LOC101063711 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101063711
Entrez Gene ID	101063711
Full Name	potassium voltage-gated channel subfamily H member 4-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003961253.1	XP_003961302.1	potassium voltage-gated channel subfamily H member 4-like
XM_003961253.2	XP_003961302.2	potassium voltage-gated channel subfamily H member 4-like

Pathways from BioSystems
Gene Ontology

The following LOC101063711 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101063711 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo42952	XM_003961253.2 Latest version!	Takifugu rubripes potassium voltage-gated channel subfamily H member 4-like (LOC101063711), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo00303	XM_003961253.1 Latest version!	Takifugu rubripes potassium voltage-gated channel subfamily H member 4-like (LOC101063711), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101063711 ( XM_003961253.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003961302.2 Takifugu rubripes potassium voltage-gated channel subfamily H member 4-like (LOC101063711), mRNA.

Price & Availability↑

CloneID	OTo42952
Clone ID Related Accession (Same CDS sequence)	XM_003961253.2
Accession Version	XM_003961253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	potassium voltage-gated channel subfamily H member 4-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003961253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGA TGAAGGGCCT CCTGGCCCCA CAGAACACCT TTCTGGACGC CATCGCGACA 
CGCTTTGATG GCACCCACAG TAACTTCCTG TTGGGTAACG CCCAGGGCCA CCGCGGCTAC 
CCTATTGTCT ACTGCTCCGA TGGCTTCTGT GAACTGACGG GCTTCACGAG AACCGAGGTG 
ATGCAGAAGA ACTGCGGCTG CCACTTCCTT TTCGGGGCTG ACACCAGCGA GCATGTGGCG 
CAGCAGATGT TGAAGGCTCT GGAGGGTCGC GAAGAGTACC AGGCCGAGGT CTACTTCTAC 
AAGAAAAATG GTGTGGCCTT CTGGTGTCTG CTAGACATTG TGCCCATAAA GAATGAGAAA 
GGCGAAATGG TCCTCTTCCT CTTTTCTTTC AAGGACATCT CTGACATGCA TGGCAGGGGG 
AACCACAACA GCAAGAAGGA CGGTACGGAA GACAAGAGGC ACAGGAGGAA GAACGGCTCT 
CGCTTCACCG AGGCCCGCAA ACGTGGCCGT ACCATGTTGT ACCACCTGAC CAGCCAGTTC 
TCAAGGGGAG GAAAACGGGA GGTCAACCTG GGTGGAAGCA TGATCGACAA GCCTTCCATG 
CCGGAGTACA AGGTGGCAGC GGTGCAGAAG TCACGTTTCA TTCTGCTCCA CTACAGTGTG 
TCCAAAGCCC TGTGGGACTG GTTGATCCTC CTCGCCACCT TTTATGTGGC AGTGACCGTG 
CCCTACAATG TCAGCTTCAC GCCATACGAA GACACCGTCA CAGCGGCACG CTCCACCATT 
GTCAGTGATA TCGTGGTGGA AATGCTTTTC ATTCTAGACA TTATCCTGAA CTTCCGCACC 
ACCTATGTAA GCCAGTCAGG TCAGGTGGTT TATGAGTCCC GTTCCATCTG CATTCATTAT 
GTCCGAACCT GGTTCTTTGT GGACCTGGTT GCTGCGCTGC CCTTTGACCT TCTGTATGCC 
TTCAACATCA CAGTGACCTC CTTGGTCCAC CTACTCAAGA CTGTGCGCCT GCTGCGCCTC 
CTTCGCCTGC TTCAGAAGCT GGATCGTTAC TCCCAGTATA GCGCCATGGT GCTCACATTA 
TTAATGTCCA TGTTTGCTCT GCTGGCACAC TGGATGGCCT GCATCTGGTA CATGATTGGC 
CGGAAGGAAA TAGAAACCAA TGAGACGTGG GAGATAGGGT GGCTCCACGA GCTGGGCAAA 
CGTCTCGAGA CACCCTACAT TAACAGCACC CTGGGAGGCC CGACAGTACG TAGCTCCTAC 
