smg5 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	smg5
Entrez Gene ID	101065130
Full Name	SMG5, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003965642.2	XP_003965691.1	protein SMG5
XM_003965642.3	XP_003965691.1	protein SMG5

Pathways from BioSystems
Gene Ontology

The following smg5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smg5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo69613	XM_003965642.3 Latest version!	Takifugu rubripes SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo04627	XM_003965642.2 Latest version!	Takifugu rubripes SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smg5 ( XM_003965642.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003965691.1 Takifugu rubripes SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.

Price & Availability↑

CloneID	OTo69613
Clone ID Related Accession (Same CDS sequence)	XM_003965642.3
Accession Version	XM_003965642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	protein SMG5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042291.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003965642.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAC CGGGGCAAGA CAACGAACCA GAGGCAAAAG TCCTTCTCAT CAAGCGGCTT 
TACAGGGCTG TGGTGGAGTC CGTGCACAAG CTGGACGTTA TCACCAGCGG CAAGGTGTCC 
CACAGAGAGG TCTTCAAACC GGAGAACATC AGCCTCCGGA ACAAGTTGCG GGAGCTTTGT 
GTGAAGCTGA TGTTCCTCCA CCCTGTCGAT TATGGCCGTA AGGCCGAGGA GCTTCTGTGG 
AGGAAGGTTT ATTATGACAT CATTCAGGTT ATCAAGACAA ATAAGAAGCA CATCCACAGC 
CGCAGTTCCC TGGAGTGTGC TTATCGTACA CATCTGATCG CCGGTGTAGG TTTTTACCAA 
CATCTTCTGC TGTACATCCA GTCACATTAC CAGCTGGAGC TGCAGGACTG TATAGACTGG 
ACCCATGTAA CAGACCCATT GGTCGGCCAG AAGAAGCCAG TGTCCGCCAC CACCAAGGAG 
ATGGAGTGGG CACAGATGGC CTGTCATCGC TGTCTGGTCT ACCTCGGAGA TTTAGCCCGT 
TATCAAAATG AACTTGCTGG AGTAGATGCT GAGCAACTAG CAGAAAGATT TTACCACCAG 
GCTTTGTCTG TCATGCCACA CATTGGAATG CCTTTTAACC AACTGGGCAC ACTGGCTGGG 
AGCAAGTTCT ACAATGTTGA AGCAACATAC TACTACCTGC GCTGTATCCA ATCAGATGTT 
CCCTTTGATG GAGCCTATGG AAACCTAAAG CGCCTGTTTG ACAAAGCAGC CAAGATGTAC 
CACCAAGTGA AGAAGCAGGA GATAAAGAAA CATTCTCCAT CTCAACAAAG ATCAAAAGAT 
ATAAAGCGCC TCCTGGTGAG CTTCATGTAC CTCCAAAGCC TGCTGCATCC CAAGAACAGT 
CAGGTGGAGG TGGAGCTGAC TACACTCTGC CAGTCAGTGC TGGAAGACTT CAACCTGGTG 
CTGTTTTACT TGCCGGCACA GACGCTGGGC GGAGCTCGCC ACTCCCACAG TGAGGAGGAG 
GAGCTGCAGT CGCAGCAGCA ACAGCAGCAT CATGTTGACA GCTGCTCTAC TGTTTTACCT 
GATACTCTGA TCTTCAAGAT GGTGGTCATA TGTCTGATGG TGGTGCACAG CCTGAAGAGA 
GGAGGGTCAA AGCAGTACAG TGCATCCATA GCTTTTACTC TGGCACTGTT CTCTCATCTG 
GTGAACCATG TGAACATTCG GCTGCAAGCT GAACTGGAAG AAGCAGACAT ACAGGTTCCT 
