LOC101075355 gene cDNA ORF clone, Takifugu rubripes(torafugu)

mRNA	Protein	Name
XM_003961874.2	XP_003961923.1	E3 ubiquitin-protein ligase Midline-1-like isoform X1
XM_011609188.1	XP_011607490.1	E3 ubiquitin-protein ligase Midline-1-like isoform X1
XM_011609195.1	XP_011607497.1	E3 ubiquitin-protein ligase Midline-1-like isoform X1
XM_029843803.1	XP_029699663.1	E3 ubiquitin-protein ligase Midline-1-like
XM_011609188.2	XP_011607490.2	E3 ubiquitin-protein ligase Midline-1-like
XM_011609176.2	XP_011607478.2	E3 ubiquitin-protein ligase Midline-1-like
XM_029843807.1	XP_029699667.1	E3 ubiquitin-protein ligase Midline-1-like
XM_011609195.2	XP_011607497.2	E3 ubiquitin-protein ligase Midline-1-like
XM_011609176.1	XP_011607478.1	E3 ubiquitin-protein ligase Midline-1-like isoform X1
XM_011609200.1	XP_011607502.1	E3 ubiquitin-protein ligase Midline-1-like isoform X2
XM_029843784.1	XP_029699644.1	E3 ubiquitin-protein ligase Midline-1-like
XM_003961874.3	XP_003961923.2	E3 ubiquitin-protein ligase Midline-1-like

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo28921	XM_011609200.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OTo00917	XM_011609188.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OTo00917	XM_011609195.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OTo00917	XM_011609176.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OTo00917	XM_003961874.2 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OTo42309	XM_003961874.3 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo42309	XM_029843784.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo42309	XM_011609176.2 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo42309	XM_029843807.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo42309	XM_011609188.2 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo42309	XM_011609195.2 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OTo42309	XM_029843803.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

LOC101075355 ( XM_011609200.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607502.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTo28921
Clone ID Related Accession (Same CDS sequence)	XM_011609200.1
Accession Version	XM_011609200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	E3 ubiquitin-protein ligase Midline-1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607502.1
CDS	378..2042
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLVNIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMAPASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTW

Target ORF information:

RefSeq Version	XM_011609200.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609200.1

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_003961874.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003961923.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo00917
Clone ID Related Accession (Same CDS sequence)	XM_003961874.2 , XM_011609188.1 , XM_011609195.1 , XM_011609176.1
Accession Version	XM_003961874.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	E3 ubiquitin-protein ligase Midline-1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003961874.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961923.1
CDS	132..2141
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLVNIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMAPASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_003961874.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961874.2

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_011609188.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607490.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo00917
Clone ID Related Accession (Same CDS sequence)	XM_003961874.2 , XM_011609188.1 , XM_011609195.1 , XM_011609176.1
Accession Version	XM_011609188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	E3 ubiquitin-protein ligase Midline-1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607490.1
CDS	1522..3531
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLVNIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMAPASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_011609188.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609188.1

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_011609195.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607497.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo00917
Clone ID Related Accession (Same CDS sequence)	XM_003961874.2 , XM_011609188.1 , XM_011609195.1 , XM_011609176.1
Accession Version	XM_011609195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	E3 ubiquitin-protein ligase Midline-1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607497.1
CDS	725..2734
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLVNIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMAPASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_011609195.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609195.1

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_011609176.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607478.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo00917
Clone ID Related Accession (Same CDS sequence)	XM_003961874.2 , XM_011609188.1 , XM_011609195.1 , XM_011609176.1
Accession Version	XM_011609176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	E3 ubiquitin-protein ligase Midline-1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070905.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607478.1
CDS	378..2387
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLVNIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMAPASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_011609176.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609176.1

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCGTCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CACCAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CAAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_029843803.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029699663.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OTo42309
Clone ID Related Accession (Same CDS sequence)	XM_029843803.1 , XM_011609188.2 , XM_011609176.2 , XM_029843807.1 , XM_011609195.2 , XM_029843784.1 , XM_003961874.3
Accession Version	XM_029843803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase Midline-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029699663.1
CDS	198..2207
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLINIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMATASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_029843803.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029843803.1