ATCGCTGCCC TTTACTTTAC CCTCAGCAGC CTGACCAGCG TGGGCTTTGG TAATGTCTCC 
GCCAACACTG ACGCCGAGAA GATCTTCTCC ATCTGCACCA TGCTTGTCGG TGCACTCATG 
CATGCCTTGG TCTTTGGGAA CGTGACAGCC ATCATCCAGC GTATGTACTC AAGGCGCTCT 
TTGTACCACA CGCGCATGAA GGACCTGAAG GACTTCATCC GCGTCCACCG TCTGCCTCAG 
CAGATCAAAC AGCGCATGCT AGAGTATTTC CAGACAACAT GGTCTGTCAA CAATGGCATA 
GATGCTAATG AGCTCCTGCA CGACTTCCCT GATGAGCTGC GGGCCGACAT CGCCATGCAT 
CTGAATAAGG ACATCCTCCA GCTGCCGGTG TTTAAGGGGG CCAGCCGTGG CTGTCTGCGC 
TCACTCTCCC TCCACATAAA AACCTCCTTC TGCGTCCCTG GAGAGTACCT CATCCGACAG 
GGTGACGCTC TGCATGCCAA CTACTTTGTC TGCTCCGGGT CTCTGGAGGT GCTGAAAGAC 
AGCATGGTGC TGGCTATATT AGGAAAAGGG GATCTCATTG GATCCGATCT GCCCGGGACT 
GACCAGGTAA TCAAGACCAA TGCTGATGTG AAGGCCCTGA CCTACTGTGA CCTTCAATAT 
ATCGGCTTGC GGGGACTGAG AGAGGTGCTG GAACTTTACC CTGAATATGC CAACGTGTTT 
GCCTCAGACA TCTACAACAA CCTTACCTAC AACCTGCGTG AGGGCAGCCA GGATGAGGGC 
CAGAGCAAGC TGTCGAGATC CCCGAGGCTT TCCCTCGAAC CCAGGCTCCC CTCCATCGTG 
GAAACAACAG GAGACAACTC TGATGACTCT TTCCATCTCT CTCCAGCGAC TCGCTCCCGT 
CGCAACCTCC TGCTGCCCAG CCTGAGCAGC CCAGTTCGCC GAACCTCCTT GGGAAACCTT 
CTGGGGGACG AGCTGAGGCA GTTCAATGCC CTGCGTCGCT GCCGTTCACC GAACCTCAGC 
CGCGGCTTTC ACAGCCAGCT CTCTTCCCCT CAGCCGCCAC CAAAAAAAGA CCACAACACG 
CCCTCATTTA CCCCAGCCTC TGTCCAGAGA GGGGCAGACG CTGAGCAAAA ACCCACAAAA 
CTGCTTATCC CAACTGTTAC ATGCTTCGCA CCCCCGGATC TGAGCCCCAG GGTCGTAGAT 
GGAATTGAAG ACAATGGGCA CACGTTCCAT TTCAACGGGG AGGGCAGCGG GCCTAAAGCC 
AGCACAGGAG GCAGCACCAG AGAGTCCTCG CAGGCTAATG CCACTCTGCT GTTAGAGTCA 
GAGGAGGTCA GACAGGGCAT CAGTGAACTC AACCACAAGA TGGAAACATT AAACCAGGAA 
GTATCCGAGC TCACCAAAGG CCTGCACCAC ATAATGCACA TCCTCCAAGC CCATGTGTCT 
GTGCATCACT GTAAATCGTC CCTCCCCTCA TATCCCTACG GCATCCAGAT GATGTCCATA 
CCCGCTACAG TCCCCAGCAC CACTACACCC CTCAACCAGC CCTCGGCATC CACTCTTAAA 
AGCGATCCCA TTAGCCACAT AGCCTGTAGC CTACAGATCA CGCCTTACCA TGATCTCTGG 
AGCTGTAGTG AAACTCCTAA AGCCCAACCA GAAAGCTTTT ATCAGCCTCG ATCACCCACT 
TCATGTCCTG CTCCCTCTGG GTCCCAGCCA GGCTCATGCT GTGCCTCTGA GAGCAACACC 
ACAGACATAT GGACAAGTAC ATCTCTGCTT AGCGTCAGTC CAGGATTTCA GGACGGAAAT 
TCAGGCCTTG CGACTCATAC TGGGCACGAA ACTTCCCACT GCAGGCCTGT AAGTCTGAGC 
ACCGCCGCCA CCATCAGCAA GTCCCAGCCC ACATTGTGCC TACAGCCTCC CGGTGAGGGT 
GATGAACAGT CTTGCCTTCT TTTCGGTGCC CCTGCTGCTC TGTCCACACA CAGCCTGCTA 
GATACTTCAC TGACTTCTTA CCCCCATCTC TGCCTGCCCT CTGACACCAA GGTCAAGCTT 
TCTCCAGCCA CACAGGAGGA CATTTGTGTT CTGTTGTCCA CCCCGGCATC ACAAAATCTG 
ATCCAGGACA CCTCCGTCTT CCAAATCAGT GACTCACACC CCCTAATCCT CCCTGTTTCT 
GCCTCGGCCA CACCGACAGT CGTTCCTGTT GACCCCATCC TGGGCTCAGG GTCACCACAG 
TTGGATCTTG CTGAGCCCCG CCTTCCTCTG GGCGATTCAT CCTCCATGGA CCACAACAGT 
CTGGAGTGTC TGCTGGGAAA GGGGGGCTCT CTTGAGAGCA GAGACAGCGA AAGTGTCTCA 
TCACAAAGAT CCAGCATTGG CCTCCAGACT CAGAGCTCAG AGCAGTCGTG GTGTCTGGAC 
CTGACAGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961302.2
CDS	554..4225
