LOC100265327 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100265327
Entrez Gene ID	100265327
Full Name	mediator of RNA polymerase II transcription subunit 16
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010648925.1	XP_010647227.1	mediator of RNA polymerase II transcription subunit 16

Pathways from BioSystems
Gene Ontology

The following LOC100265327 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100265327 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OVa32605	XM_010648925.1 Latest version!	Vitis vinifera mediator of RNA polymerase II transcription subunit 16 (LOC100265327), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100265327 ( XM_010648925.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010647227.1 Vitis vinifera mediator of RNA polymerase II transcription subunit 16 (LOC100265327), mRNA.

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CloneID	OVa32605
Clone ID Related Accession (Same CDS sequence)	XM_010648925.1
Accession Version	XM_010648925.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-21
Organism	Vitis vinifera(wine grape)
Product	mediator of RNA polymerase II transcription subunit 16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003724461.1) and transcript sequence (GAKH01066252.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1606 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGTCCTG GCTCTGGTGC TAAGGACTCT GAGGACGAGC CCGTGCCTCA AGCGCTTGAG 
GTCGCCAAGG GTTTGGAGAA GTCCGAAGTG GTTAGCGTCG ACGAAGAGGA GGGGGACCAG 
GAGGAGGAAG GGGAACAAGT TGTTGAGAAG ACCGCTGACG ATCCCATGGA GGAAGATTCG 
GTCAATCCGG CCACCGTGTT CTGTATCAGG CTCAAGCAGC CTCGGTCTAA TTTGCAGCAC 
AAGATGAGCG TGCCCGAGCT TTGTCGCAAT TTCAGTGCTG TTGCTTGGTG TGGGAAGCTG 
AATGCCATAG CATGTGCTTC AGAAACTTGT GCAAGAATCC CAAGTTCTAA TGCAAATCCC 
CCATTTTGGA TCCCTATACA TATTGTGATA CCAGAGCGGC CGACGGAATG CTCTGTTTTC 
AGTGTCATAG CAGATTCTCC TCGTGACTCT GTTCAGTTTA TTGAATGGTC CCCTGCTTCG 
TGTCCTCGTG CCTTACTAAT AGCTAATTTC CATGGGAGGA TAACTATTTG GACTCAGCCT 
TCTCAAGGAC CAGCTAATCT GGTGCGTGAT GCTAGTTGCT GGGAGCGTGA ATATGAATGG 
CATCAGGATA TTGCTGTTGT TACAAAATGG CTATCAGGGG TGTCTCCGTA TAGGTGGCTT 
TCCTCAAAAA CCAGTAGCTC CACAAGTTTA AAGTCACCTT TTGAGGAAAA ATTCCTTTCT 
CAGCAGCCAC AAACTTCAGA TAGGTGGCCA AATTTTCTGT GTGTTTGCTC CGTCTTCTCA 
