WNK1 cDNA ORF clone, Nipponia nippon(crested ibis)

Gene
Clones
gRNAs

Summary

Gene Symbol	WNK1
Entrez Gene ID	104020216
Full Name	WNK lysine deficient protein kinase 1
Gene Type	protein-coding
Organism	Nipponia nippon(crested ibis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009473602.1	XP_009471877.1	serine/threonine-protein kinase WNK1

Pathways from BioSystems
Gene Ontology

The following WNK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONi173391	XM_009473602.1 Latest version!	Nipponia nippon WNK lysine deficient protein kinase 1 (WNK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WNK1 ( XM_009473602.1 ) cDNA ORF clone, Nipponia nippon(crested ibis) -> XP_009471877.1 Nipponia nippon WNK lysine deficient protein kinase 1 (WNK1), mRNA.

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CloneID	ONi173391
Clone ID Related Accession (Same CDS sequence)	XM_009473602.1
Accession Version	XM_009473602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-05
Organism	Nipponia nippon(crested ibis)
Product	serine/threonine-protein kinase WNK1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009002850.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nipponia nippon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGG ACAGCCGAGG GGCGGCAGCC ACCGAGCACC GCTTCTTCCG CCGTAGCGTG 
ATCTGCGACT CCAACGCTAC GGCCTTGGAG CTACCCAGTT TGCAGCCCGC AGCGCCCTCG 
GCCCCTGTCT CGGGAGGCAG CGCAGCTCCG TTGGTCTCTC CTCCCGAGTG TGCCAGCCGG 
ACCTCTTGCA TCGCTGTCAG CGCCACCCAG GCTCCCTCGC TGCTGCAGCA GCCACCGCCG 
CCTGCCCCGC TTCCCCTCGA AGGACAGCCT GCTCAGGAGC CCCCTGCCAC AAAGGATGCC 
GCCCCGCTGC TGCCCAAGGA GGAGGAGGAT GAGGCAACCG CGCTACCCGC CACCAGTGCC 
GCTGGCAGCG TGTCAGCTGC CAGCCGGGAA TTTGAGGAGC GGAGAACACA GCAGGAAGAC 
ATAGAAGAGC TTGAGACCAA GGCAGTGGGC ATCTCCCCGG ATGGCCGCTT CCTGAAGTTT 
GACATCGAGA TTGGAAGAGG CTCTTTCAAA ACTGTGTATA AAGGACTGGA TACGGATACC 
ACTGTGGAAG TCGCCTGGTG CGAGCTGCAG GATCGGAAGC TGTCCAAGTC AGAGCGACAA 
AGATTTAAAG AGGAAGCTGG GATGCTGAAA GGGCTTCAGC ATCCCAATAT TGTCAGGTTC 
TATGATTCCT GGGAGTCTAC AGTCAAAGGA AAAAAGTGTA TTGTTCTTGT GACAGAACTC 
ATGACATCTG GAACACTGAA AACGTATTTG AAAAGATTTA AAGTGATGAA AATTAAAGTA 
CTGCGAAGCT GGTGCCGTCA GATACTGAAA GGCTTACAAT TTCTACACAC GCGCACTCCT 
CCCATTATTC ATAGAGACTT AAAATGTGAC AACATCTTCA TTACTGGCCC CACTGGATCA 
GTCAAGATTG GAGACCTTGG TCTGGCTACC CTGAAACGAG CTTCTTTTGC CAAAAGTGTG 
ATAGGTACCC CAGAGTTCAT GGCCCCCGAG ATGTATGAGG AGAAATATGA TGAATCCGTT 
GATGTATATG CTTTTGGGAT GTGTATGCTA GAGATGGCCA CGTCTGAGTA TCCTTATTCC 
GAGTGCCAAA ATGCTGCGCA GATCTACCGT CGAGTGACCA GTGGTGTCAA GCCAGCCAGC 
TTCGATAAAG TAGCGATCCC TGAAGTGAAG GAGATTATTG AGGGATGCAT TCGGCAAAAC 
AAAGGCGAAA GATATGCTAT TAAGGACCTT TTGAATCATG CTTTCTTCCA AGAAGAGACT 