Translation	MPVMKGLLAPQNTFLDAIATRFDGTHSNFLLGNAQGHRGYPIVYCSDGFCELTGFTRTEVMQKNCGCHFLFGADTSEHVAQQMLKALEGREEYQAEVYFYKKNGVAFWCLLDIVPIKNEKGEMVLFLFSFKDISDMHGRGNHNSKKDGTEDKRHRRKNGSRFTEARKRGRTMLYHLTSQFSRGGKREVNLGGSMIDKPSMPEYKVAAVQKSRFILLHYSVSKALWDWLILLATFYVAVTVPYNVSFTPYEDTVTAARSTIVSDIVVEMLFILDIILNFRTTYVSQSGQVVYESRSICIHYVRTWFFVDLVAALPFDLLYAFNITVTSLVHLLKTVRLLRLLRLLQKLDRYSQYSAMVLTLLMSMFALLAHWMACIWYMIGRKEIETNETWEIGWLHELGKRLETPYINSTLGGPTVRSSYIAALYFTLSSLTSVGFGNVSANTDAEKIFSICTMLVGALMHALVFGNVTAIIQRMYSRRSLYHTRMKDLKDFIRVHRLPQQIKQRMLEYFQTTWSVNNGIDANELLHDFPDELRADIAMHLNKDILQLPVFKGASRGCLRSLSLHIKTSFCVPGEYLIRQGDALHANYFVCSGSLEVLKDSMVLAILGKGDLIGSDLPGTDQVIKTNADVKALTYCDLQYIGLRGLREVLELYPEYANVFASDIYNNLTYNLREGSQDEGQSKLSRSPRLSLEPRLPSIVETTGDNSDDSFHLSPATRSRRNLLLPSLSSPVRRTSLGNLLGDELRQFNALRRCRSPNLSRGFHSQLSSPQPPPKKDHNTPSFTPASVQRGADAEQKPTKLLIPTVTCFAPPDLSPRVVDGIEDNGHTFHFNGEGSGPKASTGGSTRESSQANATLLLESEEVRQGISELNHKMETLNQEVSELTKGLHHIMHILQAHVSVHHCKSSLPSYPYGIQMMSIPATVPSTTTPLNQPSASTLKSDPISHIACSLQITPYHDLWSCSETPKAQPESFYQPRSPTSCPAPSGSQPGSCCASESNTTDIWTSTSLLSVSPGFQDGNSGLATHTGHETSHCRPVSLSTAATISKSQPTLCLQPPGEGDEQSCLLFGAPAALSTHSLLDTSLTSYPHLCLPSDTKVKLSPATQEDICVLLSTPASQNLIQDTSVFQISDSHPLILPVSASATPTVVPVDPILGSGSPQLDLAEPRLPLGDSSSMDHNSLECLLGKGGSLESRDSESVSSQRSSIGLQTQSSEQSWCLDLTD

Target ORF information:

RefSeq Version	XM_003961253.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes potassium voltage-gated channel subfamily H member 4-like (LOC101063711), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961253.2

ORF Insert Sequence:

ATGCCGGTGA TGAAGGGCCT CCTGGCCCCA CAGAACACCT TTCTGGACGC CATCGCGACA 
CGCTTTGATG GCACCCACAG TAACTTCCTG TTGGGTAACG CCCAGGGCCA CCGCGGCTAC 
CCTATTGTCT ACTGCTCCGA TGGCTTCTGT GAACTGACGG GCTTCACGAG AACCGAGGTG 
ATGCAGAAGA ACTGCGGCTG CCACTTCCTT TTCGGGGCTG ACACCAGCGA GCATGTGGCG 
CAGCAGATGT TGAAGGCTCT GGAGGGTCGC GAAGAGTACC AGGCCGAGGT CTACTTCTAC 
AAGAAAAATG GTGTGGCCTT CTGGTGTCTG CTAGACATTG TGCCCATAAA GAATGAGAAA 
GGCGAAATGG TCCTCTTCCT CTTTTCTTTC AAGGACATCT CTGACATGCA TGGCAGGGGG 
AACCACAACA GCAAGAAGGA CGGTACGGAA GACAAGAGGC ACAGGAGGAA GAACGGCTCT 
CGCTTCACCG AGGCCCGCAA ACGTGGCCGT ACCATGTTGT ACCACCTGAC CAGCCAGTTC 
TCAAGGGGAG GAAAACGGGA GGTCAACCTG GGTGGAAGCA TGATCGACAA GCCTTCCATG 
CCGGAGTACA AGGTGGCAGC GGTGCAGAAG TCACGTTTCA TTCTGCTCCA CTACAGTGTG 
TCCAAAGCCC TGTGGGACTG GTTGATCCTC CTCGCCACCT TTTATGTGGC AGTGACCGTG 
CCCTACAATG TCAGCTTCAC GCCATACGAA GACACCGTCA CAGCGGCACG CTCCACCATT 
GTCAGTGATA TCGTGGTGGA AATGCTTTTC ATTCTAGACA TTATCCTGAA CTTCCGCACC 
ACCTATGTAA GCCAGTCAGG TCAGGTGGTT TATGAGTCCC GTTCCATCTG CATTCATTAT 
GTCCGAACCT GGTTCTTTGT GGACCTGGTT GCTGCGCTGC CCTTTGACCT TCTGTATGCC 
TTCAACATCA CAGTGACCTC CTTGGTCCAC CTACTCAAGA CTGTGCGCCT GCTGCGCCTC 
CTTCGCCTGC TTCAGAAGCT GGATCGTTAC TCCCAGTATA GCGCCATGGT GCTCACATTA 
TTAATGTCCA TGTTTGCTCT GCTGGCACAC TGGATGGCCT GCATCTGGTA CATGATTGGC 
CGGAAGGAAA TAGAAACCAA TGAGACGTGG GAGATAGGGT GGCTCCACGA GCTGGGCAAA 
CGTCTCGAGA CACCCTACAT TAACAGCACC CTGGGAGGCC CGACAGTACG TAGCTCCTAC 
ATCGCTGCCC TTTACTTTAC CCTCAGCAGC CTGACCAGCG TGGGCTTTGG TAATGTCTCC 
