LOC101070901 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101070901
Entrez Gene ID	101070901
Full Name	retinoblastoma-like protein 2
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003967330.2	XP_003967379.1	retinoblastoma-like protein 2
XM_003967330.3	XP_003967379.1	retinoblastoma-like protein 2

Pathways from BioSystems
Gene Ontology

The following LOC101070901 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101070901 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo57909	XM_003967330.3 Latest version!	Takifugu rubripes retinoblastoma-like protein 2 (LOC101070901), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo06286	XM_003967330.2 Latest version!	Takifugu rubripes retinoblastoma-like protein 2 (LOC101070901), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101070901 ( XM_003967330.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003967379.1 Takifugu rubripes retinoblastoma-like protein 2 (LOC101070901), mRNA.

Price & Availability↑

CloneID	OTo57909
Clone ID Related Accession (Same CDS sequence)	XM_003967330.3
Accession Version	XM_003967330.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	retinoblastoma-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003967330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACG AGGACGACAG CTTGCAGATC GCTCGAGCCG GGTTTGAAGA CCTGTGTCGG 
GCTTTAAATA TGGACGAAGA GGCGGCCAAC GAGGCTTGGA AAACCTACGA AAGCATCAGT 
AGAAACTTTA CATTAGAGGG TAGCGAGCTG CACTGGCTGG CCTGTTCTCT GTATGTTGCC 
TGCAGGTCGT CTGTACCAAC GGTGGGAAAA GGCACCTCCG AGGGGAATTA CGTGTCTTTA 
ACCAGAATCC TGCGCTGCTC AGAGATGAGC CTGATAGAGT TCTTTAACAA GATGAAGAAG 
TGGCAGGACA TGGCCAATCT GCCACAGGAG TTTCGTCAAA GCACTAACAA GCTGGAGAGA 
AATTTCACAG TTTCAGCCGT GATCTTTAAG AAGTACGTCC CCATCTTCAA GTCCATCTTC 
AAGGCGCCAT CAGAGGAGCC TCCTCGAGTT CACCGCAGTC GCAAGCAGAG ACGTCATCCC 
TGCACCATAA GTGAAGTCTT CAACTTCTGC TGGGTTTTGT TTGTCCATGC TAAAGGGAAC 
TTCCCTATGA TCAGTGATGA CCTGGTCAAC TCGTATCATC TGCTGTTGTG TGCGCTCGAC 
CTTGTCCTCA CCAATGCTCT GCTTTGCAAT GCCAGGAAGG AGTTGCTCAA CCCAAACTTT 
AAAGGACTGC CGGAGGACTT CAGCAGTAAA GACTACAGGC CGTGTGCAGG GCCGTACTGC 
TTCATGGAGC AGCTGTGTAA GCTGCACGAT GGTCTGGTGC TGGAAGCCAA AGGAGTGAAA 
GAGCATTTCT GGAAACCGTT CATTAAAAAG CTTTTCCACA AGAGGATTCT CAGAGGAAAA 
GAGGACAGTC TGACTGGCTT TCTGGATCCT ATGAACTTTG GAGACAGTTT TCTGTCTCTC 
AGTCGGGTGT ATGAAGAACA CATTCTGGCT ACCGGCAGTC TGGATGAAAG AATCTTCACT 
GGCGAGGGCG CAAGTGAAGA GATTGGAACT CCTGGGCCCT GTCTGTATGA GGGGTTGGAG 
AACCAGAACA GCGCCATCAG CAGACTACAC GTCAGCCTCA CCGCCTCAGC TCTGAAGGTT 
GCCACTCCTC TAACAGGAAG GAAATACATT CAGGAGAATG TTGTGGCATC TCCCATCTCA 