TCAGGCTCCA TTCAACTTCA CTGGTCTCAG TGGCCTCCTC AGAATGGTGC AACATCAAAG 
TGGTTTTCCA CAGCCAAAGG ACTATTGGGA GCTGGACCTA GTGGAATTAT GGCTGCTGAT 
GCTATCATAA CAGACACTGG TGCCATGCAT GTTGCAGGTG TTCCGATTGT AAATCCATCG 
ACTGTTGTTG TTTGGGAGGT CACACCAGGC CCTGGAAATG GTTTTCAAGT GATCCCAAAG 
TCAAGTGCGA CCAATGGGGT CCCACCTTCT CTCAATCCTC CTAGTTGGGC AGGTTTTTCC 
CCTTTGGCTG CATATTTATT TAGCTGGCAA GAGTTTTTGG CTTCTGAAAT GAAGCAAGGG 
AGGAAGCAGA CAGATCAAGA TTTCAATGAC GCTGTATTAC TACATTGTTC ACCAGTTTCA 
AATTTTTCTG CATATGTGAG TCCTGAGACT GCAGCTCAAT CAGCAGCAAC TACCACATGG 
GGTTCTGGAG TCACTGCAGT TGCCTTTGAT CCAACTCGTG GAGGTTCAGT GATAGCTGTT 
GCGATAGTTG AAGGGCAGTA CATGTCTCCC TATGATCCAG ATGAGGGCCC TTCAATCACA 
GGATGGAGGG TGCAACGCTG GGAATCATCT CTTCAGCCTG TTGTCCTCCA TCAAATATTT 
GGAAATCCCA CTTCCAGTTT TGGGGGGCAG GCACCTGTGC AAACAGTTTG GCAAACAAAA 
GTCAATAAAA GCATCCCACC AACTAGTGAT TTTAAAAGTC AACAAACACC TGCAACAGGA 
CCAACCTCTG AGAGAAATAC ATCTGATTCT TCTATGGATA TGGGCAAAAG AGTCAGCTTT 
GATCCCTTTG ATTTGCCTAG TGATGTTAGA ACACTTGCTC AAATTTCATA TTCTGCTCAT 
GGTGGTGAGA TTGCTGTTGC TTTTCTAAGG GGTGGGGTGC ACATCTTTTC TGGTCCAAAC 
TTTACACCTG TTGATAACTA CCAGATTAAC GTTGGCTCTG CAATTGCTAC TCCTGCATTC 
TCATCTACAA GCTGCTGTTC AGCTTCTGTT TGGCATGATA CCAACAAGGA CAGAACTATG 
TTGAAGATAA TTCGCGTTCT CCCCCCTGCT GTTCCAAGTA GTCAAGTAAA AGCCAACTCA 
TCAAACTGGG AACGTGCAAT TGCTGAGAGG TTTTGGTGGA GCCTTTTGGT TGGAGTTGAT 
TGGTGGGATG CTGTTGGATG TACACAGAGC GCAGCTGAGG ATGGCATTGT TTCTTTGAAC 
AGTGTCATTG CAGTGTTGGA TGCAGATTTC CATTCTCTCC CTTCTACTCA GCACCGACAA 
CAGTATGGCC CTAGTCTGGA CAGGATAAAA TGCAGACTAT TGGAAGGTAT AAACGCGCAG 
GAGGTTAGGG CAATGGTTCT GGATATGCAA GCAAGGTTAC TGCTGGATAT GCTGGGGAAA 
GGAATTGAAT CAGCGCTAAT GAAACCTTCA GCTCTAGTAC CTGAGCCATG GCAAGCAACT 
GGTGAGACAT TATCTGGCAT TGATCCAGAA GAAATGGCCG TTGACCCAGC ACTAGTTTTT 
AGCATTCAGG CTTATGTTGA TTCCGTCCTT GATCTAGCTT CACACTTCAT CACTCGCTTG 
AGGCGTTATG CAAGTTTCTG TCGTACTTTG GCAAGTCATG CTGTTACTGC AGGCACGGGC 
AGCAATCGAA GTGCAGTGGC TAGTCCTACC CAAAGTTCTG CATCTCCTGC AACAAGTCAG 
GGAGGTCAAA GTGGTACCAC CAGTTCCACA GGGAGCACCC AGATGCAAGC TTGGGTACAA 
GGTGCTATTG CTAAGATTAG TAGCACCACC GATGGAGTTC CAAATTCGAC CCCAAATCCC 
ATAAATGGTC CACCATCCTT CATACCCATA AGTATTAATA CTGGGACATT TCCTGGAACT 
CCAGCTGTTA GGCTTATTGG GGACTGCCAT TTCCTTCATA GATTATGCCA ACTTTTGCTT 
TTTTGTTTTT TCTTCCGACG AACACAATTA CCTCGTGTTA TTGGGGTTGC ACAGAGGAAT 