CCCCTCCACA CAGATACCAC AGATGACCCT GAGATCCGGG ATTTGTCAAC TCCAACAAAT 
AAACCTGAGG CAAAGTCTCA GCAAAACGGC TCCCTGGAAC TAGATGAGGA GGAGGAGGAA 
AAGGATGAAG ATCTTGGCGA ATGTGTGGAT ACTGGGAAGC ATGAAAATGC AAATGAACAC 
TGCAGAATAG ATGAAGAAAA GCAGAGACAG AAGAAAAAGT ACACCCGGCT GTGTAGGTTA 
CGTCGGCGGC GCTGCAACCG AAAGGATGTC AGGGGTGAGG CAGAGAGTGA CTTGAGTGAA 
GGCTTTGAGA GTGAGGACGA TGAGGAGGAT GAAGAGGAGA TAGGGGGCAC CACTGATTCA 
ACAGGCAGTA CCATGGCGGC AGTACCAGAT AACCCTCCAG CTGTTAGAAA GGAAGTCAAG 
AAGGAGCAAC CGCGTGACGA GGCTAGCTGG GGTAGCGGCT CAGAGGAAGA CGAGGGGGGA 
ACAGCGTTCG ATATAGAGAC TGACTCGGAC ATGAACAGCC AGGAGTCGCG TTCAGATCTT 
GAAGATATGG AGGACACAGA AAAGCCAGAG AACAACGAGG GTCCGGTGCC TGTACCCCGG 
GAAGAAGAAG GGGATGATTA TCAACCTAAG GAAAAAGGAA GTGACACGAA TGGGCCGCTC 
ACACACAGTG ACTCCAGCAT CAGCAGTAAC CTCCAAGCCA TGTCCTCGCA GCTTTTCCAG 
GCAAAACGAT GCTTTCGCTT AGCACCAACC TTCAGTAATA TGCTGCTGAG GCCTCAAGCC 
TCATCTTCCT CTCCAAATCC CAACCTCACC GAAGATTCTG CTCCACCCTC CCAAGAGACC 
CCTCCTCCTC AGGACTCTGC CTGCGATATG AATCCTGGTA CTGCCAATGG AACTAATGAA 
AACGATTTGG ACTCTGACTC CGAATGCAGC CAGAACAGTT GTCAGTCAGT GCGCAGTGAG 
AAAACACTCT TGGAGAAACT AGAGATACTG ACTAATCAGG GCTTGATCCA AGTAGTGAAG 
ATTTTTGTTG ATTGGCTGCG AGCAAACACT GACATTATCC TGATGTGTGC CCAGAGTTCT 
CAGAGCCTGT GGAATCGTCT GTCTGTGTTG CTCAACCTCC TCCCAGATGG CAGAAAGTTA 
CTGGAATCCG ATCTGGGTCT GCACACAGAG GTGCAGGAGC TCGTGAATGA GTGTGAGCAG 
CCTGGTTTGA CCCAGAGTCT TCCACTGCCG GAGGATCTGG CTCTGAGGCA CCTGCCCGCT 
CTCAGCCTCG CCCACCGACG TCTTGATTTC ACTCATCGCT ACCCTGCTCT CTCGTCCCTG 
CAGGAGTGTG TCGTACGGGT CTGTTGCATT CGGAGTTTTG GGCACTTCTT AACAAATCTC 
CACGGCAACG TGCTGCACTT CAACCCAGAG GTCGGGATCT TCACCAGCAT CACTCAGTCT 
GAACAGGACA ACCTGGTGCA GCAGGCCAAG GCCCAGTTCA AAATGGCTGA AGAGGAGGCC 
CGTCGAAATC GCTTGATGAG GGATATGGCC CAGCTTCGAC TGCAGTTGGA GGTGTCACAG 
CTAGAGGGCA GCCTTCAGCA GCCAAAGGCG CAGTCGTCCA TGTCTCCGTA CCTGGTGCCA 
GACGCAGCTG CACTCTGTCA GCATTTGAAC CTCATCCGAC AGCTGGCAAA GAGCGGTTGC 
TTCATCATCA TCATCCCACG GACAGTGATT GATGGACTTG ACCACTTAAA GAAGGAAAAT 
GCTGGCGCCA GAGACGGCAT TCGTTTCCTA GAATCGGAGT TTCGCAAAGG CAACAGGTAC 
ATTCGCTGCC AAAAAGAGTC CGGTCGAAGT TTTGAAAGAG ACAAACTGAA ACGTCAGGAC 
ATGGAAGCCT GGCATTTATG GAAGATGGTG GACAGCTGTC GTCAGCTTAT AACGGTCTCC 
CAGAGCAATG GAGATGAGGA CACGGCTGGC ATGGTGACGA TTCTCACTGG TCACCAAGTG 
GAGCAACTGT GCTCCCAATC CACAGCAATG AAGTCAGCGG TGCAGGCGGT GTGCTCGGCA 
GGCATGGAGC TGAAAAACAT CAAGGAGTTC TATCGACAGT GGAAGGAGAT CGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003965691.1
CDS	365..3721
