col18a1 gene cDNA ORF clone, Takifugu rubripes(torafugu)

mRNA	Protein	Name
XM_011612198.1	XP_011610500.1	collagen alpha-1(XVIII) chain isoform X1
XM_011612206.1	XP_011610508.1	collagen alpha-1(XVIII) chain isoform X3
XM_029833618.1	XP_029689478.1	collagen alpha-1(XVIII) chain isoform X5
XM_011612203.1	XP_011610505.1	collagen alpha-1(XVIII) chain isoform X2
XM_011612206.2	XP_011610508.2	collagen alpha-1(XVIII) chain isoform X4
XM_011612212.2	XP_011610514.2	collagen alpha-1(XVIII) chain isoform X6
XM_029833608.1	XP_029689468.1	collagen alpha-1(XVIII) chain isoform X3
XM_011612198.2	XP_011610500.2	collagen alpha-1(XVIII) chain isoform X1
XM_011612212.1	XP_011610514.1	collagen alpha-1(XVIII) chain isoform X4
XM_011612203.2	XP_011610505.2	collagen alpha-1(XVIII) chain isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo30507	XM_011612198.1 Latest version!	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo30508	XM_011612203.1 Latest version!	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo30509	XM_011612206.1 Latest version!	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo30510	XM_011612212.1 Latest version!	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo41076	XM_011612198.2 Latest version!	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo41077	XM_011612203.2 Latest version!	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo41078	XM_029833608.1 Latest version!	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo41079	XM_011612206.2 Latest version!	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo41080	XM_029833618.1 Latest version!	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo41081	XM_011612212.2 Latest version!	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

col18a1 ( XM_011612198.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610500.1 Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo30507
Clone ID Related Accession (Same CDS sequence)	XM_011612198.1
Accession Version	XM_011612198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XVIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCACC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCGCCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGCAGTCAAA GGGGAGAAAG GAGAGCATGG TGAACAAGGC 
CTTCCAGGAA TCCCTGGAAC AGCCTCTAAT TTTGATATCT ATGCATACAA GAATGAACTG 
AAGGGAGAGC GTGGAGATCA AGGCCTGAAA GGAGAAAAGG GTGAGTCTGG GGGATATTAT 
GACCCACGTT TTGGTGGAGT ACAAGGACCT CCTGGCACAC CAGGCCTGCC TGGCCCCAAA 
GGGGACTCAA TAACTGGCCC TCCTGGACCC CAGGGGCCAC CAGGACAGCC AGGCATTGGT 
TACGATGGAC CTGCAGGTTC TCCAGGACCA CCCGGACCAC CTGGAGCTCC TGGAACTAGC 
ACATACAATT ACCCTGTCAG TCTGCCTGGG CCACCTGGTC CTCCCGGTCC ACCTGGAATT 
CCAGGTCACT CCTCTGGGGT TACAGTTTTA AGGTCATATG ACACTATGAT TGCGACTGCC 
AGACGTCAGC CAGAAGGAAA CCTAATCTAC ATCATCGACA AAGCAGACCT GTACTTGAGA 
GTACGCAATG GACTACGGCA AGTCATGCTT GGAAACTACA GTCCCTTCTT TAGAGAACTG 
GAGAACGAGG TGGCAGAGGC CCAGCCTCCA CCTGTCATCC TGTATCCCCA TTCCCAAGAC 
CAGTCCCACA ACAACGGCGC TGGCCATTAC TCCCAGGGTG GACCTGCTAT ACAACCCATT 
GATCCTCCAC CACAACCGCC TGTAAACAAC AACTACCCTC CCCACTATGA GCCCAGAGTC 
CCTGAAACGA GACACACGGG CCACACAGAA GGACGATCAG GCACTCAGAC AGAGACCCAA 
CACCCCGTGA CCCCACAGCG ACCACCCAAC CCGCCCGTAC TGCAGCCTCT CGGGAATGTT 
GAAACATCAG CATCAGCACT ACATCTCATC GCCTTAAATG CCCCACACAC GGGTAACATG 
CGAGGAATCC GCGGGGCAGA TTTCCTCTGC TTCCAACAGG CCCGAGCCAT CGGACTCAAG 
GGCACCTTCA GAGCATTCCT CTCCTCCAAG CTTCAAGACC TCTACACGAT CGTCCGAAGG 
TCAGACAGGG ACAACTTCCC CATCGTGAAC CTCAAGGACC AAGTGTTGTT CAGAAGCTGG 
GAATCGATGT TTGGTGACGA AGCTAGCAAA ATTACGGAGA ATGTACCCAT CTACTCCTTC 
GATGGCAGAG ATATTCTCAG GGATAGTGCA TGGCCAGAGA AAATGGTCTG GCATGGATCG 
AGCAACAAAG GCCACCGGCA AACAGACCAG TACTGTGAAA CATGGCGAGC GGGAGATCGC 
GCTGTGACTG GTCTGGCATC ATCACTTAAG AGCGGCTACC TCCTGCAGCA GTCCGCCAGC 
AGCTGTTCTG GCTCCTACGT TGTTCTCTGC ATAGAGAACG CCTTCACGTC ACACTCCAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610500.1
CDS	334..4539
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALTFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPAVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612198.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612198.1

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCACC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCGCCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGCAGTCAAA GGGGAGAAAG GAGAGCATGG TGAACAAGGC 
CTTCCAGGAA TCCCTGGAAC AGCCTCTAAT TTTGATATCT ATGCATACAA GAATGAACTG 
AAGGGAGAGC GTGGAGATCA AGGCCTGAAA GGAGAAAAGG GTGAGTCTGG GGGATATTAT 
GACCCACGTT TTGGTGGAGT ACAAGGACCT CCTGGCACAC CAGGCCTGCC TGGCCCCAAA 
GGGGACTCAA TAACTGGCCC TCCTGGACCC CAGGGGCCAC CAGGACAGCC AGGCATTGGT 
TACGATGGAC CTGCAGGTTC TCCAGGACCA CCCGGACCAC CTGGAGCTCC TGGAACTAGC 
ACATACAATT ACCCTGTCAG TCTGCCTGGG CCACCTGGTC CTCCCGGTCC ACCTGGAATT 
CCAGGTCACT CCTCTGGGGT TACAGTTTTA AGGTCATATG ACACTATGAT TGCGACTGCC 
AGACGTCAGC CAGAAGGAAA CCTAATCTAC ATCATCGACA AAGCAGACCT GTACTTGAGA 
GTACGCAATG GACTACGGCA AGTCATGCTT GGAAACTACA GTCCCTTCTT TAGAGAACTG 
GAGAACGAGG TGGCAGAGGC CCAGCCTCCA CCTGTCATCC TGTATCCCCA TTCCCAAGAC 
CAGTCCCACA ACAACGGCGC TGGCCATTAC TCCCAGGGTG GACCTGCTAT ACAACCCATT 
GATCCTCCAC CACAACCGCC TGTAAACAAC AACTACCCTC CCCACTATGA GCCCAGAGTC 
CCTGAAACGA GACACACGGG CCACACAGAA GGACGATCAG GCACTCAGAC AGAGACCCAA 
CACCCCGTGA CCCCACAGCG ACCACCCAAC CCGCCCGTAC TGCAGCCTCT CGGGAATGTT 
GAAACATCAG CATCAGCACT ACATCTCATC GCCTTAAATG CCCCACACAC GGGTAACATG 
CGAGGAATCC GCGGGGCAGA TTTCCTCTGC TTCCAACAGG CCCGAGCCAT CGGACTCAAG 
GGCACCTTCA GAGCATTCCT CTCCTCCAAG CTTCAAGACC TCTACACGAT CGTCCGAAGG 
TCAGACAGGG ACAACTTCCC CATCGTGAAC CTCAAGGACC AAGTGTTGTT CAGAAGCTGG 
GAATCGATGT TTGGTGACGA AGCTAGCAAA ATTACGGAGA ATGTACCCAT CTACTCCTTC 
GATGGCAGAG ATATTCTCAG GGATAGTGCA TGGCCAGAGA AAATGGTCTG GCATGGATCG 
AGCAACAAAG GCCACCGGCA AACAGACCAG TACTGTGAAA CATGGCGAGC GGGAGATCGC 
GCTGTGACTG GTCTGGCATC ATCACTTAAG AGCGGCTACC TCCTGCAGCA GTCCGCCAGC 
AGCTGTTCTG GCTCCTACGT TGTTCTCTGC ATAGAGAACG CCTTCACGTC ACACTCCAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_011612203.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610505.1 Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo30508
Clone ID Related Accession (Same CDS sequence)	XM_011612203.1
Accession Version	XM_011612203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XVIII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCACC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCGCCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGTCAAAGGG GAGAAAGGAG AGCATGGTGA ACAAGGCCTT 
CCAGGAATCC CTGGAACAGC CTCTAATTTT GATATCTATG CATACAAGAA TGAACTGAAG 
GGAGAGCGTG GAGATCAAGG CCTGAAAGGA GAAAAGGGTG AGTCTGGGGG ATATTATGAC 
CCACGTTTTG GTGGAGTACA AGGACCTCCT GGCACACCAG GCCTGCCTGG CCCCAAAGGG 
GACTCAATAA CTGGCCCTCC TGGACCCCAG GGGCCACCAG GACAGCCAGG CATTGGTTAC 
GATGGACCTG CAGGTTCTCC AGGACCACCC GGACCACCTG GAGCTCCTGG AACTAGCACA 
TACAATTACC CTGTCAGTCT GCCTGGGCCA CCTGGTCCTC CCGGTCCACC TGGAATTCCA 
GGTCACTCCT CTGGGGTTAC AGTTTTAAGG TCATATGACA CTATGATTGC GACTGCCAGA 
CGTCAGCCAG AAGGAAACCT AATCTACATC ATCGACAAAG CAGACCTGTA CTTGAGAGTA 
CGCAATGGAC TACGGCAAGT CATGCTTGGA AACTACAGTC CCTTCTTTAG AGAACTGGAG 
AACGAGGTGG CAGAGGCCCA GCCTCCACCT GTCATCCTGT ATCCCCATTC CCAAGACCAG 
TCCCACAACA ACGGCGCTGG CCATTACTCC CAGGGTGGAC CTGCTATACA ACCCATTGAT 
CCTCCACCAC AACCGCCTGT AAACAACAAC TACCCTCCCC ACTATGAGCC CAGAGTCCCT 
GAAACGAGAC ACACGGGCCA CACAGAAGGA CGATCAGGCA CTCAGACAGA GACCCAACAC 
CCCGTGACCC CACAGCGACC ACCCAACCCG CCCGTACTGC AGCCTCTCGG GAATGTTGAA 
ACATCAGCAT CAGCACTACA TCTCATCGCC TTAAATGCCC CACACACGGG TAACATGCGA 
GGAATCCGCG GGGCAGATTT CCTCTGCTTC CAACAGGCCC GAGCCATCGG ACTCAAGGGC 
ACCTTCAGAG CATTCCTCTC CTCCAAGCTT CAAGACCTCT ACACGATCGT CCGAAGGTCA 
GACAGGGACA ACTTCCCCAT CGTGAACCTC AAGGACCAAG TGTTGTTCAG AAGCTGGGAA 
TCGATGTTTG GTGACGAAGC TAGCAAAATT ACGGAGAATG TACCCATCTA CTCCTTCGAT 
GGCAGAGATA TTCTCAGGGA TAGTGCATGG CCAGAGAAAA TGGTCTGGCA TGGATCGAGC 
AACAAAGGCC ACCGGCAAAC AGACCAGTAC TGTGAAACAT GGCGAGCGGG AGATCGCGCT 
GTGACTGGTC TGGCATCATC ACTTAAGAGC GGCTACCTCC TGCAGCAGTC CGCCAGCAGC 
TGTTCTGGCT CCTACGTTGT TCTCTGCATA GAGAACGCCT TCACGTCACA CTCCAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610505.1
CDS	335..4537
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALTFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612203.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612203.1

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCACC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCGCCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGTCAAAGGG GAGAAAGGAG AGCATGGTGA ACAAGGCCTT 
CCAGGAATCC CTGGAACAGC CTCTAATTTT GATATCTATG CATACAAGAA TGAACTGAAG 
GGAGAGCGTG GAGATCAAGG CCTGAAAGGA GAAAAGGGTG AGTCTGGGGG ATATTATGAC 
CCACGTTTTG GTGGAGTACA AGGACCTCCT GGCACACCAG GCCTGCCTGG CCCCAAAGGG 
GACTCAATAA CTGGCCCTCC TGGACCCCAG GGGCCACCAG GACAGCCAGG CATTGGTTAC 
GATGGACCTG CAGGTTCTCC AGGACCACCC GGACCACCTG GAGCTCCTGG AACTAGCACA 
TACAATTACC CTGTCAGTCT GCCTGGGCCA CCTGGTCCTC CCGGTCCACC TGGAATTCCA 
GGTCACTCCT CTGGGGTTAC AGTTTTAAGG TCATATGACA CTATGATTGC GACTGCCAGA 
CGTCAGCCAG AAGGAAACCT AATCTACATC ATCGACAAAG CAGACCTGTA CTTGAGAGTA 
CGCAATGGAC TACGGCAAGT CATGCTTGGA AACTACAGTC CCTTCTTTAG AGAACTGGAG 
AACGAGGTGG CAGAGGCCCA GCCTCCACCT GTCATCCTGT ATCCCCATTC CCAAGACCAG 
TCCCACAACA ACGGCGCTGG CCATTACTCC CAGGGTGGAC CTGCTATACA ACCCATTGAT 
CCTCCACCAC AACCGCCTGT AAACAACAAC TACCCTCCCC ACTATGAGCC CAGAGTCCCT 
GAAACGAGAC ACACGGGCCA CACAGAAGGA CGATCAGGCA CTCAGACAGA GACCCAACAC 
CCCGTGACCC CACAGCGACC ACCCAACCCG CCCGTACTGC AGCCTCTCGG GAATGTTGAA 
ACATCAGCAT CAGCACTACA TCTCATCGCC TTAAATGCCC CACACACGGG TAACATGCGA 
GGAATCCGCG GGGCAGATTT CCTCTGCTTC CAACAGGCCC GAGCCATCGG ACTCAAGGGC 
ACCTTCAGAG CATTCCTCTC CTCCAAGCTT CAAGACCTCT ACACGATCGT CCGAAGGTCA 
GACAGGGACA ACTTCCCCAT CGTGAACCTC AAGGACCAAG TGTTGTTCAG AAGCTGGGAA 
TCGATGTTTG GTGACGAAGC TAGCAAAATT ACGGAGAATG TACCCATCTA CTCCTTCGAT 
GGCAGAGATA TTCTCAGGGA TAGTGCATGG CCAGAGAAAA TGGTCTGGCA TGGATCGAGC 
AACAAAGGCC ACCGGCAAAC AGACCAGTAC TGTGAAACAT GGCGAGCGGG AGATCGCGCT 
GTGACTGGTC TGGCATCATC ACTTAAGAGC GGCTACCTCC TGCAGCAGTC CGCCAGCAGC 
TGTTCTGGCT CCTACGTTGT TCTCTGCATA GAGAACGCCT TCACGTCACA CTCCAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_011612206.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610508.1 Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X3, mRNA.