GCTGATACCA ATATGCATAA ACCCCAAGCT ACTGGGACCA GCAAGGTGGA GGAGAACAGT 
ACTGTTTCTA ACAAACTGGC ATCAGCTATG GCAAGATCGG AAGAGGGTCA GGTTGCTCGA 
GCTAATCATG CCATCAATGG AGCTAAAGGA GCTGAAGAAG GTCCTACAGG CCGGTCAAGA 
TTAGGTTCTG GAAATGCTGG GCAAGGATAC ACATTTGAGG AGGTGAAGGT CCTTTTCCTC 
ATACTTATGG ATCTCTGTCG TAGAACAGCC GCTCTGCAAC ATCCATTGCC AATTTCTCAG 
GTGGGAAGCA ACAACATCCA GGTCCGTCTG CATTACATAG ATGGGAGTTA TACTGTCTTA 
CCAGAGGTTG TAGAAGCATC TCTTGGTCCA CATATGCAGA ATATGCCCCG GCCTAGAGGT 
GCAGATGCTG CTGGTCTTTT ACTTCGTGAG TTAGAGCTCC ATCCTCCTGC TGAAGAGTGG 
CATAGGCGGA ATATGTTTGG TGGGCCCTGG TCTGATCCTG AGGATATGGG TTCTGTAGAT 
GATACTCCCA AACTAAGTAC CTATGCTGAT CCACTTGAAT CAAACTCATT TGAAAATAAT 
GATACATATT ATGGAGCCCG TGGTTTGTGG CCAAGGAAAC GCAGGTTATC TGAAAGAGAT 
GCAGCTTTTG GCTTGAACAC TTCGGTGGGA TTGGGTGCAT ATCTTGGAAT AATGGGATCT 
AGAAGAGATG TTGTCACTGC TGTCTGGAAG ACTGGTCTGG AAGGGGTTTG GTACAAGTGC 
ATAAGATGTT TGCGGCAGAC TTCAGCTTTT GCAGCAGATG CTACCAATCA ACCTAATCAG 
AATGAGCGGG AGATGTGGTG GATCAGCCGT TGGGCTTATG GCTGCCCAAT GTGTGGTGGA 
ACATGGGTTC GGGTTGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010647227.1
CDS	205..3945
Translation	MSPGSGAKDSEDEPVPQALEVAKGLEKSEVVSVDEEEGDQEEEGEQVVEKTADDPMEEDSVNPATVFCIRLKQPRSNLQHKMSVPELCRNFSAVAWCGKLNAIACASETCARIPSSNANPPFWIPIHIVIPERPTECSVFSVIADSPRDSVQFIEWSPASCPRALLIANFHGRITIWTQPSQGPANLVRDASCWEREYEWHQDIAVVTKWLSGVSPYRWLSSKTSSSTSLKSPFEEKFLSQQPQTSDRWPNFLCVCSVFSSGSIQLHWSQWPPQNGATSKWFSTAKGLLGAGPSGIMAADAIITDTGAMHVAGVPIVNPSTVVVWEVTPGPGNGFQVIPKSSATNGVPPSLNPPSWAGFSPLAAYLFSWQEFLASEMKQGRKQTDQDFNDAVLLHCSPVSNFSAYVSPETAAQSAATTTWGSGVTAVAFDPTRGGSVIAVAIVEGQYMSPYDPDEGPSITGWRVQRWESSLQPVVLHQIFGNPTSSFGGQAPVQTVWQTKVNKSIPPTSDFKSQQTPATGPTSERNTSDSSMDMGKRVSFDPFDLPSDVRTLAQISYSAHGGEIAVAFLRGGVHIFSGPNFTPVDNYQINVGSAIATPAFSSTSCCSASVWHDTNKDRTMLKIIRVLPPAVPSSQVKANSSNWERAIAERFWWSLLVGVDWWDAVGCTQSAAEDGIVSLNSVIAVLDADFHSLPSTQHRQQYGPSLDRIKCRLLEGINAQEVRAMVLDMQARLLLDMLGKGIESALMKPSALVPEPWQATGETLSGIDPEEMAVDPALVFSIQAYVDSVLDLASHFITRLRRYASFCRTLASHAVTAGTGSNRSAVASPTQSSASPATSQGGQSGTTSSTGSTQMQAWVQGAIAKISSTTDGVPNSTPNPINGPPSFIPISINTGTFPGTPAVRLIGDCHFLHRLCQLLLFCFFFRRTQLPRVIGVAQRNADTNMHKPQATGTSKVEENSTVSNKLASAMARSEEGQVARANHAINGAKGAEEGPTGRSRLGSGNAGQGYTFEEVKVLFLILMDLCRRTAALQHPLPISQVGSNNIQVRLHYIDGSYTVLPEVVEASLGPHMQNMPRPRGADAAGLLLRELELHPPAEEWHRRNMFGGPWSDPEDMGSVDDTPKLSTYADPLESNSFENNDTYYGARGLWPRKRRLSERDAAFGLNTSVGLGAYLGIMGSRRDVVTAVWKTGLEGVWYKCIRCLRQTSAFAADATNQPNQNEREMWWISRWAYGCPMCGGTWVRVV

