ccar1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	ccar1
Entrez Gene ID	101076714
Full Name	cell division cycle and apoptosis regulator 1
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029835176.1	XP_029691036.1	cell division cycle and apoptosis regulator protein 1 isoform X2
XM_011603318.1	XP_011601620.1	cell division cycle and apoptosis regulator protein 1 isoform X1
XM_011603317.1	XP_011601619.1	cell division cycle and apoptosis regulator protein 1 isoform X1
XM_011603317.2	XP_011601619.1	cell division cycle and apoptosis regulator protein 1 isoform X1
XM_011603319.1	XP_011601621.1	cell division cycle and apoptosis regulator protein 1 isoform X2
XM_011603318.2	XP_011601620.1	cell division cycle and apoptosis regulator protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following ccar1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ccar1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo65233	XM_011603318.2 Latest version!	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo65233	XM_011603317.2 Latest version!	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo65234	XM_029835176.1 Latest version!	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo29840	XM_011603318.1 Latest version!	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo29840	XM_011603317.1 Latest version!	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OTo29841	XM_011603319.1 Latest version!	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ccar1 ( XM_011603317.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601619.1 Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo65233
Clone ID Related Accession (Same CDS sequence)	XM_011603317.2 , XM_011603318.2
Accession Version	XM_011603317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cell division cycle and apoptosis regulator protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011603317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCGCG TTCGAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC AGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGCGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601619.1
CDS	325..4053
Translation	MAQFGGQKNPPWATQFAATAVSQPGHSGQSLDLNSLHSLGVQQPSLLGASPSVYPQQSALAAVSLSNQSAANYQLSQQTAALQQQAAAAAAAALQQSQINTALQQYQQQQQQQQQPPPQQPPQQQLYNVPHQLPQPQQALLSQFIPDPQPPVPLPTSMSLSNPQQTAQITVSYPTPRSSHQQQTQPQKQRVFTGVVNKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEAVYNPNMPFKWNAQRIQTLPQLPNQSHQQQPQPLPQASPQLGGLYNEPGMQLRYSDLHSAPDNRQNNQPQPPNMMKTAPAMLQSLPPPNTFSVPSQPPPPLLQAQLSAASLAPLLHNPPQPLLPQPPPKDVFAGGLLQPPVRMMSQPQPVRRIEPPPRFPNRNDRGPELILRSKEERSRDRDRERRRSRERSPARKRSRDRSPRRDRSPRRPRRVVPRYTVQFSKFSLDSSSCDMMELRRRYQSLYIPSDFFDAVFTWVDAFPMTRPFQLNNACNFHILHKEVDPLVKNTAVLDPPDANHTYSAKVMLLANPSLEELYHKSCALAEDTQEVKDSFQHPARLIKFLVGMRGKDEAMAIGGHWSPSLDGADPERDPSVLIKTAIRCCKALTGIDLSLCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFLPDVWHCLPTRSEWEVLSRRLREQLAEKLSAERKEADGEQDEEEKDDGESKDVSTPTHWAKLDPKSMKVNDLRKELDCRSLSSKGLKSQLIARLTKQLKVEEQVEESKEPDKPESKIPEEEEPSRTEEEKEEEERKRQEELDRQRRERRYILPDEPTILVNPNWAAKNGKFDCSVMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGYRIYKALAAIPVKDEKKEKKNKKEAEKRDVKKEKEEENEEPQAKKAKEDEEDKKKSEEKTLKKEPSQDEEENEDEGSTANAEEYDPMEAEDAEDDDDDDDDKDDEDSNDRDRRDRRDDRKSSRESASKDKEKKQMVTHNRELLMAFVYFDQSHCGYLLERDLEEILYTLGLHLSRAQIKKLLNKPVIRESCYYRKLTDNGKDEPVQSMNEAQIENLTGNRSLLPAPKARVKAEPSESGNLIVYNGAMVDVGSMMQKLEKSEKTREEIEQKLMVQDAKLEEDAKVKAQLEKSNKALNRELEEVKRTLNQTEQTLTSVKEQKTVYHDHIVKTSNSLMSTVKGLLGLMSKNKDEHLDLEVALDQASQANGSDE

Target ORF information:

RefSeq Version	XM_011603317.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603317.2

ORF Insert Sequence:

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCGCG TTCGAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC AGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGCGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccar1 ( XM_011603318.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601620.1 Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo65233
Clone ID Related Accession (Same CDS sequence)	XM_011603317.2 , XM_011603318.2
Accession Version	XM_011603318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cell division cycle and apoptosis regulator protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011603318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCGCG TTCGAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC AGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGCGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601620.1
CDS	123..3851
Translation	MAQFGGQKNPPWATQFAATAVSQPGHSGQSLDLNSLHSLGVQQPSLLGASPSVYPQQSALAAVSLSNQSAANYQLSQQTAALQQQAAAAAAAALQQSQINTALQQYQQQQQQQQQPPPQQPPQQQLYNVPHQLPQPQQALLSQFIPDPQPPVPLPTSMSLSNPQQTAQITVSYPTPRSSHQQQTQPQKQRVFTGVVNKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEAVYNPNMPFKWNAQRIQTLPQLPNQSHQQQPQPLPQASPQLGGLYNEPGMQLRYSDLHSAPDNRQNNQPQPPNMMKTAPAMLQSLPPPNTFSVPSQPPPPLLQAQLSAASLAPLLHNPPQPLLPQPPPKDVFAGGLLQPPVRMMSQPQPVRRIEPPPRFPNRNDRGPELILRSKEERSRDRDRERRRSRERSPARKRSRDRSPRRDRSPRRPRRVVPRYTVQFSKFSLDSSSCDMMELRRRYQSLYIPSDFFDAVFTWVDAFPMTRPFQLNNACNFHILHKEVDPLVKNTAVLDPPDANHTYSAKVMLLANPSLEELYHKSCALAEDTQEVKDSFQHPARLIKFLVGMRGKDEAMAIGGHWSPSLDGADPERDPSVLIKTAIRCCKALTGIDLSLCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFLPDVWHCLPTRSEWEVLSRRLREQLAEKLSAERKEADGEQDEEEKDDGESKDVSTPTHWAKLDPKSMKVNDLRKELDCRSLSSKGLKSQLIARLTKQLKVEEQVEESKEPDKPESKIPEEEEPSRTEEEKEEEERKRQEELDRQRRERRYILPDEPTILVNPNWAAKNGKFDCSVMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGYRIYKALAAIPVKDEKKEKKNKKEAEKRDVKKEKEEENEEPQAKKAKEDEEDKKKSEEKTLKKEPSQDEEENEDEGSTANAEEYDPMEAEDAEDDDDDDDDKDDEDSNDRDRRDRRDDRKSSRESASKDKEKKQMVTHNRELLMAFVYFDQSHCGYLLERDLEEILYTLGLHLSRAQIKKLLNKPVIRESCYYRKLTDNGKDEPVQSMNEAQIENLTGNRSLLPAPKARVKAEPSESGNLIVYNGAMVDVGSMMQKLEKSEKTREEIEQKLMVQDAKLEEDAKVKAQLEKSNKALNRELEEVKRTLNQTEQTLTSVKEQKTVYHDHIVKTSNSLMSTVKGLLGLMSKNKDEHLDLEVALDQASQANGSDE

Target ORF information:

RefSeq Version	XM_011603318.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603318.2

ORF Insert Sequence:

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCGCG TTCGAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC AGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGCGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccar1 ( XM_029835176.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029691036.1 Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X3, mRNA.