Translation	MSGPGQDNEPEAKVLLIKRLYRAVVESVHKLDVITSGKVSHREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYDIIQVIKTNKKHIHSRSSLECAYRTHLIAGVGFYQHLLLYIQSHYQLELQDCIDWTHVTDPLVGQKKPVSATTKEMEWAQMACHRCLVYLGDLARYQNELAGVDAEQLAERFYHQALSVMPHIGMPFNQLGTLAGSKFYNVEATYYYLRCIQSDVPFDGAYGNLKRLFDKAAKMYHQVKKQEIKKHSPSQQRSKDIKRLLVSFMYLQSLLHPKNSQVEVELTTLCQSVLEDFNLVLFYLPAQTLGGARHSHSEEEELQSQQQQQHHVDSCSTVLPDTLIFKMVVICLMVVHSLKRGGSKQYSASIAFTLALFSHLVNHVNIRLQAELEEADIQVPPLHTDTTDDPEIRDLSTPTNKPEAKSQQNGSLELDEEEEEKDEDLGECVDTGKHENANEHCRIDEEKQRQKKKYTRLCRLRRRRCNRKDVRGEAESDLSEGFESEDDEEDEEEIGGTTDSTGSTMAAVPDNPPAVRKEVKKEQPRDEASWGSGSEEDEGGTAFDIETDSDMNSQESRSDLEDMEDTEKPENNEGPVPVPREEEGDDYQPKEKGSDTNGPLTHSDSSISSNLQAMSSQLFQAKRCFRLAPTFSNMLLRPQASSSSPNPNLTEDSAPPSQETPPPQDSACDMNPGTANGTNENDLDSDSECSQNSCQSVRSEKTLLEKLEILTNQGLIQVVKIFVDWLRANTDIILMCAQSSQSLWNRLSVLLNLLPDGRKLLESDLGLHTEVQELVNECEQPGLTQSLPLPEDLALRHLPALSLAHRRLDFTHRYPALSSLQECVVRVCCIRSFGHFLTNLHGNVLHFNPEVGIFTSITQSEQDNLVQQAKAQFKMAEEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSSMSPYLVPDAAALCQHLNLIRQLAKSGCFIIIIPRTVIDGLDHLKKENAGARDGIRFLESEFRKGNRYIRCQKESGRSFERDKLKRQDMEAWHLWKMVDSCRQLITVSQSNGDEDTAGMVTILTGHQVEQLCSQSTAMKSAVQAVCSAGMELKNIKEFYRQWKEIG

Target ORF information:

RefSeq Version	XM_003965642.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003965642.3

ORF Insert Sequence:

ATGAGTGGAC CGGGGCAAGA CAACGAACCA GAGGCAAAAG TCCTTCTCAT CAAGCGGCTT 
TACAGGGCTG TGGTGGAGTC CGTGCACAAG CTGGACGTTA TCACCAGCGG CAAGGTGTCC 
CACAGAGAGG TCTTCAAACC GGAGAACATC AGCCTCCGGA ACAAGTTGCG GGAGCTTTGT 
GTGAAGCTGA TGTTCCTCCA CCCTGTCGAT TATGGCCGTA AGGCCGAGGA GCTTCTGTGG 
AGGAAGGTTT ATTATGACAT CATTCAGGTT ATCAAGACAA ATAAGAAGCA CATCCACAGC 
CGCAGTTCCC TGGAGTGTGC TTATCGTACA CATCTGATCG CCGGTGTAGG TTTTTACCAA 
CATCTTCTGC TGTACATCCA GTCACATTAC CAGCTGGAGC TGCAGGACTG TATAGACTGG 
ACCCATGTAA CAGACCCATT GGTCGGCCAG AAGAAGCCAG TGTCCGCCAC CACCAAGGAG 
ATGGAGTGGG CACAGATGGC CTGTCATCGC TGTCTGGTCT ACCTCGGAGA TTTAGCCCGT 
TATCAAAATG AACTTGCTGG AGTAGATGCT GAGCAACTAG CAGAAAGATT TTACCACCAG 
GCTTTGTCTG TCATGCCACA CATTGGAATG CCTTTTAACC AACTGGGCAC ACTGGCTGGG 
AGCAAGTTCT ACAATGTTGA AGCAACATAC TACTACCTGC GCTGTATCCA ATCAGATGTT 
CCCTTTGATG GAGCCTATGG AAACCTAAAG CGCCTGTTTG ACAAAGCAGC CAAGATGTAC 
CACCAAGTGA AGAAGCAGGA GATAAAGAAA CATTCTCCAT CTCAACAAAG ATCAAAAGAT 
ATAAAGCGCC TCCTGGTGAG CTTCATGTAC CTCCAAAGCC TGCTGCATCC CAAGAACAGT 
CAGGTGGAGG TGGAGCTGAC TACACTCTGC CAGTCAGTGC TGGAAGACTT CAACCTGGTG 
CTGTTTTACT TGCCGGCACA GACGCTGGGC GGAGCTCGCC ACTCCCACAG TGAGGAGGAG 
GAGCTGCAGT CGCAGCAGCA ACAGCAGCAT CATGTTGACA GCTGCTCTAC TGTTTTACCT 
GATACTCTGA TCTTCAAGAT GGTGGTCATA TGTCTGATGG TGGTGCACAG CCTGAAGAGA 
GGAGGGTCAA AGCAGTACAG TGCATCCATA GCTTTTACTC TGGCACTGTT CTCTCATCTG 
GTGAACCATG TGAACATTCG GCTGCAAGCT GAACTGGAAG AAGCAGACAT ACAGGTTCCT 
