LOC101061951 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101061951
Entrez Gene ID	101061951
Full Name	transcription intermediary factor 1-alpha-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011607612.1	XP_011605914.1	transcription intermediary factor 1-alpha-like
XM_029842214.1	XP_029698074.1	E3 ubiquitin-protein ligase TRIM33-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101061951 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101061951 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo19236	XM_011607612.1 Latest version!	Takifugu rubripes transcription intermediary factor 1-alpha-like (LOC101061951), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo57961	XM_029842214.1 Latest version!	Takifugu rubripes E3 ubiquitin-protein ligase TRIM33-like (LOC101061951), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101061951 ( XM_011607612.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605914.1 Takifugu rubripes transcription intermediary factor 1-alpha-like (LOC101061951), mRNA.

Price & Availability↑

CloneID	OTo19236
Clone ID Related Accession (Same CDS sequence)	XM_011607612.1
Accession Version	XM_011607612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	transcription intermediary factor 1-alpha-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071016.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 15% of CDS bases ##RefSeq-Attributes-END##

ATGCGAGAAA ATGACTCTTG CGCCTTGCTG CTCTCACCTG TGCGGGCGCA CGTCGCACGA 
CACCATCTGA AAGTGCAGCA TATGGATGCC TGCGAAGAAC AGGAGGAGGC AGCGGGGGCT 
TCTGCTGCAG CCGCCGCCGT GCTTTTAGAG AACGAAGCTG AGAGCATGCA AGTGCAAGAA 
GAAAAGTGCG CTGCAGCTGA GCTAAACAAT CTAAACTCGT GTCCCGTTTG CTGCCTGAAC 
TTCTACAGTC GGGAGCCTAA ACTTCTTCCG TGCCTGCACT CGTTTTGCAA GAAATGTTTG 
CCCACGCCGT CGAGGAATTT GGCCATGGTC GAGGCGCCAA ACTCCCGGGT GGACGGTGTG 
AAACCACTGA ATGTGATCCG CTGTCCGGTG TGCAGACAGG AGTGTATGGA AGTGGACGTA 
ATGGAAAATG TTTTTGTGGA GGACTCAGTC GAAGCTCCCA GCAGCACAGT GGAAAGATCA 
GCTCAGATCT GCATGACCTG TGATGACAAC ACCGAGGCGG TTGGGTTTTG TGTAATCTGT 
GTCGAGTACC TGTGCTCTGC CTGTGTGGAG GCGCACCAAA GGGTCAAATT TACCAAAGAT 
CACACTATCA CCCAGAAGGA AGAAGTATCA GAAGAAATCC ACGGATGGTC AACTCAGAGG 