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CloneID	OTo65234
Clone ID Related Accession (Same CDS sequence)	XM_029835176.1
Accession Version	XM_029835176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cell division cycle and apoptosis regulator protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042288.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGC CCCCCGTGCC GTTGCCCACC AGCATGAGCT TGTCCAACCC TCAGCAGACA 
GCCCAAATTA CCGTGTCCTA CCCGACGCCG CGTTCGAGTC ACCAACAACA GACGCAGCCC 
CAGAAGCAGC GAGTCTTTAC TGGCGTCGTC AATAAGCTAC ACGACACGTT TGGCTTTGTA 
GATGAAGATG TTTTCTTTCA GCTGAGTGCG GTGAAGGGGA AGACTCCTCA GGTGGGTGAC 
AGAGTGCTCG TGGAAGCTGT GTACAACCCA AACATGCCGT TCAAGTGGAA TGCCCAACGG 
ATCCAAACCT TACCTCAGCT TCCAAACCAG TCGCATCAGC AGCAGCCTCA GCCTTTACCT 
CAAGCTTCCC CTCAGCTCGG CGGCCTTTAC AATGAACCAG GGATGCAGCT GCGCTACTCA 
GATCTTCACT CTGCTCCAGA CAACCGACAA AATAACCAGC CACAACCTCC TAATATGATG 
AAGACGGCCC CGGCCATGCT ACAATCCCTA CCTCCCCCAA ATACGTTCAG TGTCCCATCC 
CAGCCCCCTC CTCCACTTCT CCAAGCCCAG CTGTCTGCTG CCTCTTTGGC CCCTCTTCTG 
CACAACCCTC CCCAGCCTCT GCTCCCACAG CCTCCCCCCA AAGATGTCTT TGCCGGTGGT 
CTGCTTCAGC CCCCTGTCAG AATGATGTCA CAGCCCCAGC CTGTCCGGCG CATTGAACCC 
CCACCGCGTT TTCCCAATCG CAATGACCGG GGGCCTGAAC TCATCCTGAG AAGTAAAGAG 
GAACGCAGTC GGGACAGAGA CCGCGAGCGC AGGCGGTCAA GAGAGCGTTC ACCCGCACGC 
AAGCGTTCCA GAGACCGTTC TCCGAGAAGA GACCGCTCGC CACGACGGCC CCGTAGAGTG 
GTTCCTCGCT ACACTGTCCA GTTCTCCAAA TTTAGTTTGG ATAGCTCCAG CTGCGACATG 
ATGGAACTGC GACGGCGTTA TCAGAGCCTC TACATTCCCA GTGACTTTTT TGATGCCGTC 
TTCACCTGGG TGGATGCATT CCCTATGACC AGGCCCTTCC AGTTAAACAA TGCCTGCAAC 
TTCCACATCT TGCACAAAGA GGTGGATCCT TTAGTCAAGA ATACAGCTGT GCTGGACCCA 
CCTGATGCTA ACCACACCTA CAGTGCAAAG GTGATGCTGC TGGCCAACCC CAGTTTAGAG 
GAGCTTTATC ACAAATCCTG TGCTTTGGCG GAGGACACCC AGGAAGTCAA AGATTCCTTC 
CAGCATCCTG CTAGACTCAT TAAGTTTTTG GTGGGAATGA GAGGTAAAGA TGAGGCCATG 
GCCATTGGTG GTCACTGGTC CCCCTCTCTG GATGGAGCTG ACCCAGAGAG GGACCCGTCA 
GTCCTTATAA AGACAGCCAT ACGCTGTTGC AAGGCACTCA CAGGCATAGA CCTTAGTCTG 
TGCACTCAGT GGTATCGTTT TGCAGAGATT CGCTATCATC GACCAGAGGA GACACACAAG 
GGGCGGACAG TCCCTGCTCA TGTGGAGACA GTGGTTTTGT TTCTTCCGGA TGTTTGGCAT 
TGTCTTCCTA CCCGCTCAGA GTGGGAGGTG CTGTCACGGC GACTCCGGGA GCAGCTGGCT 
GAGAAGCTGT CGGCCGAGCG AAAGGAGGCG GATGGAGAAC AGGACGAAGA GGAGAAGGAC 
GACGGGGAAT CCAAGGACGT TAGTACTCCC ACGCACTGGG CTAAACTGGA CCCCAAATCA 
ATGAAGGTAA ACGACCTGCG CAAAGAGCTC GACTGTCGCT CCCTGAGCTC CAAAGGCTTG 
AAGTCGCAGC TGATCGCTCG CCTCACCAAA CAGCTGAAGG TGGAGGAGCA GGTGGAGGAG 
TCCAAGGAGC CAGACAAACC AGAGAGCAAA ATTCCTGAGG AGGAAGAGCC GTCTCGCACC 
GAAGAAGAGA AGGAGGAGGA GGAAAGGAAG CGCCAGGAGG AGCTTGACCG GCAAAGACGA 
GAGAGACGCT ACATCCTCCC CGATGAGCCC ACCATCCTCG TGAACCCCAA CTGGGCAGCA 
AAGAATGGCA AATTCGACTG CAGCGTCATG TCTCTGAGCG TGTTGCTGGA TTACCGACTG 
GAGGACAACA AGGAGCACTC GTTTGAGGTT TCTCTCTTTG CTGAGCTTTT TAATGAAATG 
CTCCAGAGAG ACTTTGGCTA CCGCATCTAT AAAGCCCTCG CTGCTATTCC TGTAAAAGAT 
GAGAAGAAGG AAAAGAAAAA CAAGAAGGAG GCTGAAAAAC GAGACGTGAA GAAGGAAAAG 
GAGGAGGAAA ACGAGGAGCC ACAGGCAAAG AAGGCTAAAG AGGACGAGGA GGACAAGAAG 
AAGAGCGAAG AAAAGACGCT GAAAAAGGAG CCCTCTCAAG ATGAAGAGGA GAACGAAGAT 
GAGGGCAGCA CGGCTAACGC TGAGGAGTAC GACCCAATGG AGGCGGAGGA CGCTGAAGAT 
GACGATGACG ACGACGACGA CAAGGACGAC GAGGACTCCA ACGACAGAGA CAGGAGAGAC 
CGCAGAGACG ACCGCAAGTC GTCGAGAGAG AGCGCCTCTA AAGATAAGGA GAAGAAGCAG 
ATGGTCACGC ACAACAGGGA GCTGCTCATG GCTTTCGTCT ACTTTGACCA GAGCCACTGT 
GGCTATTTGT TGGAACGGGA CCTGGAGGAG ATCTTGTACA CGTTGGGGCT GCACCTTTCA 
CGTGCACAGA TCAAGAAGCT GTTAAACAAA CCAGTGATCA GAGAATCTTG TTACTATCGA 
AAGCTGACGG ACAACGGGAA GGACGAACCT GTTCAGTCTA TGAATGAAGC TCAGATAGAA 
AACCTTACGG GTAACCGAAG TCTTCTTCCG GCTCCTAAAG CTCGCGTTAA GGCAGAGCCG 
AGCGAGTCGG GTAATCTGAT CGTATATAAC GGAGCCATGG TGGACGTTGG CAGCATGATG 
CAGAAACTGG AGAAGAGCGA GAAGACGCGC GAGGAGATCG AGCAGAAGCT AATGGTGCAG 
GATGCTAAGC TGGAAGAGGA CGCAAAAGTG AAAGCACAGC TGGAGAAGAG TAACAAGGCC 
TTGAACAGGG AGCTGGAGGA GGTGAAGAGG ACGCTGAATC AAACTGAGCA GACGCTGACG 
TCTGTGAAGG AGCAGAAGAC CGTCTACCAC GATCACATTG TCAAGACCTC CAACTCCTTG 
ATGTCCACGG TGAAAGGCCT GCTGGGACTG ATGAGCAAGA ATAAAGATGA GCACCTGGAC 
TTGGAAGTTG CTCTGGATCA GGCGTCACAA GCGAACGGCT CAGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029691036.1
CDS	123..3833
Translation	MAQFGGQKNPPWATQFAATAVSQPGHSGQSLDLNSLHSLGVQQPSLLGASPSVYPQQSALAAVSLSNQSAANYQLSQQTAALQQQAAAAAAAALQQSQINTALQQYQQQQQQQQQPPPQQPPQQQLYNVPHQLPQPQQALLSQPPVPLPTSMSLSNPQQTAQITVSYPTPRSSHQQQTQPQKQRVFTGVVNKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEAVYNPNMPFKWNAQRIQTLPQLPNQSHQQQPQPLPQASPQLGGLYNEPGMQLRYSDLHSAPDNRQNNQPQPPNMMKTAPAMLQSLPPPNTFSVPSQPPPPLLQAQLSAASLAPLLHNPPQPLLPQPPPKDVFAGGLLQPPVRMMSQPQPVRRIEPPPRFPNRNDRGPELILRSKEERSRDRDRERRRSRERSPARKRSRDRSPRRDRSPRRPRRVVPRYTVQFSKFSLDSSSCDMMELRRRYQSLYIPSDFFDAVFTWVDAFPMTRPFQLNNACNFHILHKEVDPLVKNTAVLDPPDANHTYSAKVMLLANPSLEELYHKSCALAEDTQEVKDSFQHPARLIKFLVGMRGKDEAMAIGGHWSPSLDGADPERDPSVLIKTAIRCCKALTGIDLSLCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFLPDVWHCLPTRSEWEVLSRRLREQLAEKLSAERKEADGEQDEEEKDDGESKDVSTPTHWAKLDPKSMKVNDLRKELDCRSLSSKGLKSQLIARLTKQLKVEEQVEESKEPDKPESKIPEEEEPSRTEEEKEEEERKRQEELDRQRRERRYILPDEPTILVNPNWAAKNGKFDCSVMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGYRIYKALAAIPVKDEKKEKKNKKEAEKRDVKKEKEEENEEPQAKKAKEDEEDKKKSEEKTLKKEPSQDEEENEDEGSTANAEEYDPMEAEDAEDDDDDDDDKDDEDSNDRDRRDRRDDRKSSRESASKDKEKKQMVTHNRELLMAFVYFDQSHCGYLLERDLEEILYTLGLHLSRAQIKKLLNKPVIRESCYYRKLTDNGKDEPVQSMNEAQIENLTGNRSLLPAPKARVKAEPSESGNLIVYNGAMVDVGSMMQKLEKSEKTREEIEQKLMVQDAKLEEDAKVKAQLEKSNKALNRELEEVKRTLNQTEQTLTSVKEQKTVYHDHIVKTSNSLMSTVKGLLGLMSKNKDEHLDLEVALDQASQANGSDE

