGATG TAGGAAAATC ACCTGGTAGG AGGAGGTCTG CTAGCATCAG CACACTTCCA 
CTTGACCGCT CTGGGTCATC AATGGTACCT TCCTACGACA GCTCCGTCAG CCCCCCTACC 
AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG ACAAAATTAG ATCACAGTGA GGAGGAACGT 
AAAATACTAC TGGACTCCTC CCAGTTGAAG GACTTATGGA GGAAAATCTG TCATAATGGA 
ACAGGGATGG AATTCCAGGA CCACAGATAC TGGCTGAGAA CCTACCCCAA CTGCATTGTG 
GGAAAAGAGC TCGTCAACTG GCTGCTAAGA AATGGCACCA TTTCCAACAG GGTCCAAGCT 
ATTGCCATAG GTCAGGCACT CGTGGATGGC CGCTGGCTCG ACTGTGTCAC CCACCACGAT 
CAGCTATTCA GAGATGAGTA TGCTCTCTAT CGACCACTTC AGAGTACAGA GTTCTCAGAA 
ACTCCCTCCC CGGACAGCGA TAGTGTCAAC TCACTAGAGG GACATTCAGA GCCGTCATGG 
TTCAAGGACA TCAAATTCGA TGACAGCGAC ACCGAGCAGC CCGCAGACGA CACAGAGTAC 
ACCATGCCTA GTTCCGCTGG GCCCAGCAAG AGGACATCTG TCAGCAGCGT CCACTCAGCA 
GTAGACAGTG ACTCAGCTGC CTCCATCAAC CTCAACATGG AGCAAAACAA TGTGAATTTT 
CGAATCAAGA AAGATTCCAA GTGTCCCCAG GCATTCCCCG CCCTGGAGCA CAAATCTGAA 
ATTATGGCGT CTGAAGATGG AGGAGAGAAC ATCCTTATAA GTGATGCCTT TATCAAAGAG 
TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG GCTAAAGAAA TGCTGTTTAC TCCACTGGGT 
TGGCACCACA GCTCTCTGGA CCAGCTCAGA GAGGAGAATG GAGAAAAGAA GGCTATGGAG 
AGGCTGCTCT CGGCAAATCA CAGCCACATG ATGGCGCTGC TGCAGCAGTT GCTCTACAGC 
GAGTCTTTGT CTCTGTCCTG GCGGGATATT ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG 
ACAGTTCGAC CTGATGTTCG CAACAGTGAT GACGACATGG ACATCCGCCA ACTGGTCCAT 
ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT GACTCAACTG TAGTGAATGG CTTTGTGTGT 
ACGAAGAGTA TTGCCCACAA AAAGATGAAC CCATATATCA AGAACCCCAA AATTCTGCTG 
CTGAAATGCT CAATAGAGTA TCTGTACAGG GAAGAGACCA AATTCACGTC CATCGACCCC 
ATCGTGCTGC AGGAACGAGA GTTTCTGAAA AACTATGTGC AACGTATCGT GGATGTTCGG 
CCCACTCTGG TTCTGGTGGA AAAAACGGTG TCTCGTATAG CTCAGGACAT GCTGCTAGAA 
CACGGCATTA CCCTGGTCAT TAATGTCAAA CCGCAAGTAT TGGACAGGGT GAGTTGTGTG 
ACCCAGGGGG ACCTTGTCAT GTCCATGGAC CAGCTACTCA CCAAACCTCG TCTGGGTACC 
TGCCACAAGT TTTACATGCA GCCCTTCCCT TTGGCCAATA ATGAGGTCAA GACTCTGATG 
TTCTTCGAAG GATGTCCTGC TCATCTCGGG TGCTCCATAA ATCTACGTGG TGCTTCAGAC 
TACGAGTTAG CCAGGGTGAA GGAGATCATG CGATTAATGG TGTGTGTGGC CTACCACTCT 
CAGCTGGAGA TCTCCTTCCT CATGGATGAG TTTGCCATGC CGCCCAGCTT GTCCCAGAGC 
ACTTCATTTC CCTGTCTCCT CGACGGAGGC TCTGCAGATG AGGAAGCAAG CAGGGAGAAA 
CTAGAGAAGA GTTCTGAAGA TGAAGACACT GTAGTGGAAG GACAACCTGA GGAGGTGCCC 
TCTGCCAATG ATGTAGACAC TTCCCAGGAC ACAAATGATC CTAAATTATT CTCCTCTATG 
GTGGAAGAGA CCACTAATAC TGACACAGTC ACGACAAAGT CTTCCACTCC GTTCCAAAGC 
CCTCGTGCGC CATGCTTGCC AGTTCCTCCA CCATTCATCA TGGAAGAAAC CCCAGAAATG 
GGCTCAGACA GCCTAACAGG GGCATTTGAG GCAGGGGTGG TGGAATGCTG CCCAAAGGAG 
GAATCAACCA ACCTAGAAGA TGGAGAAGAT TCTGATATTC CTACTCCTAA GTTGTTCCGA 
GACCCCCTGC AAGATGATAC TGGGATGTTT GTGGCTGAAG AAGTTGCTTC TTCAGATGAC 
CATCTCAAGT CCATCTCTGC TATCTTCAAA CAGGAACTTA AGGACATCAT CCTGTGCATT 
TCACCTTTCA TCACATTTGA GAAACCATTT CTTCTTACCC CTGCTGGGAT GCACTGCCCG 
AGCAGGGATT ATTTTCCCGA ACAGGTCTAT CTGTCTCCTC TCCTCAACAA GGACTTTAAA 
GAGTTGGATG GACGCAGAAA GCGGCAGCTC CTTAAGGAGT CCACTCCCTC CCTGGCAAAC 
AGCCTAACGA ATGGTGGCCC CCCGTCAAAG CATGTCAGTG TCCTGCCATC CCACAGTCTC 
ACCAGCACCC GGATCGTTGA ACTAGTAAAC AGGAGCCAGG GTCTGGCCAG AATGGTTGCA 
GACTACAGAG CAAAGGGTGG ACGTATCCAG CACAGCGAAG CTACTGATCC ATTTTGCATA 
AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG AATAGACCAG GTGATGGTTC GGTCAGAGCA 
CCAGTAAAGA CTGACAGCGA AGAGGAGAAG CCAACTAAAC CCAGTGACTG GACCCCCAAG 
CTGGATTGTC TGAACCCCAC CAACCACCAG AGACTCTGTG TGCTTTTTAG CAGTTCATCA 
ACTCAGTCCA GCAATGCCCC CAACCCCTGT GTCAACCCAT GGATTGTTAC CATGGAGTTT 
TATGGCAAGA ATGACCTCTG CCTTGGTGTA TTCTTGGAGC GATATTGCTT TAGGCCATCA 
TACCAGTGCC CCAGTATATT TTGTGAGACC CCCATGGTGC ACCACATCCG GCGTTTTGTC 
CATGGCAACG GCTGCGTGCA GATAGTACTG AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC 
CAGCACACCA TTCTCAACTA CTCCTGGTGC CGCATCTGCA AACAAGTGAC ACCAGTGGTT 
CCTCTATCCA ATGACTCATG GTCAATGTCT TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC 
GGCCACCAGT ACACCAGGAG GGCCAATGCA GAGCCCTGTG GCCACTCCAT CCACAAAGAC 
TACCACCAGT ACTTCTCATA CAACCAAATG GTGGCTTCCT TCAGTTACAC CTCAGTGAGG 
CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA ATCTTCATCA GGAATCAGGG TCCTTCGATC 
AACAACTTGG GGCAGGATCT GAAAGACTTC TCTCAAAAAG TAAATCAGGT TTACGTGGCC 
ATAGATGATC GTCTCACATC TCTAAAGACG GACACCTTCA GTAAAACACG CGAGGAGAAG 
ATGGAGGACC TCTTTGCACA GAAAGATATG GAAGAGGCGG AACTGCGCAG CTGGATTGAA 
AAGTTTCAAG CACGATTGCA GGCTTGTGGT CACGTTTCTC CTCAGCAGCT GCAGACGACT 
CTGGAATCAC TGGTGATGAA GAAACAGAGT CTGTGTGAGA TGTTGCAGTA CTGGAACAGC 
AGGCTGCAGG AATTATTCCA ACAGGAGAAA GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC 
CCCGGCAGGC ACAGACAGAC TGCCCCTGAT GATGGCAAGA ACGCTGTGGA GTCATCCCCA 
CGTAACCCTT CACCTGTGGT ACAGAATGGT GAAAAGGAGG ACCGTCACCT CACCACACTG 
CCTTCAACCT CCTCTCTGTT GCCCTCGCCT GGAGACCTGG GAGCTGAACC AGCCACATCT 
GGTCCTTCCC TGAGTGAGCA GGACTCTGTC AGCATCCCAG AAGATGTATT TGATGGACAC 
TTGCTGGGCT CCAATGACAG TCAGGTGAAG GAGAAATCCA CCATGAAAGC CATCCTTGCC 
AACTTCCTGC CCGGCAACAG TTACAACCCC ATTCCATTCC CATTTGACCC AGACAAACAC 
TACCTGATGG ATGAGTATGA GCGAGTGCCA ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC 
ATCATTGCAT TTGCTCTGAG TTGTAAGAAG TATAAAACAG CACTGGAAGA TCTGTCCAAG 
GTGTCAAACA TGGGTGGAGA AGACTCTTCA CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG 
AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA GCCTCGTCCC AACAGAGCCG CAGCAGCAGT 
GAAGCAGATC CCCTCAGTAG GCGTCCAGAA GACGCAGACC TAACAGACAA ACAGAAGAAG 
CAGGCCCTGA ATCCCCACAT TGAGCTACAG TTCTCTGATG CAAGCGCAAA GTTTTACTGT 
CGGATCTACT ACGCCGAGGA GTTTCATAAG ATGCGTGAGG AGATCATGGA GAGCTCCGAA 
GAGGACTTTG TGCGTTCCCT GTCGCACTGT GTCAACTGGC AGGCGCGCGG TGGAAAGTCT 
GGGGCCGTTT TCTACGCAAC AGAAGACGAC CGTTTCATCC TAAAACAGAT GCCCAGGCTG 
GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG CACTACTTCA CCTACATCAC CGGAGCTGTG 
CAGCAGAAGA GGCCAACTGC ATTGGCAAAG ATTCTCGGAG TTTACAGGAT AGGCTACAAG 
AACTCCCAGA ACAACACTGA GAAGAAACTG GACCTGCTCG TCATGGAAAA CCTGTTCTAC 
GGACGCAAGA TGGCTCAGGT CTTTGACCTG AAAGGTTCCC TCAGAAACCG TCATGTGAAG 
ACAGACCTGG TAAAGGAAAG CTGCGAGGTG GTCCTGCTGG ATGAGAATCT ACTGAAGCTG 
ATCCACGACA ATCCGCTCTA CATTCGCTCT CACTGCAAGT CCATCTTGCG CGCCGCCATA 
CACAGCGACG CCTACTTCCT GTCCAGCCAC CTCATCATTG ACTACTCCCT GCTGGTGGGG 
TGGGACGATG CCACCGACCA GCTGGTGGTT GGGATCATAG ACTATATTCG GACGTTTACC 
TGGGACAAGA AATTAGAGAT GGTTGTCAAA TCCACTGGAA TCCTGGGGGG TCAGGGAAAG 
ATGCCCACCG TTGTTTCTCC TGAGCTGTAC CGAGCTCGGT TCTGTGAGGC CATGGACAAA 
TACTTCCTGA TGGTACCCGA CCACTGGACG GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605835.1
CDS	508..6318
Translation	MEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607533.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607533.2

ORF Insert Sequence:

ATGGAGGGAA AGAGCCAGGA CAGTGACTTG AAGCAGTACT GGATGCCGGA CAGCCAGTGT 
AAAGAATGTT ATGACTGCAA TGAAAAGTTC ACAACCTTCC GACGCCGCCA CCATTGCCGA 
CTGTGTGGTC AGATTTTCTG CAGTCGCTGC TGCAACCAGG AAATCCCTGG AAAGTTCATG 
GGTTACACAG GAGATTTGCG GGCCTGTACG TACTGCCGTA AGTTGGCACT GAGCTACGCC 
CACTCGGCTG ATTCGGGCTC CATCGGCGAA GACTTGAACA CCCTGACTGA CTCTCCGAGC 
TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA CGGACGCCTG TGGGGGGGCG CAAGGCCAGC 
AGGAACATCT TCCTAGAGGA AGACTTAACA TGGCAGAGAA AAACACCTAT TGGGATGAGA 
AAGAACATGA TTCACCAGGA ACCTCAGAGC AGCGGCCTCA GTTCCAGACT GACAGGACTT 
CAGGAGGATG TAGGAAAATC ACCTGGTAGG AGGAGGTCTG CTAGCATCAG CACACTTCCA 
CTTGACCGCT CTGGGTCATC AATGGTACCT TCCTACGACA GCTCCGTCAG CCCCCCTACC 
AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG ACAAAATTAG ATCACAGTGA GGAGGAACGT 
AAAATACTAC TGGACTCCTC CCAGTTGAAG GACTTATGGA GGAAAATCTG TCATAATGGA 
ACAGGGATGG AATTCCAGGA CCACAGATAC TGGCTGAGAA CCTACCCCAA CTGCATTGTG 
GGAAAAGAGC TCGTCAACTG GCTGCTAAGA AATGGCACCA TTTCCAACAG GGTCCAAGCT 
ATTGCCATAG GTCAGGCACT CGTGGATGGC CGCTGGCTCG ACTGTGTCAC CCACCACGAT 
CAGCTATTCA GAGATGAGTA TGCTCTCTAT CGACCACTTC AGAGTACAGA GTTCTCAGAA 
ACTCCCTCCC CGGACAGCGA TAGTGTCAAC TCACTAGAGG GACATTCAGA GCCGTCATGG 
TTCAAGGACA TCAAATTCGA TGACAGCGAC ACCGAGCAGC CCGCAGACGA CACAGAGTAC 
ACCATGCCTA GTTCCGCTGG GCCCAGCAAG AGGACATCTG TCAGCAGCGT CCACTCAGCA 
GTAGACAGTG ACTCAGCTGC CTCCATCAAC CTCAACATGG AGCAAAACAA TGTGAATTTT 
CGAATCAAGA AAGATTCCAA GTGTCCCCAG GCATTCCCCG CCCTGGAGCA CAAATCTGAA 
ATTATGGCGT CTGAAGATGG AGGAGAGAAC ATCCTTATAA GTGATGCCTT TATCAAAGAG 
TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG GCTAAAGAAA TGCTGTTTAC TCCACTGGGT 
TGGCACCACA GCTCTCTGGA CCAGCTCAGA GAGGAGAATG GAGAAAAGAA GGCTATGGAG 
AGGCTGCTCT CGGCAAATCA CAGCCACATG ATGGCGCTGC TGCAGCAGTT GCTCTACAGC 
GAGTCTTTGT CTCTGTCCTG GCGGGATATT ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG 
ACAGTTCGAC CTGATGTTCG CAACAGTGAT GACGACATGG ACATCCGCCA ACTGGTCCAT 
ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT GACTCAACTG TAGTGAATGG CTTTGTGTGT 
ACGAAGAGTA TTGCCCACAA AAAGATGAAC CCATATATCA AGAACCCCAA AATTCTGCTG 
CTGAAATGCT CAATAGAGTA TCTGTACAGG GAAGAGACCA AATTCACGTC CATCGACCCC 
ATCGTGCTGC AGGAACGAGA GTTTCTGAAA AACTATGTGC AACGTATCGT GGATGTTCGG 
CCCACTCTGG TTCTGGTGGA AAAAACGGTG TCTCGTATAG CTCAGGACAT GCTGCTAGAA 
CACGGCATTA CCCTGGTCAT TAATGTCAAA CCGCAAGTAT TGGACAGGGT GAGTTGTGTG 
ACCCAGGGGG ACCTTGTCAT GTCCATGGAC CAGCTACTCA CCAAACCTCG TCTGGGTACC 
TGCCACAAGT TTTACATGCA GCCCTTCCCT TTGGCCAATA ATGAGGTCAA GACTCTGATG 
TTCTTCGAAG GATGTCCTGC TCATCTCGGG TGCTCCATAA ATCTACGTGG TGCTTCAGAC 
TACGAGTTAG CCAGGGTGAA GGAGATCATG CGATTAATGG TGTGTGTGGC CTACCACTCT 
CAGCTGGAGA TCTCCTTCCT CATGGATGAG TTTGCCATGC CGCCCAGCTT GTCCCAGAGC 
ACTTCATTTC CCTGTCTCCT CGACGGAGGC TCTGCAGATG AGGAAGCAAG CAGGGAGAAA 
CTAGAGAAGA GTTCTGAAGA TGAAGACACT GTAGTGGAAG GACAACCTGA GGAGGTGCCC 
TCTGCCAATG ATGTAGACAC TTCCCAGGAC ACAAATGATC CTAAATTATT CTCCTCTATG 
GTGGAAGAGA CCACTAATAC TGACACAGTC ACGACAAAGT CTTCCACTCC GTTCCAAAGC 
CCTCGTGCGC CATGCTTGCC AGTTCCTCCA CCATTCATCA TGGAAGAAAC CCCAGAAATG 
GGCTCAGACA GCCTAACAGG GGCATTTGAG GCAGGGGTGG TGGAATGCTG CCCAAAGGAG 
GAATCAACCA ACCTAGAAGA TGGAGAAGAT TCTGATATTC CTACTCCTAA GTTGTTCCGA 
GACCCCCTGC AAGATGATAC TGGGATGTTT GTGGCTGAAG AAGTTGCTTC TTCAGATGAC 
CATCTCAAGT CCATCTCTGC TATCTTCAAA CAGGAACTTA AGGACATCAT CCTGTGCATT 
TCACCTTTCA TCACATTTGA GAAACCATTT CTTCTTACCC CTGCTGGGAT GCACTGCCCG 
AGCAGGGATT ATTTTCCCGA ACAGGTCTAT CTGTCTCCTC TCCTCAACAA GGACTTTAAA 
GAGTTGGATG GACGCAGAAA GCGGCAGCTC CTTAAGGAGT CCACTCCCTC CCTGGCAAAC 
AGCCTAACGA ATGGTGGCCC CCCGTCAAAG CATGTCAGTG TCCTGCCATC CCACAGTCTC 
ACCAGCACCC GGATCGTTGA ACTAGTAAAC AGGAGCCAGG GTCTGGCCAG AATGGTTGCA 
GACTACAGAG CAAAGGGTGG ACGTATCCAG CACAGCGAAG CTACTGATCC ATTTTGCATA 
AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG AATAGACCAG GTGATGGTTC GGTCAGAGCA 
CCAGTAAAGA CTGACAGCGA AGAGGAGAAG CCAACTAAAC CCAGTGACTG GACCCCCAAG 
CTGGATTGTC TGAACCCCAC CAACCACCAG AGACTCTGTG TGCTTTTTAG CAGTTCATCA 
ACTCAGTCCA GCAATGCCCC CAACCCCTGT GTCAACCCAT GGATTGTTAC CATGGAGTTT 
TATGGCAAGA ATGACCTCTG CCTTGGTGTA TTCTTGGAGC GATATTGCTT TAGGCCATCA 
TACCAGTGCC CCAGTATATT TTGTGAGACC CCCATGGTGC ACCACATCCG GCGTTTTGTC 
CATGGCAACG GCTGCGTGCA GATAGTACTG AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC 
CAGCACACCA TTCTCAACTA CTCCTGGTGC CGCATCTGCA AACAAGTGAC ACCAGTGGTT 
CCTCTATCCA ATGACTCATG GTCAATGTCT TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC 
GGCCACCAGT ACACCAGGAG GGCCAATGCA GAGCCCTGTG GCCACTCCAT CCACAAAGAC 
TACCACCAGT ACTTCTCATA CAACCAAATG GTGGCTTCCT TCAGTTACAC CTCAGTGAGG 
CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA ATCTTCATCA GGAATCAGGG TCCTTCGATC 
AACAACTTGG GGCAGGATCT GAAAGACTTC TCTCAAAAAG TAAATCAGGT TTACGTGGCC 
ATAGATGATC GTCTCACATC TCTAAAGACG GACACCTTCA GTAAAACACG CGAGGAGAAG 
ATGGAGGACC TCTTTGCACA GAAAGATATG GAAGAGGCGG AACTGCGCAG CTGGATTGAA 
AAGTTTCAAG CACGATTGCA GGCTTGTGGT CACGTTTCTC CTCAGCAGCT GCAGACGACT 
CTGGAATCAC TGGTGATGAA GAAACAGAGT CTGTGTGAGA TGTTGCAGTA CTGGAACAGC 
AGGCTGCAGG AATTATTCCA ACAGGAGAAA GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC 
CCCGGCAGGC ACAGACAGAC TGCCCCTGAT GATGGCAAGA ACGCTGTGGA GTCATCCCCA 
CGTAACCCTT CACCTGTGGT ACAGAATGGT GAAAAGGAGG ACCGTCACCT CACCACACTG 
CCTTCAACCT CCTCTCTGTT GCCCTCGCCT GGAGACCTGG GAGCTGAACC AGCCACATCT 
GGTCCTTCCC TGAGTGAGCA GGACTCTGTC AGCATCCCAG AAGATGTATT TGATGGACAC 
TTGCTGGGCT CCAATGACAG TCAGGTGAAG GAGAAATCCA CCATGAAAGC CATCCTTGCC 
AACTTCCTGC CCGGCAACAG TTACAACCCC ATTCCATTCC CATTTGACCC AGACAAACAC 
TACCTGATGG ATGAGTATGA GCGAGTGCCA ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC 
ATCATTGCAT TTGCTCTGAG TTGTAAGAAG TATAAAACAG CACTGGAAGA TCTGTCCAAG 
GTGTCAAACA TGGGTGGAGA AGACTCTTCA CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG 
AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA GCCTCGTCCC AACAGAGCCG CAGCAGCAGT 
GAAGCAGATC CCCTCAGTAG GCGTCCAGAA GACGCAGACC TAACAGACAA ACAGAAGAAG 
CAGGCCCTGA ATCCCCACAT TGAGCTACAG TTCTCTGATG CAAGCGCAAA GTTTTACTGT 
CGGATCTACT ACGCCGAGGA GTTTCATAAG ATGCGTGAGG AGATCATGGA GAGCTCCGAA 
GAGGACTTTG TGCGTTCCCT GTCGCACTGT GTCAACTGGC AGGCGCGCGG TGGAAAGTCT 
GGGGCCGTTT TCTACGCAAC AGAAGACGAC CGTTTCATCC TAAAACAGAT GCCCAGGCTG 
GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG CACTACTTCA CCTACATCAC CGGAGCTGTG 
CAGCAGAAGA GGCCAACTGC ATTGGCAAAG ATTCTCGGAG TTTACAGGAT AGGCTACAAG 
AACTCCCAGA ACAACACTGA GAAGAAACTG GACCTGCTCG TCATGGAAAA CCTGTTCTAC 
GGACGCAAGA TGGCTCAGGT CTTTGACCTG AAAGGTTCCC TCAGAAACCG TCATGTGAAG 
ACAGACCTGG TAAAGGAAAG CTGCGAGGTG GTCCTGCTGG ATGAGAATCT ACTGAAGCTG 
ATCCACGACA ATCCGCTCTA CATTCGCTCT CACTGCAAGT CCATCTTGCG CGCCGCCATA 
CACAGCGACG CCTACTTCCT GTCCAGCCAC CTCATCATTG ACTACTCCCT GCTGGTGGGG 
TGGGACGATG CCACCGACCA GCTGGTGGTT GGGATCATAG ACTATATTCG GACGTTTACC 
TGGGACAAGA AATTAGAGAT GGTTGTCAAA TCCACTGGAA TCCTGGGGGG TCAGGGAAAG 
ATGCCCACCG TTGTTTCTCC TGAGCTGTAC CGAGCTCGGT TCTGTGAGGC CATGGACAAA 
TACTTCCTGA TGGTACCCGA CCACTGGACG GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607533.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605835.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.

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CloneID	OTo31331
Clone ID Related Accession (Same CDS sequence)	XM_011607533.1
Accession Version	XM_011607533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGAA AGAGCCAGGA CAGTGACTTG AAGCAGTACT GGATGCCGGA CAGCCAGTGT 
AAAGAATGTT ATGACTGCAA TGAAAAGTTC ACAACCTTCC GACGCCGCCA CCATTGCCGA 
CTGTGTGGTC AGATTTTCTG CAGTCGCTGC TGCAACCAGG AAATCCCTGG AAAGTTCATG 
GGTTACACAG GAGATTTGCG GGCCTGTACG TACTGCCGTA AGTTGGCACT GAGCTACGCC 
CACTCGGCTG ATTCGGGCTC CATCGGCGAA GACTTGAACA CCCTGACTGA CTCTCCGAGC 
TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA CGGACGCCTG TGGGGGGGCG CAAGGCCAGC 
AGGAACATCT TCCTAGAGGA AGACTTAACA TGGCAGAGAA AAACACCTAT TGGGATGAGA 
AAGAACATGA TTCACCAGGA ACCTCAGAGC AGCGGCCTCA GTTCCAGACT GACAGGACTT 
CAGGAGGATG TAGGAAAATC ACCTGGTAGG AGGAGGTCTG CTAGCATCAG CACACTTCCA 
CTTGACCGCT CTGGGTCATC AATGGTACCT TCCTACGACA GCTCCGTCAG CCCCCCTACC 
AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG ACAAAATTAG ATCACAGTGA GGAGGAACGT 
AAAATACTAC TGGACTCCTC CCAGTTGAAG GACTTATGGA GGAAAATCTG TCATAATGGA 
ACAGGGATGG AATTCCAGGA CCACAGATAC TGGCTGAGAA CCTACCCCAA CTGCATTGTG 
GGAAAAGAGC TCGTCAACTG GCTGCTAAGA AATGGCACCA TTTCCAACAG GGTCCAAGCT 
ATTGCCATAG GTCAGGCACT CGTGGATGGC CGCTGGCTCG ACTGTGTCAC CCACCACGAT 
CAGCTATTCA GAGATGAGTA TGCTCTCTAT CGACCACTTC AGAGTACAGA GTTCTCAGAA 
ACTCCCTCCC CGGACAGCGA TAGTGTCAAC TCACTAGAGG GACATTCAGA GCCGTCATGG 
TTCAAGGACA TCAAATTCGA TGACAGCGAC ACCGAGCAGC CCGCAGACGA CACAGAGTAC 
ACCATGCCTA GTTCCGCTGG GCCCAGCAAG AGGACATCTG TCAGCAGCGT CCACTCAGCA 
GTAGACAGTG ACTCAGCTGC CTCCATCAAC CTCAACATGG AGCAAAACAA TGTGAATTTT 
CGAATCAAGA AAGATTCCAA GTGTCCCCAG GCATTCCCCG CCCTGGAGCA CAAATCTGAA 
ATTATGGCGT CTGAAGATGG AGGAGAGAAC ATCCTTATAA GTGATGCCTT TATCAAAGAG 
TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG GCTAAAGAAA TGCTGTTTAC TCCACTGGGT 
TGGCACCACA GCTCTCTGGA CCAGCTCAGA GAGGAGAATG GAGAAAAGAA GGCTATGGAG 
AGGCTGCTCT CGGCAAATCA CAGCCACATG ATGGCGCTGC TGCAGCAGTT GCTCTACAGC 
GAGTCTTTGT CTCTGTCCTG GCGGGATATT ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG 
ACAGTTCGAC CTGATGTTCG CAACAGTGAT GACGACATGG ACATCCGCCA ACTGGTCCAT 
ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT GACTCAACTG TAGTGAATGG CTTTGTGTGT 
ACGAAGAGTA TTGCCCACAA AAAGATGAAC CCATATATCA AGAACCCCAA AATTCTGCTG 
CTGAAATGCT CAATAGAGTA TCTGTACAGG GAAGAGACCA AATTCACGTC CATCGACCCC 
ATCGTGCTGC AGGAACGAGA GTTTCTGAAA AACTATGTGC AACGTATCGT GGATGTTCGG 
CCCACTCTGG TTCTGGTGGA AAAAACGGTG TCTCGTATAG CTCAGGACAT GCTGCTAGAA 
CACGGCATTA CCCTGGTCAT TAATGTCAAA CCGCAAGTAT TGGACAGGGT GAGTTGTGTG 
ACCCAGGGGG ACCTTGTCAT GTCCATGGAC CAGCTACTCA CCAAACCTCG TCTGGGTACC 
TGCCACAAGT TTTACATGCA GCCCTTCCCT TTGGCCAATA ATGAGGTCAA GACTCTGATG 
TTCTTCGAAG GATGTCCTGC TCATCTCGGG TGCTCCATAA ATCTACGTGG TGCTTCAGAC 
TACGAGTTAG CCAGGGTGAA GGAGATCATG CGATTAATGG TGTGTGTGGC CTACCACTCT 
CAGCTGGAGA TCTCCTTCCT CATGGATGAG TTTGCCATGC CGCCCAGCTT GTCCCAGAGC 
ACTTCATTTC CCTGTCTCCT CGACGGAGGC TCTGCAGATG AGGAAGCAAG CAGGGAGAAA 
CTAGAGAAGA GTTCTGAAGA TGAAGACACT GTAGTGGAAG GACAACCTGA GGAGGTGCCC 
TCTGCCAATG ATGTAGACAC TTCCCAGGAC ACAAATGATC CTAAATTATT CTCCTCTATG 
GTGGAAGAGA CCACTAATAC TGACACAGTC ACGACAAAGT CTTCCACTCC GTTCCAAAGC 
CCTCGTGCGC CATGCTTGCC AGTTCCTCCA CCATTCATCA TGGAAGAAAC CCCAGAAATG 
GGCTCAGACA GCCTAACAGG GGCATTTGAG GCAGGGGTGG TGGAATGCTG CCCAAAGGAG 
GAATCAACCA ACCTAGAAGA TGGAGAAGAT TCTGATATTC CTACTCCTAA ATTGTTCCGA 
GACCCCCTGC AAGATGATAC TGGGATGTTT GTGGCTGAAG AAGTTGCTTC TTCAGATGAC 
CATCTCAAGT CCATCTCTGC TATCTTCAAA CAGGAACTTA AGGACATCAT CCTGTGCATT 
TCACCTTTCA TCACATTTGA GAAACCATTT CTTCTTACCC CTGCTGGGAT GCACTGCCCG 
AGCAGGGATT ATTTTCCCGA ACAGGTCTAT CTGTCTCCTC TCCTCAACAA GGACTTTAAA 
GAGTTGGATG GACGCAGAAA GCGGCAGCTC CTTAAGGAGT CCACTCCCTC CCTGGCAAAC 
AGCCTAACGA ATGGTGGCCC CCCGTCAAAG CATGTCAGTG TCCTGCCATC CCACAGTCTC 
ACCAGCACCC GGATCGTTGA ACTAGTAAAC AGGAGCCAGG GTCTGGCCAG AATGGTTGCA 
GACTACAGAG CAAAGGGTGG ACGTATCCAG CACAGCGAAG CTACTGATCC ATTTTGCATA 
AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG AATAGACCAG GTGATGGTTC GGTCAGAGCA 
CCAGTAAAGA CTGACAGCGA AGAGGAGAAG CCAACTAAAC CCAGTGACTG GACCCCCAAG 
CTGGATTGTC TGAACCCCAC CAACCACCAG AGACTCTGTG TGCTTTTTAG CAGTTCATCA 
ACTCAGTCCA GCAATGCCCC CAACCCCTGT GTCAACCCAT GGATTGTTAC CATGGAGTTT 
TATGGCAAGA ATGACCTCTG CCTTGGTGTA TTCTTGGAGC GATATTGCTT TAGGCCATCA 
TACCAGTGCC CCAGTATATT TTGTGAGACC CCCATGGTGC ACCACATCCG GCGTTTTGTC 
CATGGCAACG GCTGCGTGCA GATAGTACTG AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC 
CAGCACACCA TTCTCAACTA CTCCTGGTGC CGCATCTGCA AACAAGTGAC ACCAGTGGTT 
CCTCTATCCA ATGACTCATG GTCAATGTCT TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC 
GGCCACCAGT ACACCAGGAG GGCCAATGCA GAGCCCTGTG GCCACTCCAT CCACAAAGAC 
TACCACCAGT ACTTCTCATA CAACCAAATG GTGGCTTCCT TCAGTTACAC CTCAGTGAGG 
CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA ATCTTCATCA GGAATCAGGG TCCTTCGATC 
AACAACTTGG GGCAGGATCT GAAAGACTTC TCTCAAAAAG TAAATCAGGT TTACGTGGCC 
ATAGATGATC GTCTCACATC TCTAAAGACG GACACCTTCA GTAAAACACG CGAGGAGAAG 
ATGGAGGACC TCTTTGCACA GAAAGATATG GAAGAGGCGG AACTGCGCAG CTGGATTGAA 
AAGTTTCAAG CACGATTGCA GGCTTGTGGT CACGTTTCTC CTCAGCAGCT GCAGACGACT 
CTGGAATCAC TGGTGATGAA GAAACAGAGT CTGTGTGAGA TGTTGCAGTA CTGGAACAGC 
AGGCTGCAGG AATTATTCCA ACAGGAGAAA GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC 
CCCGGCAGGC ACAGACAGAC TGCCCCTGAT GATGGCAAGA ACGCTGTGGA GTCATCCCCA 
CGTAACCCTT CACCTGTGGT ACAGAATGGT GAAAAGGAGG ACCGTCACCT CACCACACTG 
CCTTCAACCT CCTCTCTGTT GCCCTCGCCT GGAGACCTGG GAGCTGAACC AGCCACATCT 
GGTCCTTCCC TGAGTGAGCA GGACTCTGTC AGCATCCCAG AAGATGTATT TGATGGACAC 
TTGCTGGGCT CCAATGACAG TCAGGTGAAG GAGAAATCCA CCATGAAAGC CATCCTTGCC 
AACTTCCTGC CCGGCAACAG TTACAACCCC ATTCCATTCC CATTTGACCC AGACAAACAC 
TACCTGATGG ATGAGTATGA GCGAGTGCCA ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC 
ATCATTGCAT TTGCTCTGAG TTGTAAGAAG TATAAAACAG CACTGGAAGA TCTGTCCAAG 
GTGTCAAACA TGGGTGGAGA AGACTCTTCA CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG 
AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA GCCTCGTCCC AACAGAGCCG CAGCAGCAGT 
GAAGCAGATC CCCTCAGTAG GCGTCCAGAA GACGCAGACC TAACAGACAA ACAGAAGAAG 
CAGGCCCTGA ATCCCCACAT TGAGCTACAG TTCTCTGATG CAAGCGCAAA GTTTTACTGT 
CGGATCTACT ACGCCGAGGA GTTTCATAAG ATGCGTGAGG AGATCATGGA GAGCTCCGAA 
GAGGACTTTG TGCGTTCCCT GTCGCACTGT GTCAACTGGC AGGCGCGCGG TGGAAAGTCT 
GGGGCCGTTT TCTACGCAAC AGAAGACGAC CGTTTCATCC TAAAACAGAT GCCCAGGCTG 
GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG CACTACTTCA CCTACATCAC CGGAGCTGTG 
CAGCAGAAGA GGCCAACTGC ATTGGCAAAG ATTCTCGGAG TTTACAGGAT AGGCTACAAG 
AACTCCCAGA ACAACACTGA GAAGAAACTG GACCTGCTCG TCATGGAAAA CCTGTTCTAC 
GGACGCAAGA TGGCTCAGGT CTTTGACCTG AAAGGTTCCC TCAGAAACCG TCATGTGAAG 
ACAGACCTGG TAAAGGAAAG CTGCGAGGTG GTCCTGCTGG ATGAGAATCT ACTGAAGCTG 
ATCCACGACA ATCCGCTCTA CATTCGCTCT CACTGCAAGT CCATCTTGCG CGCCGCCATA 
CACAGCGACG CCTACTTCCT GTCCAGCCAC CTCATCATTG ACTACTCCCT GCTGGTGGGG 
TGGGACGATG CCACCGACCA GCTGGTGGTT GGGATCATAG ACTATATTCG GACGTTTACC 
TGGGACAAGA AATTAGAGAT GGTTGTCAAA TCCACTGGAA TCCTGGGGGG TCAGGGAAAG 
ATGCCCACCG TTGTTTCTCC TGAGCTGTAC CGAGCTCGGT TCTGTGAGGC CATGGACAAA 
TACTTCCTGA TGGTACCCGA CCACTGGACG GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605835.1
CDS	461..6271
Translation	MEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607533.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607533.1

ORF Insert Sequence:

ATGGAGGGAA AGAGCCAGGA CAGTGACTTG AAGCAGTACT GGATGCCGGA CAGCCAGTGT 
AAAGAATGTT ATGACTGCAA TGAAAAGTTC ACAACCTTCC GACGCCGCCA CCATTGCCGA 
CTGTGTGGTC AGATTTTCTG CAGTCGCTGC TGCAACCAGG AAATCCCTGG AAAGTTCATG 
GGTTACACAG GAGATTTGCG GGCCTGTACG TACTGCCGTA AGTTGGCACT GAGCTACGCC 
CACTCGGCTG ATTCGGGCTC CATCGGCGAA GACTTGAACA CCCTGACTGA CTCTCCGAGC 
TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA CGGACGCCTG TGGGGGGGCG CAAGGCCAGC 
AGGAACATCT TCCTAGAGGA AGACTTAACA TGGCAGAGAA AAACACCTAT TGGGATGAGA 
AAGAACATGA TTCACCAGGA ACCTCAGAGC AGCGGCCTCA GTTCCAGACT GACAGGACTT 
CAGGAGGATG TAGGAAAATC ACCTGGTAGG AGGAGGTCTG CTAGCATCAG CACACTTCCA 
CTTGACCGCT CTGGGTCATC AATGGTACCT TCCTACGACA GCTCCGTCAG CCCCCCTACC 
AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG ACAAAATTAG ATCACAGTGA GGAGGAACGT 
AAAATACTAC TGGACTCCTC CCAGTTGAAG GACTTATGGA GGAAAATCTG TCATAATGGA 
ACAGGGATGG AATTCCAGGA CCACAGATAC TGGCTGAGAA CCTACCCCAA CTGCATTGTG 
GGAAAAGAGC TCGTCAACTG GCTGCTAAGA AATGGCACCA TTTCCAACAG GGTCCAAGCT 
ATTGCCATAG GTCAGGCACT CGTGGATGGC CGCTGGCTCG ACTGTGTCAC CCACCACGAT 
CAGCTATTCA GAGATGAGTA TGCTCTCTAT CGACCACTTC AGAGTACAGA GTTCTCAGAA 
ACTCCCTCCC CGGACAGCGA TAGTGTCAAC TCACTAGAGG GACATTCAGA GCCGTCATGG 
TTCAAGGACA TCAAATTCGA TGACAGCGAC ACCGAGCAGC CCGCAGACGA CACAGAGTAC 
ACCATGCCTA GTTCCGCTGG GCCCAGCAAG AGGACATCTG TCAGCAGCGT CCACTCAGCA 
GTAGACAGTG ACTCAGCTGC CTCCATCAAC CTCAACATGG AGCAAAACAA TGTGAATTTT 
CGAATCAAGA AAGATTCCAA GTGTCCCCAG GCATTCCCCG CCCTGGAGCA CAAATCTGAA 
ATTATGGCGT CTGAAGATGG AGGAGAGAAC ATCCTTATAA GTGATGCCTT TATCAAAGAG 
TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG GCTAAAGAAA TGCTGTTTAC TCCACTGGGT 
TGGCACCACA GCTCTCTGGA CCAGCTCAGA GAGGAGAATG GAGAAAAGAA GGCTATGGAG 
AGGCTGCTCT CGGCAAATCA CAGCCACATG ATGGCGCTGC TGCAGCAGTT GCTCTACAGC 
GAGTCTTTGT CTCTGTCCTG GCGGGATATT ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG 
ACAGTTCGAC CTGATGTTCG CAACAGTGAT GACGACATGG ACATCCGCCA ACTGGTCCAT 
ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT GACTCAACTG TAGTGAATGG CTTTGTGTGT 
ACGAAGAGTA TTGCCCACAA AAAGATGAAC CCATATATCA AGAACCCCAA AATTCTGCTG 
CTGAAATGCT CAATAGAGTA TCTGTACAGG GAAGAGACCA AATTCACGTC CATCGACCCC 
ATCGTGCTGC AGGAACGAGA GTTTCTGAAA AACTATGTGC AACGTATCGT GGATGTTCGG 
CCCACTCTGG TTCTGGTGGA AAAAACGGTG TCTCGTATAG CTCAGGACAT GCTGCTAGAA 
CACGGCATTA CCCTGGTCAT TAATGTCAAA CCGCAAGTAT TGGACAGGGT GAGTTGTGTG 
ACCCAGGGGG ACCTTGTCAT GTCCATGGAC CAGCTACTCA CCAAACCTCG TCTGGGTACC 
TGCCACAAGT TTTACATGCA GCCCTTCCCT TTGGCCAATA ATGAGGTCAA GACTCTGATG 
TTCTTCGAAG GATGTCCTGC TCATCTCGGG TGCTCCATAA ATCTACGTGG TGCTTCAGAC 
TACGAGTTAG CCAGGGTGAA GGAGATCATG CGATTAATGG TGTGTGTGGC CTACCACTCT 
CAGCTGGAGA TCTCCTTCCT CATGGATGAG TTTGCCATGC CGCCCAGCTT GTCCCAGAGC 
ACTTCATTTC CCTGTCTCCT CGACGGAGGC TCTGCAGATG AGGAAGCAAG CAGGGAGAAA 
CTAGAGAAGA GTTCTGAAGA TGAAGACACT GTAGTGGAAG GACAACCTGA GGAGGTGCCC 
TCTGCCAATG ATGTAGACAC TTCCCAGGAC ACAAATGATC CTAAATTATT CTCCTCTATG 
GTGGAAGAGA CCACTAATAC TGACACAGTC ACGACAAAGT CTTCCACTCC GTTCCAAAGC 
CCTCGTGCGC CATGCTTGCC AGTTCCTCCA CCATTCATCA TGGAAGAAAC CCCAGAAATG 
GGCTCAGACA GCCTAACAGG GGCATTTGAG GCAGGGGTGG TGGAATGCTG CCCAAAGGAG 
GAATCAACCA ACCTAGAAGA TGGAGAAGAT TCTGATATTC CTACTCCTAA ATTGTTCCGA 
GACCCCCTGC AAGATGATAC TGGGATGTTT GTGGCTGAAG AAGTTGCTTC TTCAGATGAC 
CATCTCAAGT CCATCTCTGC TATCTTCAAA CAGGAACTTA AGGACATCAT CCTGTGCATT 
TCACCTTTCA TCACATTTGA GAAACCATTT CTTCTTACCC CTGCTGGGAT GCACTGCCCG 
AGCAGGGATT ATTTTCCCGA ACAGGTCTAT CTGTCTCCTC TCCTCAACAA GGACTTTAAA 
GAGTTGGATG GACGCAGAAA GCGGCAGCTC CTTAAGGAGT CCACTCCCTC CCTGGCAAAC 
AGCCTAACGA ATGGTGGCCC CCCGTCAAAG CATGTCAGTG TCCTGCCATC CCACAGTCTC 
ACCAGCACCC GGATCGTTGA ACTAGTAAAC AGGAGCCAGG GTCTGGCCAG AATGGTTGCA 
GACTACAGAG CAAAGGGTGG ACGTATCCAG CACAGCGAAG CTACTGATCC ATTTTGCATA 
AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG AATAGACCAG GTGATGGTTC GGTCAGAGCA 
CCAGTAAAGA CTGACAGCGA AGAGGAGAAG CCAACTAAAC CCAGTGACTG GACCCCCAAG 
CTGGATTGTC TGAACCCCAC CAACCACCAG AGACTCTGTG TGCTTTTTAG CAGTTCATCA 
ACTCAGTCCA GCAATGCCCC CAACCCCTGT GTCAACCCAT GGATTGTTAC CATGGAGTTT 
TATGGCAAGA ATGACCTCTG CCTTGGTGTA TTCTTGGAGC GATATTGCTT TAGGCCATCA 
TACCAGTGCC CCAGTATATT TTGTGAGACC CCCATGGTGC ACCACATCCG GCGTTTTGTC 
CATGGCAACG GCTGCGTGCA GATAGTACTG AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC 
CAGCACACCA TTCTCAACTA CTCCTGGTGC CGCATCTGCA AACAAGTGAC ACCAGTGGTT 
CCTCTATCCA ATGACTCATG GTCAATGTCT TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC 
GGCCACCAGT ACACCAGGAG GGCCAATGCA GAGCCCTGTG GCCACTCCAT CCACAAAGAC 
TACCACCAGT ACTTCTCATA CAACCAAATG GTGGCTTCCT TCAGTTACAC CTCAGTGAGG 
CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA ATCTTCATCA GGAATCAGGG TCCTTCGATC 
AACAACTTGG GGCAGGATCT GAAAGACTTC TCTCAAAAAG TAAATCAGGT TTACGTGGCC 
ATAGATGATC GTCTCACATC TCTAAAGACG GACACCTTCA GTAAAACACG CGAGGAGAAG 
ATGGAGGACC TCTTTGCACA GAAAGATATG GAAGAGGCGG AACTGCGCAG CTGGATTGAA 
AAGTTTCAAG CACGATTGCA GGCTTGTGGT CACGTTTCTC CTCAGCAGCT GCAGACGACT 
CTGGAATCAC TGGTGATGAA GAAACAGAGT CTGTGTGAGA TGTTGCAGTA CTGGAACAGC 
AGGCTGCAGG AATTATTCCA ACAGGAGAAA GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC 
CCCGGCAGGC ACAGACAGAC TGCCCCTGAT GATGGCAAGA ACGCTGTGGA GTCATCCCCA 
CGTAACCCTT CACCTGTGGT ACAGAATGGT GAAAAGGAGG ACCGTCACCT CACCACACTG 
CCTTCAACCT CCTCTCTGTT GCCCTCGCCT GGAGACCTGG GAGCTGAACC AGCCACATCT 
GGTCCTTCCC TGAGTGAGCA GGACTCTGTC AGCATCCCAG AAGATGTATT TGATGGACAC 
TTGCTGGGCT CCAATGACAG TCAGGTGAAG GAGAAATCCA CCATGAAAGC CATCCTTGCC 
AACTTCCTGC CCGGCAACAG TTACAACCCC ATTCCATTCC CATTTGACCC AGACAAACAC 
TACCTGATGG ATGAGTATGA GCGAGTGCCA ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC 
ATCATTGCAT TTGCTCTGAG TTGTAAGAAG TATAAAACAG CACTGGAAGA TCTGTCCAAG 
GTGTCAAACA TGGGTGGAGA AGACTCTTCA CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG 
AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA GCCTCGTCCC AACAGAGCCG CAGCAGCAGT 
GAAGCAGATC CCCTCAGTAG GCGTCCAGAA GACGCAGACC TAACAGACAA ACAGAAGAAG 
CAGGCCCTGA ATCCCCACAT TGAGCTACAG TTCTCTGATG CAAGCGCAAA GTTTTACTGT 
CGGATCTACT ACGCCGAGGA GTTTCATAAG ATGCGTGAGG AGATCATGGA GAGCTCCGAA 
GAGGACTTTG TGCGTTCCCT GTCGCACTGT GTCAACTGGC AGGCGCGCGG TGGAAAGTCT 
GGGGCCGTTT TCTACGCAAC AGAAGACGAC CGTTTCATCC TAAAACAGAT GCCCAGGCTG 
GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG CACTACTTCA CCTACATCAC CGGAGCTGTG 
CAGCAGAAGA GGCCAACTGC ATTGGCAAAG ATTCTCGGAG TTTACAGGAT AGGCTACAAG 
AACTCCCAGA ACAACACTGA GAAGAAACTG GACCTGCTCG TCATGGAAAA CCTGTTCTAC 
GGACGCAAGA TGGCTCAGGT CTTTGACCTG AAAGGTTCCC TCAGAAACCG TCATGTGAAG 
ACAGACCTGG TAAAGGAAAG CTGCGAGGTG GTCCTGCTGG ATGAGAATCT ACTGAAGCTG 
ATCCACGACA ATCCGCTCTA CATTCGCTCT CACTGCAAGT CCATCTTGCG CGCCGCCATA 
CACAGCGACG CCTACTTCCT GTCCAGCCAC CTCATCATTG ACTACTCCCT GCTGGTGGGG 
TGGGACGATG CCACCGACCA GCTGGTGGTT GGGATCATAG ACTATATTCG GACGTTTACC 
TGGGACAAGA AATTAGAGAT GGTTGTCAAA TCCACTGGAA TCCTGGGGGG TCAGGGAAAG 
ATGCCCACCG TTGTTTCTCC TGAGCTGTAC CGAGCTCGGT TCTGTGAGGC CATGGACAAA 
TACTTCCTGA TGGTACCCGA CCACTGGACG GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832587.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688447.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X1, mRNA.

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CloneID	OTo40920
Clone ID Related Accession (Same CDS sequence)	XM_029832587.1
Accession Version	XM_029832587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA GTAGGCGTCC AGAAGACGCA GACCTAACAG ACAAACAGAA GAAGCAGGCC 
CTGAATCCCC ACATTGAGCT ACAGTTCTCT GATGCAAGCG CAAAGTTTTA CTGTCGGATC 
TACTACGCCG AGGAGTTTCA TAAGATGCGT GAGGAGATCA TGGAGAGCTC CGAAGAGGAC 
TTTGTGCGTT CCCTGTCGCA CTGTGTCAAC TGGCAGGCGC GCGGTGGAAA GTCTGGGGCC 
GTTTTCTACG CAACAGAAGA CGACCGTTTC ATCCTAAAAC AGATGCCCAG GCTGGAGGTG 
CAGTCCTTCT TGGACTTTGC TCCGCACTAC TTCACCTACA TCACCGGAGC TGTGCAGCAG 
AAGAGGCCAA CTGCATTGGC AAAGATTCTC GGAGTTTACA GGATAGGCTA CAAGAACTCC 
CAGAACAACA CTGAGAAGAA ACTGGACCTG CTCGTCATGG AAAACCTGTT CTACGGACGC 
AAGATGGCTC AGGTCTTTGA CCTGAAAGGT TCCCTCAGAA ACCGTCATGT GAAGACAGAC 
CTGGTAAAGG AAAGCTGCGA GGTGGTCCTG CTGGATGAGA ATCTACTGAA GCTGATCCAC 
GACAATCCGC TCTACATTCG CTCTCACTGC AAGTCCATCT TGCGCGCCGC CATACACAGC 
GACGCCTACT TCCTGTCCAG CCACCTCATC ATTGACTACT CCCTGCTGGT GGGGTGGGAC 
GATGCCACCG ACCAGCTGGT GGTTGGGATC ATAGACTATA TTCGGACGTT TACCTGGGAC 
AAGAAATTAG AGATGGTTGT CAAATCCACT GGAATCCTGG GGGGTCAGGG AAAGATGCCC 
ACCGTTGTTT CTCCTGAGCT GTACCGAGCT CGGTTCTGTG AGGCCATGGA CAAATACTTC 
CTGATGGTAC CCGACCACTG GACGGGTCTT GGCATCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688447.1
CDS	132..6476
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832587.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832587.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA GTAGGCGTCC AGAAGACGCA GACCTAACAG ACAAACAGAA GAAGCAGGCC 
CTGAATCCCC ACATTGAGCT ACAGTTCTCT GATGCAAGCG CAAAGTTTTA CTGTCGGATC 
TACTACGCCG AGGAGTTTCA TAAGATGCGT GAGGAGATCA TGGAGAGCTC CGAAGAGGAC 
TTTGTGCGTT CCCTGTCGCA CTGTGTCAAC TGGCAGGCGC GCGGTGGAAA GTCTGGGGCC 
GTTTTCTACG CAACAGAAGA CGACCGTTTC ATCCTAAAAC AGATGCCCAG GCTGGAGGTG 
CAGTCCTTCT TGGACTTTGC TCCGCACTAC TTCACCTACA TCACCGGAGC TGTGCAGCAG 
AAGAGGCCAA CTGCATTGGC AAAGATTCTC GGAGTTTACA GGATAGGCTA CAAGAACTCC 
CAGAACAACA CTGAGAAGAA ACTGGACCTG CTCGTCATGG AAAACCTGTT CTACGGACGC 
AAGATGGCTC AGGTCTTTGA CCTGAAAGGT TCCCTCAGAA ACCGTCATGT GAAGACAGAC 
CTGGTAAAGG AAAGCTGCGA GGTGGTCCTG CTGGATGAGA ATCTACTGAA GCTGATCCAC 
GACAATCCGC TCTACATTCG CTCTCACTGC AAGTCCATCT TGCGCGCCGC CATACACAGC 
GACGCCTACT TCCTGTCCAG CCACCTCATC ATTGACTACT CCCTGCTGGT GGGGTGGGAC 
GATGCCACCG ACCAGCTGGT GGTTGGGATC ATAGACTATA TTCGGACGTT TACCTGGGAC 
AAGAAATTAG AGATGGTTGT CAAATCCACT GGAATCCTGG GGGGTCAGGG AAAGATGCCC 
ACCGTTGTTT CTCCTGAGCT GTACCGAGCT CGGTTCTGTG AGGCCATGGA CAAATACTTC 
CTGATGGTAC CCGACCACTG GACGGGTCTT GGCATCAACT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832590.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688450.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X2, mRNA.