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_011609188.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607490.2 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo42309
Clone ID Related Accession (Same CDS sequence)	XM_029843803.1 , XM_011609188.2 , XM_011609176.2 , XM_029843807.1 , XM_011609195.2 , XM_029843784.1 , XM_003961874.3
Accession Version	XM_011609188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase Midline-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607490.2
CDS	424..2433
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLINIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMATASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_011609188.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609188.2

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_011609176.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607478.2 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTo42309
Clone ID Related Accession (Same CDS sequence)	XM_029843803.1 , XM_011609188.2 , XM_011609176.2 , XM_029843807.1 , XM_011609195.2 , XM_029843784.1 , XM_003961874.3
Accession Version	XM_011609176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase Midline-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607478.2
CDS	303..2312
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLINIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMATASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_011609176.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609176.2

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_029843807.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029699667.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X7, mRNA.

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CloneID	OTo42309
Clone ID Related Accession (Same CDS sequence)	XM_029843803.1 , XM_011609188.2 , XM_011609176.2 , XM_029843807.1 , XM_011609195.2 , XM_029843784.1 , XM_003961874.3
Accession Version	XM_029843807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase Midline-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029699667.1
CDS	952..2961
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLINIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMATASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_029843807.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029843807.1

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_011609195.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011607497.2 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo42309
Clone ID Related Accession (Same CDS sequence)	XM_029843803.1 , XM_011609188.2 , XM_011609176.2 , XM_029843807.1 , XM_011609195.2 , XM_029843784.1 , XM_003961874.3
Accession Version	XM_011609195.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase Midline-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011609195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011607497.2
CDS	279..2288
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLINIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMATASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_011609195.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011609195.2

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_029843784.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029699644.1 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo42309
Clone ID Related Accession (Same CDS sequence)	XM_029843803.1 , XM_011609188.2 , XM_011609176.2 , XM_029843807.1 , XM_011609195.2 , XM_029843784.1 , XM_003961874.3
Accession Version	XM_029843784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase Midline-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029699644.1
CDS	1626..3635
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLINIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMATASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_029843784.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029843784.1

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101075355 ( XM_003961874.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003961923.2 Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo42309
Clone ID Related Accession (Same CDS sequence)	XM_029843803.1 , XM_011609188.2 , XM_011609176.2 , XM_029843807.1 , XM_011609195.2 , XM_029843784.1 , XM_003961874.3
Accession Version	XM_003961874.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase Midline-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003961874.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961923.2
CDS	136..2145
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCTPSEPIQSISAFQCPTCRYVITLNQRGLEGLKRNVTLQNIIDRYQKASQSGPNSPKETRREGAVPDSRAMTSPGDRVPCQFCEQDPPQDAVKTCITCEVSYCDECLKATHPNKKPFTGHRLMEPLLDSHLRGIMCAEHEDEKVNMYCVTDEQLICALCKLVGRHRDHHVAALGDRYDKLKESLESNLNNLIKRTSDLESLMGKLIQTCQHVEVNASRQENKLLEECDLLINIIQQRRQIITTKIKEGKAVRLRKLAQQIAGCKQCIERSSSLITQADQALKEADHTRFLQTAKSICERVSMATASSQVLLPEINLNDTFDTFALDFTREKKMLENLDYLTAPNPPVIREELCTASYDTITVHWTSDDEFTVVSYELQYAIFTCQSNVVSLCNSADSWMIVPNIKQNHYTVHGLQCGTKYIFIVKAINQAGNRSSEPAKLKTNSQPFRLDPKSAHRKLRVSHDNLTVERDETSAKKSHSQDRFTSHSSYGVTGNVYIDSGRHYWEALIGGSTWFAVGVAYKSAPRHEWVGKNSASWVLSRCNNSWVARHNSKEMPVEPPPHLRRLGILLDYDSGSLSFYDAVSSQHLYTFDVAFAQPVCPVFNVWNRCLTILTGLPIPDHLEGTDYNK

Target ORF information:

RefSeq Version	XM_003961874.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase Midline-1-like (LOC101075355), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961874.3

ORF Insert Sequence:

ATGGAAACGC TGGAGTCGGA GCTGACCTGT CCAATCTGTC TGGAGCTATT TGAGGACCCG 
CTGCTCTTGC CCTGCGCTCA CAGCCTTTGT TTCAACTGCG CCCACCGCAT CCTGGTGTCA 
CACTGCACGC CCAGCGAGCC CATCCAATCC ATCAGCGCCT TCCAGTGCCC GACCTGCCGC 
TATGTCATCA CCCTCAACCA GAGGGGCCTA GAGGGACTCA AACGCAACGT TACGCTGCAG 
AACATCATTG ACCGTTACCA GAAGGCATCG CAAAGCGGAC CCAACTCTCC CAAAGAGACG 
CGACGCGAGG GAGCCGTCCC CGACAGCAGA GCCATGACGT CACCCGGCGA CAGGGTGCCG 
TGTCAGTTCT GTGAGCAGGA TCCTCCTCAG GATGCCGTGA AGACCTGCAT CACCTGCGAG 
GTGTCCTACT GCGACGAGTG TCTCAAGGCC ACCCACCCCA ACAAGAAGCC GTTCACGGGT 
CACCGCCTCA TGGAGCCTCT GCTGGACTCC CATCTGCGGG GGATAATGTG CGCGGAGCAC 
GAGGACGAGA AGGTCAACAT GTACTGTGTG ACCGACGAAC AATTGATCTG CGCATTGTGC 
AAGCTGGTTG GTCGACACAG GGACCATCAC GTGGCGGCCC TCGGCGATCG ATACGACAAA 
CTCAAGGAAT CCTTGGAATC TAACCTCAAC AATCTAATCA AGAGGACCAG TGATTTGGAA 
AGTCTGATGG GTAAACTTAT TCAAACCTGC CAGCACGTCG AGGTAAATGC ATCCAGACAG 
GAAAACAAGC TGCTGGAGGA GTGTGACCTG CTCATCAACA TCATACAGCA GCGAAGACAA 
ATAATAACCA CCAAAATAAA AGAAGGAAAG GCCGTGCGGC TGAGGAAGCT CGCCCAGCAG 
ATAGCCGGCT GCAAGCAGTG CATCGAGAGG TCCTCTTCCC TCATCACACA AGCCGACCAG 
GCGCTCAAGG AGGCGGACCA CACTCGTTTC CTCCAAACTG CTAAAAGCAT CTGTGAGAGG 
GTTTCCATGG CAACAGCATC TTCACAAGTG TTGTTACCAG AGATAAACTT GAATGACACA 
TTTGATACTT TTGCTCTGGA TTTCACACGG GAGAAGAAAA TGCTTGAAAA TTTAGATTAC 
CTCACAGCAC CGAATCCTCC AGTAATCCGC GAGGAGCTGT GCACAGCTTC GTACGACACC 
ATCACAGTTC ACTGGACGTC GGACGACGAG TTCACCGTGG TGTCCTATGA ACTTCAGTAC 
GCCATCTTCA CCTGCCAGTC CAACGTCGTC AGTTTGTGCA ACTCTGCCGA CAGCTGGATG 
ATTGTACCAA ACATCAAGCA GAACCACTAC ACTGTGCACG GGCTCCAGTG CGGAACCAAG 
TACATCTTTA TTGTGAAAGC CATAAACCAG GCGGGAAACC GCAGCAGCGA ACCAGCAAAA 
CTCAAAACTA ACAGTCAGCC CTTCAGGTTG GACCCAAAGT CAGCTCACCG AAAGCTGAGG 
GTGTCTCACG ACAACCTGAC GGTGGAGAGG GACGAGACGT CGGCCAAAAA GAGCCACAGT 
CAGGACCGAT TCACGAGCCA CAGCAGCTAC GGCGTGACGG GAAACGTCTA CATCGACAGC 
GGGCGCCATT ACTGGGAAGC TCTGATTGGA GGAAGCACAT GGTTTGCAGT GGGAGTTGCA 
TACAAGTCGG CGCCGAGACA CGAGTGGGTG GGCAAAAACT CGGCCTCCTG GGTGCTGTCC 
AGATGCAACA ACTCGTGGGT GGCGCGCCAC AACAGCAAGG AGATGCCCGT GGAGCCCCCG 
CCACACCTGC GACGCCTGGG AATATTGTTG GACTACGACT CCGGATCTCT GTCTTTCTAC 
GACGCTGTGA GCTCGCAGCA CTTGTACACG TTCGACGTCG CTTTTGCTCA GCCGGTCTGT 
CCTGTGTTTA ACGTGTGGAA CAGATGTCTG ACAATCCTCA CCGGACTCCC CATCCCAGAC 
CACTTAGAGG GGACAGACTA CAACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC101075355 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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