GGTGTACGGG TAGAACTAGC AGAAGAAGAT GATGGAGAAA AAATTGCCAT TAAACTCTGG 
CTACGAATTG AAGACATCAA AAAACTCAAG GGCAAATATA AAGATAATGA GGCAATAGAG 
TTTTCCTTTG ATTTGGAGAG AGATGTCCCA GAGGATGTAG CACAGGAAAT GGTGGAATCT 
GGCTATGTCT GTGAAGGGGA TCACAAGACA ATGGCGAAAG CTATCAAGGA CAGGGTATCT 
TTGATTAAGC GAAAGCGAGA GCAGCGTCAG TTGGTGCGAG AAGAACAGGA GAAGAAGCTT 
CAGGAAGAAG GTAGTCAGAA GCAGCAGCTG GAGCAACAGC AACCATCAAG TGCTTCCCAT 
GCAGGGTCAA AGCATCCCCT ATCTGTCACT GGTACTACTC CTGTCCCCAC TACTTCAGCA 
TCGGTTTCTA CACAAGTGGA GCCTGAAGAA CCAGAAGCTG ATCAACACCA ACAACTGCAG 
TTCCAGCAAC CCAGTATATC TATTCTTTCT GATGGGACAG TTGACAGTGG CCAGGGGTCA 
TCTGTTTATA CCGAATCATG TGTGAGCAGT CAACAGACTG TGTCGTATGG TTCCCAGCAT 
GACCAGTCAA TCTCAACAGC TGCAGTCCAG GGATATGCAG CTTCAGTTGG CCAAGTGCAG 
TCGCAGCAAC ATGGAGGATA TCAGCCACCT GCAGCGCCTC AACGTGGTCG TAGCATGTCA 
GTTTGTGTCC CCCACCTTCC TGCTCTTCCC TCTATGTCCT GCATCCCTCT CAGTGCTCCT 
TGCACCCCAC CACCAGCGCT GTCTGCACCT CTTTCTCCTT TCTACCTTCG GACTGTCCCT 
GAAGAAACCT TTGCAGAAAA GCTTTCTAAA GCCCTGGAAA GTGTCCTGCC CATGCACTCT 
GCCTCTCCAC GCAAGCATCG ACGCTCCAGC CTGCCCTCCC TCTCTGTCAG TCCTCCTCAG 
CATCCGCGTG GGGGAACACC AACGTTCCCA GATTCACAGA TATTTTTCCC AACCGTTCAT 
GAACGGCCGG TGTCTTTCTC ACCACCACCT GTCTGCCCGC CGAAGGTGGC AGTTGCTCAG 
CGGCGCAAGA GCACTTCATT TTTGGAAGCC CAGACTTGCC ACTTCCAGCC ATTGCTGAAG 
ACTGGGCAGA GTTTAGTTCC ACCTGGTGGT AGCCCCACCA ATTGGACACC AGAGGCATTC 
GTTATGCTGG GCACAGCAGC TCAGAGAGTC GCTGGAGAGC CTCTGCATGT GCAAGTTAAT 
CCCGTGTTTG AGCCAGCTCC TGTACACAGT GACTATAGAT CTGGACCAAC ACCTCCAGAG 
GATGCTCACT ACAGAATGCA TCCAGAAGCT GTATATTTGG TGGGCATGCA CTATCCATCA 
CAGACACAGG TGCAAGGCCA GTCAGCCTCA AGCAGTGCAT CAGTGCCATC CCAGCCTACC 
CAACACACTC AACAGAATGC ACAACAGCCA GCCCCTTCCC AACAGCCTGG ACAGTACCAG 
CTGCAGCAGC CATCAGTTTC TGCTGGTGCC ACTCCCACAC AAACTGTCTC TCAAACTCAA 
GCTTCACAGA TTATGCCAAT GCCTCAGGCA GCAGCTGGGA CACAGCTTCC TGTTTCCCAA 
CCAGTGTCGA TCATCCAAGG CGAGCCTCAA TTACCTGTTG CAGCACCTTC TCTCCCTCAG 
CCTTCAGTTG CCCCATCAGT CCCCATTGGC TCTCATTTTC TACCCATGGG ACAGCCCTTG 
CCAACTTCCA TGGTTCCCCA GTTCTCAGTC TCTCAGTTGC CCATAGCAGC TCCTCATGTG 
TCAGTGGCTC AGCCAGGTTT CCAGTCACTG CCCATTTCAA TGGCAGCTGG CATGAATCAG 
CCATTGCTCA CACTGGCAAC GTCTGCTGCG GCAACCGCTG TCCCTGTAGG ATCAACCGTT 
GTCCCTAGCC AGCTTCCCAC ACTGATGCAG CCTGTGGCTC AGTTGCCCAG CCAGGTTCTC 
CCGCAGCTCC TGCAGCCGGC TGTCCAGTCC GTGGGATTGC CAGTCAGCAT TGGACAAGCT 
GCTGAAGCTT CACTTCCTGC TGGAGATGCT CTTTACCAGG GCTTCCCGTC GCGGCTGCCG 
CCTCAATACC CAGGCGACTC GAATGCTGCA CCTTCCTCTG CTGTGGCCTC TGTTAGCATT 
CCTTCTGCCG TACTGTCCCC TCCCCTACCC ACAGATGCAA TGGCTCAGTC GGGCTACCTT 
GCTCCTGTTG TGCAGCCGTA TGTGGAGCAG AATGTTTTAG TTCCCATGGG TGGCGTTGGA 
GGACAGGTTC AAGTGCCCCA GCCTACAGTG AGTTTAGCAC AACAGGCCTC ATCTGCCTCC 
TCGCAGCAGG CAGTTTTGGA GGGTACTCAA GGAGTCTCAC AGACTGCTCC TTCAGAGGCT 