CCCCTCCACA CAGATACCAC AGATGACCCT GAGATCCGGG ATTTGTCAAC TCCAACAAAT 
AAACCTGAGG CAAAGTCTCA GCAAAACGGC TCCCTGGAAC TAGATGAGGA GGAGGAGGAA 
AAGGATGAAG ATCTTGGCGA ATGTGTGGAT ACTGGGAAGC ATGAAAATGC AAATGAACAC 
TGCAGAATAG ATGAAGAAAA GCAGAGACAG AAGAAAAAGT ACACCCGGCT GTGTAGGTTA 
CGTCGGCGGC GCTGCAACCG AAAGGATGTC AGGGGTGAGG CAGAGAGTGA CTTGAGTGAA 
GGCTTTGAGA GTGAGGACGA TGAGGAGGAT GAAGAGGAGA TAGGGGGCAC CACTGATTCA 
ACAGGCAGTA CCATGGCGGC AGTACCAGAT AACCCTCCAG CTGTTAGAAA GGAAGTCAAG 
AAGGAGCAAC CGCGTGACGA GGCTAGCTGG GGTAGCGGCT CAGAGGAAGA CGAGGGGGGA 
ACAGCGTTCG ATATAGAGAC TGACTCGGAC ATGAACAGCC AGGAGTCGCG TTCAGATCTT 
GAAGATATGG AGGACACAGA AAAGCCAGAG AACAACGAGG GTCCGGTGCC TGTACCCCGG 
GAAGAAGAAG GGGATGATTA TCAACCTAAG GAAAAAGGAA GTGACACGAA TGGGCCGCTC 
ACACACAGTG ACTCCAGCAT CAGCAGTAAC CTCCAAGCCA TGTCCTCGCA GCTTTTCCAG 
GCAAAACGAT GCTTTCGCTT AGCACCAACC TTCAGTAATA TGCTGCTGAG GCCTCAAGCC 
TCATCTTCCT CTCCAAATCC CAACCTCACC GAAGATTCTG CTCCACCCTC CCAAGAGACC 
CCTCCTCCTC AGGACTCTGC CTGCGATATG AATCCTGGTA CTGCCAATGG AACTAATGAA 
AACGATTTGG ACTCTGACTC CGAATGCAGC CAGAACAGTT GTCAGTCAGT GCGCAGTGAG 
AAAACACTCT TGGAGAAACT AGAGATACTG ACTAATCAGG GCTTGATCCA AGTAGTGAAG 
ATTTTTGTTG ATTGGCTGCG AGCAAACACT GACATTATCC TGATGTGTGC CCAGAGTTCT 
CAGAGCCTGT GGAATCGTCT GTCTGTGTTG CTCAACCTCC TCCCAGATGG CAGAAAGTTA 
CTGGAATCCG ATCTGGGTCT GCACACAGAG GTGCAGGAGC TCGTGAATGA GTGTGAGCAG 
CCTGGTTTGA CCCAGAGTCT TCCACTGCCG GAGGATCTGG CTCTGAGGCA CCTGCCCGCT 
CTCAGCCTCG CCCACCGACG TCTTGATTTC ACTCATCGCT ACCCTGCTCT CTCGTCCCTG 
CAGGAGTGTG TCGTACGGGT CTGTTGCATT CGGAGTTTTG GGCACTTCTT AACAAATCTC 
CACGGCAACG TGCTGCACTT CAACCCAGAG GTCGGGATCT TCACCAGCAT CACTCAGTCT 
GAACAGGACA ACCTGGTGCA GCAGGCCAAG GCCCAGTTCA AAATGGCTGA AGAGGAGGCC 
CGTCGAAATC GCTTGATGAG GGATATGGCC CAGCTTCGAC TGCAGTTGGA GGTGTCACAG 
CTAGAGGGCA GCCTTCAGCA GCCAAAGGCG CAGTCGTCCA TGTCTCCGTA CCTGGTGCCA 
GACGCAGCTG CACTCTGTCA GCATTTGAAC CTCATCCGAC AGCTGGCAAA GAGCGGTTGC 
TTCATCATCA TCATCCCACG GACAGTGATT GATGGACTTG ACCACTTAAA GAAGGAAAAT 
GCTGGCGCCA GAGACGGCAT TCGTTTCCTA GAATCGGAGT TTCGCAAAGG CAACAGGTAC 
ATTCGCTGCC AAAAAGAGTC CGGTCGAAGT TTTGAAAGAG ACAAACTGAA ACGTCAGGAC 
ATGGAAGCCT GGCATTTATG GAAGATGGTG GACAGCTGTC GTCAGCTTAT AACGGTCTCC 
CAGAGCAATG GAGATGAGGA CACGGCTGGC ATGGTGACGA TTCTCACTGG TCACCAAGTG 
GAGCAACTGT GCTCCCAATC CACAGCAATG AAGTCAGCGG TGCAGGCGGT GTGCTCGGCA 
GGCATGGAGC TGAAAAACAT CAAGGAGTTC TATCGACAGT GGAAGGAGAT CGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smg5 ( XM_003965642.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003965691.1 Takifugu rubripes SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.