CCGATGTTCT GTGGTATCCA CAAACAAGAG CCGGTGAAGT TGTTCTGTGA GACCTGTGAC 
CTGCTGACCT GCCGTGACTG TCAGCTGGTC AGGCACAAAG ACCACAGGTA TCATTTCCTT 
GAGGATGCTT ATAAGTATCA CAAGCAGCAT GTAGACAGCA TGACCCATCA GCTGCAGGAC 
AAAAGGATGC TCATTGAAGA GGTGTCCTCT GCCATAAACA ACAGGCTTCT CCAGGCGGAG 
GAGAACCGCA CATCGGTTGA GACTGAAATA AAAAACTCCA TCTGCAGCTT GATTGCGGAG 
ATGAACAAAA AGGGCCAGAT GCTGTTAAAT CAACTCGAGA GTGTAACAAA GGATCACAAG 
AGGGTTTTAC AAAAACAGCA GGAGGACATC GGCTATCTGT CCAGACACCT GGATCACGTC 
ATCGGCTTCA CCAAGTGGGC CACCGCCAGG AACAAGGGCA CGGCCTTCCT GCACTGTAAA 
CGTTTAATTC TCTTCCAGAT TGGAAACCTC CTGCAGGCAA AGTGCAGCAC ATCATTTGTG 
CCGCAGACTG TCATACGCTT TCAGGGTCGC TCCCCAGTCT CTTCCAGTCT TGATCTGGGC 
TCTCTGCTGG TGGAGAGCAC ACCAGGCAAA CGGCTGCCAG GTTTTCAGGG TATTCCTCAT 
GATGTCAAGA ACCCTAGGGA AGGACCCTCG GGCTCCCCCT ACGGTTTTGC CATCGGAAAC 
CCCCGGAACA CTCTGGCTCT GCTCCAAATG GAGGTAGAGA AGCTAAACCC AACGACCCAC 
TGGCAAGCCC AGCCGCCTTG GGGATGGTGC CAGAGCGTCA GGCTGCCCCG GTCCGGGCCC 
CAGACCCTTC TCCAACGTGC TCCCTCCCAC AACGTGGCTG CCCAACCACA TTGCAGGTTT 
ATGGTGCCTC CTCCGAGCAA CACCGGCCCG ACCAGCGGCC CACCAAACCC TGGGTTTAAC 
CCGCAGCATT ATAGGTTGTT GGGTAGCAGC TCCGGCTACC AATCTAAACC TGGAGGTACG 
TTCTCTTCAC CTCTTTACTC ATCACCCGTG CCCATCGGAG ACGTCGCTCT GCCTCAGCCA 
CGACACACGG CGAGTGACAT CATCCATTAT ATGAAAGCTG AGTCACCATA TCTGAATCAA 
AGGACTGATC CAGTATATGC CACTACGAGA AATCCACCAC AGACCCCTGT GTCTTGTGTG 
CCATGTACAG ATGTGCAAGG GAAGCAGAAC CCAGCAGGGC TCGCTGCTGT TTCCCAGGAG 
GAGAATTCAG AATCTGCTTC CTGGGAACCT GCAGAAACAC ATCAGACCAT TGGAGACGAA 
GGCCCATCTG TCAGGAAGAG ACCGAGACCA TCACCAGGTC CCATCGTAGT CATCAAAGAT 
GAACCAGATG ACGACATTAG CTATGACACC AAAAGGACAA ACCTCCCTGA CAGCACAGGT 
GACCAACCAG ACCTGCTGAC CCAAGTGAGC AGCCAAGACA GGGAGGACGT CTCCACCCGT 
CCAAGAGTCA CTGATAAAGT CCAGATTCCC CTGCACACTC ATAGCGCACC CCAAGACCAC 
AGCAACAGCA ACGGAAATGA TCATAACAGT TTGCTCGAAG GAGAGACGCA GCGTAAAGCT 
CCGTTAAAGC GCTCTGACAG AGGCGTGTGC ACCGTGTGTC AGACAGGGGG GGAGCTGTTG 
CTCTGCGATA AGTGCTCCAA GCATTTTCAC CTTTTTTGCC ACATTCCAGC ACTCCGAAAC 
GTTCCAAGTG GCTGTTGGTC GTGTTCCTTC TGCCTTGATT CCCCTGAGAT GGGCTCCAAA 
TGCAAACCCA AAGCCAGAGG AGTGAAGACG GAGTCGGAGT CTGAGGGAGG ATTTCTTCCC 
GAGGAAAAGA GAAAATGTGA GAGACTGTTG CTGCACTTGT TTTGCAGCCC AGACTTCGGA 