CTTCCAGCAA CACAGCCTGC TCAGTCTACC CCTTTGGCTT CCTCTATGGA TAGTGCGCAT 
TCTGATGTTG CTTCAGGATT GAGTGATGGC AACGAGAATG TTCCAGCTTC TAGTGGAAGG 
CATGAAGGGA GGACTACTAA ACGTCATATG CGGAGGTCTG TCCGCAGCCG CTCTCGCCAT 
GAGAAGACTG CTAGGCCGAA ACTGAGAATT CTAAACGTTT CAAATAAAGG TGATCGGGTA 
GTGGAATGCC AGCTAGAGAC ACACAATCGA AAAATGGTTA CTTTCAAATT TGATTTGGAT 
GGTGACAACC CTGAGGAAAT AGCTTCAATT ATGGTGCAGA ATGAGTTTAT TTTAGCAACT 
GAGAGAGACT CATTCGTAGA CCAGGTACGA GAGATTATTG AGAAAGCAGA TGAAATGCTG 
AGTGAGGATG TAAGTGTGGA GCCAGAAGGT GATCAGGGTT TGGAGAGTAT GCGGACAAAA 
GATGATGGCT TCTTTCCAGG GTCGCAGAAA TTAGAGTTCA AACAGCCAGA TCCTACATCT 
TCCATGCCAC AAAGAATAGG GGTTCCTCCT AGCTCCTTTA CCCAGGTTGT CCATTCTGCA 
GGAAGACGGT TTATTGTCAG CCCTGTCCCT GAGAGTCGGT TAAAAGAACA GGGCTTTTTC 
ACATCTGCTG TTCCTGGAGG AAAAGAAACC CCAGATATGG TTGCTGCATC TCCACTACAT 
GGTCCTGGAA TGAATCTCTC CCACTCGGCA TCATCACTGA GTTTGCAGCA AGCTTTCTCT 
GAGATGGGGC ATGCTCAAAT GACAGAAGGA CCTAGTACAG CCCCTCCAGT GTTCAATCAA 
ACAATACCAC CATTTCCACC TGCACTTTCT ACTATGGCAG GCAGTGGTGC ACCTTCTGCA 
TCTGTGGCTG CTGCTTCAAT ATCTCTTCCC TCCAGCACAG GTGCTTCTCT TCCAGGTTCT 
GTTACTTTGC CTTCAGAAAG TGCAGCTGTT GGAGTTGCAC CAAGTGCAAG CGTGCCAAGC 
TCTATTTCTC CACCTCCAGC CTCACAATCT GGACAGCCAT CGACTGGTGT CGCTTCCAGT 
GTGAGCGCCC CTGCTTCTTT CTCCTTACCT ACCACATCCC AGCCTGCTCA GCCAGTAACT 
GGAGACATCG CCCCCAGCAT CAGCACACCG TCTTCTTTGG CACTGCCATC TACCCAGGTT 
CCTGGTGTCA CTGGTCTTGG TGTCGTACCA GCTGTGACAT CTCAGTCTAC TCCTCAGATT 
GTTAGTAGTA TGACAGCACC ACAGACAACT GTTGCCCTGT CGCTGGCTCA GAATATGGCT 
TTGCAGCTTC CTCAGCTTAG TAGCAGTGGC TCTGTCTCCA ATCTGGCAGA AACAACAGTT 
GTAAGTGCTC CACAGTCACT ACCTGAGAGT GGGCAGTCTA TGGATAAATC ACAGCTCTGC 
AGTGCAGCTG GCTTATCTCT TCCGATCTCT GCACCTTTAT CGTCCTCAGT AGCAACAAGC 
ATATGTGGTT CAGTCACTCA GCCAGTGATA CATCCCTTAC TGGTCCCATC AGGAATTACA 
TCAACACCCG TCCTACCCCA GATTCCAGGT GCAACACCCA TGTTGCCCCA GGTTCCCTTA 
CCTGGTGTCT TACCACAACC AGTTACTAAC TTGCCTGCAG TACAGCAAAC TTTGATACAC 
AGCCAGCCTC AACCAGCTCC ATTACCCAAT CAGCCTCATA TTCACTGTCT GGAGGCTGAT 
GCTGATGCTC AGTCTAAAGC TCCTGGTATT GATGATATAA AGACCCTGGA AGAAAAGCTA 
CGGTCTCTCT TCAGTGAACA TGGTAATGTG GGAACAGCGC ATCCGTCAGT ATCTCTGGAG 
ACGTCATTGG TAATGGAAAC TACAGTTATT CCTGGGATAC CAACCACTGC TGTTGCACCA 
ACTAAACCCC TGACATCCAT TAGCAGTTGT ATACCCCCGA GCAGCTTGCC TCTTGGACCA 
ACTGGCTTGC CAGTGCTGAC ACCAGTTGCC ACTCCCGGGC AAGTGATCAC CCCAGTCAGC 
TATATTTCGG CGTCGAGCAG CATTGCAACA GCAGTAGTGA AACCAGGGAC CTCTCCTTCA 
AAGCCCCCTC TTAGCAGGGT ACCGGTGCTA CCAGTAGGTT CTGAACTTCC AGCTGGCACT 
CCATCCAGTG AGCCGCTGCC ACCTTTTCCA GGACCTTCAC TAACTCAGTC CCAGCAGCCA 
CTGGAAGATC TGGATGCTAA GCTGAGAAGA ACCCTTAGTC CAGAGACTGT TCCAGTGACA 
TCTGCTCCTG CCTGTTCTGT GCCCTCAGCA TCTACAATGG TTACTGGGCT GGTGTCAACA 
GCAACACAGT CTCTGAAGGA TGCTTCAGCA TCATGTGGTG GAGAAAGCAG TGCTATGGCT 
