emg1 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	emg1
Entrez Gene ID	101066424
Full Name	EMG1, N1-specific pseudouridine methyltransferase
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003966283.3	XP_003966332.1	ribosomal RNA small subunit methyltransferase NEP1
XM_003966283.2	XP_003966332.1	ribosomal RNA small subunit methyltransferase NEP1

Pathways from BioSystems
Gene Ontology

The following emg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the emg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo05262	XM_003966283.3 Latest version!	Takifugu rubripes EMG1 N1-specific pseudouridine methyltransferase (LOC101066424), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OTo05262	XM_003966283.2	Takifugu rubripes EMG1 N1-specific pseudouridine methyltransferase (emg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

emg1 ( XM_003966283.3 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003966332.1 Takifugu rubripes EMG1 N1-specific pseudouridine methyltransferase (LOC101066424), mRNA.

Price & Availability↑

CloneID	OTo05262
Clone ID Related Accession (Same CDS sequence)	XM_003966283.3 , XM_003966283.2
Accession Version	XM_003966283.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	ribosomal RNA small subunit methyltransferase NEP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021821647.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003966283.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATT CAAGCGAGAA GAAGCGTGGT CTTGAACATT TGGATGAATA TGAACCAAAA 
CCTGCGAAAC ATCTTCGCAG TCTGCACGAT CGCATGGCAG AGAGACGACT GGTTGTTATT 
CTGGAGGGAG CATCGTTAGA AACAGTAAAG GTCGGGAAAA CTTTTGAACT GTTAAACTGT 
GACCAGCACA AAAATATAAT CGTCAAGAAT GGAAGGAATC CAGGCAACAT TAGACCAGAT 
ATCACCCATC AGTCTCTGCT CATGTTGATG GACAGTCCAC TGAACAGGGC AGGACTGTTG 
CAGGTCTATG TTCACACTGA GAAAAATGCC TTAATAGAGA TCAACCCACA GACCCGCATT 
CCAAGAACCT TCACCCGCTT CTGTGGTCTT ATGGTTCAGC TGCTCCACAA GCTGAGTGTC 
AGAGCTGCTG ATGGTCCTCA GAAGCTTCTG AGGATGATTA AAAACCCAGT GTCTGACCAC 
CTTCCTCCGG GCTGCCCACG CATTGGCACC TCCTTCTCCT CAGGAGAGGC TGTTTGCCCC 
CGTACTTTAG TGCCAGAGGG TCCCGCTGCA GTGGTGATCG GAGCGTTCGC ACATGGGGCG 
GTGAACGTGG ACTACACAGA GAAGACTGTG TCCATCAGTA ATTACCCCCT CTCTGCAGCA 
CTGACCTGTG CAAAGATTTG TTCTGCTTTT GAAGAAGTCT GGGGGGTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003966332.1
CDS	52..765
Translation	MADSSEKKRGLEHLDEYEPKPAKHLRSLHDRMAERRLVVILEGASLETVKVGKTFELLNCDQHKNIIVKNGRNPGNIRPDITHQSLLMLMDSPLNRAGLLQVYVHTEKNALIEINPQTRIPRTFTRFCGLMVQLLHKLSVRAADGPQKLLRMIKNPVSDHLPPGCPRIGTSFSSGEAVCPRTLVPEGPAAVVIGAFAHGAVNVDYTEKTVSISNYPLSAALTCAKICSAFEEVWGVL

Target ORF information:

RefSeq Version	XM_003966283.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes EMG1 N1-specific pseudouridine methyltransferase (LOC101066424), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003966283.3

ORF Insert Sequence:

ATGGCGGATT CAAGCGAGAA GAAGCGTGGT CTTGAACATT TGGATGAATA TGAACCAAAA 
CCTGCGAAAC ATCTTCGCAG TCTGCACGAT CGCATGGCAG AGAGACGACT GGTTGTTATT 
CTGGAGGGAG CATCGTTAGA AACAGTAAAG GTCGGGAAAA CTTTTGAACT GTTAAACTGT 
GACCAGCACA AAAATATAAT CGTCAAGAAT GGAAGGAATC CAGGCAACAT TAGACCAGAT 
ATCACCCATC AGTCTCTGCT CATGTTGATG GACAGTCCAC TGAACAGGGC AGGACTGTTG 
CAGGTCTATG TTCACACTGA GAAAAATGCC TTAATAGAGA TCAACCCACA GACCCGCATT 
CCAAGAACCT TCACCCGCTT CTGTGGTCTT ATGGTTCAGC TGCTCCACAA GCTGAGTGTC 
AGAGCTGCTG ATGGTCCTCA GAAGCTTCTG AGGATGATTA AAAACCCAGT GTCTGACCAC 
CTTCCTCCGG GCTGCCCACG CATTGGCACC TCCTTCTCCT CAGGAGAGGC TGTTTGCCCC 
CGTACTTTAG TGCCAGAGGG TCCCGCTGCA GTGGTGATCG GAGCGTTCGC ACATGGGGCG 
GTGAACGTGG ACTACACAGA GAAGACTGTG TCCATCAGTA ATTACCCCCT CTCTGCAGCA 
CTGACCTGTG CAAAGATTTG TTCTGCTTTT GAAGAAGTCT GGGGGGTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

emg1 ( XM_003966283.2 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_003966332.1 Takifugu rubripes EMG1 N1-specific pseudouridine methyltransferase (emg1), mRNA.