ACTGCCATGA ACAGTGTTGG GCGTCTTCAC ACACTGTTGT CAGGGACCCA GCAGGGGCCA 
AGCGCTAAGC TAGCAGAAAC ATTCAGGGCG TGTGCCAGAG ATCCCAGTGA TGTCATCACT 
AGGCGCCTGC AGGGAATGTT TGAGCTCTTC TCTCAGCATT TTGAGGGCAG TGGAGCTGAG 
AACAGGGGGA TGGGGAAAGA AATGGCAGTG AAGTATTTCC ACCTGGCGGA GGCGCTCTAC 
TACAGGACCC TGGAGTCCAT CATCGAGAGA GAGAAGATGA TTCTGGGAAA TGCTGACTTG 
TCATGTATTC TGGAGCAGGA TGTCTTCCAT CGCTCTCTGC TTGTCTGTTG CCTGGAGATT 
GTCATCTTCT CATACAGACC TCCAGGAGAT TTCCCCAACT TGATCAGTAT CTTCCAGCTG 
CCTGCTTATC ACTTCTACAA GGTGATCGAA GTGTTGGTGC GATCAGAGCA GGGTCTGTTT 
CGGGAGGTGG TGAAGCACCT GAACCAGGTG GAAGAGCAGG TCCTGGAGAG TCTGGCCTGG 
ACCAGCAACT CCCCCCTTTG GGAGACGCTT CGTGGGGCCA AAGACCAAGT CCCAGCCTGC 
AAGCAGGTGA TGCCACCTCA GTATCTGGAG CAGAACAATG ACAGCAGCTC AGGCAGCCTT 
CCCATCACCC CCGTCAACCA GGGACAGAAC CTCAACATCG GCAGCGCTCT CAAAGGCATG 
TCTCCCTCAC CCACCACTCT GCTGGACCGC TACAGTTCTC CACCTGTCGG CCCTTTTCGC 
CGACGGCTGT TTGTCGAGCA GCAGGACAGC CAACCTGGAG CCACCGCCAA AAACGGTGTC 
AGCACCGCCG GCCCGGCACC GGCCACTCTG ACCAAAGTCG ACCAGCCCGG CCTCATCACG 
GCCATCACAG CCAGACAGGG CGTGGTCACT GTGGGAGCCG CCACCATGAC TGCTGAGAAT 
GGGCAGACAC TCACCATCCC TATGCAGGGT ATCGCCAATG AGAGCGGTGG AATTACCCTC 
ATCCCGCTCC AGGTGAGCGT GTCGGGACAG GGCGGAGCTA CGCTCCATAC GTTGCCTGCT 
CAGACCCTGA CCGGCACGGT CGCCGTCCAA TCAGCCGTCA CCAAAGCAGC TACCAAACCC 
ATCAGTCCTC TGAGGAAAGG TTCTCTGTCA CTCTTCTTCA GAAAGCTGTA CCACATGGCC 
AGTGTGCGCC TCAGAGATCT GTGTGCCAAA CTGGACATTT CTGCAGAGTT GAGGAGGAAG 
ATCTGGACGT GTTTTGAATA TTCTCTGGTT CACTGCACCA ACCTGATGAT GGACCGACAC 
CTGGACCAAC TCCTGATGTG TGCAGTTTAC ATCATGTCAA AGGTGACCAA AGAAGACAGA 
TCCTTCCAGA ACATCATGAA GTGCTACCGT ACTCAGCCGC AGGCCGGCAG CAATGTGTAC 
CGGTGTGTGT TAATCTCAGG TAGGAGGAAA CACCAATCAG GCAGCAACGG CACAAAGAAA 
ACTGACTCAA CTGCTGACAT CAGCCAGGAT TCAGCTGATG GAGACAACAG TCCAGTTCCT 
GTTCGCTCCA GCAGCACGTT GCCCACCTCT CAGCCTGGAA GTGCCCCCTC CACCCCCACC 
AGCAACTCCA TCAAGACATT GCCTTCGTCC CTTCCTGGGG GTGAGGTTGA GGAAAAGGAG 
GAGCAAGGTG ACCTGATCCA TTTCTACAAC CAGGTTTATG TTAAGCAGAT GAGGAGCTTT 
GCTCTCAGAT ACTCCGTCAG CTCTCTGGCT ACAGCAGTGG ACTCTCCCCC TCTCTGCCCA 
TACCCCACCC TGCGGACAGG TTCACCTCGT CGGATGCTTC TGTCCAGCAA ACATTCTATC 
TTCATCTCAC CTCACAAGAC AGGCTCCACC CCCTCCGCCT CCCCACGAGA CAAGATTTAT 
TACTACATTT GCAGCAGTCC CCCCAACCGC CTCCAGGAGA TCAACAGCAT GATACGGACT 
GGGGAGTCCC CCACCAAGAA GCGAAGTATT CCTCTGGAGG AGGAGACATC AGCCAAAAGA 
ATTTGTTCTG ATAGCCATTC TGCTCTGTTG CGCCGCCTAC AGGATGTTGC CAATGACCGA 
AGCTCCACCC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003967379.1
CDS	181..3435