ACCACAGCAG GAGCTGGGGT TCTTAAGATG GGACGGTTTC AGGTATCCGT GGCAGTGGAT 
GATGTGCTAA AAGAGGGTGA CAAACCTGAA ACTAAGCTGG TGCAATTTGA AACCACCTCC 
ACTGATTCTT CATCTCTTTC TGGCAGTAGC CCAGAGAGTA CACTGGTAAA GCAGGCTGGC 
AGTCGGAAAA GTGAGGCTGT CGCTGACTCT TCCTTGGATG TGGTAGATGG CATTCCTCAG 
GCAGTTCCTG TGCTGCAATT ACCAGTGGAT GTTGGTCATC CTACTAAGGT CGGACGCTTT 
CAGGTAACAA CAACAACGGA TCAAGTGGGG CGCTTCTCGG TGTCAAAGAC TCAGGATGAG 
GTCAGCTGTG CAGAGAAAGA GCCAATGACT CTTCCTCTCT CTGTGGACTT GGAACAAGTT 
GCTTCTTCAG CTGCAGCTCC AAAGAAAGAG TTGGAGTCAA GGCAGTCCCC ACACATGAAT 
GGTCCATCCT CTGAACCGGA GGCCGCCTTC TTAAGCGGGA TGGCTAAGGA TTTGGATGAT 
GGCTCAGGGA GCCCAGACTC CCTCCAGCCC CTGGGGTCGA AGATCAGCCT CCCTGTTCAG 
AGCCTTAGCA ACTCATTCAA CTCTTCCTAC ATGAGCAGCG ACAATGAATC GGATATTGAA 
GATGAAGACT TGAAATTGGA ACTCCGTCGG TTACGGGAGA AGCACCTTAA AGAGATCCAG 
GAGCTGCAGA GTCGCCAGAA GCAGGAAATT GAGTCACTGT ACATGAAATT GGGAAAGGCC 
CCACCTGCAG TAATTATTCC ACCAGCTGCA CCCCTTTCAG GAAGGAGGAG ACGACCTACT 
AAAGGAAAAA GCAGCAAGTC CAGTCGTAGC AGCTCCCAGG GAAATAAGAG CCCTCAGCTA 
TCAGGCAATC TGTCAACTCA AAGTGCGCCA TCAGTCTTGC CCCCACAGCA GACTCTTCAT 
CCTCCTGGTA GTGTGCCAGA GACTGGGCAG AACCATTTGT TACAGCCCCT CAAACCTTCA 
CCTTCTAGCG AAAATCTCTA CTCTGCCTTT ACCAGTGATG GTGCCCTTTC GGTACCCAGC 
CTTTCGGCAC CAGGCCAAGG CTGTGCGAAG TTTAACTGTG CATCTGAGAG AGTGACGTTC 
AAGCCTGGTG GCAGGAGGAC CCGATTTCTG AGGAAGATGG TGAAAAAAGT CTGTCCTTGC 
AACCAGCTCT GTAGGACCAG CAGCACTAAC ACAGTTGGGG CTACGGTGAA CAGCCAAGCA 
CCCCAGTCTC AGCCTACTGC TATTGCCTCA AGCCGGAAAG GGACTTTCAC AGATGACCTG 
CACAAGCTGG TAGATAACTG GGCCCGAGAC GCCATGAACC TGTCTGGCAA GAAAGTTGGC 
AAAGGGCATA GCAACTATGA GGGCCCTGGA ATGGCAAGAA AATTCTCGGC ACCAGGTCAG 
CTCTGTATCT CTATGACATC TTCCCTGGGT GCTACGCCCA TCTCTGCAGC ATCAGCTACC 
TCTTTAGGTC CCTTCACAAA GGCTATGTGC CCTCCGCAGC AGTACGGTTA CCCAGCAGCC 
TCTTTTGCCG CTCCGTGGAG TGGGACGGGG GGCCCAGCGC AGCCGCCGCT CGGCCAGTTC 
CAGCCCGTAG GCGCCGCGTC TTTGCAAAGC TTCAACATCA GCAATTTGCA AAAATCGATC 
AGCAACCCTC CAGGCTCCAA CCTGCGGACC ACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009471877.1
CDS	1..7776
Translation	MDKDSRGAAATEHRFFRRSVICDSNATALELPSLQPAAPSAPVSGGSAAPLVSPPECASRTSCIAVSATQAPSLLQQPPPPAPLPLEGQPAQEPPATKDAAPLLPKEEEDEATALPATSAAGSVSAASREFEERRTQQEDIEELETKAVGISPDGRFLKFDIEIGRGSFKTVYKGLDTDTTVEVAWCELQDRKLSKSERQRFKEEAGMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKGERYAIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKLQEEGSQKQQLEQQQPSSASHAGSKHPLSVTGTTPVPTTSASVSTQVEPEEPEADQHQQLQFQQPSISILSDGTVDSGQGSSVYTESCVSSQQTVSYGSQHDQSISTAAVQGYAASVGQVQSQQHGGYQPPAAPQRGRSMSVCVPHLPALPSMSCIPLSAPCTPPPALSAPLSPFYLRTVPEETFAEKLSKALESVLPMHSASPRKHRRSSLPSLSVSPPQHPRGGTPTFPDSQIFFPTVHERPVSFSPPPVCPPKVAVAQRRKSTSFLEAQTCHFQPLLKTGQSLVPPGGSPTNWTPEAFVMLGTAAQRVAGEPLHVQVNPVFEPAPVHSDYRSGPTPPEDAHYRMHPEAVYLVGMHYPSQTQVQGQSASSSASVPSQPTQHTQQNAQQPAPSQQPGQYQLQQPSVSAGATPTQTVSQTQASQIMPMPQAAAGTQLPVSQPVSIIQGEPQLPVAAPSLPQPSVAPSVPIGSHFLPMGQPLPTSMVPQFSVSQLPIAAPHVSVAQPGFQSLPISMAAGMNQPLLTLATSAAATAVPVGSTVVPSQLPTLMQPVAQLPSQVLPQLLQPAVQSVGLPVSIGQAAEASLPAGDALYQGFPSRLPPQYPGDSNAAPSSAVASVSIPSAVLSPPLPTDAMAQSGYLAPVVQPYVEQNVLVPMGGVGGQVQVPQPTVSLAQQASSASSQQAVLEGTQGVSQTAPSEALPATQPAQSTPLASSMDSAHSDVASGLSDGNENVPASSGRHEGRTTKRHMRRSVRSRSRHEKTARPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIASIMVQNEFILATERDSFVDQVREIIEKADEMLSEDVSVEPEGDQGLESMRTKDDGFFPGSQKLEFKQPDPTSSMPQRIGVPPSSFTQVVHSAGRRFIVSPVPESRLKEQGFFTSAVPGGKETPDMVAASPLHGPGMNLSHSASSLSLQQAFSEMGHAQMTEGPSTAPPVFNQTIPPFPPALSTMAGSGAPSASVAAASISLPSSTGASLPGSVTLPSESAAVGVAPSASVPSSISPPPASQSGQPSTGVASSVSAPASFSLPTTSQPAQPVTGDIAPSISTPSSLALPSTQVPGVTGLGVVPAVTSQSTPQIVSSMTAPQTTVALSLAQNMALQLPQLSSSGSVSNLAETTVVSAPQSLPESGQSMDKSQLCSAAGLSLPISAPLSSSVATSICGSVTQPVIHPLLVPSGITSTPVLPQIPGATPMLPQVPLPGVLPQPVTNLPAVQQTLIHSQPQPAPLPNQPHIHCLEADADAQSKAPGIDDIKTLEEKLRSLFSEHGNVGTAHPSVSLETSLVMETTVIPGIPTTAVAPTKPLTSISSCIPPSSLPLGPTGLPVLTPVATPGQVITPVSYISASSSIATAVVKPGTSPSKPPLSRVPVLPVGSELPAGTPSSEPLPPFPGPSLTQSQQPLEDLDAKLRRTLSPETVPVTSAPACSVPSASTMVTGLVSTATQSLKDASASCGGESSAMATTAGAGVLKMGRFQVSVAVDDVLKEGDKPETKLVQFETTSTDSSSLSGSSPESTLVKQAGSRKSEAVADSSLDVVDGIPQAVPVLQLPVDVGHPTKVGRFQVTTTTDQVGRFSVSKTQDEVSCAEKEPMTLPLSVDLEQVASSAAAPKKELESRQSPHMNGPSSEPEAAFLSGMAKDLDDGSGSPDSLQPLGSKISLPVQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLREKHLKEIQELQSRQKQEIESLYMKLGKAPPAVIIPPAAPLSGRRRRPTKGKSSKSSRSSSQGNKSPQLSGNLSTQSAPSVLPPQQTLHPPGSVPETGQNHLLQPLKPSPSSENLYSAFTSDGALSVPSLSAPGQGCAKFNCASERVTFKPGGRRTRFLRKMVKKVCPCNQLCRTSSTNTVGATVNSQAPQSQPTAIASSRKGTFTDDLHKLVDNWARDAMNLSGKKVGKGHSNYEGPGMARKFSAPGQLCISMTSSLGATPISAASATSLGPFTKAMCPPQQYGYPAASFAAPWSGTGGPAQPPLGQFQPVGAASLQSFNISNLQKSISNPPGSNLRTT

Target ORF information:

RefSeq Version	XM_009473602.1
Organism	Nipponia nippon(crested ibis)
Definition	Nipponia nippon WNK lysine deficient protein kinase 1 (WNK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009473602.1

ORF Insert Sequence:

ATGGACAAGG ACAGCCGAGG GGCGGCAGCC ACCGAGCACC GCTTCTTCCG CCGTAGCGTG 
ATCTGCGACT CCAACGCTAC GGCCTTGGAG CTACCCAGTT TGCAGCCCGC AGCGCCCTCG 
GCCCCTGTCT CGGGAGGCAG CGCAGCTCCG TTGGTCTCTC CTCCCGAGTG TGCCAGCCGG 
ACCTCTTGCA TCGCTGTCAG CGCCACCCAG GCTCCCTCGC TGCTGCAGCA GCCACCGCCG 
CCTGCCCCGC TTCCCCTCGA AGGACAGCCT GCTCAGGAGC CCCCTGCCAC AAAGGATGCC 
GCCCCGCTGC TGCCCAAGGA GGAGGAGGAT GAGGCAACCG CGCTACCCGC CACCAGTGCC 
GCTGGCAGCG TGTCAGCTGC CAGCCGGGAA TTTGAGGAGC GGAGAACACA GCAGGAAGAC 
ATAGAAGAGC TTGAGACCAA GGCAGTGGGC ATCTCCCCGG ATGGCCGCTT CCTGAAGTTT 
GACATCGAGA TTGGAAGAGG CTCTTTCAAA ACTGTGTATA AAGGACTGGA TACGGATACC 
ACTGTGGAAG TCGCCTGGTG CGAGCTGCAG GATCGGAAGC TGTCCAAGTC AGAGCGACAA 
