washc4 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	washc4
Entrez Gene ID	101063207
Full Name	WASH complex subunit 4
Synonyms	kiaa1033
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003967378.2	XP_003967427.2	WASH complex subunit 4

Pathways from BioSystems
Gene Ontology

The following washc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the washc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo20206	XM_003967378.2 Latest version!	Takifugu rubripes WASH complex subunit 4 (washc4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

washc4 ( XM_003967378.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003967427.2 Takifugu rubripes WASH complex subunit 4 (washc4), mRNA.

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CloneID	OTo20206
Clone ID Related Accession (Same CDS sequence)	XM_003967378.2
Accession Version	XM_003967378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	WASH complex subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003967378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTAG AAACGATGAC ACATGACTGG GAGTTTGATC GTTTTGACGA CGGTTCACAG 
AAAATACACA CAGAGGTTCA GCTGAAGAAC TACAGCAAGT TTCTGGAGGA ATACACATCC 
CAGCTCAGAG GCATTGAGGA GGCTCTGGAT GACACCATTG GAGATGTCTG GGACTTCACC 
CTGGACCCCA TTGCCCTAAA ACTTCTCCCT TATGAGCAGT CTTCTATATT GGAATTAATA 
AAAACAGACA ATAAGGTTTT AAATAAAGTC ATAACAGTTT ATGCTGCTCT CTGCAGTGAA 
GTGAAAAAGC TTAAGTATGA GGCAGAGACC AAGTTCTACA GTGGTTTATT GTACTATGGA 
GAGGGAGTGT CTGACACCAG TATTGTTGAA GGAGAGCTGC AGATTCAAAT GGGCAGATTC 
ATCTCATTCC TTCAGGAGTT ATCATGTTTT GTATCAAGAT GTTATGAAGT GGTGGTCAAC 
ATTGTTCACC AGCTGGCTGC CCTCTACAAT AACAAGGGTC CACCAAAGGT TATTGAGTCA 
TCTGGTGTCC ATTTTCAGGT CGTATACGAA CACCTTGGGG AGTTGTTGGT TGTGCTTCTT 
ACACTGGATG AAATTATGGA AAATCATGCC ACACTGAAGG ATCATTGGAA GATGTATAAA 
AGGTTGTTAA AATCTGTGCA CCACAACCCT GGAAAGTTCT CTATCCCTGA AGAGAAGCTG 
AAACCTTTTG AGAAACTTCT GCTTAGGCTT GAGGGACAAC TCCTGGATGG CATGATACTG 
CAGGCCTGTT TGGAGCAGAG GTTCGATGCT GAAGTGGTCA CTGTCTCAAA AAACGGTGCC 
TTTGCTGAGG AGTTTGCCTT CAATATTCGC ACCATCTTCA CTAATGTTGA GTCAAAAATT 
