LOC105217974 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105217974
Entrez Gene ID	105217974
Full Name	superkiller viralicidic activity 2-like 2 (S. cerevisiae)
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011193266.1	XP_011191568.1	superkiller viralicidic activity 2-like 2
XM_011193266.2	XP_011191568.1	exosome RNA helicase MTR4

Pathways from BioSystems
Gene Ontology

The following LOC105217974 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105217974 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa205055	XM_011193266.2 Latest version!	Zeugodacus cucurbitae exosome RNA helicase MTR4 (LOC105217974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBa205055	XM_011193266.1	Bactrocera cucurbitae superkiller viralicidic activity 2-like 2 (LOC105217974), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105217974 ( XM_011193266.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191568.1 Bactrocera cucurbitae superkiller viralicidic activity 2-like 2 (LOC105217974), mRNA.

Price & Availability↑

CloneID	OBa205055
Clone ID Related Accession (Same CDS sequence)	XM_011193266.1 , XM_011193266.2
Accession Version	XM_011193266.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	superkiller viralicidic activity 2-like 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAG TTGACGATTT ATTTGATTGT TTTAATGAAG ATAACGACGA AAGCGTTTCC 
GCCAATCCAG TTGTAGAGTT AAAAGACATT AAAACAGGAG ATTCTTTAGA GGAGAGGAAA 
GCTGAAAGCG GAGAAAAGCG TGGATGTGAG GCTATTAAAG CAGACGCAGA AAATGATGAG 
GCTCCGCAAA AACGTATAAA AGAGACGGAA TCCATATTGG ACGATATTTG TGTGGACGCC 
TTACGTTCCC GAATATCAGT GCACGTTATT GAGAGTCCAG AGTCGTGTAC ACATGAAGTA 
GCCGTATATC CGGGTCAAGC ATATATTCCT CTACAAGCCC AAAATGGACC ACCAGCAAAA 
GAATATCCTT TTGTATTAGA TCCTTTTCAA AAAGAAGCAA TTCTTTGCAT AGATAATCAA 
CAAAGTGTAT TAGTGTCTGC GCACACATCT GCTGGCAAAA CTGTAGTGGC CGAATATGCT 
ATTGCTAAAT CTCTGGGTTG TAAACAGCGT GTAATTTATA CCACACCTAT TAAAGCGCTT 
TCTAACCAAA AATATCGAGA GTTCAATGAA GAATTTAAAG ATGTTGGTTT GGTAACTGGT 