AGATTTAAAG AGGAAGCTGG GATGCTGAAA GGGCTTCAGC ATCCCAATAT TGTCAGGTTC 
TATGATTCCT GGGAGTCTAC AGTCAAAGGA AAAAAGTGTA TTGTTCTTGT GACAGAACTC 
ATGACATCTG GAACACTGAA AACGTATTTG AAAAGATTTA AAGTGATGAA AATTAAAGTA 
CTGCGAAGCT GGTGCCGTCA GATACTGAAA GGCTTACAAT TTCTACACAC GCGCACTCCT 
CCCATTATTC ATAGAGACTT AAAATGTGAC AACATCTTCA TTACTGGCCC CACTGGATCA 
GTCAAGATTG GAGACCTTGG TCTGGCTACC CTGAAACGAG CTTCTTTTGC CAAAAGTGTG 
ATAGGTACCC CAGAGTTCAT GGCCCCCGAG ATGTATGAGG AGAAATATGA TGAATCCGTT 
GATGTATATG CTTTTGGGAT GTGTATGCTA GAGATGGCCA CGTCTGAGTA TCCTTATTCC 
GAGTGCCAAA ATGCTGCGCA GATCTACCGT CGAGTGACCA GTGGTGTCAA GCCAGCCAGC 
TTCGATAAAG TAGCGATCCC TGAAGTGAAG GAGATTATTG AGGGATGCAT TCGGCAAAAC 
AAAGGCGAAA GATATGCTAT TAAGGACCTT TTGAATCATG CTTTCTTCCA AGAAGAGACT 
GGTGTACGGG TAGAACTAGC AGAAGAAGAT GATGGAGAAA AAATTGCCAT TAAACTCTGG 
CTACGAATTG AAGACATCAA AAAACTCAAG GGCAAATATA AAGATAATGA GGCAATAGAG 
TTTTCCTTTG ATTTGGAGAG AGATGTCCCA GAGGATGTAG CACAGGAAAT GGTGGAATCT 
GGCTATGTCT GTGAAGGGGA TCACAAGACA ATGGCGAAAG CTATCAAGGA CAGGGTATCT 
TTGATTAAGC GAAAGCGAGA GCAGCGTCAG TTGGTGCGAG AAGAACAGGA GAAGAAGCTT 
CAGGAAGAAG GTAGTCAGAA GCAGCAGCTG GAGCAACAGC AACCATCAAG TGCTTCCCAT 
GCAGGGTCAA AGCATCCCCT ATCTGTCACT GGTACTACTC CTGTCCCCAC TACTTCAGCA 
TCGGTTTCTA CACAAGTGGA GCCTGAAGAA CCAGAAGCTG ATCAACACCA ACAACTGCAG 
TTCCAGCAAC CCAGTATATC TATTCTTTCT GATGGGACAG TTGACAGTGG CCAGGGGTCA 
TCTGTTTATA CCGAATCATG TGTGAGCAGT CAACAGACTG TGTCGTATGG TTCCCAGCAT 
GACCAGTCAA TCTCAACAGC TGCAGTCCAG GGATATGCAG CTTCAGTTGG CCAAGTGCAG 
TCGCAGCAAC ATGGAGGATA TCAGCCACCT GCAGCGCCTC AACGTGGTCG TAGCATGTCA 
GTTTGTGTCC CCCACCTTCC TGCTCTTCCC TCTATGTCCT GCATCCCTCT CAGTGCTCCT 
TGCACCCCAC CACCAGCGCT GTCTGCACCT CTTTCTCCTT TCTACCTTCG GACTGTCCCT 
GAAGAAACCT TTGCAGAAAA GCTTTCTAAA GCCCTGGAAA GTGTCCTGCC CATGCACTCT 
GCCTCTCCAC GCAAGCATCG ACGCTCCAGC CTGCCCTCCC TCTCTGTCAG TCCTCCTCAG 
CATCCGCGTG GGGGAACACC AACGTTCCCA GATTCACAGA TATTTTTCCC AACCGTTCAT 
GAACGGCCGG TGTCTTTCTC ACCACCACCT GTCTGCCCGC CGAAGGTGGC AGTTGCTCAG 
CGGCGCAAGA GCACTTCATT TTTGGAAGCC CAGACTTGCC ACTTCCAGCC ATTGCTGAAG 
ACTGGGCAGA GTTTAGTTCC ACCTGGTGGT AGCCCCACCA ATTGGACACC AGAGGCATTC 
GTTATGCTGG GCACAGCAGC TCAGAGAGTC GCTGGAGAGC CTCTGCATGT GCAAGTTAAT 
CCCGTGTTTG AGCCAGCTCC TGTACACAGT GACTATAGAT CTGGACCAAC ACCTCCAGAG 
GATGCTCACT ACAGAATGCA TCCAGAAGCT GTATATTTGG TGGGCATGCA CTATCCATCA 
CAGACACAGG TGCAAGGCCA GTCAGCCTCA AGCAGTGCAT CAGTGCCATC CCAGCCTACC 
CAACACACTC AACAGAATGC ACAACAGCCA GCCCCTTCCC AACAGCCTGG ACAGTACCAG 
CTGCAGCAGC CATCAGTTTC TGCTGGTGCC ACTCCCACAC AAACTGTCTC TCAAACTCAA 
GCTTCACAGA TTATGCCAAT GCCTCAGGCA GCAGCTGGGA CACAGCTTCC TGTTTCCCAA 
CCAGTGTCGA TCATCCAAGG CGAGCCTCAA TTACCTGTTG CAGCACCTTC TCTCCCTCAG 
CCTTCAGTTG CCCCATCAGT CCCCATTGGC TCTCATTTTC TACCCATGGG ACAGCCCTTG 