Price & Availability↑

CloneID	OTo04627
Clone ID Related Accession (Same CDS sequence)	XM_003965642.2
Accession Version	XM_003965642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	protein SMG5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070976.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003965642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAC CGGGGCAAGA CAACGAACCA GAGGCAAAAG TCCTTCTCAT CAAGCGGCTT 
TACAGGGCTG TGGTGGAGTC CGTGCACAAG CTGGACGTTA TCACCAGCGG CAAGGTGTCC 
CACAGAGAGG TCTTCAAACC GGAGAACATC AGCCTCCGGA ACAAGTTGCG GGAGCTTTGT 
GTGAAGCTGA TGTTCCTCCA CCCTGTCGAT TATGGCCGTA AGGCCGAGGA GCTTCTGTGG 
AGGAAGGTTT ATTATGACAT CATTCAGGTT ATCAAGACAA ATAAGAAGCA CATCCACAGC 
CGCAGTTCCC TGGAGTGTGC TTATCGTACA CATCTGATCG CCGGTGTAGG TTTTTACCAA 
CATCTTCTGC TGTACATCCA GTCACATTAC CAGCTGGAGC TGCAGGACTG TATAGACTGG 
ACCCATGTAA CAGACCCATT GGTCGGCCAG AAGAAGCCAG TGTCCGCCAC CACCAAGGAG 
ATGGAGTGGG CACAGATGGC CTGTCATCGC TGTCTGGTCT ACCTCGGAGA TTTAGCCCGT 
TATCAAAATG AACTTGCTGG AGTAGATGCT GAGCAACTAG CAGAAAGATT TTACCACCAG 
GCTTTGTCTG TCATGCCACA CATTGGAATG CCTTTTAACC AACTGGGCAC ACTGGCTGGG 
AGCAAGTTCT ACAATGTTGA AGCAACATAC TACTACCTGC GCTGTATCCA ATCAGATGTT 
CCCTTTGATG GAGCCTATGG AAACCTAAAG CGCCTGTTTG ACAAAGCAGC CAAGATGTAC 
CACCAAGTGA AGAAGCAGGA GATAAAGAAA CATTCTCCAT CTCAACAAAG ATCAAAAGAT 
ATAAAGCGCC TCCTGGTGAG CTTCATGTAC CTCCAAAGCC TGCTGCATCC CAAGAACAGT 
CAGGTGGAGG TGGAGCTGAC TACACTCTGC CAGTCAGTGC TGGAAGACTT CAACCTGGTG 
CTGTTTTACT TGCCGGCACA GACGCTGGGC GGAGCTCGCC ACTCCCACAG TGAGGAGGAG 
GAGCTGCAGT CGCAGCAGCA ACAGCAGCAT CATGTTGACA GCTGCTCTAC TGTTTTACCT 
GATACTCTGA TCTTCAAGAT GGTGGTCATA TGTCTGATGG TGGTGCACAG CCTGAAGAGA 
GGAGGGTCAA AGCAGTACAG TGCATCCATA GCTTTTACTC TGGCACTGTT CTCTCATCTG 
GTGAACCATG TGAACATTCG GCTGCAAGCT GAACTGGAAG AAGCAGACAT ACAGGTTCCT 
CCCCTCCACA CAGATACCAC AGATGACCCT GAGATCCGGG ATTTGTCAAC ACCAACAAAT 
AAACCTGAGG CAAAGTCTCA GCAAAACGGC TCCCTGGAAC TAGATGAGGA GGAGGAGGAA 
AAGGATGAAG ATCTTGGCGA ATGTGTGGAT ACTGGGAAGC ATGAAAATGC AAATGAACAC 
TGCAGAATAG ATGAAGAAAA GCAGAGACAG AAGAAAAAGT ACACCCGGCT GTGTAGGTTA 
CGTCGGCGGC GCTGCAACCG AAAGGATGTC AGGGGTGAGG CAGAGAGTGA CTTGAGTGAA 
GGCTTTGAGA GTGAGGACGA TGAGGAGGAT GAAGAGGAGA TAGGGGGCAC CACTGATTCA 
ACAGGCAGTA CCATGGCGGC AGTACCAGAT AACCCTCCAG CTGTTAGAAA GGAAGTCAAG 
AAGGAGCAAC CGCGTGACGA GGCTAGCTGG GGTAGCGGCT CAGAGGAAGA CGAGGGGGGA 