GATGTGACGA TAAATCCATC AGCTTCTTGC CTTATTATGA CAACGGAGAT TTTGCGGAAT 
ATGTTATATA GAGGAAGTGA GATTATGCGT GAAGTAGGTT GGGTCGTATT CGATGAAATT 
CATTACATGC GTGACAAAGA AAGAGGTGTA GTGTGGGAGG AAACCTTAAT TTTATTGCCC 
GACAATGTAC GCTACGTATT TCTTTCTGCT ACTATACCAA ATGCTCGCCA ATTCGCCGAA 
TGGGTCTGTC ATCTTCACAA GCAACCATGC CATGTAGTTT ACACAGATTA TCGGCCGACG 
CCGCTGCAAC ATTACATATT TCCAGCTGGT GGCGACGGTA TACATCTCAT AGTTGACGAA 
AAAGGCCAGT TTAAGGAAGA TAATTTTACA ACAGCCATGG CGGTTTTGCA AAATGCAGGT 
GATGCAGCTA AAGGCGATCA AAGAGGAAGA AAAGGTGGTA TTCGTGGTGT TGACAGTGGG 
CAGTCGAATA TTTTTAAAAT AGTCAAAATG ATAATGGAAC GGAATTTCGC CCCTGTAATT 
ATCTTTTCAT TTAGTAAAAA GGATTGTGAG GTATATGCTA TGCAAATGGC AAAGCTGGAT 
TTTAATACTG CCGAAGAGAA AAAACTGGTA GATGAAGTTT TCAATAATGC GATGGATGTA 
CTCTCTGAGG AAGATCGTCA ATTGCCGCAA GTTGAAAATG TTTTGCCTTT ACTAAAGCGT 
GGCATTGGTA TACATCATGG CGGTCTCTTG CCGATCCTAA AAGAAATTAT TGAGATTTTG 
TTTGGCGAAG GCTTGCTTAA AGCACTTTTT GCTACGGAAA CTTTTGCCAT GGGTTTGAAC 
ATGCCTGCGC GCACAGTACT TTTTACAGCA CCACGAAAAT TTGATGGTAA AAATTTCAGG 
TGGATTTCCT CTGGCGAATA CATCCAAATG GCTGGACGTG CTGGTCGACG TGGTCTCGAT 
GATAAAGGTA TCGTTATATT AATGATTGAC GAGAAAGTGT CACCATCCAT CGGTCGCGGA 
ATTGTACAAG GCAAAGCAGA TCCCATAAAC TCTGCATTTC ACTTAACTTA CAACATGGTG 
CTGAATTTGC TGCGTGTCGA AGAAATCAAT CCCGAATATA TGTTGGAACG TTCATTTTTT 
CAGTTCCAAA ACCAGTCGTC AATACCTGAA CTTTACAAAC AAGTTCAAGA CAAACAAAAT 
GAGTTGGCAA AAATTAAAAT CAGCGAAGAA CACAGCGTTG CATCCTACCA CCATATACGC 
GAACAACTCG ATTCTTATGG CAAACAGTTT AAGCGTTGGA TTACAAAACC CGAATATCTG 
GTGCCTTTCT TACAACCAGG TCGTCTGATA AAAATTCAAA ATGAAAAGGA CGAATTCGAT 
TGGGGTATTG TCGTGAACTT TAAAAAACAA ACAAATAATG CTAAAAATCC TCTGAAAGCA 
GAAACGGTCG TGGTAGTCGA TGTTTTATTG CATGTTACTG AGGATTCGGC AAAGGCCGAA 
GAGCCAAAAC CATGCAAGTC CGGCCAAAAG GGCAATATGG AAGTTGTTCC TGTTCTACAT 
ACACTTATAT CGCAGATTTC GTCTCTTCGC GTTTATTATC CCAATGACTT GCGTCCGGCG 
GATAACCGAC GAAGTGTGCT GAAAACGATC GCTGAAGTGA AGAAGCGTTT CCCAAAAGGC 
CCGCCACTGC TCAATCCCAT TACTGAAATG AACATAAAAA GTGATGAATT CAAACAGGTT 