GGTGAACCAT CGGAGATTGA CCAGAGAGAT AAATATTCTC TCATCTGCGG TCTGTTTGTG 
CTTCACTTCC ACATTTTCAG GACTGTGGAC AAGAAGCTTT ACAAATCATT GCTGGATATC 
TGTAAAAAGG TTCCCGCCAT CACACTGACT GCAAACATCA TCTGGTTTCC TGACACTTTT 
CTTATCAATA AGGTCCCAGC CGCAGCGAAA CTGATGGATA AGAAAAGTCT GCAAAGCATC 
CGAGCACAGA GAGACGCCTA CCTTCTGCAA CGATCTCAGA CACTGACAAA GGACGTTCAG 
TCTTATTACG TGTTTGTGGC ATCGTGGATG ATGAAGATGG AGTCCATATT GTCCAAAGAG 
CATCAAAACG ACAAGCTGAC AGAAGACCTT AACAGCAGAT GTAATGTGTT CGTGCAGGGT 
GTCCTGTATG CATATAGCAT CGGCACCATT ATTAGAACCA CAATGAACAT GCACATGTCA 
ATGCAGCGGC CCATGACCAA AACGTCTGTC AAAGCTCTGT GTCGCCTGGT GGAATTGCTC 
AAGGCAGTGG AGCACACATT CCACAGGCGG TCCATGGTTG TAGCAGACTC TGTCTCTCAC 
ATCACCCAGC AGCTGCAGTC ACAGGCCCTC AACGCCATAG GTGTCGCCAA GAAAAGGGTG 
ATCTCTGACA AAAAGTACAG CGAACAGCGG CTCGATGTTC TTTCCAGTTT GGTGCTGGCA 
GAAAACGCTC TTAATGGACC GAGCACAAAG GAGCGGCTGC TGGTGGTGTT CCTGGCACTG 
TGTGTCGGCA CTCAGCTGAA AACCTTTAAA GACGAAGAGC TGCTGCCGCT GCAGCTTGTT 
CTGAAGAAGT TGGAACTGAT CAGCGAGTTA AGGGAAAGGG TCAAGCTGCA GTGCGACTGT 
AGCTTCCTTT ACTGGCACCG AGCTGTGTTT CCCATCTACC TAGATGATGT GTATGACAAT 
GCTGTAGATG CAGCACGAAT ACATTACATG TTTAGTGCAC TGAGGGACAG CGTGCCCTCA 
ATGTTGCATG CCAAACACAT GGAGTCATGT GATCAGCTGC TGGAGAGCTA TGACAAAGAG 
ATCATGGACG TGTTCAACGA GCACTTGCTG GACAAGCTGT GTAAGGAGAT CGAGAAAGAC 
CTGCGTCTGT CTGTGCACAC CCACCTGAAG CTGGACGACA GGAACCCTTT CAAAGTCGGC 
ATGAAGGACC TGGCTCACTT CTTCTCCCTC AAACCTATTC GATTCTTCAA CCGCTTCATC 
CACATCAAAG CCTACGTGAC CCACTATCTA GACAAGACCT TCTACAACCT GACCACGGTA 
GCGCTCCACG ACTGGGCCAC CTACAGTGAG ATGAGAAACC TGGCCACGCA GCGCTACGGG 
CTCCCCATGA CGGAGGCACA CCTGCCTAGC CAGACCCTGG AGCAGGGTTT GGATGTACTG 
GAGATCATGA GGAATATTCA CGTCTTTGTC TCACGGTACC TTTATAACCT CAACAACCAG 
ATTTTCATTG AGAAGGCAAG TAACAATAAG CACTTGAACA CCATCAACAT CCGTCACATC 
GCCAACTCCA TCCGGACCCA CGGCACAGGC ATAATGAACA CCACGGTTAA TTTTACCTAC 
CAGTTCCTGC GGAAGAAGTT TTACATATTC AGCCAGTTCA TGTACGATGA GCACATCAAG 
TCCAGACTCA TCAAGGACAT CCGATTCTAC AGAGAAACAA AGGATCAATC TGATCAGAAG 
TATCCATTTG AGCGAGCAGA GAAGTTTAAC CGTGGCATCA GGAAGCTGGG CATCACTCCT 
GATGGACAGA GCTACCTGGA CCAGTTCAGA CAGCTCATCA GCCATATCGG GAACGCCATG 