GCCAACACTG ACGCCGAGAA GATCTTCTCC ATCTGCACCA TGCTTGTCGG TGCACTCATG 
CATGCCTTGG TCTTTGGGAA CGTGACAGCC ATCATCCAGC GTATGTACTC AAGGCGCTCT 
TTGTACCACA CGCGCATGAA GGACCTGAAG GACTTCATCC GCGTCCACCG TCTGCCTCAG 
CAGATCAAAC AGCGCATGCT AGAGTATTTC CAGACAACAT GGTCTGTCAA CAATGGCATA 
GATGCTAATG AGCTCCTGCA CGACTTCCCT GATGAGCTGC GGGCCGACAT CGCCATGCAT 
CTGAATAAGG ACATCCTCCA GCTGCCGGTG TTTAAGGGGG CCAGCCGTGG CTGTCTGCGC 
TCACTCTCCC TCCACATAAA AACCTCCTTC TGCGTCCCTG GAGAGTACCT CATCCGACAG 
GGTGACGCTC TGCATGCCAA CTACTTTGTC TGCTCCGGGT CTCTGGAGGT GCTGAAAGAC 
AGCATGGTGC TGGCTATATT AGGAAAAGGG GATCTCATTG GATCCGATCT GCCCGGGACT 
GACCAGGTAA TCAAGACCAA TGCTGATGTG AAGGCCCTGA CCTACTGTGA CCTTCAATAT 
ATCGGCTTGC GGGGACTGAG AGAGGTGCTG GAACTTTACC CTGAATATGC CAACGTGTTT 
GCCTCAGACA TCTACAACAA CCTTACCTAC AACCTGCGTG AGGGCAGCCA GGATGAGGGC 
CAGAGCAAGC TGTCGAGATC CCCGAGGCTT TCCCTCGAAC CCAGGCTCCC CTCCATCGTG 
GAAACAACAG GAGACAACTC TGATGACTCT TTCCATCTCT CTCCAGCGAC TCGCTCCCGT 
CGCAACCTCC TGCTGCCCAG CCTGAGCAGC CCAGTTCGCC GAACCTCCTT GGGAAACCTT 
CTGGGGGACG AGCTGAGGCA GTTCAATGCC CTGCGTCGCT GCCGTTCACC GAACCTCAGC 
CGCGGCTTTC ACAGCCAGCT CTCTTCCCCT CAGCCGCCAC CAAAAAAAGA CCACAACACG 
CCCTCATTTA CCCCAGCCTC TGTCCAGAGA GGGGCAGACG CTGAGCAAAA ACCCACAAAA 
CTGCTTATCC CAACTGTTAC ATGCTTCGCA CCCCCGGATC TGAGCCCCAG GGTCGTAGAT 
GGAATTGAAG ACAATGGGCA CACGTTCCAT TTCAACGGGG AGGGCAGCGG GCCTAAAGCC 
AGCACAGGAG GCAGCACCAG AGAGTCCTCG CAGGCTAATG CCACTCTGCT GTTAGAGTCA 
GAGGAGGTCA GACAGGGCAT CAGTGAACTC AACCACAAGA TGGAAACATT AAACCAGGAA 
GTATCCGAGC TCACCAAAGG CCTGCACCAC ATAATGCACA TCCTCCAAGC CCATGTGTCT 
GTGCATCACT GTAAATCGTC CCTCCCCTCA TATCCCTACG GCATCCAGAT GATGTCCATA 
CCCGCTACAG TCCCCAGCAC CACTACACCC CTCAACCAGC CCTCGGCATC CACTCTTAAA 
AGCGATCCCA TTAGCCACAT AGCCTGTAGC CTACAGATCA CGCCTTACCA TGATCTCTGG 
AGCTGTAGTG AAACTCCTAA AGCCCAACCA GAAAGCTTTT ATCAGCCTCG ATCACCCACT 
TCATGTCCTG CTCCCTCTGG GTCCCAGCCA GGCTCATGCT GTGCCTCTGA GAGCAACACC 
ACAGACATAT GGACAAGTAC ATCTCTGCTT AGCGTCAGTC CAGGATTTCA GGACGGAAAT 
TCAGGCCTTG CGACTCATAC TGGGCACGAA ACTTCCCACT GCAGGCCTGT AAGTCTGAGC 
ACCGCCGCCA CCATCAGCAA GTCCCAGCCC ACATTGTGCC TACAGCCTCC CGGTGAGGGT 
GATGAACAGT CTTGCCTTCT TTTCGGTGCC CCTGCTGCTC TGTCCACACA CAGCCTGCTA 
GATACTTCAC TGACTTCTTA CCCCCATCTC TGCCTGCCCT CTGACACCAA GGTCAAGCTT 
TCTCCAGCCA CACAGGAGGA CATTTGTGTT CTGTTGTCCA CCCCGGCATC ACAAAATCTG 
ATCCAGGACA CCTCCGTCTT CCAAATCAGT GACTCACACC CCCTAATCCT CCCTGTTTCT 
GCCTCGGCCA CACCGACAGT CGTTCCTGTT GACCCCATCC TGGGCTCAGG GTCACCACAG 
TTGGATCTTG CTGAGCCCCG CCTTCCTCTG GGCGATTCAT CCTCCATGGA CCACAACAGT 
CTGGAGTGTC TGCTGGGAAA GGGGGGCTCT CTTGAGAGCA GAGACAGCGA AAGTGTCTCA 
TCACAAAGAT CCAGCATTGG CCTCCAGACT CAGAGCTCAG AGCAGTCGTG GTGTCTGGAC 
CTGACAGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101063711 ( XM_003961253.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003961302.1 Takifugu rubripes potassium voltage-gated channel subfamily H member 4-like (LOC101063711), mRNA.