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CloneID	OTo40921
Clone ID Related Accession (Same CDS sequence)	XM_029832590.1
Accession Version	XM_029832590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGA CCAGCAGTGT CATGGCTGCT GATGATAAGT CATCCTCATC CTCAATGGAG 
TGGACCACTG AGACCCCCGT CACCTTGCCC GTGAGTCCGT CCCACCTGAC TCATTTTAAG 
CCACTGTCAC CCGAGCAGGA TGAGCCCCCG CTCCGTTCAG CATACAGCTC ATTTGTCAAC 
CTATTTCGCT TTAGTAACAA AGTCACAGGC AGTGACCACA GTCTTGTGAA GTCAGGTGAT 
AGAAAGGGCC TGTCTGGATT GATGCCAGGA ACCAAGGAGG GACGTTCTCT TTCAACAGTG 
TCAGAGAAAT CAGACACTCC ATCCCCTTCC CCTCTATCAG AGAGGAGGAG CTGGTCCTCC 
AGCCCACCCC ATTCCCTCTA CACTGCCAGG ATTCCCCGAA AACAACCAGA TCACCTTCAA 
AGGACCTCTG CTGCTTCAGA TGGCAGCAGG AAATCAGAGA CTCCTTTAAG CAATCACGAC 
CCTCGAACTG CTGTGCAACT TCGCACTGCA TTGAAGAGGC TGAAGGAAAT AATGGAGGGA 
AAGAGCCAGG ACAGTGACTT GAAGCAGTAC TGGATGCCGG ACAGCCAGTG TAAAGAATGT 
TATGACTGCA ATGAAAAGTT CACAACCTTC CGACGCCGCC ACCATTGCCG ACTGTGTGGT 
CAGATTTTCT GCAGTCGCTG CTGCAACCAG GAAATCCCTG GAAAGTTCAT GGGTTACACA 
GGAGATTTGC GGGCCTGTAC GTACTGCCGT AAGTTGGCAC TGAGCTACGC CCACTCGGCT 
GATTCGGGCT CCATCGGCGA AGACTTGAAC ACCCTGACTG ACTCTCCGAG CTCTGTGTGT 
GTGCTGGAGC CCAGTGAGCC ACGGACGCCT GTGGGGGGGC GCAAGGCCAG CAGGAACATC 
TTCCTAGAGG AAGACTTAAC ATGGCAGAGA AAAACACCTA TTGGGATGAG AAAGAACATG 
ATTCACCAGG AACCTCAGAG CAGCGGCCTC AGTTCCAGAC TGACAGGACT TCAGGAGGAT 
GTAGGAAAAT CACCTGGTAG GAGGAGGTCT GCTAGCATCA GCACACTTCC ACTTGACCGC 
TCTGGGTCAT CAATGGTACC TTCCTACGAC AGCTCCGTCA GCCCCCCTAC CAGCAGAGCC 
ATGTCGGTGT CAAAGGCTGG GACAAAATTA GATCACAGTG AGGAGGAACG TAAAATACTA 
CTGGACTCCT CCCAGTTGAA GGACTTATGG AGGAAAATCT GTCATAATGG AACAGGGATG 
GAATTCCAGG ACCACAGATA CTGGCTGAGA ACCTACCCCA ACTGCATTGT GGGAAAAGAG 
CTCGTCAACT GGCTGCTAAG AAATGGCACC ATTTCCAACA GGGTCCAAGC TATTGCCATA 
GGTCAGGCAC TCGTGGATGG CCGCTGGCTC GACTGTGTCA CCCACCACGA TCAGCTATTC 
AGAGATGAGT ATGCTCTCTA TCGACCACTT CAGAGTACAG AGTTCTCAGA AACTCCCTCC 
CCGGACAGCG ATAGTGTCAA CTCACTAGAG GGACATTCAG AGCCGTCATG GTTCAAGGAC 
ATCAAATTCG ATGACAGCGA CACCGAGCAG CCCGCAGACG ACACAGAGTA CACCATGCCT 
AGTTCCGCTG GGCCCAGCAA GAGGACATCT GTCAGCAGCG TCCACTCAGC AGTAGACAGT 
GACTCAGCTG CCTCCATCAA CCTCAACATG GAGCAAAACA ATGTGAATTT TCGAATCAAG 
AAAGATTCCA AGTGTCCCCA GGCATTCCCC GCCCTGGAGC ACAAATCTGA AATTATGGCG 
TCTGAAGATG GAGGAGAGAA CATCCTTATA AGTGATGCCT TTATCAAAGA GTCTCTCTTT 
AACCGTCGTG TGGAGGAGAA GGCTAAAGAA ATGCTGTTTA CTCCACTGGG TTGGCACCAC 
AGCTCTCTGG ACCAGCTCAG AGAGGAGAAT GGAGAAAAGA AGGCTATGGA GAGGCTGCTC 
TCGGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAGT TGCTCTACAG CGAGTCTTTG 
TCTCTGTCCT GGCGGGATAT TATCGTTCCT GTGGTTCGCC AGGTTGTCCA GACAGTTCGA 
CCTGATGTTC GCAACAGTGA TGACGACATG GACATCCGCC AACTGGTCCA TATCAAAAAG 
ATTCCTGGAG GAAAAAAGTT TGACTCAACT GTAGTGAATG GCTTTGTGTG TACGAAGAGT 
ATTGCCCACA AAAAGATGAA CCCATATATC AAGAACCCCA AAATTCTGCT GCTGAAATGC 
TCAATAGAGT ATCTGTACAG GGAAGAGACC AAATTCACGT CCATCGACCC CATCGTGCTG 
CAGGAACGAG AGTTTCTGAA AAACTATGTG CAACGTATCG TGGATGTTCG GCCCACTCTG 
GTTCTGGTGG AAAAAACGGT GTCTCGTATA GCTCAGGACA TGCTGCTAGA ACACGGCATT 
ACCCTGGTCA TTAATGTCAA ACCGCAAGTA TTGGACAGGG TGAGTTGTGT GACCCAGGGG 
GACCTTGTCA TGTCCATGGA CCAGCTACTC ACCAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACATGC AGCCCTTCCC TTTGGCCAAT AATGAGGTCA AGACTCTGAT GTTCTTCGAA 
GGATGTCCTG CTCATCTCGG GTGCTCCATA AATCTACGTG GTGCTTCAGA CTACGAGTTA 
GCCAGGGTGA AGGAGATCAT GCGATTAATG GTGTGTGTGG CCTACCACTC TCAGCTGGAG 
ATCTCCTTCC TCATGGATGA GTTTGCCATG CCGCCCAGCT TGTCCCAGAG CACTTCATTT 
CCCTGTCTCC TCGACGGAGG CTCTGCAGAT GAGGAAGCAA GCAGGGAGAA ACTAGAGAAG 
AGTTCTGAAG ATGAAGACAC TGTAGTGGAA GGACAACCTG AGGAGGTGCC CTCTGCCAAT 
GATGTAGACA CTTCCCAGGA CACAAATGAT CCTAAATTAT TCTCCTCTAT GGTGGAAGAG 
ACCACTAATA CTGACACAGT CACGACAAAG TCTTCCACTC CGTTCCAAAG CCCTCGTGCG 
CCATGCTTGC CAGTTCCTCC ACCATTCATC ATGGAAGAAA CCCCAGAAAT GGGCTCAGAC 
AGCCTAACAG GGGCATTTGA GGCAGGGGTG GTGGAATGCT GCCCAAAGGA GGAATCAACC 
AACCTAGAAG ATGGAGAAGA TTCTGATATT CCTACTCCTA AGTTGTTCCG AGACCCCCTG 
CAAGATGATA CTGGGATGTT TGTGGCTGAA GAAGTTGCTT CTTCAGATGA CCATCTCAAG 
TCCATCTCTG CTATCTTCAA ACAGGAACTT AAGGACATCA TCCTGTGCAT TTCACCTTTC 
ATCACATTTG AGAAACCATT TCTTCTTACC CCTGCTGGGA TGCACTGCCC GAGCAGGGAT 
TATTTTCCCG AACAGGTCTA TCTGTCTCCT CTCCTCAACA AGGACTTTAA AGAGTTGGAT 
GGACGCAGAA AGCGGCAGCT CCTTAAGGAG TCCACTCCCT CCCTGGCAAA CAGCCTAACG 
AATGGTGGCC CCCCGTCAAA GCATGTCAGT GTCCTGCCAT CCCACAGTCT CACCAGCACC 
CGGATCGTTG AACTAGTAAA CAGGAGCCAG GGTCTGGCCA GAATGGTTGC AGACTACAGA 
GCAAAGGGTG GACGTATCCA GCACAGCGAA GCTACTGATC CATTTTGCAT AAGCAGCTCT 
TCTGCTCCGG TCAGTGGCCA GAATAGACCA GGTGATGGTT CGGTCAGAGC ACCAGTAAAG 
ACTGACAGCG AAGAGGAGAA GCCAACTAAA CCCAGTGACT GGACCCCCAA GCTGGATTGT 
CTGAACCCCA CCAACCACCA GAGACTCTGT GTGCTTTTTA GCAGTTCATC AACTCAGTCC 
AGCAATGCCC CCAACCCCTG TGTCAACCCA TGGATTGTTA CCATGGAGTT TTATGGCAAG 
AATGACCTCT GCCTTGGTGT ATTCTTGGAG CGATATTGCT TTAGGCCATC ATACCAGTGC 
CCCAGTATAT TTTGTGAGAC CCCCATGGTG CACCACATCC GGCGTTTTGT CCATGGCAAC 
GGCTGCGTGC AGATAGTACT GAAGGAGCTG GACTCCCCTG TGCCGGGGTA CCAGCACACC 
ATTCTCAACT ACTCCTGGTG CCGCATCTGC AAACAAGTGA CACCAGTGGT TCCTCTATCC 
AATGACTCAT GGTCAATGTC TTTTGCAAAA TATCTGGAGC TTCGCTTCTA CGGCCACCAG 
TACACCAGGA GGGCCAATGC AGAGCCCTGT GGCCACTCCA TCCACAAAGA CTACCACCAG 
TACTTCTCAT ACAACCAAAT GGTGGCTTCC TTCAGTTACA CCTCAGTGAG GCTGCTGGAG 
ATTTGTCTTC CTCGTTTCAA AATCTTCATC AGGAATCAGG GTCCTTCGAT CAACAACTTG 
GGGCAGGATC TGAAAGACTT CTCTCAAAAA GTAAATCAGG TTTACGTGGC CATAGATGAT 
CGTCTCACAT CTCTAAAGAC GGACACCTTC AGTAAAACAC GCGAGGAGAA GATGGAGGAC 
CTCTTTGCAC AGAAAGATAT GGAAGAGGCG GAACTGCGCA GCTGGATTGA AAAGTTTCAA 
GCACGATTGC AGGCTTGTGG TCACGTTTCT CCTCAGCAGC TGCAGACGAC TCTGGAATCA 
CTGGTGATGA AGAAACAGAG TCTGTGTGAG ATGTTGCAGT ACTGGAACAG CAGGCTGCAG 
GAATTATTCC AACAGGAGAA AGGCAGGAAA CGTCTGTCAG TGCCCCCAAG CCCCGGCAGG 
CACAGACAGA CTGCCCCTGA TGATGGCAAG AACGCTGTGG AGTCATCCCC ACGTAACCCT 
TCACCTGTGG TACAGAATGG TGAAAAGGAG GACCGTCACC TCACCACACT GCCTTCAACC 
TCCTCTCTGT TGCCCTCGCC TGGAGACCTG GGAGCTGAAC CAGCCACATC TGGTCCTTCC 
CTGAGTGAGC AGGACTCTGT CAGCATCCCA GAAGATGTAT TTGATGGACA CTTGCTGGGC 
TCCAATGACA GTCAGGTGAA GGAGAAATCC ACCATGAAAG CCATCCTTGC CAACTTCCTG 
CCCGGCAACA GTTACAACCC CATTCCATTC CCATTTGACC CAGACAAACA CTACCTGATG 
GATGAGTATG AGCGAGTGCC AATTGCAGTG TGTGAAAGAG AACCAAGCTC CATCATTGCA 
TTTGCTCTGA GTTGTAAGAA GTATAAAACA GCACTGGAAG ATCTGTCCAA GGTGTCAAAC 
ATGGGTGGAG AAGACTCTTC ACAAGCTGCC AGTAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAGTA GGCGTCCAGA AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688450.1
CDS	132..6473
Translation	MAQTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832590.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832590.1

ORF Insert Sequence:

ATGGCTCAGA CCAGCAGTGT CATGGCTGCT GATGATAAGT CATCCTCATC CTCAATGGAG 
TGGACCACTG AGACCCCCGT CACCTTGCCC GTGAGTCCGT CCCACCTGAC TCATTTTAAG 
CCACTGTCAC CCGAGCAGGA TGAGCCCCCG CTCCGTTCAG CATACAGCTC ATTTGTCAAC 
CTATTTCGCT TTAGTAACAA AGTCACAGGC AGTGACCACA GTCTTGTGAA GTCAGGTGAT 
AGAAAGGGCC TGTCTGGATT GATGCCAGGA ACCAAGGAGG GACGTTCTCT TTCAACAGTG 
TCAGAGAAAT CAGACACTCC ATCCCCTTCC CCTCTATCAG AGAGGAGGAG CTGGTCCTCC 
AGCCCACCCC ATTCCCTCTA CACTGCCAGG ATTCCCCGAA AACAACCAGA TCACCTTCAA 
AGGACCTCTG CTGCTTCAGA TGGCAGCAGG AAATCAGAGA CTCCTTTAAG CAATCACGAC 
CCTCGAACTG CTGTGCAACT TCGCACTGCA TTGAAGAGGC TGAAGGAAAT AATGGAGGGA 
AAGAGCCAGG ACAGTGACTT GAAGCAGTAC TGGATGCCGG ACAGCCAGTG TAAAGAATGT 
TATGACTGCA ATGAAAAGTT CACAACCTTC CGACGCCGCC ACCATTGCCG ACTGTGTGGT 
CAGATTTTCT GCAGTCGCTG CTGCAACCAG GAAATCCCTG GAAAGTTCAT GGGTTACACA 
GGAGATTTGC GGGCCTGTAC GTACTGCCGT AAGTTGGCAC TGAGCTACGC CCACTCGGCT 
GATTCGGGCT CCATCGGCGA AGACTTGAAC ACCCTGACTG ACTCTCCGAG CTCTGTGTGT 
GTGCTGGAGC CCAGTGAGCC ACGGACGCCT GTGGGGGGGC GCAAGGCCAG CAGGAACATC 
TTCCTAGAGG AAGACTTAAC ATGGCAGAGA AAAACACCTA TTGGGATGAG AAAGAACATG 
ATTCACCAGG AACCTCAGAG CAGCGGCCTC AGTTCCAGAC TGACAGGACT TCAGGAGGAT 
GTAGGAAAAT CACCTGGTAG GAGGAGGTCT GCTAGCATCA GCACACTTCC ACTTGACCGC 
TCTGGGTCAT CAATGGTACC TTCCTACGAC AGCTCCGTCA GCCCCCCTAC CAGCAGAGCC 
ATGTCGGTGT CAAAGGCTGG GACAAAATTA GATCACAGTG AGGAGGAACG TAAAATACTA 
CTGGACTCCT CCCAGTTGAA GGACTTATGG AGGAAAATCT GTCATAATGG AACAGGGATG 
GAATTCCAGG ACCACAGATA CTGGCTGAGA ACCTACCCCA ACTGCATTGT GGGAAAAGAG 
CTCGTCAACT GGCTGCTAAG AAATGGCACC ATTTCCAACA GGGTCCAAGC TATTGCCATA 
GGTCAGGCAC TCGTGGATGG CCGCTGGCTC GACTGTGTCA CCCACCACGA TCAGCTATTC 
AGAGATGAGT ATGCTCTCTA TCGACCACTT CAGAGTACAG AGTTCTCAGA AACTCCCTCC 
CCGGACAGCG ATAGTGTCAA CTCACTAGAG GGACATTCAG AGCCGTCATG GTTCAAGGAC 
ATCAAATTCG ATGACAGCGA CACCGAGCAG CCCGCAGACG ACACAGAGTA CACCATGCCT 
AGTTCCGCTG GGCCCAGCAA GAGGACATCT GTCAGCAGCG TCCACTCAGC AGTAGACAGT 
GACTCAGCTG CCTCCATCAA CCTCAACATG GAGCAAAACA ATGTGAATTT TCGAATCAAG 
AAAGATTCCA AGTGTCCCCA GGCATTCCCC GCCCTGGAGC ACAAATCTGA AATTATGGCG 
TCTGAAGATG GAGGAGAGAA CATCCTTATA AGTGATGCCT TTATCAAAGA GTCTCTCTTT 
AACCGTCGTG TGGAGGAGAA GGCTAAAGAA ATGCTGTTTA CTCCACTGGG TTGGCACCAC 
AGCTCTCTGG ACCAGCTCAG AGAGGAGAAT GGAGAAAAGA AGGCTATGGA GAGGCTGCTC 
TCGGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAGT TGCTCTACAG CGAGTCTTTG 
TCTCTGTCCT GGCGGGATAT TATCGTTCCT GTGGTTCGCC AGGTTGTCCA GACAGTTCGA 
CCTGATGTTC GCAACAGTGA TGACGACATG GACATCCGCC AACTGGTCCA TATCAAAAAG 
ATTCCTGGAG GAAAAAAGTT TGACTCAACT GTAGTGAATG GCTTTGTGTG TACGAAGAGT 
ATTGCCCACA AAAAGATGAA CCCATATATC AAGAACCCCA AAATTCTGCT GCTGAAATGC 
TCAATAGAGT ATCTGTACAG GGAAGAGACC AAATTCACGT CCATCGACCC CATCGTGCTG 
CAGGAACGAG AGTTTCTGAA AAACTATGTG CAACGTATCG TGGATGTTCG GCCCACTCTG 
GTTCTGGTGG AAAAAACGGT GTCTCGTATA GCTCAGGACA TGCTGCTAGA ACACGGCATT 
ACCCTGGTCA TTAATGTCAA ACCGCAAGTA TTGGACAGGG TGAGTTGTGT GACCCAGGGG 
GACCTTGTCA TGTCCATGGA CCAGCTACTC ACCAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACATGC AGCCCTTCCC TTTGGCCAAT AATGAGGTCA AGACTCTGAT GTTCTTCGAA 
GGATGTCCTG CTCATCTCGG GTGCTCCATA AATCTACGTG GTGCTTCAGA CTACGAGTTA 
GCCAGGGTGA AGGAGATCAT GCGATTAATG GTGTGTGTGG CCTACCACTC TCAGCTGGAG 
ATCTCCTTCC TCATGGATGA GTTTGCCATG CCGCCCAGCT TGTCCCAGAG CACTTCATTT 
CCCTGTCTCC TCGACGGAGG CTCTGCAGAT GAGGAAGCAA GCAGGGAGAA ACTAGAGAAG 
AGTTCTGAAG ATGAAGACAC TGTAGTGGAA GGACAACCTG AGGAGGTGCC CTCTGCCAAT 
GATGTAGACA CTTCCCAGGA CACAAATGAT CCTAAATTAT TCTCCTCTAT GGTGGAAGAG 
ACCACTAATA CTGACACAGT CACGACAAAG TCTTCCACTC CGTTCCAAAG CCCTCGTGCG 
CCATGCTTGC CAGTTCCTCC ACCATTCATC ATGGAAGAAA CCCCAGAAAT GGGCTCAGAC 
AGCCTAACAG GGGCATTTGA GGCAGGGGTG GTGGAATGCT GCCCAAAGGA GGAATCAACC 
AACCTAGAAG ATGGAGAAGA TTCTGATATT CCTACTCCTA AGTTGTTCCG AGACCCCCTG 
CAAGATGATA CTGGGATGTT TGTGGCTGAA GAAGTTGCTT CTTCAGATGA CCATCTCAAG 
TCCATCTCTG CTATCTTCAA ACAGGAACTT AAGGACATCA TCCTGTGCAT TTCACCTTTC 
ATCACATTTG AGAAACCATT TCTTCTTACC CCTGCTGGGA TGCACTGCCC GAGCAGGGAT 
TATTTTCCCG AACAGGTCTA TCTGTCTCCT CTCCTCAACA AGGACTTTAA AGAGTTGGAT 
GGACGCAGAA AGCGGCAGCT CCTTAAGGAG TCCACTCCCT CCCTGGCAAA CAGCCTAACG 
AATGGTGGCC CCCCGTCAAA GCATGTCAGT GTCCTGCCAT CCCACAGTCT CACCAGCACC 
CGGATCGTTG AACTAGTAAA CAGGAGCCAG GGTCTGGCCA GAATGGTTGC AGACTACAGA 
GCAAAGGGTG GACGTATCCA GCACAGCGAA GCTACTGATC CATTTTGCAT AAGCAGCTCT 
TCTGCTCCGG TCAGTGGCCA GAATAGACCA GGTGATGGTT CGGTCAGAGC ACCAGTAAAG 
ACTGACAGCG AAGAGGAGAA GCCAACTAAA CCCAGTGACT GGACCCCCAA GCTGGATTGT 
CTGAACCCCA CCAACCACCA GAGACTCTGT GTGCTTTTTA GCAGTTCATC AACTCAGTCC 
AGCAATGCCC CCAACCCCTG TGTCAACCCA TGGATTGTTA CCATGGAGTT TTATGGCAAG 
AATGACCTCT GCCTTGGTGT ATTCTTGGAG CGATATTGCT TTAGGCCATC ATACCAGTGC 
CCCAGTATAT TTTGTGAGAC CCCCATGGTG CACCACATCC GGCGTTTTGT CCATGGCAAC 
GGCTGCGTGC AGATAGTACT GAAGGAGCTG GACTCCCCTG TGCCGGGGTA CCAGCACACC 
ATTCTCAACT ACTCCTGGTG CCGCATCTGC AAACAAGTGA CACCAGTGGT TCCTCTATCC 
AATGACTCAT GGTCAATGTC TTTTGCAAAA TATCTGGAGC TTCGCTTCTA CGGCCACCAG 
TACACCAGGA GGGCCAATGC AGAGCCCTGT GGCCACTCCA TCCACAAAGA CTACCACCAG 
TACTTCTCAT ACAACCAAAT GGTGGCTTCC TTCAGTTACA CCTCAGTGAG GCTGCTGGAG 
ATTTGTCTTC CTCGTTTCAA AATCTTCATC AGGAATCAGG GTCCTTCGAT CAACAACTTG 
GGGCAGGATC TGAAAGACTT CTCTCAAAAA GTAAATCAGG TTTACGTGGC CATAGATGAT 
CGTCTCACAT CTCTAAAGAC GGACACCTTC AGTAAAACAC GCGAGGAGAA GATGGAGGAC 
CTCTTTGCAC AGAAAGATAT GGAAGAGGCG GAACTGCGCA GCTGGATTGA AAAGTTTCAA 
GCACGATTGC AGGCTTGTGG TCACGTTTCT CCTCAGCAGC TGCAGACGAC TCTGGAATCA 
CTGGTGATGA AGAAACAGAG TCTGTGTGAG ATGTTGCAGT ACTGGAACAG CAGGCTGCAG 
GAATTATTCC AACAGGAGAA AGGCAGGAAA CGTCTGTCAG TGCCCCCAAG CCCCGGCAGG 
CACAGACAGA CTGCCCCTGA TGATGGCAAG AACGCTGTGG AGTCATCCCC ACGTAACCCT 
TCACCTGTGG TACAGAATGG TGAAAAGGAG GACCGTCACC TCACCACACT GCCTTCAACC 
TCCTCTCTGT TGCCCTCGCC TGGAGACCTG GGAGCTGAAC CAGCCACATC TGGTCCTTCC 
CTGAGTGAGC AGGACTCTGT CAGCATCCCA GAAGATGTAT TTGATGGACA CTTGCTGGGC 
TCCAATGACA GTCAGGTGAA GGAGAAATCC ACCATGAAAG CCATCCTTGC CAACTTCCTG 
CCCGGCAACA GTTACAACCC CATTCCATTC CCATTTGACC CAGACAAACA CTACCTGATG 
GATGAGTATG AGCGAGTGCC AATTGCAGTG TGTGAAAGAG AACCAAGCTC CATCATTGCA 
TTTGCTCTGA GTTGTAAGAA GTATAAAACA GCACTGGAAG ATCTGTCCAA GGTGTCAAAC 
ATGGGTGGAG AAGACTCTTC ACAAGCTGCC AGTAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAGTA GGCGTCCAGA AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832594.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688454.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X3, mRNA.

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CloneID	OTo40922
Clone ID Related Accession (Same CDS sequence)	XM_029832594.1
Accession Version	XM_029832594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA GTAGGCGTCC AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688454.1
CDS	132..6473
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832594.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832594.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA GTAGGCGTCC AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832598.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688458.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X4, mRNA.

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CloneID	OTo40923
Clone ID Related Accession (Same CDS sequence)	XM_029832598.1
Accession Version	XM_029832598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAGTA GGCGTCCAGA AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688458.1
CDS	134..6475
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832598.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832598.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAGTA GGCGTCCAGA AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832601.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688461.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X5, mRNA.

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CloneID	OTo40924
Clone ID Related Accession (Same CDS sequence)	XM_029832601.1
Accession Version	XM_029832601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGGCAGT GACCACAGTC TTGTGAAGTC AGGTGATAGA 
AAGGGCCTGT CTGGATTGAT GCCAGGAACC AAGGAGGGAC GTTCTCTTTC AACAGTGTCA 
GAGAAATCAG ACACTCCATC CCCTTCCCCT CTATCAGAGA GGAGGAGCTG GTCCTCCAGC 
CCACCCCATT CCCTCTACAC TGCCAGGATT CCCCGAAAAC AACCAGATCA CCTTCAAAGG 
ACCTCTGCTG CTTCAGATGG CAGCAGGAAA TCAGAGACTC CTTTAAGCAA TCACGACCCT 
CGAACTGCTG TGCAACTTCG CACTGCATTG AAGAGGCTGA AGGAAATAAT GGAGGGAAAG 
AGCCAGGACA GTGACTTGAA GCAGTACTGG ATGCCGGACA GCCAGTGTAA AGAATGTTAT 
GACTGCAATG AAAAGTTCAC AACCTTCCGA CGCCGCCACC ATTGCCGACT GTGTGGTCAG 
ATTTTCTGCA GTCGCTGCTG CAACCAGGAA ATCCCTGGAA AGTTCATGGG TTACACAGGA 
GATTTGCGGG CCTGTACGTA CTGCCGTAAG TTGGCACTGA GCTACGCCCA CTCGGCTGAT 
TCGGGCTCCA TCGGCGAAGA CTTGAACACC CTGACTGACT CTCCGAGCTC TGTGTGTGTG 
CTGGAGCCCA GTGAGCCACG GACGCCTGTG GGGGGGCGCA AGGCCAGCAG GAACATCTTC 
CTAGAGGAAG ACTTAACATG GCAGAGAAAA ACACCTATTG GGATGAGAAA GAACATGATT 
CACCAGGAAC CTCAGAGCAG CGGCCTCAGT TCCAGACTGA CAGGACTTCA GGAGGATGTA 
GGAAAATCAC CTGGTAGGAG GAGGTCTGCT AGCATCAGCA CACTTCCACT TGACCGCTCT 
GGGTCATCAA TGGTACCTTC CTACGACAGC TCCGTCAGCC CCCCTACCAG CAGAGCCATG 
TCGGTGTCAA AGGCTGGGAC AAAATTAGAT CACAGTGAGG AGGAACGTAA AATACTACTG 
GACTCCTCCC AGTTGAAGGA CTTATGGAGG AAAATCTGTC ATAATGGAAC AGGGATGGAA 
TTCCAGGACC ACAGATACTG GCTGAGAACC TACCCCAACT GCATTGTGGG AAAAGAGCTC 
GTCAACTGGC TGCTAAGAAA TGGCACCATT TCCAACAGGG TCCAAGCTAT TGCCATAGGT 
CAGGCACTCG TGGATGGCCG CTGGCTCGAC TGTGTCACCC ACCACGATCA GCTATTCAGA 
GATGAGTATG CTCTCTATCG ACCACTTCAG AGTACAGAGT TCTCAGAAAC TCCCTCCCCG 
GACAGCGATA GTGTCAACTC ACTAGAGGGA CATTCAGAGC CGTCATGGTT CAAGGACATC 
AAATTCGATG ACAGCGACAC CGAGCAGCCC GCAGACGACA CAGAGTACAC CATGCCTAGT 
TCCGCTGGGC CCAGCAAGAG GACATCTGTC AGCAGCGTCC ACTCAGCAGT AGACAGTGAC 
TCAGCTGCCT CCATCAACCT CAACATGGAG CAAAACAATG TGAATTTTCG AATCAAGAAA 
GATTCCAAGT GTCCCCAGGC ATTCCCCGCC CTGGAGCACA AATCTGAAAT TATGGCGTCT 
GAAGATGGAG GAGAGAACAT CCTTATAAGT GATGCCTTTA TCAAAGAGTC TCTCTTTAAC 
CGTCGTGTGG AGGAGAAGGC TAAAGAAATG CTGTTTACTC CACTGGGTTG GCACCACAGC 
TCTCTGGACC AGCTCAGAGA GGAGAATGGA GAAAAGAAGG CTATGGAGAG GCTGCTCTCG 
GCAAATCACA GCCACATGAT GGCGCTGCTG CAGCAGTTGC TCTACAGCGA GTCTTTGTCT 
CTGTCCTGGC GGGATATTAT CGTTCCTGTG GTTCGCCAGG TTGTCCAGAC AGTTCGACCT 
GATGTTCGCA ACAGTGATGA CGACATGGAC ATCCGCCAAC TGGTCCATAT CAAAAAGATT 
CCTGGAGGAA AAAAGTTTGA CTCAACTGTA GTGAATGGCT TTGTGTGTAC GAAGAGTATT 
GCCCACAAAA AGATGAACCC ATATATCAAG AACCCCAAAA TTCTGCTGCT GAAATGCTCA 
ATAGAGTATC TGTACAGGGA AGAGACCAAA TTCACGTCCA TCGACCCCAT CGTGCTGCAG 
GAACGAGAGT TTCTGAAAAA CTATGTGCAA CGTATCGTGG ATGTTCGGCC CACTCTGGTT 
CTGGTGGAAA AAACGGTGTC TCGTATAGCT CAGGACATGC TGCTAGAACA CGGCATTACC 
CTGGTCATTA ATGTCAAACC GCAAGTATTG GACAGGGTGA GTTGTGTGAC CCAGGGGGAC 
CTTGTCATGT CCATGGACCA GCTACTCACC AAACCTCGTC TGGGTACCTG CCACAAGTTT 
TACATGCAGC CCTTCCCTTT GGCCAATAAT GAGGTCAAGA CTCTGATGTT CTTCGAAGGA 
TGTCCTGCTC ATCTCGGGTG CTCCATAAAT CTACGTGGTG CTTCAGACTA CGAGTTAGCC 
AGGGTGAAGG AGATCATGCG ATTAATGGTG TGTGTGGCCT ACCACTCTCA GCTGGAGATC 
TCCTTCCTCA TGGATGAGTT TGCCATGCCG CCCAGCTTGT CCCAGAGCAC TTCATTTCCC 
TGTCTCCTCG ACGGAGGCTC TGCAGATGAG GAAGCAAGCA GGGAGAAACT AGAGAAGAGT 
TCTGAAGATG AAGACACTGT AGTGGAAGGA CAACCTGAGG AGGTGCCCTC TGCCAATGAT 
GTAGACACTT CCCAGGACAC AAATGATCCT AAATTATTCT CCTCTATGGT GGAAGAGACC 
ACTAATACTG ACACAGTCAC GACAAAGTCT TCCACTCCGT TCCAAAGCCC TCGTGCGCCA 
TGCTTGCCAG TTCCTCCACC ATTCATCATG GAAGAAACCC CAGAAATGGG CTCAGACAGC 
CTAACAGGGG CATTTGAGGC AGGGGTGGTG GAATGCTGCC CAAAGGAGGA ATCAACCAAC 
CTAGAAGATG GAGAAGATTC TGATATTCCT ACTCCTAAGT TGTTCCGAGA CCCCCTGCAA 
GATGATACTG GGATGTTTGT GGCTGAAGAA GTTGCTTCTT CAGATGACCA TCTCAAGTCC 
ATCTCTGCTA TCTTCAAACA GGAACTTAAG GACATCATCC TGTGCATTTC ACCTTTCATC 
ACATTTGAGA AACCATTTCT TCTTACCCCT GCTGGGATGC ACTGCCCGAG CAGGGATTAT 
TTTCCCGAAC AGGTCTATCT GTCTCCTCTC CTCAACAAGG ACTTTAAAGA GTTGGATGGA 
CGCAGAAAGC GGCAGCTCCT TAAGGAGTCC ACTCCCTCCC TGGCAAACAG CCTAACGAAT 
GGTGGCCCCC CGTCAAAGCA TGTCAGTGTC CTGCCATCCC ACAGTCTCAC CAGCACCCGG 
ATCGTTGAAC TAGTAAACAG GAGCCAGGGT CTGGCCAGAA TGGTTGCAGA CTACAGAGCA 
AAGGGTGGAC GTATCCAGCA CAGCGAAGCT ACTGATCCAT TTTGCATAAG CAGCTCTTCT 
GCTCCGGTCA GTGGCCAGAA TAGACCAGGT GATGGTTCGG TCAGAGCACC AGTAAAGACT 
GACAGCGAAG AGGAGAAGCC AACTAAACCC AGTGACTGGA CCCCCAAGCT GGATTGTCTG 
AACCCCACCA ACCACCAGAG ACTCTGTGTG CTTTTTAGCA GTTCATCAAC TCAGTCCAGC 
AATGCCCCCA ACCCCTGTGT CAACCCATGG ATTGTTACCA TGGAGTTTTA TGGCAAGAAT 
GACCTCTGCC TTGGTGTATT CTTGGAGCGA TATTGCTTTA GGCCATCATA CCAGTGCCCC 
AGTATATTTT GTGAGACCCC CATGGTGCAC CACATCCGGC GTTTTGTCCA TGGCAACGGC 
TGCGTGCAGA TAGTACTGAA GGAGCTGGAC TCCCCTGTGC CGGGGTACCA GCACACCATT 
CTCAACTACT CCTGGTGCCG CATCTGCAAA CAAGTGACAC CAGTGGTTCC TCTATCCAAT 
GACTCATGGT CAATGTCTTT TGCAAAATAT CTGGAGCTTC GCTTCTACGG CCACCAGTAC 
ACCAGGAGGG CCAATGCAGA GCCCTGTGGC CACTCCATCC ACAAAGACTA CCACCAGTAC 
TTCTCATACA ACCAAATGGT GGCTTCCTTC AGTTACACCT CAGTGAGGCT GCTGGAGATT 
TGTCTTCCTC GTTTCAAAAT CTTCATCAGG AATCAGGGTC CTTCGATCAA CAACTTGGGG 
CAGGATCTGA AAGACTTCTC TCAAAAAGTA AATCAGGTTT ACGTGGCCAT AGATGATCGT 
CTCACATCTC TAAAGACGGA CACCTTCAGT AAAACACGCG AGGAGAAGAT GGAGGACCTC 
TTTGCACAGA AAGATATGGA AGAGGCGGAA CTGCGCAGCT GGATTGAAAA GTTTCAAGCA 
CGATTGCAGG CTTGTGGTCA CGTTTCTCCT CAGCAGCTGC AGACGACTCT GGAATCACTG 
GTGATGAAGA AACAGAGTCT GTGTGAGATG TTGCAGTACT GGAACAGCAG GCTGCAGGAA 
TTATTCCAAC AGGAGAAAGG CAGGAAACGT CTGTCAGTGC CCCCAAGCCC CGGCAGGCAC 
AGACAGACTG CCCCTGATGA TGGCAAGAAC GCTGTGGAGT CATCCCCACG TAACCCTTCA 
CCTGTGGTAC AGAATGGTGA AAAGGAGGAC CGTCACCTCA CCACACTGCC TTCAACCTCC 
TCTCTGTTGC CCTCGCCTGG AGACCTGGGA GCTGAACCAG CCACATCTGG TCCTTCCCTG 
AGTGAGCAGG ACTCTGTCAG CATCCCAGAA GATGTATTTG ATGGACACTT GCTGGGCTCC 
AATGACAGTC AGGTGAAGGA GAAATCCACC ATGAAAGCCA TCCTTGCCAA CTTCCTGCCC 
GGCAACAGTT ACAACCCCAT TCCATTCCCA TTTGACCCAG ACAAACACTA CCTGATGGAT 
GAGTATGAGC GAGTGCCAAT TGCAGTGTGT GAAAGAGAAC CAAGCTCCAT CATTGCATTT 
GCTCTGAGTT GTAAGAAGTA TAAAACAGCA CTGGAAGATC TGTCCAAGGT GTCAAACATG 
GGTGGAGAAG ACTCTTCACA AGCTGCCAGT AGTGGAGAGA GTCGGGTGAG GGGCAGCCCC 
GCCAGGCCCA GTGAGTCAGC CTCGTCCCAA CAGAGCCGCA GCAGCAGTGA AGCAGATCCC 
CTCAGTAGGC GTCCAGAAGA CGCAGACCTA ACAGACAAAC AGAAGAAGCA GGCCCTGAAT 
CCCCACATTG AGCTACAGTT CTCTGATGCA AGCGCAAAGT TTTACTGTCG GATCTACTAC 
GCCGAGGAGT TTCATAAGAT GCGTGAGGAG ATCATGGAGA GCTCCGAAGA GGACTTTGTG 
CGTTCCCTGT CGCACTGTGT CAACTGGCAG GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC 
TACGCAACAG AAGACGACCG TTTCATCCTA AAACAGATGC CCAGGCTGGA GGTGCAGTCC 
TTCTTGGACT TTGCTCCGCA CTACTTCACC TACATCACCG GAGCTGTGCA GCAGAAGAGG 
CCAACTGCAT TGGCAAAGAT TCTCGGAGTT TACAGGATAG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAACTGGA CCTGCTCGTC ATGGAAAACC TGTTCTACGG ACGCAAGATG 
GCTCAGGTCT TTGACCTGAA AGGTTCCCTC AGAAACCGTC ATGTGAAGAC AGACCTGGTA 
AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT GAGAATCTAC TGAAGCTGAT CCACGACAAT 
CCGCTCTACA TTCGCTCTCA CTGCAAGTCC ATCTTGCGCG CCGCCATACA CAGCGACGCC 
TACTTCCTGT CCAGCCACCT CATCATTGAC TACTCCCTGC TGGTGGGGTG GGACGATGCC 
ACCGACCAGC TGGTGGTTGG GATCATAGAC TATATTCGGA CGTTTACCTG GGACAAGAAA 
TTAGAGATGG TTGTCAAATC CACTGGAATC CTGGGGGGTC AGGGAAAGAT GCCCACCGTT 
GTTTCTCCTG AGCTGTACCG AGCTCGGTTC TGTGAGGCCA TGGACAAATA CTTCCTGATG 
GTACCCGACC ACTGGACGGG TCTTGGCATC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688461.1
CDS	132..6470
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832601.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832601.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGGCAGT GACCACAGTC TTGTGAAGTC AGGTGATAGA 
AAGGGCCTGT CTGGATTGAT GCCAGGAACC AAGGAGGGAC GTTCTCTTTC AACAGTGTCA 
GAGAAATCAG ACACTCCATC CCCTTCCCCT CTATCAGAGA GGAGGAGCTG GTCCTCCAGC 
CCACCCCATT CCCTCTACAC TGCCAGGATT CCCCGAAAAC AACCAGATCA CCTTCAAAGG 
ACCTCTGCTG CTTCAGATGG CAGCAGGAAA TCAGAGACTC CTTTAAGCAA TCACGACCCT 
CGAACTGCTG TGCAACTTCG CACTGCATTG AAGAGGCTGA AGGAAATAAT GGAGGGAAAG 
AGCCAGGACA GTGACTTGAA GCAGTACTGG ATGCCGGACA GCCAGTGTAA AGAATGTTAT 
GACTGCAATG AAAAGTTCAC AACCTTCCGA CGCCGCCACC ATTGCCGACT GTGTGGTCAG 
ATTTTCTGCA GTCGCTGCTG CAACCAGGAA ATCCCTGGAA AGTTCATGGG TTACACAGGA 
GATTTGCGGG CCTGTACGTA CTGCCGTAAG TTGGCACTGA GCTACGCCCA CTCGGCTGAT 
TCGGGCTCCA TCGGCGAAGA CTTGAACACC CTGACTGACT CTCCGAGCTC TGTGTGTGTG 
CTGGAGCCCA GTGAGCCACG GACGCCTGTG GGGGGGCGCA AGGCCAGCAG GAACATCTTC 
CTAGAGGAAG ACTTAACATG GCAGAGAAAA ACACCTATTG GGATGAGAAA GAACATGATT 
CACCAGGAAC CTCAGAGCAG CGGCCTCAGT TCCAGACTGA CAGGACTTCA GGAGGATGTA 
GGAAAATCAC CTGGTAGGAG GAGGTCTGCT AGCATCAGCA CACTTCCACT TGACCGCTCT 
GGGTCATCAA TGGTACCTTC CTACGACAGC TCCGTCAGCC CCCCTACCAG CAGAGCCATG 
TCGGTGTCAA AGGCTGGGAC AAAATTAGAT CACAGTGAGG AGGAACGTAA AATACTACTG 
GACTCCTCCC AGTTGAAGGA CTTATGGAGG AAAATCTGTC ATAATGGAAC AGGGATGGAA 
TTCCAGGACC ACAGATACTG GCTGAGAACC TACCCCAACT GCATTGTGGG AAAAGAGCTC 
GTCAACTGGC TGCTAAGAAA TGGCACCATT TCCAACAGGG TCCAAGCTAT TGCCATAGGT 
CAGGCACTCG TGGATGGCCG CTGGCTCGAC TGTGTCACCC ACCACGATCA GCTATTCAGA 
GATGAGTATG CTCTCTATCG ACCACTTCAG AGTACAGAGT TCTCAGAAAC TCCCTCCCCG 
GACAGCGATA GTGTCAACTC ACTAGAGGGA CATTCAGAGC CGTCATGGTT CAAGGACATC 
AAATTCGATG ACAGCGACAC CGAGCAGCCC GCAGACGACA CAGAGTACAC CATGCCTAGT 
TCCGCTGGGC CCAGCAAGAG GACATCTGTC AGCAGCGTCC ACTCAGCAGT AGACAGTGAC 
TCAGCTGCCT CCATCAACCT CAACATGGAG CAAAACAATG TGAATTTTCG AATCAAGAAA 
GATTCCAAGT GTCCCCAGGC ATTCCCCGCC CTGGAGCACA AATCTGAAAT TATGGCGTCT 
GAAGATGGAG GAGAGAACAT CCTTATAAGT GATGCCTTTA TCAAAGAGTC TCTCTTTAAC 
CGTCGTGTGG AGGAGAAGGC TAAAGAAATG CTGTTTACTC CACTGGGTTG GCACCACAGC 
TCTCTGGACC AGCTCAGAGA GGAGAATGGA GAAAAGAAGG CTATGGAGAG GCTGCTCTCG 
GCAAATCACA GCCACATGAT GGCGCTGCTG CAGCAGTTGC TCTACAGCGA GTCTTTGTCT 
CTGTCCTGGC GGGATATTAT CGTTCCTGTG GTTCGCCAGG TTGTCCAGAC AGTTCGACCT 
GATGTTCGCA ACAGTGATGA CGACATGGAC ATCCGCCAAC TGGTCCATAT CAAAAAGATT 
CCTGGAGGAA AAAAGTTTGA CTCAACTGTA GTGAATGGCT TTGTGTGTAC GAAGAGTATT 
GCCCACAAAA AGATGAACCC ATATATCAAG AACCCCAAAA TTCTGCTGCT GAAATGCTCA 
ATAGAGTATC TGTACAGGGA AGAGACCAAA TTCACGTCCA TCGACCCCAT CGTGCTGCAG 
GAACGAGAGT TTCTGAAAAA CTATGTGCAA CGTATCGTGG ATGTTCGGCC CACTCTGGTT 
CTGGTGGAAA AAACGGTGTC TCGTATAGCT CAGGACATGC TGCTAGAACA CGGCATTACC 
CTGGTCATTA ATGTCAAACC GCAAGTATTG GACAGGGTGA GTTGTGTGAC CCAGGGGGAC 
CTTGTCATGT CCATGGACCA GCTACTCACC AAACCTCGTC TGGGTACCTG CCACAAGTTT 
TACATGCAGC CCTTCCCTTT GGCCAATAAT GAGGTCAAGA CTCTGATGTT CTTCGAAGGA 
TGTCCTGCTC ATCTCGGGTG CTCCATAAAT CTACGTGGTG CTTCAGACTA CGAGTTAGCC 
AGGGTGAAGG AGATCATGCG ATTAATGGTG TGTGTGGCCT ACCACTCTCA GCTGGAGATC 
TCCTTCCTCA TGGATGAGTT TGCCATGCCG CCCAGCTTGT CCCAGAGCAC TTCATTTCCC 
TGTCTCCTCG ACGGAGGCTC TGCAGATGAG GAAGCAAGCA GGGAGAAACT AGAGAAGAGT 
TCTGAAGATG AAGACACTGT AGTGGAAGGA CAACCTGAGG AGGTGCCCTC TGCCAATGAT 
GTAGACACTT CCCAGGACAC AAATGATCCT AAATTATTCT CCTCTATGGT GGAAGAGACC 
ACTAATACTG ACACAGTCAC GACAAAGTCT TCCACTCCGT TCCAAAGCCC TCGTGCGCCA 
TGCTTGCCAG TTCCTCCACC ATTCATCATG GAAGAAACCC CAGAAATGGG CTCAGACAGC 
CTAACAGGGG CATTTGAGGC AGGGGTGGTG GAATGCTGCC CAAAGGAGGA ATCAACCAAC 
CTAGAAGATG GAGAAGATTC TGATATTCCT ACTCCTAAGT TGTTCCGAGA CCCCCTGCAA 
GATGATACTG GGATGTTTGT GGCTGAAGAA GTTGCTTCTT CAGATGACCA TCTCAAGTCC 
ATCTCTGCTA TCTTCAAACA GGAACTTAAG GACATCATCC TGTGCATTTC ACCTTTCATC 
ACATTTGAGA AACCATTTCT TCTTACCCCT GCTGGGATGC ACTGCCCGAG CAGGGATTAT 
TTTCCCGAAC AGGTCTATCT GTCTCCTCTC CTCAACAAGG ACTTTAAAGA GTTGGATGGA 
CGCAGAAAGC GGCAGCTCCT TAAGGAGTCC ACTCCCTCCC TGGCAAACAG CCTAACGAAT 
GGTGGCCCCC CGTCAAAGCA TGTCAGTGTC CTGCCATCCC ACAGTCTCAC CAGCACCCGG 
ATCGTTGAAC TAGTAAACAG GAGCCAGGGT CTGGCCAGAA TGGTTGCAGA CTACAGAGCA 
AAGGGTGGAC GTATCCAGCA CAGCGAAGCT ACTGATCCAT TTTGCATAAG CAGCTCTTCT 
GCTCCGGTCA GTGGCCAGAA TAGACCAGGT GATGGTTCGG TCAGAGCACC AGTAAAGACT 
GACAGCGAAG AGGAGAAGCC AACTAAACCC AGTGACTGGA CCCCCAAGCT GGATTGTCTG 
AACCCCACCA ACCACCAGAG ACTCTGTGTG CTTTTTAGCA GTTCATCAAC TCAGTCCAGC 
AATGCCCCCA ACCCCTGTGT CAACCCATGG ATTGTTACCA TGGAGTTTTA TGGCAAGAAT 
GACCTCTGCC TTGGTGTATT CTTGGAGCGA TATTGCTTTA GGCCATCATA CCAGTGCCCC 
AGTATATTTT GTGAGACCCC CATGGTGCAC CACATCCGGC GTTTTGTCCA TGGCAACGGC 
TGCGTGCAGA TAGTACTGAA GGAGCTGGAC TCCCCTGTGC CGGGGTACCA GCACACCATT 
CTCAACTACT CCTGGTGCCG CATCTGCAAA CAAGTGACAC CAGTGGTTCC TCTATCCAAT 
GACTCATGGT CAATGTCTTT TGCAAAATAT CTGGAGCTTC GCTTCTACGG CCACCAGTAC 
ACCAGGAGGG CCAATGCAGA GCCCTGTGGC CACTCCATCC ACAAAGACTA CCACCAGTAC 
TTCTCATACA ACCAAATGGT GGCTTCCTTC AGTTACACCT CAGTGAGGCT GCTGGAGATT 
TGTCTTCCTC GTTTCAAAAT CTTCATCAGG AATCAGGGTC CTTCGATCAA CAACTTGGGG 
CAGGATCTGA AAGACTTCTC TCAAAAAGTA AATCAGGTTT ACGTGGCCAT AGATGATCGT 
CTCACATCTC TAAAGACGGA CACCTTCAGT AAAACACGCG AGGAGAAGAT GGAGGACCTC 
TTTGCACAGA AAGATATGGA AGAGGCGGAA CTGCGCAGCT GGATTGAAAA GTTTCAAGCA 
CGATTGCAGG CTTGTGGTCA CGTTTCTCCT CAGCAGCTGC AGACGACTCT GGAATCACTG 
GTGATGAAGA AACAGAGTCT GTGTGAGATG TTGCAGTACT GGAACAGCAG GCTGCAGGAA 
TTATTCCAAC AGGAGAAAGG CAGGAAACGT CTGTCAGTGC CCCCAAGCCC CGGCAGGCAC 
AGACAGACTG CCCCTGATGA TGGCAAGAAC GCTGTGGAGT CATCCCCACG TAACCCTTCA 
CCTGTGGTAC AGAATGGTGA AAAGGAGGAC CGTCACCTCA CCACACTGCC TTCAACCTCC 
TCTCTGTTGC CCTCGCCTGG AGACCTGGGA GCTGAACCAG CCACATCTGG TCCTTCCCTG 
AGTGAGCAGG ACTCTGTCAG CATCCCAGAA GATGTATTTG ATGGACACTT GCTGGGCTCC 
AATGACAGTC AGGTGAAGGA GAAATCCACC ATGAAAGCCA TCCTTGCCAA CTTCCTGCCC 
GGCAACAGTT ACAACCCCAT TCCATTCCCA TTTGACCCAG ACAAACACTA CCTGATGGAT 
GAGTATGAGC GAGTGCCAAT TGCAGTGTGT GAAAGAGAAC CAAGCTCCAT CATTGCATTT 
GCTCTGAGTT GTAAGAAGTA TAAAACAGCA CTGGAAGATC TGTCCAAGGT GTCAAACATG 
GGTGGAGAAG ACTCTTCACA AGCTGCCAGT AGTGGAGAGA GTCGGGTGAG GGGCAGCCCC 
GCCAGGCCCA GTGAGTCAGC CTCGTCCCAA CAGAGCCGCA GCAGCAGTGA AGCAGATCCC 
CTCAGTAGGC GTCCAGAAGA CGCAGACCTA ACAGACAAAC AGAAGAAGCA GGCCCTGAAT 
CCCCACATTG AGCTACAGTT CTCTGATGCA AGCGCAAAGT TTTACTGTCG GATCTACTAC 
GCCGAGGAGT TTCATAAGAT GCGTGAGGAG ATCATGGAGA GCTCCGAAGA GGACTTTGTG 
CGTTCCCTGT CGCACTGTGT CAACTGGCAG GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC 
TACGCAACAG AAGACGACCG TTTCATCCTA AAACAGATGC CCAGGCTGGA GGTGCAGTCC 
TTCTTGGACT TTGCTCCGCA CTACTTCACC TACATCACCG GAGCTGTGCA GCAGAAGAGG 
CCAACTGCAT TGGCAAAGAT TCTCGGAGTT TACAGGATAG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAACTGGA CCTGCTCGTC ATGGAAAACC TGTTCTACGG ACGCAAGATG 
GCTCAGGTCT TTGACCTGAA AGGTTCCCTC AGAAACCGTC ATGTGAAGAC AGACCTGGTA 
AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT GAGAATCTAC TGAAGCTGAT CCACGACAAT 
CCGCTCTACA TTCGCTCTCA CTGCAAGTCC ATCTTGCGCG CCGCCATACA CAGCGACGCC 
TACTTCCTGT CCAGCCACCT CATCATTGAC TACTCCCTGC TGGTGGGGTG GGACGATGCC 
ACCGACCAGC TGGTGGTTGG GATCATAGAC TATATTCGGA CGTTTACCTG GGACAAGAAA 
TTAGAGATGG TTGTCAAATC CACTGGAATC CTGGGGGGTC AGGGAAAGAT GCCCACCGTT 
GTTTCTCCTG AGCTGTACCG AGCTCGGTTC TGTGAGGCCA TGGACAAATA CTTCCTGATG 
GTACCCGACC ACTGGACGGG TCTTGGCATC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832602.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688462.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X6, mRNA.