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CloneID	OTo30509
Clone ID Related Accession (Same CDS sequence)	XM_011612206.1
Accession Version	XM_011612206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XVIII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCACC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCGCCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGACGATAT GGAGGGATCA GGTGGAGGCT TCATCAACAG GCAACCTGGA 
GTCAGAGGAC CAGAGGGAAA ACAGGGTCCT CCGGGCTTCC CCGGACTTCC AGGGAAACCT 
GGCTTCCCTG GTCTACCAGG GCCAAAAGGT AATGATGGTC CTGAGGGGAA AGATGGACGG 
CCAGGGTTGG ATGGCTTCCC TGGACCTCAG GGACCAAAGG GTGACAGAGG CGACAAAGGA 
GACAGGGGTG AAGCGGGGCA GGATGGCACT GGACTACCGG GCCCACCTGG GCCACCTGGC 
CTTCCAGGAG AAATCATCTA CCGGTCATCA GGCAACACTG ACGGTGCTGT CGGCAGCGTG 
GGGCCTCAGG GAATACCTGG ACAACCTGGA TTCCCCGGGC CAGCAGGGCC AAAAGGTGAC 
AGGGGAGAAC CTGGCTTTCC AGGCTTTGGA GAGAAGGGTG AGAAAGGTGA GCCAGGCTTA 
GCGATCGGAC CCGATGGATA TCGTCTGTCT CTGGAGGGAC TAACAGGTCT GAAGGGAGAG 
AGGGGAATAC CTGGACCAGT TGGACCTCCT GGCCCATATG GCCCTCCTGG ATTAAAGGGT 
GAAATTGGAA TGCCAGGCAG ATCTGGTCGT CCTGGTATAA ATGGATACAA GGGTGAGAAA 
GGAGAGCCTG GGACTGGTTC TGGATTTGCC TACCCAGGGC CACCAGGACC ACCTGGACCA 
CCAGGCCCTC CAGGACCAAC CATCAACCAA GATCGTTTCA ATCGCTACGA TGACACACAC 
AGAAATTATC CAGCAGTCAA AGGGGAGAAA GGAGAGCATG GTGAACAAGG CCTTCCAGGA 
ATCCCTGGAA CAGCCTCTAA TTTTGATATC TATGCATACA AGAATGAACT GAAGGGAGAG 
CGTGGAGATC AAGGCCTGAA AGGAGAAAAG GGTGAGTCTG GGGGATATTA TGACCCACGT 
TTTGGTGGAG TACAAGGACC TCCTGGCACA CCAGGCCTGC CTGGCCCCAA AGGGGACTCA 
ATAACTGGCC CTCCTGGACC CCAGGGGCCA CCAGGACAGC CAGGCATTGG TTACGATGGA 
CCTGCAGGTT CTCCAGGACC ACCCGGACCA CCTGGAGCTC CTGGAACTAG CACATACAAT 
TACCCTGTCA GTCTGCCTGG GCCACCTGGT CCTCCCGGTC CACCTGGAAT TCCAGGTCAC 
TCCTCTGGGG TTACAGTTTT AAGGTCATAT GACACTATGA TTGCGACTGC CAGACGTCAG 
CCAGAAGGAA ACCTAATCTA CATCATCGAC AAAGCAGACC TGTACTTGAG AGTACGCAAT 
GGACTACGGC AAGTCATGCT TGGAAACTAC AGTCCCTTCT TTAGAGAACT GGAGAACGAG 
GTGGCAGAGG CCCAGCCTCC ACCTGTCATC CTGTATCCCC ATTCCCAAGA CCAGTCCCAC 
AACAACGGCG CTGGCCATTA CTCCCAGGGT GGACCTGCTA TACAACCCAT TGATCCTCCA 
CCACAACCGC CTGTAAACAA CAACTACCCT CCCCACTATG AGCCCAGAGT CCCTGAAACG 
AGACACACGG GCCACACAGA AGGACGATCA GGCACTCAGA CAGAGACCCA ACACCCCGTG 
ACCCCACAGC GACCACCCAA CCCGCCCGTA CTGCAGCCTC TCGGGAATGT TGAAACATCA 
GCATCAGCAC TACATCTCAT CGCCTTAAAT GCCCCACACA CGGGTAACAT GCGAGGAATC 
CGCGGGGCAG ATTTCCTCTG CTTCCAACAG GCCCGAGCCA TCGGACTCAA GGGCACCTTC 
AGAGCATTCC TCTCCTCCAA GCTTCAAGAC CTCTACACGA TCGTCCGAAG GTCAGACAGG 
GACAACTTCC CCATCGTGAA CCTCAAGGAC CAAGTGTTGT TCAGAAGCTG GGAATCGATG 
TTTGGTGACG AAGCTAGCAA AATTACGGAG AATGTACCCA TCTACTCCTT CGATGGCAGA 
GATATTCTCA GGGATAGTGC ATGGCCAGAG AAAATGGTCT GGCATGGATC GAGCAACAAA 
GGCCACCGGC AAACAGACCA GTACTGTGAA ACATGGCGAG CGGGAGATCG CGCTGTGACT 
GGTCTGGCAT CATCACTTAA GAGCGGCTAC CTCCTGCAGC AGTCCGCCAG CAGCTGTTCT 
GGCTCCTACG TTGTTCTCTG CATAGAGAAC GCCTTCACGT CACACTCCAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610508.1
CDS	335..4531
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALTFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPAVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612206.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612206.1