Target ORF information:

RefSeq Version	XM_029835176.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029835176.1

ORF Insert Sequence:

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGC CCCCCGTGCC GTTGCCCACC AGCATGAGCT TGTCCAACCC TCAGCAGACA 
GCCCAAATTA CCGTGTCCTA CCCGACGCCG CGTTCGAGTC ACCAACAACA GACGCAGCCC 
CAGAAGCAGC GAGTCTTTAC TGGCGTCGTC AATAAGCTAC ACGACACGTT TGGCTTTGTA 
GATGAAGATG TTTTCTTTCA GCTGAGTGCG GTGAAGGGGA AGACTCCTCA GGTGGGTGAC 
AGAGTGCTCG TGGAAGCTGT GTACAACCCA AACATGCCGT TCAAGTGGAA TGCCCAACGG 
ATCCAAACCT TACCTCAGCT TCCAAACCAG TCGCATCAGC AGCAGCCTCA GCCTTTACCT 
CAAGCTTCCC CTCAGCTCGG CGGCCTTTAC AATGAACCAG GGATGCAGCT GCGCTACTCA 
GATCTTCACT CTGCTCCAGA CAACCGACAA AATAACCAGC CACAACCTCC TAATATGATG 
AAGACGGCCC CGGCCATGCT ACAATCCCTA CCTCCCCCAA ATACGTTCAG TGTCCCATCC 
CAGCCCCCTC CTCCACTTCT CCAAGCCCAG CTGTCTGCTG CCTCTTTGGC CCCTCTTCTG 
CACAACCCTC CCCAGCCTCT GCTCCCACAG CCTCCCCCCA AAGATGTCTT TGCCGGTGGT 
CTGCTTCAGC CCCCTGTCAG AATGATGTCA CAGCCCCAGC CTGTCCGGCG CATTGAACCC 
CCACCGCGTT TTCCCAATCG CAATGACCGG GGGCCTGAAC TCATCCTGAG AAGTAAAGAG 
GAACGCAGTC GGGACAGAGA CCGCGAGCGC AGGCGGTCAA GAGAGCGTTC ACCCGCACGC 
AAGCGTTCCA GAGACCGTTC TCCGAGAAGA GACCGCTCGC CACGACGGCC CCGTAGAGTG 
GTTCCTCGCT ACACTGTCCA GTTCTCCAAA TTTAGTTTGG ATAGCTCCAG CTGCGACATG 
ATGGAACTGC GACGGCGTTA TCAGAGCCTC TACATTCCCA GTGACTTTTT TGATGCCGTC 
TTCACCTGGG TGGATGCATT CCCTATGACC AGGCCCTTCC AGTTAAACAA TGCCTGCAAC 
TTCCACATCT TGCACAAAGA GGTGGATCCT TTAGTCAAGA ATACAGCTGT GCTGGACCCA 
CCTGATGCTA ACCACACCTA CAGTGCAAAG GTGATGCTGC TGGCCAACCC CAGTTTAGAG 
GAGCTTTATC ACAAATCCTG TGCTTTGGCG GAGGACACCC AGGAAGTCAA AGATTCCTTC 
CAGCATCCTG CTAGACTCAT TAAGTTTTTG GTGGGAATGA GAGGTAAAGA TGAGGCCATG 
GCCATTGGTG GTCACTGGTC CCCCTCTCTG GATGGAGCTG ACCCAGAGAG GGACCCGTCA 
GTCCTTATAA AGACAGCCAT ACGCTGTTGC AAGGCACTCA CAGGCATAGA CCTTAGTCTG 
TGCACTCAGT GGTATCGTTT TGCAGAGATT CGCTATCATC GACCAGAGGA GACACACAAG 
GGGCGGACAG TCCCTGCTCA TGTGGAGACA GTGGTTTTGT TTCTTCCGGA TGTTTGGCAT 
TGTCTTCCTA CCCGCTCAGA GTGGGAGGTG CTGTCACGGC GACTCCGGGA GCAGCTGGCT 
GAGAAGCTGT CGGCCGAGCG AAAGGAGGCG GATGGAGAAC AGGACGAAGA GGAGAAGGAC 
GACGGGGAAT CCAAGGACGT TAGTACTCCC ACGCACTGGG CTAAACTGGA CCCCAAATCA 
ATGAAGGTAA ACGACCTGCG CAAAGAGCTC GACTGTCGCT CCCTGAGCTC CAAAGGCTTG 
AAGTCGCAGC TGATCGCTCG CCTCACCAAA CAGCTGAAGG TGGAGGAGCA GGTGGAGGAG 
TCCAAGGAGC CAGACAAACC AGAGAGCAAA ATTCCTGAGG AGGAAGAGCC GTCTCGCACC 
GAAGAAGAGA AGGAGGAGGA GGAAAGGAAG CGCCAGGAGG AGCTTGACCG GCAAAGACGA 
GAGAGACGCT ACATCCTCCC CGATGAGCCC ACCATCCTCG TGAACCCCAA CTGGGCAGCA 
AAGAATGGCA AATTCGACTG CAGCGTCATG TCTCTGAGCG TGTTGCTGGA TTACCGACTG 
GAGGACAACA AGGAGCACTC GTTTGAGGTT TCTCTCTTTG CTGAGCTTTT TAATGAAATG 
CTCCAGAGAG ACTTTGGCTA CCGCATCTAT AAAGCCCTCG CTGCTATTCC TGTAAAAGAT 
GAGAAGAAGG AAAAGAAAAA CAAGAAGGAG GCTGAAAAAC GAGACGTGAA GAAGGAAAAG 
GAGGAGGAAA ACGAGGAGCC ACAGGCAAAG AAGGCTAAAG AGGACGAGGA GGACAAGAAG 
AAGAGCGAAG AAAAGACGCT GAAAAAGGAG CCCTCTCAAG ATGAAGAGGA GAACGAAGAT 
GAGGGCAGCA CGGCTAACGC TGAGGAGTAC GACCCAATGG AGGCGGAGGA CGCTGAAGAT 
GACGATGACG ACGACGACGA CAAGGACGAC GAGGACTCCA ACGACAGAGA CAGGAGAGAC 