ACAGCGTTCG ATATAGAGAC TGACTCGGAC ATGAACAGCC AGGAGTCGCG TTCAGATCTT 
GAAGATATGG AGGACACAGA AAAGCCAGAG AACAACGAGG GTCCGGTGCC TGTACCCCGG 
GAAGAAGAAG GGGATGATTA TCAACCTAAG GAAAAAGGAA GTGACACGAA TGGGCCGCTC 
ACACACAGTG ACTCCAGCAT CAGCAGTAAC CTCCAAGCCA TGTCCTCGCA GCTTTTCCAG 
GCAAAACGAT GCTTTCGCTT AGCACCAACC TTCAGTAATA TGCTGCTGAG GCCTCAAGCC 
TCATCTTCCT CTCCAAATCC CAACCTCACC GAAGATTCTG CTCCACCCTC CCAAGAGACC 
CCTCCTCCTC AGGACTCTGC CTGCGATATG AATCCTGGTA CTGCCAATGG AACTAATGAA 
AACGATTTGG ACTCTGACTC CGAATGCAGC CAGAACAGTT GTCAGTCAGT GCGCAGTGAG 
AAAACACTCT TGGAGAAACT AGAGATACTG ACTAATCAGG GCTTGATCCA AGTAGTGAAG 
ATTTTTGTTG ATTGGCTGCG AGCAAACACT GACATTATCC TGATGTGTGC CCAGAGTTCT 
CAGAGCCTGT GGAATCGTCT GTCTGTGTTG CTCAACCTCC TCCCAGATGG CAGAAAGTTA 
CTGGAATCCG ATCTGGGTCT GCACACAGAG GTGCAGGAGC TCGTGAATGA GTGCGAGCAG 
CCTGGTTTGA CCCAGAGTCT TCCACTGCCG GAGGATCTGG CTCTGAGGCA CCTGCCCGCT 
CTCAGCCTCG CCCACCGACG TCTTGATTTC ACTCATCGCT ACCCTGCTCT CTCGTCCCTG 
CAGGAGTGTG TCGTACGGGT CTGTTGCATT CGGAGTTTTG GGCACTTCTT AACAAATCTC 
CACGGCAACG TGCTGCACTT CAACCCAGAG GTCGGGATCT TCACCAGCAT CACTCAGTCT 
GAACAGGACA ACCTGGTGCA GCAGGCCAAG GCCCAGTTCA AAATGGCTGA AGAGGAGGCC 
CGTCGAAATC GCTTGATGAG GGATATGGCC CAGCTTCGAC TGCAGTTGGA GGTGTCACAG 
CTAGAGGGCA GCCTTCAGCA GCCAAAGGCG CAGTCGTCCA TGTCTCCGTA CCTGGTGCCA 
GACGCAGCTG CACTCTGTCA GCATTTGAAC CTCATCCGAC AGCTGGCAAA GAGCGGTTGC 
TTCATCATCA TCATCCCACG GACAGTGATT GATGGACTTG ACCACTTAAA GAAGGAAAAT 
GCTGGCGCCA GAGACGGCAT TCGTTTCCTA GAATCGGAGT TTCGCAAAGG CAACAGGTAC 
ATTCGCTGCC AAAAAGAGTC CGGTCGAAGT TTTGAAAGAG ACAAACTGAA ACGTCAGGAC 
ATGGAAGCCT GGCATTTATG GAAGATGGTG GACAGCTGTC GTCAGCTTAT AACGGTCTCC 
CAGAGCAATG GAGATGAGGA CACGGCTGGC ATGGTGACGA TTCTCACTGG TCACCAAGTG 
GAGCAACTGT GCTCCCAATC CACAGCAATG AAGTCAGCGG TGCAGGCGGT GTGCTCGGCA 
GGCATGGAGC TGAAAAACAT CAAGGAGTTC TATCGACAGT GGAAGGAGAT CGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003965691.1
CDS	355..3711
Translation	MSGPGQDNEPEAKVLLIKRLYRAVVESVHKLDVITSGKVSHREVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYDIIQVIKTNKKHIHSRSSLECAYRTHLIAGVGFYQHLLLYIQSHYQLELQDCIDWTHVTDPLVGQKKPVSATTKEMEWAQMACHRCLVYLGDLARYQNELAGVDAEQLAERFYHQALSVMPHIGMPFNQLGTLAGSKFYNVEATYYYLRCIQSDVPFDGAYGNLKRLFDKAAKMYHQVKKQEIKKHSPSQQRSKDIKRLLVSFMYLQSLLHPKNSQVEVELTTLCQSVLEDFNLVLFYLPAQTLGGARHSHSEEEELQSQQQQQHHVDSCSTVLPDTLIFKMVVICLMVVHSLKRGGSKQYSASIAFTLALFSHLVNHVNIRLQAELEEADIQVPPLHTDTTDDPEIRDLSTPTNKPEAKSQQNGSLELDEEEEEKDEDLGECVDTGKHENANEHCRIDEEKQRQKKKYTRLCRLRRRRCNRKDVRGEAESDLSEGFESEDDEEDEEEIGGTTDSTGSTMAAVPDNPPAVRKEVKKEQPRDEASWGSGSEEDEGGTAFDIETDSDMNSQESRSDLEDMEDTEKPENNEGPVPVPREEEGDDYQPKEKGSDTNGPLTHSDSSISSNLQAMSSQLFQAKRCFRLAPTFSNMLLRPQASSSSPNPNLTEDSAPPSQETPPPQDSACDMNPGTANGTNENDLDSDSECSQNSCQSVRSEKTLLEKLEILTNQGLIQVVKIFVDWLRANTDIILMCAQSSQSLWNRLSVLLNLLPDGRKLLESDLGLHTEVQELVNECEQPGLTQSLPLPEDLALRHLPALSLAHRRLDFTHRYPALSSLQECVVRVCCIRSFGHFLTNLHGNVLHFNPEVGIFTSITQSEQDNLVQQAKAQFKMAEEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSSMSPYLVPDAAALCQHLNLIRQLAKSGCFIIIIPRTVIDGLDHLKKENAGARDGIRFLESEFRKGNRYIRCQKESGRSFERDKLKRQDMEAWHLWKMVDSCRQLITVSQSNGDEDTAGMVTILTGHQVEQLCSQSTAMKSAVQAVCSAGMELKNIKEFYRQWKEIG

Target ORF information:

RefSeq Version	XM_003965642.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes SMG5 nonsense mediated mRNA decay factor (smg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003965642.2

ORF Insert Sequence:

ATGAGTGGAC CGGGGCAAGA CAACGAACCA GAGGCAAAAG TCCTTCTCAT CAAGCGGCTT 
TACAGGGCTG TGGTGGAGTC CGTGCACAAG CTGGACGTTA TCACCAGCGG CAAGGTGTCC 
CACAGAGAGG TCTTCAAACC GGAGAACATC AGCCTCCGGA ACAAGTTGCG GGAGCTTTGT 
GTGAAGCTGA TGTTCCTCCA CCCTGTCGAT TATGGCCGTA AGGCCGAGGA GCTTCTGTGG 
AGGAAGGTTT ATTATGACAT CATTCAGGTT ATCAAGACAA ATAAGAAGCA CATCCACAGC 
CGCAGTTCCC TGGAGTGTGC TTATCGTACA CATCTGATCG CCGGTGTAGG TTTTTACCAA 
CATCTTCTGC TGTACATCCA GTCACATTAC CAGCTGGAGC TGCAGGACTG TATAGACTGG 
ACCCATGTAA CAGACCCATT GGTCGGCCAG AAGAAGCCAG TGTCCGCCAC CACCAAGGAG 
ATGGAGTGGG CACAGATGGC CTGTCATCGC TGTCTGGTCT ACCTCGGAGA TTTAGCCCGT 
TATCAAAATG AACTTGCTGG AGTAGATGCT GAGCAACTAG CAGAAAGATT TTACCACCAG 
GCTTTGTCTG TCATGCCACA CATTGGAATG CCTTTTAACC AACTGGGCAC ACTGGCTGGG 
AGCAAGTTCT ACAATGTTGA AGCAACATAC TACTACCTGC GCTGTATCCA ATCAGATGTT 
CCCTTTGATG GAGCCTATGG AAACCTAAAG CGCCTGTTTG ACAAAGCAGC CAAGATGTAC 
CACCAAGTGA AGAAGCAGGA GATAAAGAAA CATTCTCCAT CTCAACAAAG ATCAAAAGAT 
ATAAAGCGCC TCCTGGTGAG CTTCATGTAC CTCCAAAGCC TGCTGCATCC CAAGAACAGT 
CAGGTGGAGG TGGAGCTGAC TACACTCTGC CAGTCAGTGC TGGAAGACTT CAACCTGGTG 
CTGTTTTACT TGCCGGCACA GACGCTGGGC GGAGCTCGCC ACTCCCACAG TGAGGAGGAG 
GAGCTGCAGT CGCAGCAGCA ACAGCAGCAT CATGTTGACA GCTGCTCTAC TGTTTTACCT 
GATACTCTGA TCTTCAAGAT GGTGGTCATA TGTCTGATGG TGGTGCACAG CCTGAAGAGA 
GGAGGGTCAA AGCAGTACAG TGCATCCATA GCTTTTACTC TGGCACTGTT CTCTCATCTG 
GTGAACCATG TGAACATTCG GCTGCAAGCT GAACTGGAAG AAGCAGACAT ACAGGTTCCT 