Price & Availability↑

CloneID	OTo00303
Clone ID Related Accession (Same CDS sequence)	XM_003961253.1
Accession Version	XM_003961253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	potassium voltage-gated channel subfamily H member 4-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070899.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCCGGTGA TGAAGGGCCT CCTGGCCCCA CAGAACACCT TTCTGGACGC CATCGCGACA 
CGCTTTGATG GCACCCACAG TAACTTCCTG TTGGGTAACG CCCAGGGCCA CCGCGGCTAC 
CCTATTGTCT ACTGCTCCGA TGGCTTCTGT GAACTGACGG GCTTCACGAG AACCGAGGTG 
ATGCAGAAGA ACTGCGGCTG CCACTTCCTT TTCGGGGCTG ACACCAGCGA GCATGTGGCG 
CAGCAGATGT TGAAGGCTCT GGAGGGTCGC GAAGAGTACC AGGCCGAGGT CTACTTCTAC 
AAGAAAAATG GTGTGGCCTT CTGGTGTCTG CTAGACATTG TGCCCATAAA GAATGAGAAA 
GGCGAAATGG TCCTCTTCCT CTTTTCTTTC AAGGACATCT CTGACATGCA TGGCAGGGGG 
AACCACAACA GCAAGAAGGA CGGTACGGAA GACAAGAGGC ACAGGAGGAA GAACGGCTCT 
CGCTTCACCG AGGCCCGCAA ACGTGGCCGT ACCATGTTGT ACCACCTGAC CAGCCAGTTC 
TCAAGGGGAG GAAAACGGGA GGTCAACCTG GGTGGAAGCA TGATCGACAA GCCTTCCATG 
CCGGAGTACA AGGTGGCAGC GGTGCAGAAG TCACGTTTCA TTCTGCTCCA CTACAGTGTG 
TCCAAAGCCC TGTGGGACTG GTTGATCCTC CTCGCCACCT TTTATGTGGC AGTGACCGTG 
CCCTACAATG TCAGCTTCAC GCCATACGAA GACACCGTCA CAGCGGCACG CTCCACCATT 
GTCAGTGATA TCGTGGTGGA AATGCTTTTC ATTCTAGACA TTATCCTGAA CTTCCGCACC 
ACCTATGTAA GCCAGTCAGG TCAGGTGGTT TATGAGTCCC GTTCCATCTG CATTCATTAT 
GTCCGAACCT GGTTCTTTGT GGACCTGGTT GCTGCGCTGC CCTTTGACCT TCTGTATGCC 
TTCAACATCA CAGTGACCTC CTTGGTCCAC CTACTCAAGA CTGTGCGCCT GCTGCGCCTC 
CTTCGCCTGC TTCAGAAGCT GGATCGTTAC TCCCAGTATA GCGCCATGGT GCTCACATTA 
TTAATGTCCA TGTTTGCTCT GCTGGCACAC TGGATGGCCT GCATCTGGTA CATGATTGGC 
CGGAAGGAAA TAGAAACCAA TGAGACGTGG GAGATAGGGT GGCTCCACGA GCTGGGCAAA 
CGTCTCGAGA CACCCTACAT TAACAGCACC CTGGGAGGCC CGACAGTACG TAGCTCCTAC 
ATCGCTGCCC TTTACTTTAC CCTCAGCAGC CTGACCAGCG TGGGCTTTGG TAATGTCTCC 
GCCAACACTG ACGCCGAGAA GATCTTCTCC ATCTGCACCA TGCTTGTCGG TGCACTCATG 
CATGCCTTGG TCTTTGGGAA CGTGACAGCC ATCATCCAGC GTATGTACTC AAGGCGCTCT 
TTGTACCACA CGCGCATGAA GGACCTGAAG GACTTCATCC GCGTCCACCG TCTGCCTCAG 
CAGATCAAAC AGCGCATGCT AGAGTATTTC CAGACAACAT GGTCTGTCAA CAATGGCATA 
GATGCTAATG AGCTCCTGCA CGACTTCCCT GATGAGCTGC GGGCCGACAT CGCCATGCAT 
CTGAATAAGG ACATCCTCCA GCTGCCGGTG TTTAAGGGGG CCAGCCGTGG CTGTCTGCGC 
TCACTCTCCC TCCACATAAA AACCTCCTTC TGCGTCCCTG GAGAGTACCT CATCCGACAG 
GGTGACGCTC TGCATGCCAA CTACTTTGTC TGCTCCGGGT CTCTGGAGGT GCTGAAAGAC 
AGCATGGTGC TGGCTATATT AGGAAAAGGG GATCTCATTG GATCCGATCT GCCCGGGACT 
GACCAGGTAA TCAAGACCAA TGCTGATGTG AAGGCCCTGA CCTACTGTGA CCTTCAATAT 
ATCGGCTTGC GGGGACTGAG AGAGGTGCTG GAACTTTACC CTGAATATGC CAACGTGTTT 