Translation	MTDEDDSLQIARAGFEDLCRALNMDEEAANEAWKTYESISRNFTLEGSELHWLACSLYVACRSSVPTVGKGTSEGNYVSLTRILRCSEMSLIEFFNKMKKWQDMANLPQEFRQSTNKLERNFTVSAVIFKKYVPIFKSIFKAPSEEPPRVHRSRKQRRHPCTISEVFNFCWVLFVHAKGNFPMISDDLVNSYHLLLCALDLVLTNALLCNARKELLNPNFKGLPEDFSSKDYRPCAGPYCFMEQLCKLHDGLVLEAKGVKEHFWKPFIKKLFHKRILRGKEDSLTGFLDPMNFGDSFLSLSRVYEEHILATGSLDERIFTGEGASEEIGTPGPCLYEGLENQNSAISRLHVSLTASALKVATPLTGRKYIQENVVASPISTAMNSVGRLHTLLSGTQQGPSAKLAETFRACARDPSDVITRRLQGMFELFSQHFEGSGAENRGMGKEMAVKYFHLAEALYYRTLESIIEREKMILGNADLSCILEQDVFHRSLLVCCLEIVIFSYRPPGDFPNLISIFQLPAYHFYKVIEVLVRSEQGLFREVVKHLNQVEEQVLESLAWTSNSPLWETLRGAKDQVPACKQVMPPQYLEQNNDSSSGSLPITPVNQGQNLNIGSALKGMSPSPTTLLDRYSSPPVGPFRRRLFVEQQDSQPGATAKNGVSTAGPAPATLTKVDQPGLITAITARQGVVTVGAATMTAENGQTLTIPMQGIANESGGITLIPLQVSVSGQGGATLHTLPAQTLTGTVAVQSAVTKAATKPISPLRKGSLSLFFRKLYHMASVRLRDLCAKLDISAELRRKIWTCFEYSLVHCTNLMMDRHLDQLLMCAVYIMSKVTKEDRSFQNIMKCYRTQPQAGSNVYRCVLISGRRKHQSGSNGTKKTDSTADISQDSADGDNSPVPVRSSSTLPTSQPGSAPSTPTSNSIKTLPSSLPGGEVEEKEEQGDLIHFYNQVYVKQMRSFALRYSVSSLATAVDSPPLCPYPTLRTGSPRRMLLSSKHSIFISPHKTGSTPSASPRDKIYYYICSSPPNRLQEINSMIRTGESPTKKRSIPLEEETSAKRICSDSHSALLRRLQDVANDRSSTH

Target ORF information:

RefSeq Version	XM_003967330.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes retinoblastoma-like protein 2 (LOC101070901), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003967330.3

ORF Insert Sequence:

ATGACCGACG AGGACGACAG CTTGCAGATC GCTCGAGCCG GGTTTGAAGA CCTGTGTCGG 
GCTTTAAATA TGGACGAAGA GGCGGCCAAC GAGGCTTGGA AAACCTACGA AAGCATCAGT 
AGAAACTTTA CATTAGAGGG TAGCGAGCTG CACTGGCTGG CCTGTTCTCT GTATGTTGCC 
TGCAGGTCGT CTGTACCAAC GGTGGGAAAA GGCACCTCCG AGGGGAATTA CGTGTCTTTA 
ACCAGAATCC TGCGCTGCTC AGAGATGAGC CTGATAGAGT TCTTTAACAA GATGAAGAAG 
TGGCAGGACA TGGCCAATCT GCCACAGGAG TTTCGTCAAA GCACTAACAA GCTGGAGAGA 
AATTTCACAG TTTCAGCCGT GATCTTTAAG AAGTACGTCC CCATCTTCAA GTCCATCTTC 
AAGGCGCCAT CAGAGGAGCC TCCTCGAGTT CACCGCAGTC GCAAGCAGAG ACGTCATCCC 
TGCACCATAA GTGAAGTCTT CAACTTCTGC TGGGTTTTGT TTGTCCATGC TAAAGGGAAC 
TTCCCTATGA TCAGTGATGA CCTGGTCAAC TCGTATCATC TGCTGTTGTG TGCGCTCGAC 
CTTGTCCTCA CCAATGCTCT GCTTTGCAAT GCCAGGAAGG AGTTGCTCAA CCCAAACTTT 
AAAGGACTGC CGGAGGACTT CAGCAGTAAA GACTACAGGC CGTGTGCAGG GCCGTACTGC 
TTCATGGAGC AGCTGTGTAA GCTGCACGAT GGTCTGGTGC TGGAAGCCAA AGGAGTGAAA 
GAGCATTTCT GGAAACCGTT CATTAAAAAG CTTTTCCACA AGAGGATTCT CAGAGGAAAA 
GAGGACAGTC TGACTGGCTT TCTGGATCCT ATGAACTTTG GAGACAGTTT TCTGTCTCTC 
AGTCGGGTGT ATGAAGAACA CATTCTGGCT ACCGGCAGTC TGGATGAAAG AATCTTCACT 
GGCGAGGGCG CAAGTGAAGA GATTGGAACT CCTGGGCCCT GTCTGTATGA GGGGTTGGAG 
AACCAGAACA GCGCCATCAG CAGACTACAC GTCAGCCTCA CCGCCTCAGC TCTGAAGGTT 
GCCACTCCTC TAACAGGAAG GAAATACATT CAGGAGAATG TTGTGGCATC TCCCATCTCA 