GTGGACTCCA TAGATAAGTT CGAAGAGCGT TTGTTCGCAC ATCCTCTACA TAATTCGACT 
GAATTGGACA AGATTTACGA ACGTTATACA CAGAAATTGA AGGTGCAGGA AGAACTGAAG 
GCGGTTAAAA CAAAATTGAA GGAAGCACGT AGTTTGCTAC AGATGGACGA GCTGAAGCAT 
CGGAAACGTG TACTACGCCG TATGGAGTAC TGCACAGCTG CTGATGTAAT AGAGTTTAAA 
GGACGTGTTG CATGCGAATT AAGCTCAGCA GATGAACTTC TTTTAACTGA AATGATATTC 
AACGGGATAT TTAATGAAAT TACCACAGCA CAGTCAGTTG CATTGCTGTC TTGTTTCGTC 
TGCGATGAAA AGTCTTCAGA AACTCCCAAG GCAACCGAAG AACTTTCGGG TCCACTGCGT 
TCAATGCAAG ACTTGGCGCG TCGCATTGCA AAAATCTCCA CCGAATGCAA ATTAAATATG 
GACGAGGAAT CGTACGTGGA AAAATTCAAA CCATTTCTTA TGGACGTAGT TTTAGCGTGG 
TGTAAAGGTG CTTCATTTTT GAGCGTCTGC AGAATGACAG ACATTTTTGA AGGCTCCATA 
ATTCGATGTA TGCGCCGTCT GGAAGAATTA TTGCGGCAAT TGTGCCAAGC AGCGAAAACA 
ATCGGTAACA CAGACTTGGA AAACAAATTC TCTGAAGGTG TGCGCCTGCT CAAAAGGGAT 
ATTGTATTCG CCGCTTCATT ATATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191568.1
CDS	80..3169
Translation	MAEVDDLFDCFNEDNDESVSANPVVELKDIKTGDSLEERKAESGEKRGCEAIKADAENDEAPQKRIKETESILDDICVDALRSRISVHVIESPESCTHEVAVYPGQAYIPLQAQNGPPAKEYPFVLDPFQKEAILCIDNQQSVLVSAHTSAGKTVVAEYAIAKSLGCKQRVIYTTPIKALSNQKYREFNEEFKDVGLVTGDVTINPSASCLIMTTEILRNMLYRGSEIMREVGWVVFDEIHYMRDKERGVVWEETLILLPDNVRYVFLSATIPNARQFAEWVCHLHKQPCHVVYTDYRPTPLQHYIFPAGGDGIHLIVDEKGQFKEDNFTTAMAVLQNAGDAAKGDQRGRKGGIRGVDSGQSNIFKIVKMIMERNFAPVIIFSFSKKDCEVYAMQMAKLDFNTAEEKKLVDEVFNNAMDVLSEEDRQLPQVENVLPLLKRGIGIHHGGLLPILKEIIEILFGEGLLKALFATETFAMGLNMPARTVLFTAPRKFDGKNFRWISSGEYIQMAGRAGRRGLDDKGIVILMIDEKVSPSIGRGIVQGKADPINSAFHLTYNMVLNLLRVEEINPEYMLERSFFQFQNQSSIPELYKQVQDKQNELAKIKISEEHSVASYHHIREQLDSYGKQFKRWITKPEYLVPFLQPGRLIKIQNEKDEFDWGIVVNFKKQTNNAKNPLKAETVVVVDVLLHVTEDSAKAEEPKPCKSGQKGNMEVVPVLHTLISQISSLRVYYPNDLRPADNRRSVLKTIAEVKKRFPKGPPLLNPITEMNIKSDEFKQVVDSIDKFEERLFAHPLHNSTELDKIYERYTQKLKVQEELKAVKTKLKEARSLLQMDELKHRKRVLRRMEYCTAADVIEFKGRVACELSSADELLLTEMIFNGIFNEITTAQSVALLSCFVCDEKSSETPKATEELSGPLRSMQDLARRIAKISTECKLNMDEESYVEKFKPFLMDVVLAWCKGASFLSVCRMTDIFEGSIIRCMRRLEELLRQLCQAAKTIGNTDLENKFSEGVRLLKRDIVFAASLYL