CCCCTCCACA CAGATACCAC AGATGACCCT GAGATCCGGG ATTTGTCAAC ACCAACAAAT 
AAACCTGAGG CAAAGTCTCA GCAAAACGGC TCCCTGGAAC TAGATGAGGA GGAGGAGGAA 
AAGGATGAAG ATCTTGGCGA ATGTGTGGAT ACTGGGAAGC ATGAAAATGC AAATGAACAC 
TGCAGAATAG ATGAAGAAAA GCAGAGACAG AAGAAAAAGT ACACCCGGCT GTGTAGGTTA 
CGTCGGCGGC GCTGCAACCG AAAGGATGTC AGGGGTGAGG CAGAGAGTGA CTTGAGTGAA 
GGCTTTGAGA GTGAGGACGA TGAGGAGGAT GAAGAGGAGA TAGGGGGCAC CACTGATTCA 
ACAGGCAGTA CCATGGCGGC AGTACCAGAT AACCCTCCAG CTGTTAGAAA GGAAGTCAAG 
AAGGAGCAAC CGCGTGACGA GGCTAGCTGG GGTAGCGGCT CAGAGGAAGA CGAGGGGGGA 
ACAGCGTTCG ATATAGAGAC TGACTCGGAC ATGAACAGCC AGGAGTCGCG TTCAGATCTT 
GAAGATATGG AGGACACAGA AAAGCCAGAG AACAACGAGG GTCCGGTGCC TGTACCCCGG 
GAAGAAGAAG GGGATGATTA TCAACCTAAG GAAAAAGGAA GTGACACGAA TGGGCCGCTC 
ACACACAGTG ACTCCAGCAT CAGCAGTAAC CTCCAAGCCA TGTCCTCGCA GCTTTTCCAG 
GCAAAACGAT GCTTTCGCTT AGCACCAACC TTCAGTAATA TGCTGCTGAG GCCTCAAGCC 
TCATCTTCCT CTCCAAATCC CAACCTCACC GAAGATTCTG CTCCACCCTC CCAAGAGACC 
CCTCCTCCTC AGGACTCTGC CTGCGATATG AATCCTGGTA CTGCCAATGG AACTAATGAA 
AACGATTTGG ACTCTGACTC CGAATGCAGC CAGAACAGTT GTCAGTCAGT GCGCAGTGAG 
AAAACACTCT TGGAGAAACT AGAGATACTG ACTAATCAGG GCTTGATCCA AGTAGTGAAG 
ATTTTTGTTG ATTGGCTGCG AGCAAACACT GACATTATCC TGATGTGTGC CCAGAGTTCT 
CAGAGCCTGT GGAATCGTCT GTCTGTGTTG CTCAACCTCC TCCCAGATGG CAGAAAGTTA 
CTGGAATCCG ATCTGGGTCT GCACACAGAG GTGCAGGAGC TCGTGAATGA GTGCGAGCAG 
CCTGGTTTGA CCCAGAGTCT TCCACTGCCG GAGGATCTGG CTCTGAGGCA CCTGCCCGCT 
CTCAGCCTCG CCCACCGACG TCTTGATTTC ACTCATCGCT ACCCTGCTCT CTCGTCCCTG 
CAGGAGTGTG TCGTACGGGT CTGTTGCATT CGGAGTTTTG GGCACTTCTT AACAAATCTC 
CACGGCAACG TGCTGCACTT CAACCCAGAG GTCGGGATCT TCACCAGCAT CACTCAGTCT 
GAACAGGACA ACCTGGTGCA GCAGGCCAAG GCCCAGTTCA AAATGGCTGA AGAGGAGGCC 
CGTCGAAATC GCTTGATGAG GGATATGGCC CAGCTTCGAC TGCAGTTGGA GGTGTCACAG 
CTAGAGGGCA GCCTTCAGCA GCCAAAGGCG CAGTCGTCCA TGTCTCCGTA CCTGGTGCCA 
GACGCAGCTG CACTCTGTCA GCATTTGAAC CTCATCCGAC AGCTGGCAAA GAGCGGTTGC 
TTCATCATCA TCATCCCACG GACAGTGATT GATGGACTTG ACCACTTAAA GAAGGAAAAT 
GCTGGCGCCA GAGACGGCAT TCGTTTCCTA GAATCGGAGT TTCGCAAAGG CAACAGGTAC 
ATTCGCTGCC AAAAAGAGTC CGGTCGAAGT TTTGAAAGAG ACAAACTGAA ACGTCAGGAC 
ATGGAAGCCT GGCATTTATG GAAGATGGTG GACAGCTGTC GTCAGCTTAT AACGGTCTCC 
CAGAGCAATG GAGATGAGGA CACGGCTGGC ATGGTGACGA TTCTCACTGG TCACCAAGTG 
GAGCAACTGT GCTCCCAATC CACAGCAATG AAGTCAGCGG TGCAGGCGGT GTGCTCGGCA 
GGCATGGAGC TGAAAAACAT CAAGGAGTTC TATCGACAGT GGAAGGAGAT CGGCTGA