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CloneID	OTo40925
Clone ID Related Accession (Same CDS sequence)	XM_029832602.1
Accession Version	XM_029832602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAGTA GGCGTCCAGA CGCAGACCTA ACAGACAAAC AGAAGAAGCA GGCCCTGAAT 
CCCCACATTG AGCTACAGTT CTCTGATGCA AGCGCAAAGT TTTACTGTCG GATCTACTAC 
GCCGAGGAGT TTCATAAGAT GCGTGAGGAG ATCATGGAGA GCTCCGAAGA GGACTTTGTG 
CGTTCCCTGT CGCACTGTGT CAACTGGCAG GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC 
TACGCAACAG AAGACGACCG TTTCATCCTA AAACAGATGC CCAGGCTGGA GGTGCAGTCC 
TTCTTGGACT TTGCTCCGCA CTACTTCACC TACATCACCG GAGCTGTGCA GCAGAAGAGG 
CCAACTGCAT TGGCAAAGAT TCTCGGAGTT TACAGGATAG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAACTGGA CCTGCTCGTC ATGGAAAACC TGTTCTACGG ACGCAAGATG 
GCTCAGGTCT TTGACCTGAA AGGTTCCCTC AGAAACCGTC ATGTGAAGAC AGACCTGGTA 
AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT GAGAATCTAC TGAAGCTGAT CCACGACAAT 
CCGCTCTACA TTCGCTCTCA CTGCAAGTCC ATCTTGCGCG CCGCCATACA CAGCGACGCC 
TACTTCCTGT CCAGCCACCT CATCATTGAC TACTCCCTGC TGGTGGGGTG GGACGATGCC 
ACCGACCAGC TGGTGGTTGG GATCATAGAC TATATTCGGA CGTTTACCTG GGACAAGAAA 
TTAGAGATGG TTGTCAAATC CACTGGAATC CTGGGGGGTC AGGGAAAGAT GCCCACCGTT 
GTTTCTCCTG AGCTGTACCG AGCTCGGTTC TGTGAGGCCA TGGACAAATA CTTCCTGATG 
GTACCCGACC ACTGGACGGG TCTTGGCATC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688462.1
CDS	134..6472
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASGESRVRGSPARPSESASSQQSRSSSEADPLSRRPDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832602.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832602.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAGTA GGCGTCCAGA CGCAGACCTA ACAGACAAAC AGAAGAAGCA GGCCCTGAAT 
CCCCACATTG AGCTACAGTT CTCTGATGCA AGCGCAAAGT TTTACTGTCG GATCTACTAC 
GCCGAGGAGT TTCATAAGAT GCGTGAGGAG ATCATGGAGA GCTCCGAAGA GGACTTTGTG 
CGTTCCCTGT CGCACTGTGT CAACTGGCAG GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC 
TACGCAACAG AAGACGACCG TTTCATCCTA AAACAGATGC CCAGGCTGGA GGTGCAGTCC 
TTCTTGGACT TTGCTCCGCA CTACTTCACC TACATCACCG GAGCTGTGCA GCAGAAGAGG 
CCAACTGCAT TGGCAAAGAT TCTCGGAGTT TACAGGATAG GCTACAAGAA CTCCCAGAAC 
AACACTGAGA AGAAACTGGA CCTGCTCGTC ATGGAAAACC TGTTCTACGG ACGCAAGATG 
GCTCAGGTCT TTGACCTGAA AGGTTCCCTC AGAAACCGTC ATGTGAAGAC AGACCTGGTA 
AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT GAGAATCTAC TGAAGCTGAT CCACGACAAT 
CCGCTCTACA TTCGCTCTCA CTGCAAGTCC ATCTTGCGCG CCGCCATACA CAGCGACGCC 
TACTTCCTGT CCAGCCACCT CATCATTGAC TACTCCCTGC TGGTGGGGTG GGACGATGCC 
ACCGACCAGC TGGTGGTTGG GATCATAGAC TATATTCGGA CGTTTACCTG GGACAAGAAA 
TTAGAGATGG TTGTCAAATC CACTGGAATC CTGGGGGGTC AGGGAAAGAT GCCCACCGTT 
GTTTCTCCTG AGCTGTACCG AGCTCGGTTC TGTGAGGCCA TGGACAAATA CTTCCTGATG 
GTACCCGACC ACTGGACGGG TCTTGGCATC AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832606.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688466.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X7, mRNA.

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CloneID	OTo40926
Clone ID Related Accession (Same CDS sequence)	XM_029832606.1
Accession Version	XM_029832606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA AAGACGCAGA CCTAACAGAC AAACAGAAGA AGCAGGCCCT GAATCCCCAC 
ATTGAGCTAC AGTTCTCTGA TGCAAGCGCA AAGTTTTACT GTCGGATCTA CTACGCCGAG 
GAGTTTCATA AGATGCGTGA GGAGATCATG GAGAGCTCCG AAGAGGACTT TGTGCGTTCC 
CTGTCGCACT GTGTCAACTG GCAGGCGCGC GGTGGAAAGT CTGGGGCCGT TTTCTACGCA 
ACAGAAGACG ACCGTTTCAT CCTAAAACAG ATGCCCAGGC TGGAGGTGCA GTCCTTCTTG 
GACTTTGCTC CGCACTACTT CACCTACATC ACCGGAGCTG TGCAGCAGAA GAGGCCAACT 
GCATTGGCAA AGATTCTCGG AGTTTACAGG ATAGGCTACA AGAACTCCCA GAACAACACT 
GAGAAGAAAC TGGACCTGCT CGTCATGGAA AACCTGTTCT ACGGACGCAA GATGGCTCAG 
GTCTTTGACC TGAAAGGTTC CCTCAGAAAC CGTCATGTGA AGACAGACCT GGTAAAGGAA 
AGCTGCGAGG TGGTCCTGCT GGATGAGAAT CTACTGAAGC TGATCCACGA CAATCCGCTC 
TACATTCGCT CTCACTGCAA GTCCATCTTG CGCGCCGCCA TACACAGCGA CGCCTACTTC 
CTGTCCAGCC ACCTCATCAT TGACTACTCC CTGCTGGTGG GGTGGGACGA TGCCACCGAC 
CAGCTGGTGG TTGGGATCAT AGACTATATT CGGACGTTTA CCTGGGACAA GAAATTAGAG 
ATGGTTGTCA AATCCACTGG AATCCTGGGG GGTCAGGGAA AGATGCCCAC CGTTGTTTCT 
CCTGAGCTGT ACCGAGCTCG GTTCTGTGAG GCCATGGACA AATACTTCCT GATGGTACCC 
GACCACTGGA CGGGTCTTGG CATCAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688466.1
CDS	129..6461
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832606.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832606.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA AAGACGCAGA CCTAACAGAC AAACAGAAGA AGCAGGCCCT GAATCCCCAC 
ATTGAGCTAC AGTTCTCTGA TGCAAGCGCA AAGTTTTACT GTCGGATCTA CTACGCCGAG 
GAGTTTCATA AGATGCGTGA GGAGATCATG GAGAGCTCCG AAGAGGACTT TGTGCGTTCC 
CTGTCGCACT GTGTCAACTG GCAGGCGCGC GGTGGAAAGT CTGGGGCCGT TTTCTACGCA 
ACAGAAGACG ACCGTTTCAT CCTAAAACAG ATGCCCAGGC TGGAGGTGCA GTCCTTCTTG 
GACTTTGCTC CGCACTACTT CACCTACATC ACCGGAGCTG TGCAGCAGAA GAGGCCAACT 
GCATTGGCAA AGATTCTCGG AGTTTACAGG ATAGGCTACA AGAACTCCCA GAACAACACT 
GAGAAGAAAC TGGACCTGCT CGTCATGGAA AACCTGTTCT ACGGACGCAA GATGGCTCAG 
GTCTTTGACC TGAAAGGTTC CCTCAGAAAC CGTCATGTGA AGACAGACCT GGTAAAGGAA 
AGCTGCGAGG TGGTCCTGCT GGATGAGAAT CTACTGAAGC TGATCCACGA CAATCCGCTC 
TACATTCGCT CTCACTGCAA GTCCATCTTG CGCGCCGCCA TACACAGCGA CGCCTACTTC 
CTGTCCAGCC ACCTCATCAT TGACTACTCC CTGCTGGTGG GGTGGGACGA TGCCACCGAC 
CAGCTGGTGG TTGGGATCAT AGACTATATT CGGACGTTTA CCTGGGACAA GAAATTAGAG 
ATGGTTGTCA AATCCACTGG AATCCTGGGG GGTCAGGGAA AGATGCCCAC CGTTGTTTCT 
CCTGAGCTGT ACCGAGCTCG GTTCTGTGAG GCCATGGACA AATACTTCCT GATGGTACCC 
GACCACTGGA CGGGTCTTGG CATCAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832611.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688471.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X8, mRNA.

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CloneID	OTo40927
Clone ID Related Accession (Same CDS sequence)	XM_029832611.1
Accession Version	XM_029832611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA ACGCAGACCT AACAGACAAA CAGAAGAAGC AGGCCCTGAA TCCCCACATT 
GAGCTACAGT TCTCTGATGC AAGCGCAAAG TTTTACTGTC GGATCTACTA CGCCGAGGAG 
TTTCATAAGA TGCGTGAGGA GATCATGGAG AGCTCCGAAG AGGACTTTGT GCGTTCCCTG 
TCGCACTGTG TCAACTGGCA GGCGCGCGGT GGAAAGTCTG GGGCCGTTTT CTACGCAACA 
GAAGACGACC GTTTCATCCT AAAACAGATG CCCAGGCTGG AGGTGCAGTC CTTCTTGGAC 
TTTGCTCCGC ACTACTTCAC CTACATCACC GGAGCTGTGC AGCAGAAGAG GCCAACTGCA 
TTGGCAAAGA TTCTCGGAGT TTACAGGATA GGCTACAAGA ACTCCCAGAA CAACACTGAG 
AAGAAACTGG ACCTGCTCGT CATGGAAAAC CTGTTCTACG GACGCAAGAT GGCTCAGGTC 
TTTGACCTGA AAGGTTCCCT CAGAAACCGT CATGTGAAGA CAGACCTGGT AAAGGAAAGC 
TGCGAGGTGG TCCTGCTGGA TGAGAATCTA CTGAAGCTGA TCCACGACAA TCCGCTCTAC 
ATTCGCTCTC ACTGCAAGTC CATCTTGCGC GCCGCCATAC ACAGCGACGC CTACTTCCTG 
TCCAGCCACC TCATCATTGA CTACTCCCTG CTGGTGGGGT GGGACGATGC CACCGACCAG 
CTGGTGGTTG GGATCATAGA CTATATTCGG ACGTTTACCT GGGACAAGAA ATTAGAGATG 
GTTGTCAAAT CCACTGGAAT CCTGGGGGGT CAGGGAAAGA TGCCCACCGT TGTTTCTCCT 
GAGCTGTACC GAGCTCGGTT CTGTGAGGCC ATGGACAAAT ACTTCCTGAT GGTACCCGAC 
CACTGGACGG GTCTTGGCAT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688471.1
CDS	131..6460
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLNADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832611.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832611.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTAGTG GAGAGAGTCG GGTGAGGGGC 
AGCCCCGCCA GGCCCAGTGA GTCAGCCTCG TCCCAACAGA GCCGCAGCAG CAGTGAAGCA 
GATCCCCTCA ACGCAGACCT AACAGACAAA CAGAAGAAGC AGGCCCTGAA TCCCCACATT 
GAGCTACAGT TCTCTGATGC AAGCGCAAAG TTTTACTGTC GGATCTACTA CGCCGAGGAG 
TTTCATAAGA TGCGTGAGGA GATCATGGAG AGCTCCGAAG AGGACTTTGT GCGTTCCCTG 
TCGCACTGTG TCAACTGGCA GGCGCGCGGT GGAAAGTCTG GGGCCGTTTT CTACGCAACA 
GAAGACGACC GTTTCATCCT AAAACAGATG CCCAGGCTGG AGGTGCAGTC CTTCTTGGAC 
TTTGCTCCGC ACTACTTCAC CTACATCACC GGAGCTGTGC AGCAGAAGAG GCCAACTGCA 
TTGGCAAAGA TTCTCGGAGT TTACAGGATA GGCTACAAGA ACTCCCAGAA CAACACTGAG 
AAGAAACTGG ACCTGCTCGT CATGGAAAAC CTGTTCTACG GACGCAAGAT GGCTCAGGTC 
TTTGACCTGA AAGGTTCCCT CAGAAACCGT CATGTGAAGA CAGACCTGGT AAAGGAAAGC 
TGCGAGGTGG TCCTGCTGGA TGAGAATCTA CTGAAGCTGA TCCACGACAA TCCGCTCTAC 
ATTCGCTCTC ACTGCAAGTC CATCTTGCGC GCCGCCATAC ACAGCGACGC CTACTTCCTG 
TCCAGCCACC TCATCATTGA CTACTCCCTG CTGGTGGGGT GGGACGATGC CACCGACCAG 
CTGGTGGTTG GGATCATAGA CTATATTCGG ACGTTTACCT GGGACAAGAA ATTAGAGATG 
GTTGTCAAAT CCACTGGAAT CCTGGGGGGT CAGGGAAAGA TGCCCACCGT TGTTTCTCCT 
GAGCTGTACC GAGCTCGGTT CTGTGAGGCC ATGGACAAAT ACTTCCTGAT GGTACCCGAC 
CACTGGACGG GTCTTGGCAT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832613.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688473.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X9, mRNA.

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CloneID	OTo40928
Clone ID Related Accession (Same CDS sequence)	XM_029832613.1
Accession Version	XM_029832613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAAAG ACGCAGACCT AACAGACAAA CAGAAGAAGC AGGCCCTGAA TCCCCACATT 
GAGCTACAGT TCTCTGATGC AAGCGCAAAG TTTTACTGTC GGATCTACTA CGCCGAGGAG 
TTTCATAAGA TGCGTGAGGA GATCATGGAG AGCTCCGAAG AGGACTTTGT GCGTTCCCTG 
TCGCACTGTG TCAACTGGCA GGCGCGCGGT GGAAAGTCTG GGGCCGTTTT CTACGCAACA 
GAAGACGACC GTTTCATCCT AAAACAGATG CCCAGGCTGG AGGTGCAGTC CTTCTTGGAC 
TTTGCTCCGC ACTACTTCAC CTACATCACC GGAGCTGTGC AGCAGAAGAG GCCAACTGCA 
TTGGCAAAGA TTCTCGGAGT TTACAGGATA GGCTACAAGA ACTCCCAGAA CAACACTGAG 
AAGAAACTGG ACCTGCTCGT CATGGAAAAC CTGTTCTACG GACGCAAGAT GGCTCAGGTC 
TTTGACCTGA AAGGTTCCCT CAGAAACCGT CATGTGAAGA CAGACCTGGT AAAGGAAAGC 
TGCGAGGTGG TCCTGCTGGA TGAGAATCTA CTGAAGCTGA TCCACGACAA TCCGCTCTAC 
ATTCGCTCTC ACTGCAAGTC CATCTTGCGC GCCGCCATAC ACAGCGACGC CTACTTCCTG 
TCCAGCCACC TCATCATTGA CTACTCCCTG CTGGTGGGGT GGGACGATGC CACCGACCAG 
CTGGTGGTTG GGATCATAGA CTATATTCGG ACGTTTACCT GGGACAAGAA ATTAGAGATG 
GTTGTCAAAT CCACTGGAAT CCTGGGGGGT CAGGGAAAGA TGCCCACCGT TGTTTCTCCT 
GAGCTGTACC GAGCTCGGTT CTGTGAGGCC ATGGACAAAT ACTTCCTGAT GGTACCCGAC 
CACTGGACGG GTCTTGGCAT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688473.1
CDS	132..6461
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832613.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832613.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAAAG ACGCAGACCT AACAGACAAA CAGAAGAAGC AGGCCCTGAA TCCCCACATT 
GAGCTACAGT TCTCTGATGC AAGCGCAAAG TTTTACTGTC GGATCTACTA CGCCGAGGAG 
TTTCATAAGA TGCGTGAGGA GATCATGGAG AGCTCCGAAG AGGACTTTGT GCGTTCCCTG 
TCGCACTGTG TCAACTGGCA GGCGCGCGGT GGAAAGTCTG GGGCCGTTTT CTACGCAACA 
GAAGACGACC GTTTCATCCT AAAACAGATG CCCAGGCTGG AGGTGCAGTC CTTCTTGGAC 
TTTGCTCCGC ACTACTTCAC CTACATCACC GGAGCTGTGC AGCAGAAGAG GCCAACTGCA 
TTGGCAAAGA TTCTCGGAGT TTACAGGATA GGCTACAAGA ACTCCCAGAA CAACACTGAG 
AAGAAACTGG ACCTGCTCGT CATGGAAAAC CTGTTCTACG GACGCAAGAT GGCTCAGGTC 
TTTGACCTGA AAGGTTCCCT CAGAAACCGT CATGTGAAGA CAGACCTGGT AAAGGAAAGC 
TGCGAGGTGG TCCTGCTGGA TGAGAATCTA CTGAAGCTGA TCCACGACAA TCCGCTCTAC 
ATTCGCTCTC ACTGCAAGTC CATCTTGCGC GCCGCCATAC ACAGCGACGC CTACTTCCTG 
TCCAGCCACC TCATCATTGA CTACTCCCTG CTGGTGGGGT GGGACGATGC CACCGACCAG 
CTGGTGGTTG GGATCATAGA CTATATTCGG ACGTTTACCT GGGACAAGAA ATTAGAGATG 
GTTGTCAAAT CCACTGGAAT CCTGGGGGGT CAGGGAAAGA TGCCCACCGT TGTTTCTCCT 
GAGCTGTACC GAGCTCGGTT CTGTGAGGCC ATGGACAAAT ACTTCCTGAT GGTACCCGAC 
CACTGGACGG GTCTTGGCAT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832622.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688482.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X10, mRNA.

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CloneID	OTo40929
Clone ID Related Accession (Same CDS sequence)	XM_029832622.1
Accession Version	XM_029832622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAACG CAGACCTAAC AGACAAACAG AAGAAGCAGG CCCTGAATCC CCACATTGAG 
CTACAGTTCT CTGATGCAAG CGCAAAGTTT TACTGTCGGA TCTACTACGC CGAGGAGTTT 
CATAAGATGC GTGAGGAGAT CATGGAGAGC TCCGAAGAGG ACTTTGTGCG TTCCCTGTCG 
CACTGTGTCA ACTGGCAGGC GCGCGGTGGA AAGTCTGGGG CCGTTTTCTA CGCAACAGAA 
GACGACCGTT TCATCCTAAA ACAGATGCCC AGGCTGGAGG TGCAGTCCTT CTTGGACTTT 
GCTCCGCACT ACTTCACCTA CATCACCGGA GCTGTGCAGC AGAAGAGGCC AACTGCATTG 
GCAAAGATTC TCGGAGTTTA CAGGATAGGC TACAAGAACT CCCAGAACAA CACTGAGAAG 
AAACTGGACC TGCTCGTCAT GGAAAACCTG TTCTACGGAC GCAAGATGGC TCAGGTCTTT 
GACCTGAAAG GTTCCCTCAG AAACCGTCAT GTGAAGACAG ACCTGGTAAA GGAAAGCTGC 
GAGGTGGTCC TGCTGGATGA GAATCTACTG AAGCTGATCC ACGACAATCC GCTCTACATT 
CGCTCTCACT GCAAGTCCAT CTTGCGCGCC GCCATACACA GCGACGCCTA CTTCCTGTCC 
AGCCACCTCA TCATTGACTA CTCCCTGCTG GTGGGGTGGG ACGATGCCAC CGACCAGCTG 
GTGGTTGGGA TCATAGACTA TATTCGGACG TTTACCTGGG ACAAGAAATT AGAGATGGTT 
GTCAAATCCA CTGGAATCCT GGGGGGTCAG GGAAAGATGC CCACCGTTGT TTCTCCTGAG 
CTGTACCGAG CTCGGTTCTG TGAGGCCATG GACAAATACT TCCTGATGGT ACCCGACCAC 
TGGACGGGTC TTGGCATCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688482.1
CDS	134..6460
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASGESRVRGSPARPSESASSQQSRSSSEADPLNADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832622.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832622.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGAAAAACAC CTATTGGGAT GAGAAAGAAC 
ATGATTCACC AGGAACCTCA GAGCAGCGGC CTCAGTTCCA GACTGACAGG ACTTCAGGAG 
GATGTAGGAA AATCACCTGG TAGGAGGAGG TCTGCTAGCA TCAGCACACT TCCACTTGAC 
CGCTCTGGGT CATCAATGGT ACCTTCCTAC GACAGCTCCG TCAGCCCCCC TACCAGCAGA 
GCCATGTCGG TGTCAAAGGC TGGGACAAAA TTAGATCACA GTGAGGAGGA ACGTAAAATA 
CTACTGGACT CCTCCCAGTT GAAGGACTTA TGGAGGAAAA TCTGTCATAA TGGAACAGGG 
ATGGAATTCC AGGACCACAG ATACTGGCTG AGAACCTACC CCAACTGCAT TGTGGGAAAA 
GAGCTCGTCA ACTGGCTGCT AAGAAATGGC ACCATTTCCA ACAGGGTCCA AGCTATTGCC 
ATAGGTCAGG CACTCGTGGA TGGCCGCTGG CTCGACTGTG TCACCCACCA CGATCAGCTA 
TTCAGAGATG AGTATGCTCT CTATCGACCA CTTCAGAGTA CAGAGTTCTC AGAAACTCCC 
TCCCCGGACA GCGATAGTGT CAACTCACTA GAGGGACATT CAGAGCCGTC ATGGTTCAAG 
GACATCAAAT TCGATGACAG CGACACCGAG CAGCCCGCAG ACGACACAGA GTACACCATG 
CCTAGTTCCG CTGGGCCCAG CAAGAGGACA TCTGTCAGCA GCGTCCACTC AGCAGTAGAC 
AGTGACTCAG CTGCCTCCAT CAACCTCAAC ATGGAGCAAA ACAATGTGAA TTTTCGAATC 
AAGAAAGATT CCAAGTGTCC CCAGGCATTC CCCGCCCTGG AGCACAAATC TGAAATTATG 
GCGTCTGAAG ATGGAGGAGA GAACATCCTT ATAAGTGATG CCTTTATCAA AGAGTCTCTC 
TTTAACCGTC GTGTGGAGGA GAAGGCTAAA GAAATGCTGT TTACTCCACT GGGTTGGCAC 
CACAGCTCTC TGGACCAGCT CAGAGAGGAG AATGGAGAAA AGAAGGCTAT GGAGAGGCTG 
CTCTCGGCAA ATCACAGCCA CATGATGGCG CTGCTGCAGC AGTTGCTCTA CAGCGAGTCT 
TTGTCTCTGT CCTGGCGGGA TATTATCGTT CCTGTGGTTC GCCAGGTTGT CCAGACAGTT 
CGACCTGATG TTCGCAACAG TGATGACGAC ATGGACATCC GCCAACTGGT CCATATCAAA 
AAGATTCCTG GAGGAAAAAA GTTTGACTCA ACTGTAGTGA ATGGCTTTGT GTGTACGAAG 
AGTATTGCCC ACAAAAAGAT GAACCCATAT ATCAAGAACC CCAAAATTCT GCTGCTGAAA 
TGCTCAATAG AGTATCTGTA CAGGGAAGAG ACCAAATTCA CGTCCATCGA CCCCATCGTG 
CTGCAGGAAC GAGAGTTTCT GAAAAACTAT GTGCAACGTA TCGTGGATGT TCGGCCCACT 
CTGGTTCTGG TGGAAAAAAC GGTGTCTCGT ATAGCTCAGG ACATGCTGCT AGAACACGGC 
ATTACCCTGG TCATTAATGT CAAACCGCAA GTATTGGACA GGGTGAGTTG TGTGACCCAG 
GGGGACCTTG TCATGTCCAT GGACCAGCTA CTCACCAAAC CTCGTCTGGG TACCTGCCAC 
AAGTTTTACA TGCAGCCCTT CCCTTTGGCC AATAATGAGG TCAAGACTCT GATGTTCTTC 
GAAGGATGTC CTGCTCATCT CGGGTGCTCC ATAAATCTAC GTGGTGCTTC AGACTACGAG 
TTAGCCAGGG TGAAGGAGAT CATGCGATTA ATGGTGTGTG TGGCCTACCA CTCTCAGCTG 
GAGATCTCCT TCCTCATGGA TGAGTTTGCC ATGCCGCCCA GCTTGTCCCA GAGCACTTCA 
TTTCCCTGTC TCCTCGACGG AGGCTCTGCA GATGAGGAAG CAAGCAGGGA GAAACTAGAG 
AAGAGTTCTG AAGATGAAGA CACTGTAGTG GAAGGACAAC CTGAGGAGGT GCCCTCTGCC 
AATGATGTAG ACACTTCCCA GGACACAAAT GATCCTAAAT TATTCTCCTC TATGGTGGAA 
GAGACCACTA ATACTGACAC AGTCACGACA AAGTCTTCCA CTCCGTTCCA AAGCCCTCGT 
GCGCCATGCT TGCCAGTTCC TCCACCATTC ATCATGGAAG AAACCCCAGA AATGGGCTCA 
GACAGCCTAA CAGGGGCATT TGAGGCAGGG GTGGTGGAAT GCTGCCCAAA GGAGGAATCA 
ACCAACCTAG AAGATGGAGA AGATTCTGAT ATTCCTACTC CTAAGTTGTT CCGAGACCCC 
CTGCAAGATG ATACTGGGAT GTTTGTGGCT GAAGAAGTTG CTTCTTCAGA TGACCATCTC 
AAGTCCATCT CTGCTATCTT CAAACAGGAA CTTAAGGACA TCATCCTGTG CATTTCACCT 
TTCATCACAT TTGAGAAACC ATTTCTTCTT ACCCCTGCTG GGATGCACTG CCCGAGCAGG 
GATTATTTTC CCGAACAGGT CTATCTGTCT CCTCTCCTCA ACAAGGACTT TAAAGAGTTG 
GATGGACGCA GAAAGCGGCA GCTCCTTAAG GAGTCCACTC CCTCCCTGGC AAACAGCCTA 
ACGAATGGTG GCCCCCCGTC AAAGCATGTC AGTGTCCTGC CATCCCACAG TCTCACCAGC 
ACCCGGATCG TTGAACTAGT AAACAGGAGC CAGGGTCTGG CCAGAATGGT TGCAGACTAC 
AGAGCAAAGG GTGGACGTAT CCAGCACAGC GAAGCTACTG ATCCATTTTG CATAAGCAGC 
TCTTCTGCTC CGGTCAGTGG CCAGAATAGA CCAGGTGATG GTTCGGTCAG AGCACCAGTA 
AAGACTGACA GCGAAGAGGA GAAGCCAACT AAACCCAGTG ACTGGACCCC CAAGCTGGAT 
TGTCTGAACC CCACCAACCA CCAGAGACTC TGTGTGCTTT TTAGCAGTTC ATCAACTCAG 
TCCAGCAATG CCCCCAACCC CTGTGTCAAC CCATGGATTG TTACCATGGA GTTTTATGGC 
AAGAATGACC TCTGCCTTGG TGTATTCTTG GAGCGATATT GCTTTAGGCC ATCATACCAG 
TGCCCCAGTA TATTTTGTGA GACCCCCATG GTGCACCACA TCCGGCGTTT TGTCCATGGC 
AACGGCTGCG TGCAGATAGT ACTGAAGGAG CTGGACTCCC CTGTGCCGGG GTACCAGCAC 
ACCATTCTCA ACTACTCCTG GTGCCGCATC TGCAAACAAG TGACACCAGT GGTTCCTCTA 
TCCAATGACT CATGGTCAAT GTCTTTTGCA AAATATCTGG AGCTTCGCTT CTACGGCCAC 
CAGTACACCA GGAGGGCCAA TGCAGAGCCC TGTGGCCACT CCATCCACAA AGACTACCAC 
CAGTACTTCT CATACAACCA AATGGTGGCT TCCTTCAGTT ACACCTCAGT GAGGCTGCTG 
GAGATTTGTC TTCCTCGTTT CAAAATCTTC ATCAGGAATC AGGGTCCTTC GATCAACAAC 
TTGGGGCAGG ATCTGAAAGA CTTCTCTCAA AAAGTAAATC AGGTTTACGT GGCCATAGAT 
GATCGTCTCA CATCTCTAAA GACGGACACC TTCAGTAAAA CACGCGAGGA GAAGATGGAG 
GACCTCTTTG CACAGAAAGA TATGGAAGAG GCGGAACTGC GCAGCTGGAT TGAAAAGTTT 
CAAGCACGAT TGCAGGCTTG TGGTCACGTT TCTCCTCAGC AGCTGCAGAC GACTCTGGAA 
TCACTGGTGA TGAAGAAACA GAGTCTGTGT GAGATGTTGC AGTACTGGAA CAGCAGGCTG 
CAGGAATTAT TCCAACAGGA GAAAGGCAGG AAACGTCTGT CAGTGCCCCC AAGCCCCGGC 
AGGCACAGAC AGACTGCCCC TGATGATGGC AAGAACGCTG TGGAGTCATC CCCACGTAAC 
CCTTCACCTG TGGTACAGAA TGGTGAAAAG GAGGACCGTC ACCTCACCAC ACTGCCTTCA 
ACCTCCTCTC TGTTGCCCTC GCCTGGAGAC CTGGGAGCTG AACCAGCCAC ATCTGGTCCT 
TCCCTGAGTG AGCAGGACTC TGTCAGCATC CCAGAAGATG TATTTGATGG ACACTTGCTG 
GGCTCCAATG ACAGTCAGGT GAAGGAGAAA TCCACCATGA AAGCCATCCT TGCCAACTTC 
CTGCCCGGCA ACAGTTACAA CCCCATTCCA TTCCCATTTG ACCCAGACAA ACACTACCTG 
ATGGATGAGT ATGAGCGAGT GCCAATTGCA GTGTGTGAAA GAGAACCAAG CTCCATCATT 
GCATTTGCTC TGAGTTGTAA GAAGTATAAA ACAGCACTGG AAGATCTGTC CAAGGTGTCA 
AACATGGGTG GAGAAGACTC TTCACAAGCT GCCAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAACG CAGACCTAAC AGACAAACAG AAGAAGCAGG CCCTGAATCC CCACATTGAG 
CTACAGTTCT CTGATGCAAG CGCAAAGTTT TACTGTCGGA TCTACTACGC CGAGGAGTTT 
CATAAGATGC GTGAGGAGAT CATGGAGAGC TCCGAAGAGG ACTTTGTGCG TTCCCTGTCG 
CACTGTGTCA ACTGGCAGGC GCGCGGTGGA AAGTCTGGGG CCGTTTTCTA CGCAACAGAA 
GACGACCGTT TCATCCTAAA ACAGATGCCC AGGCTGGAGG TGCAGTCCTT CTTGGACTTT 
GCTCCGCACT ACTTCACCTA CATCACCGGA GCTGTGCAGC AGAAGAGGCC AACTGCATTG 
GCAAAGATTC TCGGAGTTTA CAGGATAGGC TACAAGAACT CCCAGAACAA CACTGAGAAG 
AAACTGGACC TGCTCGTCAT GGAAAACCTG TTCTACGGAC GCAAGATGGC TCAGGTCTTT 
GACCTGAAAG GTTCCCTCAG AAACCGTCAT GTGAAGACAG ACCTGGTAAA GGAAAGCTGC 
GAGGTGGTCC TGCTGGATGA GAATCTACTG AAGCTGATCC ACGACAATCC GCTCTACATT 
CGCTCTCACT GCAAGTCCAT CTTGCGCGCC GCCATACACA GCGACGCCTA CTTCCTGTCC 
AGCCACCTCA TCATTGACTA CTCCCTGCTG GTGGGGTGGG ACGATGCCAC CGACCAGCTG 
GTGGTTGGGA TCATAGACTA TATTCGGACG TTTACCTGGG ACAAGAAATT AGAGATGGTT 
GTCAAATCCA CTGGAATCCT GGGGGGTCAG GGAAAGATGC CCACCGTTGT TTCTCCTGAG 
CTGTACCGAG CTCGGTTCTG TGAGGCCATG GACAAATACT TCCTGATGGT ACCCGACCAC 
TGGACGGGTC TTGGCATCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607487.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605789.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X11, mRNA.

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CloneID	OTo40930
Clone ID Related Accession (Same CDS sequence)	XM_011607487.2 , XM_011607495.2
Accession Version	XM_011607487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011607487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA GTTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605789.1
CDS	157..6477
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607487.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607487.2

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA GTTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607495.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605797.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X12, mRNA.

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CloneID	OTo40930
Clone ID Related Accession (Same CDS sequence)	XM_011607487.2 , XM_011607495.2
Accession Version	XM_011607495.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011607495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA GTTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605797.1
CDS	150..6470
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607495.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607495.2

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA GTTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832636.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688496.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X13, mRNA.

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CloneID	OTo40931
Clone ID Related Accession (Same CDS sequence)	XM_029832636.1
Accession Version	XM_029832636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGCATGATTC ACCAGGAACC TCAGAGCAGC 
GGCCTCAGTT CCAGACTGAC AGGACTTCAG GAGGATGTAG GAAAATCACC TGGTAGGAGG 
AGGTCTGCTA GCATCAGCAC ACTTCCACTT GACCGCTCTG GGTCATCAAT GGTACCTTCC 
TACGACAGCT CCGTCAGCCC CCCTACCAGC AGAGCCATGT CGGTGTCAAA GGCTGGGACA 
AAATTAGATC ACAGTGAGGA GGAACGTAAA ATACTACTGG ACTCCTCCCA GTTGAAGGAC 
TTATGGAGGA AAATCTGTCA TAATGGAACA GGGATGGAAT TCCAGGACCA CAGATACTGG 
CTGAGAACCT ACCCCAACTG CATTGTGGGA AAAGAGCTCG TCAACTGGCT GCTAAGAAAT 
GGCACCATTT CCAACAGGGT CCAAGCTATT GCCATAGGTC AGGCACTCGT GGATGGCCGC 
TGGCTCGACT GTGTCACCCA CCACGATCAG CTATTCAGAG ATGAGTATGC TCTCTATCGA 
CCACTTCAGA GTACAGAGTT CTCAGAAACT CCCTCCCCGG ACAGCGATAG TGTCAACTCA 
CTAGAGGGAC ATTCAGAGCC GTCATGGTTC AAGGACATCA AATTCGATGA CAGCGACACC 
GAGCAGCCCG CAGACGACAC AGAGTACACC ATGCCTAGTT CCGCTGGGCC CAGCAAGAGG 
ACATCTGTCA GCAGCGTCCA CTCAGCAGTA GACAGTGACT CAGCTGCCTC CATCAACCTC 
AACATGGAGC AAAACAATGT GAATTTTCGA ATCAAGAAAG ATTCCAAGTG TCCCCAGGCA 
TTCCCCGCCC TGGAGCACAA ATCTGAAATT ATGGCGTCTG AAGATGGAGG AGAGAACATC 
CTTATAAGTG ATGCCTTTAT CAAAGAGTCT CTCTTTAACC GTCGTGTGGA GGAGAAGGCT 
AAAGAAATGC TGTTTACTCC ACTGGGTTGG CACCACAGCT CTCTGGACCA GCTCAGAGAG 
GAGAATGGAG AAAAGAAGGC TATGGAGAGG CTGCTCTCGG CAAATCACAG CCACATGATG 
GCGCTGCTGC AGCAGTTGCT CTACAGCGAG TCTTTGTCTC TGTCCTGGCG GGATATTATC 
GTTCCTGTGG TTCGCCAGGT TGTCCAGACA GTTCGACCTG ATGTTCGCAA CAGTGATGAC 
GACATGGACA TCCGCCAACT GGTCCATATC AAAAAGATTC CTGGAGGAAA AAAGTTTGAC 
TCAACTGTAG TGAATGGCTT TGTGTGTACG AAGAGTATTG CCCACAAAAA GATGAACCCA 
TATATCAAGA ACCCCAAAAT TCTGCTGCTG AAATGCTCAA TAGAGTATCT GTACAGGGAA 
GAGACCAAAT TCACGTCCAT CGACCCCATC GTGCTGCAGG AACGAGAGTT TCTGAAAAAC 
TATGTGCAAC GTATCGTGGA TGTTCGGCCC ACTCTGGTTC TGGTGGAAAA AACGGTGTCT 
CGTATAGCTC AGGACATGCT GCTAGAACAC GGCATTACCC TGGTCATTAA TGTCAAACCG 
CAAGTATTGG ACAGGGTGAG TTGTGTGACC CAGGGGGACC TTGTCATGTC CATGGACCAG 
CTACTCACCA AACCTCGTCT GGGTACCTGC CACAAGTTTT ACATGCAGCC CTTCCCTTTG 
GCCAATAATG AGGTCAAGAC TCTGATGTTC TTCGAAGGAT GTCCTGCTCA TCTCGGGTGC 
TCCATAAATC TACGTGGTGC TTCAGACTAC GAGTTAGCCA GGGTGAAGGA GATCATGCGA 
TTAATGGTGT GTGTGGCCTA CCACTCTCAG CTGGAGATCT CCTTCCTCAT GGATGAGTTT 
GCCATGCCGC CCAGCTTGTC CCAGAGCACT TCATTTCCCT GTCTCCTCGA CGGAGGCTCT 
GCAGATGAGG AAGCAAGCAG GGAGAAACTA GAGAAGAGTT CTGAAGATGA AGACACTGTA 
GTGGAAGGAC AACCTGAGGA GGTGCCCTCT GCCAATGATG TAGACACTTC CCAGGACACA 
AATGATCCTA AATTATTCTC CTCTATGGTG GAAGAGACCA CTAATACTGA CACAGTCACG 
ACAAAGTCTT CCACTCCGTT CCAAAGCCCT CGTGCGCCAT GCTTGCCAGT TCCTCCACCA 
TTCATCATGG AAGAAACCCC AGAAATGGGC TCAGACAGCC TAACAGGGGC ATTTGAGGCA 
GGGGTGGTGG AATGCTGCCC AAAGGAGGAA TCAACCAACC TAGAAGATGG AGAAGATTCT 
GATATTCCTA CTCCTAAGTT GTTCCGAGAC CCCCTGCAAG ATGATACTGG GATGTTTGTG 
GCTGAAGAAG TTGCTTCTTC AGATGACCAT CTCAAGTCCA TCTCTGCTAT CTTCAAACAG 
GAACTTAAGG ACATCATCCT GTGCATTTCA CCTTTCATCA CATTTGAGAA ACCATTTCTT 
CTTACCCCTG CTGGGATGCA CTGCCCGAGC AGGGATTATT TTCCCGAACA GGTCTATCTG 
TCTCCTCTCC TCAACAAGGA CTTTAAAGAG TTGGATGGAC GCAGAAAGCG GCAGCTCCTT 
AAGGAGTCCA CTCCCTCCCT GGCAAACAGC CTAACGAATG GTGGCCCCCC GTCAAAGCAT 
GTCAGTGTCC TGCCATCCCA CAGTCTCACC AGCACCCGGA TCGTTGAACT AGTAAACAGG 
AGCCAGGGTC TGGCCAGAAT GGTTGCAGAC TACAGAGCAA AGGGTGGACG TATCCAGCAC 
AGCGAAGCTA CTGATCCATT TTGCATAAGC AGCTCTTCTG CTCCGGTCAG TGGCCAGAAT 
AGACCAGGTG ATGGTTCGGT CAGAGCACCA GTAAAGACTG ACAGCGAAGA GGAGAAGCCA 
ACTAAACCCA GTGACTGGAC CCCCAAGCTG GATTGTCTGA ACCCCACCAA CCACCAGAGA 
CTCTGTGTGC TTTTTAGCAG TTCATCAACT CAGTCCAGCA ATGCCCCCAA CCCCTGTGTC 
AACCCATGGA TTGTTACCAT GGAGTTTTAT GGCAAGAATG ACCTCTGCCT TGGTGTATTC 
TTGGAGCGAT ATTGCTTTAG GCCATCATAC CAGTGCCCCA GTATATTTTG TGAGACCCCC 
ATGGTGCACC ACATCCGGCG TTTTGTCCAT GGCAACGGCT GCGTGCAGAT AGTACTGAAG 
GAGCTGGACT CCCCTGTGCC GGGGTACCAG CACACCATTC TCAACTACTC CTGGTGCCGC 
ATCTGCAAAC AAGTGACACC AGTGGTTCCT CTATCCAATG ACTCATGGTC AATGTCTTTT 
GCAAAATATC TGGAGCTTCG CTTCTACGGC CACCAGTACA CCAGGAGGGC CAATGCAGAG 
CCCTGTGGCC ACTCCATCCA CAAAGACTAC CACCAGTACT TCTCATACAA CCAAATGGTG 
GCTTCCTTCA GTTACACCTC AGTGAGGCTG CTGGAGATTT GTCTTCCTCG TTTCAAAATC 
TTCATCAGGA ATCAGGGTCC TTCGATCAAC AACTTGGGGC AGGATCTGAA AGACTTCTCT 
CAAAAAGTAA ATCAGGTTTA CGTGGCCATA GATGATCGTC TCACATCTCT AAAGACGGAC 
ACCTTCAGTA AAACACGCGA GGAGAAGATG GAGGACCTCT TTGCACAGAA AGATATGGAA 
GAGGCGGAAC TGCGCAGCTG GATTGAAAAG TTTCAAGCAC GATTGCAGGC TTGTGGTCAC 
GTTTCTCCTC AGCAGCTGCA GACGACTCTG GAATCACTGG TGATGAAGAA ACAGAGTCTG 
TGTGAGATGT TGCAGTACTG GAACAGCAGG CTGCAGGAAT TATTCCAACA GGAGAAAGGC 
AGGAAACGTC TGTCAGTGCC CCCAAGCCCC GGCAGGCACA GACAGACTGC CCCTGATGAT 
GGCAAGAACG CTGTGGAGTC ATCCCCACGT AACCCTTCAC CTGTGGTACA GAATGGTGAA 
AAGGAGGACC GTCACCTCAC CACACTGCCT TCAACCTCCT CTCTGTTGCC CTCGCCTGGA 
GACCTGGGAG CTGAACCAGC CACATCTGGT CCTTCCCTGA GTGAGCAGGA CTCTGTCAGC 
ATCCCAGAAG ATGTATTTGA TGGACACTTG CTGGGCTCCA ATGACAGTCA GGTGAAGGAG 
AAATCCACCA TGAAAGCCAT CCTTGCCAAC TTCCTGCCCG GCAACAGTTA CAACCCCATT 
CCATTCCCAT TTGACCCAGA CAAACACTAC CTGATGGATG AGTATGAGCG AGTGCCAATT 
GCAGTGTGTG AAAGAGAACC AAGCTCCATC ATTGCATTTG CTCTGAGTTG TAAGAAGTAT 
AAAACAGCAC TGGAAGATCT GTCCAAGGTG TCAAACATGG GTGGAGAAGA CTCTTCACAA 
GCTGCCAGTA GTGGAGAGAG TCGGGTGAGG GGCAGCCCCG CCAGGCCCAG TGAGTCAGCC 
TCGTCCCAAC AGAGCCGCAG CAGCAGTGAA GCAGATCCCC TCAGTAGGCG TCCAGAAGAC 
GCAGACCTAA CAGACAAACA GAAGAAGCAG GCCCTGAATC CCCACATTGA GCTACAGTTC 
TCTGATGCAA GCGCAAAGTT TTACTGTCGG ATCTACTACG CCGAGGAGTT TCATAAGATG 
CGTGAGGAGA TCATGGAGAG CTCCGAAGAG GACTTTGTGC GTTCCCTGTC GCACTGTGTC 
AACTGGCAGG CGCGCGGTGG AAAGTCTGGG GCCGTTTTCT ACGCAACAGA AGACGACCGT 
TTCATCCTAA AACAGATGCC CAGGCTGGAG GTGCAGTCCT TCTTGGACTT TGCTCCGCAC 
TACTTCACCT ACATCACCGG AGCTGTGCAG CAGAAGAGGC CAACTGCATT GGCAAAGATT 
CTCGGAGTTT ACAGGATAGG CTACAAGAAC TCCCAGAACA ACACTGAGAA GAAACTGGAC 
CTGCTCGTCA TGGAAAACCT GTTCTACGGA CGCAAGATGG CTCAGGTCTT TGACCTGAAA 
GGTTCCCTCA GAAACCGTCA TGTGAAGACA GACCTGGTAA AGGAAAGCTG CGAGGTGGTC 
CTGCTGGATG AGAATCTACT GAAGCTGATC CACGACAATC CGCTCTACAT TCGCTCTCAC 
TGCAAGTCCA TCTTGCGCGC CGCCATACAC AGCGACGCCT ACTTCCTGTC CAGCCACCTC 
ATCATTGACT ACTCCCTGCT GGTGGGGTGG GACGATGCCA CCGACCAGCT GGTGGTTGGG 
ATCATAGACT ATATTCGGAC GTTTACCTGG GACAAGAAAT TAGAGATGGT TGTCAAATCC 
ACTGGAATCC TGGGGGGTCA GGGAAAGATG CCCACCGTTG TTTCTCCTGA GCTGTACCGA 
GCTCGGTTCT GTGAGGCCAT GGACAAATAC TTCCTGATGG TACCCGACCA CTGGACGGGT 
CTTGGCATCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688496.1
CDS	132..6449
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQSMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832636.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832636.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGCATGATTC ACCAGGAACC TCAGAGCAGC 
GGCCTCAGTT CCAGACTGAC AGGACTTCAG GAGGATGTAG GAAAATCACC TGGTAGGAGG 
AGGTCTGCTA GCATCAGCAC ACTTCCACTT GACCGCTCTG GGTCATCAAT GGTACCTTCC 
TACGACAGCT CCGTCAGCCC CCCTACCAGC AGAGCCATGT CGGTGTCAAA GGCTGGGACA 
AAATTAGATC ACAGTGAGGA GGAACGTAAA ATACTACTGG ACTCCTCCCA GTTGAAGGAC 
TTATGGAGGA AAATCTGTCA TAATGGAACA GGGATGGAAT TCCAGGACCA CAGATACTGG 
CTGAGAACCT ACCCCAACTG CATTGTGGGA AAAGAGCTCG TCAACTGGCT GCTAAGAAAT 
GGCACCATTT CCAACAGGGT CCAAGCTATT GCCATAGGTC AGGCACTCGT GGATGGCCGC 
TGGCTCGACT GTGTCACCCA CCACGATCAG CTATTCAGAG ATGAGTATGC TCTCTATCGA 
CCACTTCAGA GTACAGAGTT CTCAGAAACT CCCTCCCCGG ACAGCGATAG TGTCAACTCA 
CTAGAGGGAC ATTCAGAGCC GTCATGGTTC AAGGACATCA AATTCGATGA CAGCGACACC 
GAGCAGCCCG CAGACGACAC AGAGTACACC ATGCCTAGTT CCGCTGGGCC CAGCAAGAGG 
ACATCTGTCA GCAGCGTCCA CTCAGCAGTA GACAGTGACT CAGCTGCCTC CATCAACCTC 
AACATGGAGC AAAACAATGT GAATTTTCGA ATCAAGAAAG ATTCCAAGTG TCCCCAGGCA 
TTCCCCGCCC TGGAGCACAA ATCTGAAATT ATGGCGTCTG AAGATGGAGG AGAGAACATC 
CTTATAAGTG ATGCCTTTAT CAAAGAGTCT CTCTTTAACC GTCGTGTGGA GGAGAAGGCT 
AAAGAAATGC TGTTTACTCC ACTGGGTTGG CACCACAGCT CTCTGGACCA GCTCAGAGAG 
GAGAATGGAG AAAAGAAGGC TATGGAGAGG CTGCTCTCGG CAAATCACAG CCACATGATG 
GCGCTGCTGC AGCAGTTGCT CTACAGCGAG TCTTTGTCTC TGTCCTGGCG GGATATTATC 
GTTCCTGTGG TTCGCCAGGT TGTCCAGACA GTTCGACCTG ATGTTCGCAA CAGTGATGAC 
GACATGGACA TCCGCCAACT GGTCCATATC AAAAAGATTC CTGGAGGAAA AAAGTTTGAC 
TCAACTGTAG TGAATGGCTT TGTGTGTACG AAGAGTATTG CCCACAAAAA GATGAACCCA 
TATATCAAGA ACCCCAAAAT TCTGCTGCTG AAATGCTCAA TAGAGTATCT GTACAGGGAA 
GAGACCAAAT TCACGTCCAT CGACCCCATC GTGCTGCAGG AACGAGAGTT TCTGAAAAAC 
TATGTGCAAC GTATCGTGGA TGTTCGGCCC ACTCTGGTTC TGGTGGAAAA AACGGTGTCT 
CGTATAGCTC AGGACATGCT GCTAGAACAC GGCATTACCC TGGTCATTAA TGTCAAACCG 
CAAGTATTGG ACAGGGTGAG TTGTGTGACC CAGGGGGACC TTGTCATGTC CATGGACCAG 
CTACTCACCA AACCTCGTCT GGGTACCTGC CACAAGTTTT ACATGCAGCC CTTCCCTTTG 
GCCAATAATG AGGTCAAGAC TCTGATGTTC TTCGAAGGAT GTCCTGCTCA TCTCGGGTGC 
TCCATAAATC TACGTGGTGC TTCAGACTAC GAGTTAGCCA GGGTGAAGGA GATCATGCGA 
TTAATGGTGT GTGTGGCCTA CCACTCTCAG CTGGAGATCT CCTTCCTCAT GGATGAGTTT 
GCCATGCCGC CCAGCTTGTC CCAGAGCACT TCATTTCCCT GTCTCCTCGA CGGAGGCTCT 
GCAGATGAGG AAGCAAGCAG GGAGAAACTA GAGAAGAGTT CTGAAGATGA AGACACTGTA 
GTGGAAGGAC AACCTGAGGA GGTGCCCTCT GCCAATGATG TAGACACTTC CCAGGACACA 
AATGATCCTA AATTATTCTC CTCTATGGTG GAAGAGACCA CTAATACTGA CACAGTCACG 
ACAAAGTCTT CCACTCCGTT CCAAAGCCCT CGTGCGCCAT GCTTGCCAGT TCCTCCACCA 
TTCATCATGG AAGAAACCCC AGAAATGGGC TCAGACAGCC TAACAGGGGC ATTTGAGGCA 
GGGGTGGTGG AATGCTGCCC AAAGGAGGAA TCAACCAACC TAGAAGATGG AGAAGATTCT 
GATATTCCTA CTCCTAAGTT GTTCCGAGAC CCCCTGCAAG ATGATACTGG GATGTTTGTG 
GCTGAAGAAG TTGCTTCTTC AGATGACCAT CTCAAGTCCA TCTCTGCTAT CTTCAAACAG 
GAACTTAAGG ACATCATCCT GTGCATTTCA CCTTTCATCA CATTTGAGAA ACCATTTCTT 
CTTACCCCTG CTGGGATGCA CTGCCCGAGC AGGGATTATT TTCCCGAACA GGTCTATCTG 
TCTCCTCTCC TCAACAAGGA CTTTAAAGAG TTGGATGGAC GCAGAAAGCG GCAGCTCCTT 
AAGGAGTCCA CTCCCTCCCT GGCAAACAGC CTAACGAATG GTGGCCCCCC GTCAAAGCAT 
GTCAGTGTCC TGCCATCCCA CAGTCTCACC AGCACCCGGA TCGTTGAACT AGTAAACAGG 
AGCCAGGGTC TGGCCAGAAT GGTTGCAGAC TACAGAGCAA AGGGTGGACG TATCCAGCAC 
AGCGAAGCTA CTGATCCATT TTGCATAAGC AGCTCTTCTG CTCCGGTCAG TGGCCAGAAT 
AGACCAGGTG ATGGTTCGGT CAGAGCACCA GTAAAGACTG ACAGCGAAGA GGAGAAGCCA 
ACTAAACCCA GTGACTGGAC CCCCAAGCTG GATTGTCTGA ACCCCACCAA CCACCAGAGA 
CTCTGTGTGC TTTTTAGCAG TTCATCAACT CAGTCCAGCA ATGCCCCCAA CCCCTGTGTC 
AACCCATGGA TTGTTACCAT GGAGTTTTAT GGCAAGAATG ACCTCTGCCT TGGTGTATTC 
TTGGAGCGAT ATTGCTTTAG GCCATCATAC CAGTGCCCCA GTATATTTTG TGAGACCCCC 
ATGGTGCACC ACATCCGGCG TTTTGTCCAT GGCAACGGCT GCGTGCAGAT AGTACTGAAG 
GAGCTGGACT CCCCTGTGCC GGGGTACCAG CACACCATTC TCAACTACTC CTGGTGCCGC 
ATCTGCAAAC AAGTGACACC AGTGGTTCCT CTATCCAATG ACTCATGGTC AATGTCTTTT 
GCAAAATATC TGGAGCTTCG CTTCTACGGC CACCAGTACA CCAGGAGGGC CAATGCAGAG 
CCCTGTGGCC ACTCCATCCA CAAAGACTAC CACCAGTACT TCTCATACAA CCAAATGGTG 
GCTTCCTTCA GTTACACCTC AGTGAGGCTG CTGGAGATTT GTCTTCCTCG TTTCAAAATC 
TTCATCAGGA ATCAGGGTCC TTCGATCAAC AACTTGGGGC AGGATCTGAA AGACTTCTCT 
CAAAAAGTAA ATCAGGTTTA CGTGGCCATA GATGATCGTC TCACATCTCT AAAGACGGAC 
ACCTTCAGTA AAACACGCGA GGAGAAGATG GAGGACCTCT TTGCACAGAA AGATATGGAA 
GAGGCGGAAC TGCGCAGCTG GATTGAAAAG TTTCAAGCAC GATTGCAGGC TTGTGGTCAC 
GTTTCTCCTC AGCAGCTGCA GACGACTCTG GAATCACTGG TGATGAAGAA ACAGAGTCTG 
TGTGAGATGT TGCAGTACTG GAACAGCAGG CTGCAGGAAT TATTCCAACA GGAGAAAGGC 
AGGAAACGTC TGTCAGTGCC CCCAAGCCCC GGCAGGCACA GACAGACTGC CCCTGATGAT 
GGCAAGAACG CTGTGGAGTC ATCCCCACGT AACCCTTCAC CTGTGGTACA GAATGGTGAA 
AAGGAGGACC GTCACCTCAC CACACTGCCT TCAACCTCCT CTCTGTTGCC CTCGCCTGGA 
GACCTGGGAG CTGAACCAGC CACATCTGGT CCTTCCCTGA GTGAGCAGGA CTCTGTCAGC 
ATCCCAGAAG ATGTATTTGA TGGACACTTG CTGGGCTCCA ATGACAGTCA GGTGAAGGAG 
AAATCCACCA TGAAAGCCAT CCTTGCCAAC TTCCTGCCCG GCAACAGTTA CAACCCCATT 
CCATTCCCAT TTGACCCAGA CAAACACTAC CTGATGGATG AGTATGAGCG AGTGCCAATT 
GCAGTGTGTG AAAGAGAACC AAGCTCCATC ATTGCATTTG CTCTGAGTTG TAAGAAGTAT 
AAAACAGCAC TGGAAGATCT GTCCAAGGTG TCAAACATGG GTGGAGAAGA CTCTTCACAA 
GCTGCCAGTA GTGGAGAGAG TCGGGTGAGG GGCAGCCCCG CCAGGCCCAG TGAGTCAGCC 
TCGTCCCAAC AGAGCCGCAG CAGCAGTGAA GCAGATCCCC TCAGTAGGCG TCCAGAAGAC 
GCAGACCTAA CAGACAAACA GAAGAAGCAG GCCCTGAATC CCCACATTGA GCTACAGTTC 
TCTGATGCAA GCGCAAAGTT TTACTGTCGG ATCTACTACG CCGAGGAGTT TCATAAGATG 
CGTGAGGAGA TCATGGAGAG CTCCGAAGAG GACTTTGTGC GTTCCCTGTC GCACTGTGTC 
AACTGGCAGG CGCGCGGTGG AAAGTCTGGG GCCGTTTTCT ACGCAACAGA AGACGACCGT 
TTCATCCTAA AACAGATGCC CAGGCTGGAG GTGCAGTCCT TCTTGGACTT TGCTCCGCAC 
TACTTCACCT ACATCACCGG AGCTGTGCAG CAGAAGAGGC CAACTGCATT GGCAAAGATT 
CTCGGAGTTT ACAGGATAGG CTACAAGAAC TCCCAGAACA ACACTGAGAA GAAACTGGAC 
CTGCTCGTCA TGGAAAACCT GTTCTACGGA CGCAAGATGG CTCAGGTCTT TGACCTGAAA 
GGTTCCCTCA GAAACCGTCA TGTGAAGACA GACCTGGTAA AGGAAAGCTG CGAGGTGGTC 
CTGCTGGATG AGAATCTACT GAAGCTGATC CACGACAATC CGCTCTACAT TCGCTCTCAC 
TGCAAGTCCA TCTTGCGCGC CGCCATACAC AGCGACGCCT ACTTCCTGTC CAGCCACCTC 
ATCATTGACT ACTCCCTGCT GGTGGGGTGG GACGATGCCA CCGACCAGCT GGTGGTTGGG 
ATCATAGACT ATATTCGGAC GTTTACCTGG GACAAGAAAT TAGAGATGGT TGTCAAATCC 
ACTGGAATCC TGGGGGGTCA GGGAAAGATG CCCACCGTTG TTTCTCCTGA GCTGTACCGA 
GCTCGGTTCT GTGAGGCCAT GGACAAATAC TTCCTGATGG TACCCGACCA CTGGACGGGT 
CTTGGCATCA ACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832639.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688499.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X14, mRNA.