ACTGCCATGA ACAGTGTTGG GCGTCTTCAC ACACTGTTGT CAGGGACCCA GCAGGGGCCA 
AGCGCTAAGC TAGCAGAAAC ATTCAGGGCG TGTGCCAGAG ATCCCAGTGA TGTCATCACT 
AGGCGCCTGC AGGGAATGTT TGAGCTCTTC TCTCAGCATT TTGAGGGCAG TGGAGCTGAG 
AACAGGGGGA TGGGGAAAGA AATGGCAGTG AAGTATTTCC ACCTGGCGGA GGCGCTCTAC 
TACAGGACCC TGGAGTCCAT CATCGAGAGA GAGAAGATGA TTCTGGGAAA TGCTGACTTG 
TCATGTATTC TGGAGCAGGA TGTCTTCCAT CGCTCTCTGC TTGTCTGTTG CCTGGAGATT 
GTCATCTTCT CATACAGACC TCCAGGAGAT TTCCCCAACT TGATCAGTAT CTTCCAGCTG 
CCTGCTTATC ACTTCTACAA GGTGATCGAA GTGTTGGTGC GATCAGAGCA GGGTCTGTTT 
CGGGAGGTGG TGAAGCACCT GAACCAGGTG GAAGAGCAGG TCCTGGAGAG TCTGGCCTGG 
ACCAGCAACT CCCCCCTTTG GGAGACGCTT CGTGGGGCCA AAGACCAAGT CCCAGCCTGC 
AAGCAGGTGA TGCCACCTCA GTATCTGGAG CAGAACAATG ACAGCAGCTC AGGCAGCCTT 
CCCATCACCC CCGTCAACCA GGGACAGAAC CTCAACATCG GCAGCGCTCT CAAAGGCATG 
TCTCCCTCAC CCACCACTCT GCTGGACCGC TACAGTTCTC CACCTGTCGG CCCTTTTCGC 
CGACGGCTGT TTGTCGAGCA GCAGGACAGC CAACCTGGAG CCACCGCCAA AAACGGTGTC 
AGCACCGCCG GCCCGGCACC GGCCACTCTG ACCAAAGTCG ACCAGCCCGG CCTCATCACG 
GCCATCACAG CCAGACAGGG CGTGGTCACT GTGGGAGCCG CCACCATGAC TGCTGAGAAT 
GGGCAGACAC TCACCATCCC TATGCAGGGT ATCGCCAATG AGAGCGGTGG AATTACCCTC 
ATCCCGCTCC AGGTGAGCGT GTCGGGACAG GGCGGAGCTA CGCTCCATAC GTTGCCTGCT 
CAGACCCTGA CCGGCACGGT CGCCGTCCAA TCAGCCGTCA CCAAAGCAGC TACCAAACCC 
ATCAGTCCTC TGAGGAAAGG TTCTCTGTCA CTCTTCTTCA GAAAGCTGTA CCACATGGCC 
AGTGTGCGCC TCAGAGATCT GTGTGCCAAA CTGGACATTT CTGCAGAGTT GAGGAGGAAG 
ATCTGGACGT GTTTTGAATA TTCTCTGGTT CACTGCACCA ACCTGATGAT GGACCGACAC 
CTGGACCAAC TCCTGATGTG TGCAGTTTAC ATCATGTCAA AGGTGACCAA AGAAGACAGA 
TCCTTCCAGA ACATCATGAA GTGCTACCGT ACTCAGCCGC AGGCCGGCAG CAATGTGTAC 
CGGTGTGTGT TAATCTCAGG TAGGAGGAAA CACCAATCAG GCAGCAACGG CACAAAGAAA 
ACTGACTCAA CTGCTGACAT CAGCCAGGAT TCAGCTGATG GAGACAACAG TCCAGTTCCT 
GTTCGCTCCA GCAGCACGTT GCCCACCTCT CAGCCTGGAA GTGCCCCCTC CACCCCCACC 
AGCAACTCCA TCAAGACATT GCCTTCGTCC CTTCCTGGGG GTGAGGTTGA GGAAAAGGAG 
GAGCAAGGTG ACCTGATCCA TTTCTACAAC CAGGTTTATG TTAAGCAGAT GAGGAGCTTT 
GCTCTCAGAT ACTCCGTCAG CTCTCTGGCT ACAGCAGTGG ACTCTCCCCC TCTCTGCCCA 
TACCCCACCC TGCGGACAGG TTCACCTCGT CGGATGCTTC TGTCCAGCAA ACATTCTATC 
TTCATCTCAC CTCACAAGAC AGGCTCCACC CCCTCCGCCT CCCCACGAGA CAAGATTTAT 
TACTACATTT GCAGCAGTCC CCCCAACCGC CTCCAGGAGA TCAACAGCAT GATACGGACT 
GGGGAGTCCC CCACCAAGAA GCGAAGTATT CCTCTGGAGG AGGAGACATC AGCCAAAAGA 
ATTTGTTCTG ATAGCCATTC TGCTCTGTTG CGCCGCCTAC AGGATGTTGC CAATGACCGA 
AGCTCCACCC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101070901 ( XM_003967330.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003967379.1 Takifugu rubripes retinoblastoma-like protein 2 (LOC101070901), mRNA.