GAATCCCCCA CGGTGTGTGC CAATAATAGG AAGAATGGGA GTGGACCATC AATGCTTCAC 
ACTGTGAGGA AACGACTGGA AGCGACAGAG AGTCTGCGCT ACAAAAACCT GGCAGAGTTT 
GTGTCAGATG TCAGGCTGGC CTGCAGGAAC TGGGCCACTA AGCCTGAACC AGGAGCTACC 
GCGGCATCCA AGGGGCTTAA TGAGAAGTTT GAAGAACGCC TGAAGAACGT CTTCCCAGAA 
CACACTTTTC CTGAGATGAA ATCTCCGTCC ACTCCCGACT CGGTCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605914.1
CDS	1..2871
Translation	MRENDSCALLLSPVRAHVARHHLKVQHMDACEEQEEAAGASAAAAAVLLENEAESMQVQEEKCAAAELNNLNSCPVCCLNFYSREPKLLPCLHSFCKKCLPTPSRNLAMVEAPNSRVDGVKPLNVIRCPVCRQECMEVDVMENVFVEDSVEAPSSTVERSAQICMTCDDNTEAVGFCVICVEYLCSACVEAHQRVKFTKDHTITQKEEVSEEIHGWSTQRPMFCGIHKQEPVKLFCETCDLLTCRDCQLVRHKDHRYHFLEDAYKYHKQHVDSMTHQLQDKRMLIEEVSSAINNRLLQAEENRTSVETEIKNSICSLIAEMNKKGQMLLNQLESVTKDHKRVLQKQQEDIGYLSRHLDHVIGFTKWATARNKGTAFLHCKRLILFQIGNLLQAKCSTSFVPQTVIRFQGRSPVSSSLDLGSLLVESTPGKRLPGFQGIPHDVKNPREGPSGSPYGFAIGNPRNTLALLQMEVEKLNPTTHWQAQPPWGWCQSVRLPRSGPQTLLQRAPSHNVAAQPHCRFMVPPPSNTGPTSGPPNPGFNPQHYRLLGSSSGYQSKPGGTFSSPLYSSPVPIGDVALPQPRHTASDIIHYMKAESPYLNQRTDPVYATTRNPPQTPVSCVPCTDVQGKQNPAGLAAVSQEENSESASWEPAETHQTIGDEGPSVRKRPRPSPGPIVVIKDEPDDDISYDTKRTNLPDSTGDQPDLLTQVSSQDREDVSTRPRVTDKVQIPLHTHSAPQDHSNSNGNDHNSLLEGETQRKAPLKRSDRGVCTVCQTGGELLLCDKCSKHFHLFCHIPALRNVPSGCWSCSFCLDSPEMGSKCKPKARGVKTESESEGGFLPEEKRKCERLLLHLFCSPDFGESPTVCANNRKNGSGPSMLHTVRKRLEATESLRYKNLAEFVSDVRLACRNWATKPEPGATAASKGLNEKFEERLKNVFPEHTFPEMKSPSTPDSVS

Target ORF information:

RefSeq Version	XM_011607612.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes transcription intermediary factor 1-alpha-like (LOC101061951), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607612.1

ORF Insert Sequence:

ATGCGAGAAA ATGACTCTTG CGCCTTGCTG CTCTCACCTG TGCGGGCGCA CGTCGCACGA 
CACCATCTGA AAGTGCAGCA TATGGATGCC TGCGAAGAAC AGGAGGAGGC AGCGGGGGCT 
TCTGCTGCAG CCGCCGCCGT GCTTTTAGAG AACGAAGCTG AGAGCATGCA AGTGCAAGAA 
GAAAAGTGCG CTGCAGCTGA GCTAAACAAT CTAAACTCGT GTCCCGTTTG CTGCCTGAAC 
TTCTACAGTC GGGAGCCTAA ACTTCTTCCG TGCCTGCACT CGTTTTGCAA GAAATGTTTG 
CCCACGCCGT CGAGGAATTT GGCCATGGTC GAGGCGCCAA ACTCCCGGGT GGACGGTGTG 
AAACCACTGA ATGTGATCCG CTGTCCGGTG TGCAGACAGG AGTGTATGGA AGTGGACGTA 
ATGGAAAATG TTTTTGTGGA GGACTCAGTC GAAGCTCCCA GCAGCACAGT GGAAAGATCA 