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CloneID	OTo40932
Clone ID Related Accession (Same CDS sequence)	XM_029832639.1
Accession Version	XM_029832639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGCATGATTC ACCAGGAACC TCAGAGCAGC 
GGCCTCAGTT CCAGACTGAC AGGACTTCAG GAGGATGTAG GAAAATCACC TGGTAGGAGG 
AGGTCTGCTA GCATCAGCAC ACTTCCACTT GACCGCTCTG GGTCATCAAT GGTACCTTCC 
TACGACAGCT CCGTCAGCCC CCCTACCAGC AGAGCCATGT CGGTGTCAAA GGCTGGGACA 
AAATTAGATC ACAGTGAGGA GGAACGTAAA ATACTACTGG ACTCCTCCCA GTTGAAGGAC 
TTATGGAGGA AAATCTGTCA TAATGGAACA GGGATGGAAT TCCAGGACCA CAGATACTGG 
CTGAGAACCT ACCCCAACTG CATTGTGGGA AAAGAGCTCG TCAACTGGCT GCTAAGAAAT 
GGCACCATTT CCAACAGGGT CCAAGCTATT GCCATAGGTC AGGCACTCGT GGATGGCCGC 
TGGCTCGACT GTGTCACCCA CCACGATCAG CTATTCAGAG ATGAGTATGC TCTCTATCGA 
CCACTTCAGA GTACAGAGTT CTCAGAAACT CCCTCCCCGG ACAGCGATAG TGTCAACTCA 
CTAGAGGGAC ATTCAGAGCC GTCATGGTTC AAGGACATCA AATTCGATGA CAGCGACACC 
GAGCAGCCCG CAGACGACAC AGAGTACACC ATGCCTAGTT CCGCTGGGCC CAGCAAGAGG 
ACATCTGTCA GCAGCGTCCA CTCAGCAGTA GACAGTGACT CAGCTGCCTC CATCAACCTC 
AACATGGAGC AAAACAATGT GAATTTTCGA ATCAAGAAAG ATTCCAAGTG TCCCCAGGCA 
TTCCCCGCCC TGGAGCACAA ATCTGAAATT ATGGCGTCTG AAGATGGAGG AGAGAACATC 
CTTATAAGTG ATGCCTTTAT CAAAGAGTCT CTCTTTAACC GTCGTGTGGA GGAGAAGGCT 
AAAGAAATGC TGTTTACTCC ACTGGGTTGG CACCACAGCT CTCTGGACCA GCTCAGAGAG 
GAGAATGGAG AAAAGAAGGC TATGGAGAGG CTGCTCTCGG CAAATCACAG CCACATGATG 
GCGCTGCTGC AGCAGTTGCT CTACAGCGAG TCTTTGTCTC TGTCCTGGCG GGATATTATC 
GTTCCTGTGG TTCGCCAGGT TGTCCAGACA GTTCGACCTG ATGTTCGCAA CAGTGATGAC 
GACATGGACA TCCGCCAACT GGTCCATATC AAAAAGATTC CTGGAGGAAA AAAGTTTGAC 
TCAACTGTAG TGAATGGCTT TGTGTGTACG AAGAGTATTG CCCACAAAAA GATGAACCCA 
TATATCAAGA ACCCCAAAAT TCTGCTGCTG AAATGCTCAA TAGAGTATCT GTACAGGGAA 
GAGACCAAAT TCACGTCCAT CGACCCCATC GTGCTGCAGG AACGAGAGTT TCTGAAAAAC 
TATGTGCAAC GTATCGTGGA TGTTCGGCCC ACTCTGGTTC TGGTGGAAAA AACGGTGTCT 
CGTATAGCTC AGGACATGCT GCTAGAACAC GGCATTACCC TGGTCATTAA TGTCAAACCG 
CAAGTATTGG ACAGGGTGAG TTGTGTGACC CAGGGGGACC TTGTCATGTC CATGGACCAG 
CTACTCACCA AACCTCGTCT GGGTACCTGC CACAAGTTTT ACATGCAGCC CTTCCCTTTG 
GCCAATAATG AGGTCAAGAC TCTGATGTTC TTCGAAGGAT GTCCTGCTCA TCTCGGGTGC 
TCCATAAATC TACGTGGTGC TTCAGACTAC GAGTTAGCCA GGGTGAAGGA GATCATGCGA 
TTAATGGTGT GTGTGGCCTA CCACTCTCAG CTGGAGATCT CCTTCCTCAT GGATGAGTTT 
GCCATGCCGC CCAGCTTGTC CCAGAGCACT TCATTTCCCT GTCTCCTCGA CGGAGGCTCT 
GCAGATGAGG AAGCAAGCAG GGAGAAACTA GAGAAGAGTT CTGAAGATGA AGACACTGTA 
GTGGAAGGAC AACCTGAGGA GGTGCCCTCT GCCAATGATG TAGACACTTC CCAGGACACA 
AATGATCCTA AATTATTCTC CTCTATGGTG GAAGAGACCA CTAATACTGA CACAGTCACG 
ACAAAGTCTT CCACTCCGTT CCAAAGCCCT CGTGCGCCAT GCTTGCCAGT TCCTCCACCA 
TTCATCATGG AAGAAACCCC AGAAATGGGC TCAGACAGCC TAACAGGGGC ATTTGAGGCA 
GGGGTGGTGG AATGCTGCCC AAAGGAGGAA TCAACCAACC TAGAAGATGG AGAAGATTCT 
GATATTCCTA CTCCTAAGTT GTTCCGAGAC CCCCTGCAAG ATGATACTGG GATGTTTGTG 
GCTGAAGAAG TTGCTTCTTC AGATGACCAT CTCAAGTCCA TCTCTGCTAT CTTCAAACAG 
GAACTTAAGG ACATCATCCT GTGCATTTCA CCTTTCATCA CATTTGAGAA ACCATTTCTT 
CTTACCCCTG CTGGGATGCA CTGCCCGAGC AGGGATTATT TTCCCGAACA GGTCTATCTG 
TCTCCTCTCC TCAACAAGGA CTTTAAAGAG TTGGATGGAC GCAGAAAGCG GCAGCTCCTT 
AAGGAGTCCA CTCCCTCCCT GGCAAACAGC CTAACGAATG GTGGCCCCCC GTCAAAGCAT 
GTCAGTGTCC TGCCATCCCA CAGTCTCACC AGCACCCGGA TCGTTGAACT AGTAAACAGG 
AGCCAGGGTC TGGCCAGAAT GGTTGCAGAC TACAGAGCAA AGGGTGGACG TATCCAGCAC 
AGCGAAGCTA CTGATCCATT TTGCATAAGC AGCTCTTCTG CTCCGGTCAG TGGCCAGAAT 
AGACCAGGTG ATGGTTCGGT CAGAGCACCA GTAAAGACTG ACAGCGAAGA GGAGAAGCCA 
ACTAAACCCA GTGACTGGAC CCCCAAGCTG GATTGTCTGA ACCCCACCAA CCACCAGAGA 
CTCTGTGTGC TTTTTAGCAG TTCATCAACT CAGTCCAGCA ATGCCCCCAA CCCCTGTGTC 
AACCCATGGA TTGTTACCAT GGAGTTTTAT GGCAAGAATG ACCTCTGCCT TGGTGTATTC 
TTGGAGCGAT ATTGCTTTAG GCCATCATAC CAGTGCCCCA GTATATTTTG TGAGACCCCC 
ATGGTGCACC ACATCCGGCG TTTTGTCCAT GGCAACGGCT GCGTGCAGAT AGTACTGAAG 
GAGCTGGACT CCCCTGTGCC GGGGTACCAG CACACCATTC TCAACTACTC CTGGTGCCGC 
ATCTGCAAAC AAGTGACACC AGTGGTTCCT CTATCCAATG ACTCATGGTC AATGTCTTTT 
GCAAAATATC TGGAGCTTCG CTTCTACGGC CACCAGTACA CCAGGAGGGC CAATGCAGAG 
CCCTGTGGCC ACTCCATCCA CAAAGACTAC CACCAGTACT TCTCATACAA CCAAATGGTG 
GCTTCCTTCA GTTACACCTC AGTGAGGCTG CTGGAGATTT GTCTTCCTCG TTTCAAAATC 
TTCATCAGGA ATCAGGGTCC TTCGATCAAC AACTTGGGGC AGGATCTGAA AGACTTCTCT 
CAAAAAGTAA ATCAGGTTTA CGTGGCCATA GATGATCGTC TCACATCTCT AAAGACGGAC 
ACCTTCAGTA AAACACGCGA GGAGAAGATG GAGGACCTCT TTGCACAGAA AGATATGGAA 
GAGGCGGAAC TGCGCAGCTG GATTGAAAAG TTTCAAGCAC GATTGCAGGC TTGTGGTCAC 
GTTTCTCCTC AGCAGCTGCA GACGACTCTG GAATCACTGG TGATGAAGAA ACAGAGTCTG 
TGTGAGATGT TGCAGTACTG GAACAGCAGG CTGCAGGAAT TATTCCAACA GGAGAAAGGC 
AGGAAACGTC TGTCAGTGCC CCCAAGCCCC GGCAGGCACA GACAGACTGC CCCTGATGAT 
GGCAAGAACG CTGTGGAGTC ATCCCCACGT AACCCTTCAC CTGTGGTACA GAATGGTGAA 
AAGGAGGACC GTCACCTCAC CACACTGCCT TCAACCTCCT CTCTGTTGCC CTCGCCTGGA 
GACCTGGGAG CTGAACCAGC CACATCTGGT CCTTCCCTGA GTGAGCAGGA CTCTGTCAGC 
ATCCCAGAAG ATGTATTTGA TGGACACTTG CTGGGCTCCA ATGACAGTCA GGTGAAGGAG 
AAATCCACCA TGAAAGCCAT CCTTGCCAAC TTCCTGCCCG GCAACAGTTA CAACCCCATT 
CCATTCCCAT TTGACCCAGA CAAACACTAC CTGATGGATG AGTATGAGCG AGTGCCAATT 
GCAGTGTGTG AAAGAGAACC AAGCTCCATC ATTGCATTTG CTCTGAGTTG TAAGAAGTAT 
AAAACAGCAC TGGAAGATCT GTCCAAGGTG TCAAACATGG GTGGAGAAGA CTCTTCACAA 
GCTGCCAGTA GTGGAGAGAG TCGGGTGAGG GGCAGCCCCG CCAGGCCCAG TGAGTCAGCC 
TCGTCCCAAC AGAGCCGCAG CAGCAGTGAA GCAGATCCCC TCAAAGACGC AGACCTAACA 
GACAAACAGA AGAAGCAGGC CCTGAATCCC CACATTGAGC TACAGTTCTC TGATGCAAGC 
GCAAAGTTTT ACTGTCGGAT CTACTACGCC GAGGAGTTTC ATAAGATGCG TGAGGAGATC 
ATGGAGAGCT CCGAAGAGGA CTTTGTGCGT TCCCTGTCGC ACTGTGTCAA CTGGCAGGCG 
CGCGGTGGAA AGTCTGGGGC CGTTTTCTAC GCAACAGAAG ACGACCGTTT CATCCTAAAA 
CAGATGCCCA GGCTGGAGGT GCAGTCCTTC TTGGACTTTG CTCCGCACTA CTTCACCTAC 
ATCACCGGAG CTGTGCAGCA GAAGAGGCCA ACTGCATTGG CAAAGATTCT CGGAGTTTAC 
AGGATAGGCT ACAAGAACTC CCAGAACAAC ACTGAGAAGA AACTGGACCT GCTCGTCATG 
GAAAACCTGT TCTACGGACG CAAGATGGCT CAGGTCTTTG ACCTGAAAGG TTCCCTCAGA 
AACCGTCATG TGAAGACAGA CCTGGTAAAG GAAAGCTGCG AGGTGGTCCT GCTGGATGAG 
AATCTACTGA AGCTGATCCA CGACAATCCG CTCTACATTC GCTCTCACTG CAAGTCCATC 
TTGCGCGCCG CCATACACAG CGACGCCTAC TTCCTGTCCA GCCACCTCAT CATTGACTAC 
TCCCTGCTGG TGGGGTGGGA CGATGCCACC GACCAGCTGG TGGTTGGGAT CATAGACTAT 
ATTCGGACGT TTACCTGGGA CAAGAAATTA GAGATGGTTG TCAAATCCAC TGGAATCCTG 
GGGGGTCAGG GAAAGATGCC CACCGTTGTT TCTCCTGAGC TGTACCGAGC TCGGTTCTGT 
GAGGCCATGG ACAAATACTT CCTGATGGTA CCCGACCACT GGACGGGTCT TGGCATCAAC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688499.1
CDS	129..6434
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQSMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832639.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832639.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGTCACA GGCAGTGACC ACAGTCTTGT GAAGTCAGGT 
GATAGAAAGG GCCTGTCTGG ATTGATGCCA GGAACCAAGG AGGGACGTTC TCTTTCAACA 
GTGTCAGAGA AATCAGACAC TCCATCCCCT TCCCCTCTAT CAGAGAGGAG GAGCTGGTCC 
TCCAGCCCAC CCCATTCCCT CTACACTGCC AGGATTCCCC GAAAACAACC AGATCACCTT 
CAAAGGACCT CTGCTGCTTC AGATGGCAGC AGGAAATCAG AGACTCCTTT AAGCAATCAC 
GACCCTCGAA CTGCTGTGCA ACTTCGCACT GCATTGAAGA GGCTGAAGGA AATAATGGAG 
GGAAAGAGCC AGGACAGTGA CTTGAAGCAG TACTGGATGC CGGACAGCCA GTGTAAAGAA 
TGTTATGACT GCAATGAAAA GTTCACAACC TTCCGACGCC GCCACCATTG CCGACTGTGT 
GGTCAGATTT TCTGCAGTCG CTGCTGCAAC CAGGAAATCC CTGGAAAGTT CATGGGTTAC 
ACAGGAGATT TGCGGGCCTG TACGTACTGC CGTAAGTTGG CACTGAGCTA CGCCCACTCG 
GCTGATTCGG GCTCCATCGG CGAAGACTTG AACACCCTGA CTGACTCTCC GAGCTCTGTG 
TGTGTGCTGG AGCCCAGTGA GCCACGGACG CCTGTGGGGG GGCGCAAGGC CAGCAGGAAC 
ATCTTCCTAG AGGAAGACTT AACATGGCAG AGCATGATTC ACCAGGAACC TCAGAGCAGC 
GGCCTCAGTT CCAGACTGAC AGGACTTCAG GAGGATGTAG GAAAATCACC TGGTAGGAGG 
AGGTCTGCTA GCATCAGCAC ACTTCCACTT GACCGCTCTG GGTCATCAAT GGTACCTTCC 
TACGACAGCT CCGTCAGCCC CCCTACCAGC AGAGCCATGT CGGTGTCAAA GGCTGGGACA 
AAATTAGATC ACAGTGAGGA GGAACGTAAA ATACTACTGG ACTCCTCCCA GTTGAAGGAC 
TTATGGAGGA AAATCTGTCA TAATGGAACA GGGATGGAAT TCCAGGACCA CAGATACTGG 
CTGAGAACCT ACCCCAACTG CATTGTGGGA AAAGAGCTCG TCAACTGGCT GCTAAGAAAT 
GGCACCATTT CCAACAGGGT CCAAGCTATT GCCATAGGTC AGGCACTCGT GGATGGCCGC 
TGGCTCGACT GTGTCACCCA CCACGATCAG CTATTCAGAG ATGAGTATGC TCTCTATCGA 
CCACTTCAGA GTACAGAGTT CTCAGAAACT CCCTCCCCGG ACAGCGATAG TGTCAACTCA 
CTAGAGGGAC ATTCAGAGCC GTCATGGTTC AAGGACATCA AATTCGATGA CAGCGACACC 
GAGCAGCCCG CAGACGACAC AGAGTACACC ATGCCTAGTT CCGCTGGGCC CAGCAAGAGG 
ACATCTGTCA GCAGCGTCCA CTCAGCAGTA GACAGTGACT CAGCTGCCTC CATCAACCTC 
AACATGGAGC AAAACAATGT GAATTTTCGA ATCAAGAAAG ATTCCAAGTG TCCCCAGGCA 
TTCCCCGCCC TGGAGCACAA ATCTGAAATT ATGGCGTCTG AAGATGGAGG AGAGAACATC 
CTTATAAGTG ATGCCTTTAT CAAAGAGTCT CTCTTTAACC GTCGTGTGGA GGAGAAGGCT 
AAAGAAATGC TGTTTACTCC ACTGGGTTGG CACCACAGCT CTCTGGACCA GCTCAGAGAG 
GAGAATGGAG AAAAGAAGGC TATGGAGAGG CTGCTCTCGG CAAATCACAG CCACATGATG 
GCGCTGCTGC AGCAGTTGCT CTACAGCGAG TCTTTGTCTC TGTCCTGGCG GGATATTATC 
GTTCCTGTGG TTCGCCAGGT TGTCCAGACA GTTCGACCTG ATGTTCGCAA CAGTGATGAC 
GACATGGACA TCCGCCAACT GGTCCATATC AAAAAGATTC CTGGAGGAAA AAAGTTTGAC 
TCAACTGTAG TGAATGGCTT TGTGTGTACG AAGAGTATTG CCCACAAAAA GATGAACCCA 
TATATCAAGA ACCCCAAAAT TCTGCTGCTG AAATGCTCAA TAGAGTATCT GTACAGGGAA 
GAGACCAAAT TCACGTCCAT CGACCCCATC GTGCTGCAGG AACGAGAGTT TCTGAAAAAC 
TATGTGCAAC GTATCGTGGA TGTTCGGCCC ACTCTGGTTC TGGTGGAAAA AACGGTGTCT 
CGTATAGCTC AGGACATGCT GCTAGAACAC GGCATTACCC TGGTCATTAA TGTCAAACCG 
CAAGTATTGG ACAGGGTGAG TTGTGTGACC CAGGGGGACC TTGTCATGTC CATGGACCAG 
CTACTCACCA AACCTCGTCT GGGTACCTGC CACAAGTTTT ACATGCAGCC CTTCCCTTTG 
GCCAATAATG AGGTCAAGAC TCTGATGTTC TTCGAAGGAT GTCCTGCTCA TCTCGGGTGC 
TCCATAAATC TACGTGGTGC TTCAGACTAC GAGTTAGCCA GGGTGAAGGA GATCATGCGA 
TTAATGGTGT GTGTGGCCTA CCACTCTCAG CTGGAGATCT CCTTCCTCAT GGATGAGTTT 
GCCATGCCGC CCAGCTTGTC CCAGAGCACT TCATTTCCCT GTCTCCTCGA CGGAGGCTCT 
GCAGATGAGG AAGCAAGCAG GGAGAAACTA GAGAAGAGTT CTGAAGATGA AGACACTGTA 
GTGGAAGGAC AACCTGAGGA GGTGCCCTCT GCCAATGATG TAGACACTTC CCAGGACACA 
AATGATCCTA AATTATTCTC CTCTATGGTG GAAGAGACCA CTAATACTGA CACAGTCACG 
ACAAAGTCTT CCACTCCGTT CCAAAGCCCT CGTGCGCCAT GCTTGCCAGT TCCTCCACCA 
TTCATCATGG AAGAAACCCC AGAAATGGGC TCAGACAGCC TAACAGGGGC ATTTGAGGCA 
GGGGTGGTGG AATGCTGCCC AAAGGAGGAA TCAACCAACC TAGAAGATGG AGAAGATTCT 
GATATTCCTA CTCCTAAGTT GTTCCGAGAC CCCCTGCAAG ATGATACTGG GATGTTTGTG 
GCTGAAGAAG TTGCTTCTTC AGATGACCAT CTCAAGTCCA TCTCTGCTAT CTTCAAACAG 
GAACTTAAGG ACATCATCCT GTGCATTTCA CCTTTCATCA CATTTGAGAA ACCATTTCTT 
CTTACCCCTG CTGGGATGCA CTGCCCGAGC AGGGATTATT TTCCCGAACA GGTCTATCTG 
TCTCCTCTCC TCAACAAGGA CTTTAAAGAG TTGGATGGAC GCAGAAAGCG GCAGCTCCTT 
AAGGAGTCCA CTCCCTCCCT GGCAAACAGC CTAACGAATG GTGGCCCCCC GTCAAAGCAT 
GTCAGTGTCC TGCCATCCCA CAGTCTCACC AGCACCCGGA TCGTTGAACT AGTAAACAGG 
AGCCAGGGTC TGGCCAGAAT GGTTGCAGAC TACAGAGCAA AGGGTGGACG TATCCAGCAC 
AGCGAAGCTA CTGATCCATT TTGCATAAGC AGCTCTTCTG CTCCGGTCAG TGGCCAGAAT 
AGACCAGGTG ATGGTTCGGT CAGAGCACCA GTAAAGACTG ACAGCGAAGA GGAGAAGCCA 
ACTAAACCCA GTGACTGGAC CCCCAAGCTG GATTGTCTGA ACCCCACCAA CCACCAGAGA 
CTCTGTGTGC TTTTTAGCAG TTCATCAACT CAGTCCAGCA ATGCCCCCAA CCCCTGTGTC 
AACCCATGGA TTGTTACCAT GGAGTTTTAT GGCAAGAATG ACCTCTGCCT TGGTGTATTC 
TTGGAGCGAT ATTGCTTTAG GCCATCATAC CAGTGCCCCA GTATATTTTG TGAGACCCCC 
ATGGTGCACC ACATCCGGCG TTTTGTCCAT GGCAACGGCT GCGTGCAGAT AGTACTGAAG 
GAGCTGGACT CCCCTGTGCC GGGGTACCAG CACACCATTC TCAACTACTC CTGGTGCCGC 
ATCTGCAAAC AAGTGACACC AGTGGTTCCT CTATCCAATG ACTCATGGTC AATGTCTTTT 
GCAAAATATC TGGAGCTTCG CTTCTACGGC CACCAGTACA CCAGGAGGGC CAATGCAGAG 
CCCTGTGGCC ACTCCATCCA CAAAGACTAC CACCAGTACT TCTCATACAA CCAAATGGTG 
GCTTCCTTCA GTTACACCTC AGTGAGGCTG CTGGAGATTT GTCTTCCTCG TTTCAAAATC 
TTCATCAGGA ATCAGGGTCC TTCGATCAAC AACTTGGGGC AGGATCTGAA AGACTTCTCT 
CAAAAAGTAA ATCAGGTTTA CGTGGCCATA GATGATCGTC TCACATCTCT AAAGACGGAC 
ACCTTCAGTA AAACACGCGA GGAGAAGATG GAGGACCTCT TTGCACAGAA AGATATGGAA 
GAGGCGGAAC TGCGCAGCTG GATTGAAAAG TTTCAAGCAC GATTGCAGGC TTGTGGTCAC 
GTTTCTCCTC AGCAGCTGCA GACGACTCTG GAATCACTGG TGATGAAGAA ACAGAGTCTG 
TGTGAGATGT TGCAGTACTG GAACAGCAGG CTGCAGGAAT TATTCCAACA GGAGAAAGGC 
AGGAAACGTC TGTCAGTGCC CCCAAGCCCC GGCAGGCACA GACAGACTGC CCCTGATGAT 
GGCAAGAACG CTGTGGAGTC ATCCCCACGT AACCCTTCAC CTGTGGTACA GAATGGTGAA 
AAGGAGGACC GTCACCTCAC CACACTGCCT TCAACCTCCT CTCTGTTGCC CTCGCCTGGA 
GACCTGGGAG CTGAACCAGC CACATCTGGT CCTTCCCTGA GTGAGCAGGA CTCTGTCAGC 
ATCCCAGAAG ATGTATTTGA TGGACACTTG CTGGGCTCCA ATGACAGTCA GGTGAAGGAG 
AAATCCACCA TGAAAGCCAT CCTTGCCAAC TTCCTGCCCG GCAACAGTTA CAACCCCATT 
CCATTCCCAT TTGACCCAGA CAAACACTAC CTGATGGATG AGTATGAGCG AGTGCCAATT 
GCAGTGTGTG AAAGAGAACC AAGCTCCATC ATTGCATTTG CTCTGAGTTG TAAGAAGTAT 
AAAACAGCAC TGGAAGATCT GTCCAAGGTG TCAAACATGG GTGGAGAAGA CTCTTCACAA 
GCTGCCAGTA GTGGAGAGAG TCGGGTGAGG GGCAGCCCCG CCAGGCCCAG TGAGTCAGCC 
TCGTCCCAAC AGAGCCGCAG CAGCAGTGAA GCAGATCCCC TCAAAGACGC AGACCTAACA 
GACAAACAGA AGAAGCAGGC CCTGAATCCC CACATTGAGC TACAGTTCTC TGATGCAAGC 
GCAAAGTTTT ACTGTCGGAT CTACTACGCC GAGGAGTTTC ATAAGATGCG TGAGGAGATC 
ATGGAGAGCT CCGAAGAGGA CTTTGTGCGT TCCCTGTCGC ACTGTGTCAA CTGGCAGGCG 
CGCGGTGGAA AGTCTGGGGC CGTTTTCTAC GCAACAGAAG ACGACCGTTT CATCCTAAAA 
CAGATGCCCA GGCTGGAGGT GCAGTCCTTC TTGGACTTTG CTCCGCACTA CTTCACCTAC 
ATCACCGGAG CTGTGCAGCA GAAGAGGCCA ACTGCATTGG CAAAGATTCT CGGAGTTTAC 
AGGATAGGCT ACAAGAACTC CCAGAACAAC ACTGAGAAGA AACTGGACCT GCTCGTCATG 
GAAAACCTGT TCTACGGACG CAAGATGGCT CAGGTCTTTG ACCTGAAAGG TTCCCTCAGA 
AACCGTCATG TGAAGACAGA CCTGGTAAAG GAAAGCTGCG AGGTGGTCCT GCTGGATGAG 
AATCTACTGA AGCTGATCCA CGACAATCCG CTCTACATTC GCTCTCACTG CAAGTCCATC 
TTGCGCGCCG CCATACACAG CGACGCCTAC TTCCTGTCCA GCCACCTCAT CATTGACTAC 
TCCCTGCTGG TGGGGTGGGA CGATGCCACC GACCAGCTGG TGGTTGGGAT CATAGACTAT 
ATTCGGACGT TTACCTGGGA CAAGAAATTA GAGATGGTTG TCAAATCCAC TGGAATCCTG 
GGGGGTCAGG GAAAGATGCC CACCGTTGTT TCTCCTGAGC TGTACCGAGC TCGGTTCTGT 
GAGGCCATGG ACAAATACTT CCTGATGGTA CCCGACCACT GGACGGGTCT TGGCATCAAC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832642.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688502.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X15, mRNA.

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CloneID	OTo40933
Clone ID Related Accession (Same CDS sequence)	XM_029832642.1
Accession Version	XM_029832642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGAGGAG GGACGTTCTC TTTCAACAGT GTCAGAGAAA 
TCAGACACTC CATCCCCTTC CCCTCTATCA GAGAGGAGGA GCTGGTCCTC CAGCCCACCC 
CATTCCCTCT ACACTGCCAG GATTCCCCGA AAACAACCAG ATCACCTTCA AAGGACCTCT 
GCTGCTTCAG ATGGCAGCAG GAAATCAGAG ACTCCTTTAA GCAATCACGA CCCTCGAACT 
GCTGTGCAAC TTCGCACTGC ATTGAAGAGG CTGAAGGAAA TAATGGAGGG AAAGAGCCAG 
GACAGTGACT TGAAGCAGTA CTGGATGCCG GACAGCCAGT GTAAAGAATG TTATGACTGC 
AATGAAAAGT TCACAACCTT CCGACGCCGC CACCATTGCC GACTGTGTGG TCAGATTTTC 
TGCAGTCGCT GCTGCAACCA GGAAATCCCT GGAAAGTTCA TGGGTTACAC AGGAGATTTG 
CGGGCCTGTA CGTACTGCCG TAAGTTGGCA CTGAGCTACG CCCACTCGGC TGATTCGGGC 
TCCATCGGCG AAGACTTGAA CACCCTGACT GACTCTCCGA GCTCTGTGTG TGTGCTGGAG 
CCCAGTGAGC CACGGACGCC TGTGGGGGGG CGCAAGGCCA GCAGGAACAT CTTCCTAGAG 
GAAGACTTAA CATGGCAGAG AAAAACACCT ATTGGGATGA GAAAGAACAT GATTCACCAG 
GAACCTCAGA GCAGCGGCCT CAGTTCCAGA CTGACAGGAC TTCAGGAGGA TGTAGGAAAA 
TCACCTGGTA GGAGGAGGTC TGCTAGCATC AGCACACTTC CACTTGACCG CTCTGGGTCA 
TCAATGGTAC CTTCCTACGA CAGCTCCGTC AGCCCCCCTA CCAGCAGAGC CATGTCGGTG 
TCAAAGGCTG GGACAAAATT AGATCACAGT GAGGAGGAAC GTAAAATACT ACTGGACTCC 
TCCCAGTTGA AGGACTTATG GAGGAAAATC TGTCATAATG GAACAGGGAT GGAATTCCAG 
GACCACAGAT ACTGGCTGAG AACCTACCCC AACTGCATTG TGGGAAAAGA GCTCGTCAAC 
TGGCTGCTAA GAAATGGCAC CATTTCCAAC AGGGTCCAAG CTATTGCCAT AGGTCAGGCA 
CTCGTGGATG GCCGCTGGCT CGACTGTGTC ACCCACCACG ATCAGCTATT CAGAGATGAG 
TATGCTCTCT ATCGACCACT TCAGAGTACA GAGTTCTCAG AAACTCCCTC CCCGGACAGC 
GATAGTGTCA ACTCACTAGA GGGACATTCA GAGCCGTCAT GGTTCAAGGA CATCAAATTC 
GATGACAGCG ACACCGAGCA GCCCGCAGAC GACACAGAGT ACACCATGCC TAGTTCCGCT 
GGGCCCAGCA AGAGGACATC TGTCAGCAGC GTCCACTCAG CAGTAGACAG TGACTCAGCT 
GCCTCCATCA ACCTCAACAT GGAGCAAAAC AATGTGAATT TTCGAATCAA GAAAGATTCC 
AAGTGTCCCC AGGCATTCCC CGCCCTGGAG CACAAATCTG AAATTATGGC GTCTGAAGAT 
GGAGGAGAGA ACATCCTTAT AAGTGATGCC TTTATCAAAG AGTCTCTCTT TAACCGTCGT 
GTGGAGGAGA AGGCTAAAGA AATGCTGTTT ACTCCACTGG GTTGGCACCA CAGCTCTCTG 
GACCAGCTCA GAGAGGAGAA TGGAGAAAAG AAGGCTATGG AGAGGCTGCT CTCGGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAG TTGCTCTACA GCGAGTCTTT GTCTCTGTCC 
TGGCGGGATA TTATCGTTCC TGTGGTTCGC CAGGTTGTCC AGACAGTTCG ACCTGATGTT 
CGCAACAGTG ATGACGACAT GGACATCCGC CAACTGGTCC ATATCAAAAA GATTCCTGGA 
GGAAAAAAGT TTGACTCAAC TGTAGTGAAT GGCTTTGTGT GTACGAAGAG TATTGCCCAC 
AAAAAGATGA ACCCATATAT CAAGAACCCC AAAATTCTGC TGCTGAAATG CTCAATAGAG 
TATCTGTACA GGGAAGAGAC CAAATTCACG TCCATCGACC CCATCGTGCT GCAGGAACGA 
GAGTTTCTGA AAAACTATGT GCAACGTATC GTGGATGTTC GGCCCACTCT GGTTCTGGTG 
GAAAAAACGG TGTCTCGTAT AGCTCAGGAC ATGCTGCTAG AACACGGCAT TACCCTGGTC 
ATTAATGTCA AACCGCAAGT ATTGGACAGG GTGAGTTGTG TGACCCAGGG GGACCTTGTC 
ATGTCCATGG ACCAGCTACT CACCAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACATG 
CAGCCCTTCC CTTTGGCCAA TAATGAGGTC AAGACTCTGA TGTTCTTCGA AGGATGTCCT 
GCTCATCTCG GGTGCTCCAT AAATCTACGT GGTGCTTCAG ACTACGAGTT AGCCAGGGTG 
AAGGAGATCA TGCGATTAAT GGTGTGTGTG GCCTACCACT CTCAGCTGGA GATCTCCTTC 
CTCATGGATG AGTTTGCCAT GCCGCCCAGC TTGTCCCAGA GCACTTCATT TCCCTGTCTC 
CTCGACGGAG GCTCTGCAGA TGAGGAAGCA AGCAGGGAGA AACTAGAGAA GAGTTCTGAA 
GATGAAGACA CTGTAGTGGA AGGACAACCT GAGGAGGTGC CCTCTGCCAA TGATGTAGAC 
ACTTCCCAGG ACACAAATGA TCCTAAATTA TTCTCCTCTA TGGTGGAAGA GACCACTAAT 
ACTGACACAG TCACGACAAA GTCTTCCACT CCGTTCCAAA GCCCTCGTGC GCCATGCTTG 
CCAGTTCCTC CACCATTCAT CATGGAAGAA ACCCCAGAAA TGGGCTCAGA CAGCCTAACA 
GGGGCATTTG AGGCAGGGGT GGTGGAATGC TGCCCAAAGG AGGAATCAAC CAACCTAGAA 
GATGGAGAAG ATTCTGATAT TCCTACTCCT AAGTTGTTCC GAGACCCCCT GCAAGATGAT 
ACTGGGATGT TTGTGGCTGA AGAAGTTGCT TCTTCAGATG ACCATCTCAA GTCCATCTCT 
GCTATCTTCA AACAGGAACT TAAGGACATC ATCCTGTGCA TTTCACCTTT CATCACATTT 
GAGAAACCAT TTCTTCTTAC CCCTGCTGGG ATGCACTGCC CGAGCAGGGA TTATTTTCCC 
GAACAGGTCT ATCTGTCTCC TCTCCTCAAC AAGGACTTTA AAGAGTTGGA TGGACGCAGA 
AAGCGGCAGC TCCTTAAGGA GTCCACTCCC TCCCTGGCAA ACAGCCTAAC GAATGGTGGC 
CCCCCGTCAA AGCATGTCAG TGTCCTGCCA TCCCACAGTC TCACCAGCAC CCGGATCGTT 
GAACTAGTAA ACAGGAGCCA GGGTCTGGCC AGAATGGTTG CAGACTACAG AGCAAAGGGT 
GGACGTATCC AGCACAGCGA AGCTACTGAT CCATTTTGCA TAAGCAGCTC TTCTGCTCCG 
GTCAGTGGCC AGAATAGACC AGGTGATGGT TCGGTCAGAG CACCAGTAAA GACTGACAGC 
GAAGAGGAGA AGCCAACTAA ACCCAGTGAC TGGACCCCCA AGCTGGATTG TCTGAACCCC 
ACCAACCACC AGAGACTCTG TGTGCTTTTT AGCAGTTCAT CAACTCAGTC CAGCAATGCC 
CCCAACCCCT GTGTCAACCC ATGGATTGTT ACCATGGAGT TTTATGGCAA GAATGACCTC 
TGCCTTGGTG TATTCTTGGA GCGATATTGC TTTAGGCCAT CATACCAGTG CCCCAGTATA 
TTTTGTGAGA CCCCCATGGT GCACCACATC CGGCGTTTTG TCCATGGCAA CGGCTGCGTG 
CAGATAGTAC TGAAGGAGCT GGACTCCCCT GTGCCGGGGT ACCAGCACAC CATTCTCAAC 
TACTCCTGGT GCCGCATCTG CAAACAAGTG ACACCAGTGG TTCCTCTATC CAATGACTCA 
TGGTCAATGT CTTTTGCAAA ATATCTGGAG CTTCGCTTCT ACGGCCACCA GTACACCAGG 
AGGGCCAATG CAGAGCCCTG TGGCCACTCC ATCCACAAAG ACTACCACCA GTACTTCTCA 
TACAACCAAA TGGTGGCTTC CTTCAGTTAC ACCTCAGTGA GGCTGCTGGA GATTTGTCTT 
CCTCGTTTCA AAATCTTCAT CAGGAATCAG GGTCCTTCGA TCAACAACTT GGGGCAGGAT 
CTGAAAGACT TCTCTCAAAA AGTAAATCAG GTTTACGTGG CCATAGATGA TCGTCTCACA 
TCTCTAAAGA CGGACACCTT CAGTAAAACA CGCGAGGAGA AGATGGAGGA CCTCTTTGCA 
CAGAAAGATA TGGAAGAGGC GGAACTGCGC AGCTGGATTG AAAAGTTTCA AGCACGATTG 
CAGGCTTGTG GTCACGTTTC TCCTCAGCAG CTGCAGACGA CTCTGGAATC ACTGGTGATG 
AAGAAACAGA GTCTGTGTGA GATGTTGCAG TACTGGAACA GCAGGCTGCA GGAATTATTC 
CAACAGGAGA AAGGCAGGAA ACGTCTGTCA GTGCCCCCAA GCCCCGGCAG GCACAGACAG 
ACTGCCCCTG ATGATGGCAA GAACGCTGTG GAGTCATCCC CACGTAACCC TTCACCTGTG 
GTACAGAATG GTGAAAAGGA GGACCGTCAC CTCACCACAC TGCCTTCAAC CTCCTCTCTG 
TTGCCCTCGC CTGGAGACCT GGGAGCTGAA CCAGCCACAT CTGGTCCTTC CCTGAGTGAG 
CAGGACTCTG TCAGCATCCC AGAAGATGTA TTTGATGGAC ACTTGCTGGG CTCCAATGAC 
AGTCAGGTGA AGGAGAAATC CACCATGAAA GCCATCCTTG CCAACTTCCT GCCCGGCAAC 
AGTTACAACC CCATTCCATT CCCATTTGAC CCAGACAAAC ACTACCTGAT GGATGAGTAT 
GAGCGAGTGC CAATTGCAGT GTGTGAAAGA GAACCAAGCT CCATCATTGC ATTTGCTCTG 
AGTTGTAAGA AGTATAAAAC AGCACTGGAA GATCTGTCCA AGGTGTCAAA CATGGGTGGA 
GAAGACTCTT CACAAGCTGC CAGTAGTGGA GAGAGTCGGG TGAGGGGCAG CCCCGCCAGG 
CCCAGTGAGT CAGCCTCGTC CCAACAGAGC CGCAGCAGCA GTGAAGCAGA TCCCCTCAGT 
AGGCGTCCAG AAGACGCAGA CCTAACAGAC AAACAGAAGA AGCAGGCCCT GAATCCCCAC 
ATTGAGCTAC AGTTCTCTGA TGCAAGCGCA AAGTTTTACT GTCGGATCTA CTACGCCGAG 
GAGTTTCATA AGATGCGTGA GGAGATCATG GAGAGCTCCG AAGAGGACTT TGTGCGTTCC 
CTGTCGCACT GTGTCAACTG GCAGGCGCGC GGTGGAAAGT CTGGGGCCGT TTTCTACGCA 
ACAGAAGACG ACCGTTTCAT CCTAAAACAG ATGCCCAGGC TGGAGGTGCA GTCCTTCTTG 
GACTTTGCTC CGCACTACTT CACCTACATC ACCGGAGCTG TGCAGCAGAA GAGGCCAACT 
GCATTGGCAA AGATTCTCGG AGTTTACAGG ATAGGCTACA AGAACTCCCA GAACAACACT 
GAGAAGAAAC TGGACCTGCT CGTCATGGAA AACCTGTTCT ACGGACGCAA GATGGCTCAG 
GTCTTTGACC TGAAAGGTTC CCTCAGAAAC CGTCATGTGA AGACAGACCT GGTAAAGGAA 
AGCTGCGAGG TGGTCCTGCT GGATGAGAAT CTACTGAAGC TGATCCACGA CAATCCGCTC 
TACATTCGCT CTCACTGCAA GTCCATCTTG CGCGCCGCCA TACACAGCGA CGCCTACTTC 
CTGTCCAGCC ACCTCATCAT TGACTACTCC CTGCTGGTGG GGTGGGACGA TGCCACCGAC 
CAGCTGGTGG TTGGGATCAT AGACTATATT CGGACGTTTA CCTGGGACAA GAAATTAGAG 
ATGGTTGTCA AATCCACTGG AATCCTGGGG GGTCAGGGAA AGATGCCCAC CGTTGTTTCT 
CCTGAGCTGT ACCGAGCTCG GTTCTGTGAG GCCATGGACA AATACTTCCT GATGGTACCC 
GACCACTGGA CGGGTCTTGG CATCAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688502.1
CDS	131..6403
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKEEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832642.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832642.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGAGGAG GGACGTTCTC TTTCAACAGT GTCAGAGAAA 
TCAGACACTC CATCCCCTTC CCCTCTATCA GAGAGGAGGA GCTGGTCCTC CAGCCCACCC 
CATTCCCTCT ACACTGCCAG GATTCCCCGA AAACAACCAG ATCACCTTCA AAGGACCTCT 
GCTGCTTCAG ATGGCAGCAG GAAATCAGAG ACTCCTTTAA GCAATCACGA CCCTCGAACT 
GCTGTGCAAC TTCGCACTGC ATTGAAGAGG CTGAAGGAAA TAATGGAGGG AAAGAGCCAG 
GACAGTGACT TGAAGCAGTA CTGGATGCCG GACAGCCAGT GTAAAGAATG TTATGACTGC 
AATGAAAAGT TCACAACCTT CCGACGCCGC CACCATTGCC GACTGTGTGG TCAGATTTTC 
TGCAGTCGCT GCTGCAACCA GGAAATCCCT GGAAAGTTCA TGGGTTACAC AGGAGATTTG 
CGGGCCTGTA CGTACTGCCG TAAGTTGGCA CTGAGCTACG CCCACTCGGC TGATTCGGGC 
TCCATCGGCG AAGACTTGAA CACCCTGACT GACTCTCCGA GCTCTGTGTG TGTGCTGGAG 
CCCAGTGAGC CACGGACGCC TGTGGGGGGG CGCAAGGCCA GCAGGAACAT CTTCCTAGAG 
GAAGACTTAA CATGGCAGAG AAAAACACCT ATTGGGATGA GAAAGAACAT GATTCACCAG 
GAACCTCAGA GCAGCGGCCT CAGTTCCAGA CTGACAGGAC TTCAGGAGGA TGTAGGAAAA 
TCACCTGGTA GGAGGAGGTC TGCTAGCATC AGCACACTTC CACTTGACCG CTCTGGGTCA 
TCAATGGTAC CTTCCTACGA CAGCTCCGTC AGCCCCCCTA CCAGCAGAGC CATGTCGGTG 
TCAAAGGCTG GGACAAAATT AGATCACAGT GAGGAGGAAC GTAAAATACT ACTGGACTCC 
TCCCAGTTGA AGGACTTATG GAGGAAAATC TGTCATAATG GAACAGGGAT GGAATTCCAG 
GACCACAGAT ACTGGCTGAG AACCTACCCC AACTGCATTG TGGGAAAAGA GCTCGTCAAC 
TGGCTGCTAA GAAATGGCAC CATTTCCAAC AGGGTCCAAG CTATTGCCAT AGGTCAGGCA 
CTCGTGGATG GCCGCTGGCT CGACTGTGTC ACCCACCACG ATCAGCTATT CAGAGATGAG 
TATGCTCTCT ATCGACCACT TCAGAGTACA GAGTTCTCAG AAACTCCCTC CCCGGACAGC 
GATAGTGTCA ACTCACTAGA GGGACATTCA GAGCCGTCAT GGTTCAAGGA CATCAAATTC 
GATGACAGCG ACACCGAGCA GCCCGCAGAC GACACAGAGT ACACCATGCC TAGTTCCGCT 
GGGCCCAGCA AGAGGACATC TGTCAGCAGC GTCCACTCAG CAGTAGACAG TGACTCAGCT 
GCCTCCATCA ACCTCAACAT GGAGCAAAAC AATGTGAATT TTCGAATCAA GAAAGATTCC 
AAGTGTCCCC AGGCATTCCC CGCCCTGGAG CACAAATCTG AAATTATGGC GTCTGAAGAT 
GGAGGAGAGA ACATCCTTAT AAGTGATGCC TTTATCAAAG AGTCTCTCTT TAACCGTCGT 
GTGGAGGAGA AGGCTAAAGA AATGCTGTTT ACTCCACTGG GTTGGCACCA CAGCTCTCTG 
GACCAGCTCA GAGAGGAGAA TGGAGAAAAG AAGGCTATGG AGAGGCTGCT CTCGGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAG TTGCTCTACA GCGAGTCTTT GTCTCTGTCC 
TGGCGGGATA TTATCGTTCC TGTGGTTCGC CAGGTTGTCC AGACAGTTCG ACCTGATGTT 
CGCAACAGTG ATGACGACAT GGACATCCGC CAACTGGTCC ATATCAAAAA GATTCCTGGA 
GGAAAAAAGT TTGACTCAAC TGTAGTGAAT GGCTTTGTGT GTACGAAGAG TATTGCCCAC 
AAAAAGATGA ACCCATATAT CAAGAACCCC AAAATTCTGC TGCTGAAATG CTCAATAGAG 
TATCTGTACA GGGAAGAGAC CAAATTCACG TCCATCGACC CCATCGTGCT GCAGGAACGA 
GAGTTTCTGA AAAACTATGT GCAACGTATC GTGGATGTTC GGCCCACTCT GGTTCTGGTG 
GAAAAAACGG TGTCTCGTAT AGCTCAGGAC ATGCTGCTAG AACACGGCAT TACCCTGGTC 
ATTAATGTCA AACCGCAAGT ATTGGACAGG GTGAGTTGTG TGACCCAGGG GGACCTTGTC 
ATGTCCATGG ACCAGCTACT CACCAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACATG 
CAGCCCTTCC CTTTGGCCAA TAATGAGGTC AAGACTCTGA TGTTCTTCGA AGGATGTCCT 
GCTCATCTCG GGTGCTCCAT AAATCTACGT GGTGCTTCAG ACTACGAGTT AGCCAGGGTG 
AAGGAGATCA TGCGATTAAT GGTGTGTGTG GCCTACCACT CTCAGCTGGA GATCTCCTTC 
CTCATGGATG AGTTTGCCAT GCCGCCCAGC TTGTCCCAGA GCACTTCATT TCCCTGTCTC 
CTCGACGGAG GCTCTGCAGA TGAGGAAGCA AGCAGGGAGA AACTAGAGAA GAGTTCTGAA 
GATGAAGACA CTGTAGTGGA AGGACAACCT GAGGAGGTGC CCTCTGCCAA TGATGTAGAC 
ACTTCCCAGG ACACAAATGA TCCTAAATTA TTCTCCTCTA TGGTGGAAGA GACCACTAAT 
ACTGACACAG TCACGACAAA GTCTTCCACT CCGTTCCAAA GCCCTCGTGC GCCATGCTTG 
CCAGTTCCTC CACCATTCAT CATGGAAGAA ACCCCAGAAA TGGGCTCAGA CAGCCTAACA 
GGGGCATTTG AGGCAGGGGT GGTGGAATGC TGCCCAAAGG AGGAATCAAC CAACCTAGAA 
GATGGAGAAG ATTCTGATAT TCCTACTCCT AAGTTGTTCC GAGACCCCCT GCAAGATGAT 
ACTGGGATGT TTGTGGCTGA AGAAGTTGCT TCTTCAGATG ACCATCTCAA GTCCATCTCT 
GCTATCTTCA AACAGGAACT TAAGGACATC ATCCTGTGCA TTTCACCTTT CATCACATTT 
GAGAAACCAT TTCTTCTTAC CCCTGCTGGG ATGCACTGCC CGAGCAGGGA TTATTTTCCC 
GAACAGGTCT ATCTGTCTCC TCTCCTCAAC AAGGACTTTA AAGAGTTGGA TGGACGCAGA 
AAGCGGCAGC TCCTTAAGGA GTCCACTCCC TCCCTGGCAA ACAGCCTAAC GAATGGTGGC 
CCCCCGTCAA AGCATGTCAG TGTCCTGCCA TCCCACAGTC TCACCAGCAC CCGGATCGTT 
GAACTAGTAA ACAGGAGCCA GGGTCTGGCC AGAATGGTTG CAGACTACAG AGCAAAGGGT 
GGACGTATCC AGCACAGCGA AGCTACTGAT CCATTTTGCA TAAGCAGCTC TTCTGCTCCG 
GTCAGTGGCC AGAATAGACC AGGTGATGGT TCGGTCAGAG CACCAGTAAA GACTGACAGC 
GAAGAGGAGA AGCCAACTAA ACCCAGTGAC TGGACCCCCA AGCTGGATTG TCTGAACCCC 
ACCAACCACC AGAGACTCTG TGTGCTTTTT AGCAGTTCAT CAACTCAGTC CAGCAATGCC 
CCCAACCCCT GTGTCAACCC ATGGATTGTT ACCATGGAGT TTTATGGCAA GAATGACCTC 
TGCCTTGGTG TATTCTTGGA GCGATATTGC TTTAGGCCAT CATACCAGTG CCCCAGTATA 
TTTTGTGAGA CCCCCATGGT GCACCACATC CGGCGTTTTG TCCATGGCAA CGGCTGCGTG 
CAGATAGTAC TGAAGGAGCT GGACTCCCCT GTGCCGGGGT ACCAGCACAC CATTCTCAAC 
TACTCCTGGT GCCGCATCTG CAAACAAGTG ACACCAGTGG TTCCTCTATC CAATGACTCA 
TGGTCAATGT CTTTTGCAAA ATATCTGGAG CTTCGCTTCT ACGGCCACCA GTACACCAGG 
AGGGCCAATG CAGAGCCCTG TGGCCACTCC ATCCACAAAG ACTACCACCA GTACTTCTCA 
TACAACCAAA TGGTGGCTTC CTTCAGTTAC ACCTCAGTGA GGCTGCTGGA GATTTGTCTT 
CCTCGTTTCA AAATCTTCAT CAGGAATCAG GGTCCTTCGA TCAACAACTT GGGGCAGGAT 
CTGAAAGACT TCTCTCAAAA AGTAAATCAG GTTTACGTGG CCATAGATGA TCGTCTCACA 
TCTCTAAAGA CGGACACCTT CAGTAAAACA CGCGAGGAGA AGATGGAGGA CCTCTTTGCA 
CAGAAAGATA TGGAAGAGGC GGAACTGCGC AGCTGGATTG AAAAGTTTCA AGCACGATTG 
CAGGCTTGTG GTCACGTTTC TCCTCAGCAG CTGCAGACGA CTCTGGAATC ACTGGTGATG 
AAGAAACAGA GTCTGTGTGA GATGTTGCAG TACTGGAACA GCAGGCTGCA GGAATTATTC 
CAACAGGAGA AAGGCAGGAA ACGTCTGTCA GTGCCCCCAA GCCCCGGCAG GCACAGACAG 
ACTGCCCCTG ATGATGGCAA GAACGCTGTG GAGTCATCCC CACGTAACCC TTCACCTGTG 
GTACAGAATG GTGAAAAGGA GGACCGTCAC CTCACCACAC TGCCTTCAAC CTCCTCTCTG 
TTGCCCTCGC CTGGAGACCT GGGAGCTGAA CCAGCCACAT CTGGTCCTTC CCTGAGTGAG 
CAGGACTCTG TCAGCATCCC AGAAGATGTA TTTGATGGAC ACTTGCTGGG CTCCAATGAC 
AGTCAGGTGA AGGAGAAATC CACCATGAAA GCCATCCTTG CCAACTTCCT GCCCGGCAAC 
AGTTACAACC CCATTCCATT CCCATTTGAC CCAGACAAAC ACTACCTGAT GGATGAGTAT 
GAGCGAGTGC CAATTGCAGT GTGTGAAAGA GAACCAAGCT CCATCATTGC ATTTGCTCTG 
AGTTGTAAGA AGTATAAAAC AGCACTGGAA GATCTGTCCA AGGTGTCAAA CATGGGTGGA 
GAAGACTCTT CACAAGCTGC CAGTAGTGGA GAGAGTCGGG TGAGGGGCAG CCCCGCCAGG 
CCCAGTGAGT CAGCCTCGTC CCAACAGAGC CGCAGCAGCA GTGAAGCAGA TCCCCTCAGT 
AGGCGTCCAG AAGACGCAGA CCTAACAGAC AAACAGAAGA AGCAGGCCCT GAATCCCCAC 
ATTGAGCTAC AGTTCTCTGA TGCAAGCGCA AAGTTTTACT GTCGGATCTA CTACGCCGAG 
GAGTTTCATA AGATGCGTGA GGAGATCATG GAGAGCTCCG AAGAGGACTT TGTGCGTTCC 
CTGTCGCACT GTGTCAACTG GCAGGCGCGC GGTGGAAAGT CTGGGGCCGT TTTCTACGCA 
ACAGAAGACG ACCGTTTCAT CCTAAAACAG ATGCCCAGGC TGGAGGTGCA GTCCTTCTTG 
GACTTTGCTC CGCACTACTT CACCTACATC ACCGGAGCTG TGCAGCAGAA GAGGCCAACT 
GCATTGGCAA AGATTCTCGG AGTTTACAGG ATAGGCTACA AGAACTCCCA GAACAACACT 
GAGAAGAAAC TGGACCTGCT CGTCATGGAA AACCTGTTCT ACGGACGCAA GATGGCTCAG 
GTCTTTGACC TGAAAGGTTC CCTCAGAAAC CGTCATGTGA AGACAGACCT GGTAAAGGAA 
AGCTGCGAGG TGGTCCTGCT GGATGAGAAT CTACTGAAGC TGATCCACGA CAATCCGCTC 
TACATTCGCT CTCACTGCAA GTCCATCTTG CGCGCCGCCA TACACAGCGA CGCCTACTTC 
CTGTCCAGCC ACCTCATCAT TGACTACTCC CTGCTGGTGG GGTGGGACGA TGCCACCGAC 
CAGCTGGTGG TTGGGATCAT AGACTATATT CGGACGTTTA CCTGGGACAA GAAATTAGAG 
ATGGTTGTCA AATCCACTGG AATCCTGGGG GGTCAGGGAA AGATGCCCAC CGTTGTTTCT 
CCTGAGCTGT ACCGAGCTCG GTTCTGTGAG GCCATGGACA AATACTTCCT GATGGTACCC 
GACCACTGGA CGGGTCTTGG CATCAACTGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832644.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688504.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X16, mRNA.