Target ORF information:

RefSeq Version	XM_010648925.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera mediator of RNA polymerase II transcription subunit 16 (LOC100265327), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010648925.1

ORF Insert Sequence:

ATGAGTCCTG GCTCTGGTGC TAAGGACTCT GAGGACGAGC CCGTGCCTCA AGCGCTTGAG 
GTCGCCAAGG GTTTGGAGAA GTCCGAAGTG GTTAGCGTCG ACGAAGAGGA GGGGGACCAG 
GAGGAGGAAG GGGAACAAGT TGTTGAGAAG ACCGCTGACG ATCCCATGGA GGAAGATTCG 
GTCAATCCGG CCACCGTGTT CTGTATCAGG CTCAAGCAGC CTCGGTCTAA TTTGCAGCAC 
AAGATGAGCG TGCCCGAGCT TTGTCGCAAT TTCAGTGCTG TTGCTTGGTG TGGGAAGCTG 
AATGCCATAG CATGTGCTTC AGAAACTTGT GCAAGAATCC CAAGTTCTAA TGCAAATCCC 
CCATTTTGGA TCCCTATACA TATTGTGATA CCAGAGCGGC CGACGGAATG CTCTGTTTTC 
AGTGTCATAG CAGATTCTCC TCGTGACTCT GTTCAGTTTA TTGAATGGTC CCCTGCTTCG 
TGTCCTCGTG CCTTACTAAT AGCTAATTTC CATGGGAGGA TAACTATTTG GACTCAGCCT 
TCTCAAGGAC CAGCTAATCT GGTGCGTGAT GCTAGTTGCT GGGAGCGTGA ATATGAATGG 
CATCAGGATA TTGCTGTTGT TACAAAATGG CTATCAGGGG TGTCTCCGTA TAGGTGGCTT 
TCCTCAAAAA CCAGTAGCTC CACAAGTTTA AAGTCACCTT TTGAGGAAAA ATTCCTTTCT 
CAGCAGCCAC AAACTTCAGA TAGGTGGCCA AATTTTCTGT GTGTTTGCTC CGTCTTCTCA 
TCAGGCTCCA TTCAACTTCA CTGGTCTCAG TGGCCTCCTC AGAATGGTGC AACATCAAAG 
TGGTTTTCCA CAGCCAAAGG ACTATTGGGA GCTGGACCTA GTGGAATTAT GGCTGCTGAT 
GCTATCATAA CAGACACTGG TGCCATGCAT GTTGCAGGTG TTCCGATTGT AAATCCATCG 
ACTGTTGTTG TTTGGGAGGT CACACCAGGC CCTGGAAATG GTTTTCAAGT GATCCCAAAG 
TCAAGTGCGA CCAATGGGGT CCCACCTTCT CTCAATCCTC CTAGTTGGGC AGGTTTTTCC 
CCTTTGGCTG CATATTTATT TAGCTGGCAA GAGTTTTTGG CTTCTGAAAT GAAGCAAGGG 
AGGAAGCAGA CAGATCAAGA TTTCAATGAC GCTGTATTAC TACATTGTTC ACCAGTTTCA 
AATTTTTCTG CATATGTGAG TCCTGAGACT GCAGCTCAAT CAGCAGCAAC TACCACATGG 
GGTTCTGGAG TCACTGCAGT TGCCTTTGAT CCAACTCGTG GAGGTTCAGT GATAGCTGTT 
GCGATAGTTG AAGGGCAGTA CATGTCTCCC TATGATCCAG ATGAGGGCCC TTCAATCACA 
GGATGGAGGG TGCAACGCTG GGAATCATCT CTTCAGCCTG TTGTCCTCCA TCAAATATTT 
GGAAATCCCA CTTCCAGTTT TGGGGGGCAG GCACCTGTGC AAACAGTTTG GCAAACAAAA 
GTCAATAAAA GCATCCCACC AACTAGTGAT TTTAAAAGTC AACAAACACC TGCAACAGGA 
CCAACCTCTG AGAGAAATAC ATCTGATTCT TCTATGGATA TGGGCAAAAG AGTCAGCTTT 
GATCCCTTTG ATTTGCCTAG TGATGTTAGA ACACTTGCTC AAATTTCATA TTCTGCTCAT 
GGTGGTGAGA TTGCTGTTGC TTTTCTAAGG GGTGGGGTGC ACATCTTTTC TGGTCCAAAC 