GGCTACGTCC GAATGATCCG CTCCGGAGGC CTCCATTGTT GCAGCAGTGC TATCAGGTTT 
GTTCCCGACC TTGAGGACAT CGTCAACTTT GAAGAACTGG TGAAGGAGGA AGGATTATCG 
GAGGAGACAC AGAGAGCTGC CAGTGTTCTA GATTCAGTTT TGGGGGACTT GACCAGCAAC 
TCTGCAGAGG GGACTGAGTA TTTCAAGATG CTGGTTGCTG TATTTGCCCC CGAGTTCCGC 
AGTGCCAAAA ACATGCACCT GAGGAACTTC TACATGATTG TACCCCCACT GACGGTGAAC 
TTCGTGGAAC ATTCCATCAG CTGCAAAGAA AAACTCAACA AGAAGAACAA AACTGGAGCT 
GCTTTCACAG ACGATGGCTT TGCAATGGGT GTGGCCTACA TACTGAAGCT GCTGGACCAG 
TACTTGGAGT TTGACTCATT GCACTGGTTC CAGTCCGTCA GAGATAAGTA CAGGAAAGAA 
ATTAACGCCG TGGTGAAGGA GCAGAACATC CAATCCACAA GCCAGGATGA AAAGTTGCTG 
CAAACGATGA ATCTCACTCA GAAGCGGCTG GACATATACC TACAGGAGTT TGAGCTGCTC 
TACTTCTCAC TAAGCAGTGC CCGGATCTTC TTCAGAGCAG ACAAGACGGC GGCCGAGGAG 
TCCCTGGAGA AGAAAGACAA GGATGAAGCT GCCAGAGGAG GGGCTTCAGC CAGTTCCACA 
CCGGCCGAGC CCGCCACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003967427.2
CDS	87..3590
Translation	MAVETMTHDWEFDRFDDGSQKIHTEVQLKNYSKFLEEYTSQLRGIEEALDDTIGDVWDFTLDPIALKLLPYEQSSILELIKTDNKVLNKVITVYAALCSEVKKLKYEAETKFYSGLLYYGEGVSDTSIVEGELQIQMGRFISFLQELSCFVSRCYEVVVNIVHQLAALYNNKGPPKVIESSGVHFQVVYEHLGELLVVLLTLDEIMENHATLKDHWKMYKRLLKSVHHNPGKFSIPEEKLKPFEKLLLRLEGQLLDGMILQACLEQRFDAEVVTVSKNGAFAEEFAFNIRTIFTNVESKIGEPSEIDQRDKYSLICGLFVLHFHIFRTVDKKLYKSLLDICKKVPAITLTANIIWFPDTFLINKVPAAAKLMDKKSLQSIRAQRDAYLLQRSQTLTKDVQSYYVFVASWMMKMESILSKEHQNDKLTEDLNSRCNVFVQGVLYAYSIGTIIRTTMNMHMSMQRPMTKTSVKALCRLVELLKAVEHTFHRRSMVVADSVSHITQQLQSQALNAIGVAKKRVISDKKYSEQRLDVLSSLVLAENALNGPSTKERLLVVFLALCVGTQLKTFKDEELLPLQLVLKKLELISELRERVKLQCDCSFLYWHRAVFPIYLDDVYDNAVDAARIHYMFSALRDSVPSMLHAKHMESCDQLLESYDKEIMDVFNEHLLDKLCKEIEKDLRLSVHTHLKLDDRNPFKVGMKDLAHFFSLKPIRFFNRFIHIKAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYGLPMTEAHLPSQTLEQGLDVLEIMRNIHVFVSRYLYNLNNQIFIEKASNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTYQFLRKKFYIFSQFMYDEHIKSRLIKDIRFYRETKDQSDQKYPFERAEKFNRGIRKLGITPDGQSYLDQFRQLISHIGNAMGYVRMIRSGGLHCCSSAIRFVPDLEDIVNFEELVKEEGLSEETQRAASVLDSVLGDLTSNSAEGTEYFKMLVAVFAPEFRSAKNMHLRNFYMIVPPLTVNFVEHSISCKEKLNKKNKTGAAFTDDGFAMGVAYILKLLDQYLEFDSLHWFQSVRDKYRKEINAVVKEQNIQSTSQDEKLLQTMNLTQKRLDIYLQEFELLYFSLSSARIFFRADKTAAEESLEKKDKDEAARGGASASSTPAEPATK