Target ORF information:

RefSeq Version	XM_011193266.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae superkiller viralicidic activity 2-like 2 (LOC105217974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193266.1

ORF Insert Sequence:

ATGGCTGAAG TTGACGATTT ATTTGATTGT TTTAATGAAG ATAACGACGA AAGCGTTTCC 
GCCAATCCAG TTGTAGAGTT AAAAGACATT AAAACAGGAG ATTCTTTAGA GGAGAGGAAA 
GCTGAAAGCG GAGAAAAGCG TGGATGTGAG GCTATTAAAG CAGACGCAGA AAATGATGAG 
GCTCCGCAAA AACGTATAAA AGAGACGGAA TCCATATTGG ACGATATTTG TGTGGACGCC 
TTACGTTCCC GAATATCAGT GCACGTTATT GAGAGTCCAG AGTCGTGTAC ACATGAAGTA 
GCCGTATATC CGGGTCAAGC ATATATTCCT CTACAAGCCC AAAATGGACC ACCAGCAAAA 
GAATATCCTT TTGTATTAGA TCCTTTTCAA AAAGAAGCAA TTCTTTGCAT AGATAATCAA 
CAAAGTGTAT TAGTGTCTGC GCACACATCT GCTGGCAAAA CTGTAGTGGC CGAATATGCT 
ATTGCTAAAT CTCTGGGTTG TAAACAGCGT GTAATTTATA CCACACCTAT TAAAGCGCTT 
TCTAACCAAA AATATCGAGA GTTCAATGAA GAATTTAAAG ATGTTGGTTT GGTAACTGGT 
GATGTGACGA TAAATCCATC AGCTTCTTGC CTTATTATGA CAACGGAGAT TTTGCGGAAT 
ATGTTATATA GAGGAAGTGA GATTATGCGT GAAGTAGGTT GGGTCGTATT CGATGAAATT 
CATTACATGC GTGACAAAGA AAGAGGTGTA GTGTGGGAGG AAACCTTAAT TTTATTGCCC 
GACAATGTAC GCTACGTATT TCTTTCTGCT ACTATACCAA ATGCTCGCCA ATTCGCCGAA 
TGGGTCTGTC ATCTTCACAA GCAACCATGC CATGTAGTTT ACACAGATTA TCGGCCGACG 
CCGCTGCAAC ATTACATATT TCCAGCTGGT GGCGACGGTA TACATCTCAT AGTTGACGAA 
AAAGGCCAGT TTAAGGAAGA TAATTTTACA ACAGCCATGG CGGTTTTGCA AAATGCAGGT 
GATGCAGCTA AAGGCGATCA AAGAGGAAGA AAAGGTGGTA TTCGTGGTGT TGACAGTGGG 
CAGTCGAATA TTTTTAAAAT AGTCAAAATG ATAATGGAAC GGAATTTCGC CCCTGTAATT 
ATCTTTTCAT TTAGTAAAAA GGATTGTGAG GTATATGCTA TGCAAATGGC AAAGCTGGAT 
TTTAATACTG CCGAAGAGAA AAAACTGGTA GATGAAGTTT TCAATAATGC GATGGATGTA 
CTCTCTGAGG AAGATCGTCA ATTGCCGCAA GTTGAAAATG TTTTGCCTTT ACTAAAGCGT 
GGCATTGGTA TACATCATGG CGGTCTCTTG CCGATCCTAA AAGAAATTAT TGAGATTTTG 
TTTGGCGAAG GCTTGCTTAA AGCACTTTTT GCTACGGAAA CTTTTGCCAT GGGTTTGAAC 
ATGCCTGCGC GCACAGTACT TTTTACAGCA CCACGAAAAT TTGATGGTAA AAATTTCAGG 
TGGATTTCCT CTGGCGAATA CATCCAAATG GCTGGACGTG CTGGTCGACG TGGTCTCGAT 
GATAAAGGTA TCGTTATATT AATGATTGAC GAGAAAGTGT CACCATCCAT CGGTCGCGGA 
ATTGTACAAG GCAAAGCAGA TCCCATAAAC TCTGCATTTC ACTTAACTTA CAACATGGTG 
CTGAATTTGC TGCGTGTCGA AGAAATCAAT CCCGAATATA TGTTGGAACG TTCATTTTTT 
CAGTTCCAAA ACCAGTCGTC AATACCTGAA CTTTACAAAC AAGTTCAAGA CAAACAAAAT 
GAGTTGGCAA AAATTAAAAT CAGCGAAGAA CACAGCGTTG CATCCTACCA CCATATACGC 
GAACAACTCG ATTCTTATGG CAAACAGTTT AAGCGTTGGA TTACAAAACC CGAATATCTG 