GCTCAGATCT GCATGACCTG TGATGACAAC ACCGAGGCGG TTGGGTTTTG TGTAATCTGT 
GTCGAGTACC TGTGCTCTGC CTGTGTGGAG GCGCACCAAA GGGTCAAATT TACCAAAGAT 
CACACTATCA CCCAGAAGGA AGAAGTATCA GAAGAAATCC ACGGATGGTC AACTCAGAGG 
CCGATGTTCT GTGGTATCCA CAAACAAGAG CCGGTGAAGT TGTTCTGTGA GACCTGTGAC 
CTGCTGACCT GCCGTGACTG TCAGCTGGTC AGGCACAAAG ACCACAGGTA TCATTTCCTT 
GAGGATGCTT ATAAGTATCA CAAGCAGCAT GTAGACAGCA TGACCCATCA GCTGCAGGAC 
AAAAGGATGC TCATTGAAGA GGTGTCCTCT GCCATAAACA ACAGGCTTCT CCAGGCGGAG 
GAGAACCGCA CATCGGTTGA GACTGAAATA AAAAACTCCA TCTGCAGCTT GATTGCGGAG 
ATGAACAAAA AGGGCCAGAT GCTGTTAAAT CAACTCGAGA GTGTAACAAA GGATCACAAG 
AGGGTTTTAC AAAAACAGCA GGAGGACATC GGCTATCTGT CCAGACACCT GGATCACGTC 
ATCGGCTTCA CCAAGTGGGC CACCGCCAGG AACAAGGGCA CGGCCTTCCT GCACTGTAAA 
CGTTTAATTC TCTTCCAGAT TGGAAACCTC CTGCAGGCAA AGTGCAGCAC ATCATTTGTG 
CCGCAGACTG TCATACGCTT TCAGGGTCGC TCCCCAGTCT CTTCCAGTCT TGATCTGGGC 
TCTCTGCTGG TGGAGAGCAC ACCAGGCAAA CGGCTGCCAG GTTTTCAGGG TATTCCTCAT 
GATGTCAAGA ACCCTAGGGA AGGACCCTCG GGCTCCCCCT ACGGTTTTGC CATCGGAAAC 
CCCCGGAACA CTCTGGCTCT GCTCCAAATG GAGGTAGAGA AGCTAAACCC AACGACCCAC 
TGGCAAGCCC AGCCGCCTTG GGGATGGTGC CAGAGCGTCA GGCTGCCCCG GTCCGGGCCC 
CAGACCCTTC TCCAACGTGC TCCCTCCCAC AACGTGGCTG CCCAACCACA TTGCAGGTTT 
ATGGTGCCTC CTCCGAGCAA CACCGGCCCG ACCAGCGGCC CACCAAACCC TGGGTTTAAC 
CCGCAGCATT ATAGGTTGTT GGGTAGCAGC TCCGGCTACC AATCTAAACC TGGAGGTACG 
TTCTCTTCAC CTCTTTACTC ATCACCCGTG CCCATCGGAG ACGTCGCTCT GCCTCAGCCA 
CGACACACGG CGAGTGACAT CATCCATTAT ATGAAAGCTG AGTCACCATA TCTGAATCAA 
AGGACTGATC CAGTATATGC CACTACGAGA AATCCACCAC AGACCCCTGT GTCTTGTGTG 
CCATGTACAG ATGTGCAAGG GAAGCAGAAC CCAGCAGGGC TCGCTGCTGT TTCCCAGGAG 
GAGAATTCAG AATCTGCTTC CTGGGAACCT GCAGAAACAC ATCAGACCAT TGGAGACGAA 
GGCCCATCTG TCAGGAAGAG ACCGAGACCA TCACCAGGTC CCATCGTAGT CATCAAAGAT 
GAACCAGATG ACGACATTAG CTATGACACC AAAAGGACAA ACCTCCCTGA CAGCACAGGT 
GACCAACCAG ACCTGCTGAC CCAAGTGAGC AGCCAAGACA GGGAGGACGT CTCCACCCGT 
CCAAGAGTCA CTGATAAAGT CCAGATTCCC CTGCACACTC ATAGCGCACC CCAAGACCAC 
AGCAACAGCA ACGGAAATGA TCATAACAGT TTGCTCGAAG GAGAGACGCA GCGTAAAGCT 
CCGTTAAAGC GCTCTGACAG AGGCGTGTGC ACCGTGTGTC AGACAGGGGG GGAGCTGTTG 
CTCTGCGATA AGTGCTCCAA GCATTTTCAC CTTTTTTGCC ACATTCCAGC ACTCCGAAAC 
GTTCCAAGTG GCTGTTGGTC GTGTTCCTTC TGCCTTGATT CCCCTGAGAT GGGCTCCAAA 