Target ORF information:

RefSeq Version	XM_003967378.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes WASH complex subunit 4 (washc4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003967378.2

ORF Insert Sequence:

ATGGCGGTAG AAACGATGAC ACATGACTGG GAGTTTGATC GTTTTGACGA CGGTTCACAG 
AAAATACACA CAGAGGTTCA GCTGAAGAAC TACAGCAAGT TTCTGGAGGA ATACACATCC 
CAGCTCAGAG GCATTGAGGA GGCTCTGGAT GACACCATTG GAGATGTCTG GGACTTCACC 
CTGGACCCCA TTGCCCTAAA ACTTCTCCCT TATGAGCAGT CTTCTATATT GGAATTAATA 
AAAACAGACA ATAAGGTTTT AAATAAAGTC ATAACAGTTT ATGCTGCTCT CTGCAGTGAA 
GTGAAAAAGC TTAAGTATGA GGCAGAGACC AAGTTCTACA GTGGTTTATT GTACTATGGA 
GAGGGAGTGT CTGACACCAG TATTGTTGAA GGAGAGCTGC AGATTCAAAT GGGCAGATTC 
ATCTCATTCC TTCAGGAGTT ATCATGTTTT GTATCAAGAT GTTATGAAGT GGTGGTCAAC 
ATTGTTCACC AGCTGGCTGC CCTCTACAAT AACAAGGGTC CACCAAAGGT TATTGAGTCA 
TCTGGTGTCC ATTTTCAGGT CGTATACGAA CACCTTGGGG AGTTGTTGGT TGTGCTTCTT 
ACACTGGATG AAATTATGGA AAATCATGCC ACACTGAAGG ATCATTGGAA GATGTATAAA 
AGGTTGTTAA AATCTGTGCA CCACAACCCT GGAAAGTTCT CTATCCCTGA AGAGAAGCTG 
AAACCTTTTG AGAAACTTCT GCTTAGGCTT GAGGGACAAC TCCTGGATGG CATGATACTG 
CAGGCCTGTT TGGAGCAGAG GTTCGATGCT GAAGTGGTCA CTGTCTCAAA AAACGGTGCC 
TTTGCTGAGG AGTTTGCCTT CAATATTCGC ACCATCTTCA CTAATGTTGA GTCAAAAATT 
GGTGAACCAT CGGAGATTGA CCAGAGAGAT AAATATTCTC TCATCTGCGG TCTGTTTGTG 
CTTCACTTCC ACATTTTCAG GACTGTGGAC AAGAAGCTTT ACAAATCATT GCTGGATATC 
TGTAAAAAGG TTCCCGCCAT CACACTGACT GCAAACATCA TCTGGTTTCC TGACACTTTT 
CTTATCAATA AGGTCCCAGC CGCAGCGAAA CTGATGGATA AGAAAAGTCT GCAAAGCATC 
CGAGCACAGA GAGACGCCTA CCTTCTGCAA CGATCTCAGA CACTGACAAA GGACGTTCAG 
TCTTATTACG TGTTTGTGGC ATCGTGGATG ATGAAGATGG AGTCCATATT GTCCAAAGAG 
CATCAAAACG ACAAGCTGAC AGAAGACCTT AACAGCAGAT GTAATGTGTT CGTGCAGGGT 
GTCCTGTATG CATATAGCAT CGGCACCATT ATTAGAACCA CAATGAACAT GCACATGTCA 
ATGCAGCGGC CCATGACCAA AACGTCTGTC AAAGCTCTGT GTCGCCTGGT GGAATTGCTC 
AAGGCAGTGG AGCACACATT CCACAGGCGG TCCATGGTTG TAGCAGACTC TGTCTCTCAC 
ATCACCCAGC AGCTGCAGTC ACAGGCCCTC AACGCCATAG GTGTCGCCAA GAAAAGGGTG 
ATCTCTGACA AAAAGTACAG CGAACAGCGG CTCGATGTTC TTTCCAGTTT GGTGCTGGCA 