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCACC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCGCCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGACGATAT GGAGGGATCA GGTGGAGGCT TCATCAACAG GCAACCTGGA 
GTCAGAGGAC CAGAGGGAAA ACAGGGTCCT CCGGGCTTCC CCGGACTTCC AGGGAAACCT 
GGCTTCCCTG GTCTACCAGG GCCAAAAGGT AATGATGGTC CTGAGGGGAA AGATGGACGG 
CCAGGGTTGG ATGGCTTCCC TGGACCTCAG GGACCAAAGG GTGACAGAGG CGACAAAGGA 
GACAGGGGTG AAGCGGGGCA GGATGGCACT GGACTACCGG GCCCACCTGG GCCACCTGGC 
CTTCCAGGAG AAATCATCTA CCGGTCATCA GGCAACACTG ACGGTGCTGT CGGCAGCGTG 
GGGCCTCAGG GAATACCTGG ACAACCTGGA TTCCCCGGGC CAGCAGGGCC AAAAGGTGAC 
AGGGGAGAAC CTGGCTTTCC AGGCTTTGGA GAGAAGGGTG AGAAAGGTGA GCCAGGCTTA 
GCGATCGGAC CCGATGGATA TCGTCTGTCT CTGGAGGGAC TAACAGGTCT GAAGGGAGAG 
AGGGGAATAC CTGGACCAGT TGGACCTCCT GGCCCATATG GCCCTCCTGG ATTAAAGGGT 
GAAATTGGAA TGCCAGGCAG ATCTGGTCGT CCTGGTATAA ATGGATACAA GGGTGAGAAA 
GGAGAGCCTG GGACTGGTTC TGGATTTGCC TACCCAGGGC CACCAGGACC ACCTGGACCA 
CCAGGCCCTC CAGGACCAAC CATCAACCAA GATCGTTTCA ATCGCTACGA TGACACACAC 
AGAAATTATC CAGCAGTCAA AGGGGAGAAA GGAGAGCATG GTGAACAAGG CCTTCCAGGA 
ATCCCTGGAA CAGCCTCTAA TTTTGATATC TATGCATACA AGAATGAACT GAAGGGAGAG 
CGTGGAGATC AAGGCCTGAA AGGAGAAAAG GGTGAGTCTG GGGGATATTA TGACCCACGT 
TTTGGTGGAG TACAAGGACC TCCTGGCACA CCAGGCCTGC CTGGCCCCAA AGGGGACTCA 
ATAACTGGCC CTCCTGGACC CCAGGGGCCA CCAGGACAGC CAGGCATTGG TTACGATGGA 
CCTGCAGGTT CTCCAGGACC ACCCGGACCA CCTGGAGCTC CTGGAACTAG CACATACAAT 
TACCCTGTCA GTCTGCCTGG GCCACCTGGT CCTCCCGGTC CACCTGGAAT TCCAGGTCAC 
TCCTCTGGGG TTACAGTTTT AAGGTCATAT GACACTATGA TTGCGACTGC CAGACGTCAG 
CCAGAAGGAA ACCTAATCTA CATCATCGAC AAAGCAGACC TGTACTTGAG AGTACGCAAT 
GGACTACGGC AAGTCATGCT TGGAAACTAC AGTCCCTTCT TTAGAGAACT GGAGAACGAG 
GTGGCAGAGG CCCAGCCTCC ACCTGTCATC CTGTATCCCC ATTCCCAAGA CCAGTCCCAC 
AACAACGGCG CTGGCCATTA CTCCCAGGGT GGACCTGCTA TACAACCCAT TGATCCTCCA 
CCACAACCGC CTGTAAACAA CAACTACCCT CCCCACTATG AGCCCAGAGT CCCTGAAACG 
AGACACACGG GCCACACAGA AGGACGATCA GGCACTCAGA CAGAGACCCA ACACCCCGTG 
ACCCCACAGC GACCACCCAA CCCGCCCGTA CTGCAGCCTC TCGGGAATGT TGAAACATCA 
GCATCAGCAC TACATCTCAT CGCCTTAAAT GCCCCACACA CGGGTAACAT GCGAGGAATC 
CGCGGGGCAG ATTTCCTCTG CTTCCAACAG GCCCGAGCCA TCGGACTCAA GGGCACCTTC 
AGAGCATTCC TCTCCTCCAA GCTTCAAGAC CTCTACACGA TCGTCCGAAG GTCAGACAGG 
GACAACTTCC CCATCGTGAA CCTCAAGGAC CAAGTGTTGT TCAGAAGCTG GGAATCGATG 
TTTGGTGACG AAGCTAGCAA AATTACGGAG AATGTACCCA TCTACTCCTT CGATGGCAGA 
GATATTCTCA GGGATAGTGC ATGGCCAGAG AAAATGGTCT GGCATGGATC GAGCAACAAA 
GGCCACCGGC AAACAGACCA GTACTGTGAA ACATGGCGAG CGGGAGATCG CGCTGTGACT 
GGTCTGGCAT CATCACTTAA GAGCGGCTAC CTCCTGCAGC AGTCCGCCAG CAGCTGTTCT 
GGCTCCTACG TTGTTCTCTG CATAGAGAAC GCCTTCACGT CACACTCCAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_011612212.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610514.1 Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo30510
Clone ID Related Accession (Same CDS sequence)	XM_011612212.1
Accession Version	XM_011612212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-1(XVIII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070912.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GGTGTGCACG GCTGCAGCGG CTCCTGTGCT GCGTCCTAGT GGTGATAGGT 
CTCGCTGCCT CCCAGTGGCC AGAGGACAAC GGCGTCTCCC TGCTGCAGCT GATCGGGGAT 
CCTCCTCCTC CTGAGATCAC CCAAGTCGAT GGGCCCGAAA ACAGCCCCGC TTACGTCTTT 
GGTCCTGACG CCAACACGGG ACAGTTGGCC CGTGCGCACC TCCCCAGTCC GTTCTACAGA 
GACTTTGCCC TCACCTTCCA CCTGAAGTCC AGCTCTAATA AGGGCGGAGT CGTCTTCTCC 
ATCACAGACG CCTCGCAGCA GATCATGTAC GTCGGTGTTA AACTCTCAGA TGTGCAGGGA 
CGCAACCAGA ACATCATCCT GTTTTACACG GAACCGGGCT CGGAGGAGTC GTACGAGGCG 
GCCAGCTTCC CCGTGCCGTC CACGGTGGAT ACCTGGACTC GCTTTGCCAT CGCCGTGAGG 
GACGACAAGG TAATGTTCTA CTTCAACTGT GAAGTGGACC CTCTGGTGAT GCCCATCGAG 
AGGTCCCCGG ATGAGATGGA GCTGGAGGCC GGAGCTGGGG TCTTCATCGG CCAAGCTGGA 
GGAGCCGATC CTGGAAAGTT CCTGGGGGTA ATCAGTGAGC TGAGGGTGGT CGGAGACCCT 
CGTGTTGCTG AGAGGTACTG CGAAGAGGAC GGCGATGACT CGGATATGGC TTCAGGTGAA 
GGAAGTGGGT ATGAGGAGAG CAGACCCCCC AAACCAGAGG AGAAACACAG ATGGACGACC 
ACCCCTCCAC CTTCAAGACC CATTCAGCAG CCACCGTTGT CCACTAAAGA GCAAGTGCTG 
AGAGAAACAG GAGCCCTTGG AGCCAAAGGA GAAAAGGGTG ACCGAGGGGA GAGAGGAGAG 
AAAGGAGGCA GAGGTCCTAG TGGACCTAAG GGCGATTCTG GCTTTGGTTC CAGCTCCAGG 
GGCGGGGCCC GTGGAGAAAA GGGAGAAGCA GGAGCAAAGG GAGAAAAGGG CAGTGCAGGC 
TTTGGCAACC AGGGGACAAA GGGAGAGCCA GGCTCTCCTG GGCCACCTGG CCCCCCAGGA 
CCTGTAGGGG ATTCTGTGTC CTGCAAAGAT GGATCTGCTG GAAACACCTG TCTCCAGGGA 
CCACCGGGCG CTGATGGAGA GCCAGGTGTT CCTGGTGAGG ACGGAAAGCC TGGTCCTCAA 
GGAGCTTCAG GATTTCCAGG TACCCCAGGT GACCAAGGAC CTAAAGGAGA GAAGGGGGAC 
ACTGGAGAGG GACCCCCTGG CCCCAGAGGA CCCCCAGGAC CTCCAGGACC CCCAGGAACA 
GGATTGAGAT CCACCTTTGT GGATATGGAA GGTTCAGGAT ACCCAGATTT GGAATCTGTG 
CGGGGACCAC CTGGTCCACC TGGCATCCCG GGCCCACCAG GTCCTCCTGG CACACCTGGT 
GCCTCAACAG CAGCCACAGG TTTGGGTTCT GGAGCAGCTG GCCCCCCAGG AAAAGATGGT 
GCCCCTGGTC AGCCTGGTTC GCCCGGGTTA CCAGGTACTG ATGGCTCACC GGGAGCGACG 
GGTCCCAAAG GAGAAAAGGG GAACACAGGT GAACTGGGTC TTCCAGGAGC AGTCGGACAG 
AAGGGAGACC AAGGAAGTCC TGGATTACCA GGATCTCCAG GGGAGGCTGG ACTGGCTGGT 
TTGCCTGGAC CTCGGGGACC CATGGGGCTA CCTGGACCAC CCGGACCAGG CTATCGCGTG 
GGTTTTGACG ATATGGAGGG ATCAGGTGGA GGCTTCATCA ACAGGCAACC TGGAGTCAGA 
GGACCAGAGG GAAAACAGGG TCCTCCGGGC TTCCCCGGAC TTCCAGGGAA ACCTGGCTTC 
CCTGGTCTAC CAGGGCCAAA AGGTAATGAT GGTCCTGAGG GGAAAGATGG ACGGCCAGGG 
TTGGATGGCT TCCCTGGACC TCAGGGACCA AAGGGTGACA GAGGCGACAA AGGAGACAGG 
GGTGAAGCGG GGCAGGATGG CACTGGACTA CCGGGCCCAC CTGGGCCACC TGGCCTTCCA 
GGAGAAATCA TCTACCGGTC ATCAGGCAAC ACTGACGGTG CTGTCGGCAG CGTGGGGCCT 
CAGGGAATAC CTGGACAACC TGGATTCCCC GGGCCAGCAG GGCCAAAAGG TGACAGGGGA 
GAACCTGGCT TTCCAGGCTT TGGAGAGAAG GGTGAGAAAG GTGAGCCAGG CTTAGCGATC 
GGACCCGATG GATATCGTCT GTCTCTGGAG GGACTAACAG GTCTGAAGGG AGAGAGGGGA 
ATACCTGGAC CAGTTGGACC TCCTGGCCCA TATGGCCCTC CTGGATTAAA GGGTGAAATT 
GGAATGCCAG GCAGATCTGG TCGTCCTGGT ATAAATGGAT ACAAGGGTGA GAAAGGAGAG 
CCTGGGACTG GTTCTGGATT TGCCTACCCA GGGCCACCAG GACCACCTGG ACCACCAGGC 
CCTCCAGGAC CAACCATCAA CCAAGATCGT TTCAATCGCT ACGATGACAC ACACAGAAAT 
TATCCAGCAG TCAAAGGGGA GAAAGGAGAG CATGGTGAAC AAGGCCTTCC AGGAATCCCT 
GGAACAGCCT CTAATTTTGA TATCTATGCA TACAAGAATG AACTGAAGGG AGAGCGTGGA 
GATCAAGGCC TGAAAGGAGA AAAGGGTGAG TCTGGGGGAT ATTATGACCC ACGTTTTGGT 
GGAGTACAAG GACCTCCTGG CACACCAGGC CTGCCTGGCC CCAAAGGGGA CTCAATAACT 
GGCCCTCCTG GACCCCAGGG GCCACCAGGA CAGCCAGGCA TTGGTTACGA TGGACCTGCA 
GGTTCTCCAG GACCACCCGG ACCACCTGGA GCTCCTGGAA CTAGCACATA CAATTACCCT 
GTCAGTCTGC CTGGGCCACC TGGTCCTCCC GGTCCACCTG GAATTCCAGG TCACTCCTCT 
GGGGTTACAG TTTTAAGGTC ATATGACACT ATGATTGCGA CTGCCAGACG TCAGCCAGAA 
GGAAACCTAA TCTACATCAT CGACAAAGCA GACCTGTACT TGAGAGTACG CAATGGACTA 
CGGCAAGTCA TGCTTGGAAA CTACAGTCCC TTCTTTAGAG AACTGGAGAA CGAGGTGGCA 
GAGGCCCAGC CTCCACCTGT CATCCTGTAT CCCCATTCCC AAGACCAGTC CCACAACAAC 
GGCGCTGGCC ATTACTCCCA GGGTGGACCT GCTATACAAC CCATTGATCC TCCACCACAA 
CCGCCTGTAA ACAACAACTA CCCTCCCCAC TATGAGCCCA GAGTCCCTGA AACGAGACAC 
ACGGGCCACA CAGAAGGACG ATCAGGCACT CAGACAGAGA CCCAACACCC CGTGACCCCA 
CAGCGACCAC CCAACCCGCC CGTACTGCAG CCTCTCGGGA ATGTTGAAAC ATCAGCATCA 
GCACTACATC TCATCGCCTT AAATGCCCCA CACACGGGTA ACATGCGAGG AATCCGCGGG 
GCAGATTTCC TCTGCTTCCA ACAGGCCCGA GCCATCGGAC TCAAGGGCAC CTTCAGAGCA 
TTCCTCTCCT CCAAGCTTCA AGACCTCTAC ACGATCGTCC GAAGGTCAGA CAGGGACAAC 
TTCCCCATCG TGAACCTCAA GGACCAAGTG TTGTTCAGAA GCTGGGAATC GATGTTTGGT 
GACGAAGCTA GCAAAATTAC GGAGAATGTA CCCATCTACT CCTTCGATGG CAGAGATATT 
CTCAGGGATA GTGCATGGCC AGAGAAAATG GTCTGGCATG GATCGAGCAA CAAAGGCCAC 
CGGCAAACAG ACCAGTACTG TGAAACATGG CGAGCGGGAG ATCGCGCTGT GACTGGTCTG 
GCATCATCAC TTAAGAGCGG CTACCTCCTG CAGCAGTCCG CCAGCAGCTG TTCTGGCTCC 
TACGTTGTTC TCTGCATAGA GAACGCCTTC ACGTCACACT CCAAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610514.1
CDS	173..4123
Translation	MKMRCARLQRLLCCVLVVIGLAASQWPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALTFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPAVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612212.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XVIII, alpha 1 (col18a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612212.1

ORF Insert Sequence:

ATGAAGATGA GGTGTGCACG GCTGCAGCGG CTCCTGTGCT GCGTCCTAGT GGTGATAGGT 
CTCGCTGCCT CCCAGTGGCC AGAGGACAAC GGCGTCTCCC TGCTGCAGCT GATCGGGGAT 
CCTCCTCCTC CTGAGATCAC CCAAGTCGAT GGGCCCGAAA ACAGCCCCGC TTACGTCTTT 
GGTCCTGACG CCAACACGGG ACAGTTGGCC CGTGCGCACC TCCCCAGTCC GTTCTACAGA 
GACTTTGCCC TCACCTTCCA CCTGAAGTCC AGCTCTAATA AGGGCGGAGT CGTCTTCTCC 
ATCACAGACG CCTCGCAGCA GATCATGTAC GTCGGTGTTA AACTCTCAGA TGTGCAGGGA 
CGCAACCAGA ACATCATCCT GTTTTACACG GAACCGGGCT CGGAGGAGTC GTACGAGGCG 
GCCAGCTTCC CCGTGCCGTC CACGGTGGAT ACCTGGACTC GCTTTGCCAT CGCCGTGAGG 
GACGACAAGG TAATGTTCTA CTTCAACTGT GAAGTGGACC CTCTGGTGAT GCCCATCGAG 
AGGTCCCCGG ATGAGATGGA GCTGGAGGCC GGAGCTGGGG TCTTCATCGG CCAAGCTGGA 
GGAGCCGATC CTGGAAAGTT CCTGGGGGTA ATCAGTGAGC TGAGGGTGGT CGGAGACCCT 
CGTGTTGCTG AGAGGTACTG CGAAGAGGAC GGCGATGACT CGGATATGGC TTCAGGTGAA 
GGAAGTGGGT ATGAGGAGAG CAGACCCCCC AAACCAGAGG AGAAACACAG ATGGACGACC 
ACCCCTCCAC CTTCAAGACC CATTCAGCAG CCACCGTTGT CCACTAAAGA GCAAGTGCTG 
AGAGAAACAG GAGCCCTTGG AGCCAAAGGA GAAAAGGGTG ACCGAGGGGA GAGAGGAGAG 
AAAGGAGGCA GAGGTCCTAG TGGACCTAAG GGCGATTCTG GCTTTGGTTC CAGCTCCAGG 
GGCGGGGCCC GTGGAGAAAA GGGAGAAGCA GGAGCAAAGG GAGAAAAGGG CAGTGCAGGC 
TTTGGCAACC AGGGGACAAA GGGAGAGCCA GGCTCTCCTG GGCCACCTGG CCCCCCAGGA 
CCTGTAGGGG ATTCTGTGTC CTGCAAAGAT GGATCTGCTG GAAACACCTG TCTCCAGGGA 
CCACCGGGCG CTGATGGAGA GCCAGGTGTT CCTGGTGAGG ACGGAAAGCC TGGTCCTCAA 
GGAGCTTCAG GATTTCCAGG TACCCCAGGT GACCAAGGAC CTAAAGGAGA GAAGGGGGAC 
ACTGGAGAGG GACCCCCTGG CCCCAGAGGA CCCCCAGGAC CTCCAGGACC CCCAGGAACA 
GGATTGAGAT CCACCTTTGT GGATATGGAA GGTTCAGGAT ACCCAGATTT GGAATCTGTG 
CGGGGACCAC CTGGTCCACC TGGCATCCCG GGCCCACCAG GTCCTCCTGG CACACCTGGT 
GCCTCAACAG CAGCCACAGG TTTGGGTTCT GGAGCAGCTG GCCCCCCAGG AAAAGATGGT 
GCCCCTGGTC AGCCTGGTTC GCCCGGGTTA CCAGGTACTG ATGGCTCACC GGGAGCGACG 
GGTCCCAAAG GAGAAAAGGG GAACACAGGT GAACTGGGTC TTCCAGGAGC AGTCGGACAG 
AAGGGAGACC AAGGAAGTCC TGGATTACCA GGATCTCCAG GGGAGGCTGG ACTGGCTGGT 
TTGCCTGGAC CTCGGGGACC CATGGGGCTA CCTGGACCAC CCGGACCAGG CTATCGCGTG 
GGTTTTGACG ATATGGAGGG ATCAGGTGGA GGCTTCATCA ACAGGCAACC TGGAGTCAGA 
GGACCAGAGG GAAAACAGGG TCCTCCGGGC TTCCCCGGAC TTCCAGGGAA ACCTGGCTTC 
CCTGGTCTAC CAGGGCCAAA AGGTAATGAT GGTCCTGAGG GGAAAGATGG ACGGCCAGGG 
TTGGATGGCT TCCCTGGACC TCAGGGACCA AAGGGTGACA GAGGCGACAA AGGAGACAGG 
GGTGAAGCGG GGCAGGATGG CACTGGACTA CCGGGCCCAC CTGGGCCACC TGGCCTTCCA 
GGAGAAATCA TCTACCGGTC ATCAGGCAAC ACTGACGGTG CTGTCGGCAG CGTGGGGCCT 
CAGGGAATAC CTGGACAACC TGGATTCCCC GGGCCAGCAG GGCCAAAAGG TGACAGGGGA 
GAACCTGGCT TTCCAGGCTT TGGAGAGAAG GGTGAGAAAG GTGAGCCAGG CTTAGCGATC 
GGACCCGATG GATATCGTCT GTCTCTGGAG GGACTAACAG GTCTGAAGGG AGAGAGGGGA 
ATACCTGGAC CAGTTGGACC TCCTGGCCCA TATGGCCCTC CTGGATTAAA GGGTGAAATT 
GGAATGCCAG GCAGATCTGG TCGTCCTGGT ATAAATGGAT ACAAGGGTGA GAAAGGAGAG 
CCTGGGACTG GTTCTGGATT TGCCTACCCA GGGCCACCAG GACCACCTGG ACCACCAGGC 
CCTCCAGGAC CAACCATCAA CCAAGATCGT TTCAATCGCT ACGATGACAC ACACAGAAAT 
TATCCAGCAG TCAAAGGGGA GAAAGGAGAG CATGGTGAAC AAGGCCTTCC AGGAATCCCT 
GGAACAGCCT CTAATTTTGA TATCTATGCA TACAAGAATG AACTGAAGGG AGAGCGTGGA 
GATCAAGGCC TGAAAGGAGA AAAGGGTGAG TCTGGGGGAT ATTATGACCC ACGTTTTGGT 
GGAGTACAAG GACCTCCTGG CACACCAGGC CTGCCTGGCC CCAAAGGGGA CTCAATAACT 
GGCCCTCCTG GACCCCAGGG GCCACCAGGA CAGCCAGGCA TTGGTTACGA TGGACCTGCA 
GGTTCTCCAG GACCACCCGG ACCACCTGGA GCTCCTGGAA CTAGCACATA CAATTACCCT 
GTCAGTCTGC CTGGGCCACC TGGTCCTCCC GGTCCACCTG GAATTCCAGG TCACTCCTCT 
GGGGTTACAG TTTTAAGGTC ATATGACACT ATGATTGCGA CTGCCAGACG TCAGCCAGAA 
GGAAACCTAA TCTACATCAT CGACAAAGCA GACCTGTACT TGAGAGTACG CAATGGACTA 
CGGCAAGTCA TGCTTGGAAA CTACAGTCCC TTCTTTAGAG AACTGGAGAA CGAGGTGGCA 
GAGGCCCAGC CTCCACCTGT CATCCTGTAT CCCCATTCCC AAGACCAGTC CCACAACAAC 
GGCGCTGGCC ATTACTCCCA GGGTGGACCT GCTATACAAC CCATTGATCC TCCACCACAA 
CCGCCTGTAA ACAACAACTA CCCTCCCCAC TATGAGCCCA GAGTCCCTGA AACGAGACAC 
ACGGGCCACA CAGAAGGACG ATCAGGCACT CAGACAGAGA CCCAACACCC CGTGACCCCA 
CAGCGACCAC CCAACCCGCC CGTACTGCAG CCTCTCGGGA ATGTTGAAAC ATCAGCATCA 
GCACTACATC TCATCGCCTT AAATGCCCCA CACACGGGTA ACATGCGAGG AATCCGCGGG 
GCAGATTTCC TCTGCTTCCA ACAGGCCCGA GCCATCGGAC TCAAGGGCAC CTTCAGAGCA 
TTCCTCTCCT CCAAGCTTCA AGACCTCTAC ACGATCGTCC GAAGGTCAGA CAGGGACAAC 
TTCCCCATCG TGAACCTCAA GGACCAAGTG TTGTTCAGAA GCTGGGAATC GATGTTTGGT 
GACGAAGCTA GCAAAATTAC GGAGAATGTA CCCATCTACT CCTTCGATGG CAGAGATATT 
CTCAGGGATA GTGCATGGCC AGAGAAAATG GTCTGGCATG GATCGAGCAA CAAAGGCCAC 
CGGCAAACAG ACCAGTACTG TGAAACATGG CGAGCGGGAG ATCGCGCTGT GACTGGTCTG 
GCATCATCAC TTAAGAGCGG CTACCTCCTG CAGCAGTCCG CCAGCAGCTG TTCTGGCTCC 
TACGTTGTTC TCTGCATAGA GAACGCCTTC ACGTCACACT CCAAAAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_011612198.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610500.2 Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo41076
Clone ID Related Accession (Same CDS sequence)	XM_011612198.2
Accession Version	XM_011612198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XVIII) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGCAGTCAAA GGGGAGAAAG GAGAGCATGG TGAACAAGGC 
CTTCCAGGAA TCCCTGGAAC AGCCTCTAAT TTTGATATCT ATGCATACAA GAATGAACTG 
AAGGGAGAGC GTGGAGATCA AGGCCTGAAA GGAGAAAAGG GTGAGTCTGG GGGATATTAT 
GACCCACGTT TTGGTGGAGT ACAAGGACCT CCTGGCACAC CAGGCCTGCC TGGCCCCAAA 
GGGGACTCAA TAACTGGCCC TCCTGGACCC CAGGGGCCAC CAGGACAGCC AGGCATTGGT 
TACGATGGAC CTGCAGGTTC TCCAGGACCA CCCGGACCAC CTGGAGCTCC TGGAACTAGC 
ACATACAATT ACCCTGTCAG TCTGCCTGGG CCACCTGGTC CTCCCGGTCC ACCTGGAATT 
CCAGGTCACT CCTCTGGGGT TACAGTTTTA AGGTCATATG ACACTATGAT TGCGACTGCC 
AGACGTCAGC CAGAAGGAAA TCTAATCTAC ATCATCGACA AAGCAGACCT GTACTTGAGA 
GTACGCAATG GACTACGGCA AGTCATGCTT GGAAACTACA GTCCCTTCTT TAGAGAACTG 
GAGAACGAGG TGGCAGAGGC CCAGCCTCCA CCTGTCATCC TGTATCCCCA TTCCCAAGAC 
CAGTCCCACA ACAATGGCGC TGGCCATTAC TCCCAGGGTG GACCTGCTAT ACAACCCATT 
GATCCTCCAC CACAACCGCC TGTAAACAAC AACAACTACC CTCCCCACTA TGAGCCCAGA 
GTCCCTGAAA CGAGACACAC GGGCCACACA GAAGGACGAT CAGGCACTCA GACAGAGACC 
CAACACCCCG TGACCCCACA GCGACCACCC AACCCGCCCG TACTGCAGCC TCTCGGGAAT 
GTTGAAACAT CAGCATCAGC ACTACATCTC ATCGCCTTAA ATGCCCCACA CACGGGTAAC 
ATGCGAGGAA TCCGCGGGGC AGATTTCCTC TGCTTCCAAC AGGCCCGAGC CATCGGACTC 
AAGGGCACCT TCAGAGCATT CCTCTCCTCC AAGCTTCAAG ACCTCTACAC GATCGTCCGA 
AGGTCAGACA GGGACAACTT CCCCATCGTG AACCTCAAGG ACCAAGTGTT GTTCAGAAGC 
TGGGAATCGA TGTTTGGTGA CGAAGCTAGC AAAATTACGG AGAATGTACC CATCTACTCC 
TTCGATGGCA GAGATATTCT CAGGGATAGT GCATGGCCAG AGAAAATGGT CTGGCATGGA 
TCGAGCAACA AAGGCCACCG GCAAACAGAC CAGTACTGTG AAACATGGCG AGCGGGAGAT 
CGCGCTGTGA CTGGTCTGGC ATCATCACTT AAGAGCGGCT ACCTCCTGCA GCAGTCCGCC 
AGCAGCTGTT CTGGCTCCTA CGTTGTTCTC TGCATAGAGA ACGCCTTCAC GTCACACTCC 
AAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610500.2
CDS	130..4338
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALIFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPAVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612198.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612198.2