CGCAGAGACG ACCGCAAGTC GTCGAGAGAG AGCGCCTCTA AAGATAAGGA GAAGAAGCAG 
ATGGTCACGC ACAACAGGGA GCTGCTCATG GCTTTCGTCT ACTTTGACCA GAGCCACTGT 
GGCTATTTGT TGGAACGGGA CCTGGAGGAG ATCTTGTACA CGTTGGGGCT GCACCTTTCA 
CGTGCACAGA TCAAGAAGCT GTTAAACAAA CCAGTGATCA GAGAATCTTG TTACTATCGA 
AAGCTGACGG ACAACGGGAA GGACGAACCT GTTCAGTCTA TGAATGAAGC TCAGATAGAA 
AACCTTACGG GTAACCGAAG TCTTCTTCCG GCTCCTAAAG CTCGCGTTAA GGCAGAGCCG 
AGCGAGTCGG GTAATCTGAT CGTATATAAC GGAGCCATGG TGGACGTTGG CAGCATGATG 
CAGAAACTGG AGAAGAGCGA GAAGACGCGC GAGGAGATCG AGCAGAAGCT AATGGTGCAG 
GATGCTAAGC TGGAAGAGGA CGCAAAAGTG AAAGCACAGC TGGAGAAGAG TAACAAGGCC 
TTGAACAGGG AGCTGGAGGA GGTGAAGAGG ACGCTGAATC AAACTGAGCA GACGCTGACG 
TCTGTGAAGG AGCAGAAGAC CGTCTACCAC GATCACATTG TCAAGACCTC CAACTCCTTG 
ATGTCCACGG TGAAAGGCCT GCTGGGACTG ATGAGCAAGA ATAAAGATGA GCACCTGGAC 
TTGGAAGTTG CTCTGGATCA GGCGTCACAA GCGAACGGCT CAGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccar1 ( XM_011603318.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601620.1 Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo29840
Clone ID Related Accession (Same CDS sequence)	XM_011603318.1 , XM_011603317.1
Accession Version	XM_011603318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	cell division cycle and apoptosis regulator protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070947.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCACG TTCAAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC CGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGTGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601620.1
CDS	113..3841
Translation	MAQFGGQKNPPWATQFAATAVSQPGHSGQSLDLNSLHSLGVQQPSLLGASPSVYPQQSALAAVSLSNQSAANYQLSQQTAALQQQAAAAAAAALQQSQINTALQQYQQQQQQQQQPPPQQPPQQQLYNVPHQLPQPQQALLSQFIPDPQPPVPLPTSMSLSNPQQTAQITVSYPTPRSSHQQQTQPQKQRVFTGVVNKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEAVYNPNMPFKWNAQRIQTLPQLPNQSHQQQPQPLPQASPQLGGLYNEPGMQLRYSDLHSAPDNRQNNQPQPPNMMKTAPAMLQSLPPPNTFSVPSQPPPPLLQAQLSAASLAPLLHNPPQPLLPQPPPKDVFAGGLLQPPVRMMSQPQPVRRIEPPPRFPNRNDRGPELILRSKEERSRDRDRERRRSRERSPARKRSRDRSPRRDRSPRRPRRVVPRYTVQFSKFSLDSSSCDMMELRRRYQSLYIPSDFFDAVFTWVDAFPMTRPFQLNNACNFHILHKEVDPLVKNTAVLDPPDANHTYSAKVMLLANPSLEELYHKSCALAEDTQEVKDSFQHPARLIKFLVGMRGKDEAMAIGGHWSPSLDGADPERDPSVLIKTAIRCCKALTGIDLSLCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFLPDVWHCLPTRSEWEVLSRRLREQLAEKLSAERKEADGEQDEEEKDDGESKDVSTPTHWAKLDPKSMKVNDLRKELDCRSLSSKGLKSQLIARLTKQLKVEEQVEESKEPDKPESKIPEEEEPSRTEEEKEEEERKRQEELDRQRRERRYILPDEPTILVNPNWAAKNGKFDCSVMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGYRIYKALAAIPVKDEKKEKKNKKEAEKRDVKKEKEEENEEPQAKKAKEDEEDKKKSEEKTLKKEPSQDEEENEDEGSTANAEEYDPMEAEDAEDDDDDDDDKDDEDSNDRDRRDRRDDRKSSRESASKDKEKKQMVTHNRELLMAFVYFDQSHCGYLLERDLEEILYTLGLHLSRAQIKKLLNKPVIRESCYYRKLTDNGKDEPVQSMNEAQIENLTGNRSLLPAPKARVKAEPSESGNLIVYNGAMVDVGSMMQKLEKSEKTREEIEQKLMVQDAKLEEDAKVKAQLEKSNKALNRELEEVKRTLNQTEQTLTSVKEQKTVYHDHIVKTSNSLMSTVKGLLGLMSKNKDEHLDLEVALDQASQANGSDE

Target ORF information:

RefSeq Version	XM_011603318.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603318.1

ORF Insert Sequence:

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCACG TTCAAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC CGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGTGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccar1 ( XM_011603317.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601619.1 Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo29840
Clone ID Related Accession (Same CDS sequence)	XM_011603318.1 , XM_011603317.1
Accession Version	XM_011603317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	cell division cycle and apoptosis regulator protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070947.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCACG TTCAAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC CGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGTGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601619.1
CDS	331..4059
Translation	MAQFGGQKNPPWATQFAATAVSQPGHSGQSLDLNSLHSLGVQQPSLLGASPSVYPQQSALAAVSLSNQSAANYQLSQQTAALQQQAAAAAAAALQQSQINTALQQYQQQQQQQQQPPPQQPPQQQLYNVPHQLPQPQQALLSQFIPDPQPPVPLPTSMSLSNPQQTAQITVSYPTPRSSHQQQTQPQKQRVFTGVVNKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEAVYNPNMPFKWNAQRIQTLPQLPNQSHQQQPQPLPQASPQLGGLYNEPGMQLRYSDLHSAPDNRQNNQPQPPNMMKTAPAMLQSLPPPNTFSVPSQPPPPLLQAQLSAASLAPLLHNPPQPLLPQPPPKDVFAGGLLQPPVRMMSQPQPVRRIEPPPRFPNRNDRGPELILRSKEERSRDRDRERRRSRERSPARKRSRDRSPRRDRSPRRPRRVVPRYTVQFSKFSLDSSSCDMMELRRRYQSLYIPSDFFDAVFTWVDAFPMTRPFQLNNACNFHILHKEVDPLVKNTAVLDPPDANHTYSAKVMLLANPSLEELYHKSCALAEDTQEVKDSFQHPARLIKFLVGMRGKDEAMAIGGHWSPSLDGADPERDPSVLIKTAIRCCKALTGIDLSLCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFLPDVWHCLPTRSEWEVLSRRLREQLAEKLSAERKEADGEQDEEEKDDGESKDVSTPTHWAKLDPKSMKVNDLRKELDCRSLSSKGLKSQLIARLTKQLKVEEQVEESKEPDKPESKIPEEEEPSRTEEEKEEEERKRQEELDRQRRERRYILPDEPTILVNPNWAAKNGKFDCSVMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGYRIYKALAAIPVKDEKKEKKNKKEAEKRDVKKEKEEENEEPQAKKAKEDEEDKKKSEEKTLKKEPSQDEEENEDEGSTANAEEYDPMEAEDAEDDDDDDDDKDDEDSNDRDRRDRRDDRKSSRESASKDKEKKQMVTHNRELLMAFVYFDQSHCGYLLERDLEEILYTLGLHLSRAQIKKLLNKPVIRESCYYRKLTDNGKDEPVQSMNEAQIENLTGNRSLLPAPKARVKAEPSESGNLIVYNGAMVDVGSMMQKLEKSEKTREEIEQKLMVQDAKLEEDAKVKAQLEKSNKALNRELEEVKRTLNQTEQTLTSVKEQKTVYHDHIVKTSNSLMSTVKGLLGLMSKNKDEHLDLEVALDQASQANGSDE

Target ORF information:

RefSeq Version	XM_011603317.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603317.1

ORF Insert Sequence:

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGT TTATTCCTGA CCCTCAGCCC CCCGTGCCGT TGCCCACCAG CATGAGCTTG 
TCCAACCCTC AGCAGACAGC CCAAATTACC GTGTCCTACC CGACGCCACG TTCAAGTCAC 
CAACAACAGA CGCAGCCCCA GAAGCAGCGA GTCTTTACTG GCGTCGTCAA TAAGCTACAC 
GACACGTTTG GCTTTGTAGA TGAAGATGTT TTCTTTCAGC TGAGTGCGGT GAAGGGGAAG 
ACTCCTCAGG TGGGTGACAG AGTGCTCGTG GAAGCTGTGT ACAACCCAAA CATGCCGTTC 
AAGTGGAATG CCCAACGGAT CCAAACCTTA CCTCAGCTTC CAAACCAGTC GCATCAGCAG 
CAGCCTCAGC CTTTACCTCA AGCTTCCCCT CAGCTCGGCG GCCTTTACAA TGAACCAGGG 
ATGCAGCTGC GCTACTCAGA TCTTCACTCT GCTCCAGACA ACCGACAAAA TAACCAGCCA 
CAACCTCCTA ATATGATGAA GACGGCCCCG GCCATGCTAC AATCCCTACC TCCCCCAAAT 
ACGTTCAGTG TCCCATCCCA GCCCCCTCCT CCACTTCTCC AAGCCCAGCT GTCTGCTGCC 
TCTTTGGCCC CTCTTCTGCA CAACCCTCCC CAGCCTCTGC TCCCACAGCC TCCCCCCAAA 