TGCAAACCCA AAGCCAGAGG AGTGAAGACG GAGTCGGAGT CTGAGGGAGG ATTTCTTCCC 
GAGGAAAAGA GAAAATGTGA GAGACTGTTG CTGCACTTGT TTTGCAGCCC AGACTTCGGA 
GAATCCCCCA CGGTGTGTGC CAATAATAGG AAGAATGGGA GTGGACCATC AATGCTTCAC 
ACTGTGAGGA AACGACTGGA AGCGACAGAG AGTCTGCGCT ACAAAAACCT GGCAGAGTTT 
GTGTCAGATG TCAGGCTGGC CTGCAGGAAC TGGGCCACTA AGCCTGAACC AGGAGCTACC 
GCGGCATCCA AGGGGCTTAA TGAGAAGTTT GAAGAACGCC TGAAGAACGT CTTCCCAGAA 
CACACTTTTC CTGAGATGAA ATCTCCGTCC ACTCCCGACT CGGTCTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101061951 ( XM_029842214.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029698074.1 Takifugu rubripes E3 ubiquitin-protein ligase TRIM33-like (LOC101061951), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo57961
Clone ID Related Accession (Same CDS sequence)	XM_029842214.1
Accession Version	XM_029842214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	E3 ubiquitin-protein ligase TRIM33-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGAAA ATGACTCTTG CGCCTTGCTG CTCTCACCTG TGCGGGCGCA CGTCGCACGA 
CACCATCTGA AAGTGCAGCA TATGGATGCC TGCGAAGAAC AGGAGGAGGC AGCGGGGGCT 
TCTGCTGCAG CCGCCGCCGT GCTTTTAGAG AACGAAGCTG AGAGCATGCA AGTGCAAGAA 
GAAAAGTGCG CTGCAGCTGA GCTAAACAAT CTAAACTCGT GTCCCGTTTG CTGCCTGAAC 
TTCTACAGTC GGGAGCCTAA ACTTCTTCCG TGCCTGCACT CGTTTTGCAA GAAATGTTTG 
CCCACGCCGT CGAGGAATTT GGCCATGGTC GAGGCGCCAA ACTCCCGGGT GGACAGTGTG 
AAACCACTGA ATGTGATCCG CTGTCCGGTG TGCAGACAGG AGTGTATGGA AGTGGACGTA 
ATGGAAAATG TTTTTGTGGA GGACTCAGTC GAAGCTCCCA GCAGCACAGT GGAAAGATCA 
GCTCAGATCT GCATGACCTG TGATGACAAC ACCGAGGCGG TGGGGTTTTG TGTAATCTGT 
GTCGAGTACC TGTGCTCTGC CTGTGTGGAG GCGCACCAAA GGGTCAAATT TACCAAAGAT 
CACACTATCA CACAGAAGGA AGAAGTATCA GAAGAAATCC ACGGATGGTC AACTCAGAGG 
CCGATGTTCT GTGGTATCCA CAAACAAGAG CCGGTGAAGT TGTTCTGTGA GACCTGTGAC 
CTGCTGACCT GCCGTGACTG TCAGCTGGTC AGGCACAAAG ACCACAGGTA TCATTTCCTT 
GAGGATGCTT ATAAGTATCA CAAGCAGCAT GTAGACAGCA TGACCCATCA GCTGCAGGAC 
AAAAGGATGC TCATTGAAGA GGTGTCCTCT GCCATAAACA ACAGGCTTCT CCAGGCGGAG 
GAGAACCGCA CATCGGTTGA GACTGAAATA AAAAACTCCA TCTGCAGCTT GATTGCGGAG 
ATGAACAAAA AGGGCCAGAT GCTGTTAAAT CAACTCGAGA GTGTAACAAA GGATCACAAG 