GAAAACGCTC TTAATGGACC GAGCACAAAG GAGCGGCTGC TGGTGGTGTT CCTGGCACTG 
TGTGTCGGCA CTCAGCTGAA AACCTTTAAA GACGAAGAGC TGCTGCCGCT GCAGCTTGTT 
CTGAAGAAGT TGGAACTGAT CAGCGAGTTA AGGGAAAGGG TCAAGCTGCA GTGCGACTGT 
AGCTTCCTTT ACTGGCACCG AGCTGTGTTT CCCATCTACC TAGATGATGT GTATGACAAT 
GCTGTAGATG CAGCACGAAT ACATTACATG TTTAGTGCAC TGAGGGACAG CGTGCCCTCA 
ATGTTGCATG CCAAACACAT GGAGTCATGT GATCAGCTGC TGGAGAGCTA TGACAAAGAG 
ATCATGGACG TGTTCAACGA GCACTTGCTG GACAAGCTGT GTAAGGAGAT CGAGAAAGAC 
CTGCGTCTGT CTGTGCACAC CCACCTGAAG CTGGACGACA GGAACCCTTT CAAAGTCGGC 
ATGAAGGACC TGGCTCACTT CTTCTCCCTC AAACCTATTC GATTCTTCAA CCGCTTCATC 
CACATCAAAG CCTACGTGAC CCACTATCTA GACAAGACCT TCTACAACCT GACCACGGTA 
GCGCTCCACG ACTGGGCCAC CTACAGTGAG ATGAGAAACC TGGCCACGCA GCGCTACGGG 
CTCCCCATGA CGGAGGCACA CCTGCCTAGC CAGACCCTGG AGCAGGGTTT GGATGTACTG 
GAGATCATGA GGAATATTCA CGTCTTTGTC TCACGGTACC TTTATAACCT CAACAACCAG 
ATTTTCATTG AGAAGGCAAG TAACAATAAG CACTTGAACA CCATCAACAT CCGTCACATC 
GCCAACTCCA TCCGGACCCA CGGCACAGGC ATAATGAACA CCACGGTTAA TTTTACCTAC 
CAGTTCCTGC GGAAGAAGTT TTACATATTC AGCCAGTTCA TGTACGATGA GCACATCAAG 
TCCAGACTCA TCAAGGACAT CCGATTCTAC AGAGAAACAA AGGATCAATC TGATCAGAAG 
TATCCATTTG AGCGAGCAGA GAAGTTTAAC CGTGGCATCA GGAAGCTGGG CATCACTCCT 
GATGGACAGA GCTACCTGGA CCAGTTCAGA CAGCTCATCA GCCATATCGG GAACGCCATG 
GGCTACGTCC GAATGATCCG CTCCGGAGGC CTCCATTGTT GCAGCAGTGC TATCAGGTTT 
GTTCCCGACC TTGAGGACAT CGTCAACTTT GAAGAACTGG TGAAGGAGGA AGGATTATCG 
GAGGAGACAC AGAGAGCTGC CAGTGTTCTA GATTCAGTTT TGGGGGACTT GACCAGCAAC 
TCTGCAGAGG GGACTGAGTA TTTCAAGATG CTGGTTGCTG TATTTGCCCC CGAGTTCCGC 
AGTGCCAAAA ACATGCACCT GAGGAACTTC TACATGATTG TACCCCCACT GACGGTGAAC 
TTCGTGGAAC ATTCCATCAG CTGCAAAGAA AAACTCAACA AGAAGAACAA AACTGGAGCT 
GCTTTCACAG ACGATGGCTT TGCAATGGGT GTGGCCTACA TACTGAAGCT GCTGGACCAG 
TACTTGGAGT TTGACTCATT GCACTGGTTC CAGTCCGTCA GAGATAAGTA CAGGAAAGAA 
ATTAACGCCG TGGTGAAGGA GCAGAACATC CAATCCACAA GCCAGGATGA AAAGTTGCTG 
CAAACGATGA ATCTCACTCA GAAGCGGCTG GACATATACC TACAGGAGTT TGAGCTGCTC 
TACTTCTCAC TAAGCAGTGC CCGGATCTTC TTCAGAGCAG ACAAGACGGC GGCCGAGGAG 
TCCCTGGAGA AGAAAGACAA GGATGAAGCT GCCAGAGGAG GGGCTTCAGC CAGTTCCACA 
CCGGCCGAGC CCGCCACAAA GTGA