Price & Availability↑

CloneID	OTo06286
Clone ID Related Accession (Same CDS sequence)	XM_003967330.2
Accession Version	XM_003967330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	retinoblastoma-like protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071014.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003967330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACG AGGACGACAG CTTGCAGATC GCTCGAGCCG GGTTTGAAGA CCTGTGTCGG 
GCTTTAAATA TGGACGAAGA GGCGGCCAAC GAGGCTTGGA AAACCTACGA AAGCATCAGT 
AGAAACTTTA CATTAGAGGG TAGCGAGCTG CACTGGCTGG CCTGTTCTCT GTATGTTGCC 
TGCAGGTCGT CTGTGCCAAC GGTGGGAAAA GGCACCTCCG AGGGGAATTA CGTGTCTTTA 
ACCAGAATCC TGCGCTGCTC AGAGATGAGC CTGATAGAGT TCTTTAACAA GATGAAGAAG 
TGGCAGGACA TGGCCAATCT GCCACAGGAG TTTCGTCAAA GCACTAACAA GCTGGAGAGA 
AATTTCACAG TTTCAGCCGT GATCTTTAAG AAGTACGTCC CCATCTTCAA GTCCATCTTC 
AAGGCGCCAT CAGAGGAGCC TCCTCGAGTT CACCGCAGTC GCAAGCAGAG ACGTCATCCC 
TGCACCATAA GTGAAGTCTT CAACTTCTGC TGGGTTTTGT TTGTCCATGC TAAAGGGAAC 
TTCCCTATGA TCAGTGATGA CCTGGTCAAC TCGTATCATC TGCTGTTGTG TGCGCTCGAC 
CTTGTCCTCA CCAATGCTCT GCTTTGCAAT GCCAGGAAGG AGTTGCTCAA CCCAAACTTT 
AAAGGACTGC CGGAGGACTT CAGCAGTAAA GACTACAGGC CGTGTGCAGG GCCGTACTGC 
TTCATGGAGC AGCTGTGTAA GCTGCACGAT GGTCTGGTGC TGGAAGCCAA AGGAGTGAAA 
GAGCATTTCT GGAAACCGTT CATTAAAAAG CTTTTCCACA AGAGGATTCT CAGAGGAAAA 
GAGGACAGTC TGACTGGCTT TCTGGATCCT ATGAACTTTG GAGACAGTTT TCTGTCTCTC 
AGTCGGGTGT ATGAAGAACA CATTCTGGCT ACCGGCAGTC TGGATGAAAG AATCTTCACT 
GGCGAGGGCG CAAGTGAAGA GATTGGAACT CCTGGGCCCT GTCTGTATGA GGGGTTGGAG 
AACCAGAACA GCGCCATCAG CAGACTACAC GTCAGCCTCA CCGCCTCAGC TCTGAAGGTT 
GCCACTCCTC TAACAGGAAG GAAATACATT CAGGAGAATG TTGTGGCATC TCCCATCTCA 
ACTGCCATGA ACAGTGTTGG GCGTCTTCAC ACACTGTTGT CAGGGACCCA GCAGGGGCCA 
AGCGCTAAGC TAGCAGAAAC ATTCAGGGCG TGTGCCAGAG ATCCCAGTGA TGTCATCACT 
AGGCGCCTGC AGGGAATGTT TGAGCTCTTC TCTCAGCATT TTGAGGGCAG TGGAGCTGAG 
AACAGGGGGA TGGGGAAAGA AATGGCAGTG AAGTATTTCC ACCTGGCGGA GGCGCTCTAC 
TACAGGACCC TGGAGTCCAT CATCGAGAGA GAGAAGATGA TTCTGGGAAA TGCTGACTTG 
TCATGTATTC TGGAGCAGGA TGTCTTCCAT CGCTCTCTGC TTGTCTGTTG CCTGGAGATT 
GTCATCTTCT CATACAGACC TCCAGGAGAT TTCCCCAACT TGATCAGTAT CTTCCAGCTG 
CCTGCTTATC ACTTCTACAA GGTGATCGAA GTGTTGGTGC GATCAGAGCA GGGTCTGTTT 
CGGGAGGTGG TGAAGCACCT GAACCAGGTG GAAGAGCAGG TCCTGGAGAG TCTGGCCTGG 