GATGTCTTTG CCGGTGGTCT GCTTCAGCCC CCTGTCAGAA TGATGTCACA GCCCCAGCCT 
GTCCGGCGCA TTGAACCCCC ACCGCGTTTT CCCAATCGCA ATGACCGGGG GCCTGAACTC 
ATCCTGAGAA GTAAAGAGGA ACGCAGTCGG GACAGAGACC GCGAGCGCAG GCGGTCAAGA 
GAGCGTTCAC CCGCACGCAA GCGTTCCAGA GACCGTTCTC CGAGAAGAGA CCGCTCGCCA 
CGACGGCCCC GTAGAGTGGT TCCTCGCTAC ACTGTCCAGT TCTCCAAATT TAGTTTGGAT 
AGCTCCAGCT GCGACATGAT GGAACTGCGA CGGCGTTATC AGAGCCTCTA CATTCCCAGT 
GACTTTTTTG ATGCCGTCTT CACCTGGGTG GATGCATTCC CTATGACCAG GCCCTTCCAG 
TTAAACAATG CCTGCAACTT CCACATCTTG CACAAAGAGG TGGATCCTTT AGTCAAGAAT 
ACAGCTGTGC TGGACCCACC TGATGCTAAC CACACCTACA GTGCAAAGGT GATGCTGCTG 
GCCAACCCCA GTTTAGAGGA GCTTTATCAC AAATCCTGTG CTTTGGCGGA GGACACCCAG 
GAAGTCAAAG ATTCCTTCCA GCATCCTGCT AGACTCATTA AGTTTTTGGT GGGAATGAGA 
GGTAAAGATG AGGCCATGGC CATTGGTGGT CACTGGTCCC CCTCTCTGGA TGGAGCTGAC 
CCAGAGAGGG ACCCGTCAGT CCTTATAAAG ACAGCCATAC GCTGTTGCAA GGCACTCACA 
GGCATAGACC TTAGTCTGTG CACTCAGTGG TATCGTTTTG CAGAGATTCG CTATCATCGA 
CCAGAGGAGA CACACAAGGG GCGGACAGTC CCTGCTCATG TGGAGACAGT GGTTTTGTTT 
CTTCCGGATG TTTGGCATTG TCTTCCTACC CGCTCAGAGT GGGAGGTGCT GTCACGGCGA 
CTCCGGGAGC AGCTGGCTGA GAAGCTGTCG GCCGAGCGAA AGGAGGCGGA TGGAGAACAG 
GACGAAGAGG AGAAGGACGA CGGGGAATCC AAGGACGTTA GTACTCCCAC GCACTGGGCT 
AAACTGGACC CCAAATCAAT GAAGGTAAAC GACCTGCGCA AAGAGCTCGA CTGTCGCTCC 
CTGAGCTCCA AAGGCTTGAA GTCGCAGCTG ATCGCTCGCC TCACCAAACA GCTGAAGGTG 
GAGGAGCAGG TGGAGGAGTC CAAGGAGCCA GACAAACCAG AGAGCAAAAT TCCTGAGGAG 
GAAGAGCCGT CTCGCACCGA AGAAGAGAAG GAGGAGGAGG AAAGGAAGCG CCAGGAGGAG 
CTTGACCGGC AAAGACGAGA GAGACGCTAC ATCCTCCCCG ATGAGCCCAC CATCCTCGTG 
AACCCCAACT GGGCAGCAAA GAATGGCAAA TTCGACTGCA GCGTCATGTC TCTGAGCGTG 
TTGCTGGATT ACCGACTGGA GGACAACAAG GAGCACTCGT TTGAGGTTTC TCTCTTTGCT 
GAGCTTTTTA ATGAAATGCT CCAGAGAGAC TTTGGCTACC GCATCTATAA AGCCCTCGCT 
GCTATTCCTG TAAAAGATGA GAAGAAGGAA AAGAAAAACA AGAAGGAGGC TGAAAAACGA 
GACGTGAAGA AGGAAAAGGA GGAGGAAAAC GAGGAGCCAC AGGCAAAGAA GGCTAAAGAG 
GACGAGGAGG ACAAGAAGAA GAGCGAAGAA AAGACGCTGA AAAAGGAGCC CTCTCAAGAT 
GAAGAGGAGA ACGAAGATGA GGGCAGCACG GCTAACGCTG AGGAGTACGA CCCAATGGAG 
GCGGAGGACG CTGAAGATGA CGATGACGAC GACGACGACA AGGACGACGA GGACTCCAAC 
GACAGAGACA GGAGAGACCG CAGAGACGAC CGCAAGTCGT CGAGAGAGAG CGCCTCTAAA 
GATAAGGAGA AGAAGCAGAT GGTCACGCAC AACAGGGAGC TGCTCATGGC TTTCGTCTAC 
TTTGACCAGA GCCACTGTGG CTATTTGTTG GAACGGGACC TGGAGGAGAT CTTGTACACG 
TTGGGGCTGC ACCTTTCACG TGCACAGATC AAGAAGCTGT TAAACAAACC CGTGATCAGA 
GAATCTTGTT ACTATCGAAA GCTGACGGAC AACGGGAAGG ACGAACCTGT TCAGTCTATG 
AATGAAGCTC AGATAGAAAA CCTTACGGGT AACCGAAGTC TTCTTCCGGC TCCTAAAGCT 
CGTGTTAAGG CAGAGCCGAG CGAGTCGGGT AATCTGATCG TATATAACGG AGCCATGGTG 
GACGTTGGCA GCATGATGCA GAAACTGGAG AAGAGCGAGA AGACGCGCGA GGAGATCGAG 
CAGAAGCTAA TGGTGCAGGA TGCTAAGCTG GAAGAGGACG CAAAAGTGAA AGCACAGCTG 
GAGAAGAGTA ACAAGGCCTT GAACAGGGAG CTGGAGGAGG TGAAGAGGAC GCTGAATCAA 
ACTGAGCAGA CGCTGACGTC TGTGAAGGAG CAGAAGACCG TCTACCACGA TCACATTGTC 
AAGACCTCCA ACTCCTTGAT GTCCACGGTG AAAGGCCTGC TGGGACTGAT GAGCAAGAAT 
AAAGATGAGC ACCTGGACTT GGAAGTTGCT CTGGATCAGG CGTCACAAGC GAACGGCTCA 
GATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ccar1 ( XM_011603319.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011601621.1 Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X3, mRNA.