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CloneID	OTo40934
Clone ID Related Accession (Same CDS sequence)	XM_029832644.1
Accession Version	XM_029832644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGAGGAG GGACGTTCTC TTTCAACAGT GTCAGAGAAA 
TCAGACACTC CATCCCCTTC CCCTCTATCA GAGAGGAGGA GCTGGTCCTC CAGCCCACCC 
CATTCCCTCT ACACTGCCAG GATTCCCCGA AAACAACCAG ATCACCTTCA AAGGACCTCT 
GCTGCTTCAG ATGGCAGCAG GAAATCAGAG ACTCCTTTAA GCAATCACGA CCCTCGAACT 
GCTGTGCAAC TTCGCACTGC ATTGAAGAGG CTGAAGGAAA TAATGGAGGG AAAGAGCCAG 
GACAGTGACT TGAAGCAGTA CTGGATGCCG GACAGCCAGT GTAAAGAATG TTATGACTGC 
AATGAAAAGT TCACAACCTT CCGACGCCGC CACCATTGCC GACTGTGTGG TCAGATTTTC 
TGCAGTCGCT GCTGCAACCA GGAAATCCCT GGAAAGTTCA TGGGTTACAC AGGAGATTTG 
CGGGCCTGTA CGTACTGCCG TAAGTTGGCA CTGAGCTACG CCCACTCGGC TGATTCGGGC 
TCCATCGGCG AAGACTTGAA CACCCTGACT GACTCTCCGA GCTCTGTGTG TGTGCTGGAG 
CCCAGTGAGC CACGGACGCC TGTGGGGGGG CGCAAGGCCA GCAGGAACAT CTTCCTAGAG 
GAAGACTTAA CATGGCAGAG AAAAACACCT ATTGGGATGA GAAAGAACAT GATTCACCAG 
GAACCTCAGA GCAGCGGCCT CAGTTCCAGA CTGACAGGAC TTCAGGAGGA TGTAGGAAAA 
TCACCTGGTA GGAGGAGGTC TGCTAGCATC AGCACACTTC CACTTGACCG CTCTGGGTCA 
TCAATGGTAC CTTCCTACGA CAGCTCCGTC AGCCCCCCTA CCAGCAGAGC CATGTCGGTG 
TCAAAGGCTG GGACAAAATT AGATCACAGT GAGGAGGAAC GTAAAATACT ACTGGACTCC 
TCCCAGTTGA AGGACTTATG GAGGAAAATC TGTCATAATG GAACAGGGAT GGAATTCCAG 
GACCACAGAT ACTGGCTGAG AACCTACCCC AACTGCATTG TGGGAAAAGA GCTCGTCAAC 
TGGCTGCTAA GAAATGGCAC CATTTCCAAC AGGGTCCAAG CTATTGCCAT AGGTCAGGCA 
CTCGTGGATG GCCGCTGGCT CGACTGTGTC ACCCACCACG ATCAGCTATT CAGAGATGAG 
TATGCTCTCT ATCGACCACT TCAGAGTACA GAGTTCTCAG AAACTCCCTC CCCGGACAGC 
GATAGTGTCA ACTCACTAGA GGGACATTCA GAGCCGTCAT GGTTCAAGGA CATCAAATTC 
GATGACAGCG ACACCGAGCA GCCCGCAGAC GACACAGAGT ACACCATGCC TAGTTCCGCT 
GGGCCCAGCA AGAGGACATC TGTCAGCAGC GTCCACTCAG CAGTAGACAG TGACTCAGCT 
GCCTCCATCA ACCTCAACAT GGAGCAAAAC AATGTGAATT TTCGAATCAA GAAAGATTCC 
AAGTGTCCCC AGGCATTCCC CGCCCTGGAG CACAAATCTG AAATTATGGC GTCTGAAGAT 
GGAGGAGAGA ACATCCTTAT AAGTGATGCC TTTATCAAAG AGTCTCTCTT TAACCGTCGT 
GTGGAGGAGA AGGCTAAAGA AATGCTGTTT ACTCCACTGG GTTGGCACCA CAGCTCTCTG 
GACCAGCTCA GAGAGGAGAA TGGAGAAAAG AAGGCTATGG AGAGGCTGCT CTCGGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAG TTGCTCTACA GCGAGTCTTT GTCTCTGTCC 
TGGCGGGATA TTATCGTTCC TGTGGTTCGC CAGGTTGTCC AGACAGTTCG ACCTGATGTT 
CGCAACAGTG ATGACGACAT GGACATCCGC CAACTGGTCC ATATCAAAAA GATTCCTGGA 
GGAAAAAAGT TTGACTCAAC TGTAGTGAAT GGCTTTGTGT GTACGAAGAG TATTGCCCAC 
AAAAAGATGA ACCCATATAT CAAGAACCCC AAAATTCTGC TGCTGAAATG CTCAATAGAG 
TATCTGTACA GGGAAGAGAC CAAATTCACG TCCATCGACC CCATCGTGCT GCAGGAACGA 
GAGTTTCTGA AAAACTATGT GCAACGTATC GTGGATGTTC GGCCCACTCT GGTTCTGGTG 
GAAAAAACGG TGTCTCGTAT AGCTCAGGAC ATGCTGCTAG AACACGGCAT TACCCTGGTC 
ATTAATGTCA AACCGCAAGT ATTGGACAGG GTGAGTTGTG TGACCCAGGG GGACCTTGTC 
ATGTCCATGG ACCAGCTACT CACCAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACATG 
CAGCCCTTCC CTTTGGCCAA TAATGAGGTC AAGACTCTGA TGTTCTTCGA AGGATGTCCT 
GCTCATCTCG GGTGCTCCAT AAATCTACGT GGTGCTTCAG ACTACGAGTT AGCCAGGGTG 
AAGGAGATCA TGCGATTAAT GGTGTGTGTG GCCTACCACT CTCAGCTGGA GATCTCCTTC 
CTCATGGATG AGTTTGCCAT GCCGCCCAGC TTGTCCCAGA GCACTTCATT TCCCTGTCTC 
CTCGACGGAG GCTCTGCAGA TGAGGAAGCA AGCAGGGAGA AACTAGAGAA GAGTTCTGAA 
GATGAAGACA CTGTAGTGGA AGGACAACCT GAGGAGGTGC CCTCTGCCAA TGATGTAGAC 
ACTTCCCAGG ACACAAATGA TCCTAAATTA TTCTCCTCTA TGGTGGAAGA GACCACTAAT 
ACTGACACAG TCACGACAAA GTCTTCCACT CCGTTCCAAA GCCCTCGTGC GCCATGCTTG 
CCAGTTCCTC CACCATTCAT CATGGAAGAA ACCCCAGAAA TGGGCTCAGA CAGCCTAACA 
GGGGCATTTG AGGCAGGGGT GGTGGAATGC TGCCCAAAGG AGGAATCAAC CAACCTAGAA 
GATGGAGAAG ATTCTGATAT TCCTACTCCT AAGTTGTTCC GAGACCCCCT GCAAGATGAT 
ACTGGGATGT TTGTGGCTGA AGAAGTTGCT TCTTCAGATG ACCATCTCAA GTCCATCTCT 
GCTATCTTCA AACAGGAACT TAAGGACATC ATCCTGTGCA TTTCACCTTT CATCACATTT 
GAGAAACCAT TTCTTCTTAC CCCTGCTGGG ATGCACTGCC CGAGCAGGGA TTATTTTCCC 
GAACAGGTCT ATCTGTCTCC TCTCCTCAAC AAGGACTTTA AAGAGTTGGA TGGACGCAGA 
AAGCGGCAGC TCCTTAAGGA GTCCACTCCC TCCCTGGCAA ACAGCCTAAC GAATGGTGGC 
CCCCCGTCAA AGCATGTCAG TGTCCTGCCA TCCCACAGTC TCACCAGCAC CCGGATCGTT 
GAACTAGTAA ACAGGAGCCA GGGTCTGGCC AGAATGGTTG CAGACTACAG AGCAAAGGGT 
GGACGTATCC AGCACAGCGA AGCTACTGAT CCATTTTGCA TAAGCAGCTC TTCTGCTCCG 
GTCAGTGGCC AGAATAGACC AGGTGATGGT TCGGTCAGAG CACCAGTAAA GACTGACAGC 
GAAGAGGAGA AGCCAACTAA ACCCAGTGAC TGGACCCCCA AGCTGGATTG TCTGAACCCC 
ACCAACCACC AGAGACTCTG TGTGCTTTTT AGCAGTTCAT CAACTCAGTC CAGCAATGCC 
CCCAACCCCT GTGTCAACCC ATGGATTGTT ACCATGGAGT TTTATGGCAA GAATGACCTC 
TGCCTTGGTG TATTCTTGGA GCGATATTGC TTTAGGCCAT CATACCAGTG CCCCAGTATA 
TTTTGTGAGA CCCCCATGGT GCACCACATC CGGCGTTTTG TCCATGGCAA CGGCTGCGTG 
CAGATAGTAC TGAAGGAGCT GGACTCCCCT GTGCCGGGGT ACCAGCACAC CATTCTCAAC 
TACTCCTGGT GCCGCATCTG CAAACAAGTG ACACCAGTGG TTCCTCTATC CAATGACTCA 
TGGTCAATGT CTTTTGCAAA ATATCTGGAG CTTCGCTTCT ACGGCCACCA GTACACCAGG 
AGGGCCAATG CAGAGCCCTG TGGCCACTCC ATCCACAAAG ACTACCACCA GTACTTCTCA 
TACAACCAAA TGGTGGCTTC CTTCAGTTAC ACCTCAGTGA GGCTGCTGGA GATTTGTCTT 
CCTCGTTTCA AAATCTTCAT CAGGAATCAG GGTCCTTCGA TCAACAACTT GGGGCAGGAT 
CTGAAAGACT TCTCTCAAAA AGTAAATCAG GTTTACGTGG CCATAGATGA TCGTCTCACA 
TCTCTAAAGA CGGACACCTT CAGTAAAACA CGCGAGGAGA AGATGGAGGA CCTCTTTGCA 
CAGAAAGATA TGGAAGAGGC GGAACTGCGC AGCTGGATTG AAAAGTTTCA AGCACGATTG 
CAGGCTTGTG GTCACGTTTC TCCTCAGCAG CTGCAGACGA CTCTGGAATC ACTGGTGATG 
AAGAAACAGA GTCTGTGTGA GATGTTGCAG TACTGGAACA GCAGGCTGCA GGAATTATTC 
CAACAGGAGA AAGGCAGGAA ACGTCTGTCA GTGCCCCCAA GCCCCGGCAG GCACAGACAG 
ACTGCCCCTG ATGATGGCAA GAACGCTGTG GAGTCATCCC CACGTAACCC TTCACCTGTG 
GTACAGAATG GTGAAAAGGA GGACCGTCAC CTCACCACAC TGCCTTCAAC CTCCTCTCTG 
TTGCCCTCGC CTGGAGACCT GGGAGCTGAA CCAGCCACAT CTGGTCCTTC CCTGAGTGAG 
CAGGACTCTG TCAGCATCCC AGAAGATGTA TTTGATGGAC ACTTGCTGGG CTCCAATGAC 
AGTCAGGTGA AGGAGAAATC CACCATGAAA GCCATCCTTG CCAACTTCCT GCCCGGCAAC 
AGTTACAACC CCATTCCATT CCCATTTGAC CCAGACAAAC ACTACCTGAT GGATGAGTAT 
GAGCGAGTGC CAATTGCAGT GTGTGAAAGA GAACCAAGCT CCATCATTGC ATTTGCTCTG 
AGTTGTAAGA AGTATAAAAC AGCACTGGAA GATCTGTCCA AGGTGTCAAA CATGGGTGGA 
GAAGACTCTT CACAAGCTGC CAGTAGTGGA GAGAGTCGGG TGAGGGGCAG CCCCGCCAGG 
CCCAGTGAGT CAGCCTCGTC CCAACAGAGC CGCAGCAGCA GTGAAGCAGA TCCCCTCAAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688504.1
CDS	128..6388
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKEEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832644.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832644.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGAGGAG GGACGTTCTC TTTCAACAGT GTCAGAGAAA 
TCAGACACTC CATCCCCTTC CCCTCTATCA GAGAGGAGGA GCTGGTCCTC CAGCCCACCC 
CATTCCCTCT ACACTGCCAG GATTCCCCGA AAACAACCAG ATCACCTTCA AAGGACCTCT 
GCTGCTTCAG ATGGCAGCAG GAAATCAGAG ACTCCTTTAA GCAATCACGA CCCTCGAACT 
GCTGTGCAAC TTCGCACTGC ATTGAAGAGG CTGAAGGAAA TAATGGAGGG AAAGAGCCAG 
GACAGTGACT TGAAGCAGTA CTGGATGCCG GACAGCCAGT GTAAAGAATG TTATGACTGC 
AATGAAAAGT TCACAACCTT CCGACGCCGC CACCATTGCC GACTGTGTGG TCAGATTTTC 
TGCAGTCGCT GCTGCAACCA GGAAATCCCT GGAAAGTTCA TGGGTTACAC AGGAGATTTG 
CGGGCCTGTA CGTACTGCCG TAAGTTGGCA CTGAGCTACG CCCACTCGGC TGATTCGGGC 
TCCATCGGCG AAGACTTGAA CACCCTGACT GACTCTCCGA GCTCTGTGTG TGTGCTGGAG 
CCCAGTGAGC CACGGACGCC TGTGGGGGGG CGCAAGGCCA GCAGGAACAT CTTCCTAGAG 
GAAGACTTAA CATGGCAGAG AAAAACACCT ATTGGGATGA GAAAGAACAT GATTCACCAG 
GAACCTCAGA GCAGCGGCCT CAGTTCCAGA CTGACAGGAC TTCAGGAGGA TGTAGGAAAA 
TCACCTGGTA GGAGGAGGTC TGCTAGCATC AGCACACTTC CACTTGACCG CTCTGGGTCA 
TCAATGGTAC CTTCCTACGA CAGCTCCGTC AGCCCCCCTA CCAGCAGAGC CATGTCGGTG 
TCAAAGGCTG GGACAAAATT AGATCACAGT GAGGAGGAAC GTAAAATACT ACTGGACTCC 
TCCCAGTTGA AGGACTTATG GAGGAAAATC TGTCATAATG GAACAGGGAT GGAATTCCAG 
GACCACAGAT ACTGGCTGAG AACCTACCCC AACTGCATTG TGGGAAAAGA GCTCGTCAAC 
TGGCTGCTAA GAAATGGCAC CATTTCCAAC AGGGTCCAAG CTATTGCCAT AGGTCAGGCA 
CTCGTGGATG GCCGCTGGCT CGACTGTGTC ACCCACCACG ATCAGCTATT CAGAGATGAG 
TATGCTCTCT ATCGACCACT TCAGAGTACA GAGTTCTCAG AAACTCCCTC CCCGGACAGC 
GATAGTGTCA ACTCACTAGA GGGACATTCA GAGCCGTCAT GGTTCAAGGA CATCAAATTC 
GATGACAGCG ACACCGAGCA GCCCGCAGAC GACACAGAGT ACACCATGCC TAGTTCCGCT 
GGGCCCAGCA AGAGGACATC TGTCAGCAGC GTCCACTCAG CAGTAGACAG TGACTCAGCT 
GCCTCCATCA ACCTCAACAT GGAGCAAAAC AATGTGAATT TTCGAATCAA GAAAGATTCC 
AAGTGTCCCC AGGCATTCCC CGCCCTGGAG CACAAATCTG AAATTATGGC GTCTGAAGAT 
GGAGGAGAGA ACATCCTTAT AAGTGATGCC TTTATCAAAG AGTCTCTCTT TAACCGTCGT 
GTGGAGGAGA AGGCTAAAGA AATGCTGTTT ACTCCACTGG GTTGGCACCA CAGCTCTCTG 
GACCAGCTCA GAGAGGAGAA TGGAGAAAAG AAGGCTATGG AGAGGCTGCT CTCGGCAAAT 
CACAGCCACA TGATGGCGCT GCTGCAGCAG TTGCTCTACA GCGAGTCTTT GTCTCTGTCC 
TGGCGGGATA TTATCGTTCC TGTGGTTCGC CAGGTTGTCC AGACAGTTCG ACCTGATGTT 
CGCAACAGTG ATGACGACAT GGACATCCGC CAACTGGTCC ATATCAAAAA GATTCCTGGA 
GGAAAAAAGT TTGACTCAAC TGTAGTGAAT GGCTTTGTGT GTACGAAGAG TATTGCCCAC 
AAAAAGATGA ACCCATATAT CAAGAACCCC AAAATTCTGC TGCTGAAATG CTCAATAGAG 
TATCTGTACA GGGAAGAGAC CAAATTCACG TCCATCGACC CCATCGTGCT GCAGGAACGA 
GAGTTTCTGA AAAACTATGT GCAACGTATC GTGGATGTTC GGCCCACTCT GGTTCTGGTG 
GAAAAAACGG TGTCTCGTAT AGCTCAGGAC ATGCTGCTAG AACACGGCAT TACCCTGGTC 
ATTAATGTCA AACCGCAAGT ATTGGACAGG GTGAGTTGTG TGACCCAGGG GGACCTTGTC 
ATGTCCATGG ACCAGCTACT CACCAAACCT CGTCTGGGTA CCTGCCACAA GTTTTACATG 
CAGCCCTTCC CTTTGGCCAA TAATGAGGTC AAGACTCTGA TGTTCTTCGA AGGATGTCCT 
GCTCATCTCG GGTGCTCCAT AAATCTACGT GGTGCTTCAG ACTACGAGTT AGCCAGGGTG 
AAGGAGATCA TGCGATTAAT GGTGTGTGTG GCCTACCACT CTCAGCTGGA GATCTCCTTC 
CTCATGGATG AGTTTGCCAT GCCGCCCAGC TTGTCCCAGA GCACTTCATT TCCCTGTCTC 
CTCGACGGAG GCTCTGCAGA TGAGGAAGCA AGCAGGGAGA AACTAGAGAA GAGTTCTGAA 
GATGAAGACA CTGTAGTGGA AGGACAACCT GAGGAGGTGC CCTCTGCCAA TGATGTAGAC 
ACTTCCCAGG ACACAAATGA TCCTAAATTA TTCTCCTCTA TGGTGGAAGA GACCACTAAT 
ACTGACACAG TCACGACAAA GTCTTCCACT CCGTTCCAAA GCCCTCGTGC GCCATGCTTG 
CCAGTTCCTC CACCATTCAT CATGGAAGAA ACCCCAGAAA TGGGCTCAGA CAGCCTAACA 
GGGGCATTTG AGGCAGGGGT GGTGGAATGC TGCCCAAAGG AGGAATCAAC CAACCTAGAA 
GATGGAGAAG ATTCTGATAT TCCTACTCCT AAGTTGTTCC GAGACCCCCT GCAAGATGAT 
ACTGGGATGT TTGTGGCTGA AGAAGTTGCT TCTTCAGATG ACCATCTCAA GTCCATCTCT 
GCTATCTTCA AACAGGAACT TAAGGACATC ATCCTGTGCA TTTCACCTTT CATCACATTT 
GAGAAACCAT TTCTTCTTAC CCCTGCTGGG ATGCACTGCC CGAGCAGGGA TTATTTTCCC 
GAACAGGTCT ATCTGTCTCC TCTCCTCAAC AAGGACTTTA AAGAGTTGGA TGGACGCAGA 
AAGCGGCAGC TCCTTAAGGA GTCCACTCCC TCCCTGGCAA ACAGCCTAAC GAATGGTGGC 
CCCCCGTCAA AGCATGTCAG TGTCCTGCCA TCCCACAGTC TCACCAGCAC CCGGATCGTT 
GAACTAGTAA ACAGGAGCCA GGGTCTGGCC AGAATGGTTG CAGACTACAG AGCAAAGGGT 
GGACGTATCC AGCACAGCGA AGCTACTGAT CCATTTTGCA TAAGCAGCTC TTCTGCTCCG 
GTCAGTGGCC AGAATAGACC AGGTGATGGT TCGGTCAGAG CACCAGTAAA GACTGACAGC 
GAAGAGGAGA AGCCAACTAA ACCCAGTGAC TGGACCCCCA AGCTGGATTG TCTGAACCCC 
ACCAACCACC AGAGACTCTG TGTGCTTTTT AGCAGTTCAT CAACTCAGTC CAGCAATGCC 
CCCAACCCCT GTGTCAACCC ATGGATTGTT ACCATGGAGT TTTATGGCAA GAATGACCTC 
TGCCTTGGTG TATTCTTGGA GCGATATTGC TTTAGGCCAT CATACCAGTG CCCCAGTATA 
TTTTGTGAGA CCCCCATGGT GCACCACATC CGGCGTTTTG TCCATGGCAA CGGCTGCGTG 
CAGATAGTAC TGAAGGAGCT GGACTCCCCT GTGCCGGGGT ACCAGCACAC CATTCTCAAC 
TACTCCTGGT GCCGCATCTG CAAACAAGTG ACACCAGTGG TTCCTCTATC CAATGACTCA 
TGGTCAATGT CTTTTGCAAA ATATCTGGAG CTTCGCTTCT ACGGCCACCA GTACACCAGG 
AGGGCCAATG CAGAGCCCTG TGGCCACTCC ATCCACAAAG ACTACCACCA GTACTTCTCA 
TACAACCAAA TGGTGGCTTC CTTCAGTTAC ACCTCAGTGA GGCTGCTGGA GATTTGTCTT 
CCTCGTTTCA AAATCTTCAT CAGGAATCAG GGTCCTTCGA TCAACAACTT GGGGCAGGAT 
CTGAAAGACT TCTCTCAAAA AGTAAATCAG GTTTACGTGG CCATAGATGA TCGTCTCACA 
TCTCTAAAGA CGGACACCTT CAGTAAAACA CGCGAGGAGA AGATGGAGGA CCTCTTTGCA 
CAGAAAGATA TGGAAGAGGC GGAACTGCGC AGCTGGATTG AAAAGTTTCA AGCACGATTG 
CAGGCTTGTG GTCACGTTTC TCCTCAGCAG CTGCAGACGA CTCTGGAATC ACTGGTGATG 
AAGAAACAGA GTCTGTGTGA GATGTTGCAG TACTGGAACA GCAGGCTGCA GGAATTATTC 
CAACAGGAGA AAGGCAGGAA ACGTCTGTCA GTGCCCCCAA GCCCCGGCAG GCACAGACAG 
ACTGCCCCTG ATGATGGCAA GAACGCTGTG GAGTCATCCC CACGTAACCC TTCACCTGTG 
GTACAGAATG GTGAAAAGGA GGACCGTCAC CTCACCACAC TGCCTTCAAC CTCCTCTCTG 
TTGCCCTCGC CTGGAGACCT GGGAGCTGAA CCAGCCACAT CTGGTCCTTC CCTGAGTGAG 
CAGGACTCTG TCAGCATCCC AGAAGATGTA TTTGATGGAC ACTTGCTGGG CTCCAATGAC 
AGTCAGGTGA AGGAGAAATC CACCATGAAA GCCATCCTTG CCAACTTCCT GCCCGGCAAC 
AGTTACAACC CCATTCCATT CCCATTTGAC CCAGACAAAC ACTACCTGAT GGATGAGTAT 
GAGCGAGTGC CAATTGCAGT GTGTGAAAGA GAACCAAGCT CCATCATTGC ATTTGCTCTG 
AGTTGTAAGA AGTATAAAAC AGCACTGGAA GATCTGTCCA AGGTGTCAAA CATGGGTGGA 
GAAGACTCTT CACAAGCTGC CAGTAGTGGA GAGAGTCGGG TGAGGGGCAG CCCCGCCAGG 
CCCAGTGAGT CAGCCTCGTC CCAACAGAGC CGCAGCAGCA GTGAAGCAGA TCCCCTCAAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_029832648.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029688508.1 Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X17, mRNA.

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CloneID	OTo40935
Clone ID Related Accession (Same CDS sequence)	XM_029832648.1
Accession Version	XM_029832648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGAGGAG GGACGTTCTC TTTCAACAGT GTCAGAGAAA 
TCAGACACTC CATCCCCTTC CCCTCTATCA GAGAGGAGGA GCTGGTCCTC CAGCCCACCC 
CATTCCCTCT ACACTGCCAG GATTCCCCGA AAACAACCAG ATCACCTTCA AAGGACCTCT 
GCTGCTTCAG ATGGCAGCAG GAAATCAGAG ACTCCTTTAA GCAATCACGA CCCTCGAACT 
GCTGTGCAAC TTCGCACTGC ATTGAAGAGG CTGAAGGAAA TAATGGAGGG AAAGAGCCAG 
GACAGTGACT TGAAGCAGTA CTGGATGCCG GACAGCCAGT GTAAAGAATG TTATGACTGC 
AATGAAAAGT TCACAACCTT CCGACGCCGC CACCATTGCC GACTGTGTGG TCAGATTTTC 
TGCAGTCGCT GCTGCAACCA GGAAATCCCT GGAAAGTTCA TGGGTTACAC AGGAGATTTG 
CGGGCCTGTA CGTACTGCCG TAAGTTGGCA CTGAGCTACG CCCACTCGGC TGATTCGGGC 
TCCATCGGCG AAGACTTGAA CACCCTGACT GACTCTCCGA GCTCTGTGTG TGTGCTGGAG 
CCCAGTGAGC CACGGACGCC TGTGGGGGGG CGCAAGGCCA GCAGGAACAT CTTCCTAGAG 
GAAGACTTAA CATGGCAGAG CATGATTCAC CAGGAACCTC AGAGCAGCGG CCTCAGTTCC 
AGACTGACAG GACTTCAGGA GGATGTAGGA AAATCACCTG GTAGGAGGAG GTCTGCTAGC 
ATCAGCACAC TTCCACTTGA CCGCTCTGGG TCATCAATGG TACCTTCCTA CGACAGCTCC 
GTCAGCCCCC CTACCAGCAG AGCCATGTCG GTGTCAAAGG CTGGGACAAA ATTAGATCAC 
AGTGAGGAGG AACGTAAAAT ACTACTGGAC TCCTCCCAGT TGAAGGACTT ATGGAGGAAA 
ATCTGTCATA ATGGAACAGG GATGGAATTC CAGGACCACA GATACTGGCT GAGAACCTAC 
CCCAACTGCA TTGTGGGAAA AGAGCTCGTC AACTGGCTGC TAAGAAATGG CACCATTTCC 
AACAGGGTCC AAGCTATTGC CATAGGTCAG GCACTCGTGG ATGGCCGCTG GCTCGACTGT 
GTCACCCACC ACGATCAGCT ATTCAGAGAT GAGTATGCTC TCTATCGACC ACTTCAGAGT 
ACAGAGTTCT CAGAAACTCC CTCCCCGGAC AGCGATAGTG TCAACTCACT AGAGGGACAT 
TCAGAGCCGT CATGGTTCAA GGACATCAAA TTCGATGACA GCGACACCGA GCAGCCCGCA 
GACGACACAG AGTACACCAT GCCTAGTTCC GCTGGGCCCA GCAAGAGGAC ATCTGTCAGC 
AGCGTCCACT CAGCAGTAGA CAGTGACTCA GCTGCCTCCA TCAACCTCAA CATGGAGCAA 
AACAATGTGA ATTTTCGAAT CAAGAAAGAT TCCAAGTGTC CCCAGGCATT CCCCGCCCTG 
GAGCACAAAT CTGAAATTAT GGCGTCTGAA GATGGAGGAG AGAACATCCT TATAAGTGAT 
GCCTTTATCA AAGAGTCTCT CTTTAACCGT CGTGTGGAGG AGAAGGCTAA AGAAATGCTG 
TTTACTCCAC TGGGTTGGCA CCACAGCTCT CTGGACCAGC TCAGAGAGGA GAATGGAGAA 
AAGAAGGCTA TGGAGAGGCT GCTCTCGGCA AATCACAGCC ACATGATGGC GCTGCTGCAG 
CAGTTGCTCT ACAGCGAGTC TTTGTCTCTG TCCTGGCGGG ATATTATCGT TCCTGTGGTT 
CGCCAGGTTG TCCAGACAGT TCGACCTGAT GTTCGCAACA GTGATGACGA CATGGACATC 
CGCCAACTGG TCCATATCAA AAAGATTCCT GGAGGAAAAA AGTTTGACTC AACTGTAGTG 
AATGGCTTTG TGTGTACGAA GAGTATTGCC CACAAAAAGA TGAACCCATA TATCAAGAAC 
CCCAAAATTC TGCTGCTGAA ATGCTCAATA GAGTATCTGT ACAGGGAAGA GACCAAATTC 
ACGTCCATCG ACCCCATCGT GCTGCAGGAA CGAGAGTTTC TGAAAAACTA TGTGCAACGT 
ATCGTGGATG TTCGGCCCAC TCTGGTTCTG GTGGAAAAAA CGGTGTCTCG TATAGCTCAG 
GACATGCTGC TAGAACACGG CATTACCCTG GTCATTAATG TCAAACCGCA AGTATTGGAC 
AGGGTGAGTT GTGTGACCCA GGGGGACCTT GTCATGTCCA TGGACCAGCT ACTCACCAAA 
CCTCGTCTGG GTACCTGCCA CAAGTTTTAC ATGCAGCCCT TCCCTTTGGC CAATAATGAG 
GTCAAGACTC TGATGTTCTT CGAAGGATGT CCTGCTCATC TCGGGTGCTC CATAAATCTA 
CGTGGTGCTT CAGACTACGA GTTAGCCAGG GTGAAGGAGA TCATGCGATT AATGGTGTGT 
GTGGCCTACC ACTCTCAGCT GGAGATCTCC TTCCTCATGG ATGAGTTTGC CATGCCGCCC 
AGCTTGTCCC AGAGCACTTC ATTTCCCTGT CTCCTCGACG GAGGCTCTGC AGATGAGGAA 
GCAAGCAGGG AGAAACTAGA GAAGAGTTCT GAAGATGAAG ACACTGTAGT GGAAGGACAA 
CCTGAGGAGG TGCCCTCTGC CAATGATGTA GACACTTCCC AGGACACAAA TGATCCTAAA 
TTATTCTCCT CTATGGTGGA AGAGACCACT AATACTGACA CAGTCACGAC AAAGTCTTCC 
ACTCCGTTCC AAAGCCCTCG TGCGCCATGC TTGCCAGTTC CTCCACCATT CATCATGGAA 
GAAACCCCAG AAATGGGCTC AGACAGCCTA ACAGGGGCAT TTGAGGCAGG GGTGGTGGAA 
TGCTGCCCAA AGGAGGAATC AACCAACCTA GAAGATGGAG AAGATTCTGA TATTCCTACT 
CCTAAGTTGT TCCGAGACCC CCTGCAAGAT GATACTGGGA TGTTTGTGGC TGAAGAAGTT 
GCTTCTTCAG ATGACCATCT CAAGTCCATC TCTGCTATCT TCAAACAGGA ACTTAAGGAC 
ATCATCCTGT GCATTTCACC TTTCATCACA TTTGAGAAAC CATTTCTTCT TACCCCTGCT 
GGGATGCACT GCCCGAGCAG GGATTATTTT CCCGAACAGG TCTATCTGTC TCCTCTCCTC 
AACAAGGACT TTAAAGAGTT GGATGGACGC AGAAAGCGGC AGCTCCTTAA GGAGTCCACT 
CCCTCCCTGG CAAACAGCCT AACGAATGGT GGCCCCCCGT CAAAGCATGT CAGTGTCCTG 
CCATCCCACA GTCTCACCAG CACCCGGATC GTTGAACTAG TAAACAGGAG CCAGGGTCTG 
GCCAGAATGG TTGCAGACTA CAGAGCAAAG GGTGGACGTA TCCAGCACAG CGAAGCTACT 
GATCCATTTT GCATAAGCAG CTCTTCTGCT CCGGTCAGTG GCCAGAATAG ACCAGGTGAT 
GGTTCGGTCA GAGCACCAGT AAAGACTGAC AGCGAAGAGG AGAAGCCAAC TAAACCCAGT 
GACTGGACCC CCAAGCTGGA TTGTCTGAAC CCCACCAACC ACCAGAGACT CTGTGTGCTT 
TTTAGCAGTT CATCAACTCA GTCCAGCAAT GCCCCCAACC CCTGTGTCAA CCCATGGATT 
GTTACCATGG AGTTTTATGG CAAGAATGAC CTCTGCCTTG GTGTATTCTT GGAGCGATAT 
TGCTTTAGGC CATCATACCA GTGCCCCAGT ATATTTTGTG AGACCCCCAT GGTGCACCAC 
ATCCGGCGTT TTGTCCATGG CAACGGCTGC GTGCAGATAG TACTGAAGGA GCTGGACTCC 
CCTGTGCCGG GGTACCAGCA CACCATTCTC AACTACTCCT GGTGCCGCAT CTGCAAACAA 
GTGACACCAG TGGTTCCTCT ATCCAATGAC TCATGGTCAA TGTCTTTTGC AAAATATCTG 
GAGCTTCGCT TCTACGGCCA CCAGTACACC AGGAGGGCCA ATGCAGAGCC CTGTGGCCAC 
TCCATCCACA AAGACTACCA CCAGTACTTC TCATACAACC AAATGGTGGC TTCCTTCAGT 
TACACCTCAG TGAGGCTGCT GGAGATTTGT CTTCCTCGTT TCAAAATCTT CATCAGGAAT 
CAGGGTCCTT CGATCAACAA CTTGGGGCAG GATCTGAAAG ACTTCTCTCA AAAAGTAAAT 
CAGGTTTACG TGGCCATAGA TGATCGTCTC ACATCTCTAA AGACGGACAC CTTCAGTAAA 
ACACGCGAGG AGAAGATGGA GGACCTCTTT GCACAGAAAG ATATGGAAGA GGCGGAACTG 
CGCAGCTGGA TTGAAAAGTT TCAAGCACGA TTGCAGGCTT GTGGTCACGT TTCTCCTCAG 
CAGCTGCAGA CGACTCTGGA ATCACTGGTG ATGAAGAAAC AGAGTCTGTG TGAGATGTTG 
CAGTACTGGA ACAGCAGGCT GCAGGAATTA TTCCAACAGG AGAAAGGCAG GAAACGTCTG 
TCAGTGCCCC CAAGCCCCGG CAGGCACAGA CAGACTGCCC CTGATGATGG CAAGAACGCT 
GTGGAGTCAT CCCCACGTAA CCCTTCACCT GTGGTACAGA ATGGTGAAAA GGAGGACCGT 
CACCTCACCA CACTGCCTTC AACCTCCTCT CTGTTGCCCT CGCCTGGAGA CCTGGGAGCT 
GAACCAGCCA CATCTGGTCC TTCCCTGAGT GAGCAGGACT CTGTCAGCAT CCCAGAAGAT 
GTATTTGATG GACACTTGCT GGGCTCCAAT GACAGTCAGG TGAAGGAGAA ATCCACCATG 
AAAGCCATCC TTGCCAACTT CCTGCCCGGC AACAGTTACA ACCCCATTCC ATTCCCATTT 
GACCCAGACA AACACTACCT GATGGATGAG TATGAGCGAG TGCCAATTGC AGTGTGTGAA 
AGAGAACCAA GCTCCATCAT TGCATTTGCT CTGAGTTGTA AGAAGTATAA AACAGCACTG 
GAAGATCTGT CCAAGGTGTC AAACATGGGT GGAGAAGACT CTTCACAAGC TGCCAGTAGT 
GGAGAGAGTC GGGTGAGGGG CAGCCCCGCC AGGCCCAGTG AGTCAGCCTC GTCCCAACAG 
AGCCGCAGCA GCAGTGAAGC AGATCCCCTC AAAGACGCAG ACCTAACAGA CAAACAGAAG 
AAGCAGGCCC TGAATCCCCA CATTGAGCTA CAGTTCTCTG ATGCAAGCGC AAAGTTTTAC 
TGTCGGATCT ACTACGCCGA GGAGTTTCAT AAGATGCGTG AGGAGATCAT GGAGAGCTCC 
GAAGAGGACT TTGTGCGTTC CCTGTCGCAC TGTGTCAACT GGCAGGCGCG CGGTGGAAAG 
TCTGGGGCCG TTTTCTACGC AACAGAAGAC GACCGTTTCA TCCTAAAACA GATGCCCAGG 
CTGGAGGTGC AGTCCTTCTT GGACTTTGCT CCGCACTACT TCACCTACAT CACCGGAGCT 
GTGCAGCAGA AGAGGCCAAC TGCATTGGCA AAGATTCTCG GAGTTTACAG GATAGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAA CTGGACCTGC TCGTCATGGA AAACCTGTTC 
TACGGACGCA AGATGGCTCA GGTCTTTGAC CTGAAAGGTT CCCTCAGAAA CCGTCATGTG 
AAGACAGACC TGGTAAAGGA AAGCTGCGAG GTGGTCCTGC TGGATGAGAA TCTACTGAAG 
CTGATCCACG ACAATCCGCT CTACATTCGC TCTCACTGCA AGTCCATCTT GCGCGCCGCC 
ATACACAGCG ACGCCTACTT CCTGTCCAGC CACCTCATCA TTGACTACTC CCTGCTGGTG 
GGGTGGGACG ATGCCACCGA CCAGCTGGTG GTTGGGATCA TAGACTATAT TCGGACGTTT 
ACCTGGGACA AGAAATTAGA GATGGTTGTC AAATCCACTG GAATCCTGGG GGGTCAGGGA 
AAGATGCCCA CCGTTGTTTC TCCTGAGCTG TACCGAGCTC GGTTCTGTGA GGCCATGGAC 
AAATACTTCC TGATGGTACC CGACCACTGG ACGGGTCTTG GCATCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029688508.1
CDS	128..6361
Translation	MAHRTSSVMAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKEEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQSMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_029832648.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE-type zinc finger containing (pikfyve), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029832648.1

ORF Insert Sequence:

ATGGCTCACA GGACCAGCAG TGTCATGGCT GCTGATGATA AGTCATCCTC ATCCTCAATG 
GAGTGGACCA CTGAGACCCC CGTCACCTTG CCCGTGAGTC CGTCCCACCT GACTCATTTT 
AAGCCACTGT CACCCGAGCA GGATGAGCCC CCGCTCCGTT CAGCATACAG CTCATTTGTC 
AACCTATTTC GCTTTAGTAA CAAAGAGGAG GGACGTTCTC TTTCAACAGT GTCAGAGAAA 
TCAGACACTC CATCCCCTTC CCCTCTATCA GAGAGGAGGA GCTGGTCCTC CAGCCCACCC 
CATTCCCTCT ACACTGCCAG GATTCCCCGA AAACAACCAG ATCACCTTCA AAGGACCTCT 
GCTGCTTCAG ATGGCAGCAG GAAATCAGAG ACTCCTTTAA GCAATCACGA CCCTCGAACT 
GCTGTGCAAC TTCGCACTGC ATTGAAGAGG CTGAAGGAAA TAATGGAGGG AAAGAGCCAG 
GACAGTGACT TGAAGCAGTA CTGGATGCCG GACAGCCAGT GTAAAGAATG TTATGACTGC 
AATGAAAAGT TCACAACCTT CCGACGCCGC CACCATTGCC GACTGTGTGG TCAGATTTTC 
TGCAGTCGCT GCTGCAACCA GGAAATCCCT GGAAAGTTCA TGGGTTACAC AGGAGATTTG 
CGGGCCTGTA CGTACTGCCG TAAGTTGGCA CTGAGCTACG CCCACTCGGC TGATTCGGGC 
TCCATCGGCG AAGACTTGAA CACCCTGACT GACTCTCCGA GCTCTGTGTG TGTGCTGGAG 
CCCAGTGAGC CACGGACGCC TGTGGGGGGG CGCAAGGCCA GCAGGAACAT CTTCCTAGAG 
GAAGACTTAA CATGGCAGAG CATGATTCAC CAGGAACCTC AGAGCAGCGG CCTCAGTTCC 
AGACTGACAG GACTTCAGGA GGATGTAGGA AAATCACCTG GTAGGAGGAG GTCTGCTAGC 
ATCAGCACAC TTCCACTTGA CCGCTCTGGG TCATCAATGG TACCTTCCTA CGACAGCTCC 
GTCAGCCCCC CTACCAGCAG AGCCATGTCG GTGTCAAAGG CTGGGACAAA ATTAGATCAC 
AGTGAGGAGG AACGTAAAAT ACTACTGGAC TCCTCCCAGT TGAAGGACTT ATGGAGGAAA 
ATCTGTCATA ATGGAACAGG GATGGAATTC CAGGACCACA GATACTGGCT GAGAACCTAC 
CCCAACTGCA TTGTGGGAAA AGAGCTCGTC AACTGGCTGC TAAGAAATGG CACCATTTCC 
AACAGGGTCC AAGCTATTGC CATAGGTCAG GCACTCGTGG ATGGCCGCTG GCTCGACTGT 
GTCACCCACC ACGATCAGCT ATTCAGAGAT GAGTATGCTC TCTATCGACC ACTTCAGAGT 
ACAGAGTTCT CAGAAACTCC CTCCCCGGAC AGCGATAGTG TCAACTCACT AGAGGGACAT 
TCAGAGCCGT CATGGTTCAA GGACATCAAA TTCGATGACA GCGACACCGA GCAGCCCGCA 
GACGACACAG AGTACACCAT GCCTAGTTCC GCTGGGCCCA GCAAGAGGAC ATCTGTCAGC 
AGCGTCCACT CAGCAGTAGA CAGTGACTCA GCTGCCTCCA TCAACCTCAA CATGGAGCAA 
AACAATGTGA ATTTTCGAAT CAAGAAAGAT TCCAAGTGTC CCCAGGCATT CCCCGCCCTG 
GAGCACAAAT CTGAAATTAT GGCGTCTGAA GATGGAGGAG AGAACATCCT TATAAGTGAT 
GCCTTTATCA AAGAGTCTCT CTTTAACCGT CGTGTGGAGG AGAAGGCTAA AGAAATGCTG 
TTTACTCCAC TGGGTTGGCA CCACAGCTCT CTGGACCAGC TCAGAGAGGA GAATGGAGAA 
AAGAAGGCTA TGGAGAGGCT GCTCTCGGCA AATCACAGCC ACATGATGGC GCTGCTGCAG 
CAGTTGCTCT ACAGCGAGTC TTTGTCTCTG TCCTGGCGGG ATATTATCGT TCCTGTGGTT 
CGCCAGGTTG TCCAGACAGT TCGACCTGAT GTTCGCAACA GTGATGACGA CATGGACATC 
CGCCAACTGG TCCATATCAA AAAGATTCCT GGAGGAAAAA AGTTTGACTC AACTGTAGTG 
AATGGCTTTG TGTGTACGAA GAGTATTGCC CACAAAAAGA TGAACCCATA TATCAAGAAC 
CCCAAAATTC TGCTGCTGAA ATGCTCAATA GAGTATCTGT ACAGGGAAGA GACCAAATTC 
ACGTCCATCG ACCCCATCGT GCTGCAGGAA CGAGAGTTTC TGAAAAACTA TGTGCAACGT 
ATCGTGGATG TTCGGCCCAC TCTGGTTCTG GTGGAAAAAA CGGTGTCTCG TATAGCTCAG 
GACATGCTGC TAGAACACGG CATTACCCTG GTCATTAATG TCAAACCGCA AGTATTGGAC 
AGGGTGAGTT GTGTGACCCA GGGGGACCTT GTCATGTCCA TGGACCAGCT ACTCACCAAA 
CCTCGTCTGG GTACCTGCCA CAAGTTTTAC ATGCAGCCCT TCCCTTTGGC CAATAATGAG 
GTCAAGACTC TGATGTTCTT CGAAGGATGT CCTGCTCATC TCGGGTGCTC CATAAATCTA 
CGTGGTGCTT CAGACTACGA GTTAGCCAGG GTGAAGGAGA TCATGCGATT AATGGTGTGT 
GTGGCCTACC ACTCTCAGCT GGAGATCTCC TTCCTCATGG ATGAGTTTGC CATGCCGCCC 
AGCTTGTCCC AGAGCACTTC ATTTCCCTGT CTCCTCGACG GAGGCTCTGC AGATGAGGAA 
GCAAGCAGGG AGAAACTAGA GAAGAGTTCT GAAGATGAAG ACACTGTAGT GGAAGGACAA 
CCTGAGGAGG TGCCCTCTGC CAATGATGTA GACACTTCCC AGGACACAAA TGATCCTAAA 
TTATTCTCCT CTATGGTGGA AGAGACCACT AATACTGACA CAGTCACGAC AAAGTCTTCC 
ACTCCGTTCC AAAGCCCTCG TGCGCCATGC TTGCCAGTTC CTCCACCATT CATCATGGAA 
GAAACCCCAG AAATGGGCTC AGACAGCCTA ACAGGGGCAT TTGAGGCAGG GGTGGTGGAA 
TGCTGCCCAA AGGAGGAATC AACCAACCTA GAAGATGGAG AAGATTCTGA TATTCCTACT 
CCTAAGTTGT TCCGAGACCC CCTGCAAGAT GATACTGGGA TGTTTGTGGC TGAAGAAGTT 
GCTTCTTCAG ATGACCATCT CAAGTCCATC TCTGCTATCT TCAAACAGGA ACTTAAGGAC 
ATCATCCTGT GCATTTCACC TTTCATCACA TTTGAGAAAC CATTTCTTCT TACCCCTGCT 
GGGATGCACT GCCCGAGCAG GGATTATTTT CCCGAACAGG TCTATCTGTC TCCTCTCCTC 
AACAAGGACT TTAAAGAGTT GGATGGACGC AGAAAGCGGC AGCTCCTTAA GGAGTCCACT 
CCCTCCCTGG CAAACAGCCT AACGAATGGT GGCCCCCCGT CAAAGCATGT CAGTGTCCTG 
CCATCCCACA GTCTCACCAG CACCCGGATC GTTGAACTAG TAAACAGGAG CCAGGGTCTG 
GCCAGAATGG TTGCAGACTA CAGAGCAAAG GGTGGACGTA TCCAGCACAG CGAAGCTACT 
GATCCATTTT GCATAAGCAG CTCTTCTGCT CCGGTCAGTG GCCAGAATAG ACCAGGTGAT 
GGTTCGGTCA GAGCACCAGT AAAGACTGAC AGCGAAGAGG AGAAGCCAAC TAAACCCAGT 
GACTGGACCC CCAAGCTGGA TTGTCTGAAC CCCACCAACC ACCAGAGACT CTGTGTGCTT 
TTTAGCAGTT CATCAACTCA GTCCAGCAAT GCCCCCAACC CCTGTGTCAA CCCATGGATT 
GTTACCATGG AGTTTTATGG CAAGAATGAC CTCTGCCTTG GTGTATTCTT GGAGCGATAT 
TGCTTTAGGC CATCATACCA GTGCCCCAGT ATATTTTGTG AGACCCCCAT GGTGCACCAC 
ATCCGGCGTT TTGTCCATGG CAACGGCTGC GTGCAGATAG TACTGAAGGA GCTGGACTCC 
CCTGTGCCGG GGTACCAGCA CACCATTCTC AACTACTCCT GGTGCCGCAT CTGCAAACAA 
GTGACACCAG TGGTTCCTCT ATCCAATGAC TCATGGTCAA TGTCTTTTGC AAAATATCTG 
GAGCTTCGCT TCTACGGCCA CCAGTACACC AGGAGGGCCA ATGCAGAGCC CTGTGGCCAC 
TCCATCCACA AAGACTACCA CCAGTACTTC TCATACAACC AAATGGTGGC TTCCTTCAGT 
TACACCTCAG TGAGGCTGCT GGAGATTTGT CTTCCTCGTT TCAAAATCTT CATCAGGAAT 
CAGGGTCCTT CGATCAACAA CTTGGGGCAG GATCTGAAAG ACTTCTCTCA AAAAGTAAAT 
CAGGTTTACG TGGCCATAGA TGATCGTCTC ACATCTCTAA AGACGGACAC CTTCAGTAAA 
ACACGCGAGG AGAAGATGGA GGACCTCTTT GCACAGAAAG ATATGGAAGA GGCGGAACTG 
CGCAGCTGGA TTGAAAAGTT TCAAGCACGA TTGCAGGCTT GTGGTCACGT TTCTCCTCAG 
CAGCTGCAGA CGACTCTGGA ATCACTGGTG ATGAAGAAAC AGAGTCTGTG TGAGATGTTG 
CAGTACTGGA ACAGCAGGCT GCAGGAATTA TTCCAACAGG AGAAAGGCAG GAAACGTCTG 
TCAGTGCCCC CAAGCCCCGG CAGGCACAGA CAGACTGCCC CTGATGATGG CAAGAACGCT 
GTGGAGTCAT CCCCACGTAA CCCTTCACCT GTGGTACAGA ATGGTGAAAA GGAGGACCGT 
CACCTCACCA CACTGCCTTC AACCTCCTCT CTGTTGCCCT CGCCTGGAGA CCTGGGAGCT 
GAACCAGCCA CATCTGGTCC TTCCCTGAGT GAGCAGGACT CTGTCAGCAT CCCAGAAGAT 
GTATTTGATG GACACTTGCT GGGCTCCAAT GACAGTCAGG TGAAGGAGAA ATCCACCATG 
AAAGCCATCC TTGCCAACTT CCTGCCCGGC AACAGTTACA ACCCCATTCC ATTCCCATTT 
GACCCAGACA AACACTACCT GATGGATGAG TATGAGCGAG TGCCAATTGC AGTGTGTGAA 
AGAGAACCAA GCTCCATCAT TGCATTTGCT CTGAGTTGTA AGAAGTATAA AACAGCACTG 
GAAGATCTGT CCAAGGTGTC AAACATGGGT GGAGAAGACT CTTCACAAGC TGCCAGTAGT 
GGAGAGAGTC GGGTGAGGGG CAGCCCCGCC AGGCCCAGTG AGTCAGCCTC GTCCCAACAG 
AGCCGCAGCA GCAGTGAAGC AGATCCCCTC AAAGACGCAG ACCTAACAGA CAAACAGAAG 
AAGCAGGCCC TGAATCCCCA CATTGAGCTA CAGTTCTCTG ATGCAAGCGC AAAGTTTTAC 
TGTCGGATCT ACTACGCCGA GGAGTTTCAT AAGATGCGTG AGGAGATCAT GGAGAGCTCC 
GAAGAGGACT TTGTGCGTTC CCTGTCGCAC TGTGTCAACT GGCAGGCGCG CGGTGGAAAG 
TCTGGGGCCG TTTTCTACGC AACAGAAGAC GACCGTTTCA TCCTAAAACA GATGCCCAGG 
CTGGAGGTGC AGTCCTTCTT GGACTTTGCT CCGCACTACT TCACCTACAT CACCGGAGCT 
GTGCAGCAGA AGAGGCCAAC TGCATTGGCA AAGATTCTCG GAGTTTACAG GATAGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAA CTGGACCTGC TCGTCATGGA AAACCTGTTC 
TACGGACGCA AGATGGCTCA GGTCTTTGAC CTGAAAGGTT CCCTCAGAAA CCGTCATGTG 
AAGACAGACC TGGTAAAGGA AAGCTGCGAG GTGGTCCTGC TGGATGAGAA TCTACTGAAG 
CTGATCCACG ACAATCCGCT CTACATTCGC TCTCACTGCA AGTCCATCTT GCGCGCCGCC 
ATACACAGCG ACGCCTACTT CCTGTCCAGC CACCTCATCA TTGACTACTC CCTGCTGGTG 
GGGTGGGACG ATGCCACCGA CCAGCTGGTG GTTGGGATCA TAGACTATAT TCGGACGTTT 
ACCTGGGACA AGAAATTAGA GATGGTTGTC AAATCCACTG GAATCCTGGG GGGTCAGGGA 
AAGATGCCCA CCGTTGTTTC TCCTGAGCTG TACCGAGCTC GGTTCTGTGA GGCCATGGAC 
AAATACTTCC TGATGGTACC CGACCACTGG ACGGGTCTTG GCATCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_003961635.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003961684.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.