ACCAGCAACT CCCCCCTTTG GGAGACGCTT CGTGGGGCCA AAGACCAAGT CCCAGCCTGC 
AAGCAGGTGA TGCCACCTCA GTATCTGGAG CAGAACAATG ACAGCAGCTC AGGCAGCCTT 
CCCATCACCC CCGTCAACCA GGGACAGAAC CTCAACATCG GCAGCGCTCT CAAAGGCATG 
TCTCCCTCAC CCACCACTCT GCTGGACCGC TACAGTTCTC CACCTGTCGG CCCTTTTCGC 
CGACGGCTGT TTGTCGAGCA GCAGGACAGC CAACCTGGAG CCACCGCCAA AAACGGTGTC 
AGCACCGCCG GCCCGGCACC GGCCACTCTG ACCAAAGTCG ACCAGCCCGG CCTCATCACG 
GCCATCACAG CCAGACAGGG CGTGGTCACT GTGGGAGCCG CCACCATGAC TGCTGAGAAT 
GGGCAGACAC TCACCATCCC TATGCAGGGT ATCGCCAATG AGAGCGGTGG AATTACCCTC 
ATCCCGCTCC AGGTGAGCGT GTCGGGACAG GGCGGAGCTA CGCTCCATAC GTTGCCTGCT 
CAGACCCTGA CCGGCACGGT CGCCGTCCAA TCAGCCGTCA CCAAAGCAGC TACCAAACCC 
ATCAGTCCTC TGAGGAAAGG TTCTCTGTCA CTCTTCTTCA GAAAGCTGTA CCACATGGCC 
AGTGTGCGCC TCAGAGATCT GTGTGCCAAA CTGGACATTT CTGCAGAGTT GAGAAGGAAG 
ATCTGGACGT GTTTTGAATA TTCTCTGGTT CACTGCACCA ACCTGATGAT GGACCGACAC 
CTGGACCAAC TCCTGATGTG TGCAGTTTAC ATCATGTCAA AGGTGACCAA AGAAGACAGA 
TCCTTCCAGA ACATCATGAA GTGCTACCGT ACTCAGCCGC AGGCCGGCAG CAATGTGTAC 
CGGTGTGTGT TAATCTCAGG TAGGAGGAAA CACCAATCAG GCAGCAACGG CACAAAGAAA 
ACTGACTCAA CTGCTGACAT CAGCCAGGAT TCAGCTGATG GAGACAACAG TCCAGTTCCT 
GTTCGCTCCA GCAGCACGTT GCCCACCTCT CAGCCTGGAA GTGCCCCCTC CACCCCCACC 
AGCAACTCCA TCAAGACATT GCCTTCGTCC CTTCCTGGGG GTGAGGTTGA GGAAAAGGAG 
GAGCAAGGTG ACCTGATCCA TTTCTACAAC CAGGTTTATG TTAAGCAGAT GAGGAGCTTT 
GCTCTCAGAT ACTCCGTCAG CTCTCTGGCT ACAGCAGTGG ACTCTCCCCC TCTCTGCCCA 
TACCCCACCC TGCGGACAGG TTCACCTCGT CGGATGCTTC TGTCCAGCAA ACATTCTATC 
TTCATCTCAC CTCACAAGAC AGGCTCCACC CCCTCCGCCT CCCCACGAGA CAAGATTTAT 
TACTACATTT GCAGCAGTCC CCCCAACCGC CTCCAGGAGA TCAACAGCAT GATACGGACT 
GGGGAGTCCC CCACCAAGAA GCGAAGTATT CCTCTGGAGG AGGAGACATC AGCCAAAAGA 
ATTTGTTCTG ATAGCCATTC TGCTCTGTTG CGCCGCCTAC AGGATGTTGC CAATGACCGA 
AGCTCCACCC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003967379.1
CDS	158..3412
Translation	MTDEDDSLQIARAGFEDLCRALNMDEEAANEAWKTYESISRNFTLEGSELHWLACSLYVACRSSVPTVGKGTSEGNYVSLTRILRCSEMSLIEFFNKMKKWQDMANLPQEFRQSTNKLERNFTVSAVIFKKYVPIFKSIFKAPSEEPPRVHRSRKQRRHPCTISEVFNFCWVLFVHAKGNFPMISDDLVNSYHLLLCALDLVLTNALLCNARKELLNPNFKGLPEDFSSKDYRPCAGPYCFMEQLCKLHDGLVLEAKGVKEHFWKPFIKKLFHKRILRGKEDSLTGFLDPMNFGDSFLSLSRVYEEHILATGSLDERIFTGEGASEEIGTPGPCLYEGLENQNSAISRLHVSLTASALKVATPLTGRKYIQENVVASPISTAMNSVGRLHTLLSGTQQGPSAKLAETFRACARDPSDVITRRLQGMFELFSQHFEGSGAENRGMGKEMAVKYFHLAEALYYRTLESIIEREKMILGNADLSCILEQDVFHRSLLVCCLEIVIFSYRPPGDFPNLISIFQLPAYHFYKVIEVLVRSEQGLFREVVKHLNQVEEQVLESLAWTSNSPLWETLRGAKDQVPACKQVMPPQYLEQNNDSSSGSLPITPVNQGQNLNIGSALKGMSPSPTTLLDRYSSPPVGPFRRRLFVEQQDSQPGATAKNGVSTAGPAPATLTKVDQPGLITAITARQGVVTVGAATMTAENGQTLTIPMQGIANESGGITLIPLQVSVSGQGGATLHTLPAQTLTGTVAVQSAVTKAATKPISPLRKGSLSLFFRKLYHMASVRLRDLCAKLDISAELRRKIWTCFEYSLVHCTNLMMDRHLDQLLMCAVYIMSKVTKEDRSFQNIMKCYRTQPQAGSNVYRCVLISGRRKHQSGSNGTKKTDSTADISQDSADGDNSPVPVRSSSTLPTSQPGSAPSTPTSNSIKTLPSSLPGGEVEEKEEQGDLIHFYNQVYVKQMRSFALRYSVSSLATAVDSPPLCPYPTLRTGSPRRMLLSSKHSIFISPHKTGSTPSASPRDKIYYYICSSPPNRLQEINSMIRTGESPTKKRSIPLEEETSAKRICSDSHSALLRRLQDVANDRSSTH

Target ORF information:

RefSeq Version	XM_003967330.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes retinoblastoma-like protein 2 (LOC101070901), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003967330.2

ORF Insert Sequence:

ATGACCGACG AGGACGACAG CTTGCAGATC GCTCGAGCCG GGTTTGAAGA CCTGTGTCGG 
GCTTTAAATA TGGACGAAGA GGCGGCCAAC GAGGCTTGGA AAACCTACGA AAGCATCAGT 
AGAAACTTTA CATTAGAGGG TAGCGAGCTG CACTGGCTGG CCTGTTCTCT GTATGTTGCC 
TGCAGGTCGT CTGTGCCAAC GGTGGGAAAA GGCACCTCCG AGGGGAATTA CGTGTCTTTA 
ACCAGAATCC TGCGCTGCTC AGAGATGAGC CTGATAGAGT TCTTTAACAA GATGAAGAAG 
TGGCAGGACA TGGCCAATCT GCCACAGGAG TTTCGTCAAA GCACTAACAA GCTGGAGAGA 
AATTTCACAG TTTCAGCCGT GATCTTTAAG AAGTACGTCC CCATCTTCAA GTCCATCTTC 
AAGGCGCCAT CAGAGGAGCC TCCTCGAGTT CACCGCAGTC GCAAGCAGAG ACGTCATCCC 
TGCACCATAA GTGAAGTCTT CAACTTCTGC TGGGTTTTGT TTGTCCATGC TAAAGGGAAC 
TTCCCTATGA TCAGTGATGA CCTGGTCAAC TCGTATCATC TGCTGTTGTG TGCGCTCGAC 
CTTGTCCTCA CCAATGCTCT GCTTTGCAAT GCCAGGAAGG AGTTGCTCAA CCCAAACTTT 
AAAGGACTGC CGGAGGACTT CAGCAGTAAA GACTACAGGC CGTGTGCAGG GCCGTACTGC 
TTCATGGAGC AGCTGTGTAA GCTGCACGAT GGTCTGGTGC TGGAAGCCAA AGGAGTGAAA 
GAGCATTTCT GGAAACCGTT CATTAAAAAG CTTTTCCACA AGAGGATTCT CAGAGGAAAA 
GAGGACAGTC TGACTGGCTT TCTGGATCCT ATGAACTTTG GAGACAGTTT TCTGTCTCTC 
AGTCGGGTGT ATGAAGAACA CATTCTGGCT ACCGGCAGTC TGGATGAAAG AATCTTCACT 
GGCGAGGGCG CAAGTGAAGA GATTGGAACT CCTGGGCCCT GTCTGTATGA GGGGTTGGAG 
AACCAGAACA GCGCCATCAG CAGACTACAC GTCAGCCTCA CCGCCTCAGC TCTGAAGGTT 
GCCACTCCTC TAACAGGAAG GAAATACATT CAGGAGAATG TTGTGGCATC TCCCATCTCA 
ACTGCCATGA ACAGTGTTGG GCGTCTTCAC ACACTGTTGT CAGGGACCCA GCAGGGGCCA 