TTTACACCTG TTGATAACTA CCAGATTAAC GTTGGCTCTG CAATTGCTAC TCCTGCATTC 
TCATCTACAA GCTGCTGTTC AGCTTCTGTT TGGCATGATA CCAACAAGGA CAGAACTATG 
TTGAAGATAA TTCGCGTTCT CCCCCCTGCT GTTCCAAGTA GTCAAGTAAA AGCCAACTCA 
TCAAACTGGG AACGTGCAAT TGCTGAGAGG TTTTGGTGGA GCCTTTTGGT TGGAGTTGAT 
TGGTGGGATG CTGTTGGATG TACACAGAGC GCAGCTGAGG ATGGCATTGT TTCTTTGAAC 
AGTGTCATTG CAGTGTTGGA TGCAGATTTC CATTCTCTCC CTTCTACTCA GCACCGACAA 
CAGTATGGCC CTAGTCTGGA CAGGATAAAA TGCAGACTAT TGGAAGGTAT AAACGCGCAG 
GAGGTTAGGG CAATGGTTCT GGATATGCAA GCAAGGTTAC TGCTGGATAT GCTGGGGAAA 
GGAATTGAAT CAGCGCTAAT GAAACCTTCA GCTCTAGTAC CTGAGCCATG GCAAGCAACT 
GGTGAGACAT TATCTGGCAT TGATCCAGAA GAAATGGCCG TTGACCCAGC ACTAGTTTTT 
AGCATTCAGG CTTATGTTGA TTCCGTCCTT GATCTAGCTT CACACTTCAT CACTCGCTTG 
AGGCGTTATG CAAGTTTCTG TCGTACTTTG GCAAGTCATG CTGTTACTGC AGGCACGGGC 
AGCAATCGAA GTGCAGTGGC TAGTCCTACC CAAAGTTCTG CATCTCCTGC AACAAGTCAG 
GGAGGTCAAA GTGGTACCAC CAGTTCCACA GGGAGCACCC AGATGCAAGC TTGGGTACAA 
GGTGCTATTG CTAAGATTAG TAGCACCACC GATGGAGTTC CAAATTCGAC CCCAAATCCC 
ATAAATGGTC CACCATCCTT CATACCCATA AGTATTAATA CTGGGACATT TCCTGGAACT 
CCAGCTGTTA GGCTTATTGG GGACTGCCAT TTCCTTCATA GATTATGCCA ACTTTTGCTT 
TTTTGTTTTT TCTTCCGACG AACACAATTA CCTCGTGTTA TTGGGGTTGC ACAGAGGAAT 
GCTGATACCA ATATGCATAA ACCCCAAGCT ACTGGGACCA GCAAGGTGGA GGAGAACAGT 
ACTGTTTCTA ACAAACTGGC ATCAGCTATG GCAAGATCGG AAGAGGGTCA GGTTGCTCGA 
GCTAATCATG CCATCAATGG AGCTAAAGGA GCTGAAGAAG GTCCTACAGG CCGGTCAAGA 
TTAGGTTCTG GAAATGCTGG GCAAGGATAC ACATTTGAGG AGGTGAAGGT CCTTTTCCTC 
ATACTTATGG ATCTCTGTCG TAGAACAGCC GCTCTGCAAC ATCCATTGCC AATTTCTCAG 
GTGGGAAGCA ACAACATCCA GGTCCGTCTG CATTACATAG ATGGGAGTTA TACTGTCTTA 
CCAGAGGTTG TAGAAGCATC TCTTGGTCCA CATATGCAGA ATATGCCCCG GCCTAGAGGT 
GCAGATGCTG CTGGTCTTTT ACTTCGTGAG TTAGAGCTCC ATCCTCCTGC TGAAGAGTGG 
CATAGGCGGA ATATGTTTGG TGGGCCCTGG TCTGATCCTG AGGATATGGG TTCTGTAGAT 
GATACTCCCA AACTAAGTAC CTATGCTGAT CCACTTGAAT CAAACTCATT TGAAAATAAT 
GATACATATT ATGGAGCCCG TGGTTTGTGG CCAAGGAAAC GCAGGTTATC TGAAAGAGAT 
GCAGCTTTTG GCTTGAACAC TTCGGTGGGA TTGGGTGCAT ATCTTGGAAT AATGGGATCT 
AGAAGAGATG TTGTCACTGC TGTCTGGAAG ACTGGTCTGG AAGGGGTTTG GTACAAGTGC 
ATAAGATGTT TGCGGCAGAC TTCAGCTTTT GCAGCAGATG CTACCAATCA ACCTAATCAG 
AATGAGCGGG AGATGTGGTG GATCAGCCGT TGGGCTTATG GCTGCCCAAT GTGTGGTGGA 
ACATGGGTTC GGGTTGTATA G