AGGGTTTTAC AAAAACAGCA GGAGGACATC GGCTATCTGT CCAGACACCT GGATCACGTC 
ATCGGCTTCA CCAAGTGGGC CACCGCCAGG AACAAGGGCA CGGCCTTCCT GCACTGTAAA 
CGTTTAATTC TCTTCCAGAT TGGAAACCTC CTGCAGGCAA AGTGCAGCAC ATCATTTGTG 
CCGCAGACTG TCATACGCTT TCAGGGTCGC TCCCCATATT GGGCTTCCAG TCTTGATCTG 
GGCTCTCTGC TGGTGGAGAG CACACCAGGC AAACGGCTGC CAGGTTTTCA GGGTATTCCT 
CATGATGTCA AGAACCCTAG GGAAGGACCC TCGGGCTCCC CCTACGGTTT TGCCATCGGA 
AACCCCCGGA ACACTCTGGC TCTGCTCCAA ATGGAGGTAG AGAAGCTAAA CCCAACGACC 
CACTGGCAAG CCCAGCCGCC TTGGGGATGG TGCCAGAGCG TCAGGCTGCC CCGGTCCGGG 
CCCCAGACCG TTCTCCAACG TGCTCCCTCC CACAACGTGG GTGCCCAACT ACATTGCAGG 
TTTATGGTGC CTCCTCCGAG CAACACCGGC CCGACCAGCG GCCCACCAAA CCCTGGGTTT 
AACCCGCAGC ATTATAGGTT GTTGGGTAGC AGCTCCGGCT ACCAATCTAA ACCTGGAGGT 
ACGTTCTCTT CACCTCTTTA CTCATCACCC GTGCCCATTG GAGACGTCGC TCTGCCTCAG 
CCACGACACA CGGCTGAGTC ACCATATCTG AATCAAAGGA CTGATCCAGT ATATGCCACT 
ACGAGAAATC CACCACAGAC CCCTGTGTCT TGTGTGCCAT GTACAGATGT GCAAGGGAAG 
CAGAACCCAG CAGGGCTCGC TGCTGTTTCC CAGGAGGAGA ATTCAGAATC TGCTTCCTGG 
GAACCTGCAG AAACACATCA GACCATTGGA GACGAAGGCC CATCTGTCAG GAAGAGACCG 
AGACCATCAC CAGGTCCCAT CGTAGTCATC AAAGATGAAC CAGATGACGA CATTAGCTAT 
GACACCAAAA GGACAAACCT CCCTGACAGC ACAGGTGACC AACCAGACCT GCTGACCCAA 
GTGAGCAGCC AAGACAGGGA GGACGTCTCC ACCCGTCCAA GAGTCACTGA TAAAGTCCAG 
ATGCCCGTGC ACACTCATAG CACACCCCAA GACCACAGCA ACAGCAACGG AAATGATCAT 
AACAGTTTGC TCAAAGGAGA GACGCAGCGT AAAGCTCCGT TAAAGCGCTC TGACAGAGGC 
GTGTGCACCG TGTGTCAGAC AGGGGGGGAG CTGTTGCTCT GCGATAAGTG CTCCAAGCAT 
TTTCACCTTT TTTGCCACAT TCCAGCACTC CGAAACGTTC CAAGTGGCTG TTGGTCGTGT 
TCCTTCTGCC TTGATTCCCC TGAGATGGGC TCCAAATGCA AACCCAAAGC CAGAGGAGTG 
AAGACGGAGT CGGAGTCTGA GGGAGGATTT CTTCCCGAGG AAAAGAGAAA ATGTGAGAGA 
CTGTTGCTGC ACTTGTTTTG CAGCCCAGAC TTCGGAGAAT CCCCCACGGT GTGTGCCAAT 
AATAGGAAGA ATGGGAGTGG ACCATCAATG CTTCACACTG TGAGGAAACG ACTGGAAGCG 
ACAGAGAGTC TGCGCTACAA AAACCTGGCA GAGTTTGTGT CAGATGTCAG GCTGGCCTGC 
AGGAACTGGG CCACTAAGCC TGAACCAGGA GCTACCGCGG CATCCAAGGG GCTTAATGAG 
CAGTTTGAAG AACGCCTGAA GAACGTCTTC CCAGAACACA CTTTTCCTGA GATGAAATCT 
CCGTCCACTC CCGACTCGGT CTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029698074.1
CDS	154..3000