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGCAGTCAAA GGGGAGAAAG GAGAGCATGG TGAACAAGGC 
CTTCCAGGAA TCCCTGGAAC AGCCTCTAAT TTTGATATCT ATGCATACAA GAATGAACTG 
AAGGGAGAGC GTGGAGATCA AGGCCTGAAA GGAGAAAAGG GTGAGTCTGG GGGATATTAT 
GACCCACGTT TTGGTGGAGT ACAAGGACCT CCTGGCACAC CAGGCCTGCC TGGCCCCAAA 
GGGGACTCAA TAACTGGCCC TCCTGGACCC CAGGGGCCAC CAGGACAGCC AGGCATTGGT 
TACGATGGAC CTGCAGGTTC TCCAGGACCA CCCGGACCAC CTGGAGCTCC TGGAACTAGC 
ACATACAATT ACCCTGTCAG TCTGCCTGGG CCACCTGGTC CTCCCGGTCC ACCTGGAATT 
CCAGGTCACT CCTCTGGGGT TACAGTTTTA AGGTCATATG ACACTATGAT TGCGACTGCC 
AGACGTCAGC CAGAAGGAAA TCTAATCTAC ATCATCGACA AAGCAGACCT GTACTTGAGA 
GTACGCAATG GACTACGGCA AGTCATGCTT GGAAACTACA GTCCCTTCTT TAGAGAACTG 
GAGAACGAGG TGGCAGAGGC CCAGCCTCCA CCTGTCATCC TGTATCCCCA TTCCCAAGAC 
CAGTCCCACA ACAATGGCGC TGGCCATTAC TCCCAGGGTG GACCTGCTAT ACAACCCATT 
GATCCTCCAC CACAACCGCC TGTAAACAAC AACAACTACC CTCCCCACTA TGAGCCCAGA 
GTCCCTGAAA CGAGACACAC GGGCCACACA GAAGGACGAT CAGGCACTCA GACAGAGACC 
CAACACCCCG TGACCCCACA GCGACCACCC AACCCGCCCG TACTGCAGCC TCTCGGGAAT 
GTTGAAACAT CAGCATCAGC ACTACATCTC ATCGCCTTAA ATGCCCCACA CACGGGTAAC 
ATGCGAGGAA TCCGCGGGGC AGATTTCCTC TGCTTCCAAC AGGCCCGAGC CATCGGACTC 
AAGGGCACCT TCAGAGCATT CCTCTCCTCC AAGCTTCAAG ACCTCTACAC GATCGTCCGA 
AGGTCAGACA GGGACAACTT CCCCATCGTG AACCTCAAGG ACCAAGTGTT GTTCAGAAGC 
TGGGAATCGA TGTTTGGTGA CGAAGCTAGC AAAATTACGG AGAATGTACC CATCTACTCC 
TTCGATGGCA GAGATATTCT CAGGGATAGT GCATGGCCAG AGAAAATGGT CTGGCATGGA 
TCGAGCAACA AAGGCCACCG GCAAACAGAC CAGTACTGTG AAACATGGCG AGCGGGAGAT 
CGCGCTGTGA CTGGTCTGGC ATCATCACTT AAGAGCGGCT ACCTCCTGCA GCAGTCCGCC 
AGCAGCTGTT CTGGCTCCTA CGTTGTTCTC TGCATAGAGA ACGCCTTCAC GTCACACTCC 
AAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_011612203.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610505.2 Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X2, mRNA.

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CloneID	OTo41077
Clone ID Related Accession (Same CDS sequence)	XM_011612203.2
Accession Version	XM_011612203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XVIII) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGTCAAAGGG GAGAAAGGAG AGCATGGTGA ACAAGGCCTT 
CCAGGAATCC CTGGAACAGC CTCTAATTTT GATATCTATG CATACAAGAA TGAACTGAAG 
GGAGAGCGTG GAGATCAAGG CCTGAAAGGA GAAAAGGGTG AGTCTGGGGG ATATTATGAC 
CCACGTTTTG GTGGAGTACA AGGACCTCCT GGCACACCAG GCCTGCCTGG CCCCAAAGGG 
GACTCAATAA CTGGCCCTCC TGGACCCCAG GGGCCACCAG GACAGCCAGG CATTGGTTAC 
GATGGACCTG CAGGTTCTCC AGGACCACCC GGACCACCTG GAGCTCCTGG AACTAGCACA 
TACAATTACC CTGTCAGTCT GCCTGGGCCA CCTGGTCCTC CCGGTCCACC TGGAATTCCA 
GGTCACTCCT CTGGGGTTAC AGTTTTAAGG TCATATGACA CTATGATTGC GACTGCCAGA 
CGTCAGCCAG AAGGAAATCT AATCTACATC ATCGACAAAG CAGACCTGTA CTTGAGAGTA 
CGCAATGGAC TACGGCAAGT CATGCTTGGA AACTACAGTC CCTTCTTTAG AGAACTGGAG 
AACGAGGTGG CAGAGGCCCA GCCTCCACCT GTCATCCTGT ATCCCCATTC CCAAGACCAG 
TCCCACAACA ATGGCGCTGG CCATTACTCC CAGGGTGGAC CTGCTATACA ACCCATTGAT 
CCTCCACCAC AACCGCCTGT AAACAACAAC AACTACCCTC CCCACTATGA GCCCAGAGTC 
CCTGAAACGA GACACACGGG CCACACAGAA GGACGATCAG GCACTCAGAC AGAGACCCAA 
CACCCCGTGA CCCCACAGCG ACCACCCAAC CCGCCCGTAC TGCAGCCTCT CGGGAATGTT 
GAAACATCAG CATCAGCACT ACATCTCATC GCCTTAAATG CCCCACACAC GGGTAACATG 
CGAGGAATCC GCGGGGCAGA TTTCCTCTGC TTCCAACAGG CCCGAGCCAT CGGACTCAAG 
GGCACCTTCA GAGCATTCCT CTCCTCCAAG CTTCAAGACC TCTACACGAT CGTCCGAAGG 
TCAGACAGGG ACAACTTCCC CATCGTGAAC CTCAAGGACC AAGTGTTGTT CAGAAGCTGG 
GAATCGATGT TTGGTGACGA AGCTAGCAAA ATTACGGAGA ATGTACCCAT CTACTCCTTC 
GATGGCAGAG ATATTCTCAG GGATAGTGCA TGGCCAGAGA AAATGGTCTG GCATGGATCG 
AGCAACAAAG GCCACCGGCA AACAGACCAG TACTGTGAAA CATGGCGAGC GGGAGATCGC 
GCTGTGACTG GTCTGGCATC ATCACTTAAG AGCGGCTACC TCCTGCAGCA GTCCGCCAGC 
AGCTGTTCTG GCTCCTACGT TGTTCTCTGC ATAGAGAACG CCTTCACGTC ACACTCCAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610505.2
CDS	130..4335
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALIFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612203.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612203.2