CCAACTTCCA TGGTTCCCCA GTTCTCAGTC TCTCAGTTGC CCATAGCAGC TCCTCATGTG 
TCAGTGGCTC AGCCAGGTTT CCAGTCACTG CCCATTTCAA TGGCAGCTGG CATGAATCAG 
CCATTGCTCA CACTGGCAAC GTCTGCTGCG GCAACCGCTG TCCCTGTAGG ATCAACCGTT 
GTCCCTAGCC AGCTTCCCAC ACTGATGCAG CCTGTGGCTC AGTTGCCCAG CCAGGTTCTC 
CCGCAGCTCC TGCAGCCGGC TGTCCAGTCC GTGGGATTGC CAGTCAGCAT TGGACAAGCT 
GCTGAAGCTT CACTTCCTGC TGGAGATGCT CTTTACCAGG GCTTCCCGTC GCGGCTGCCG 
CCTCAATACC CAGGCGACTC GAATGCTGCA CCTTCCTCTG CTGTGGCCTC TGTTAGCATT 
CCTTCTGCCG TACTGTCCCC TCCCCTACCC ACAGATGCAA TGGCTCAGTC GGGCTACCTT 
GCTCCTGTTG TGCAGCCGTA TGTGGAGCAG AATGTTTTAG TTCCCATGGG TGGCGTTGGA 
GGACAGGTTC AAGTGCCCCA GCCTACAGTG AGTTTAGCAC AACAGGCCTC ATCTGCCTCC 
TCGCAGCAGG CAGTTTTGGA GGGTACTCAA GGAGTCTCAC AGACTGCTCC TTCAGAGGCT 
CTTCCAGCAA CACAGCCTGC TCAGTCTACC CCTTTGGCTT CCTCTATGGA TAGTGCGCAT 
TCTGATGTTG CTTCAGGATT GAGTGATGGC AACGAGAATG TTCCAGCTTC TAGTGGAAGG 
CATGAAGGGA GGACTACTAA ACGTCATATG CGGAGGTCTG TCCGCAGCCG CTCTCGCCAT 
GAGAAGACTG CTAGGCCGAA ACTGAGAATT CTAAACGTTT CAAATAAAGG TGATCGGGTA 
GTGGAATGCC AGCTAGAGAC ACACAATCGA AAAATGGTTA CTTTCAAATT TGATTTGGAT 
GGTGACAACC CTGAGGAAAT AGCTTCAATT ATGGTGCAGA ATGAGTTTAT TTTAGCAACT 
GAGAGAGACT CATTCGTAGA CCAGGTACGA GAGATTATTG AGAAAGCAGA TGAAATGCTG 
AGTGAGGATG TAAGTGTGGA GCCAGAAGGT GATCAGGGTT TGGAGAGTAT GCGGACAAAA 
GATGATGGCT TCTTTCCAGG GTCGCAGAAA TTAGAGTTCA AACAGCCAGA TCCTACATCT 
TCCATGCCAC AAAGAATAGG GGTTCCTCCT AGCTCCTTTA CCCAGGTTGT CCATTCTGCA 
GGAAGACGGT TTATTGTCAG CCCTGTCCCT GAGAGTCGGT TAAAAGAACA GGGCTTTTTC 
ACATCTGCTG TTCCTGGAGG AAAAGAAACC CCAGATATGG TTGCTGCATC TCCACTACAT 
GGTCCTGGAA TGAATCTCTC CCACTCGGCA TCATCACTGA GTTTGCAGCA AGCTTTCTCT 
GAGATGGGGC ATGCTCAAAT GACAGAAGGA CCTAGTACAG CCCCTCCAGT GTTCAATCAA 
ACAATACCAC CATTTCCACC TGCACTTTCT ACTATGGCAG GCAGTGGTGC ACCTTCTGCA 
TCTGTGGCTG CTGCTTCAAT ATCTCTTCCC TCCAGCACAG GTGCTTCTCT TCCAGGTTCT 
GTTACTTTGC CTTCAGAAAG TGCAGCTGTT GGAGTTGCAC CAAGTGCAAG CGTGCCAAGC 
TCTATTTCTC CACCTCCAGC CTCACAATCT GGACAGCCAT CGACTGGTGT CGCTTCCAGT 
GTGAGCGCCC CTGCTTCTTT CTCCTTACCT ACCACATCCC AGCCTGCTCA GCCAGTAACT 
GGAGACATCG CCCCCAGCAT CAGCACACCG TCTTCTTTGG CACTGCCATC TACCCAGGTT 
CCTGGTGTCA CTGGTCTTGG TGTCGTACCA GCTGTGACAT CTCAGTCTAC TCCTCAGATT 
GTTAGTAGTA TGACAGCACC ACAGACAACT GTTGCCCTGT CGCTGGCTCA GAATATGGCT 
TTGCAGCTTC CTCAGCTTAG TAGCAGTGGC TCTGTCTCCA ATCTGGCAGA AACAACAGTT 
GTAAGTGCTC CACAGTCACT ACCTGAGAGT GGGCAGTCTA TGGATAAATC ACAGCTCTGC 
AGTGCAGCTG GCTTATCTCT TCCGATCTCT GCACCTTTAT CGTCCTCAGT AGCAACAAGC 
ATATGTGGTT CAGTCACTCA GCCAGTGATA CATCCCTTAC TGGTCCCATC AGGAATTACA 
TCAACACCCG TCCTACCCCA GATTCCAGGT GCAACACCCA TGTTGCCCCA GGTTCCCTTA 
CCTGGTGTCT TACCACAACC AGTTACTAAC TTGCCTGCAG TACAGCAAAC TTTGATACAC 
AGCCAGCCTC AACCAGCTCC ATTACCCAAT CAGCCTCATA TTCACTGTCT GGAGGCTGAT 