Translation	MRENDSCALLLSPVRAHVARHHLKVQHMDACEEQEEAAGASAAAAAVLLENEAESMQVQEEKCAAAELNNLNSCPVCCLNFYSREPKLLPCLHSFCKKCLPTPSRNLAMVEAPNSRVDSVKPLNVIRCPVCRQECMEVDVMENVFVEDSVEAPSSTVERSAQICMTCDDNTEAVGFCVICVEYLCSACVEAHQRVKFTKDHTITQKEEVSEEIHGWSTQRPMFCGIHKQEPVKLFCETCDLLTCRDCQLVRHKDHRYHFLEDAYKYHKQHVDSMTHQLQDKRMLIEEVSSAINNRLLQAEENRTSVETEIKNSICSLIAEMNKKGQMLLNQLESVTKDHKRVLQKQQEDIGYLSRHLDHVIGFTKWATARNKGTAFLHCKRLILFQIGNLLQAKCSTSFVPQTVIRFQGRSPYWASSLDLGSLLVESTPGKRLPGFQGIPHDVKNPREGPSGSPYGFAIGNPRNTLALLQMEVEKLNPTTHWQAQPPWGWCQSVRLPRSGPQTVLQRAPSHNVGAQLHCRFMVPPPSNTGPTSGPPNPGFNPQHYRLLGSSSGYQSKPGGTFSSPLYSSPVPIGDVALPQPRHTAESPYLNQRTDPVYATTRNPPQTPVSCVPCTDVQGKQNPAGLAAVSQEENSESASWEPAETHQTIGDEGPSVRKRPRPSPGPIVVIKDEPDDDISYDTKRTNLPDSTGDQPDLLTQVSSQDREDVSTRPRVTDKVQMPVHTHSTPQDHSNSNGNDHNSLLKGETQRKAPLKRSDRGVCTVCQTGGELLLCDKCSKHFHLFCHIPALRNVPSGCWSCSFCLDSPEMGSKCKPKARGVKTESESEGGFLPEEKRKCERLLLHLFCSPDFGESPTVCANNRKNGSGPSMLHTVRKRLEATESLRYKNLAEFVSDVRLACRNWATKPEPGATAASKGLNEQFEERLKNVFPEHTFPEMKSPSTPDSVS

Target ORF information:

RefSeq Version	XM_029842214.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes E3 ubiquitin-protein ligase TRIM33-like (LOC101061951), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029842214.1

ORF Insert Sequence:

ATGCGAGAAA ATGACTCTTG CGCCTTGCTG CTCTCACCTG TGCGGGCGCA CGTCGCACGA 
CACCATCTGA AAGTGCAGCA TATGGATGCC TGCGAAGAAC AGGAGGAGGC AGCGGGGGCT 
TCTGCTGCAG CCGCCGCCGT GCTTTTAGAG AACGAAGCTG AGAGCATGCA AGTGCAAGAA 
GAAAAGTGCG CTGCAGCTGA GCTAAACAAT CTAAACTCGT GTCCCGTTTG CTGCCTGAAC 
TTCTACAGTC GGGAGCCTAA ACTTCTTCCG TGCCTGCACT CGTTTTGCAA GAAATGTTTG 
CCCACGCCGT CGAGGAATTT GGCCATGGTC GAGGCGCCAA ACTCCCGGGT GGACAGTGTG 
AAACCACTGA ATGTGATCCG CTGTCCGGTG TGCAGACAGG AGTGTATGGA AGTGGACGTA 
ATGGAAAATG TTTTTGTGGA GGACTCAGTC GAAGCTCCCA GCAGCACAGT GGAAAGATCA 
GCTCAGATCT GCATGACCTG TGATGACAAC ACCGAGGCGG TGGGGTTTTG TGTAATCTGT 
GTCGAGTACC TGTGCTCTGC CTGTGTGGAG GCGCACCAAA GGGTCAAATT TACCAAAGAT 
CACACTATCA CACAGAAGGA AGAAGTATCA GAAGAAATCC ACGGATGGTC AACTCAGAGG 
CCGATGTTCT GTGGTATCCA CAAACAAGAG CCGGTGAAGT TGTTCTGTGA GACCTGTGAC 
CTGCTGACCT GCCGTGACTG TCAGCTGGTC AGGCACAAAG ACCACAGGTA TCATTTCCTT 
GAGGATGCTT ATAAGTATCA CAAGCAGCAT GTAGACAGCA TGACCCATCA GCTGCAGGAC 
AAAAGGATGC TCATTGAAGA GGTGTCCTCT GCCATAAACA ACAGGCTTCT CCAGGCGGAG 