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGTCAAAGGG GAGAAAGGAG AGCATGGTGA ACAAGGCCTT 
CCAGGAATCC CTGGAACAGC CTCTAATTTT GATATCTATG CATACAAGAA TGAACTGAAG 
GGAGAGCGTG GAGATCAAGG CCTGAAAGGA GAAAAGGGTG AGTCTGGGGG ATATTATGAC 
CCACGTTTTG GTGGAGTACA AGGACCTCCT GGCACACCAG GCCTGCCTGG CCCCAAAGGG 
GACTCAATAA CTGGCCCTCC TGGACCCCAG GGGCCACCAG GACAGCCAGG CATTGGTTAC 
GATGGACCTG CAGGTTCTCC AGGACCACCC GGACCACCTG GAGCTCCTGG AACTAGCACA 
TACAATTACC CTGTCAGTCT GCCTGGGCCA CCTGGTCCTC CCGGTCCACC TGGAATTCCA 
GGTCACTCCT CTGGGGTTAC AGTTTTAAGG TCATATGACA CTATGATTGC GACTGCCAGA 
CGTCAGCCAG AAGGAAATCT AATCTACATC ATCGACAAAG CAGACCTGTA CTTGAGAGTA 
CGCAATGGAC TACGGCAAGT CATGCTTGGA AACTACAGTC CCTTCTTTAG AGAACTGGAG 
AACGAGGTGG CAGAGGCCCA GCCTCCACCT GTCATCCTGT ATCCCCATTC CCAAGACCAG 
TCCCACAACA ATGGCGCTGG CCATTACTCC CAGGGTGGAC CTGCTATACA ACCCATTGAT 
CCTCCACCAC AACCGCCTGT AAACAACAAC AACTACCCTC CCCACTATGA GCCCAGAGTC 
CCTGAAACGA GACACACGGG CCACACAGAA GGACGATCAG GCACTCAGAC AGAGACCCAA 
CACCCCGTGA CCCCACAGCG ACCACCCAAC CCGCCCGTAC TGCAGCCTCT CGGGAATGTT 
GAAACATCAG CATCAGCACT ACATCTCATC GCCTTAAATG CCCCACACAC GGGTAACATG 
CGAGGAATCC GCGGGGCAGA TTTCCTCTGC TTCCAACAGG CCCGAGCCAT CGGACTCAAG 
GGCACCTTCA GAGCATTCCT CTCCTCCAAG CTTCAAGACC TCTACACGAT CGTCCGAAGG 
TCAGACAGGG ACAACTTCCC CATCGTGAAC CTCAAGGACC AAGTGTTGTT CAGAAGCTGG 
GAATCGATGT TTGGTGACGA AGCTAGCAAA ATTACGGAGA ATGTACCCAT CTACTCCTTC 
GATGGCAGAG ATATTCTCAG GGATAGTGCA TGGCCAGAGA AAATGGTCTG GCATGGATCG 
AGCAACAAAG GCCACCGGCA AACAGACCAG TACTGTGAAA CATGGCGAGC GGGAGATCGC 
GCTGTGACTG GTCTGGCATC ATCACTTAAG AGCGGCTACC TCCTGCAGCA GTCCGCCAGC 
AGCTGTTCTG GCTCCTACGT TGTTCTCTGC ATAGAGAACG CCTTCACGTC ACACTCCAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_029833608.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029689468.1 Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X3, mRNA.

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CloneID	OTo41078
Clone ID Related Accession (Same CDS sequence)	XM_029833608.1
Accession Version	XM_029833608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XVIII) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGCAGTCAAA GGGGAGAAAG GAGAGCATGG TGAACAAGGC 
CTTCCAGGAA TCCCTGCCTC TAATTTTGAT ATCTATGCAT ACAAGAATGA ACTGAAGGGA 
GAGCGTGGAG ATCAAGGCCT GAAAGGAGAA AAGGGTGAGT CTGGGGGATA TTATGACCCA 
CGTTTTGGTG GAGTACAAGG ACCTCCTGGC ACACCAGGCC TGCCTGGCCC CAAAGGGGAC 
TCAATAACTG GCCCTCCTGG ACCCCAGGGG CCACCAGGAC AGCCAGGCAT TGGTTACGAT 
GGACCTGCAG GTTCTCCAGG ACCACCCGGA CCACCTGGAG CTCCTGGAAC TAGCACATAC 
AATTACCCTG TCAGTCTGCC TGGGCCACCT GGTCCTCCCG GTCCACCTGG AATTCCAGGT 
CACTCCTCTG GGGTTACAGT TTTAAGGTCA TATGACACTA TGATTGCGAC TGCCAGACGT 
CAGCCAGAAG GAAATCTAAT CTACATCATC GACAAAGCAG ACCTGTACTT GAGAGTACGC 
AATGGACTAC GGCAAGTCAT GCTTGGAAAC TACAGTCCCT TCTTTAGAGA ACTGGAGAAC 
GAGGTGGCAG AGGCCCAGCC TCCACCTGTC ATCCTGTATC CCCATTCCCA AGACCAGTCC 
CACAACAATG GCGCTGGCCA TTACTCCCAG GGTGGACCTG CTATACAACC CATTGATCCT 
CCACCACAAC CGCCTGTAAA CAACAACAAC TACCCTCCCC ACTATGAGCC CAGAGTCCCT 
GAAACGAGAC ACACGGGCCA CACAGAAGGA CGATCAGGCA CTCAGACAGA GACCCAACAC 
CCCGTGACCC CACAGCGACC ACCCAACCCG CCCGTACTGC AGCCTCTCGG GAATGTTGAA 
ACATCAGCAT CAGCACTACA TCTCATCGCC TTAAATGCCC CACACACGGG TAACATGCGA 
GGAATCCGCG GGGCAGATTT CCTCTGCTTC CAACAGGCCC GAGCCATCGG ACTCAAGGGC 
ACCTTCAGAG CATTCCTCTC CTCCAAGCTT CAAGACCTCT ACACGATCGT CCGAAGGTCA 
GACAGGGACA ACTTCCCCAT CGTGAACCTC AAGGACCAAG TGTTGTTCAG AAGCTGGGAA 
TCGATGTTTG GTGACGAAGC TAGCAAAATT ACGGAGAATG TACCCATCTA CTCCTTCGAT 
GGCAGAGATA TTCTCAGGGA TAGTGCATGG CCAGAGAAAA TGGTCTGGCA TGGATCGAGC 
AACAAAGGCC ACCGGCAAAC AGACCAGTAC TGTGAAACAT GGCGAGCGGG AGATCGCGCT 
GTGACTGGTC TGGCATCATC ACTTAAGAGC GGCTACCTCC TGCAGCAGTC CGCCAGCAGC 
TGTTCTGGCT CCTACGTTGT TCTCTGCATA GAGAACGCCT TCACGTCACA CTCCAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029689468.1
CDS	130..4332
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALIFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPAVKGEKGEHGEQGLPGIPASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_029833608.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029833608.1

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGCAGTCAAA GGGGAGAAAG GAGAGCATGG TGAACAAGGC 
CTTCCAGGAA TCCCTGCCTC TAATTTTGAT ATCTATGCAT ACAAGAATGA ACTGAAGGGA 
GAGCGTGGAG ATCAAGGCCT GAAAGGAGAA AAGGGTGAGT CTGGGGGATA TTATGACCCA 
CGTTTTGGTG GAGTACAAGG ACCTCCTGGC ACACCAGGCC TGCCTGGCCC CAAAGGGGAC 
TCAATAACTG GCCCTCCTGG ACCCCAGGGG CCACCAGGAC AGCCAGGCAT TGGTTACGAT 
GGACCTGCAG GTTCTCCAGG ACCACCCGGA CCACCTGGAG CTCCTGGAAC TAGCACATAC 
AATTACCCTG TCAGTCTGCC TGGGCCACCT GGTCCTCCCG GTCCACCTGG AATTCCAGGT 
CACTCCTCTG GGGTTACAGT TTTAAGGTCA TATGACACTA TGATTGCGAC TGCCAGACGT 
CAGCCAGAAG GAAATCTAAT CTACATCATC GACAAAGCAG ACCTGTACTT GAGAGTACGC 
AATGGACTAC GGCAAGTCAT GCTTGGAAAC TACAGTCCCT TCTTTAGAGA ACTGGAGAAC 
GAGGTGGCAG AGGCCCAGCC TCCACCTGTC ATCCTGTATC CCCATTCCCA AGACCAGTCC 
CACAACAATG GCGCTGGCCA TTACTCCCAG GGTGGACCTG CTATACAACC CATTGATCCT 
CCACCACAAC CGCCTGTAAA CAACAACAAC TACCCTCCCC ACTATGAGCC CAGAGTCCCT 
GAAACGAGAC ACACGGGCCA CACAGAAGGA CGATCAGGCA CTCAGACAGA GACCCAACAC 
CCCGTGACCC CACAGCGACC ACCCAACCCG CCCGTACTGC AGCCTCTCGG GAATGTTGAA 
ACATCAGCAT CAGCACTACA TCTCATCGCC TTAAATGCCC CACACACGGG TAACATGCGA 
GGAATCCGCG GGGCAGATTT CCTCTGCTTC CAACAGGCCC GAGCCATCGG ACTCAAGGGC 
ACCTTCAGAG CATTCCTCTC CTCCAAGCTT CAAGACCTCT ACACGATCGT CCGAAGGTCA 
GACAGGGACA ACTTCCCCAT CGTGAACCTC AAGGACCAAG TGTTGTTCAG AAGCTGGGAA 
TCGATGTTTG GTGACGAAGC TAGCAAAATT ACGGAGAATG TACCCATCTA CTCCTTCGAT 
GGCAGAGATA TTCTCAGGGA TAGTGCATGG CCAGAGAAAA TGGTCTGGCA TGGATCGAGC 
AACAAAGGCC ACCGGCAAAC AGACCAGTAC TGTGAAACAT GGCGAGCGGG AGATCGCGCT 
GTGACTGGTC TGGCATCATC ACTTAAGAGC GGCTACCTCC TGCAGCAGTC CGCCAGCAGC 
TGTTCTGGCT CCTACGTTGT TCTCTGCATA GAGAACGCCT TCACGTCACA CTCCAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_011612206.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610508.2 Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo41079
Clone ID Related Accession (Same CDS sequence)	XM_011612206.2
Accession Version	XM_011612206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XVIII) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGACGATAT GGAGGGATCA GGTGGAGGCT TCATCAACAG GCAACCTGGA 
GTCAGAGGAC CAGAGGGAAA ACAGGGTCCT CCGGGCTTCC CCGGACTTCC AGGGAAACCT 
GGCTTCCCTG GTCTACCAGG GCCAAAAGGT AATGATGGTC CTGAGGGGAA AGATGGACGG 
CCAGGGTTGG ATGGCTTCCC TGGACCTCAG GGACCAAAGG GTGACAGAGG CGACAAAGGA 
GACAGGGGTG AAGCGGGGCA GGATGGCACT GGACTACCGG GCCCACCTGG GCCACCTGGC 
CTTCCAGGAG AAATCATCTA CCGGTCATCA GGCAACACTG ACGGTGCTGT CGGCAGCGTG 
GGGCCTCAGG GAATACCTGG ACAACCTGGA TTCCCCGGGC CAGCAGGGCC AAAAGGTGAC 
AGGGGAGAAC CTGGCTTTCC AGGCTTTGGA GAGAAGGGTG AGAAAGGTGA GCCAGGCTTA 
GCGATCGGAC CCGATGGATA TCGTCTGTCT CTGGAGGGAC TAACAGGTCT GAAGGGAGAG 
AGGGGAATAC CTGGACCAGT TGGACCTCCT GGCCCATATG GCCCTCCTGG ATTAAAGGGT 
GAAATTGGAA TGCCAGGCAG ATCTGGTCGT CCTGGTATAA ATGGATACAA GGGTGAGAAA 
GGAGAGCCTG GGACTGGTTC TGGATTTGCC TACCCAGGGC CACCAGGACC ACCTGGACCA 
CCAGGCCCTC CAGGACCAAC CATCAACCAA GATCGTTTCA ATCGCTACGA TGACACACAC 
AGAAATTATC CAGCAGTCAA AGGGGAGAAA GGAGAGCATG GTGAACAAGG CCTTCCAGGA 
ATCCCTGGAA CAGCCTCTAA TTTTGATATC TATGCATACA AGAATGAACT GAAGGGAGAG 
CGTGGAGATC AAGGCCTGAA AGGAGAAAAG GGTGAGTCTG GGGGATATTA TGACCCACGT 
TTTGGTGGAG TACAAGGACC TCCTGGCACA CCAGGCCTGC CTGGCCCCAA AGGGGACTCA 
ATAACTGGCC CTCCTGGACC CCAGGGGCCA CCAGGACAGC CAGGCATTGG TTACGATGGA 
CCTGCAGGTT CTCCAGGACC ACCCGGACCA CCTGGAGCTC CTGGAACTAG CACATACAAT 
TACCCTGTCA GTCTGCCTGG GCCACCTGGT CCTCCCGGTC CACCTGGAAT TCCAGGTCAC 
TCCTCTGGGG TTACAGTTTT AAGGTCATAT GACACTATGA TTGCGACTGC CAGACGTCAG 
CCAGAAGGAA ATCTAATCTA CATCATCGAC AAAGCAGACC TGTACTTGAG AGTACGCAAT 
GGACTACGGC AAGTCATGCT TGGAAACTAC AGTCCCTTCT TTAGAGAACT GGAGAACGAG 
GTGGCAGAGG CCCAGCCTCC ACCTGTCATC CTGTATCCCC ATTCCCAAGA CCAGTCCCAC 
AACAATGGCG CTGGCCATTA CTCCCAGGGT GGACCTGCTA TACAACCCAT TGATCCTCCA 
CCACAACCGC CTGTAAACAA CAACAACTAC CCTCCCCACT ATGAGCCCAG AGTCCCTGAA 
ACGAGACACA CGGGCCACAC AGAAGGACGA TCAGGCACTC AGACAGAGAC CCAACACCCC 
GTGACCCCAC AGCGACCACC CAACCCGCCC GTACTGCAGC CTCTCGGGAA TGTTGAAACA 
TCAGCATCAG CACTACATCT CATCGCCTTA AATGCCCCAC ACACGGGTAA CATGCGAGGA 
ATCCGCGGGG CAGATTTCCT CTGCTTCCAA CAGGCCCGAG CCATCGGACT CAAGGGCACC 
TTCAGAGCAT TCCTCTCCTC CAAGCTTCAA GACCTCTACA CGATCGTCCG AAGGTCAGAC 
AGGGACAACT TCCCCATCGT GAACCTCAAG GACCAAGTGT TGTTCAGAAG CTGGGAATCG 
ATGTTTGGTG ACGAAGCTAG CAAAATTACG GAGAATGTAC CCATCTACTC CTTCGATGGC 
AGAGATATTC TCAGGGATAG TGCATGGCCA GAGAAAATGG TCTGGCATGG ATCGAGCAAC 
AAAGGCCACC GGCAAACAGA CCAGTACTGT GAAACATGGC GAGCGGGAGA TCGCGCTGTG 
ACTGGTCTGG CATCATCACT TAAGAGCGGC TACCTCCTGC AGCAGTCCGC CAGCAGCTGT 
TCTGGCTCCT ACGTTGTTCT CTGCATAGAG AACGCCTTCA CGTCACACTC CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610508.2
CDS	130..4329
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALIFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPAVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612206.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612206.2