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CloneID	OTo29841
Clone ID Related Accession (Same CDS sequence)	XM_011603319.1
Accession Version	XM_011603319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	cell division cycle and apoptosis regulator protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070947.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGC CCCCCGTGCC GTTGCCCACC AGCATGAGCT TGTCCAACCC TCAGCAGACA 
GCCCAAATTA CCGTGTCCTA CCCGACGCCA CGTTCAAGTC ACCAACAACA GACGCAGCCC 
CAGAAGCAGC GAGTCTTTAC TGGCGTCGTC AATAAGCTAC ACGACACGTT TGGCTTTGTA 
GATGAAGATG TTTTCTTTCA GCTGAGTGCG GTGAAGGGGA AGACTCCTCA GGTGGGTGAC 
AGAGTGCTCG TGGAAGCTGT GTACAACCCA AACATGCCGT TCAAGTGGAA TGCCCAACGG 
ATCCAAACCT TACCTCAGCT TCCAAACCAG TCGCATCAGC AGCAGCCTCA GCCTTTACCT 
CAAGCTTCCC CTCAGCTCGG CGGCCTTTAC AATGAACCAG GGATGCAGCT GCGCTACTCA 
GATCTTCACT CTGCTCCAGA CAACCGACAA AATAACCAGC CACAACCTCC TAATATGATG 
AAGACGGCCC CGGCCATGCT ACAATCCCTA CCTCCCCCAA ATACGTTCAG TGTCCCATCC 
CAGCCCCCTC CTCCACTTCT CCAAGCCCAG CTGTCTGCTG CCTCTTTGGC CCCTCTTCTG 
CACAACCCTC CCCAGCCTCT GCTCCCACAG CCTCCCCCCA AAGATGTCTT TGCCGGTGGT 
CTGCTTCAGC CCCCTGTCAG AATGATGTCA CAGCCCCAGC CTGTCCGGCG CATTGAACCC 
CCACCGCGTT TTCCCAATCG CAATGACCGG GGGCCTGAAC TCATCCTGAG AAGTAAAGAG 
GAACGCAGTC GGGACAGAGA CCGCGAGCGC AGGCGGTCAA GAGAGCGTTC ACCCGCACGC 
AAGCGTTCCA GAGACCGTTC TCCGAGAAGA GACCGCTCGC CACGACGGCC CCGTAGAGTG 
GTTCCTCGCT ACACTGTCCA GTTCTCCAAA TTTAGTTTGG ATAGCTCCAG CTGCGACATG 
ATGGAACTGC GACGGCGTTA TCAGAGCCTC TACATTCCCA GTGACTTTTT TGATGCCGTC 
TTCACCTGGG TGGATGCATT CCCTATGACC AGGCCCTTCC AGTTAAACAA TGCCTGCAAC 
TTCCACATCT TGCACAAAGA GGTGGATCCT TTAGTCAAGA ATACAGCTGT GCTGGACCCA 
CCTGATGCTA ACCACACCTA CAGTGCAAAG GTGATGCTGC TGGCCAACCC CAGTTTAGAG 
GAGCTTTATC ACAAATCCTG TGCTTTGGCG GAGGACACCC AGGAAGTCAA AGATTCCTTC 
CAGCATCCTG CTAGACTCAT TAAGTTTTTG GTGGGAATGA GAGGTAAAGA TGAGGCCATG 
GCCATTGGTG GTCACTGGTC CCCCTCTCTG GATGGAGCTG ACCCAGAGAG GGACCCGTCA 
GTCCTTATAA AGACAGCCAT ACGCTGTTGC AAGGCACTCA CAGGCATAGA CCTTAGTCTG 
TGCACTCAGT GGTATCGTTT TGCAGAGATT CGCTATCATC GACCAGAGGA GACACACAAG 
GGGCGGACAG TCCCTGCTCA TGTGGAGACA GTGGTTTTGT TTCTTCCGGA TGTTTGGCAT 
TGTCTTCCTA CCCGCTCAGA GTGGGAGGTG CTGTCACGGC GACTCCGGGA GCAGCTGGCT 
GAGAAGCTGT CGGCCGAGCG AAAGGAGGCG GATGGAGAAC AGGACGAAGA GGAGAAGGAC 
GACGGGGAAT CCAAGGACGT TAGTACTCCC ACGCACTGGG CTAAACTGGA CCCCAAATCA 
ATGAAGGTAA ACGACCTGCG CAAAGAGCTC GACTGTCGCT CCCTGAGCTC CAAAGGCTTG 
AAGTCGCAGC TGATCGCTCG CCTCACCAAA CAGCTGAAGG TGGAGGAGCA GGTGGAGGAG 
TCCAAGGAGC CAGACAAACC AGAGAGCAAA ATTCCTGAGG AGGAAGAGCC GTCTCGCACC 
GAAGAAGAGA AGGAGGAGGA GGAAAGGAAG CGCCAGGAGG AGCTTGACCG GCAAAGACGA 
GAGAGACGCT ACATCCTCCC CGATGAGCCC ACCATCCTCG TGAACCCCAA CTGGGCAGCA 
AAGAATGGCA AATTCGACTG CAGCGTCATG TCTCTGAGCG TGTTGCTGGA TTACCGACTG 
GAGGACAACA AGGAGCACTC GTTTGAGGTT TCTCTCTTTG CTGAGCTTTT TAATGAAATG 
CTCCAGAGAG ACTTTGGCTA CCGCATCTAT AAAGCCCTCG CTGCTATTCC TGTAAAAGAT 
GAGAAGAAGG AAAAGAAAAA CAAGAAGGAG GCTGAAAAAC GAGACGTGAA GAAGGAAAAG 
GAGGAGGAAA ACGAGGAGCC ACAGGCAAAG AAGGCTAAAG AGGACGAGGA GGACAAGAAG 
AAGAGCGAAG AAAAGACGCT GAAAAAGGAG CCCTCTCAAG ATGAAGAGGA GAACGAAGAT 
GAGGGCAGCA CGGCTAACGC TGAGGAGTAC GACCCAATGG AGGCGGAGGA CGCTGAAGAT 
GACGATGACG ACGACGACGA CAAGGACGAC GAGGACTCCA ACGACAGAGA CAGGAGAGAC 
CGCAGAGACG ACCGCAAGTC GTCGAGAGAG AGCGCCTCTA AAGATAAGGA GAAGAAGCAG 
ATGGTCACGC ACAACAGGGA GCTGCTCATG GCTTTCGTCT ACTTTGACCA GAGCCACTGT 
GGCTATTTGT TGGAACGGGA CCTGGAGGAG ATCTTGTACA CGTTGGGGCT GCACCTTTCA 
CGTGCACAGA TCAAGAAGCT GTTAAACAAA CCCGTGATCA GAGAATCTTG TTACTATCGA 
AAGCTGACGG ACAACGGGAA GGACGAACCT GTTCAGTCTA TGAATGAAGC TCAGATAGAA 
AACCTTACGG GTAACCGAAG TCTTCTTCCG GCTCCTAAAG CTCGTGTTAA GGCAGAGCCG 
AGCGAGTCGG GTAATCTGAT CGTATATAAC GGAGCCATGG TGGACGTTGG CAGCATGATG 
CAGAAACTGG AGAAGAGCGA GAAGACGCGC GAGGAGATCG AGCAGAAGCT AATGGTGCAG 
GATGCTAAGC TGGAAGAGGA CGCAAAAGTG AAAGCACAGC TGGAGAAGAG TAACAAGGCC 
TTGAACAGGG AGCTGGAGGA GGTGAAGAGG ACGCTGAATC AAACTGAGCA GACGCTGACG 
TCTGTGAAGG AGCAGAAGAC CGTCTACCAC GATCACATTG TCAAGACCTC CAACTCCTTG 
ATGTCCACGG TGAAAGGCCT GCTGGGACTG ATGAGCAAGA ATAAAGATGA GCACCTGGAC 
TTGGAAGTTG CTCTGGATCA GGCGTCACAA GCGAACGGCT CAGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011601621.1
CDS	331..4041
Translation	MAQFGGQKNPPWATQFAATAVSQPGHSGQSLDLNSLHSLGVQQPSLLGASPSVYPQQSALAAVSLSNQSAANYQLSQQTAALQQQAAAAAAAALQQSQINTALQQYQQQQQQQQQPPPQQPPQQQLYNVPHQLPQPQQALLSQPPVPLPTSMSLSNPQQTAQITVSYPTPRSSHQQQTQPQKQRVFTGVVNKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEAVYNPNMPFKWNAQRIQTLPQLPNQSHQQQPQPLPQASPQLGGLYNEPGMQLRYSDLHSAPDNRQNNQPQPPNMMKTAPAMLQSLPPPNTFSVPSQPPPPLLQAQLSAASLAPLLHNPPQPLLPQPPPKDVFAGGLLQPPVRMMSQPQPVRRIEPPPRFPNRNDRGPELILRSKEERSRDRDRERRRSRERSPARKRSRDRSPRRDRSPRRPRRVVPRYTVQFSKFSLDSSSCDMMELRRRYQSLYIPSDFFDAVFTWVDAFPMTRPFQLNNACNFHILHKEVDPLVKNTAVLDPPDANHTYSAKVMLLANPSLEELYHKSCALAEDTQEVKDSFQHPARLIKFLVGMRGKDEAMAIGGHWSPSLDGADPERDPSVLIKTAIRCCKALTGIDLSLCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFLPDVWHCLPTRSEWEVLSRRLREQLAEKLSAERKEADGEQDEEEKDDGESKDVSTPTHWAKLDPKSMKVNDLRKELDCRSLSSKGLKSQLIARLTKQLKVEEQVEESKEPDKPESKIPEEEEPSRTEEEKEEEERKRQEELDRQRRERRYILPDEPTILVNPNWAAKNGKFDCSVMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGYRIYKALAAIPVKDEKKEKKNKKEAEKRDVKKEKEEENEEPQAKKAKEDEEDKKKSEEKTLKKEPSQDEEENEDEGSTANAEEYDPMEAEDAEDDDDDDDDKDDEDSNDRDRRDRRDDRKSSRESASKDKEKKQMVTHNRELLMAFVYFDQSHCGYLLERDLEEILYTLGLHLSRAQIKKLLNKPVIRESCYYRKLTDNGKDEPVQSMNEAQIENLTGNRSLLPAPKARVKAEPSESGNLIVYNGAMVDVGSMMQKLEKSEKTREEIEQKLMVQDAKLEEDAKVKAQLEKSNKALNRELEEVKRTLNQTEQTLTSVKEQKTVYHDHIVKTSNSLMSTVKGLLGLMSKNKDEHLDLEVALDQASQANGSDE