GCCTCAGACA TCTACAACAA CCTTACCTAC AACCTGCGTG AGGGCAGCCA GGATGAGGGC 
CAGAGCAAGC TGTCGAGATC CCCGAGGCTT TCCCTCGAAC CCAGGCTCCC CTCCATCGTG 
GAAACAACAG GAGACAACTC TGATGACTCT TTCCATCTCT CTCCAGCGAC TCGCTCCCGT 
CGCAACCTCC TGCTGCCCAG CCTGAGCAGC CCAGTTCGCC GAACCTCCTT GGGAAACCTT 
CTGGGGGACG AGCTGAGGCA GTTCAATGCC CTGCGTCGCT GCCGTTCACC GAACCTCAGC 
CGCGGCTTTC ACAGCCAGCT CTCTTCCCCT CAGCCGCCAC CAAAAAAAGA CCACAACACG 
CCCTCATTTA CCCCAGCCTC TGTCCAGAGA GGGGCAGACG CTGAGCAAAA ACCCACAAAA 
CTGCTTATCC CAACTGTTAC ATGCTTCGGA CCCCCGGATC TGAGCCCCAG GGTCGTAGAT 
GGAATTGAAG ACAATGGGCA CACGTTCCAT TTCAACGGGG AGGGCAGCGG GCCTAAAGCC 
AGCACAGGAG GCAGCACCAG AGAGTCCTCG CAGGCTAATG CCACTCTGCT GTTAGAGTCA 
GAGGAGGTCA GACAGGGCAT CAGTGAACTC AACCACAAGA TGGAAACATT AAACCAGGAA 
GTATCCGAGC TCACCAAAGG CCTGCACCAC ATAATGCACA TCCTCCAAGC CCATGTGTCT 
GTGCATCACT GTAAATCGTC CCTCCCCTCA TATCCCTACG GCATCCAGAT GATGTCCATA 
CCCGCTACAG TCCCCAGCAC CACTACACCC CTCAACCAGC CCTCGGCATC CACTCTTAAA 
AGCGATCCCA TTAGCCACAT AGCCTGTAGC CTACAGATCA CGCCTTACCA TGATCTCTGG 
AGCTGTAGTG AAACTCCTAA AGCCCAACCA GAAAGCTTTT ATCAGCCTCG ATCACCCACT 
TCATGTCCTG CTCCCTCTGG GTCCCAGCCA GGCTCATGCT GTGCCTCTGA GAGCAACACC 
ACAGACATAT GGACGAGTAC ATCTCTGCTT AGCGTCAGTC CAGGATTTCA GGACGGAAAT 
TCAGGCCTTG CGACTCATAC TGGGCACGAA ACTTCCCACT GCAGGCCTGT AAGTCTGAGC 
ACCGCCGCCA CCATCAGCAA GTCCCAGCCC ACATTGTGCC TACAGCCTCC CGGTGAGGGT 
GATGAACAGT CTTGCCTTCT TTTCGGTGCC CCTGCTGCTC TGTCCACACA CAGCCTGCTA 
GATACTTCAC TGACTTCTTA CCCCCATCTC TGCCTGCCCT CTGACACCAA GGTCAAGCTT 
TCTCCAGCCA CACAGGAGGA CATTTGTGTT CTGTTGTCCA CCCCGGCATC ACAAAATCTG 
ATCCAGGACA CCTCCGTCTT CCAAATCAGT GACTCACACC CCCTAATCCT CCCTGTTTCT 
GCCTCGGCCA CACCGACAGT CGTTCCTGTT GACCCCATCC TGGGCTCAGG GTCACCACAG 
TTGGATCTTG CTGAGCCCCG CCTTCCTCTG GGCGATTCAT CCTCCATGGA CCACAACAGT 
CTGGAGTGTC TGCTGGGAAA GGGGGGCTCT CTTGAGAGCA GAGACAGCGA AAGTGTCTCA 
TCACAAAGAT CCAGCATTGG CCTCCAGACT CAGAGCTCAG AGCAGTCGTG GTGTCTGGAC 
CTGACAGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003961302.1
CDS	1..3672
Translation	MPVMKGLLAPQNTFLDAIATRFDGTHSNFLLGNAQGHRGYPIVYCSDGFCELTGFTRTEVMQKNCGCHFLFGADTSEHVAQQMLKALEGREEYQAEVYFYKKNGVAFWCLLDIVPIKNEKGEMVLFLFSFKDISDMHGRGNHNSKKDGTEDKRHRRKNGSRFTEARKRGRTMLYHLTSQFSRGGKREVNLGGSMIDKPSMPEYKVAAVQKSRFILLHYSVSKALWDWLILLATFYVAVTVPYNVSFTPYEDTVTAARSTIVSDIVVEMLFILDIILNFRTTYVSQSGQVVYESRSICIHYVRTWFFVDLVAALPFDLLYAFNITVTSLVHLLKTVRLLRLLRLLQKLDRYSQYSAMVLTLLMSMFALLAHWMACIWYMIGRKEIETNETWEIGWLHELGKRLETPYINSTLGGPTVRSSYIAALYFTLSSLTSVGFGNVSANTDAEKIFSICTMLVGALMHALVFGNVTAIIQRMYSRRSLYHTRMKDLKDFIRVHRLPQQIKQRMLEYFQTTWSVNNGIDANELLHDFPDELRADIAMHLNKDILQLPVFKGASRGCLRSLSLHIKTSFCVPGEYLIRQGDALHANYFVCSGSLEVLKDSMVLAILGKGDLIGSDLPGTDQVIKTNADVKALTYCDLQYIGLRGLREVLELYPEYANVFASDIYNNLTYNLREGSQDEGQSKLSRSPRLSLEPRLPSIVETTGDNSDDSFHLSPATRSRRNLLLPSLSSPVRRTSLGNLLGDELRQFNALRRCRSPNLSRGFHSQLSSPQPPPKKDHNTPSFTPASVQRGADAEQKPTKLLIPTVTCFGPPDLSPRVVDGIEDNGHTFHFNGEGSGPKASTGGSTRESSQANATLLLESEEVRQGISELNHKMETLNQEVSELTKGLHHIMHILQAHVSVHHCKSSLPSYPYGIQMMSIPATVPSTTTPLNQPSASTLKSDPISHIACSLQITPYHDLWSCSETPKAQPESFYQPRSPTSCPAPSGSQPGSCCASESNTTDIWTSTSLLSVSPGFQDGNSGLATHTGHETSHCRPVSLSTAATISKSQPTLCLQPPGEGDEQSCLLFGAPAALSTHSLLDTSLTSYPHLCLPSDTKVKLSPATQEDICVLLSTPASQNLIQDTSVFQISDSHPLILPVSASATPTVVPVDPILGSGSPQLDLAEPRLPLGDSSSMDHNSLECLLGKGGSLESRDSESVSSQRSSIGLQTQSSEQSWCLDLTD

Target ORF information:

RefSeq Version	XM_003961253.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes potassium voltage-gated channel subfamily H member 4-like (LOC101063711), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003961253.1

ORF Insert Sequence:

ATGCCGGTGA TGAAGGGCCT CCTGGCCCCA CAGAACACCT TTCTGGACGC CATCGCGACA 
CGCTTTGATG GCACCCACAG TAACTTCCTG TTGGGTAACG CCCAGGGCCA CCGCGGCTAC 
CCTATTGTCT ACTGCTCCGA TGGCTTCTGT GAACTGACGG GCTTCACGAG AACCGAGGTG 
ATGCAGAAGA ACTGCGGCTG CCACTTCCTT TTCGGGGCTG ACACCAGCGA GCATGTGGCG 
CAGCAGATGT TGAAGGCTCT GGAGGGTCGC GAAGAGTACC AGGCCGAGGT CTACTTCTAC 
AAGAAAAATG GTGTGGCCTT CTGGTGTCTG CTAGACATTG TGCCCATAAA GAATGAGAAA 
GGCGAAATGG TCCTCTTCCT CTTTTCTTTC AAGGACATCT CTGACATGCA TGGCAGGGGG 
AACCACAACA GCAAGAAGGA CGGTACGGAA GACAAGAGGC ACAGGAGGAA GAACGGCTCT 
CGCTTCACCG AGGCCCGCAA ACGTGGCCGT ACCATGTTGT ACCACCTGAC CAGCCAGTTC 
TCAAGGGGAG GAAAACGGGA GGTCAACCTG GGTGGAAGCA TGATCGACAA GCCTTCCATG 
CCGGAGTACA AGGTGGCAGC GGTGCAGAAG TCACGTTTCA TTCTGCTCCA CTACAGTGTG 
TCCAAAGCCC TGTGGGACTG GTTGATCCTC CTCGCCACCT TTTATGTGGC AGTGACCGTG 
CCCTACAATG TCAGCTTCAC GCCATACGAA GACACCGTCA CAGCGGCACG CTCCACCATT 
GTCAGTGATA TCGTGGTGGA AATGCTTTTC ATTCTAGACA TTATCCTGAA CTTCCGCACC 
ACCTATGTAA GCCAGTCAGG TCAGGTGGTT TATGAGTCCC GTTCCATCTG CATTCATTAT 
GTCCGAACCT GGTTCTTTGT GGACCTGGTT GCTGCGCTGC CCTTTGACCT TCTGTATGCC 
TTCAACATCA CAGTGACCTC CTTGGTCCAC CTACTCAAGA CTGTGCGCCT GCTGCGCCTC 
CTTCGCCTGC TTCAGAAGCT GGATCGTTAC TCCCAGTATA GCGCCATGGT GCTCACATTA 
TTAATGTCCA TGTTTGCTCT GCTGGCACAC TGGATGGCCT GCATCTGGTA CATGATTGGC 
CGGAAGGAAA TAGAAACCAA TGAGACGTGG GAGATAGGGT GGCTCCACGA GCTGGGCAAA 
CGTCTCGAGA CACCCTACAT TAACAGCACC CTGGGAGGCC CGACAGTACG TAGCTCCTAC 
ATCGCTGCCC TTTACTTTAC CCTCAGCAGC CTGACCAGCG TGGGCTTTGG TAATGTCTCC 
GCCAACACTG ACGCCGAGAA GATCTTCTCC ATCTGCACCA TGCTTGTCGG TGCACTCATG 