GCTGATGCTC AGTCTAAAGC TCCTGGTATT GATGATATAA AGACCCTGGA AGAAAAGCTA 
CGGTCTCTCT TCAGTGAACA TGGTAATGTG GGAACAGCGC ATCCGTCAGT ATCTCTGGAG 
ACGTCATTGG TAATGGAAAC TACAGTTATT CCTGGGATAC CAACCACTGC TGTTGCACCA 
ACTAAACCCC TGACATCCAT TAGCAGTTGT ATACCCCCGA GCAGCTTGCC TCTTGGACCA 
ACTGGCTTGC CAGTGCTGAC ACCAGTTGCC ACTCCCGGGC AAGTGATCAC CCCAGTCAGC 
TATATTTCGG CGTCGAGCAG CATTGCAACA GCAGTAGTGA AACCAGGGAC CTCTCCTTCA 
AAGCCCCCTC TTAGCAGGGT ACCGGTGCTA CCAGTAGGTT CTGAACTTCC AGCTGGCACT 
CCATCCAGTG AGCCGCTGCC ACCTTTTCCA GGACCTTCAC TAACTCAGTC CCAGCAGCCA 
CTGGAAGATC TGGATGCTAA GCTGAGAAGA ACCCTTAGTC CAGAGACTGT TCCAGTGACA 
TCTGCTCCTG CCTGTTCTGT GCCCTCAGCA TCTACAATGG TTACTGGGCT GGTGTCAACA 
GCAACACAGT CTCTGAAGGA TGCTTCAGCA TCATGTGGTG GAGAAAGCAG TGCTATGGCT 
ACCACAGCAG GAGCTGGGGT TCTTAAGATG GGACGGTTTC AGGTATCCGT GGCAGTGGAT 
GATGTGCTAA AAGAGGGTGA CAAACCTGAA ACTAAGCTGG TGCAATTTGA AACCACCTCC 
ACTGATTCTT CATCTCTTTC TGGCAGTAGC CCAGAGAGTA CACTGGTAAA GCAGGCTGGC 
AGTCGGAAAA GTGAGGCTGT CGCTGACTCT TCCTTGGATG TGGTAGATGG CATTCCTCAG 
GCAGTTCCTG TGCTGCAATT ACCAGTGGAT GTTGGTCATC CTACTAAGGT CGGACGCTTT 
CAGGTAACAA CAACAACGGA TCAAGTGGGG CGCTTCTCGG TGTCAAAGAC TCAGGATGAG 
GTCAGCTGTG CAGAGAAAGA GCCAATGACT CTTCCTCTCT CTGTGGACTT GGAACAAGTT 
GCTTCTTCAG CTGCAGCTCC AAAGAAAGAG TTGGAGTCAA GGCAGTCCCC ACACATGAAT 
GGTCCATCCT CTGAACCGGA GGCCGCCTTC TTAAGCGGGA TGGCTAAGGA TTTGGATGAT 
GGCTCAGGGA GCCCAGACTC CCTCCAGCCC CTGGGGTCGA AGATCAGCCT CCCTGTTCAG 
AGCCTTAGCA ACTCATTCAA CTCTTCCTAC ATGAGCAGCG ACAATGAATC GGATATTGAA 
GATGAAGACT TGAAATTGGA ACTCCGTCGG TTACGGGAGA AGCACCTTAA AGAGATCCAG 
GAGCTGCAGA GTCGCCAGAA GCAGGAAATT GAGTCACTGT ACATGAAATT GGGAAAGGCC 
CCACCTGCAG TAATTATTCC ACCAGCTGCA CCCCTTTCAG GAAGGAGGAG ACGACCTACT 
AAAGGAAAAA GCAGCAAGTC CAGTCGTAGC AGCTCCCAGG GAAATAAGAG CCCTCAGCTA 
TCAGGCAATC TGTCAACTCA AAGTGCGCCA TCAGTCTTGC CCCCACAGCA GACTCTTCAT 
CCTCCTGGTA GTGTGCCAGA GACTGGGCAG AACCATTTGT TACAGCCCCT CAAACCTTCA 
CCTTCTAGCG AAAATCTCTA CTCTGCCTTT ACCAGTGATG GTGCCCTTTC GGTACCCAGC 
CTTTCGGCAC CAGGCCAAGG CTGTGCGAAG TTTAACTGTG CATCTGAGAG AGTGACGTTC 
AAGCCTGGTG GCAGGAGGAC CCGATTTCTG AGGAAGATGG TGAAAAAAGT CTGTCCTTGC 
AACCAGCTCT GTAGGACCAG CAGCACTAAC ACAGTTGGGG CTACGGTGAA CAGCCAAGCA 
CCCCAGTCTC AGCCTACTGC TATTGCCTCA AGCCGGAAAG GGACTTTCAC AGATGACCTG 
CACAAGCTGG TAGATAACTG GGCCCGAGAC GCCATGAACC TGTCTGGCAA GAAAGTTGGC 
AAAGGGCATA GCAACTATGA GGGCCCTGGA ATGGCAAGAA AATTCTCGGC ACCAGGTCAG 
CTCTGTATCT CTATGACATC TTCCCTGGGT GCTACGCCCA TCTCTGCAGC ATCAGCTACC 
TCTTTAGGTC CCTTCACAAA GGCTATGTGC CCTCCGCAGC AGTACGGTTA CCCAGCAGCC 
TCTTTTGCCG CTCCGTGGAG TGGGACGGGG GGCCCAGCGC AGCCGCCGCT CGGCCAGTTC 
CAGCCCGTAG GCGCCGCGTC TTTGCAAAGC TTCAACATCA GCAATTTGCA AAAATCGATC 
AGCAACCCTC CAGGCTCCAA CCTGCGGACC ACTTAG