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGACGATAT GGAGGGATCA GGTGGAGGCT TCATCAACAG GCAACCTGGA 
GTCAGAGGAC CAGAGGGAAA ACAGGGTCCT CCGGGCTTCC CCGGACTTCC AGGGAAACCT 
GGCTTCCCTG GTCTACCAGG GCCAAAAGGT AATGATGGTC CTGAGGGGAA AGATGGACGG 
CCAGGGTTGG ATGGCTTCCC TGGACCTCAG GGACCAAAGG GTGACAGAGG CGACAAAGGA 
GACAGGGGTG AAGCGGGGCA GGATGGCACT GGACTACCGG GCCCACCTGG GCCACCTGGC 
CTTCCAGGAG AAATCATCTA CCGGTCATCA GGCAACACTG ACGGTGCTGT CGGCAGCGTG 
GGGCCTCAGG GAATACCTGG ACAACCTGGA TTCCCCGGGC CAGCAGGGCC AAAAGGTGAC 
AGGGGAGAAC CTGGCTTTCC AGGCTTTGGA GAGAAGGGTG AGAAAGGTGA GCCAGGCTTA 
GCGATCGGAC CCGATGGATA TCGTCTGTCT CTGGAGGGAC TAACAGGTCT GAAGGGAGAG 
AGGGGAATAC CTGGACCAGT TGGACCTCCT GGCCCATATG GCCCTCCTGG ATTAAAGGGT 
GAAATTGGAA TGCCAGGCAG ATCTGGTCGT CCTGGTATAA ATGGATACAA GGGTGAGAAA 
GGAGAGCCTG GGACTGGTTC TGGATTTGCC TACCCAGGGC CACCAGGACC ACCTGGACCA 
CCAGGCCCTC CAGGACCAAC CATCAACCAA GATCGTTTCA ATCGCTACGA TGACACACAC 
AGAAATTATC CAGCAGTCAA AGGGGAGAAA GGAGAGCATG GTGAACAAGG CCTTCCAGGA 
ATCCCTGGAA CAGCCTCTAA TTTTGATATC TATGCATACA AGAATGAACT GAAGGGAGAG 
CGTGGAGATC AAGGCCTGAA AGGAGAAAAG GGTGAGTCTG GGGGATATTA TGACCCACGT 
TTTGGTGGAG TACAAGGACC TCCTGGCACA CCAGGCCTGC CTGGCCCCAA AGGGGACTCA 
ATAACTGGCC CTCCTGGACC CCAGGGGCCA CCAGGACAGC CAGGCATTGG TTACGATGGA 
CCTGCAGGTT CTCCAGGACC ACCCGGACCA CCTGGAGCTC CTGGAACTAG CACATACAAT 
TACCCTGTCA GTCTGCCTGG GCCACCTGGT CCTCCCGGTC CACCTGGAAT TCCAGGTCAC 
TCCTCTGGGG TTACAGTTTT AAGGTCATAT GACACTATGA TTGCGACTGC CAGACGTCAG 
CCAGAAGGAA ATCTAATCTA CATCATCGAC AAAGCAGACC TGTACTTGAG AGTACGCAAT 
GGACTACGGC AAGTCATGCT TGGAAACTAC AGTCCCTTCT TTAGAGAACT GGAGAACGAG 
GTGGCAGAGG CCCAGCCTCC ACCTGTCATC CTGTATCCCC ATTCCCAAGA CCAGTCCCAC 
AACAATGGCG CTGGCCATTA CTCCCAGGGT GGACCTGCTA TACAACCCAT TGATCCTCCA 
CCACAACCGC CTGTAAACAA CAACAACTAC CCTCCCCACT ATGAGCCCAG AGTCCCTGAA 
ACGAGACACA CGGGCCACAC AGAAGGACGA TCAGGCACTC AGACAGAGAC CCAACACCCC 
GTGACCCCAC AGCGACCACC CAACCCGCCC GTACTGCAGC CTCTCGGGAA TGTTGAAACA 
TCAGCATCAG CACTACATCT CATCGCCTTA AATGCCCCAC ACACGGGTAA CATGCGAGGA 
ATCCGCGGGG CAGATTTCCT CTGCTTCCAA CAGGCCCGAG CCATCGGACT CAAGGGCACC 
TTCAGAGCAT TCCTCTCCTC CAAGCTTCAA GACCTCTACA CGATCGTCCG AAGGTCAGAC 
AGGGACAACT TCCCCATCGT GAACCTCAAG GACCAAGTGT TGTTCAGAAG CTGGGAATCG 
ATGTTTGGTG ACGAAGCTAG CAAAATTACG GAGAATGTAC CCATCTACTC CTTCGATGGC 
AGAGATATTC TCAGGGATAG TGCATGGCCA GAGAAAATGG TCTGGCATGG ATCGAGCAAC 
AAAGGCCACC GGCAAACAGA CCAGTACTGT GAAACATGGC GAGCGGGAGA TCGCGCTGTG 
ACTGGTCTGG CATCATCACT TAAGAGCGGC TACCTCCTGC AGCAGTCCGC CAGCAGCTGT 
TCTGGCTCCT ACGTTGTTCT CTGCATAGAG AACGCCTTCA CGTCACACTC CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_029833618.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029689478.1 Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTo41080
Clone ID Related Accession (Same CDS sequence)	XM_029833618.1
Accession Version	XM_029833618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XVIII) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGTCAAAGGG GAGAAAGGAG AGCATGGTGA ACAAGGCCTT 
CCAGGAATCC CTGCCTCTAA TTTTGATATC TATGCATACA AGAATGAACT GAAGGGAGAG 
CGTGGAGATC AAGGCCTGAA AGGAGAAAAG GGTGAGTCTG GGGGATATTA TGACCCACGT 
TTTGGTGGAG TACAAGGACC TCCTGGCACA CCAGGCCTGC CTGGCCCCAA AGGGGACTCA 
ATAACTGGCC CTCCTGGACC CCAGGGGCCA CCAGGACAGC CAGGCATTGG TTACGATGGA 
CCTGCAGGTT CTCCAGGACC ACCCGGACCA CCTGGAGCTC CTGGAACTAG CACATACAAT 
TACCCTGTCA GTCTGCCTGG GCCACCTGGT CCTCCCGGTC CACCTGGAAT TCCAGGTCAC 
TCCTCTGGGG TTACAGTTTT AAGGTCATAT GACACTATGA TTGCGACTGC CAGACGTCAG 
CCAGAAGGAA ATCTAATCTA CATCATCGAC AAAGCAGACC TGTACTTGAG AGTACGCAAT 
GGACTACGGC AAGTCATGCT TGGAAACTAC AGTCCCTTCT TTAGAGAACT GGAGAACGAG 
GTGGCAGAGG CCCAGCCTCC ACCTGTCATC CTGTATCCCC ATTCCCAAGA CCAGTCCCAC 
AACAATGGCG CTGGCCATTA CTCCCAGGGT GGACCTGCTA TACAACCCAT TGATCCTCCA 
CCACAACCGC CTGTAAACAA CAACAACTAC CCTCCCCACT ATGAGCCCAG AGTCCCTGAA 
ACGAGACACA CGGGCCACAC AGAAGGACGA TCAGGCACTC AGACAGAGAC CCAACACCCC 
GTGACCCCAC AGCGACCACC CAACCCGCCC GTACTGCAGC CTCTCGGGAA TGTTGAAACA 
TCAGCATCAG CACTACATCT CATCGCCTTA AATGCCCCAC ACACGGGTAA CATGCGAGGA 
ATCCGCGGGG CAGATTTCCT CTGCTTCCAA CAGGCCCGAG CCATCGGACT CAAGGGCACC 
TTCAGAGCAT TCCTCTCCTC CAAGCTTCAA GACCTCTACA CGATCGTCCG AAGGTCAGAC 
AGGGACAACT TCCCCATCGT GAACCTCAAG GACCAAGTGT TGTTCAGAAG CTGGGAATCG 
ATGTTTGGTG ACGAAGCTAG CAAAATTACG GAGAATGTAC CCATCTACTC CTTCGATGGC 
AGAGATATTC TCAGGGATAG TGCATGGCCA GAGAAAATGG TCTGGCATGG ATCGAGCAAC 
AAAGGCCACC GGCAAACAGA CCAGTACTGT GAAACATGGC GAGCGGGAGA TCGCGCTGTG 
ACTGGTCTGG CATCATCACT TAAGAGCGGC TACCTCCTGC AGCAGTCCGC CAGCAGCTGT 
TCTGGCTCCT ACGTTGTTCT CTGCATAGAG AACGCCTTCA CGTCACACTC CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029689478.1
CDS	130..4329
Translation	MKIQSQVYILVALGVVSTDAWFWSRTGPTTLAPTPAVESEGSGSPADSGDPPTESSAKLRAEIIDEGHDFYNVVQTWNETTVAPVLTTPMQLQNENGSEPEPAGISSPISKPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALIFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPVKGEKGEHGEQGLPGIPASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_029833618.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029833618.1

ORF Insert Sequence:

ATGAAGATCC AGAGTCAAGT TTATATTTTG GTGGCTTTGG GGGTGGTCTC CACTGATGCT 
TGGTTTTGGT CCCGGACTGG TCCCACAACT TTGGCCCCAA CGCCGGCAGT GGAGTCCGAA 
GGATCTGGCA GTCCAGCAGA TAGCGGAGAT CCACCAACGG AGAGCTCTGC AAAGCTCAGA 
GCAGAGATAA TTGATGAGGG ACATGACTTC TACAACGTCG TGCAGACTTG GAATGAGACC 
ACAGTGGCTC CTGTGCTGAC CACTCCAATG CAGCTGCAGA ATGAAAATGG CTCAGAACCG 
GAACCTGCGG GGATTTCATC ACCCATTAGT AAACCTGAGG ACAACGGCGT CTCCCTGCTG 
CAGCTGATCG GGGATCCTCC TCCTCCTGAG ATCACCCAAG TCGATGGGCC CGAAAACAGC 
CCCGCTTACG TCTTTGGTCC TGACGCCAAC ACGGGACAGT TGGCCCGTGC GCACCTCCCC 
AGTCCGTTCT ACAGAGACTT TGCCCTCATC TTCCACCTGA AGTCCAGCTC TAATAAGGGC 
GGAGTCGTCT TCTCCATCAC AGACGCCTCG CAGCAGATCA TGTACGTCGG TGTTAAACTC 
TCAGATGTGC AGGGACGCAA CCAGAACATC ATCCTGTTTT ACACGGAACC GGGCTCGGAG 
GAGTCGTACG AGGCGGCCAG CTTCCCCGTG CCGTCCACGG TGGATACCTG GACTCGCTTT 
GCCATCGCCG TGAGGGACGA CAAGGTAATG TTCTACTTCA ACTGTGAAGT GGACCCTCTG 
GTGATGCCCA TCGAGAGGTC CCCGGATGAG ATGGAGCTGG AGGCCGGAGC TGGGGTCTTC 
ATCGGCCAAG CTGGAGGAGC CGATCCTGGA AAGTTCCTGG GGGTAATCAG TGAGCTGAGG 
GTGGTCGGAG ACCCTCGTGT TGCTGAGAGG TACTGCGAAG AGGACGGCGA TGACTCGGAT 
ATGGCTTCAG GTGAAGGAAG TGGGTATGAG GAGAGCAGAC CCCCCAAACC AGAGGAGAAA 
CACAGATGGA CGACCACCCC TCCACCTTCA AGACCCATTC AGCAGCCACC GTTGTCCACT 
AAAGAGCAAG TGCTGAGAGA AACAGGAGCC CTTGGAGCCA AAGGAGAAAA GGGTGACCGA 
GGGGAGAGAG GAGAGAAAGG AGGCAGAGGT CCTAGTGGAC CTAAGGGCGA TTCTGGCTTT 
GGTTCCAGCT CCAGGGGCGG GGCCCGTGGA GAAAAGGGAG AAGCAGGAGC AAAGGGAGAA 
AAGGGCAGTG CAGGCTTTGG CAACCAGGGG ACAAAGGGAG AGCCAGGCTC TCCTGGGCCA 
CCTGGCCCCC CAGGACCTGT AGGGGATTCT GTGTCCTGCA AAGATGGATC TGCTGGAAAC 
ACCTGTCTCC AGGGACCACC GGGCGCTGAT GGAGAGCCAG GTGTTCCTGG TGAGGACGGA 
AAGCCTGGTC CTCAAGGAGC TTCAGGATTT CCAGGTACCC CAGGTGACCA AGGACCTAAA 
GGAGAGAAGG GGGACACTGG AGAGGGACCC CCTGGCCCCA GAGGACCCCC AGGACCTCCA 
GGACCCCCAG GAACAGGATT GAGATCCACC TTTGTGGATA TGGAAGGTTC AGGATACCCA 
GATTTGGAAT CTGTGCGGGG ACCACCTGGT CCACCTGGCA TCCCGGGCCC ACCAGGTCCT 
CCTGGCACAC CTGGTGCCTC AACAGCAGCC ACAGGTTTGG GTTCTGGAGC AGCTGGCCCC 
CCAGGAAAAG ATGGTGCCCC TGGTCAGCCT GGTTCACCCG GGTTACCAGG TACTGATGGC 
TCACCGGGAG CGACGGGTCC CAAAGGAGAA AAGGGGAACA CAGGTGAACT GGGTCTTCCA 
GGAGCAGTCG GACAGAAGGG AGACCAAGGA AGTCCTGGAT TACCAGGATC TCCAGGGGAG 
GCTGGACTGG CTGGTTTGCC TGGACCTCGG GGACCCATGG GGCTACCTGG ACCACCCGGA 
CCAGGCTATC GCGTGGGTTT TGACGATATG GAGGGATCAG GTGGAGGCTT CATCAACAGG 
CAACCTGGAG TCAGAGGACC AGAGGGAAAA CAGGGTCCTC CGGGCTTCCC CGGACTTCCA 
GGGAAACCTG GCTTCCCTGG TCTACCAGGG CCAAAAGGTA ATGATGGTCC TGAGGGGAAA 
GATGGACGGC CAGGGTTGGA TGGCTTCCCT GGACCTCAGG GACCAAAGGG TGACAGAGGC 
GACAAAGGAG ACAGGGGTGA AGCGGGGCAG GATGGCACTG GACTACCGGG CCCACCTGGG 
CCACCTGGCC TTCCAGGAGA AATCATCTAC CGGTCATCAG GCAACACTGA CGGTGCTGTC 
GGCAGCGTGG GGCCTCAGGG AATACCTGGA CAACCTGGAT TCCCCGGGCC AGCAGGGCCA 
AAAGGTGACA GGGGAGAACC TGGCTTTCCA GGCTTTGGAG AGAAGGGTGA GAAAGGTGAG 
CCAGGCTTAG CGATCGGACC CGATGGATAT CGTCTGTCTC TGGAGGGACT AACAGGTCTG 
AAGGGAGAGA GGGGAATACC TGGACCAGTT GGACCTCCTG GCCCATATGG CCCTCCTGGA 
TTAAAGGGTG AAATTGGAAT GCCAGGCAGA TCTGGTCGTC CTGGTATAAA TGGATACAAG 
GGTGAGAAAG GAGAGCCTGG GACTGGTTCT GGATTTGCCT ACCCAGGGCC ACCAGGACCA 
CCTGGACCAC CAGGCCCTCC AGGACCAACC ATCAACCAAG ATCGTTTCAA TCGCTACGAT 
GACACACACA GAAATTATCC AGTCAAAGGG GAGAAAGGAG AGCATGGTGA ACAAGGCCTT 
CCAGGAATCC CTGCCTCTAA TTTTGATATC TATGCATACA AGAATGAACT GAAGGGAGAG 
CGTGGAGATC AAGGCCTGAA AGGAGAAAAG GGTGAGTCTG GGGGATATTA TGACCCACGT 
TTTGGTGGAG TACAAGGACC TCCTGGCACA CCAGGCCTGC CTGGCCCCAA AGGGGACTCA 
ATAACTGGCC CTCCTGGACC CCAGGGGCCA CCAGGACAGC CAGGCATTGG TTACGATGGA 
CCTGCAGGTT CTCCAGGACC ACCCGGACCA CCTGGAGCTC CTGGAACTAG CACATACAAT 
TACCCTGTCA GTCTGCCTGG GCCACCTGGT CCTCCCGGTC CACCTGGAAT TCCAGGTCAC 
TCCTCTGGGG TTACAGTTTT AAGGTCATAT GACACTATGA TTGCGACTGC CAGACGTCAG 
CCAGAAGGAA ATCTAATCTA CATCATCGAC AAAGCAGACC TGTACTTGAG AGTACGCAAT 
GGACTACGGC AAGTCATGCT TGGAAACTAC AGTCCCTTCT TTAGAGAACT GGAGAACGAG 
GTGGCAGAGG CCCAGCCTCC ACCTGTCATC CTGTATCCCC ATTCCCAAGA CCAGTCCCAC 
AACAATGGCG CTGGCCATTA CTCCCAGGGT GGACCTGCTA TACAACCCAT TGATCCTCCA 
CCACAACCGC CTGTAAACAA CAACAACTAC CCTCCCCACT ATGAGCCCAG AGTCCCTGAA 
ACGAGACACA CGGGCCACAC AGAAGGACGA TCAGGCACTC AGACAGAGAC CCAACACCCC 
GTGACCCCAC AGCGACCACC CAACCCGCCC GTACTGCAGC CTCTCGGGAA TGTTGAAACA 
TCAGCATCAG CACTACATCT CATCGCCTTA AATGCCCCAC ACACGGGTAA CATGCGAGGA 
ATCCGCGGGG CAGATTTCCT CTGCTTCCAA CAGGCCCGAG CCATCGGACT CAAGGGCACC 
TTCAGAGCAT TCCTCTCCTC CAAGCTTCAA GACCTCTACA CGATCGTCCG AAGGTCAGAC 
AGGGACAACT TCCCCATCGT GAACCTCAAG GACCAAGTGT TGTTCAGAAG CTGGGAATCG 
ATGTTTGGTG ACGAAGCTAG CAAAATTACG GAGAATGTAC CCATCTACTC CTTCGATGGC 
AGAGATATTC TCAGGGATAG TGCATGGCCA GAGAAAATGG TCTGGCATGG ATCGAGCAAC 
AAAGGCCACC GGCAAACAGA CCAGTACTGT GAAACATGGC GAGCGGGAGA TCGCGCTGTG 
ACTGGTCTGG CATCATCACT TAAGAGCGGC TACCTCCTGC AGCAGTCCGC CAGCAGCTGT 
TCTGGCTCCT ACGTTGTTCT CTGCATAGAG AACGCCTTCA CGTCACACTC CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col18a1 ( XM_011612212.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011610514.2 Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X6, mRNA.

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CloneID	OTo41081
Clone ID Related Accession (Same CDS sequence)	XM_011612212.2
Accession Version	XM_011612212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XVIII) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011612212.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATGA GGTGTGCACG GCTGCAGCGG CTCCTGTGCT GCGTCCTAGT GGTGATAGGT 
CTCGCTGCCT CCCAGTGGCC AGAGGACAAC GGCGTCTCCC TGCTGCAGCT GATCGGGGAT 
CCTCCTCCTC CTGAGATCAC CCAAGTCGAT GGGCCCGAAA ACAGCCCCGC TTACGTCTTT 
GGTCCTGACG CCAACACGGG ACAGTTGGCC CGTGCGCACC TCCCCAGTCC GTTCTACAGA 
GACTTTGCCC TCATCTTCCA CCTGAAGTCC AGCTCTAATA AGGGCGGAGT CGTCTTCTCC 
ATCACAGACG CCTCGCAGCA GATCATGTAC GTCGGTGTTA AACTCTCAGA TGTGCAGGGA 
CGCAACCAGA ACATCATCCT GTTTTACACG GAACCGGGCT CGGAGGAGTC GTACGAGGCG 
GCCAGCTTCC CCGTGCCGTC CACGGTGGAT ACCTGGACTC GCTTTGCCAT CGCCGTGAGG 
GACGACAAGG TAATGTTCTA CTTCAACTGT GAAGTGGACC CTCTGGTGAT GCCCATCGAG 
AGGTCCCCGG ATGAGATGGA GCTGGAGGCC GGAGCTGGGG TCTTCATCGG CCAAGCTGGA 
GGAGCCGATC CTGGAAAGTT CCTGGGGGTA ATCAGTGAGC TGAGGGTGGT CGGAGACCCT 
CGTGTTGCTG AGAGGTACTG CGAAGAGGAC GGCGATGACT CGGATATGGC TTCAGGTGAA 
GGAAGTGGGT ATGAGGAGAG CAGACCCCCC AAACCAGAGG AGAAACACAG ATGGACGACC 
ACCCCTCCAC CTTCAAGACC CATTCAGCAG CCACCGTTGT CCACTAAAGA GCAAGTGCTG 
AGAGAAACAG GAGCCCTTGG AGCCAAAGGA GAAAAGGGTG ACCGAGGGGA GAGAGGAGAG 
AAAGGAGGCA GAGGTCCTAG TGGACCTAAG GGCGATTCTG GCTTTGGTTC CAGCTCCAGG 
GGCGGGGCCC GTGGAGAAAA GGGAGAAGCA GGAGCAAAGG GAGAAAAGGG CAGTGCAGGC 
TTTGGCAACC AGGGGACAAA GGGAGAGCCA GGCTCTCCTG GGCCACCTGG CCCCCCAGGA 
CCTGTAGGGG ATTCTGTGTC CTGCAAAGAT GGATCTGCTG GAAACACCTG TCTCCAGGGA 
CCACCGGGCG CTGATGGAGA GCCAGGTGTT CCTGGTGAGG ACGGAAAGCC TGGTCCTCAA 
GGAGCTTCAG GATTTCCAGG TACCCCAGGT GACCAAGGAC CTAAAGGAGA GAAGGGGGAC 
ACTGGAGAGG GACCCCCTGG CCCCAGAGGA CCCCCAGGAC CTCCAGGACC CCCAGGAACA 
GGATTGAGAT CCACCTTTGT GGATATGGAA GGTTCAGGAT ACCCAGATTT GGAATCTGTG 
CGGGGACCAC CTGGTCCACC TGGCATCCCG GGCCCACCAG GTCCTCCTGG CACACCTGGT 
GCCTCAACAG CAGCCACAGG TTTGGGTTCT GGAGCAGCTG GCCCCCCAGG AAAAGATGGT 
GCCCCTGGTC AGCCTGGTTC ACCCGGGTTA CCAGGTACTG ATGGCTCACC GGGAGCGACG 
GGTCCCAAAG GAGAAAAGGG GAACACAGGT GAACTGGGTC TTCCAGGAGC AGTCGGACAG 
AAGGGAGACC AAGGAAGTCC TGGATTACCA GGATCTCCAG GGGAGGCTGG ACTGGCTGGT 
TTGCCTGGAC CTCGGGGACC CATGGGGCTA CCTGGACCAC CCGGACCAGG CTATCGCGTG 
GGTTTTGACG ATATGGAGGG ATCAGGTGGA GGCTTCATCA ACAGGCAACC TGGAGTCAGA 
GGACCAGAGG GAAAACAGGG TCCTCCGGGC TTCCCCGGAC TTCCAGGGAA ACCTGGCTTC 
CCTGGTCTAC CAGGGCCAAA AGGTAATGAT GGTCCTGAGG GGAAAGATGG ACGGCCAGGG 
TTGGATGGCT TCCCTGGACC TCAGGGACCA AAGGGTGACA GAGGCGACAA AGGAGACAGG 
GGTGAAGCGG GGCAGGATGG CACTGGACTA CCGGGCCCAC CTGGGCCACC TGGCCTTCCA 
GGAGAAATCA TCTACCGGTC ATCAGGCAAC ACTGACGGTG CTGTCGGCAG CGTGGGGCCT 
CAGGGAATAC CTGGACAACC TGGATTCCCC GGGCCAGCAG GGCCAAAAGG TGACAGGGGA 
GAACCTGGCT TTCCAGGCTT TGGAGAGAAG GGTGAGAAAG GTGAGCCAGG CTTAGCGATC 
GGACCCGATG GATATCGTCT GTCTCTGGAG GGACTAACAG GTCTGAAGGG AGAGAGGGGA 
ATACCTGGAC CAGTTGGACC TCCTGGCCCA TATGGCCCTC CTGGATTAAA GGGTGAAATT 
GGAATGCCAG GCAGATCTGG TCGTCCTGGT ATAAATGGAT ACAAGGGTGA GAAAGGAGAG 
CCTGGGACTG GTTCTGGATT TGCCTACCCA GGGCCACCAG GACCACCTGG ACCACCAGGC 
CCTCCAGGAC CAACCATCAA CCAAGATCGT TTCAATCGCT ACGATGACAC ACACAGAAAT 
TATCCAGCAG TCAAAGGGGA GAAAGGAGAG CATGGTGAAC AAGGCCTTCC AGGAATCCCT 
GGAACAGCCT CTAATTTTGA TATCTATGCA TACAAGAATG AACTGAAGGG AGAGCGTGGA 
GATCAAGGCC TGAAAGGAGA AAAGGGTGAG TCTGGGGGAT ATTATGACCC ACGTTTTGGT 
GGAGTACAAG GACCTCCTGG CACACCAGGC CTGCCTGGCC CCAAAGGGGA CTCAATAACT 
GGCCCTCCTG GACCCCAGGG GCCACCAGGA CAGCCAGGCA TTGGTTACGA TGGACCTGCA 
GGTTCTCCAG GACCACCCGG ACCACCTGGA GCTCCTGGAA CTAGCACATA CAATTACCCT 
GTCAGTCTGC CTGGGCCACC TGGTCCTCCC GGTCCACCTG GAATTCCAGG TCACTCCTCT 
GGGGTTACAG TTTTAAGGTC ATATGACACT ATGATTGCGA CTGCCAGACG TCAGCCAGAA 
GGAAATCTAA TCTACATCAT CGACAAAGCA GACCTGTACT TGAGAGTACG CAATGGACTA 
CGGCAAGTCA TGCTTGGAAA CTACAGTCCC TTCTTTAGAG AACTGGAGAA CGAGGTGGCA 
GAGGCCCAGC CTCCACCTGT CATCCTGTAT CCCCATTCCC AAGACCAGTC CCACAACAAT 
GGCGCTGGCC ATTACTCCCA GGGTGGACCT GCTATACAAC CCATTGATCC TCCACCACAA 
CCGCCTGTAA ACAACAACAA CTACCCTCCC CACTATGAGC CCAGAGTCCC TGAAACGAGA 
CACACGGGCC ACACAGAAGG ACGATCAGGC ACTCAGACAG AGACCCAACA CCCCGTGACC 
CCACAGCGAC CACCCAACCC GCCCGTACTG CAGCCTCTCG GGAATGTTGA AACATCAGCA 
TCAGCACTAC ATCTCATCGC CTTAAATGCC CCACACACGG GTAACATGCG AGGAATCCGC 
GGGGCAGATT TCCTCTGCTT CCAACAGGCC CGAGCCATCG GACTCAAGGG CACCTTCAGA 
GCATTCCTCT CCTCCAAGCT TCAAGACCTC TACACGATCG TCCGAAGGTC AGACAGGGAC 
AACTTCCCCA TCGTGAACCT CAAGGACCAA GTGTTGTTCA GAAGCTGGGA ATCGATGTTT 
GGTGACGAAG CTAGCAAAAT TACGGAGAAT GTACCCATCT ACTCCTTCGA TGGCAGAGAT 
ATTCTCAGGG ATAGTGCATG GCCAGAGAAA ATGGTCTGGC ATGGATCGAG CAACAAAGGC 
CACCGGCAAA CAGACCAGTA CTGTGAAACA TGGCGAGCGG GAGATCGCGC TGTGACTGGT 
CTGGCATCAT CACTTAAGAG CGGCTACCTC CTGCAGCAGT CCGCCAGCAG CTGTTCTGGC 
TCCTACGTTG TTCTCTGCAT AGAGAACGCC TTCACGTCAC ACTCCAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011610514.2
CDS	190..4143
Translation	MKMRCARLQRLLCCVLVVIGLAASQWPEDNGVSLLQLIGDPPPPEITQVDGPENSPAYVFGPDANTGQLARAHLPSPFYRDFALIFHLKSSSNKGGVVFSITDASQQIMYVGVKLSDVQGRNQNIILFYTEPGSEESYEAASFPVPSTVDTWTRFAIAVRDDKVMFYFNCEVDPLVMPIERSPDEMELEAGAGVFIGQAGGADPGKFLGVISELRVVGDPRVAERYCEEDGDDSDMASGEGSGYEESRPPKPEEKHRWTTTPPPSRPIQQPPLSTKEQVLRETGALGAKGEKGDRGERGEKGGRGPSGPKGDSGFGSSSRGGARGEKGEAGAKGEKGSAGFGNQGTKGEPGSPGPPGPPGPVGDSVSCKDGSAGNTCLQGPPGADGEPGVPGEDGKPGPQGASGFPGTPGDQGPKGEKGDTGEGPPGPRGPPGPPGPPGTGLRSTFVDMEGSGYPDLESVRGPPGPPGIPGPPGPPGTPGASTAATGLGSGAAGPPGKDGAPGQPGSPGLPGTDGSPGATGPKGEKGNTGELGLPGAVGQKGDQGSPGLPGSPGEAGLAGLPGPRGPMGLPGPPGPGYRVGFDDMEGSGGGFINRQPGVRGPEGKQGPPGFPGLPGKPGFPGLPGPKGNDGPEGKDGRPGLDGFPGPQGPKGDRGDKGDRGEAGQDGTGLPGPPGPPGLPGEIIYRSSGNTDGAVGSVGPQGIPGQPGFPGPAGPKGDRGEPGFPGFGEKGEKGEPGLAIGPDGYRLSLEGLTGLKGERGIPGPVGPPGPYGPPGLKGEIGMPGRSGRPGINGYKGEKGEPGTGSGFAYPGPPGPPGPPGPPGPTINQDRFNRYDDTHRNYPAVKGEKGEHGEQGLPGIPGTASNFDIYAYKNELKGERGDQGLKGEKGESGGYYDPRFGGVQGPPGTPGLPGPKGDSITGPPGPQGPPGQPGIGYDGPAGSPGPPGPPGAPGTSTYNYPVSLPGPPGPPGPPGIPGHSSGVTVLRSYDTMIATARRQPEGNLIYIIDKADLYLRVRNGLRQVMLGNYSPFFRELENEVAEAQPPPVILYPHSQDQSHNNGAGHYSQGGPAIQPIDPPPQPPVNNNNYPPHYEPRVPETRHTGHTEGRSGTQTETQHPVTPQRPPNPPVLQPLGNVETSASALHLIALNAPHTGNMRGIRGADFLCFQQARAIGLKGTFRAFLSSKLQDLYTIVRRSDRDNFPIVNLKDQVLFRSWESMFGDEASKITENVPIYSFDGRDILRDSAWPEKMVWHGSSNKGHRQTDQYCETWRAGDRAVTGLASSLKSGYLLQQSASSCSGSYVVLCIENAFTSHSKK