GAGAACCGCA CATCGGTTGA GACTGAAATA AAAAACTCCA TCTGCAGCTT GATTGCGGAG 
ATGAACAAAA AGGGCCAGAT GCTGTTAAAT CAACTCGAGA GTGTAACAAA GGATCACAAG 
AGGGTTTTAC AAAAACAGCA GGAGGACATC GGCTATCTGT CCAGACACCT GGATCACGTC 
ATCGGCTTCA CCAAGTGGGC CACCGCCAGG AACAAGGGCA CGGCCTTCCT GCACTGTAAA 
CGTTTAATTC TCTTCCAGAT TGGAAACCTC CTGCAGGCAA AGTGCAGCAC ATCATTTGTG 
CCGCAGACTG TCATACGCTT TCAGGGTCGC TCCCCATATT GGGCTTCCAG TCTTGATCTG 
GGCTCTCTGC TGGTGGAGAG CACACCAGGC AAACGGCTGC CAGGTTTTCA GGGTATTCCT 
CATGATGTCA AGAACCCTAG GGAAGGACCC TCGGGCTCCC CCTACGGTTT TGCCATCGGA 
AACCCCCGGA ACACTCTGGC TCTGCTCCAA ATGGAGGTAG AGAAGCTAAA CCCAACGACC 
CACTGGCAAG CCCAGCCGCC TTGGGGATGG TGCCAGAGCG TCAGGCTGCC CCGGTCCGGG 
CCCCAGACCG TTCTCCAACG TGCTCCCTCC CACAACGTGG GTGCCCAACT ACATTGCAGG 
TTTATGGTGC CTCCTCCGAG CAACACCGGC CCGACCAGCG GCCCACCAAA CCCTGGGTTT 
AACCCGCAGC ATTATAGGTT GTTGGGTAGC AGCTCCGGCT ACCAATCTAA ACCTGGAGGT 
ACGTTCTCTT CACCTCTTTA CTCATCACCC GTGCCCATTG GAGACGTCGC TCTGCCTCAG 
CCACGACACA CGGCTGAGTC ACCATATCTG AATCAAAGGA CTGATCCAGT ATATGCCACT 
ACGAGAAATC CACCACAGAC CCCTGTGTCT TGTGTGCCAT GTACAGATGT GCAAGGGAAG 
CAGAACCCAG CAGGGCTCGC TGCTGTTTCC CAGGAGGAGA ATTCAGAATC TGCTTCCTGG 
GAACCTGCAG AAACACATCA GACCATTGGA GACGAAGGCC CATCTGTCAG GAAGAGACCG 
AGACCATCAC CAGGTCCCAT CGTAGTCATC AAAGATGAAC CAGATGACGA CATTAGCTAT 
GACACCAAAA GGACAAACCT CCCTGACAGC ACAGGTGACC AACCAGACCT GCTGACCCAA 
GTGAGCAGCC AAGACAGGGA GGACGTCTCC ACCCGTCCAA GAGTCACTGA TAAAGTCCAG 
ATGCCCGTGC ACACTCATAG CACACCCCAA GACCACAGCA ACAGCAACGG AAATGATCAT 
AACAGTTTGC TCAAAGGAGA GACGCAGCGT AAAGCTCCGT TAAAGCGCTC TGACAGAGGC 
GTGTGCACCG TGTGTCAGAC AGGGGGGGAG CTGTTGCTCT GCGATAAGTG CTCCAAGCAT 
TTTCACCTTT TTTGCCACAT TCCAGCACTC CGAAACGTTC CAAGTGGCTG TTGGTCGTGT 
TCCTTCTGCC TTGATTCCCC TGAGATGGGC TCCAAATGCA AACCCAAAGC CAGAGGAGTG 
AAGACGGAGT CGGAGTCTGA GGGAGGATTT CTTCCCGAGG AAAAGAGAAA ATGTGAGAGA 
CTGTTGCTGC ACTTGTTTTG CAGCCCAGAC TTCGGAGAAT CCCCCACGGT GTGTGCCAAT 
AATAGGAAGA ATGGGAGTGG ACCATCAATG CTTCACACTG TGAGGAAACG ACTGGAAGCG 
ACAGAGAGTC TGCGCTACAA AAACCTGGCA GAGTTTGTGT CAGATGTCAG GCTGGCCTGC 
AGGAACTGGG CCACTAAGCC TGAACCAGGA GCTACCGCGG CATCCAAGGG GCTTAATGAG 
CAGTTTGAAG AACGCCTGAA GAACGTCTTC CCAGAACACA CTTTTCCTGA GATGAAATCT 
CCGTCCACTC CCGACTCGGT CTCTTGA