Price & Availability↑

CloneID	OTo05262
Clone ID Related Accession (Same CDS sequence)	XM_003966283.3 , XM_003966283.2
Accession Version	XM_003966283.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	ribosomal RNA small subunit methyltransferase NEP1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070991.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003966283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATT CAAGCGAGAA GAAGCGTGGT CTTGAACATT TGGATGAATA TGAACCAAAA 
CCTGCGAAAC ATCTTCGCAG TCTGCACGAT CGCATGGCAG AGAGACGACT GGTTGTTATT 
CTGGAGGGAG CATCGTTAGA AACAGTAAAG GTCGGGAAAA CTTTTGAACT GTTAAACTGT 
GACCAGCACA AAAATATAAT CGTCAAGAAT GGAAGGAATC CAGGCAACAT TAGACCAGAT 
ATCACCCATC AGTCTCTGCT CATGTTGATG GACAGTCCAC TGAACAGGGC AGGACTGTTG 
CAGGTCTATG TTCACACTGA GAAAAATGCC TTAATAGAGA TCAACCCACA GACCCGCATT 
CCAAGAACCT TCACCCGCTT CTGTGGTCTT ATGGTTCAGC TGCTCCACAA GCTGAGTGTC 
AGAGCTGCTG ATGGTCCTCA GAAGCTTCTG AGGATGATTA AAAACCCAGT GTCTGACCAC 
CTTCCTCCGG GCTGCCCACG CATTGGCACC TCCTTCTCCT CAGGAGAGGC TGTTTGCCCC 
CGTACTTTAG TGCCAGAGGG TCCCGCTGCA GTGGTGATCG GAGCGTTCGC ACATGGGGCG 
GTGAACGTGG ACTACACAGA GAAGACTGTG TCCATCAGTA ATTACCCCCT CTCTGCAGCA 
CTGACCTGTG CAAAGATTTG TTCTGCTTTT GAAGAAGTCT GGGGGGTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003966332.1
CDS	71..784
Translation	MADSSEKKRGLEHLDEYEPKPAKHLRSLHDRMAERRLVVILEGASLETVKVGKTFELLNCDQHKNIIVKNGRNPGNIRPDITHQSLLMLMDSPLNRAGLLQVYVHTEKNALIEINPQTRIPRTFTRFCGLMVQLLHKLSVRAADGPQKLLRMIKNPVSDHLPPGCPRIGTSFSSGEAVCPRTLVPEGPAAVVIGAFAHGAVNVDYTEKTVSISNYPLSAALTCAKICSAFEEVWGVL

Target ORF information:

RefSeq Version	XM_003966283.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes EMG1 N1-specific pseudouridine methyltransferase (emg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003966283.2

ORF Insert Sequence:

ATGGCGGATT CAAGCGAGAA GAAGCGTGGT CTTGAACATT TGGATGAATA TGAACCAAAA 
CCTGCGAAAC ATCTTCGCAG TCTGCACGAT CGCATGGCAG AGAGACGACT GGTTGTTATT 
CTGGAGGGAG CATCGTTAGA AACAGTAAAG GTCGGGAAAA CTTTTGAACT GTTAAACTGT 
GACCAGCACA AAAATATAAT CGTCAAGAAT GGAAGGAATC CAGGCAACAT TAGACCAGAT 
ATCACCCATC AGTCTCTGCT CATGTTGATG GACAGTCCAC TGAACAGGGC AGGACTGTTG 
CAGGTCTATG TTCACACTGA GAAAAATGCC TTAATAGAGA TCAACCCACA GACCCGCATT 
CCAAGAACCT TCACCCGCTT CTGTGGTCTT ATGGTTCAGC TGCTCCACAA GCTGAGTGTC 
AGAGCTGCTG ATGGTCCTCA GAAGCTTCTG AGGATGATTA AAAACCCAGT GTCTGACCAC 
CTTCCTCCGG GCTGCCCACG CATTGGCACC TCCTTCTCCT CAGGAGAGGC TGTTTGCCCC 
CGTACTTTAG TGCCAGAGGG TCCCGCTGCA GTGGTGATCG GAGCGTTCGC ACATGGGGCG 
GTGAACGTGG ACTACACAGA GAAGACTGTG TCCATCAGTA ATTACCCCCT CTCTGCAGCA 
CTGACCTGTG CAAAGATTTG TTCTGCTTTT GAAGAAGTCT GGGGGGTTTT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.