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CloneID	OTo00680
Clone ID Related Accession (Same CDS sequence)	XM_003961635.2
Accession Version	XM_003961635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003961635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GAGGAGGGAC GTTCTCTTTC AACAGTGTCA GAGAAATCAG ACACTCCATC CCCTTCCCCT 
CTATCAGAGA GGAGGAGCTG GTCCTCCAGC CCACCCCATT CCCTCTACAC TGCCAGGATT 
CCCCGAAAAC AACCAGATCA CCTTCAAAGG ACCTCTGCTG CTTCAGATGG CAGCAGGAAA 
TCAGAGACTC CTTTAAGCAA TCACGACCCT CGAACTGCTG TGCAACTTCG CACTGCATTG 
AAGAGGCTGA AGGAAATAAT GGAGGGAAAG AGCCAGGACA GTGACTTGAA GCAGTACTGG 
ATGCCGGACA GCCAGTGTAA AGAATGTTAT GACTGCAATG AAAAGTTCAC AACCTTCCGA 
CGCCGCCACC ATTGCCGACT GTGTGGTCAG ATTTTCTGCA GTCGCTGCTG CAACCAGGAA 
ATCCCTGGAA AGTTCATGGG TTACACAGGA GATTTGCGGG CCTGTACGTA CTGCCGTAAG 
TTGGCACTGA GCTACGCCCA CTCGGCTGAT TCGGGCTCCA TCGGCGAAGA CTTGAACACC 
CTGACTGACT CTCCGAGCTC TGTGTGTGTG CTGGAGCCCA GTGAGCCACG GACGCCTGTG 
GGGGGGCGCA AGGCCAGCAG GAACATCTTC CTAGAGGAAG ACTTAACATG GCAGAGCATG 
ATTCACCAGG AACCTCAGAG CAGCGGCCTC AGTTCCAGAC TGACAGGACT TCAGGAGGAT 
GTAGGAAAAT CACCTGGTAG GAGGAGGTCT GCTAGCATCA GCACACTTCC ACTTGACCGC 
TCTGGGTCAT CAATGGTACC TTCCTACGAC AGCTCCGTCA GCCCCCCTAC CAGCAGAGCC 
ATGTCGGTGT CAAAGGCTGG GACAAAATTA GATCACAGTG AGGAGGAACG TAAAATACTA 
CTGGACTCCT CCCAGTTGAA GGACTTATGG AGGAAAATCT GTCATAATGG AACAGGGATG 
GAATTCCAGG ACCACAGATA CTGGCTGAGA ACCTACCCCA ACTGCATTGT GGGAAAAGAG 
CTCGTCAACT GGCTGCTAAG AAATGGCACC ATTTCCAACA GGGTCCAAGC TATTGCCATA 
GGTCAGGCAC TCGTGGATGG CCGCTGGCTC GACTGTGTCA CCCACCACGA TCAGCTATTC 
AGAGATGAGT ATGCTCTCTA TCGACCACTT CAGAGTACAG AGTTCTCAGA AACTCCCTCC 
CCGGACAGCG ATAGTGTCAA CTCACTAGAG GGACATTCAG AGCCGTCATG GTTCAAGGAC 
ATCAAATTCG ATGACAGCGA CACCGAGCAG CCCGCAGACG ACACAGAGTA CACCATGCCT 
AGTTCCGCTG GGCCCAGCAA GAGGACATCT GTCAGCAGCG TCCACTCAGC AGTAGACAGT 
GACTCAGCTG CCTCCATCAA CCTCAACATG GAGCAAAACA ATGTGAATTT TCGAATCAAG 
AAAGATTCCA AGTGTCCCCA GGCATTCCCC GCCCTGGAGC ACAAATCTGA AATTATGGCG 
TCTGAAGATG GAGGAGAGAA CATCCTTATA AGTGATGCCT TTATCAAAGA GTCTCTCTTT 
AACCGTCGTG TGGAGGAGAA GGCTAAAGAA ATGCTGTTTA CTCCACTGGG TTGGCACCAC 
AGCTCTCTGG ACCAGCTCAG AGAGGAGAAT GGAGAAAAGA AGGCTATGGA GAGGCTGCTC 
TCGGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAGT TGCTCTACAG CGAGTCTTTG 
TCTCTGTCCT GGCGGGATAT TATCGTTCCT GTGGTTCGCC AGGTTGTCCA GACAGTTCGA 
CCTGATGTTC GCAACAGTGA TGACGACATG GACATCCGCC AACTGGTCCA TATCAAAAAG 
ATTCCTGGAG GAAAAAAGTT TGACTCAACT GTAGTGAATG GCTTTGTGTG TACGAAGAGT 
ATTGCCCACA AAAAGATGAA CCCATATATC AAGAACCCCA AAATTCTGCT GCTGAAATGC 
TCAATAGAGT ATCTGTACAG GGAAGAGACC AAATTCACGT CCATCGACCC CATCGTGCTG 
CAGGAACGAG AGTTTCTGAA AAACTATGTG CAACGTATCG TGGATGTTCG GCCCACTCTG 
GTTCTGGTGG AAAAAACGGT GTCTCGTATA GCTCAGGACA TGCTGCTAGA ACACGGCATT 
ACCCTGGTCA TTAATGTCAA ACCGCAAGTA TTGGACAGGG TGAGTTGTGT GACCCAGGGG 
GACCTTGTCA TGTCCATGGA CCAGCTACTC ACCAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACATGC AGCCCTTCCC TTTGGCCAAT AATGAGGTCA AGACTCTGAT GTTCTTCGAA 
GGATGTCCTG CTCATCTCGG GTGCTCCATA AATCTACGTG GTGCTTCAGA CTACGAGTTA 
GCCAGGGTGA AGGAGATCAT GCGATTAATG GTGTGTGTGG CCTACCACTC TCAGCTGGAG 
ATCTCCTTCC TCATGGATGA GTTTGCCATG CCGCCCAGCT TGTCCCAGAG CACTTCATTT 
CCCTGTCTCC TCGACGGAGG CTCTGCAGAT GAGGAAGCAA GCAGGGAGAA ACTAGAGAAG 
AGTTCTGAAG ATGAAGACAC TGTAGTGGAA GGACAACCTG AGGAGGTGCC CTCTGCCAAT 
GATGTAGACA CTTCCCAGGA CACAAATGAT CCTAAATTAT TCTCCTCTAT GGTGGAAGAG 
ACCACTAATA CTGACACAGT CACGACAAAG TCTTCCACTC CGTTCCAAAG CCCTCGTGCG 
CCATGCTTGC CAGTTCCTCC ACCATTCATC ATGGAAGAAA CCCCAGAAAT GGGCTCAGAC 
AGCCTAACAG GGGCATTTGA GGCAGGGGTG GTGGAATGCT GCCCAAAGGA GGAATCAACC 
AACCTAGAAG ATGGAGAAGA TTCTGATATT CCTACTCCTA AATTGTTCCG AGACCCCCTG 
CAAGATGATA CTGGGATGTT TGTGGCTGAA GAAGTTGCTT CTTCAGATGA CCATCTCAAG 
TCCATCTCTG CTATCTTCAA ACAGGAACTT AAGGACATCA TCCTGTGCAT TTCACCTTTC 
ATCACATTTG AGAAACCATT TCTTCTTACC CCTGCTGGGA TGCACTGCCC GAGCAGGGAT 
TATTTTCCCG AACAGGTCTA TCTGTCTCCT CTCCTCAACA AGGACTTTAA AGAGTTGGAT 
GGACGCAGAA AGCGGCAGCT CCTTAAGGAG TCCACTCCCT CCCTGGCAAA CAGCCTAACG 
AATGGTGGCC CCCCGTCAAA GCATGTCAGT GTCCTGCCAT CCCACAGTCT CACCAGCACC 
CGGATCGTTG AACTAGTAAA CAGGAGCCAG GGTCTGGCCA GAATGGTTGC AGACTACAGA 
GCAAAGGGTG GACGTATCCA GCACAGCGAA GCTACTGATC CATTTTGCAT AAGCAGCTCT 
TCTGCTCCGG TCAGTGGCCA GAATAGACCA GGTGATGGTT CGGTCAGAGC ACCAGTAAAG 
ACTGACAGCG AAGAGGAGAA GCCAACTAAA CCCAGTGACT GGACCCCCAA GCTGGATTGT 
CTGAACCCCA CCAACCACCA GAGACTCTGT GTGCTTTTTA GCAGTTCATC AACTCAGTCC 
AGCAATGCCC CCAACCCCTG TGTCAACCCA TGGATTGTTA CCATGGAGTT TTATGGCAAG 
AATGACCTCT GCCTTGGTGT ATTCTTGGAG CGATATTGCT TTAGGCCATC ATACCAGTGC 
CCCAGTATAT TTTGTGAGAC CCCCATGGTG CACCACATCC GGCGTTTTGT CCATGGCAAC 
GGCTGCGTGC AGATAGTACT GAAGGAGCTG GACTCCCCTG TGCCGGGGTA CCAGCACACC 
ATTCTCAACT ACTCCTGGTG CCGCATCTGC AAACAAGTGA CACCAGTGGT TCCTCTATCC 
AATGACTCAT GGTCAATGTC TTTTGCAAAA TATCTGGAGC TTCGCTTCTA CGGCCACCAG 
TACACCAGGA GGGCCAATGC AGAGCCCTGT GGCCACTCCA TCCACAAAGA CTACCACCAG 
TACTTCTCAT ACAACCAAAT GGTGGCTTCC TTCAGTTACA CCTCAGTGAG GCTGCTGGAG 
ATTTGTCTTC CTCGTTTCAA AATCTTCATC AGGAATCAGG GTCCTTCGAT CAACAACTTG 
GGGCAGGATC TGAAAGACTT CTCTCAAAAA GTAAATCAGG TTTACGTGGC CATAGATGAT 
CGTCTCACAT CTCTAAAGAC GGACACCTTC AGTAAAACAC GCGAGGAGAA GATGGAGGAC 
CTCTTTGCAC AGAAAGATAT GGAAGAGGCG GAACTGCGCA GCTGGATTGA AAAGTTTCAA 
GCACGATTGC AGGCTTGTGG TCACGTTTCT CCTCAGCAGC TGCAGACGAC TCTGGAATCA 
CTGGTGATGA AGAAACAGAG TCTGTGTGAG ATGTTGCAGT ACTGGAACAG CAGGCTGCAG 
GAATTATTCC AACAGGAGAA AGGCAGGAAA CGTCTGTCAG TGCCCCCAAG CCCCGGCAGG 
CACAGACAGA CTGCCCCTGA TGATGGCAAG AACGCTGTGG AGTCATCCCC ACGTAACCCT 
TCACCTGTGG TACAGAATGG TGAAAAGGAG GACCGTCACC TCACCACACT GCCTTCAACC 
TCCTCTCTGT TGCCCTCGCC TGGAGACCTG GGAGCTGAAC CAGCCACATC TGGTCCTTCC 
CTGAGTGAGC AGGACTCTGT CAGCATCCCA GAAGATGTAT TTGATGGACA CTTGCTGGGC 
TCCAATGACA GTCAGGTGAA GGAGAAATCC ACCATGAAAG CCATCCTTGC CAACTTCCTG 
CCCGGCAACA GTTACAACCC CATTCCATTC CCATTTGACC CAGACAAACA CTACCTGATG 
GATGAGTATG AGCGAGTGCC AATTGCAGTG TGTGAAAGAG AACCAAGCTC CATCATTGCA 
TTTGCTCTGA GTTGTAAGAA GTATAAAACA GCACTGGAAG ATCTGTCCAA GGTGTCAAAC 
ATGGGTGGAG AAGACTCTTC ACAAGCTGCC AGTAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAAAG ACGCAGACCT AACAGACAAA CAGAAGAAGC AGGCCCTGAA TCCCCACATT 
GAGCTACAGT TCTCTGATGC AAGCGCAAAG TTTTACTGTC GGATCTACTA CGCCGAGGAG 
TTTCATAAGA TGCGTGAGGA GATCATGGAG AGCTCCGAAG AGGACTTTGT GCGTTCCCTG 
TCGCACTGTG TCAACTGGCA GGCGCGCGGT GGAAAGTCTG GGGCCGTTTT CTACGCAACA 
GAAGACGACC GTTTCATCCT AAAACAGATG CCCAGGCTGG AGGTGCAGTC CTTCTTGGAC 
TTTGCTCCGC ACTACTTCAC CTACATCACC GGAGCTGTGC AGCAGAAGAG GCCAACTGCA 
TTGGCAAAGA TTCTCGGAGT TTACAGGATA GGCTACAAGA ACTCCCAGAA CAACACTGAG 
AAGAAACTGG ACCTGCTCGT CATGGAAAAC CTGTTCTACG GACGCAAGAT GGCTCAGGTC 
TTTGACCTGA AAGGTTCCCT CAGAAACCGT CATGTGAAGA CAGACCTGGT AAAGGAAAGC 
TGCGAGGTGG TCCTGCTGGA TGAGAATCTA CTGAAGCTGA TCCACGACAA TCCGCTCTAC 
ATTCGCTCTC ACTGCAAGTC CATCTTGCGC GCCGCCATAC ACAGCGACGC CTACTTCCTG 
TCCAGCCACC TCATCATTGA CTACTCCCTG CTGGTGGGGT GGGACGATGC CACCGACCAG 
CTGGTGGTTG GGATCATAGA CTATATTCGG ACGTTTACCT GGGACAAGAA ATTAGAGATG 
GTTGTCAAAT CCACTGGAAT CCTGGGGGGT CAGGGAAAGA TGCCCACCGT TGTTTCTCCT 
GAGCTGTACC GAGCTCGGTT CTGTGAGGCC ATGGACAAAT ACTTCCTGAT GGTACCCGAC 
CACTGGACGG GTCTTGGCAT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961684.1
CDS	123..6332
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKEEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQSMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_003961635.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961635.2

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GAGGAGGGAC GTTCTCTTTC AACAGTGTCA GAGAAATCAG ACACTCCATC CCCTTCCCCT 
CTATCAGAGA GGAGGAGCTG GTCCTCCAGC CCACCCCATT CCCTCTACAC TGCCAGGATT 
CCCCGAAAAC AACCAGATCA CCTTCAAAGG ACCTCTGCTG CTTCAGATGG CAGCAGGAAA 
TCAGAGACTC CTTTAAGCAA TCACGACCCT CGAACTGCTG TGCAACTTCG CACTGCATTG 
AAGAGGCTGA AGGAAATAAT GGAGGGAAAG AGCCAGGACA GTGACTTGAA GCAGTACTGG 
ATGCCGGACA GCCAGTGTAA AGAATGTTAT GACTGCAATG AAAAGTTCAC AACCTTCCGA 
CGCCGCCACC ATTGCCGACT GTGTGGTCAG ATTTTCTGCA GTCGCTGCTG CAACCAGGAA 
ATCCCTGGAA AGTTCATGGG TTACACAGGA GATTTGCGGG CCTGTACGTA CTGCCGTAAG 
TTGGCACTGA GCTACGCCCA CTCGGCTGAT TCGGGCTCCA TCGGCGAAGA CTTGAACACC 
CTGACTGACT CTCCGAGCTC TGTGTGTGTG CTGGAGCCCA GTGAGCCACG GACGCCTGTG 
GGGGGGCGCA AGGCCAGCAG GAACATCTTC CTAGAGGAAG ACTTAACATG GCAGAGCATG 
ATTCACCAGG AACCTCAGAG CAGCGGCCTC AGTTCCAGAC TGACAGGACT TCAGGAGGAT 
GTAGGAAAAT CACCTGGTAG GAGGAGGTCT GCTAGCATCA GCACACTTCC ACTTGACCGC 
TCTGGGTCAT CAATGGTACC TTCCTACGAC AGCTCCGTCA GCCCCCCTAC CAGCAGAGCC 
ATGTCGGTGT CAAAGGCTGG GACAAAATTA GATCACAGTG AGGAGGAACG TAAAATACTA 
CTGGACTCCT CCCAGTTGAA GGACTTATGG AGGAAAATCT GTCATAATGG AACAGGGATG 
GAATTCCAGG ACCACAGATA CTGGCTGAGA ACCTACCCCA ACTGCATTGT GGGAAAAGAG 
CTCGTCAACT GGCTGCTAAG AAATGGCACC ATTTCCAACA GGGTCCAAGC TATTGCCATA 
GGTCAGGCAC TCGTGGATGG CCGCTGGCTC GACTGTGTCA CCCACCACGA TCAGCTATTC 
AGAGATGAGT ATGCTCTCTA TCGACCACTT CAGAGTACAG AGTTCTCAGA AACTCCCTCC 
CCGGACAGCG ATAGTGTCAA CTCACTAGAG GGACATTCAG AGCCGTCATG GTTCAAGGAC 
ATCAAATTCG ATGACAGCGA CACCGAGCAG CCCGCAGACG ACACAGAGTA CACCATGCCT 
AGTTCCGCTG GGCCCAGCAA GAGGACATCT GTCAGCAGCG TCCACTCAGC AGTAGACAGT 
GACTCAGCTG CCTCCATCAA CCTCAACATG GAGCAAAACA ATGTGAATTT TCGAATCAAG 
AAAGATTCCA AGTGTCCCCA GGCATTCCCC GCCCTGGAGC ACAAATCTGA AATTATGGCG 
TCTGAAGATG GAGGAGAGAA CATCCTTATA AGTGATGCCT TTATCAAAGA GTCTCTCTTT 
AACCGTCGTG TGGAGGAGAA GGCTAAAGAA ATGCTGTTTA CTCCACTGGG TTGGCACCAC 
AGCTCTCTGG ACCAGCTCAG AGAGGAGAAT GGAGAAAAGA AGGCTATGGA GAGGCTGCTC 
TCGGCAAATC ACAGCCACAT GATGGCGCTG CTGCAGCAGT TGCTCTACAG CGAGTCTTTG 
TCTCTGTCCT GGCGGGATAT TATCGTTCCT GTGGTTCGCC AGGTTGTCCA GACAGTTCGA 
CCTGATGTTC GCAACAGTGA TGACGACATG GACATCCGCC AACTGGTCCA TATCAAAAAG 
ATTCCTGGAG GAAAAAAGTT TGACTCAACT GTAGTGAATG GCTTTGTGTG TACGAAGAGT 
ATTGCCCACA AAAAGATGAA CCCATATATC AAGAACCCCA AAATTCTGCT GCTGAAATGC 
TCAATAGAGT ATCTGTACAG GGAAGAGACC AAATTCACGT CCATCGACCC CATCGTGCTG 
CAGGAACGAG AGTTTCTGAA AAACTATGTG CAACGTATCG TGGATGTTCG GCCCACTCTG 
GTTCTGGTGG AAAAAACGGT GTCTCGTATA GCTCAGGACA TGCTGCTAGA ACACGGCATT 
ACCCTGGTCA TTAATGTCAA ACCGCAAGTA TTGGACAGGG TGAGTTGTGT GACCCAGGGG 
GACCTTGTCA TGTCCATGGA CCAGCTACTC ACCAAACCTC GTCTGGGTAC CTGCCACAAG 
TTTTACATGC AGCCCTTCCC TTTGGCCAAT AATGAGGTCA AGACTCTGAT GTTCTTCGAA 
GGATGTCCTG CTCATCTCGG GTGCTCCATA AATCTACGTG GTGCTTCAGA CTACGAGTTA 
GCCAGGGTGA AGGAGATCAT GCGATTAATG GTGTGTGTGG CCTACCACTC TCAGCTGGAG 
ATCTCCTTCC TCATGGATGA GTTTGCCATG CCGCCCAGCT TGTCCCAGAG CACTTCATTT 
CCCTGTCTCC TCGACGGAGG CTCTGCAGAT GAGGAAGCAA GCAGGGAGAA ACTAGAGAAG 
AGTTCTGAAG ATGAAGACAC TGTAGTGGAA GGACAACCTG AGGAGGTGCC CTCTGCCAAT 
GATGTAGACA CTTCCCAGGA CACAAATGAT CCTAAATTAT TCTCCTCTAT GGTGGAAGAG 
ACCACTAATA CTGACACAGT CACGACAAAG TCTTCCACTC CGTTCCAAAG CCCTCGTGCG 
CCATGCTTGC CAGTTCCTCC ACCATTCATC ATGGAAGAAA CCCCAGAAAT GGGCTCAGAC 
AGCCTAACAG GGGCATTTGA GGCAGGGGTG GTGGAATGCT GCCCAAAGGA GGAATCAACC 
AACCTAGAAG ATGGAGAAGA TTCTGATATT CCTACTCCTA AATTGTTCCG AGACCCCCTG 
CAAGATGATA CTGGGATGTT TGTGGCTGAA GAAGTTGCTT CTTCAGATGA CCATCTCAAG 
TCCATCTCTG CTATCTTCAA ACAGGAACTT AAGGACATCA TCCTGTGCAT TTCACCTTTC 
ATCACATTTG AGAAACCATT TCTTCTTACC CCTGCTGGGA TGCACTGCCC GAGCAGGGAT 
TATTTTCCCG AACAGGTCTA TCTGTCTCCT CTCCTCAACA AGGACTTTAA AGAGTTGGAT 
GGACGCAGAA AGCGGCAGCT CCTTAAGGAG TCCACTCCCT CCCTGGCAAA CAGCCTAACG 
AATGGTGGCC CCCCGTCAAA GCATGTCAGT GTCCTGCCAT CCCACAGTCT CACCAGCACC 
CGGATCGTTG AACTAGTAAA CAGGAGCCAG GGTCTGGCCA GAATGGTTGC AGACTACAGA 
GCAAAGGGTG GACGTATCCA GCACAGCGAA GCTACTGATC CATTTTGCAT AAGCAGCTCT 
TCTGCTCCGG TCAGTGGCCA GAATAGACCA GGTGATGGTT CGGTCAGAGC ACCAGTAAAG 
ACTGACAGCG AAGAGGAGAA GCCAACTAAA CCCAGTGACT GGACCCCCAA GCTGGATTGT 
CTGAACCCCA CCAACCACCA GAGACTCTGT GTGCTTTTTA GCAGTTCATC AACTCAGTCC 
AGCAATGCCC CCAACCCCTG TGTCAACCCA TGGATTGTTA CCATGGAGTT TTATGGCAAG 
AATGACCTCT GCCTTGGTGT ATTCTTGGAG CGATATTGCT TTAGGCCATC ATACCAGTGC 
CCCAGTATAT TTTGTGAGAC CCCCATGGTG CACCACATCC GGCGTTTTGT CCATGGCAAC 
GGCTGCGTGC AGATAGTACT GAAGGAGCTG GACTCCCCTG TGCCGGGGTA CCAGCACACC 
ATTCTCAACT ACTCCTGGTG CCGCATCTGC AAACAAGTGA CACCAGTGGT TCCTCTATCC 
AATGACTCAT GGTCAATGTC TTTTGCAAAA TATCTGGAGC TTCGCTTCTA CGGCCACCAG 
TACACCAGGA GGGCCAATGC AGAGCCCTGT GGCCACTCCA TCCACAAAGA CTACCACCAG 
TACTTCTCAT ACAACCAAAT GGTGGCTTCC TTCAGTTACA CCTCAGTGAG GCTGCTGGAG 
ATTTGTCTTC CTCGTTTCAA AATCTTCATC AGGAATCAGG GTCCTTCGAT CAACAACTTG 
GGGCAGGATC TGAAAGACTT CTCTCAAAAA GTAAATCAGG TTTACGTGGC CATAGATGAT 
CGTCTCACAT CTCTAAAGAC GGACACCTTC AGTAAAACAC GCGAGGAGAA GATGGAGGAC 
CTCTTTGCAC AGAAAGATAT GGAAGAGGCG GAACTGCGCA GCTGGATTGA AAAGTTTCAA 
GCACGATTGC AGGCTTGTGG TCACGTTTCT CCTCAGCAGC TGCAGACGAC TCTGGAATCA 
CTGGTGATGA AGAAACAGAG TCTGTGTGAG ATGTTGCAGT ACTGGAACAG CAGGCTGCAG 
GAATTATTCC AACAGGAGAA AGGCAGGAAA CGTCTGTCAG TGCCCCCAAG CCCCGGCAGG 
CACAGACAGA CTGCCCCTGA TGATGGCAAG AACGCTGTGG AGTCATCCCC ACGTAACCCT 
TCACCTGTGG TACAGAATGG TGAAAAGGAG GACCGTCACC TCACCACACT GCCTTCAACC 
TCCTCTCTGT TGCCCTCGCC TGGAGACCTG GGAGCTGAAC CAGCCACATC TGGTCCTTCC 
CTGAGTGAGC AGGACTCTGT CAGCATCCCA GAAGATGTAT TTGATGGACA CTTGCTGGGC 
TCCAATGACA GTCAGGTGAA GGAGAAATCC ACCATGAAAG CCATCCTTGC CAACTTCCTG 
CCCGGCAACA GTTACAACCC CATTCCATTC CCATTTGACC CAGACAAACA CTACCTGATG 
GATGAGTATG AGCGAGTGCC AATTGCAGTG TGTGAAAGAG AACCAAGCTC CATCATTGCA 
TTTGCTCTGA GTTGTAAGAA GTATAAAACA GCACTGGAAG ATCTGTCCAA GGTGTCAAAC 
ATGGGTGGAG AAGACTCTTC ACAAGCTGCC AGTAGTGGAG AGAGTCGGGT GAGGGGCAGC 
CCCGCCAGGC CCAGTGAGTC AGCCTCGTCC CAACAGAGCC GCAGCAGCAG TGAAGCAGAT 
CCCCTCAAAG ACGCAGACCT AACAGACAAA CAGAAGAAGC AGGCCCTGAA TCCCCACATT 
GAGCTACAGT TCTCTGATGC AAGCGCAAAG TTTTACTGTC GGATCTACTA CGCCGAGGAG 
TTTCATAAGA TGCGTGAGGA GATCATGGAG AGCTCCGAAG AGGACTTTGT GCGTTCCCTG 
TCGCACTGTG TCAACTGGCA GGCGCGCGGT GGAAAGTCTG GGGCCGTTTT CTACGCAACA 
GAAGACGACC GTTTCATCCT AAAACAGATG CCCAGGCTGG AGGTGCAGTC CTTCTTGGAC 
TTTGCTCCGC ACTACTTCAC CTACATCACC GGAGCTGTGC AGCAGAAGAG GCCAACTGCA 
TTGGCAAAGA TTCTCGGAGT TTACAGGATA GGCTACAAGA ACTCCCAGAA CAACACTGAG 
AAGAAACTGG ACCTGCTCGT CATGGAAAAC CTGTTCTACG GACGCAAGAT GGCTCAGGTC 
TTTGACCTGA AAGGTTCCCT CAGAAACCGT CATGTGAAGA CAGACCTGGT AAAGGAAAGC 
TGCGAGGTGG TCCTGCTGGA TGAGAATCTA CTGAAGCTGA TCCACGACAA TCCGCTCTAC 
ATTCGCTCTC ACTGCAAGTC CATCTTGCGC GCCGCCATAC ACAGCGACGC CTACTTCCTG 
TCCAGCCACC TCATCATTGA CTACTCCCTG CTGGTGGGGT GGGACGATGC CACCGACCAG 
CTGGTGGTTG GGATCATAGA CTATATTCGG ACGTTTACCT GGGACAAGAA ATTAGAGATG 
GTTGTCAAAT CCACTGGAAT CCTGGGGGGT CAGGGAAAGA TGCCCACCGT TGTTTCTCCT 
GAGCTGTACC GAGCTCGGTT CTGTGAGGCC ATGGACAAAT ACTTCCTGAT GGTACCCGAC 
CACTGGACGG GTCTTGGCAT CAACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_003961636.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003961685.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.

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CloneID	OTo00681
Clone ID Related Accession (Same CDS sequence)	XM_003961636.2
Accession Version	XM_003961636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003961636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GAGGAGGGAC GTTCTCTTTC AACAGTGTCA GAGAAATCAG ACACTCCATC CCCTTCCCCT 
CTATCAGAGA GGAGGAGCTG GTCCTCCAGC CCACCCCATT CCCTCTACAC TGCCAGGATT 
CCCCGAAAAC AACCAGATCA CCTTCAAAGG ACCTCTGCTG CTTCAGATGG CAGCAGGAAA 
TCAGAGACTC CTTTAAGCAA TCACGACCCT CGAACTGCTG TGCAACTTCG CACTGCATTG 
AAGAGGCTGA AGGAAATAAT GGAGGGAAAG AGCCAGGACA GTGACTTGAA GCAGTACTGG 
ATGCCGGACA GCCAGTGTAA AGAATGTTAT GACTGCAATG AAAAGTTCAC AACCTTCCGA 
CGCCGCCACC ATTGCCGACT GTGTGGTCAG ATTTTCTGCA GTCGCTGCTG CAACCAGGAA 
ATCCCTGGAA AGTTCATGGG TTACACAGGA GATTTGCGGG CCTGTACGTA CTGCCGTAAG 
TTGGCACTGA GCTACGCCCA CTCGGCTGAT TCGGGCTCCA TCGGCGAAGA CTTGAACACC 
CTGACTGACT CTCCGAGCTC TGTGTGTGTG CTGGAGCCCA GTGAGCCACG GACGCCTGTG 
GGGGGGCGCA AGGCCAGCAG GAACATCTTC CTAGAGGAAG ACTTAACATG GCAGAGAAAA 
ACACCTATTG GGATGAGAAA GAACATGATT CACCAGGAAC CTCAGAGCAG CGGCCTCAGT 
TCCAGACTGA CAGGACTTCA GGAGGATGTA GGAAAATCAC CTGGTAGGAG GAGGTCTGCT 
AGCATCAGCA CACTTCCACT TGACCGCTCT GGGTCATCAA TGGTACCTTC CTACGACAGC 
TCCGTCAGCC CCCCTACCAG CAGAGCCATG TCGGTGTCAA AGGCTGGGAC AAAATTAGAT 
CACAGTGAGG AGGAACGTAA AATACTACTG GACTCCTCCC AGTTGAAGGA CTTATGGAGG 
AAAATCTGTC ATAATGGAAC AGGGATGGAA TTCCAGGACC ACAGATACTG GCTGAGAACC 
TACCCCAACT GCATTGTGGG AAAAGAGCTC GTCAACTGGC TGCTAAGAAA TGGCACCATT 
TCCAACAGGG TCCAAGCTAT TGCCATAGGT CAGGCACTCG TGGATGGCCG CTGGCTCGAC 
TGTGTCACCC ACCACGATCA GCTATTCAGA GATGAGTATG CTCTCTATCG ACCACTTCAG 
AGTACAGAGT TCTCAGAAAC TCCCTCCCCG GACAGCGATA GTGTCAACTC ACTAGAGGGA 
CATTCAGAGC CGTCATGGTT CAAGGACATC AAATTCGATG ACAGCGACAC CGAGCAGCCC 
GCAGACGACA CAGAGTACAC CATGCCTAGT TCCGCTGGGC CCAGCAAGAG GACATCTGTC 
AGCAGCGTCC ACTCAGCAGT AGACAGTGAC TCAGCTGCCT CCATCAACCT CAACATGGAG 
CAAAACAATG TGAATTTTCG AATCAAGAAA GATTCCAAGT GTCCCCAGGC ATTCCCCGCC 
CTGGAGCACA AATCTGAAAT TATGGCGTCT GAAGATGGAG GAGAGAACAT CCTTATAAGT 
GATGCCTTTA TCAAAGAGTC TCTCTTTAAC CGTCGTGTGG AGGAGAAGGC TAAAGAAATG 
CTGTTTACTC CACTGGGTTG GCACCACAGC TCTCTGGACC AGCTCAGAGA GGAGAATGGA 
GAAAAGAAGG CTATGGAGAG GCTGCTCTCG GCAAATCACA GCCACATGAT GGCGCTGCTG 
CAGCAGTTGC TCTACAGCGA GTCTTTGTCT CTGTCCTGGC GGGATATTAT CGTTCCTGTG 
GTTCGCCAGG TTGTCCAGAC AGTTCGACCT GATGTTCGCA ACAGTGATGA CGACATGGAC 
ATCCGCCAAC TGGTCCATAT CAAAAAGATT CCTGGAGGAA AAAAGTTTGA CTCAACTGTA 
GTGAATGGCT TTGTGTGTAC GAAGAGTATT GCCCACAAAA AGATGAACCC ATATATCAAG 
AACCCCAAAA TTCTGCTGCT GAAATGCTCA ATAGAGTATC TGTACAGGGA AGAGACCAAA 
TTCACGTCCA TCGACCCCAT CGTGCTGCAG GAACGAGAGT TTCTGAAAAA CTATGTGCAA 
CGTATCGTGG ATGTTCGGCC CACTCTGGTT CTGGTGGAAA AAACGGTGTC TCGTATAGCT 
CAGGACATGC TGCTAGAACA CGGCATTACC CTGGTCATTA ATGTCAAACC GCAAGTATTG 
GACAGGGTGA GTTGTGTGAC CCAGGGGGAC CTTGTCATGT CCATGGACCA GCTACTCACC 
AAACCTCGTC TGGGTACCTG CCACAAGTTT TACATGCAGC CCTTCCCTTT GGCCAATAAT 
GAGGTCAAGA CTCTGATGTT CTTCGAAGGA TGTCCTGCTC ATCTCGGGTG CTCCATAAAT 
CTACGTGGTG CTTCAGACTA CGAGTTAGCC AGGGTGAAGG AGATCATGCG ATTAATGGTG 
TGTGTGGCCT ACCACTCTCA GCTGGAGATC TCCTTCCTCA TGGATGAGTT TGCCATGCCG 
CCCAGCTTGT CCCAGAGCAC TTCATTTCCC TGTCTCCTCG ACGGAGGCTC TGCAGATGAG 
GAAGCAAGCA GGGAGAAACT AGAGAAGAGT TCTGAAGATG AAGACACTGT AGTGGAAGGA 
CAACCTGAGG AGGTGCCCTC TGCCAATGAT GTAGACACTT CCCAGGACAC AAATGATCCT 
AAATTATTCT CCTCTATGGT GGAAGAGACC ACTAATACTG ACACAGTCAC GACAAAGTCT 
TCCACTCCGT TCCAAAGCCC TCGTGCGCCA TGCTTGCCAG TTCCTCCACC ATTCATCATG 
GAAGAAACCC CAGAAATGGG CTCAGACAGC CTAACAGGGG CATTTGAGGC AGGGGTGGTG 
GAATGCTGCC CAAAGGAGGA ATCAACCAAC CTAGAAGATG GAGAAGATTC TGATATTCCT 
ACTCCTAAAT TGTTCCGAGA CCCCCTGCAA GATGATACTG GGATGTTTGT GGCTGAAGAA 
GTTGCTTCTT CAGATGACCA TCTCAAGTCC ATCTCTGCTA TCTTCAAACA GGAACTTAAG 
GACATCATCC TGTGCATTTC ACCTTTCATC ACATTTGAGA AACCATTTCT TCTTACCCCT 
GCTGGGATGC ACTGCCCGAG CAGGGATTAT TTTCCCGAAC AGGTCTATCT GTCTCCTCTC 
CTCAACAAGG ACTTTAAAGA GTTGGATGGA CGCAGAAAGC GGCAGCTCCT TAAGGAGTCC 
ACTCCCTCCC TGGCAAACAG CCTAACGAAT GGTGGCCCCC CGTCAAAGCA TGTCAGTGTC 
CTGCCATCCC ACAGTCTCAC CAGCACCCGG ATCGTTGAAC TAGTAAACAG GAGCCAGGGT 
CTGGCCAGAA TGGTTGCAGA CTACAGAGCA AAGGGTGGAC GTATCCAGCA CAGCGAAGCT 
ACTGATCCAT TTTGCATAAG CAGCTCTTCT GCTCCGGTCA GTGGCCAGAA TAGACCAGGT 
GATGGTTCGG TCAGAGCACC AGTAAAGACT GACAGCGAAG AGGAGAAGCC AACTAAACCC 
AGTGACTGGA CCCCCAAGCT GGATTGTCTG AACCCCACCA ACCACCAGAG ACTCTGTGTG 
CTTTTTAGCA GTTCATCAAC TCAGTCCAGC AATGCCCCCA ACCCCTGTGT CAACCCATGG 
ATTGTTACCA TGGAGTTTTA TGGCAAGAAT GACCTCTGCC TTGGTGTATT CTTGGAGCGA 
TATTGCTTTA GGCCATCATA CCAGTGCCCC AGTATATTTT GTGAGACCCC CATGGTGCAC 
CACATCCGGC GTTTTGTCCA TGGCAACGGC TGCGTGCAGA TAGTACTGAA GGAGCTGGAC 
TCCCCTGTGC CGGGGTACCA GCACACCATT CTCAACTACT CCTGGTGCCG CATCTGCAAA 
CAAGTGACAC CAGTGGTTCC TCTATCCAAT GACTCATGGT CAATGTCTTT TGCAAAATAT 
CTGGAGCTTC GCTTCTACGG CCACCAGTAC ACCAGGAGGG CCAATGCAGA GCCCTGTGGC 
CACTCCATCC ACAAAGACTA CCACCAGTAC TTCTCATACA ACCAAATGGT GGCTTCCTTC 
AGTTACACCT CAGTGAGGCT GCTGGAGATT TGTCTTCCTC GTTTCAAAAT CTTCATCAGG 
AATCAGGGTC CTTCGATCAA CAACTTGGGG CAGGATCTGA AAGACTTCTC TCAAAAAGTA 
AATCAGGTTT ACGTGGCCAT AGATGATCGT CTCACATCTC TAAAGACGGA CACCTTCAGT 
AAAACACGCG AGGAGAAGAT GGAGGACCTC TTTGCACAGA AAGATATGGA AGAGGCGGAA 
CTGCGCAGCT GGATTGAAAA GTTTCAAGCA CGATTGCAGG CTTGTGGTCA CGTTTCTCCT 
CAGCAGCTGC AGACGACTCT GGAATCACTG GTGATGAAGA AACAGAGTCT GTGTGAGATG 
TTGCAGTACT GGAACAGCAG GCTGCAGGAA TTATTCCAAC AGGAGAAAGG CAGGAAACGT 
CTGTCAGTGC CCCCAAGCCC CGGCAGGCAC AGACAGACTG CCCCTGATGA TGGCAAGAAC 
GCTGTGGAGT CATCCCCACG TAACCCTTCA CCTGTGGTAC AGAATGGTGA AAAGGAGGAC 
CGTCACCTCA CCACACTGCC TTCAACCTCC TCTCTGTTGC CCTCGCCTGG AGACCTGGGA 
GCTGAACCAG CCACATCTGG TCCTTCCCTG AGTGAGCAGG ACTCTGTCAG CATCCCAGAA 
GATGTATTTG ATGGACACTT GCTGGGCTCC AATGACAGTC AGGTGAAGGA GAAATCCACC 
ATGAAAGCCA TCCTTGCCAA CTTCCTGCCC GGCAACAGTT ACAACCCCAT TCCATTCCCA 
TTTGACCCAG ACAAACACTA CCTGATGGAT GAGTATGAGC GAGTGCCAAT TGCAGTGTGT 
GAAAGAGAAC CAAGCTCCAT CATTGCATTT GCTCTGAGTT GTAAGAAGTA TAAAACAGCA 
CTGGAAGATC TGTCCAAGGT GTCAAACATG GGTGGAGAAG ACTCTTCACA AGCTGCCAGT 
AGTGGAGAGA GTCGGGTGAG GGGCAGCCCC GCCAGGCCCA GTGAGTCAGC CTCGTCCCAA 
CAGAGCCGCA GCAGCAGTGA AGCAGATCCC CTCAAAGACG CAGACCTAAC AGACAAACAG 
AAGAAGCAGG CCCTGAATCC CCACATTGAG CTACAGTTCT CTGATGCAAG CGCAAAGTTT 
TACTGTCGGA TCTACTACGC CGAGGAGTTT CATAAGATGC GTGAGGAGAT CATGGAGAGC 
TCCGAAGAGG ACTTTGTGCG TTCCCTGTCG CACTGTGTCA ACTGGCAGGC GCGCGGTGGA 
AAGTCTGGGG CCGTTTTCTA CGCAACAGAA GACGACCGTT TCATCCTAAA ACAGATGCCC 
AGGCTGGAGG TGCAGTCCTT CTTGGACTTT GCTCCGCACT ACTTCACCTA CATCACCGGA 
GCTGTGCAGC AGAAGAGGCC AACTGCATTG GCAAAGATTC TCGGAGTTTA CAGGATAGGC 
TACAAGAACT CCCAGAACAA CACTGAGAAG AAACTGGACC TGCTCGTCAT GGAAAACCTG 
TTCTACGGAC GCAAGATGGC TCAGGTCTTT GACCTGAAAG GTTCCCTCAG AAACCGTCAT 
GTGAAGACAG ACCTGGTAAA GGAAAGCTGC GAGGTGGTCC TGCTGGATGA GAATCTACTG 
AAGCTGATCC ACGACAATCC GCTCTACATT CGCTCTCACT GCAAGTCCAT CTTGCGCGCC 
GCCATACACA GCGACGCCTA CTTCCTGTCC AGCCACCTCA TCATTGACTA CTCCCTGCTG 
GTGGGGTGGG ACGATGCCAC CGACCAGCTG GTGGTTGGGA TCATAGACTA TATTCGGACG 
TTTACCTGGG ACAAGAAATT AGAGATGGTT GTCAAATCCA CTGGAATCCT GGGGGGTCAG 
GGAAAGATGC CCACCGTTGT TTCTCCTGAG CTGTACCGAG CTCGGTTCTG TGAGGCCATG 
GACAAATACT TCCTGATGGT ACCCGACCAC TGGACGGGTC TTGGCATCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961685.1
CDS	123..6359
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKEEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_003961636.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961636.2

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GAGGAGGGAC GTTCTCTTTC AACAGTGTCA GAGAAATCAG ACACTCCATC CCCTTCCCCT 
CTATCAGAGA GGAGGAGCTG GTCCTCCAGC CCACCCCATT CCCTCTACAC TGCCAGGATT 
CCCCGAAAAC AACCAGATCA CCTTCAAAGG ACCTCTGCTG CTTCAGATGG CAGCAGGAAA 
TCAGAGACTC CTTTAAGCAA TCACGACCCT CGAACTGCTG TGCAACTTCG CACTGCATTG 
AAGAGGCTGA AGGAAATAAT GGAGGGAAAG AGCCAGGACA GTGACTTGAA GCAGTACTGG 
ATGCCGGACA GCCAGTGTAA AGAATGTTAT GACTGCAATG AAAAGTTCAC AACCTTCCGA 
CGCCGCCACC ATTGCCGACT GTGTGGTCAG ATTTTCTGCA GTCGCTGCTG CAACCAGGAA 
ATCCCTGGAA AGTTCATGGG TTACACAGGA GATTTGCGGG CCTGTACGTA CTGCCGTAAG 
TTGGCACTGA GCTACGCCCA CTCGGCTGAT TCGGGCTCCA TCGGCGAAGA CTTGAACACC 
CTGACTGACT CTCCGAGCTC TGTGTGTGTG CTGGAGCCCA GTGAGCCACG GACGCCTGTG 
GGGGGGCGCA AGGCCAGCAG GAACATCTTC CTAGAGGAAG ACTTAACATG GCAGAGAAAA 
ACACCTATTG GGATGAGAAA GAACATGATT CACCAGGAAC CTCAGAGCAG CGGCCTCAGT 
TCCAGACTGA CAGGACTTCA GGAGGATGTA GGAAAATCAC CTGGTAGGAG GAGGTCTGCT 
AGCATCAGCA CACTTCCACT TGACCGCTCT GGGTCATCAA TGGTACCTTC CTACGACAGC 
TCCGTCAGCC CCCCTACCAG CAGAGCCATG TCGGTGTCAA AGGCTGGGAC AAAATTAGAT 
CACAGTGAGG AGGAACGTAA AATACTACTG GACTCCTCCC AGTTGAAGGA CTTATGGAGG 
AAAATCTGTC ATAATGGAAC AGGGATGGAA TTCCAGGACC ACAGATACTG GCTGAGAACC 
TACCCCAACT GCATTGTGGG AAAAGAGCTC GTCAACTGGC TGCTAAGAAA TGGCACCATT 
TCCAACAGGG TCCAAGCTAT TGCCATAGGT CAGGCACTCG TGGATGGCCG CTGGCTCGAC 
TGTGTCACCC ACCACGATCA GCTATTCAGA GATGAGTATG CTCTCTATCG ACCACTTCAG 
AGTACAGAGT TCTCAGAAAC TCCCTCCCCG GACAGCGATA GTGTCAACTC ACTAGAGGGA 
CATTCAGAGC CGTCATGGTT CAAGGACATC AAATTCGATG ACAGCGACAC CGAGCAGCCC 
GCAGACGACA CAGAGTACAC CATGCCTAGT TCCGCTGGGC CCAGCAAGAG GACATCTGTC 
AGCAGCGTCC ACTCAGCAGT AGACAGTGAC TCAGCTGCCT CCATCAACCT CAACATGGAG 
CAAAACAATG TGAATTTTCG AATCAAGAAA GATTCCAAGT GTCCCCAGGC ATTCCCCGCC 
CTGGAGCACA AATCTGAAAT TATGGCGTCT GAAGATGGAG GAGAGAACAT CCTTATAAGT 
GATGCCTTTA TCAAAGAGTC TCTCTTTAAC CGTCGTGTGG AGGAGAAGGC TAAAGAAATG 
CTGTTTACTC CACTGGGTTG GCACCACAGC TCTCTGGACC AGCTCAGAGA GGAGAATGGA 
GAAAAGAAGG CTATGGAGAG GCTGCTCTCG GCAAATCACA GCCACATGAT GGCGCTGCTG 
CAGCAGTTGC TCTACAGCGA GTCTTTGTCT CTGTCCTGGC GGGATATTAT CGTTCCTGTG 
GTTCGCCAGG TTGTCCAGAC AGTTCGACCT GATGTTCGCA ACAGTGATGA CGACATGGAC 
ATCCGCCAAC TGGTCCATAT CAAAAAGATT CCTGGAGGAA AAAAGTTTGA CTCAACTGTA 
GTGAATGGCT TTGTGTGTAC GAAGAGTATT GCCCACAAAA AGATGAACCC ATATATCAAG 
AACCCCAAAA TTCTGCTGCT GAAATGCTCA ATAGAGTATC TGTACAGGGA AGAGACCAAA 
TTCACGTCCA TCGACCCCAT CGTGCTGCAG GAACGAGAGT TTCTGAAAAA CTATGTGCAA 
CGTATCGTGG ATGTTCGGCC CACTCTGGTT CTGGTGGAAA AAACGGTGTC TCGTATAGCT 
CAGGACATGC TGCTAGAACA CGGCATTACC CTGGTCATTA ATGTCAAACC GCAAGTATTG 
GACAGGGTGA GTTGTGTGAC CCAGGGGGAC CTTGTCATGT CCATGGACCA GCTACTCACC 
AAACCTCGTC TGGGTACCTG CCACAAGTTT TACATGCAGC CCTTCCCTTT GGCCAATAAT 
GAGGTCAAGA CTCTGATGTT CTTCGAAGGA TGTCCTGCTC ATCTCGGGTG CTCCATAAAT 
CTACGTGGTG CTTCAGACTA CGAGTTAGCC AGGGTGAAGG AGATCATGCG ATTAATGGTG 
TGTGTGGCCT ACCACTCTCA GCTGGAGATC TCCTTCCTCA TGGATGAGTT TGCCATGCCG 
CCCAGCTTGT CCCAGAGCAC TTCATTTCCC TGTCTCCTCG ACGGAGGCTC TGCAGATGAG 
GAAGCAAGCA GGGAGAAACT AGAGAAGAGT TCTGAAGATG AAGACACTGT AGTGGAAGGA 
CAACCTGAGG AGGTGCCCTC TGCCAATGAT GTAGACACTT CCCAGGACAC AAATGATCCT 
AAATTATTCT CCTCTATGGT GGAAGAGACC ACTAATACTG ACACAGTCAC GACAAAGTCT 
TCCACTCCGT TCCAAAGCCC TCGTGCGCCA TGCTTGCCAG TTCCTCCACC ATTCATCATG 
GAAGAAACCC CAGAAATGGG CTCAGACAGC CTAACAGGGG CATTTGAGGC AGGGGTGGTG 
GAATGCTGCC CAAAGGAGGA ATCAACCAAC CTAGAAGATG GAGAAGATTC TGATATTCCT 
ACTCCTAAAT TGTTCCGAGA CCCCCTGCAA GATGATACTG GGATGTTTGT GGCTGAAGAA 
GTTGCTTCTT CAGATGACCA TCTCAAGTCC ATCTCTGCTA TCTTCAAACA GGAACTTAAG 
GACATCATCC TGTGCATTTC ACCTTTCATC ACATTTGAGA AACCATTTCT TCTTACCCCT 
GCTGGGATGC ACTGCCCGAG CAGGGATTAT TTTCCCGAAC AGGTCTATCT GTCTCCTCTC 
CTCAACAAGG ACTTTAAAGA GTTGGATGGA CGCAGAAAGC GGCAGCTCCT TAAGGAGTCC 
ACTCCCTCCC TGGCAAACAG CCTAACGAAT GGTGGCCCCC CGTCAAAGCA TGTCAGTGTC 
CTGCCATCCC ACAGTCTCAC CAGCACCCGG ATCGTTGAAC TAGTAAACAG GAGCCAGGGT 
CTGGCCAGAA TGGTTGCAGA CTACAGAGCA AAGGGTGGAC GTATCCAGCA CAGCGAAGCT 
ACTGATCCAT TTTGCATAAG CAGCTCTTCT GCTCCGGTCA GTGGCCAGAA TAGACCAGGT 
GATGGTTCGG TCAGAGCACC AGTAAAGACT GACAGCGAAG AGGAGAAGCC AACTAAACCC 
AGTGACTGGA CCCCCAAGCT GGATTGTCTG AACCCCACCA ACCACCAGAG ACTCTGTGTG 
CTTTTTAGCA GTTCATCAAC TCAGTCCAGC AATGCCCCCA ACCCCTGTGT CAACCCATGG 
ATTGTTACCA TGGAGTTTTA TGGCAAGAAT GACCTCTGCC TTGGTGTATT CTTGGAGCGA 
TATTGCTTTA GGCCATCATA CCAGTGCCCC AGTATATTTT GTGAGACCCC CATGGTGCAC 
CACATCCGGC GTTTTGTCCA TGGCAACGGC TGCGTGCAGA TAGTACTGAA GGAGCTGGAC 
TCCCCTGTGC CGGGGTACCA GCACACCATT CTCAACTACT CCTGGTGCCG CATCTGCAAA 
CAAGTGACAC CAGTGGTTCC TCTATCCAAT GACTCATGGT CAATGTCTTT TGCAAAATAT 
CTGGAGCTTC GCTTCTACGG CCACCAGTAC ACCAGGAGGG CCAATGCAGA GCCCTGTGGC 
CACTCCATCC ACAAAGACTA CCACCAGTAC TTCTCATACA ACCAAATGGT GGCTTCCTTC 
AGTTACACCT CAGTGAGGCT GCTGGAGATT TGTCTTCCTC GTTTCAAAAT CTTCATCAGG 
AATCAGGGTC CTTCGATCAA CAACTTGGGG CAGGATCTGA AAGACTTCTC TCAAAAAGTA 
AATCAGGTTT ACGTGGCCAT AGATGATCGT CTCACATCTC TAAAGACGGA CACCTTCAGT 
AAAACACGCG AGGAGAAGAT GGAGGACCTC TTTGCACAGA AAGATATGGA AGAGGCGGAA 
CTGCGCAGCT GGATTGAAAA GTTTCAAGCA CGATTGCAGG CTTGTGGTCA CGTTTCTCCT 
CAGCAGCTGC AGACGACTCT GGAATCACTG GTGATGAAGA AACAGAGTCT GTGTGAGATG 
TTGCAGTACT GGAACAGCAG GCTGCAGGAA TTATTCCAAC AGGAGAAAGG CAGGAAACGT 
CTGTCAGTGC CCCCAAGCCC CGGCAGGCAC AGACAGACTG CCCCTGATGA TGGCAAGAAC 
GCTGTGGAGT CATCCCCACG TAACCCTTCA CCTGTGGTAC AGAATGGTGA AAAGGAGGAC 
CGTCACCTCA CCACACTGCC TTCAACCTCC TCTCTGTTGC CCTCGCCTGG AGACCTGGGA 
GCTGAACCAG CCACATCTGG TCCTTCCCTG AGTGAGCAGG ACTCTGTCAG CATCCCAGAA 
GATGTATTTG ATGGACACTT GCTGGGCTCC AATGACAGTC AGGTGAAGGA GAAATCCACC 
ATGAAAGCCA TCCTTGCCAA CTTCCTGCCC GGCAACAGTT ACAACCCCAT TCCATTCCCA 
TTTGACCCAG ACAAACACTA CCTGATGGAT GAGTATGAGC GAGTGCCAAT TGCAGTGTGT 
GAAAGAGAAC CAAGCTCCAT CATTGCATTT GCTCTGAGTT GTAAGAAGTA TAAAACAGCA 
CTGGAAGATC TGTCCAAGGT GTCAAACATG GGTGGAGAAG ACTCTTCACA AGCTGCCAGT 
AGTGGAGAGA GTCGGGTGAG GGGCAGCCCC GCCAGGCCCA GTGAGTCAGC CTCGTCCCAA 
CAGAGCCGCA GCAGCAGTGA AGCAGATCCC CTCAAAGACG CAGACCTAAC AGACAAACAG 
AAGAAGCAGG CCCTGAATCC CCACATTGAG CTACAGTTCT CTGATGCAAG CGCAAAGTTT 
TACTGTCGGA TCTACTACGC CGAGGAGTTT CATAAGATGC GTGAGGAGAT CATGGAGAGC 
TCCGAAGAGG ACTTTGTGCG TTCCCTGTCG CACTGTGTCA ACTGGCAGGC GCGCGGTGGA 
AAGTCTGGGG CCGTTTTCTA CGCAACAGAA GACGACCGTT TCATCCTAAA ACAGATGCCC 
AGGCTGGAGG TGCAGTCCTT CTTGGACTTT GCTCCGCACT ACTTCACCTA CATCACCGGA 
GCTGTGCAGC AGAAGAGGCC AACTGCATTG GCAAAGATTC TCGGAGTTTA CAGGATAGGC 
TACAAGAACT CCCAGAACAA CACTGAGAAG AAACTGGACC TGCTCGTCAT GGAAAACCTG 
TTCTACGGAC GCAAGATGGC TCAGGTCTTT GACCTGAAAG GTTCCCTCAG AAACCGTCAT 
GTGAAGACAG ACCTGGTAAA GGAAAGCTGC GAGGTGGTCC TGCTGGATGA GAATCTACTG 
AAGCTGATCC ACGACAATCC GCTCTACATT CGCTCTCACT GCAAGTCCAT CTTGCGCGCC 
GCCATACACA GCGACGCCTA CTTCCTGTCC AGCCACCTCA TCATTGACTA CTCCCTGCTG 
GTGGGGTGGG ACGATGCCAC CGACCAGCTG GTGGTTGGGA TCATAGACTA TATTCGGACG 
TTTACCTGGG ACAAGAAATT AGAGATGGTT GTCAAATCCA CTGGAATCCT GGGGGGTCAG 
GGAAAGATGC CCACCGTTGT TTCTCCTGAG CTGTACCGAG CTCGGTTCTG TGAGGCCATG 
GACAAATACT TCCTGATGGT ACCCGACCAC TGGACGGGTC TTGGCATCAA CTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607487.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605789.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.

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CloneID	OTo31326
Clone ID Related Accession (Same CDS sequence)	XM_011607487.1 , XM_011607495.1
Accession Version	XM_011607487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA ATTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605789.1
CDS	123..6443
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607487.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607487.1

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA ATTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607495.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605797.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.

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CloneID	OTo31326
Clone ID Related Accession (Same CDS sequence)	XM_011607487.1 , XM_011607495.1
Accession Version	XM_011607495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA ATTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605797.1
CDS	120..6440
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607495.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607495.1

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA ATTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAGTAG GCGTCCAGAA 
GACGCAGACC TAACAGACAA ACAGAAGAAG CAGGCCCTGA ATCCCCACAT TGAGCTACAG 
TTCTCTGATG CAAGCGCAAA GTTTTACTGT CGGATCTACT ACGCCGAGGA GTTTCATAAG 
ATGCGTGAGG AGATCATGGA GAGCTCCGAA GAGGACTTTG TGCGTTCCCT GTCGCACTGT 
GTCAACTGGC AGGCGCGCGG TGGAAAGTCT GGGGCCGTTT TCTACGCAAC AGAAGACGAC 
CGTTTCATCC TAAAACAGAT GCCCAGGCTG GAGGTGCAGT CCTTCTTGGA CTTTGCTCCG 
CACTACTTCA CCTACATCAC CGGAGCTGTG CAGCAGAAGA GGCCAACTGC ATTGGCAAAG 
ATTCTCGGAG TTTACAGGAT AGGCTACAAG AACTCCCAGA ACAACACTGA GAAGAAACTG 
GACCTGCTCG TCATGGAAAA CCTGTTCTAC GGACGCAAGA TGGCTCAGGT CTTTGACCTG 
AAAGGTTCCC TCAGAAACCG TCATGTGAAG ACAGACCTGG TAAAGGAAAG CTGCGAGGTG 
GTCCTGCTGG ATGAGAATCT ACTGAAGCTG ATCCACGACA ATCCGCTCTA CATTCGCTCT 
CACTGCAAGT CCATCTTGCG CGCCGCCATA CACAGCGACG CCTACTTCCT GTCCAGCCAC 
CTCATCATTG ACTACTCCCT GCTGGTGGGG TGGGACGATG CCACCGACCA GCTGGTGGTT 
GGGATCATAG ACTATATTCG GACGTTTACC TGGGACAAGA AATTAGAGAT GGTTGTCAAA 
TCCACTGGAA TCCTGGGGGG TCAGGGAAAG ATGCCCACCG TTGTTTCTCC TGAGCTGTAC 
CGAGCTCGGT TCTGTGAGGC CATGGACAAA TACTTCCTGA TGGTACCCGA CCACTGGACG 
GGTCTTGGCA TCAACTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607503.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605805.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.

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CloneID	OTo31327
Clone ID Related Accession (Same CDS sequence)	XM_011607503.1
Accession Version	XM_011607503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA ATTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAAAGA CGCAGACCTA 
ACAGACAAAC AGAAGAAGCA GGCCCTGAAT CCCCACATTG AGCTACAGTT CTCTGATGCA 
AGCGCAAAGT TTTACTGTCG GATCTACTAC GCCGAGGAGT TTCATAAGAT GCGTGAGGAG 
ATCATGGAGA GCTCCGAAGA GGACTTTGTG CGTTCCCTGT CGCACTGTGT CAACTGGCAG 
GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC TACGCAACAG AAGACGACCG TTTCATCCTA 
AAACAGATGC CCAGGCTGGA GGTGCAGTCC TTCTTGGACT TTGCTCCGCA CTACTTCACC 
TACATCACCG GAGCTGTGCA GCAGAAGAGG CCAACTGCAT TGGCAAAGAT TCTCGGAGTT 
TACAGGATAG GCTACAAGAA CTCCCAGAAC AACACTGAGA AGAAACTGGA CCTGCTCGTC 
ATGGAAAACC TGTTCTACGG ACGCAAGATG GCTCAGGTCT TTGACCTGAA AGGTTCCCTC 
AGAAACCGTC ATGTGAAGAC AGACCTGGTA AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT 
GAGAATCTAC TGAAGCTGAT CCACGACAAT CCGCTCTACA TTCGCTCTCA CTGCAAGTCC 
ATCTTGCGCG CCGCCATACA CAGCGACGCC TACTTCCTGT CCAGCCACCT CATCATTGAC 
TACTCCCTGC TGGTGGGGTG GGACGATGCC ACCGACCAGC TGGTGGTTGG GATCATAGAC 
TATATTCGGA CGTTTACCTG GGACAAGAAA TTAGAGATGG TTGTCAAATC CACTGGAATC 
CTGGGGGGTC AGGGAAAGAT GCCCACCGTT GTTTCTCCTG AGCTGTACCG AGCTCGGTTC 
TGTGAGGCCA TGGACAAATA CTTCCTGATG GTACCCGACC ACTGGACGGG TCTTGGCATC 
AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605805.1
CDS	123..6431
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607503.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607503.1

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGAA AAACACCTAT TGGGATGAGA AAGAACATGA TTCACCAGGA ACCTCAGAGC 
AGCGGCCTCA GTTCCAGACT GACAGGACTT CAGGAGGATG TAGGAAAATC ACCTGGTAGG 
AGGAGGTCTG CTAGCATCAG CACACTTCCA CTTGACCGCT CTGGGTCATC AATGGTACCT 
TCCTACGACA GCTCCGTCAG CCCCCCTACC AGCAGAGCCA TGTCGGTGTC AAAGGCTGGG 
ACAAAATTAG ATCACAGTGA GGAGGAACGT AAAATACTAC TGGACTCCTC CCAGTTGAAG 
GACTTATGGA GGAAAATCTG TCATAATGGA ACAGGGATGG AATTCCAGGA CCACAGATAC 
TGGCTGAGAA CCTACCCCAA CTGCATTGTG GGAAAAGAGC TCGTCAACTG GCTGCTAAGA 
AATGGCACCA TTTCCAACAG GGTCCAAGCT ATTGCCATAG GTCAGGCACT CGTGGATGGC 
CGCTGGCTCG ACTGTGTCAC CCACCACGAT CAGCTATTCA GAGATGAGTA TGCTCTCTAT 
CGACCACTTC AGAGTACAGA GTTCTCAGAA ACTCCCTCCC CGGACAGCGA TAGTGTCAAC 
TCACTAGAGG GACATTCAGA GCCGTCATGG TTCAAGGACA TCAAATTCGA TGACAGCGAC 
ACCGAGCAGC CCGCAGACGA CACAGAGTAC ACCATGCCTA GTTCCGCTGG GCCCAGCAAG 
AGGACATCTG TCAGCAGCGT CCACTCAGCA GTAGACAGTG ACTCAGCTGC CTCCATCAAC 
CTCAACATGG AGCAAAACAA TGTGAATTTT CGAATCAAGA AAGATTCCAA GTGTCCCCAG 
GCATTCCCCG CCCTGGAGCA CAAATCTGAA ATTATGGCGT CTGAAGATGG AGGAGAGAAC 
ATCCTTATAA GTGATGCCTT TATCAAAGAG TCTCTCTTTA ACCGTCGTGT GGAGGAGAAG 
GCTAAAGAAA TGCTGTTTAC TCCACTGGGT TGGCACCACA GCTCTCTGGA CCAGCTCAGA 
GAGGAGAATG GAGAAAAGAA GGCTATGGAG AGGCTGCTCT CGGCAAATCA CAGCCACATG 
ATGGCGCTGC TGCAGCAGTT GCTCTACAGC GAGTCTTTGT CTCTGTCCTG GCGGGATATT 
ATCGTTCCTG TGGTTCGCCA GGTTGTCCAG ACAGTTCGAC CTGATGTTCG CAACAGTGAT 
GACGACATGG ACATCCGCCA ACTGGTCCAT ATCAAAAAGA TTCCTGGAGG AAAAAAGTTT 
GACTCAACTG TAGTGAATGG CTTTGTGTGT ACGAAGAGTA TTGCCCACAA AAAGATGAAC 
CCATATATCA AGAACCCCAA AATTCTGCTG CTGAAATGCT CAATAGAGTA TCTGTACAGG 
GAAGAGACCA AATTCACGTC CATCGACCCC ATCGTGCTGC AGGAACGAGA GTTTCTGAAA 
AACTATGTGC AACGTATCGT GGATGTTCGG CCCACTCTGG TTCTGGTGGA AAAAACGGTG 
TCTCGTATAG CTCAGGACAT GCTGCTAGAA CACGGCATTA CCCTGGTCAT TAATGTCAAA 
CCGCAAGTAT TGGACAGGGT GAGTTGTGTG ACCCAGGGGG ACCTTGTCAT GTCCATGGAC 
CAGCTACTCA CCAAACCTCG TCTGGGTACC TGCCACAAGT TTTACATGCA GCCCTTCCCT 
TTGGCCAATA ATGAGGTCAA GACTCTGATG TTCTTCGAAG GATGTCCTGC TCATCTCGGG 
TGCTCCATAA ATCTACGTGG TGCTTCAGAC TACGAGTTAG CCAGGGTGAA GGAGATCATG 
CGATTAATGG TGTGTGTGGC CTACCACTCT CAGCTGGAGA TCTCCTTCCT CATGGATGAG 
TTTGCCATGC CGCCCAGCTT GTCCCAGAGC ACTTCATTTC CCTGTCTCCT CGACGGAGGC 
TCTGCAGATG AGGAAGCAAG CAGGGAGAAA CTAGAGAAGA GTTCTGAAGA TGAAGACACT 
GTAGTGGAAG GACAACCTGA GGAGGTGCCC TCTGCCAATG ATGTAGACAC TTCCCAGGAC 
ACAAATGATC CTAAATTATT CTCCTCTATG GTGGAAGAGA CCACTAATAC TGACACAGTC 
ACGACAAAGT CTTCCACTCC GTTCCAAAGC CCTCGTGCGC CATGCTTGCC AGTTCCTCCA 
CCATTCATCA TGGAAGAAAC CCCAGAAATG GGCTCAGACA GCCTAACAGG GGCATTTGAG 
GCAGGGGTGG TGGAATGCTG CCCAAAGGAG GAATCAACCA ACCTAGAAGA TGGAGAAGAT 
TCTGATATTC CTACTCCTAA ATTGTTCCGA GACCCCCTGC AAGATGATAC TGGGATGTTT 
GTGGCTGAAG AAGTTGCTTC TTCAGATGAC CATCTCAAGT CCATCTCTGC TATCTTCAAA 
CAGGAACTTA AGGACATCAT CCTGTGCATT TCACCTTTCA TCACATTTGA GAAACCATTT 
CTTCTTACCC CTGCTGGGAT GCACTGCCCG AGCAGGGATT ATTTTCCCGA ACAGGTCTAT 
CTGTCTCCTC TCCTCAACAA GGACTTTAAA GAGTTGGATG GACGCAGAAA GCGGCAGCTC 
CTTAAGGAGT CCACTCCCTC CCTGGCAAAC AGCCTAACGA ATGGTGGCCC CCCGTCAAAG 
CATGTCAGTG TCCTGCCATC CCACAGTCTC ACCAGCACCC GGATCGTTGA ACTAGTAAAC 
AGGAGCCAGG GTCTGGCCAG AATGGTTGCA GACTACAGAG CAAAGGGTGG ACGTATCCAG 
CACAGCGAAG CTACTGATCC ATTTTGCATA AGCAGCTCTT CTGCTCCGGT CAGTGGCCAG 
AATAGACCAG GTGATGGTTC GGTCAGAGCA CCAGTAAAGA CTGACAGCGA AGAGGAGAAG 
CCAACTAAAC CCAGTGACTG GACCCCCAAG CTGGATTGTC TGAACCCCAC CAACCACCAG 
AGACTCTGTG TGCTTTTTAG CAGTTCATCA ACTCAGTCCA GCAATGCCCC CAACCCCTGT 
GTCAACCCAT GGATTGTTAC CATGGAGTTT TATGGCAAGA ATGACCTCTG CCTTGGTGTA 
TTCTTGGAGC GATATTGCTT TAGGCCATCA TACCAGTGCC CCAGTATATT TTGTGAGACC 
CCCATGGTGC ACCACATCCG GCGTTTTGTC CATGGCAACG GCTGCGTGCA GATAGTACTG 
AAGGAGCTGG ACTCCCCTGT GCCGGGGTAC CAGCACACCA TTCTCAACTA CTCCTGGTGC 
CGCATCTGCA AACAAGTGAC ACCAGTGGTT CCTCTATCCA ATGACTCATG GTCAATGTCT 
TTTGCAAAAT ATCTGGAGCT TCGCTTCTAC GGCCACCAGT ACACCAGGAG GGCCAATGCA 
GAGCCCTGTG GCCACTCCAT CCACAAAGAC TACCACCAGT ACTTCTCATA CAACCAAATG 
GTGGCTTCCT TCAGTTACAC CTCAGTGAGG CTGCTGGAGA TTTGTCTTCC TCGTTTCAAA 
ATCTTCATCA GGAATCAGGG TCCTTCGATC AACAACTTGG GGCAGGATCT GAAAGACTTC 
TCTCAAAAAG TAAATCAGGT TTACGTGGCC ATAGATGATC GTCTCACATC TCTAAAGACG 
GACACCTTCA GTAAAACACG CGAGGAGAAG ATGGAGGACC TCTTTGCACA GAAAGATATG 
GAAGAGGCGG AACTGCGCAG CTGGATTGAA AAGTTTCAAG CACGATTGCA GGCTTGTGGT 
CACGTTTCTC CTCAGCAGCT GCAGACGACT CTGGAATCAC TGGTGATGAA GAAACAGAGT 
CTGTGTGAGA TGTTGCAGTA CTGGAACAGC AGGCTGCAGG AATTATTCCA ACAGGAGAAA 
GGCAGGAAAC GTCTGTCAGT GCCCCCAAGC CCCGGCAGGC ACAGACAGAC TGCCCCTGAT 
GATGGCAAGA ACGCTGTGGA GTCATCCCCA CGTAACCCTT CACCTGTGGT ACAGAATGGT 
GAAAAGGAGG ACCGTCACCT CACCACACTG CCTTCAACCT CCTCTCTGTT GCCCTCGCCT 
GGAGACCTGG GAGCTGAACC AGCCACATCT GGTCCTTCCC TGAGTGAGCA GGACTCTGTC 
AGCATCCCAG AAGATGTATT TGATGGACAC TTGCTGGGCT CCAATGACAG TCAGGTGAAG 
GAGAAATCCA CCATGAAAGC CATCCTTGCC AACTTCCTGC CCGGCAACAG TTACAACCCC 
ATTCCATTCC CATTTGACCC AGACAAACAC TACCTGATGG ATGAGTATGA GCGAGTGCCA 
ATTGCAGTGT GTGAAAGAGA ACCAAGCTCC ATCATTGCAT TTGCTCTGAG TTGTAAGAAG 
TATAAAACAG CACTGGAAGA TCTGTCCAAG GTGTCAAACA TGGGTGGAGA AGACTCTTCA 
CAAGCTGCCA GTAGTGGAGA GAGTCGGGTG AGGGGCAGCC CCGCCAGGCC CAGTGAGTCA 
GCCTCGTCCC AACAGAGCCG CAGCAGCAGT GAAGCAGATC CCCTCAAAGA CGCAGACCTA 
ACAGACAAAC AGAAGAAGCA GGCCCTGAAT CCCCACATTG AGCTACAGTT CTCTGATGCA 
AGCGCAAAGT TTTACTGTCG GATCTACTAC GCCGAGGAGT TTCATAAGAT GCGTGAGGAG 
ATCATGGAGA GCTCCGAAGA GGACTTTGTG CGTTCCCTGT CGCACTGTGT CAACTGGCAG 
GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC TACGCAACAG AAGACGACCG TTTCATCCTA 
AAACAGATGC CCAGGCTGGA GGTGCAGTCC TTCTTGGACT TTGCTCCGCA CTACTTCACC 
TACATCACCG GAGCTGTGCA GCAGAAGAGG CCAACTGCAT TGGCAAAGAT TCTCGGAGTT 
TACAGGATAG GCTACAAGAA CTCCCAGAAC AACACTGAGA AGAAACTGGA CCTGCTCGTC 
ATGGAAAACC TGTTCTACGG ACGCAAGATG GCTCAGGTCT TTGACCTGAA AGGTTCCCTC 
AGAAACCGTC ATGTGAAGAC AGACCTGGTA AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT 
GAGAATCTAC TGAAGCTGAT CCACGACAAT CCGCTCTACA TTCGCTCTCA CTGCAAGTCC 
ATCTTGCGCG CCGCCATACA CAGCGACGCC TACTTCCTGT CCAGCCACCT CATCATTGAC 
TACTCCCTGC TGGTGGGGTG GGACGATGCC ACCGACCAGC TGGTGGTTGG GATCATAGAC 
TATATTCGGA CGTTTACCTG GGACAAGAAA TTAGAGATGG TTGTCAAATC CACTGGAATC 
CTGGGGGGTC AGGGAAAGAT GCCCACCGTT GTTTCTCCTG AGCTGTACCG AGCTCGGTTC 
TGTGAGGCCA TGGACAAATA CTTCCTGATG GTACCCGACC ACTGGACGGG TCTTGGCATC 
AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607509.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605811.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.

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CloneID	OTo31328
Clone ID Related Accession (Same CDS sequence)	XM_011607509.1
Accession Version	XM_011607509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGCA TGATTCACCA GGAACCTCAG AGCAGCGGCC TCAGTTCCAG ACTGACAGGA 
CTTCAGGAGG ATGTAGGAAA ATCACCTGGT AGGAGGAGGT CTGCTAGCAT CAGCACACTT 
CCACTTGACC GCTCTGGGTC ATCAATGGTA CCTTCCTACG ACAGCTCCGT CAGCCCCCCT 
ACCAGCAGAG CCATGTCGGT GTCAAAGGCT GGGACAAAAT TAGATCACAG TGAGGAGGAA 
CGTAAAATAC TACTGGACTC CTCCCAGTTG AAGGACTTAT GGAGGAAAAT CTGTCATAAT 
GGAACAGGGA TGGAATTCCA GGACCACAGA TACTGGCTGA GAACCTACCC CAACTGCATT 
GTGGGAAAAG AGCTCGTCAA CTGGCTGCTA AGAAATGGCA CCATTTCCAA CAGGGTCCAA 
GCTATTGCCA TAGGTCAGGC ACTCGTGGAT GGCCGCTGGC TCGACTGTGT CACCCACCAC 
GATCAGCTAT TCAGAGATGA GTATGCTCTC TATCGACCAC TTCAGAGTAC AGAGTTCTCA 
GAAACTCCCT CCCCGGACAG CGATAGTGTC AACTCACTAG AGGGACATTC AGAGCCGTCA 
TGGTTCAAGG ACATCAAATT CGATGACAGC GACACCGAGC AGCCCGCAGA CGACACAGAG 
TACACCATGC CTAGTTCCGC TGGGCCCAGC AAGAGGACAT CTGTCAGCAG CGTCCACTCA 
GCAGTAGACA GTGACTCAGC TGCCTCCATC AACCTCAACA TGGAGCAAAA CAATGTGAAT 
TTTCGAATCA AGAAAGATTC CAAGTGTCCC CAGGCATTCC CCGCCCTGGA GCACAAATCT 
GAAATTATGG CGTCTGAAGA TGGAGGAGAG AACATCCTTA TAAGTGATGC CTTTATCAAA 
GAGTCTCTCT TTAACCGTCG TGTGGAGGAG AAGGCTAAAG AAATGCTGTT TACTCCACTG 
GGTTGGCACC ACAGCTCTCT GGACCAGCTC AGAGAGGAGA ATGGAGAAAA GAAGGCTATG 
GAGAGGCTGC TCTCGGCAAA TCACAGCCAC ATGATGGCGC TGCTGCAGCA GTTGCTCTAC 
AGCGAGTCTT TGTCTCTGTC CTGGCGGGAT ATTATCGTTC CTGTGGTTCG CCAGGTTGTC 
CAGACAGTTC GACCTGATGT TCGCAACAGT GATGACGACA TGGACATCCG CCAACTGGTC 
CATATCAAAA AGATTCCTGG AGGAAAAAAG TTTGACTCAA CTGTAGTGAA TGGCTTTGTG 
TGTACGAAGA GTATTGCCCA CAAAAAGATG AACCCATATA TCAAGAACCC CAAAATTCTG 
CTGCTGAAAT GCTCAATAGA GTATCTGTAC AGGGAAGAGA CCAAATTCAC GTCCATCGAC 
CCCATCGTGC TGCAGGAACG AGAGTTTCTG AAAAACTATG TGCAACGTAT CGTGGATGTT 
CGGCCCACTC TGGTTCTGGT GGAAAAAACG GTGTCTCGTA TAGCTCAGGA CATGCTGCTA 
GAACACGGCA TTACCCTGGT CATTAATGTC AAACCGCAAG TATTGGACAG GGTGAGTTGT 
GTGACCCAGG GGGACCTTGT CATGTCCATG GACCAGCTAC TCACCAAACC TCGTCTGGGT 
ACCTGCCACA AGTTTTACAT GCAGCCCTTC CCTTTGGCCA ATAATGAGGT CAAGACTCTG 
ATGTTCTTCG AAGGATGTCC TGCTCATCTC GGGTGCTCCA TAAATCTACG TGGTGCTTCA 
GACTACGAGT TAGCCAGGGT GAAGGAGATC ATGCGATTAA TGGTGTGTGT GGCCTACCAC 
TCTCAGCTGG AGATCTCCTT CCTCATGGAT GAGTTTGCCA TGCCGCCCAG CTTGTCCCAG 
AGCACTTCAT TTCCCTGTCT CCTCGACGGA GGCTCTGCAG ATGAGGAAGC AAGCAGGGAG 
AAACTAGAGA AGAGTTCTGA AGATGAAGAC ACTGTAGTGG AAGGACAACC TGAGGAGGTG 
CCCTCTGCCA ATGATGTAGA CACTTCCCAG GACACAAATG ATCCTAAATT ATTCTCCTCT 
ATGGTGGAAG AGACCACTAA TACTGACACA GTCACGACAA AGTCTTCCAC TCCGTTCCAA 
AGCCCTCGTG CGCCATGCTT GCCAGTTCCT CCACCATTCA TCATGGAAGA AACCCCAGAA 
ATGGGCTCAG ACAGCCTAAC AGGGGCATTT GAGGCAGGGG TGGTGGAATG CTGCCCAAAG 
GAGGAATCAA CCAACCTAGA AGATGGAGAA GATTCTGATA TTCCTACTCC TAAATTGTTC 
CGAGACCCCC TGCAAGATGA TACTGGGATG TTTGTGGCTG AAGAAGTTGC TTCTTCAGAT 
GACCATCTCA AGTCCATCTC TGCTATCTTC AAACAGGAAC TTAAGGACAT CATCCTGTGC 
ATTTCACCTT TCATCACATT TGAGAAACCA TTTCTTCTTA CCCCTGCTGG GATGCACTGC 
CCGAGCAGGG ATTATTTTCC CGAACAGGTC TATCTGTCTC CTCTCCTCAA CAAGGACTTT 
AAAGAGTTGG ATGGACGCAG AAAGCGGCAG CTCCTTAAGG AGTCCACTCC CTCCCTGGCA 
AACAGCCTAA CGAATGGTGG CCCCCCGTCA AAGCATGTCA GTGTCCTGCC ATCCCACAGT 
CTCACCAGCA CCCGGATCGT TGAACTAGTA AACAGGAGCC AGGGTCTGGC CAGAATGGTT 
GCAGACTACA GAGCAAAGGG TGGACGTATC CAGCACAGCG AAGCTACTGA TCCATTTTGC 
ATAAGCAGCT CTTCTGCTCC GGTCAGTGGC CAGAATAGAC CAGGTGATGG TTCGGTCAGA 
GCACCAGTAA AGACTGACAG CGAAGAGGAG AAGCCAACTA AACCCAGTGA CTGGACCCCC 
AAGCTGGATT GTCTGAACCC CACCAACCAC CAGAGACTCT GTGTGCTTTT TAGCAGTTCA 
TCAACTCAGT CCAGCAATGC CCCCAACCCC TGTGTCAACC CATGGATTGT TACCATGGAG 
TTTTATGGCA AGAATGACCT CTGCCTTGGT GTATTCTTGG AGCGATATTG CTTTAGGCCA 
TCATACCAGT GCCCCAGTAT ATTTTGTGAG ACCCCCATGG TGCACCACAT CCGGCGTTTT 
GTCCATGGCA ACGGCTGCGT GCAGATAGTA CTGAAGGAGC TGGACTCCCC TGTGCCGGGG 
TACCAGCACA CCATTCTCAA CTACTCCTGG TGCCGCATCT GCAAACAAGT GACACCAGTG 
GTTCCTCTAT CCAATGACTC ATGGTCAATG TCTTTTGCAA AATATCTGGA GCTTCGCTTC 
TACGGCCACC AGTACACCAG GAGGGCCAAT GCAGAGCCCT GTGGCCACTC CATCCACAAA 
GACTACCACC AGTACTTCTC ATACAACCAA ATGGTGGCTT CCTTCAGTTA CACCTCAGTG 
AGGCTGCTGG AGATTTGTCT TCCTCGTTTC AAAATCTTCA TCAGGAATCA GGGTCCTTCG 
ATCAACAACT TGGGGCAGGA TCTGAAAGAC TTCTCTCAAA AAGTAAATCA GGTTTACGTG 
GCCATAGATG ATCGTCTCAC ATCTCTAAAG ACGGACACCT TCAGTAAAAC ACGCGAGGAG 
AAGATGGAGG ACCTCTTTGC ACAGAAAGAT ATGGAAGAGG CGGAACTGCG CAGCTGGATT 
GAAAAGTTTC AAGCACGATT GCAGGCTTGT GGTCACGTTT CTCCTCAGCA GCTGCAGACG 
ACTCTGGAAT CACTGGTGAT GAAGAAACAG AGTCTGTGTG AGATGTTGCA GTACTGGAAC 
AGCAGGCTGC AGGAATTATT CCAACAGGAG AAAGGCAGGA AACGTCTGTC AGTGCCCCCA 
AGCCCCGGCA GGCACAGACA GACTGCCCCT GATGATGGCA AGAACGCTGT GGAGTCATCC 
CCACGTAACC CTTCACCTGT GGTACAGAAT GGTGAAAAGG AGGACCGTCA CCTCACCACA 
CTGCCTTCAA CCTCCTCTCT GTTGCCCTCG CCTGGAGACC TGGGAGCTGA ACCAGCCACA 
TCTGGTCCTT CCCTGAGTGA GCAGGACTCT GTCAGCATCC CAGAAGATGT ATTTGATGGA 
CACTTGCTGG GCTCCAATGA CAGTCAGGTG AAGGAGAAAT CCACCATGAA AGCCATCCTT 
GCCAACTTCC TGCCCGGCAA CAGTTACAAC CCCATTCCAT TCCCATTTGA CCCAGACAAA 
CACTACCTGA TGGATGAGTA TGAGCGAGTG CCAATTGCAG TGTGTGAAAG AGAACCAAGC 
TCCATCATTG CATTTGCTCT GAGTTGTAAG AAGTATAAAA CAGCACTGGA AGATCTGTCC 
AAGGTGTCAA ACATGGGTGG AGAAGACTCT TCACAAGCTG CCAGTAGTGG AGAGAGTCGG 
GTGAGGGGCA GCCCCGCCAG GCCCAGTGAG TCAGCCTCGT CCCAACAGAG CCGCAGCAGC 
AGTGAAGCAG ATCCCCTCAG TAGGCGTCCA GAAGACGCAG ACCTAACAGA CAAACAGAAG 
AAGCAGGCCC TGAATCCCCA CATTGAGCTA CAGTTCTCTG ATGCAAGCGC AAAGTTTTAC 
TGTCGGATCT ACTACGCCGA GGAGTTTCAT AAGATGCGTG AGGAGATCAT GGAGAGCTCC 
GAAGAGGACT TTGTGCGTTC CCTGTCGCAC TGTGTCAACT GGCAGGCGCG CGGTGGAAAG 
TCTGGGGCCG TTTTCTACGC AACAGAAGAC GACCGTTTCA TCCTAAAACA GATGCCCAGG 
CTGGAGGTGC AGTCCTTCTT GGACTTTGCT CCGCACTACT TCACCTACAT CACCGGAGCT 
GTGCAGCAGA AGAGGCCAAC TGCATTGGCA AAGATTCTCG GAGTTTACAG GATAGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAA CTGGACCTGC TCGTCATGGA AAACCTGTTC 
TACGGACGCA AGATGGCTCA GGTCTTTGAC CTGAAAGGTT CCCTCAGAAA CCGTCATGTG 
AAGACAGACC TGGTAAAGGA AAGCTGCGAG GTGGTCCTGC TGGATGAGAA TCTACTGAAG 
CTGATCCACG ACAATCCGCT CTACATTCGC TCTCACTGCA AGTCCATCTT GCGCGCCGCC 
ATACACAGCG ACGCCTACTT CCTGTCCAGC CACCTCATCA TTGACTACTC CCTGCTGGTG 
GGGTGGGACG ATGCCACCGA CCAGCTGGTG GTTGGGATCA TAGACTATAT TCGGACGTTT 
ACCTGGGACA AGAAATTAGA GATGGTTGTC AAATCCACTG GAATCCTGGG GGGTCAGGGA 
AAGATGCCCA CCGTTGTTTC TCCTGAGCTG TACCGAGCTC GGTTCTGTGA GGCCATGGAC 
AAATACTTCC TGATGGTACC CGACCACTGG ACGGGTCTTG GCATCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605811.1
CDS	123..6416
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQSMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607509.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607509.1

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGCA TGATTCACCA GGAACCTCAG AGCAGCGGCC TCAGTTCCAG ACTGACAGGA 
CTTCAGGAGG ATGTAGGAAA ATCACCTGGT AGGAGGAGGT CTGCTAGCAT CAGCACACTT 
CCACTTGACC GCTCTGGGTC ATCAATGGTA CCTTCCTACG ACAGCTCCGT CAGCCCCCCT 
ACCAGCAGAG CCATGTCGGT GTCAAAGGCT GGGACAAAAT TAGATCACAG TGAGGAGGAA 
CGTAAAATAC TACTGGACTC CTCCCAGTTG AAGGACTTAT GGAGGAAAAT CTGTCATAAT 
GGAACAGGGA TGGAATTCCA GGACCACAGA TACTGGCTGA GAACCTACCC CAACTGCATT 
GTGGGAAAAG AGCTCGTCAA CTGGCTGCTA AGAAATGGCA CCATTTCCAA CAGGGTCCAA 
GCTATTGCCA TAGGTCAGGC ACTCGTGGAT GGCCGCTGGC TCGACTGTGT CACCCACCAC 
GATCAGCTAT TCAGAGATGA GTATGCTCTC TATCGACCAC TTCAGAGTAC AGAGTTCTCA 
GAAACTCCCT CCCCGGACAG CGATAGTGTC AACTCACTAG AGGGACATTC AGAGCCGTCA 
TGGTTCAAGG ACATCAAATT CGATGACAGC GACACCGAGC AGCCCGCAGA CGACACAGAG 
TACACCATGC CTAGTTCCGC TGGGCCCAGC AAGAGGACAT CTGTCAGCAG CGTCCACTCA 
GCAGTAGACA GTGACTCAGC TGCCTCCATC AACCTCAACA TGGAGCAAAA CAATGTGAAT 
TTTCGAATCA AGAAAGATTC CAAGTGTCCC CAGGCATTCC CCGCCCTGGA GCACAAATCT 
GAAATTATGG CGTCTGAAGA TGGAGGAGAG AACATCCTTA TAAGTGATGC CTTTATCAAA 
GAGTCTCTCT TTAACCGTCG TGTGGAGGAG AAGGCTAAAG AAATGCTGTT TACTCCACTG 
GGTTGGCACC ACAGCTCTCT GGACCAGCTC AGAGAGGAGA ATGGAGAAAA GAAGGCTATG 
GAGAGGCTGC TCTCGGCAAA TCACAGCCAC ATGATGGCGC TGCTGCAGCA GTTGCTCTAC 
AGCGAGTCTT TGTCTCTGTC CTGGCGGGAT ATTATCGTTC CTGTGGTTCG CCAGGTTGTC 
CAGACAGTTC GACCTGATGT TCGCAACAGT GATGACGACA TGGACATCCG CCAACTGGTC 
CATATCAAAA AGATTCCTGG AGGAAAAAAG TTTGACTCAA CTGTAGTGAA TGGCTTTGTG 
TGTACGAAGA GTATTGCCCA CAAAAAGATG AACCCATATA TCAAGAACCC CAAAATTCTG 
CTGCTGAAAT GCTCAATAGA GTATCTGTAC AGGGAAGAGA CCAAATTCAC GTCCATCGAC 
CCCATCGTGC TGCAGGAACG AGAGTTTCTG AAAAACTATG TGCAACGTAT CGTGGATGTT 
CGGCCCACTC TGGTTCTGGT GGAAAAAACG GTGTCTCGTA TAGCTCAGGA CATGCTGCTA 
GAACACGGCA TTACCCTGGT CATTAATGTC AAACCGCAAG TATTGGACAG GGTGAGTTGT 
GTGACCCAGG GGGACCTTGT CATGTCCATG GACCAGCTAC TCACCAAACC TCGTCTGGGT 
ACCTGCCACA AGTTTTACAT GCAGCCCTTC CCTTTGGCCA ATAATGAGGT CAAGACTCTG 
ATGTTCTTCG AAGGATGTCC TGCTCATCTC GGGTGCTCCA TAAATCTACG TGGTGCTTCA 
GACTACGAGT TAGCCAGGGT GAAGGAGATC ATGCGATTAA TGGTGTGTGT GGCCTACCAC 
TCTCAGCTGG AGATCTCCTT CCTCATGGAT GAGTTTGCCA TGCCGCCCAG CTTGTCCCAG 
AGCACTTCAT TTCCCTGTCT CCTCGACGGA GGCTCTGCAG ATGAGGAAGC AAGCAGGGAG 
AAACTAGAGA AGAGTTCTGA AGATGAAGAC ACTGTAGTGG AAGGACAACC TGAGGAGGTG 
CCCTCTGCCA ATGATGTAGA CACTTCCCAG GACACAAATG ATCCTAAATT ATTCTCCTCT 
ATGGTGGAAG AGACCACTAA TACTGACACA GTCACGACAA AGTCTTCCAC TCCGTTCCAA 
AGCCCTCGTG CGCCATGCTT GCCAGTTCCT CCACCATTCA TCATGGAAGA AACCCCAGAA 
ATGGGCTCAG ACAGCCTAAC AGGGGCATTT GAGGCAGGGG TGGTGGAATG CTGCCCAAAG 
GAGGAATCAA CCAACCTAGA AGATGGAGAA GATTCTGATA TTCCTACTCC TAAATTGTTC 
CGAGACCCCC TGCAAGATGA TACTGGGATG TTTGTGGCTG AAGAAGTTGC TTCTTCAGAT 
GACCATCTCA AGTCCATCTC TGCTATCTTC AAACAGGAAC TTAAGGACAT CATCCTGTGC 
ATTTCACCTT TCATCACATT TGAGAAACCA TTTCTTCTTA CCCCTGCTGG GATGCACTGC 
CCGAGCAGGG ATTATTTTCC CGAACAGGTC TATCTGTCTC CTCTCCTCAA CAAGGACTTT 
AAAGAGTTGG ATGGACGCAG AAAGCGGCAG CTCCTTAAGG AGTCCACTCC CTCCCTGGCA 
AACAGCCTAA CGAATGGTGG CCCCCCGTCA AAGCATGTCA GTGTCCTGCC ATCCCACAGT 
CTCACCAGCA CCCGGATCGT TGAACTAGTA AACAGGAGCC AGGGTCTGGC CAGAATGGTT 
GCAGACTACA GAGCAAAGGG TGGACGTATC CAGCACAGCG AAGCTACTGA TCCATTTTGC 
ATAAGCAGCT CTTCTGCTCC GGTCAGTGGC CAGAATAGAC CAGGTGATGG TTCGGTCAGA 
GCACCAGTAA AGACTGACAG CGAAGAGGAG AAGCCAACTA AACCCAGTGA CTGGACCCCC 
AAGCTGGATT GTCTGAACCC CACCAACCAC CAGAGACTCT GTGTGCTTTT TAGCAGTTCA 
TCAACTCAGT CCAGCAATGC CCCCAACCCC TGTGTCAACC CATGGATTGT TACCATGGAG 
TTTTATGGCA AGAATGACCT CTGCCTTGGT GTATTCTTGG AGCGATATTG CTTTAGGCCA 
TCATACCAGT GCCCCAGTAT ATTTTGTGAG ACCCCCATGG TGCACCACAT CCGGCGTTTT 
GTCCATGGCA ACGGCTGCGT GCAGATAGTA CTGAAGGAGC TGGACTCCCC TGTGCCGGGG 
TACCAGCACA CCATTCTCAA CTACTCCTGG TGCCGCATCT GCAAACAAGT GACACCAGTG 
GTTCCTCTAT CCAATGACTC ATGGTCAATG TCTTTTGCAA AATATCTGGA GCTTCGCTTC 
TACGGCCACC AGTACACCAG GAGGGCCAAT GCAGAGCCCT GTGGCCACTC CATCCACAAA 
GACTACCACC AGTACTTCTC ATACAACCAA ATGGTGGCTT CCTTCAGTTA CACCTCAGTG 
AGGCTGCTGG AGATTTGTCT TCCTCGTTTC AAAATCTTCA TCAGGAATCA GGGTCCTTCG 
ATCAACAACT TGGGGCAGGA TCTGAAAGAC TTCTCTCAAA AAGTAAATCA GGTTTACGTG 
GCCATAGATG ATCGTCTCAC ATCTCTAAAG ACGGACACCT TCAGTAAAAC ACGCGAGGAG 
AAGATGGAGG ACCTCTTTGC ACAGAAAGAT ATGGAAGAGG CGGAACTGCG CAGCTGGATT 
GAAAAGTTTC AAGCACGATT GCAGGCTTGT GGTCACGTTT CTCCTCAGCA GCTGCAGACG 
ACTCTGGAAT CACTGGTGAT GAAGAAACAG AGTCTGTGTG AGATGTTGCA GTACTGGAAC 
AGCAGGCTGC AGGAATTATT CCAACAGGAG AAAGGCAGGA AACGTCTGTC AGTGCCCCCA 
AGCCCCGGCA GGCACAGACA GACTGCCCCT GATGATGGCA AGAACGCTGT GGAGTCATCC 
CCACGTAACC CTTCACCTGT GGTACAGAAT GGTGAAAAGG AGGACCGTCA CCTCACCACA 
CTGCCTTCAA CCTCCTCTCT GTTGCCCTCG CCTGGAGACC TGGGAGCTGA ACCAGCCACA 
TCTGGTCCTT CCCTGAGTGA GCAGGACTCT GTCAGCATCC CAGAAGATGT ATTTGATGGA 
CACTTGCTGG GCTCCAATGA CAGTCAGGTG AAGGAGAAAT CCACCATGAA AGCCATCCTT 
GCCAACTTCC TGCCCGGCAA CAGTTACAAC CCCATTCCAT TCCCATTTGA CCCAGACAAA 
CACTACCTGA TGGATGAGTA TGAGCGAGTG CCAATTGCAG TGTGTGAAAG AGAACCAAGC 
TCCATCATTG CATTTGCTCT GAGTTGTAAG AAGTATAAAA CAGCACTGGA AGATCTGTCC 
AAGGTGTCAA ACATGGGTGG AGAAGACTCT TCACAAGCTG CCAGTAGTGG AGAGAGTCGG 
GTGAGGGGCA GCCCCGCCAG GCCCAGTGAG TCAGCCTCGT CCCAACAGAG CCGCAGCAGC 
AGTGAAGCAG ATCCCCTCAG TAGGCGTCCA GAAGACGCAG ACCTAACAGA CAAACAGAAG 
AAGCAGGCCC TGAATCCCCA CATTGAGCTA CAGTTCTCTG ATGCAAGCGC AAAGTTTTAC 
TGTCGGATCT ACTACGCCGA GGAGTTTCAT AAGATGCGTG AGGAGATCAT GGAGAGCTCC 
GAAGAGGACT TTGTGCGTTC CCTGTCGCAC TGTGTCAACT GGCAGGCGCG CGGTGGAAAG 
TCTGGGGCCG TTTTCTACGC AACAGAAGAC GACCGTTTCA TCCTAAAACA GATGCCCAGG 
CTGGAGGTGC AGTCCTTCTT GGACTTTGCT CCGCACTACT TCACCTACAT CACCGGAGCT 
GTGCAGCAGA AGAGGCCAAC TGCATTGGCA AAGATTCTCG GAGTTTACAG GATAGGCTAC 
AAGAACTCCC AGAACAACAC TGAGAAGAAA CTGGACCTGC TCGTCATGGA AAACCTGTTC 
TACGGACGCA AGATGGCTCA GGTCTTTGAC CTGAAAGGTT CCCTCAGAAA CCGTCATGTG 
AAGACAGACC TGGTAAAGGA AAGCTGCGAG GTGGTCCTGC TGGATGAGAA TCTACTGAAG 
CTGATCCACG ACAATCCGCT CTACATTCGC TCTCACTGCA AGTCCATCTT GCGCGCCGCC 
ATACACAGCG ACGCCTACTT CCTGTCCAGC CACCTCATCA TTGACTACTC CCTGCTGGTG 
GGGTGGGACG ATGCCACCGA CCAGCTGGTG GTTGGGATCA TAGACTATAT TCGGACGTTT 
ACCTGGGACA AGAAATTAGA GATGGTTGTC AAATCCACTG GAATCCTGGG GGGTCAGGGA 
AAGATGCCCA CCGTTGTTTC TCCTGAGCTG TACCGAGCTC GGTTCTGTGA GGCCATGGAC 
AAATACTTCC TGATGGTACC CGACCACTGG ACGGGTCTTG GCATCAACTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607513.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605815.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.

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CloneID	OTo31329
Clone ID Related Accession (Same CDS sequence)	XM_011607513.1
Accession Version	XM_011607513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGCA TGATTCACCA GGAACCTCAG AGCAGCGGCC TCAGTTCCAG ACTGACAGGA 
CTTCAGGAGG ATGTAGGAAA ATCACCTGGT AGGAGGAGGT CTGCTAGCAT CAGCACACTT 
CCACTTGACC GCTCTGGGTC ATCAATGGTA CCTTCCTACG ACAGCTCCGT CAGCCCCCCT 
ACCAGCAGAG CCATGTCGGT GTCAAAGGCT GGGACAAAAT TAGATCACAG TGAGGAGGAA 
CGTAAAATAC TACTGGACTC CTCCCAGTTG AAGGACTTAT GGAGGAAAAT CTGTCATAAT 
GGAACAGGGA TGGAATTCCA GGACCACAGA TACTGGCTGA GAACCTACCC CAACTGCATT 
GTGGGAAAAG AGCTCGTCAA CTGGCTGCTA AGAAATGGCA CCATTTCCAA CAGGGTCCAA 
GCTATTGCCA TAGGTCAGGC ACTCGTGGAT GGCCGCTGGC TCGACTGTGT CACCCACCAC 
GATCAGCTAT TCAGAGATGA GTATGCTCTC TATCGACCAC TTCAGAGTAC AGAGTTCTCA 
GAAACTCCCT CCCCGGACAG CGATAGTGTC AACTCACTAG AGGGACATTC AGAGCCGTCA 
TGGTTCAAGG ACATCAAATT CGATGACAGC GACACCGAGC AGCCCGCAGA CGACACAGAG 
TACACCATGC CTAGTTCCGC TGGGCCCAGC AAGAGGACAT CTGTCAGCAG CGTCCACTCA 
GCAGTAGACA GTGACTCAGC TGCCTCCATC AACCTCAACA TGGAGCAAAA CAATGTGAAT 
TTTCGAATCA AGAAAGATTC CAAGTGTCCC CAGGCATTCC CCGCCCTGGA GCACAAATCT 
GAAATTATGG CGTCTGAAGA TGGAGGAGAG AACATCCTTA TAAGTGATGC CTTTATCAAA 
GAGTCTCTCT TTAACCGTCG TGTGGAGGAG AAGGCTAAAG AAATGCTGTT TACTCCACTG 
GGTTGGCACC ACAGCTCTCT GGACCAGCTC AGAGAGGAGA ATGGAGAAAA GAAGGCTATG 
GAGAGGCTGC TCTCGGCAAA TCACAGCCAC ATGATGGCGC TGCTGCAGCA GTTGCTCTAC 
AGCGAGTCTT TGTCTCTGTC CTGGCGGGAT ATTATCGTTC CTGTGGTTCG CCAGGTTGTC 
CAGACAGTTC GACCTGATGT TCGCAACAGT GATGACGACA TGGACATCCG CCAACTGGTC 
CATATCAAAA AGATTCCTGG AGGAAAAAAG TTTGACTCAA CTGTAGTGAA TGGCTTTGTG 
TGTACGAAGA GTATTGCCCA CAAAAAGATG AACCCATATA TCAAGAACCC CAAAATTCTG 
CTGCTGAAAT GCTCAATAGA GTATCTGTAC AGGGAAGAGA CCAAATTCAC GTCCATCGAC 
CCCATCGTGC TGCAGGAACG AGAGTTTCTG AAAAACTATG TGCAACGTAT CGTGGATGTT 
CGGCCCACTC TGGTTCTGGT GGAAAAAACG GTGTCTCGTA TAGCTCAGGA CATGCTGCTA 
GAACACGGCA TTACCCTGGT CATTAATGTC AAACCGCAAG TATTGGACAG GGTGAGTTGT 
GTGACCCAGG GGGACCTTGT CATGTCCATG GACCAGCTAC TCACCAAACC TCGTCTGGGT 
ACCTGCCACA AGTTTTACAT GCAGCCCTTC CCTTTGGCCA ATAATGAGGT CAAGACTCTG 
ATGTTCTTCG AAGGATGTCC TGCTCATCTC GGGTGCTCCA TAAATCTACG TGGTGCTTCA 
GACTACGAGT TAGCCAGGGT GAAGGAGATC ATGCGATTAA TGGTGTGTGT GGCCTACCAC 
TCTCAGCTGG AGATCTCCTT CCTCATGGAT GAGTTTGCCA TGCCGCCCAG CTTGTCCCAG 
AGCACTTCAT TTCCCTGTCT CCTCGACGGA GGCTCTGCAG ATGAGGAAGC AAGCAGGGAG 
AAACTAGAGA AGAGTTCTGA AGATGAAGAC ACTGTAGTGG AAGGACAACC TGAGGAGGTG 
CCCTCTGCCA ATGATGTAGA CACTTCCCAG GACACAAATG ATCCTAAATT ATTCTCCTCT 
ATGGTGGAAG AGACCACTAA TACTGACACA GTCACGACAA AGTCTTCCAC TCCGTTCCAA 
AGCCCTCGTG CGCCATGCTT GCCAGTTCCT CCACCATTCA TCATGGAAGA AACCCCAGAA 
ATGGGCTCAG ACAGCCTAAC AGGGGCATTT GAGGCAGGGG TGGTGGAATG CTGCCCAAAG 
GAGGAATCAA CCAACCTAGA AGATGGAGAA GATTCTGATA TTCCTACTCC TAAATTGTTC 
CGAGACCCCC TGCAAGATGA TACTGGGATG TTTGTGGCTG AAGAAGTTGC TTCTTCAGAT 
GACCATCTCA AGTCCATCTC TGCTATCTTC AAACAGGAAC TTAAGGACAT CATCCTGTGC 
ATTTCACCTT TCATCACATT TGAGAAACCA TTTCTTCTTA CCCCTGCTGG GATGCACTGC 
CCGAGCAGGG ATTATTTTCC CGAACAGGTC TATCTGTCTC CTCTCCTCAA CAAGGACTTT 
AAAGAGTTGG ATGGACGCAG AAAGCGGCAG CTCCTTAAGG AGTCCACTCC CTCCCTGGCA 
AACAGCCTAA CGAATGGTGG CCCCCCGTCA AAGCATGTCA GTGTCCTGCC ATCCCACAGT 
CTCACCAGCA CCCGGATCGT TGAACTAGTA AACAGGAGCC AGGGTCTGGC CAGAATGGTT 
GCAGACTACA GAGCAAAGGG TGGACGTATC CAGCACAGCG AAGCTACTGA TCCATTTTGC 
ATAAGCAGCT CTTCTGCTCC GGTCAGTGGC CAGAATAGAC CAGGTGATGG TTCGGTCAGA 
GCACCAGTAA AGACTGACAG CGAAGAGGAG AAGCCAACTA AACCCAGTGA CTGGACCCCC 
AAGCTGGATT GTCTGAACCC CACCAACCAC CAGAGACTCT GTGTGCTTTT TAGCAGTTCA 
TCAACTCAGT CCAGCAATGC CCCCAACCCC TGTGTCAACC CATGGATTGT TACCATGGAG 
TTTTATGGCA AGAATGACCT CTGCCTTGGT GTATTCTTGG AGCGATATTG CTTTAGGCCA 
TCATACCAGT GCCCCAGTAT ATTTTGTGAG ACCCCCATGG TGCACCACAT CCGGCGTTTT 
GTCCATGGCA ACGGCTGCGT GCAGATAGTA CTGAAGGAGC TGGACTCCCC TGTGCCGGGG 
TACCAGCACA CCATTCTCAA CTACTCCTGG TGCCGCATCT GCAAACAAGT GACACCAGTG 
GTTCCTCTAT CCAATGACTC ATGGTCAATG TCTTTTGCAA AATATCTGGA GCTTCGCTTC 
TACGGCCACC AGTACACCAG GAGGGCCAAT GCAGAGCCCT GTGGCCACTC CATCCACAAA 
GACTACCACC AGTACTTCTC ATACAACCAA ATGGTGGCTT CCTTCAGTTA CACCTCAGTG 
AGGCTGCTGG AGATTTGTCT TCCTCGTTTC AAAATCTTCA TCAGGAATCA GGGTCCTTCG 
ATCAACAACT TGGGGCAGGA TCTGAAAGAC TTCTCTCAAA AAGTAAATCA GGTTTACGTG 
GCCATAGATG ATCGTCTCAC ATCTCTAAAG ACGGACACCT TCAGTAAAAC ACGCGAGGAG 
AAGATGGAGG ACCTCTTTGC ACAGAAAGAT ATGGAAGAGG CGGAACTGCG CAGCTGGATT 
GAAAAGTTTC AAGCACGATT GCAGGCTTGT GGTCACGTTT CTCCTCAGCA GCTGCAGACG 
ACTCTGGAAT CACTGGTGAT GAAGAAACAG AGTCTGTGTG AGATGTTGCA GTACTGGAAC 
AGCAGGCTGC AGGAATTATT CCAACAGGAG AAAGGCAGGA AACGTCTGTC AGTGCCCCCA 
AGCCCCGGCA GGCACAGACA GACTGCCCCT GATGATGGCA AGAACGCTGT GGAGTCATCC 
CCACGTAACC CTTCACCTGT GGTACAGAAT GGTGAAAAGG AGGACCGTCA CCTCACCACA 
CTGCCTTCAA CCTCCTCTCT GTTGCCCTCG CCTGGAGACC TGGGAGCTGA ACCAGCCACA 
TCTGGTCCTT CCCTGAGTGA GCAGGACTCT GTCAGCATCC CAGAAGATGT ATTTGATGGA 
CACTTGCTGG GCTCCAATGA CAGTCAGGTG AAGGAGAAAT CCACCATGAA AGCCATCCTT 
GCCAACTTCC TGCCCGGCAA CAGTTACAAC CCCATTCCAT TCCCATTTGA CCCAGACAAA 
CACTACCTGA TGGATGAGTA TGAGCGAGTG CCAATTGCAG TGTGTGAAAG AGAACCAAGC 
TCCATCATTG CATTTGCTCT GAGTTGTAAG AAGTATAAAA CAGCACTGGA AGATCTGTCC 
AAGGTGTCAA ACATGGGTGG AGAAGACTCT TCACAAGCTG CCAGTAGTGG AGAGAGTCGG 
GTGAGGGGCA GCCCCGCCAG GCCCAGTGAG TCAGCCTCGT CCCAACAGAG CCGCAGCAGC 
AGTGAAGCAG ATCCCCTCAA AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605815.1
CDS	123..6404
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKVTGSDHSLVKSGDRKGLSGLMPGTKEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQSMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLKDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607513.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607513.1

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GTCACAGGCA GTGACCACAG TCTTGTGAAG TCAGGTGATA GAAAGGGCCT GTCTGGATTG 
ATGCCAGGAA CCAAGGAGGG ACGTTCTCTT TCAACAGTGT CAGAGAAATC AGACACTCCA 
TCCCCTTCCC CTCTATCAGA GAGGAGGAGC TGGTCCTCCA GCCCACCCCA TTCCCTCTAC 
ACTGCCAGGA TTCCCCGAAA ACAACCAGAT CACCTTCAAA GGACCTCTGC TGCTTCAGAT 
GGCAGCAGGA AATCAGAGAC TCCTTTAAGC AATCACGACC CTCGAACTGC TGTGCAACTT 
CGCACTGCAT TGAAGAGGCT GAAGGAAATA ATGGAGGGAA AGAGCCAGGA CAGTGACTTG 
AAGCAGTACT GGATGCCGGA CAGCCAGTGT AAAGAATGTT ATGACTGCAA TGAAAAGTTC 
ACAACCTTCC GACGCCGCCA CCATTGCCGA CTGTGTGGTC AGATTTTCTG CAGTCGCTGC 
TGCAACCAGG AAATCCCTGG AAAGTTCATG GGTTACACAG GAGATTTGCG GGCCTGTACG 
TACTGCCGTA AGTTGGCACT GAGCTACGCC CACTCGGCTG ATTCGGGCTC CATCGGCGAA 
GACTTGAACA CCCTGACTGA CTCTCCGAGC TCTGTGTGTG TGCTGGAGCC CAGTGAGCCA 
CGGACGCCTG TGGGGGGGCG CAAGGCCAGC AGGAACATCT TCCTAGAGGA AGACTTAACA 
TGGCAGAGCA TGATTCACCA GGAACCTCAG AGCAGCGGCC TCAGTTCCAG ACTGACAGGA 
CTTCAGGAGG ATGTAGGAAA ATCACCTGGT AGGAGGAGGT CTGCTAGCAT CAGCACACTT 
CCACTTGACC GCTCTGGGTC ATCAATGGTA CCTTCCTACG ACAGCTCCGT CAGCCCCCCT 
ACCAGCAGAG CCATGTCGGT GTCAAAGGCT GGGACAAAAT TAGATCACAG TGAGGAGGAA 
CGTAAAATAC TACTGGACTC CTCCCAGTTG AAGGACTTAT GGAGGAAAAT CTGTCATAAT 
GGAACAGGGA TGGAATTCCA GGACCACAGA TACTGGCTGA GAACCTACCC CAACTGCATT 
GTGGGAAAAG AGCTCGTCAA CTGGCTGCTA AGAAATGGCA CCATTTCCAA CAGGGTCCAA 
GCTATTGCCA TAGGTCAGGC ACTCGTGGAT GGCCGCTGGC TCGACTGTGT CACCCACCAC 
GATCAGCTAT TCAGAGATGA GTATGCTCTC TATCGACCAC TTCAGAGTAC AGAGTTCTCA 
GAAACTCCCT CCCCGGACAG CGATAGTGTC AACTCACTAG AGGGACATTC AGAGCCGTCA 
TGGTTCAAGG ACATCAAATT CGATGACAGC GACACCGAGC AGCCCGCAGA CGACACAGAG 
TACACCATGC CTAGTTCCGC TGGGCCCAGC AAGAGGACAT CTGTCAGCAG CGTCCACTCA 
GCAGTAGACA GTGACTCAGC TGCCTCCATC AACCTCAACA TGGAGCAAAA CAATGTGAAT 
TTTCGAATCA AGAAAGATTC CAAGTGTCCC CAGGCATTCC CCGCCCTGGA GCACAAATCT 
GAAATTATGG CGTCTGAAGA TGGAGGAGAG AACATCCTTA TAAGTGATGC CTTTATCAAA 
GAGTCTCTCT TTAACCGTCG TGTGGAGGAG AAGGCTAAAG AAATGCTGTT TACTCCACTG 
GGTTGGCACC ACAGCTCTCT GGACCAGCTC AGAGAGGAGA ATGGAGAAAA GAAGGCTATG 
GAGAGGCTGC TCTCGGCAAA TCACAGCCAC ATGATGGCGC TGCTGCAGCA GTTGCTCTAC 
AGCGAGTCTT TGTCTCTGTC CTGGCGGGAT ATTATCGTTC CTGTGGTTCG CCAGGTTGTC 
CAGACAGTTC GACCTGATGT TCGCAACAGT GATGACGACA TGGACATCCG CCAACTGGTC 
CATATCAAAA AGATTCCTGG AGGAAAAAAG TTTGACTCAA CTGTAGTGAA TGGCTTTGTG 
TGTACGAAGA GTATTGCCCA CAAAAAGATG AACCCATATA TCAAGAACCC CAAAATTCTG 
CTGCTGAAAT GCTCAATAGA GTATCTGTAC AGGGAAGAGA CCAAATTCAC GTCCATCGAC 
CCCATCGTGC TGCAGGAACG AGAGTTTCTG AAAAACTATG TGCAACGTAT CGTGGATGTT 
CGGCCCACTC TGGTTCTGGT GGAAAAAACG GTGTCTCGTA TAGCTCAGGA CATGCTGCTA 
GAACACGGCA TTACCCTGGT CATTAATGTC AAACCGCAAG TATTGGACAG GGTGAGTTGT 
GTGACCCAGG GGGACCTTGT CATGTCCATG GACCAGCTAC TCACCAAACC TCGTCTGGGT 
ACCTGCCACA AGTTTTACAT GCAGCCCTTC CCTTTGGCCA ATAATGAGGT CAAGACTCTG 
ATGTTCTTCG AAGGATGTCC TGCTCATCTC GGGTGCTCCA TAAATCTACG TGGTGCTTCA 
GACTACGAGT TAGCCAGGGT GAAGGAGATC ATGCGATTAA TGGTGTGTGT GGCCTACCAC 
TCTCAGCTGG AGATCTCCTT CCTCATGGAT GAGTTTGCCA TGCCGCCCAG CTTGTCCCAG 
AGCACTTCAT TTCCCTGTCT CCTCGACGGA GGCTCTGCAG ATGAGGAAGC AAGCAGGGAG 
AAACTAGAGA AGAGTTCTGA AGATGAAGAC ACTGTAGTGG AAGGACAACC TGAGGAGGTG 
CCCTCTGCCA ATGATGTAGA CACTTCCCAG GACACAAATG ATCCTAAATT ATTCTCCTCT 
ATGGTGGAAG AGACCACTAA TACTGACACA GTCACGACAA AGTCTTCCAC TCCGTTCCAA 
AGCCCTCGTG CGCCATGCTT GCCAGTTCCT CCACCATTCA TCATGGAAGA AACCCCAGAA 
ATGGGCTCAG ACAGCCTAAC AGGGGCATTT GAGGCAGGGG TGGTGGAATG CTGCCCAAAG 
GAGGAATCAA CCAACCTAGA AGATGGAGAA GATTCTGATA TTCCTACTCC TAAATTGTTC 
CGAGACCCCC TGCAAGATGA TACTGGGATG TTTGTGGCTG AAGAAGTTGC TTCTTCAGAT 
GACCATCTCA AGTCCATCTC TGCTATCTTC AAACAGGAAC TTAAGGACAT CATCCTGTGC 
ATTTCACCTT TCATCACATT TGAGAAACCA TTTCTTCTTA CCCCTGCTGG GATGCACTGC 
CCGAGCAGGG ATTATTTTCC CGAACAGGTC TATCTGTCTC CTCTCCTCAA CAAGGACTTT 
AAAGAGTTGG ATGGACGCAG AAAGCGGCAG CTCCTTAAGG AGTCCACTCC CTCCCTGGCA 
AACAGCCTAA CGAATGGTGG CCCCCCGTCA AAGCATGTCA GTGTCCTGCC ATCCCACAGT 
CTCACCAGCA CCCGGATCGT TGAACTAGTA AACAGGAGCC AGGGTCTGGC CAGAATGGTT 
GCAGACTACA GAGCAAAGGG TGGACGTATC CAGCACAGCG AAGCTACTGA TCCATTTTGC 
ATAAGCAGCT CTTCTGCTCC GGTCAGTGGC CAGAATAGAC CAGGTGATGG TTCGGTCAGA 
GCACCAGTAA AGACTGACAG CGAAGAGGAG AAGCCAACTA AACCCAGTGA CTGGACCCCC 
AAGCTGGATT GTCTGAACCC CACCAACCAC CAGAGACTCT GTGTGCTTTT TAGCAGTTCA 
TCAACTCAGT CCAGCAATGC CCCCAACCCC TGTGTCAACC CATGGATTGT TACCATGGAG 
TTTTATGGCA AGAATGACCT CTGCCTTGGT GTATTCTTGG AGCGATATTG CTTTAGGCCA 
TCATACCAGT GCCCCAGTAT ATTTTGTGAG ACCCCCATGG TGCACCACAT CCGGCGTTTT 
GTCCATGGCA ACGGCTGCGT GCAGATAGTA CTGAAGGAGC TGGACTCCCC TGTGCCGGGG 
TACCAGCACA CCATTCTCAA CTACTCCTGG TGCCGCATCT GCAAACAAGT GACACCAGTG 
GTTCCTCTAT CCAATGACTC ATGGTCAATG TCTTTTGCAA AATATCTGGA GCTTCGCTTC 
TACGGCCACC AGTACACCAG GAGGGCCAAT GCAGAGCCCT GTGGCCACTC CATCCACAAA 
GACTACCACC AGTACTTCTC ATACAACCAA ATGGTGGCTT CCTTCAGTTA CACCTCAGTG 
AGGCTGCTGG AGATTTGTCT TCCTCGTTTC AAAATCTTCA TCAGGAATCA GGGTCCTTCG 
ATCAACAACT TGGGGCAGGA TCTGAAAGAC TTCTCTCAAA AAGTAAATCA GGTTTACGTG 
GCCATAGATG ATCGTCTCAC ATCTCTAAAG ACGGACACCT TCAGTAAAAC ACGCGAGGAG 
AAGATGGAGG ACCTCTTTGC ACAGAAAGAT ATGGAAGAGG CGGAACTGCG CAGCTGGATT 
GAAAAGTTTC AAGCACGATT GCAGGCTTGT GGTCACGTTT CTCCTCAGCA GCTGCAGACG 
ACTCTGGAAT CACTGGTGAT GAAGAAACAG AGTCTGTGTG AGATGTTGCA GTACTGGAAC 
AGCAGGCTGC AGGAATTATT CCAACAGGAG AAAGGCAGGA AACGTCTGTC AGTGCCCCCA 
AGCCCCGGCA GGCACAGACA GACTGCCCCT GATGATGGCA AGAACGCTGT GGAGTCATCC 
CCACGTAACC CTTCACCTGT GGTACAGAAT GGTGAAAAGG AGGACCGTCA CCTCACCACA 
CTGCCTTCAA CCTCCTCTCT GTTGCCCTCG CCTGGAGACC TGGGAGCTGA ACCAGCCACA 
TCTGGTCCTT CCCTGAGTGA GCAGGACTCT GTCAGCATCC CAGAAGATGT ATTTGATGGA 
CACTTGCTGG GCTCCAATGA CAGTCAGGTG AAGGAGAAAT CCACCATGAA AGCCATCCTT 
GCCAACTTCC TGCCCGGCAA CAGTTACAAC CCCATTCCAT TCCCATTTGA CCCAGACAAA 
CACTACCTGA TGGATGAGTA TGAGCGAGTG CCAATTGCAG TGTGTGAAAG AGAACCAAGC 
TCCATCATTG CATTTGCTCT GAGTTGTAAG AAGTATAAAA CAGCACTGGA AGATCTGTCC 
AAGGTGTCAA ACATGGGTGG AGAAGACTCT TCACAAGCTG CCAGTAGTGG AGAGAGTCGG 
GTGAGGGGCA GCCCCGCCAG GCCCAGTGAG TCAGCCTCGT CCCAACAGAG CCGCAGCAGC 
AGTGAAGCAG ATCCCCTCAA AGACGCAGAC CTAACAGACA AACAGAAGAA GCAGGCCCTG 
AATCCCCACA TTGAGCTACA GTTCTCTGAT GCAAGCGCAA AGTTTTACTG TCGGATCTAC 
TACGCCGAGG AGTTTCATAA GATGCGTGAG GAGATCATGG AGAGCTCCGA AGAGGACTTT 
GTGCGTTCCC TGTCGCACTG TGTCAACTGG CAGGCGCGCG GTGGAAAGTC TGGGGCCGTT 
TTCTACGCAA CAGAAGACGA CCGTTTCATC CTAAAACAGA TGCCCAGGCT GGAGGTGCAG 
TCCTTCTTGG ACTTTGCTCC GCACTACTTC ACCTACATCA CCGGAGCTGT GCAGCAGAAG 
AGGCCAACTG CATTGGCAAA GATTCTCGGA GTTTACAGGA TAGGCTACAA GAACTCCCAG 
AACAACACTG AGAAGAAACT GGACCTGCTC GTCATGGAAA ACCTGTTCTA CGGACGCAAG 
ATGGCTCAGG TCTTTGACCT GAAAGGTTCC CTCAGAAACC GTCATGTGAA GACAGACCTG 
GTAAAGGAAA GCTGCGAGGT GGTCCTGCTG GATGAGAATC TACTGAAGCT GATCCACGAC 
AATCCGCTCT ACATTCGCTC TCACTGCAAG TCCATCTTGC GCGCCGCCAT ACACAGCGAC 
GCCTACTTCC TGTCCAGCCA CCTCATCATT GACTACTCCC TGCTGGTGGG GTGGGACGAT 
GCCACCGACC AGCTGGTGGT TGGGATCATA GACTATATTC GGACGTTTAC CTGGGACAAG 
AAATTAGAGA TGGTTGTCAA ATCCACTGGA ATCCTGGGGG GTCAGGGAAA GATGCCCACC 
GTTGTTTCTC CTGAGCTGTA CCGAGCTCGG TTCTGTGAGG CCATGGACAA ATACTTCCTG 
ATGGTACCCG ACCACTGGAC GGGTCTTGGC ATCAACTGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pikfyve ( XM_011607520.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011605822.1 Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.

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CloneID	OTo31330
Clone ID Related Accession (Same CDS sequence)	XM_011607520.1
Accession Version	XM_011607520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	1-phosphatidylinositol 3-phosphate 5-kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070903.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GAGGAGGGAC GTTCTCTTTC AACAGTGTCA GAGAAATCAG ACACTCCATC CCCTTCCCCT 
CTATCAGAGA GGAGGAGCTG GTCCTCCAGC CCACCCCATT CCCTCTACAC TGCCAGGATT 
CCCCGAAAAC AACCAGATCA CCTTCAAAGG ACCTCTGCTG CTTCAGATGG CAGCAGGAAA 
TCAGAGACTC CTTTAAGCAA TCACGACCCT CGAACTGCTG TGCAACTTCG CACTGCATTG 
AAGAGGCTGA AGGAAATAAT GGAGGGAAAG AGCCAGGACA GTGACTTGAA GCAGTACTGG 
ATGCCGGACA GCCAGTGTAA AGAATGTTAT GACTGCAATG AAAAGTTCAC AACCTTCCGA 
CGCCGCCACC ATTGCCGACT GTGTGGTCAG ATTTTCTGCA GTCGCTGCTG CAACCAGGAA 
ATCCCTGGAA AGTTCATGGG TTACACAGGA GATTTGCGGG CCTGTACGTA CTGCCGTAAG 
TTGGCACTGA GCTACGCCCA CTCGGCTGAT TCGGGCTCCA TCGGCGAAGA CTTGAACACC 
CTGACTGACT CTCCGAGCTC TGTGTGTGTG CTGGAGCCCA GTGAGCCACG GACGCCTGTG 
GGGGGGCGCA AGGCCAGCAG GAACATCTTC CTAGAGGAAG ACTTAACATG GCAGAGAAAA 
ACACCTATTG GGATGAGAAA GAACATGATT CACCAGGAAC CTCAGAGCAG CGGCCTCAGT 
TCCAGACTGA CAGGACTTCA GGAGGATGTA GGAAAATCAC CTGGTAGGAG GAGGTCTGCT 
AGCATCAGCA CACTTCCACT TGACCGCTCT GGGTCATCAA TGGTACCTTC CTACGACAGC 
TCCGTCAGCC CCCCTACCAG CAGAGCCATG TCGGTGTCAA AGGCTGGGAC AAAATTAGAT 
CACAGTGAGG AGGAACGTAA AATACTACTG GACTCCTCCC AGTTGAAGGA CTTATGGAGG 
AAAATCTGTC ATAATGGAAC AGGGATGGAA TTCCAGGACC ACAGATACTG GCTGAGAACC 
TACCCCAACT GCATTGTGGG AAAAGAGCTC GTCAACTGGC TGCTAAGAAA TGGCACCATT 
TCCAACAGGG TCCAAGCTAT TGCCATAGGT CAGGCACTCG TGGATGGCCG CTGGCTCGAC 
TGTGTCACCC ACCACGATCA GCTATTCAGA GATGAGTATG CTCTCTATCG ACCACTTCAG 
AGTACAGAGT TCTCAGAAAC TCCCTCCCCG GACAGCGATA GTGTCAACTC ACTAGAGGGA 
CATTCAGAGC CGTCATGGTT CAAGGACATC AAATTCGATG ACAGCGACAC CGAGCAGCCC 
GCAGACGACA CAGAGTACAC CATGCCTAGT TCCGCTGGGC CCAGCAAGAG GACATCTGTC 
AGCAGCGTCC ACTCAGCAGT AGACAGTGAC TCAGCTGCCT CCATCAACCT CAACATGGAG 
CAAAACAATG TGAATTTTCG AATCAAGAAA GATTCCAAGT GTCCCCAGGC ATTCCCCGCC 
CTGGAGCACA AATCTGAAAT TATGGCGTCT GAAGATGGAG GAGAGAACAT CCTTATAAGT 
GATGCCTTTA TCAAAGAGTC TCTCTTTAAC CGTCGTGTGG AGGAGAAGGC TAAAGAAATG 
CTGTTTACTC CACTGGGTTG GCACCACAGC TCTCTGGACC AGCTCAGAGA GGAGAATGGA 
GAAAAGAAGG CTATGGAGAG GCTGCTCTCG GCAAATCACA GCCACATGAT GGCGCTGCTG 
CAGCAGTTGC TCTACAGCGA GTCTTTGTCT CTGTCCTGGC GGGATATTAT CGTTCCTGTG 
GTTCGCCAGG TTGTCCAGAC AGTTCGACCT GATGTTCGCA ACAGTGATGA CGACATGGAC 
ATCCGCCAAC TGGTCCATAT CAAAAAGATT CCTGGAGGAA AAAAGTTTGA CTCAACTGTA 
GTGAATGGCT TTGTGTGTAC GAAGAGTATT GCCCACAAAA AGATGAACCC ATATATCAAG 
AACCCCAAAA TTCTGCTGCT GAAATGCTCA ATAGAGTATC TGTACAGGGA AGAGACCAAA 
TTCACGTCCA TCGACCCCAT CGTGCTGCAG GAACGAGAGT TTCTGAAAAA CTATGTGCAA 
CGTATCGTGG ATGTTCGGCC CACTCTGGTT CTGGTGGAAA AAACGGTGTC TCGTATAGCT 
CAGGACATGC TGCTAGAACA CGGCATTACC CTGGTCATTA ATGTCAAACC GCAAGTATTG 
GACAGGGTGA GTTGTGTGAC CCAGGGGGAC CTTGTCATGT CCATGGACCA GCTACTCACC 
AAACCTCGTC TGGGTACCTG CCACAAGTTT TACATGCAGC CCTTCCCTTT GGCCAATAAT 
GAGGTCAAGA CTCTGATGTT CTTCGAAGGA TGTCCTGCTC ATCTCGGGTG CTCCATAAAT 
CTACGTGGTG CTTCAGACTA CGAGTTAGCC AGGGTGAAGG AGATCATGCG ATTAATGGTG 
TGTGTGGCCT ACCACTCTCA GCTGGAGATC TCCTTCCTCA TGGATGAGTT TGCCATGCCG 
CCCAGCTTGT CCCAGAGCAC TTCATTTCCC TGTCTCCTCG ACGGAGGCTC TGCAGATGAG 
GAAGCAAGCA GGGAGAAACT AGAGAAGAGT TCTGAAGATG AAGACACTGT AGTGGAAGGA 
CAACCTGAGG AGGTGCCCTC TGCCAATGAT GTAGACACTT CCCAGGACAC AAATGATCCT 
AAATTATTCT CCTCTATGGT GGAAGAGACC ACTAATACTG ACACAGTCAC GACAAAGTCT 
TCCACTCCGT TCCAAAGCCC TCGTGCGCCA TGCTTGCCAG TTCCTCCACC ATTCATCATG 
GAAGAAACCC CAGAAATGGG CTCAGACAGC CTAACAGGGG CATTTGAGGC AGGGGTGGTG 
GAATGCTGCC CAAAGGAGGA ATCAACCAAC CTAGAAGATG GAGAAGATTC TGATATTCCT 
ACTCCTAAAT TGTTCCGAGA CCCCCTGCAA GATGATACTG GGATGTTTGT GGCTGAAGAA 
GTTGCTTCTT CAGATGACCA TCTCAAGTCC ATCTCTGCTA TCTTCAAACA GGAACTTAAG 
GACATCATCC TGTGCATTTC ACCTTTCATC ACATTTGAGA AACCATTTCT TCTTACCCCT 
GCTGGGATGC ACTGCCCGAG CAGGGATTAT TTTCCCGAAC AGGTCTATCT GTCTCCTCTC 
CTCAACAAGG ACTTTAAAGA GTTGGATGGA CGCAGAAAGC GGCAGCTCCT TAAGGAGTCC 
ACTCCCTCCC TGGCAAACAG CCTAACGAAT GGTGGCCCCC CGTCAAAGCA TGTCAGTGTC 
CTGCCATCCC ACAGTCTCAC CAGCACCCGG ATCGTTGAAC TAGTAAACAG GAGCCAGGGT 
CTGGCCAGAA TGGTTGCAGA CTACAGAGCA AAGGGTGGAC GTATCCAGCA CAGCGAAGCT 
ACTGATCCAT TTTGCATAAG CAGCTCTTCT GCTCCGGTCA GTGGCCAGAA TAGACCAGGT 
GATGGTTCGG TCAGAGCACC AGTAAAGACT GACAGCGAAG AGGAGAAGCC AACTAAACCC 
AGTGACTGGA CCCCCAAGCT GGATTGTCTG AACCCCACCA ACCACCAGAG ACTCTGTGTG 
CTTTTTAGCA GTTCATCAAC TCAGTCCAGC AATGCCCCCA ACCCCTGTGT CAACCCATGG 
ATTGTTACCA TGGAGTTTTA TGGCAAGAAT GACCTCTGCC TTGGTGTATT CTTGGAGCGA 
TATTGCTTTA GGCCATCATA CCAGTGCCCC AGTATATTTT GTGAGACCCC CATGGTGCAC 
CACATCCGGC GTTTTGTCCA TGGCAACGGC TGCGTGCAGA TAGTACTGAA GGAGCTGGAC 
TCCCCTGTGC CGGGGTACCA GCACACCATT CTCAACTACT CCTGGTGCCG CATCTGCAAA 
CAAGTGACAC CAGTGGTTCC TCTATCCAAT GACTCATGGT CAATGTCTTT TGCAAAATAT 
CTGGAGCTTC GCTTCTACGG CCACCAGTAC ACCAGGAGGG CCAATGCAGA GCCCTGTGGC 
CACTCCATCC ACAAAGACTA CCACCAGTAC TTCTCATACA ACCAAATGGT GGCTTCCTTC 
AGTTACACCT CAGTGAGGCT GCTGGAGATT TGTCTTCCTC GTTTCAAAAT CTTCATCAGG 
AATCAGGGTC CTTCGATCAA CAACTTGGGG CAGGATCTGA AAGACTTCTC TCAAAAAGTA 
AATCAGGTTT ACGTGGCCAT AGATGATCGT CTCACATCTC TAAAGACGGA CACCTTCAGT 
AAAACACGCG AGGAGAAGAT GGAGGACCTC TTTGCACAGA AAGATATGGA AGAGGCGGAA 
CTGCGCAGCT GGATTGAAAA GTTTCAAGCA CGATTGCAGG CTTGTGGTCA CGTTTCTCCT 
CAGCAGCTGC AGACGACTCT GGAATCACTG GTGATGAAGA AACAGAGTCT GTGTGAGATG 
TTGCAGTACT GGAACAGCAG GCTGCAGGAA TTATTCCAAC AGGAGAAAGG CAGGAAACGT 
CTGTCAGTGC CCCCAAGCCC CGGCAGGCAC AGACAGACTG CCCCTGATGA TGGCAAGAAC 
GCTGTGGAGT CATCCCCACG TAACCCTTCA CCTGTGGTAC AGAATGGTGA AAAGGAGGAC 
CGTCACCTCA CCACACTGCC TTCAACCTCC TCTCTGTTGC CCTCGCCTGG AGACCTGGGA 
GCTGAACCAG CCACATCTGG TCCTTCCCTG AGTGAGCAGG ACTCTGTCAG CATCCCAGAA 
GATGTATTTG ATGGACACTT GCTGGGCTCC AATGACAGTC AGGTGAAGGA GAAATCCACC 
ATGAAAGCCA TCCTTGCCAA CTTCCTGCCC GGCAACAGTT ACAACCCCAT TCCATTCCCA 
TTTGACCCAG ACAAACACTA CCTGATGGAT GAGTATGAGC GAGTGCCAAT TGCAGTGTGT 
GAAAGAGAAC CAAGCTCCAT CATTGCATTT GCTCTGAGTT GTAAGAAGTA TAAAACAGCA 
CTGGAAGATC TGTCCAAGGT GTCAAACATG GGTGGAGAAG ACTCTTCACA AGCTGCCAGT 
AGTGGAGAGA GTCGGGTGAG GGGCAGCCCC GCCAGGCCCA GTGAGTCAGC CTCGTCCCAA 
CAGAGCCGCA GCAGCAGTGA AGCAGATCCC CTCAGTAGGC GTCCAGAAGA CGCAGACCTA 
ACAGACAAAC AGAAGAAGCA GGCCCTGAAT CCCCACATTG AGCTACAGTT CTCTGATGCA 
AGCGCAAAGT TTTACTGTCG GATCTACTAC GCCGAGGAGT TTCATAAGAT GCGTGAGGAG 
ATCATGGAGA GCTCCGAAGA GGACTTTGTG CGTTCCCTGT CGCACTGTGT CAACTGGCAG 
GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC TACGCAACAG AAGACGACCG TTTCATCCTA 
AAACAGATGC CCAGGCTGGA GGTGCAGTCC TTCTTGGACT TTGCTCCGCA CTACTTCACC 
TACATCACCG GAGCTGTGCA GCAGAAGAGG CCAACTGCAT TGGCAAAGAT TCTCGGAGTT 
TACAGGATAG GCTACAAGAA CTCCCAGAAC AACACTGAGA AGAAACTGGA CCTGCTCGTC 
ATGGAAAACC TGTTCTACGG ACGCAAGATG GCTCAGGTCT TTGACCTGAA AGGTTCCCTC 
AGAAACCGTC ATGTGAAGAC AGACCTGGTA AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT 
GAGAATCTAC TGAAGCTGAT CCACGACAAT CCGCTCTACA TTCGCTCTCA CTGCAAGTCC 
ATCTTGCGCG CCGCCATACA CAGCGACGCC TACTTCCTGT CCAGCCACCT CATCATTGAC 
TACTCCCTGC TGGTGGGGTG GGACGATGCC ACCGACCAGC TGGTGGTTGG GATCATAGAC 
TATATTCGGA CGTTTACCTG GGACAAGAAA TTAGAGATGG TTGTCAAATC CACTGGAATC 
CTGGGGGGTC AGGGAAAGAT GCCCACCGTT GTTTCTCCTG AGCTGTACCG AGCTCGGTTC 
TGTGAGGCCA TGGACAAATA CTTCCTGATG GTACCCGACC ACTGGACGGG TCTTGGCATC 
AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605822.1
CDS	123..6371
Translation	MAADDKSSSSSMEWTTETPVTLPVSPSHLTHFKPLSPEQDEPPLRSAYSSFVNLFRFSNKEEGRSLSTVSEKSDTPSPSPLSERRSWSSSPPHSLYTARIPRKQPDHLQRTSAASDGSRKSETPLSNHDPRTAVQLRTALKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCNEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKLALSYAHSADSGSIGEDLNTLTDSPSSVCVLEPSEPRTPVGGRKASRNIFLEEDLTWQRKTPIGMRKNMIHQEPQSSGLSSRLTGLQEDVGKSPGRRRSASISTLPLDRSGSSMVPSYDSSVSPPTSRAMSVSKAGTKLDHSEEERKILLDSSQLKDLWRKICHNGTGMEFQDHRYWLRTYPNCIVGKELVNWLLRNGTISNRVQAIAIGQALVDGRWLDCVTHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSLEGHSEPSWFKDIKFDDSDTEQPADDTEYTMPSSAGPSKRTSVSSVHSAVDSDSAASINLNMEQNNVNFRIKKDSKCPQAFPALEHKSEIMASEDGGENILISDAFIKESLFNRRVEEKAKEMLFTPLGWHHSSLDQLREENGEKKAMERLLSANHSHMMALLQQLLYSESLSLSWRDIIVPVVRQVVQTVRPDVRNSDDDMDIRQLVHIKKIPGGKKFDSTVVNGFVCTKSIAHKKMNPYIKNPKILLLKCSIEYLYREETKFTSIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKPQVLDRVSCVTQGDLVMSMDQLLTKPRLGTCHKFYMQPFPLANNEVKTLMFFEGCPAHLGCSINLRGASDYELARVKEIMRLMVCVAYHSQLEISFLMDEFAMPPSLSQSTSFPCLLDGGSADEEASREKLEKSSEDEDTVVEGQPEEVPSANDVDTSQDTNDPKLFSSMVEETTNTDTVTTKSSTPFQSPRAPCLPVPPPFIMEETPEMGSDSLTGAFEAGVVECCPKEESTNLEDGEDSDIPTPKLFRDPLQDDTGMFVAEEVASSDDHLKSISAIFKQELKDIILCISPFITFEKPFLLTPAGMHCPSRDYFPEQVYLSPLLNKDFKELDGRRKRQLLKESTPSLANSLTNGGPPSKHVSVLPSHSLTSTRIVELVNRSQGLARMVADYRAKGGRIQHSEATDPFCISSSSAPVSGQNRPGDGSVRAPVKTDSEEEKPTKPSDWTPKLDCLNPTNHQRLCVLFSSSSTQSSNAPNPCVNPWIVTMEFYGKNDLCLGVFLERYCFRPSYQCPSIFCETPMVHHIRRFVHGNGCVQIVLKELDSPVPGYQHTILNYSWCRICKQVTPVVPLSNDSWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHKDYHQYFSYNQMVASFSYTSVRLLEICLPRFKIFIRNQGPSINNLGQDLKDFSQKVNQVYVAIDDRLTSLKTDTFSKTREEKMEDLFAQKDMEEAELRSWIEKFQARLQACGHVSPQQLQTTLESLVMKKQSLCEMLQYWNSRLQELFQQEKGRKRLSVPPSPGRHRQTAPDDGKNAVESSPRNPSPVVQNGEKEDRHLTTLPSTSSLLPSPGDLGAEPATSGPSLSEQDSVSIPEDVFDGHLLGSNDSQVKEKSTMKAILANFLPGNSYNPIPFPFDPDKHYLMDEYERVPIAVCEREPSSIIAFALSCKKYKTALEDLSKVSNMGGEDSSQAASSGESRVRGSPARPSESASSQQSRSSSEADPLSRRPEDADLTDKQKKQALNPHIELQFSDASAKFYCRIYYAEEFHKMREEIMESSEEDFVRSLSHCVNWQARGGKSGAVFYATEDDRFILKQMPRLEVQSFLDFAPHYFTYITGAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRHVKTDLVKESCEVVLLDENLLKLIHDNPLYIRSHCKSILRAAIHSDAYFLSSHLIIDYSLLVGWDDATDQLVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRARFCEAMDKYFLMVPDHWTGLGINC

Target ORF information:

RefSeq Version	XM_011607520.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphoinositide kinase, FYVE finger containing (pikfyve), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607520.1

ORF Insert Sequence:

ATGGCTGCTG ATGATAAGTC ATCCTCATCC TCAATGGAGT GGACCACTGA GACCCCCGTC 
ACCTTGCCCG TGAGTCCGTC CCACCTGACT CATTTTAAGC CACTGTCACC CGAGCAGGAT 
GAGCCCCCGC TCCGTTCAGC ATACAGCTCA TTTGTCAACC TATTTCGCTT TAGTAACAAA 
GAGGAGGGAC GTTCTCTTTC AACAGTGTCA GAGAAATCAG ACACTCCATC CCCTTCCCCT 
CTATCAGAGA GGAGGAGCTG GTCCTCCAGC CCACCCCATT CCCTCTACAC TGCCAGGATT 
CCCCGAAAAC AACCAGATCA CCTTCAAAGG ACCTCTGCTG CTTCAGATGG CAGCAGGAAA 
TCAGAGACTC CTTTAAGCAA TCACGACCCT CGAACTGCTG TGCAACTTCG CACTGCATTG 
AAGAGGCTGA AGGAAATAAT GGAGGGAAAG AGCCAGGACA GTGACTTGAA GCAGTACTGG 
ATGCCGGACA GCCAGTGTAA AGAATGTTAT GACTGCAATG AAAAGTTCAC AACCTTCCGA 
CGCCGCCACC ATTGCCGACT GTGTGGTCAG ATTTTCTGCA GTCGCTGCTG CAACCAGGAA 
ATCCCTGGAA AGTTCATGGG TTACACAGGA GATTTGCGGG CCTGTACGTA CTGCCGTAAG 
TTGGCACTGA GCTACGCCCA CTCGGCTGAT TCGGGCTCCA TCGGCGAAGA CTTGAACACC 
CTGACTGACT CTCCGAGCTC TGTGTGTGTG CTGGAGCCCA GTGAGCCACG GACGCCTGTG 
GGGGGGCGCA AGGCCAGCAG GAACATCTTC CTAGAGGAAG ACTTAACATG GCAGAGAAAA 
ACACCTATTG GGATGAGAAA GAACATGATT CACCAGGAAC CTCAGAGCAG CGGCCTCAGT 
TCCAGACTGA CAGGACTTCA GGAGGATGTA GGAAAATCAC CTGGTAGGAG GAGGTCTGCT 
AGCATCAGCA CACTTCCACT TGACCGCTCT GGGTCATCAA TGGTACCTTC CTACGACAGC 
TCCGTCAGCC CCCCTACCAG CAGAGCCATG TCGGTGTCAA AGGCTGGGAC AAAATTAGAT 
CACAGTGAGG AGGAACGTAA AATACTACTG GACTCCTCCC AGTTGAAGGA CTTATGGAGG 
AAAATCTGTC ATAATGGAAC AGGGATGGAA TTCCAGGACC ACAGATACTG GCTGAGAACC 
TACCCCAACT GCATTGTGGG AAAAGAGCTC GTCAACTGGC TGCTAAGAAA TGGCACCATT 
TCCAACAGGG TCCAAGCTAT TGCCATAGGT CAGGCACTCG TGGATGGCCG CTGGCTCGAC 
TGTGTCACCC ACCACGATCA GCTATTCAGA GATGAGTATG CTCTCTATCG ACCACTTCAG 
AGTACAGAGT TCTCAGAAAC TCCCTCCCCG GACAGCGATA GTGTCAACTC ACTAGAGGGA 
CATTCAGAGC CGTCATGGTT CAAGGACATC AAATTCGATG ACAGCGACAC CGAGCAGCCC 
GCAGACGACA CAGAGTACAC CATGCCTAGT TCCGCTGGGC CCAGCAAGAG GACATCTGTC 
AGCAGCGTCC ACTCAGCAGT AGACAGTGAC TCAGCTGCCT CCATCAACCT CAACATGGAG 
CAAAACAATG TGAATTTTCG AATCAAGAAA GATTCCAAGT GTCCCCAGGC ATTCCCCGCC 
CTGGAGCACA AATCTGAAAT TATGGCGTCT GAAGATGGAG GAGAGAACAT CCTTATAAGT 
GATGCCTTTA TCAAAGAGTC TCTCTTTAAC CGTCGTGTGG AGGAGAAGGC TAAAGAAATG 
CTGTTTACTC CACTGGGTTG GCACCACAGC TCTCTGGACC AGCTCAGAGA GGAGAATGGA 
GAAAAGAAGG CTATGGAGAG GCTGCTCTCG GCAAATCACA GCCACATGAT GGCGCTGCTG 
CAGCAGTTGC TCTACAGCGA GTCTTTGTCT CTGTCCTGGC GGGATATTAT CGTTCCTGTG 
GTTCGCCAGG TTGTCCAGAC AGTTCGACCT GATGTTCGCA ACAGTGATGA CGACATGGAC 
ATCCGCCAAC TGGTCCATAT CAAAAAGATT CCTGGAGGAA AAAAGTTTGA CTCAACTGTA 
GTGAATGGCT TTGTGTGTAC GAAGAGTATT GCCCACAAAA AGATGAACCC ATATATCAAG 
AACCCCAAAA TTCTGCTGCT GAAATGCTCA ATAGAGTATC TGTACAGGGA AGAGACCAAA 
TTCACGTCCA TCGACCCCAT CGTGCTGCAG GAACGAGAGT TTCTGAAAAA CTATGTGCAA 
CGTATCGTGG ATGTTCGGCC CACTCTGGTT CTGGTGGAAA AAACGGTGTC TCGTATAGCT 
CAGGACATGC TGCTAGAACA CGGCATTACC CTGGTCATTA ATGTCAAACC GCAAGTATTG 
GACAGGGTGA GTTGTGTGAC CCAGGGGGAC CTTGTCATGT CCATGGACCA GCTACTCACC 
AAACCTCGTC TGGGTACCTG CCACAAGTTT TACATGCAGC CCTTCCCTTT GGCCAATAAT 
GAGGTCAAGA CTCTGATGTT CTTCGAAGGA TGTCCTGCTC ATCTCGGGTG CTCCATAAAT 
CTACGTGGTG CTTCAGACTA CGAGTTAGCC AGGGTGAAGG AGATCATGCG ATTAATGGTG 
TGTGTGGCCT ACCACTCTCA GCTGGAGATC TCCTTCCTCA TGGATGAGTT TGCCATGCCG 
CCCAGCTTGT CCCAGAGCAC TTCATTTCCC TGTCTCCTCG ACGGAGGCTC TGCAGATGAG 
GAAGCAAGCA GGGAGAAACT AGAGAAGAGT TCTGAAGATG AAGACACTGT AGTGGAAGGA 
CAACCTGAGG AGGTGCCCTC TGCCAATGAT GTAGACACTT CCCAGGACAC AAATGATCCT 
AAATTATTCT CCTCTATGGT GGAAGAGACC ACTAATACTG ACACAGTCAC GACAAAGTCT 
TCCACTCCGT TCCAAAGCCC TCGTGCGCCA TGCTTGCCAG TTCCTCCACC ATTCATCATG 
GAAGAAACCC CAGAAATGGG CTCAGACAGC CTAACAGGGG CATTTGAGGC AGGGGTGGTG 
GAATGCTGCC CAAAGGAGGA ATCAACCAAC CTAGAAGATG GAGAAGATTC TGATATTCCT 
ACTCCTAAAT TGTTCCGAGA CCCCCTGCAA GATGATACTG GGATGTTTGT GGCTGAAGAA 
GTTGCTTCTT CAGATGACCA TCTCAAGTCC ATCTCTGCTA TCTTCAAACA GGAACTTAAG 
GACATCATCC TGTGCATTTC ACCTTTCATC ACATTTGAGA AACCATTTCT TCTTACCCCT 
GCTGGGATGC ACTGCCCGAG CAGGGATTAT TTTCCCGAAC AGGTCTATCT GTCTCCTCTC 
CTCAACAAGG ACTTTAAAGA GTTGGATGGA CGCAGAAAGC GGCAGCTCCT TAAGGAGTCC 
ACTCCCTCCC TGGCAAACAG CCTAACGAAT GGTGGCCCCC CGTCAAAGCA TGTCAGTGTC 
CTGCCATCCC ACAGTCTCAC CAGCACCCGG ATCGTTGAAC TAGTAAACAG GAGCCAGGGT 
CTGGCCAGAA TGGTTGCAGA CTACAGAGCA AAGGGTGGAC GTATCCAGCA CAGCGAAGCT 
ACTGATCCAT TTTGCATAAG CAGCTCTTCT GCTCCGGTCA GTGGCCAGAA TAGACCAGGT 
GATGGTTCGG TCAGAGCACC AGTAAAGACT GACAGCGAAG AGGAGAAGCC AACTAAACCC 
AGTGACTGGA CCCCCAAGCT GGATTGTCTG AACCCCACCA ACCACCAGAG ACTCTGTGTG 
CTTTTTAGCA GTTCATCAAC TCAGTCCAGC AATGCCCCCA ACCCCTGTGT CAACCCATGG 
ATTGTTACCA TGGAGTTTTA TGGCAAGAAT GACCTCTGCC TTGGTGTATT CTTGGAGCGA 
TATTGCTTTA GGCCATCATA CCAGTGCCCC AGTATATTTT GTGAGACCCC CATGGTGCAC 
CACATCCGGC GTTTTGTCCA TGGCAACGGC TGCGTGCAGA TAGTACTGAA GGAGCTGGAC 
TCCCCTGTGC CGGGGTACCA GCACACCATT CTCAACTACT CCTGGTGCCG CATCTGCAAA 
CAAGTGACAC CAGTGGTTCC TCTATCCAAT GACTCATGGT CAATGTCTTT TGCAAAATAT 
CTGGAGCTTC GCTTCTACGG CCACCAGTAC ACCAGGAGGG CCAATGCAGA GCCCTGTGGC 
CACTCCATCC ACAAAGACTA CCACCAGTAC TTCTCATACA ACCAAATGGT GGCTTCCTTC 
AGTTACACCT CAGTGAGGCT GCTGGAGATT TGTCTTCCTC GTTTCAAAAT CTTCATCAGG 
AATCAGGGTC CTTCGATCAA CAACTTGGGG CAGGATCTGA AAGACTTCTC TCAAAAAGTA 
AATCAGGTTT ACGTGGCCAT AGATGATCGT CTCACATCTC TAAAGACGGA CACCTTCAGT 
AAAACACGCG AGGAGAAGAT GGAGGACCTC TTTGCACAGA AAGATATGGA AGAGGCGGAA 
CTGCGCAGCT GGATTGAAAA GTTTCAAGCA CGATTGCAGG CTTGTGGTCA CGTTTCTCCT 
CAGCAGCTGC AGACGACTCT GGAATCACTG GTGATGAAGA AACAGAGTCT GTGTGAGATG 
TTGCAGTACT GGAACAGCAG GCTGCAGGAA TTATTCCAAC AGGAGAAAGG CAGGAAACGT 
CTGTCAGTGC CCCCAAGCCC CGGCAGGCAC AGACAGACTG CCCCTGATGA TGGCAAGAAC 
GCTGTGGAGT CATCCCCACG TAACCCTTCA CCTGTGGTAC AGAATGGTGA AAAGGAGGAC 
CGTCACCTCA CCACACTGCC TTCAACCTCC TCTCTGTTGC CCTCGCCTGG AGACCTGGGA 
GCTGAACCAG CCACATCTGG TCCTTCCCTG AGTGAGCAGG ACTCTGTCAG CATCCCAGAA 
GATGTATTTG ATGGACACTT GCTGGGCTCC AATGACAGTC AGGTGAAGGA GAAATCCACC 
ATGAAAGCCA TCCTTGCCAA CTTCCTGCCC GGCAACAGTT ACAACCCCAT TCCATTCCCA 
TTTGACCCAG ACAAACACTA CCTGATGGAT GAGTATGAGC GAGTGCCAAT TGCAGTGTGT 
GAAAGAGAAC CAAGCTCCAT CATTGCATTT GCTCTGAGTT GTAAGAAGTA TAAAACAGCA 
CTGGAAGATC TGTCCAAGGT GTCAAACATG GGTGGAGAAG ACTCTTCACA AGCTGCCAGT 
AGTGGAGAGA GTCGGGTGAG GGGCAGCCCC GCCAGGCCCA GTGAGTCAGC CTCGTCCCAA 
CAGAGCCGCA GCAGCAGTGA AGCAGATCCC CTCAGTAGGC GTCCAGAAGA CGCAGACCTA 
ACAGACAAAC AGAAGAAGCA GGCCCTGAAT CCCCACATTG AGCTACAGTT CTCTGATGCA 
AGCGCAAAGT TTTACTGTCG GATCTACTAC GCCGAGGAGT TTCATAAGAT GCGTGAGGAG 
ATCATGGAGA GCTCCGAAGA GGACTTTGTG CGTTCCCTGT CGCACTGTGT CAACTGGCAG 
GCGCGCGGTG GAAAGTCTGG GGCCGTTTTC TACGCAACAG AAGACGACCG TTTCATCCTA 
AAACAGATGC CCAGGCTGGA GGTGCAGTCC TTCTTGGACT TTGCTCCGCA CTACTTCACC 
TACATCACCG GAGCTGTGCA GCAGAAGAGG CCAACTGCAT TGGCAAAGAT TCTCGGAGTT 
TACAGGATAG GCTACAAGAA CTCCCAGAAC AACACTGAGA AGAAACTGGA CCTGCTCGTC 
ATGGAAAACC TGTTCTACGG ACGCAAGATG GCTCAGGTCT TTGACCTGAA AGGTTCCCTC 
AGAAACCGTC ATGTGAAGAC AGACCTGGTA AAGGAAAGCT GCGAGGTGGT CCTGCTGGAT 
GAGAATCTAC TGAAGCTGAT CCACGACAAT CCGCTCTACA TTCGCTCTCA CTGCAAGTCC 
ATCTTGCGCG CCGCCATACA CAGCGACGCC TACTTCCTGT CCAGCCACCT CATCATTGAC 
TACTCCCTGC TGGTGGGGTG GGACGATGCC ACCGACCAGC TGGTGGTTGG GATCATAGAC 
TATATTCGGA CGTTTACCTG GGACAAGAAA TTAGAGATGG TTGTCAAATC CACTGGAATC 
CTGGGGGGTC AGGGAAAGAT GCCCACCGTT GTTTCTCCTG AGCTGTACCG AGCTCGGTTC 
TGTGAGGCCA TGGACAAATA CTTCCTGATG GTACCCGACC ACTGGACGGG TCTTGGCATC 
AACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	pikfyve
Entrez Gene ID	101078741
Full Name	phosphoinositide kinase, FYVE-type zinc finger containing
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

pikfyve cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene

Clones

gRNAs

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