Target ORF information:

RefSeq Version	XM_011612212.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen type XVIII alpha 1 chain (col18a1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011612212.2

ORF Insert Sequence:

ATGAAGATGA GGTGTGCACG GCTGCAGCGG CTCCTGTGCT GCGTCCTAGT GGTGATAGGT 
CTCGCTGCCT CCCAGTGGCC AGAGGACAAC GGCGTCTCCC TGCTGCAGCT GATCGGGGAT 
CCTCCTCCTC CTGAGATCAC CCAAGTCGAT GGGCCCGAAA ACAGCCCCGC TTACGTCTTT 
GGTCCTGACG CCAACACGGG ACAGTTGGCC CGTGCGCACC TCCCCAGTCC GTTCTACAGA 
GACTTTGCCC TCATCTTCCA CCTGAAGTCC AGCTCTAATA AGGGCGGAGT CGTCTTCTCC 
ATCACAGACG CCTCGCAGCA GATCATGTAC GTCGGTGTTA AACTCTCAGA TGTGCAGGGA 
CGCAACCAGA ACATCATCCT GTTTTACACG GAACCGGGCT CGGAGGAGTC GTACGAGGCG 
GCCAGCTTCC CCGTGCCGTC CACGGTGGAT ACCTGGACTC GCTTTGCCAT CGCCGTGAGG 
GACGACAAGG TAATGTTCTA CTTCAACTGT GAAGTGGACC CTCTGGTGAT GCCCATCGAG 
AGGTCCCCGG ATGAGATGGA GCTGGAGGCC GGAGCTGGGG TCTTCATCGG CCAAGCTGGA 
GGAGCCGATC CTGGAAAGTT CCTGGGGGTA ATCAGTGAGC TGAGGGTGGT CGGAGACCCT 
CGTGTTGCTG AGAGGTACTG CGAAGAGGAC GGCGATGACT CGGATATGGC TTCAGGTGAA 
GGAAGTGGGT ATGAGGAGAG CAGACCCCCC AAACCAGAGG AGAAACACAG ATGGACGACC 
ACCCCTCCAC CTTCAAGACC CATTCAGCAG CCACCGTTGT CCACTAAAGA GCAAGTGCTG 
AGAGAAACAG GAGCCCTTGG AGCCAAAGGA GAAAAGGGTG ACCGAGGGGA GAGAGGAGAG 
AAAGGAGGCA GAGGTCCTAG TGGACCTAAG GGCGATTCTG GCTTTGGTTC CAGCTCCAGG 
GGCGGGGCCC GTGGAGAAAA GGGAGAAGCA GGAGCAAAGG GAGAAAAGGG CAGTGCAGGC 
TTTGGCAACC AGGGGACAAA GGGAGAGCCA GGCTCTCCTG GGCCACCTGG CCCCCCAGGA 
CCTGTAGGGG ATTCTGTGTC CTGCAAAGAT GGATCTGCTG GAAACACCTG TCTCCAGGGA 
CCACCGGGCG CTGATGGAGA GCCAGGTGTT CCTGGTGAGG ACGGAAAGCC TGGTCCTCAA 
GGAGCTTCAG GATTTCCAGG TACCCCAGGT GACCAAGGAC CTAAAGGAGA GAAGGGGGAC 
ACTGGAGAGG GACCCCCTGG CCCCAGAGGA CCCCCAGGAC CTCCAGGACC CCCAGGAACA 
GGATTGAGAT CCACCTTTGT GGATATGGAA GGTTCAGGAT ACCCAGATTT GGAATCTGTG 
CGGGGACCAC CTGGTCCACC TGGCATCCCG GGCCCACCAG GTCCTCCTGG CACACCTGGT 
GCCTCAACAG CAGCCACAGG TTTGGGTTCT GGAGCAGCTG GCCCCCCAGG AAAAGATGGT 
GCCCCTGGTC AGCCTGGTTC ACCCGGGTTA CCAGGTACTG ATGGCTCACC GGGAGCGACG 
GGTCCCAAAG GAGAAAAGGG GAACACAGGT GAACTGGGTC TTCCAGGAGC AGTCGGACAG 
AAGGGAGACC AAGGAAGTCC TGGATTACCA GGATCTCCAG GGGAGGCTGG ACTGGCTGGT 
TTGCCTGGAC CTCGGGGACC CATGGGGCTA CCTGGACCAC CCGGACCAGG CTATCGCGTG 
GGTTTTGACG ATATGGAGGG ATCAGGTGGA GGCTTCATCA ACAGGCAACC TGGAGTCAGA 
GGACCAGAGG GAAAACAGGG TCCTCCGGGC TTCCCCGGAC TTCCAGGGAA ACCTGGCTTC 
CCTGGTCTAC CAGGGCCAAA AGGTAATGAT GGTCCTGAGG GGAAAGATGG ACGGCCAGGG 
TTGGATGGCT TCCCTGGACC TCAGGGACCA AAGGGTGACA GAGGCGACAA AGGAGACAGG 
GGTGAAGCGG GGCAGGATGG CACTGGACTA CCGGGCCCAC CTGGGCCACC TGGCCTTCCA 
GGAGAAATCA TCTACCGGTC ATCAGGCAAC ACTGACGGTG CTGTCGGCAG CGTGGGGCCT 
CAGGGAATAC CTGGACAACC TGGATTCCCC GGGCCAGCAG GGCCAAAAGG TGACAGGGGA 
GAACCTGGCT TTCCAGGCTT TGGAGAGAAG GGTGAGAAAG GTGAGCCAGG CTTAGCGATC 
GGACCCGATG GATATCGTCT GTCTCTGGAG GGACTAACAG GTCTGAAGGG AGAGAGGGGA 
ATACCTGGAC CAGTTGGACC TCCTGGCCCA TATGGCCCTC CTGGATTAAA GGGTGAAATT 
GGAATGCCAG GCAGATCTGG TCGTCCTGGT ATAAATGGAT ACAAGGGTGA GAAAGGAGAG 
CCTGGGACTG GTTCTGGATT TGCCTACCCA GGGCCACCAG GACCACCTGG ACCACCAGGC 
CCTCCAGGAC CAACCATCAA CCAAGATCGT TTCAATCGCT ACGATGACAC ACACAGAAAT 
TATCCAGCAG TCAAAGGGGA GAAAGGAGAG CATGGTGAAC AAGGCCTTCC AGGAATCCCT 
GGAACAGCCT CTAATTTTGA TATCTATGCA TACAAGAATG AACTGAAGGG AGAGCGTGGA 
GATCAAGGCC TGAAAGGAGA AAAGGGTGAG TCTGGGGGAT ATTATGACCC ACGTTTTGGT 
GGAGTACAAG GACCTCCTGG CACACCAGGC CTGCCTGGCC CCAAAGGGGA CTCAATAACT 
GGCCCTCCTG GACCCCAGGG GCCACCAGGA CAGCCAGGCA TTGGTTACGA TGGACCTGCA 
GGTTCTCCAG GACCACCCGG ACCACCTGGA GCTCCTGGAA CTAGCACATA CAATTACCCT 
GTCAGTCTGC CTGGGCCACC TGGTCCTCCC GGTCCACCTG GAATTCCAGG TCACTCCTCT 
GGGGTTACAG TTTTAAGGTC ATATGACACT ATGATTGCGA CTGCCAGACG TCAGCCAGAA 
GGAAATCTAA TCTACATCAT CGACAAAGCA GACCTGTACT TGAGAGTACG CAATGGACTA 
CGGCAAGTCA TGCTTGGAAA CTACAGTCCC TTCTTTAGAG AACTGGAGAA CGAGGTGGCA 
GAGGCCCAGC CTCCACCTGT CATCCTGTAT CCCCATTCCC AAGACCAGTC CCACAACAAT 
GGCGCTGGCC ATTACTCCCA GGGTGGACCT GCTATACAAC CCATTGATCC TCCACCACAA 
CCGCCTGTAA ACAACAACAA CTACCCTCCC CACTATGAGC CCAGAGTCCC TGAAACGAGA 
CACACGGGCC ACACAGAAGG ACGATCAGGC ACTCAGACAG AGACCCAACA CCCCGTGACC 
CCACAGCGAC CACCCAACCC GCCCGTACTG CAGCCTCTCG GGAATGTTGA AACATCAGCA 
TCAGCACTAC ATCTCATCGC CTTAAATGCC CCACACACGG GTAACATGCG AGGAATCCGC 
GGGGCAGATT TCCTCTGCTT CCAACAGGCC CGAGCCATCG GACTCAAGGG CACCTTCAGA 
GCATTCCTCT CCTCCAAGCT TCAAGACCTC TACACGATCG TCCGAAGGTC AGACAGGGAC 
AACTTCCCCA TCGTGAACCT CAAGGACCAA GTGTTGTTCA GAAGCTGGGA ATCGATGTTT 
GGTGACGAAG CTAGCAAAAT TACGGAGAAT GTACCCATCT ACTCCTTCGA TGGCAGAGAT 
ATTCTCAGGG ATAGTGCATG GCCAGAGAAA ATGGTCTGGC ATGGATCGAG CAACAAAGGC 
CACCGGCAAA CAGACCAGTA CTGTGAAACA TGGCGAGCGG GAGATCGCGC TGTGACTGGT 
CTGGCATCAT CACTTAAGAG CGGCTACCTC CTGCAGCAGT CCGCCAGCAG CTGTTCTGGC 
TCCTACGTTG TTCTCTGCAT AGAGAACGCC TTCACGTCAC ACTCCAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	col18a1
Entrez Gene ID	101077615
Full Name	collagen type XVIII alpha 1 chain
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

col18a1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

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