Target ORF information:

RefSeq Version	XM_011603319.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes cell division cycle and apoptosis regulator 1 (ccar1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011603319.1

ORF Insert Sequence:

ATGGCCCAGT TTGGGGGGCA GAAGAACCCA CCCTGGGCGA CTCAGTTTGC TGCCACAGCG 
GTGTCCCAGC CGGGTCACTC GGGGCAGTCT CTGGATCTCA ACAGCCTGCA TTCTTTAGGT 
GTGCAGCAGC CGTCTCTTCT AGGAGCCTCT CCGTCTGTTT ATCCCCAGCA GTCGGCCTTA 
GCTGCAGTTT CCCTCAGCAA CCAGTCGGCT GCAAACTACC AGCTGTCTCA GCAAACCGCT 
GCGCTGCAAC AGCAGGCAGC AGCTGCCGCT GCTGCAGCGC TACAGCAGTC TCAGATTAAC 
ACGGCACTCC AGCAGTATCA GCAGCAGCAG CAGCAGCAAC AGCAGCCGCC TCCCCAGCAG 
CCCCCCCAAC AGCAACTGTA CAATGTACCT CATCAGCTTC CACAGCCTCA GCAAGCACTA 
CTTTCTCAGC CCCCCGTGCC GTTGCCCACC AGCATGAGCT TGTCCAACCC TCAGCAGACA 
GCCCAAATTA CCGTGTCCTA CCCGACGCCA CGTTCAAGTC ACCAACAACA GACGCAGCCC 
CAGAAGCAGC GAGTCTTTAC TGGCGTCGTC AATAAGCTAC ACGACACGTT TGGCTTTGTA 
GATGAAGATG TTTTCTTTCA GCTGAGTGCG GTGAAGGGGA AGACTCCTCA GGTGGGTGAC 
AGAGTGCTCG TGGAAGCTGT GTACAACCCA AACATGCCGT TCAAGTGGAA TGCCCAACGG 
ATCCAAACCT TACCTCAGCT TCCAAACCAG TCGCATCAGC AGCAGCCTCA GCCTTTACCT 
CAAGCTTCCC CTCAGCTCGG CGGCCTTTAC AATGAACCAG GGATGCAGCT GCGCTACTCA 
GATCTTCACT CTGCTCCAGA CAACCGACAA AATAACCAGC CACAACCTCC TAATATGATG 
AAGACGGCCC CGGCCATGCT ACAATCCCTA CCTCCCCCAA ATACGTTCAG TGTCCCATCC 
CAGCCCCCTC CTCCACTTCT CCAAGCCCAG CTGTCTGCTG CCTCTTTGGC CCCTCTTCTG 
CACAACCCTC CCCAGCCTCT GCTCCCACAG CCTCCCCCCA AAGATGTCTT TGCCGGTGGT 
CTGCTTCAGC CCCCTGTCAG AATGATGTCA CAGCCCCAGC CTGTCCGGCG CATTGAACCC 
CCACCGCGTT TTCCCAATCG CAATGACCGG GGGCCTGAAC TCATCCTGAG AAGTAAAGAG 
GAACGCAGTC GGGACAGAGA CCGCGAGCGC AGGCGGTCAA GAGAGCGTTC ACCCGCACGC 
AAGCGTTCCA GAGACCGTTC TCCGAGAAGA GACCGCTCGC CACGACGGCC CCGTAGAGTG 
GTTCCTCGCT ACACTGTCCA GTTCTCCAAA TTTAGTTTGG ATAGCTCCAG CTGCGACATG 
ATGGAACTGC GACGGCGTTA TCAGAGCCTC TACATTCCCA GTGACTTTTT TGATGCCGTC 
TTCACCTGGG TGGATGCATT CCCTATGACC AGGCCCTTCC AGTTAAACAA TGCCTGCAAC 
TTCCACATCT TGCACAAAGA GGTGGATCCT TTAGTCAAGA ATACAGCTGT GCTGGACCCA 
CCTGATGCTA ACCACACCTA CAGTGCAAAG GTGATGCTGC TGGCCAACCC CAGTTTAGAG 
GAGCTTTATC ACAAATCCTG TGCTTTGGCG GAGGACACCC AGGAAGTCAA AGATTCCTTC 
CAGCATCCTG CTAGACTCAT TAAGTTTTTG GTGGGAATGA GAGGTAAAGA TGAGGCCATG 
GCCATTGGTG GTCACTGGTC CCCCTCTCTG GATGGAGCTG ACCCAGAGAG GGACCCGTCA 
GTCCTTATAA AGACAGCCAT ACGCTGTTGC AAGGCACTCA CAGGCATAGA CCTTAGTCTG 
TGCACTCAGT GGTATCGTTT TGCAGAGATT CGCTATCATC GACCAGAGGA GACACACAAG 
GGGCGGACAG TCCCTGCTCA TGTGGAGACA GTGGTTTTGT TTCTTCCGGA TGTTTGGCAT 
TGTCTTCCTA CCCGCTCAGA GTGGGAGGTG CTGTCACGGC GACTCCGGGA GCAGCTGGCT 
GAGAAGCTGT CGGCCGAGCG AAAGGAGGCG GATGGAGAAC AGGACGAAGA GGAGAAGGAC 
GACGGGGAAT CCAAGGACGT TAGTACTCCC ACGCACTGGG CTAAACTGGA CCCCAAATCA 
ATGAAGGTAA ACGACCTGCG CAAAGAGCTC GACTGTCGCT CCCTGAGCTC CAAAGGCTTG 
AAGTCGCAGC TGATCGCTCG CCTCACCAAA CAGCTGAAGG TGGAGGAGCA GGTGGAGGAG 
TCCAAGGAGC CAGACAAACC AGAGAGCAAA ATTCCTGAGG AGGAAGAGCC GTCTCGCACC 
GAAGAAGAGA AGGAGGAGGA GGAAAGGAAG CGCCAGGAGG AGCTTGACCG GCAAAGACGA 
GAGAGACGCT ACATCCTCCC CGATGAGCCC ACCATCCTCG TGAACCCCAA CTGGGCAGCA 
AAGAATGGCA AATTCGACTG CAGCGTCATG TCTCTGAGCG TGTTGCTGGA TTACCGACTG 
GAGGACAACA AGGAGCACTC GTTTGAGGTT TCTCTCTTTG CTGAGCTTTT TAATGAAATG 
CTCCAGAGAG ACTTTGGCTA CCGCATCTAT AAAGCCCTCG CTGCTATTCC TGTAAAAGAT 
GAGAAGAAGG AAAAGAAAAA CAAGAAGGAG GCTGAAAAAC GAGACGTGAA GAAGGAAAAG 
GAGGAGGAAA ACGAGGAGCC ACAGGCAAAG AAGGCTAAAG AGGACGAGGA GGACAAGAAG 
AAGAGCGAAG AAAAGACGCT GAAAAAGGAG CCCTCTCAAG ATGAAGAGGA GAACGAAGAT 
GAGGGCAGCA CGGCTAACGC TGAGGAGTAC GACCCAATGG AGGCGGAGGA CGCTGAAGAT 
GACGATGACG ACGACGACGA CAAGGACGAC GAGGACTCCA ACGACAGAGA CAGGAGAGAC 
CGCAGAGACG ACCGCAAGTC GTCGAGAGAG AGCGCCTCTA AAGATAAGGA GAAGAAGCAG 
ATGGTCACGC ACAACAGGGA GCTGCTCATG GCTTTCGTCT ACTTTGACCA GAGCCACTGT 
GGCTATTTGT TGGAACGGGA CCTGGAGGAG ATCTTGTACA CGTTGGGGCT GCACCTTTCA 
CGTGCACAGA TCAAGAAGCT GTTAAACAAA CCCGTGATCA GAGAATCTTG TTACTATCGA 
AAGCTGACGG ACAACGGGAA GGACGAACCT GTTCAGTCTA TGAATGAAGC TCAGATAGAA 
AACCTTACGG GTAACCGAAG TCTTCTTCCG GCTCCTAAAG CTCGTGTTAA GGCAGAGCCG 
AGCGAGTCGG GTAATCTGAT CGTATATAAC GGAGCCATGG TGGACGTTGG CAGCATGATG 
CAGAAACTGG AGAAGAGCGA GAAGACGCGC GAGGAGATCG AGCAGAAGCT AATGGTGCAG 
GATGCTAAGC TGGAAGAGGA CGCAAAAGTG AAAGCACAGC TGGAGAAGAG TAACAAGGCC 
TTGAACAGGG AGCTGGAGGA GGTGAAGAGG ACGCTGAATC AAACTGAGCA GACGCTGACG 
TCTGTGAAGG AGCAGAAGAC CGTCTACCAC GATCACATTG TCAAGACCTC CAACTCCTTG 
ATGTCCACGG TGAAAGGCCT GCTGGGACTG ATGAGCAAGA ATAAAGATGA GCACCTGGAC 
TTGGAAGTTG CTCTGGATCA GGCGTCACAA GCGAACGGCT CAGATGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ccar1 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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