AGCGCTAAGC TAGCAGAAAC ATTCAGGGCG TGTGCCAGAG ATCCCAGTGA TGTCATCACT 
AGGCGCCTGC AGGGAATGTT TGAGCTCTTC TCTCAGCATT TTGAGGGCAG TGGAGCTGAG 
AACAGGGGGA TGGGGAAAGA AATGGCAGTG AAGTATTTCC ACCTGGCGGA GGCGCTCTAC 
TACAGGACCC TGGAGTCCAT CATCGAGAGA GAGAAGATGA TTCTGGGAAA TGCTGACTTG 
TCATGTATTC TGGAGCAGGA TGTCTTCCAT CGCTCTCTGC TTGTCTGTTG CCTGGAGATT 
GTCATCTTCT CATACAGACC TCCAGGAGAT TTCCCCAACT TGATCAGTAT CTTCCAGCTG 
CCTGCTTATC ACTTCTACAA GGTGATCGAA GTGTTGGTGC GATCAGAGCA GGGTCTGTTT 
CGGGAGGTGG TGAAGCACCT GAACCAGGTG GAAGAGCAGG TCCTGGAGAG TCTGGCCTGG 
ACCAGCAACT CCCCCCTTTG GGAGACGCTT CGTGGGGCCA AAGACCAAGT CCCAGCCTGC 
AAGCAGGTGA TGCCACCTCA GTATCTGGAG CAGAACAATG ACAGCAGCTC AGGCAGCCTT 
CCCATCACCC CCGTCAACCA GGGACAGAAC CTCAACATCG GCAGCGCTCT CAAAGGCATG 
TCTCCCTCAC CCACCACTCT GCTGGACCGC TACAGTTCTC CACCTGTCGG CCCTTTTCGC 
CGACGGCTGT TTGTCGAGCA GCAGGACAGC CAACCTGGAG CCACCGCCAA AAACGGTGTC 
AGCACCGCCG GCCCGGCACC GGCCACTCTG ACCAAAGTCG ACCAGCCCGG CCTCATCACG 
GCCATCACAG CCAGACAGGG CGTGGTCACT GTGGGAGCCG CCACCATGAC TGCTGAGAAT 
GGGCAGACAC TCACCATCCC TATGCAGGGT ATCGCCAATG AGAGCGGTGG AATTACCCTC 
ATCCCGCTCC AGGTGAGCGT GTCGGGACAG GGCGGAGCTA CGCTCCATAC GTTGCCTGCT 
CAGACCCTGA CCGGCACGGT CGCCGTCCAA TCAGCCGTCA CCAAAGCAGC TACCAAACCC 
ATCAGTCCTC TGAGGAAAGG TTCTCTGTCA CTCTTCTTCA GAAAGCTGTA CCACATGGCC 
AGTGTGCGCC TCAGAGATCT GTGTGCCAAA CTGGACATTT CTGCAGAGTT GAGAAGGAAG 
ATCTGGACGT GTTTTGAATA TTCTCTGGTT CACTGCACCA ACCTGATGAT GGACCGACAC 
CTGGACCAAC TCCTGATGTG TGCAGTTTAC ATCATGTCAA AGGTGACCAA AGAAGACAGA 
TCCTTCCAGA ACATCATGAA GTGCTACCGT ACTCAGCCGC AGGCCGGCAG CAATGTGTAC 
CGGTGTGTGT TAATCTCAGG TAGGAGGAAA CACCAATCAG GCAGCAACGG CACAAAGAAA 
ACTGACTCAA CTGCTGACAT CAGCCAGGAT TCAGCTGATG GAGACAACAG TCCAGTTCCT 
GTTCGCTCCA GCAGCACGTT GCCCACCTCT CAGCCTGGAA GTGCCCCCTC CACCCCCACC 
AGCAACTCCA TCAAGACATT GCCTTCGTCC CTTCCTGGGG GTGAGGTTGA GGAAAAGGAG 
GAGCAAGGTG ACCTGATCCA TTTCTACAAC CAGGTTTATG TTAAGCAGAT GAGGAGCTTT 
GCTCTCAGAT ACTCCGTCAG CTCTCTGGCT ACAGCAGTGG ACTCTCCCCC TCTCTGCCCA 
TACCCCACCC TGCGGACAGG TTCACCTCGT CGGATGCTTC TGTCCAGCAA ACATTCTATC 
TTCATCTCAC CTCACAAGAC AGGCTCCACC CCCTCCGCCT CCCCACGAGA CAAGATTTAT 
TACTACATTT GCAGCAGTCC CCCCAACCGC CTCCAGGAGA TCAACAGCAT GATACGGACT 
GGGGAGTCCC CCACCAAGAA GCGAAGTATT CCTCTGGAGG AGGAGACATC AGCCAAAAGA 
ATTTGTTCTG ATAGCCATTC TGCTCTGTTG CGCCGCCTAC AGGATGTTGC CAATGACCGA 
AGCTCCACCC ACTAA