CATGCCTTGG TCTTTGGGAA CGTGACAGCC ATCATCCAGC GTATGTACTC AAGGCGCTCT 
TTGTACCACA CGCGCATGAA GGACCTGAAG GACTTCATCC GCGTCCACCG TCTGCCTCAG 
CAGATCAAAC AGCGCATGCT AGAGTATTTC CAGACAACAT GGTCTGTCAA CAATGGCATA 
GATGCTAATG AGCTCCTGCA CGACTTCCCT GATGAGCTGC GGGCCGACAT CGCCATGCAT 
CTGAATAAGG ACATCCTCCA GCTGCCGGTG TTTAAGGGGG CCAGCCGTGG CTGTCTGCGC 
TCACTCTCCC TCCACATAAA AACCTCCTTC TGCGTCCCTG GAGAGTACCT CATCCGACAG 
GGTGACGCTC TGCATGCCAA CTACTTTGTC TGCTCCGGGT CTCTGGAGGT GCTGAAAGAC 
AGCATGGTGC TGGCTATATT AGGAAAAGGG GATCTCATTG GATCCGATCT GCCCGGGACT 
GACCAGGTAA TCAAGACCAA TGCTGATGTG AAGGCCCTGA CCTACTGTGA CCTTCAATAT 
ATCGGCTTGC GGGGACTGAG AGAGGTGCTG GAACTTTACC CTGAATATGC CAACGTGTTT 
GCCTCAGACA TCTACAACAA CCTTACCTAC AACCTGCGTG AGGGCAGCCA GGATGAGGGC 
CAGAGCAAGC TGTCGAGATC CCCGAGGCTT TCCCTCGAAC CCAGGCTCCC CTCCATCGTG 
GAAACAACAG GAGACAACTC TGATGACTCT TTCCATCTCT CTCCAGCGAC TCGCTCCCGT 
CGCAACCTCC TGCTGCCCAG CCTGAGCAGC CCAGTTCGCC GAACCTCCTT GGGAAACCTT 
CTGGGGGACG AGCTGAGGCA GTTCAATGCC CTGCGTCGCT GCCGTTCACC GAACCTCAGC 
CGCGGCTTTC ACAGCCAGCT CTCTTCCCCT CAGCCGCCAC CAAAAAAAGA CCACAACACG 
CCCTCATTTA CCCCAGCCTC TGTCCAGAGA GGGGCAGACG CTGAGCAAAA ACCCACAAAA 
CTGCTTATCC CAACTGTTAC ATGCTTCGGA CCCCCGGATC TGAGCCCCAG GGTCGTAGAT 
GGAATTGAAG ACAATGGGCA CACGTTCCAT TTCAACGGGG AGGGCAGCGG GCCTAAAGCC 
AGCACAGGAG GCAGCACCAG AGAGTCCTCG CAGGCTAATG CCACTCTGCT GTTAGAGTCA 
GAGGAGGTCA GACAGGGCAT CAGTGAACTC AACCACAAGA TGGAAACATT AAACCAGGAA 
GTATCCGAGC TCACCAAAGG CCTGCACCAC ATAATGCACA TCCTCCAAGC CCATGTGTCT 
GTGCATCACT GTAAATCGTC CCTCCCCTCA TATCCCTACG GCATCCAGAT GATGTCCATA 
CCCGCTACAG TCCCCAGCAC CACTACACCC CTCAACCAGC CCTCGGCATC CACTCTTAAA 
AGCGATCCCA TTAGCCACAT AGCCTGTAGC CTACAGATCA CGCCTTACCA TGATCTCTGG 
AGCTGTAGTG AAACTCCTAA AGCCCAACCA GAAAGCTTTT ATCAGCCTCG ATCACCCACT 
TCATGTCCTG CTCCCTCTGG GTCCCAGCCA GGCTCATGCT GTGCCTCTGA GAGCAACACC 
ACAGACATAT GGACGAGTAC ATCTCTGCTT AGCGTCAGTC CAGGATTTCA GGACGGAAAT 
TCAGGCCTTG CGACTCATAC TGGGCACGAA ACTTCCCACT GCAGGCCTGT AAGTCTGAGC 
ACCGCCGCCA CCATCAGCAA GTCCCAGCCC ACATTGTGCC TACAGCCTCC CGGTGAGGGT 
GATGAACAGT CTTGCCTTCT TTTCGGTGCC CCTGCTGCTC TGTCCACACA CAGCCTGCTA 
GATACTTCAC TGACTTCTTA CCCCCATCTC TGCCTGCCCT CTGACACCAA GGTCAAGCTT 
TCTCCAGCCA CACAGGAGGA CATTTGTGTT CTGTTGTCCA CCCCGGCATC ACAAAATCTG 
ATCCAGGACA CCTCCGTCTT CCAAATCAGT GACTCACACC CCCTAATCCT CCCTGTTTCT 
GCCTCGGCCA CACCGACAGT CGTTCCTGTT GACCCCATCC TGGGCTCAGG GTCACCACAG 
TTGGATCTTG CTGAGCCCCG CCTTCCTCTG GGCGATTCAT CCTCCATGGA CCACAACAGT 
CTGGAGTGTC TGCTGGGAAA GGGGGGCTCT CTTGAGAGCA GAGACAGCGA AAGTGTCTCA 
TCACAAAGAT CCAGCATTGG CCTCCAGACT CAGAGCTCAG AGCAGTCGTG GTGTCTGGAC 
CTGACAGACT AG