GTGCCTTTCT TACAACCAGG TCGTCTGATA AAAATTCAAA ATGAAAAGGA CGAATTCGAT 
TGGGGTATTG TCGTGAACTT TAAAAAACAA ACAAATAATG CTAAAAATCC TCTGAAAGCA 
GAAACGGTCG TGGTAGTCGA TGTTTTATTG CATGTTACTG AGGATTCGGC AAAGGCCGAA 
GAGCCAAAAC CATGCAAGTC CGGCCAAAAG GGCAATATGG AAGTTGTTCC TGTTCTACAT 
ACACTTATAT CGCAGATTTC GTCTCTTCGC GTTTATTATC CCAATGACTT GCGTCCGGCG 
GATAACCGAC GAAGTGTGCT GAAAACGATC GCTGAAGTGA AGAAGCGTTT CCCAAAAGGC 
CCGCCACTGC TCAATCCCAT TACTGAAATG AACATAAAAA GTGATGAATT CAAACAGGTT 
GTGGACTCCA TAGATAAGTT CGAAGAGCGT TTGTTCGCAC ATCCTCTACA TAATTCGACT 
GAATTGGACA AGATTTACGA ACGTTATACA CAGAAATTGA AGGTGCAGGA AGAACTGAAG 
GCGGTTAAAA CAAAATTGAA GGAAGCACGT AGTTTGCTAC AGATGGACGA GCTGAAGCAT 
CGGAAACGTG TACTACGCCG TATGGAGTAC TGCACAGCTG CTGATGTAAT AGAGTTTAAA 
GGACGTGTTG CATGCGAATT AAGCTCAGCA GATGAACTTC TTTTAACTGA AATGATATTC 
AACGGGATAT TTAATGAAAT TACCACAGCA CAGTCAGTTG CATTGCTGTC TTGTTTCGTC 
TGCGATGAAA AGTCTTCAGA AACTCCCAAG GCAACCGAAG AACTTTCGGG TCCACTGCGT 
TCAATGCAAG ACTTGGCGCG TCGCATTGCA AAAATCTCCA CCGAATGCAA ATTAAATATG 
GACGAGGAAT CGTACGTGGA AAAATTCAAA CCATTTCTTA TGGACGTAGT TTTAGCGTGG 
TGTAAAGGTG CTTCATTTTT GAGCGTCTGC AGAATGACAG ACATTTTTGA AGGCTCCATA 
ATTCGATGTA TGCGCCGTCT GGAAGAATTA TTGCGGCAAT TGTGCCAAGC AGCGAAAACA 
ATCGGTAACA CAGACTTGGA AAACAAATTC TCTGAAGGTG TGCGCCTGCT CAAAAGGGAT 
ATTGTATTCG CCGCTTCATT ATATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217974 ( XM_011193266.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191568.1 Zeugodacus cucurbitae exosome RNA helicase MTR4 (LOC105217974), mRNA.

Price & Availability↑

CloneID	OBa205055
Clone ID Related Accession (Same CDS sequence)	XM_011193266.1 , XM_011193266.2
Accession Version	XM_011193266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	exosome RNA helicase MTR4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863835.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011193266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAG TTGACGATTT ATTTGATTGT TTTAATGAAG ATAACGACGA AAGCGTTTCC 
GCCAATCCAG TTGTAGAGTT AAAAGACATT AAAACAGGAG ATTCTTTAGA GGAGAGGAAA 
GCTGAAAGCG GAGAAAAGCG TGGATGTGAG GCTATTAAAG CAGACGCAGA AAATGATGAG 
GCTCCGCAAA AACGTATAAA AGAGACGGAA TCCATATTGG ACGATATTTG TGTGGACGCC 
TTACGTTCCC GAATATCAGT GCACGTTATT GAGAGTCCAG AGTCGTGTAC ACATGAAGTA 
GCCGTATATC CGGGTCAAGC ATATATTCCT CTACAAGCCC AAAATGGACC ACCAGCAAAA 
GAATATCCTT TTGTATTAGA TCCTTTTCAA AAAGAAGCAA TTCTTTGCAT AGATAATCAA 
CAAAGTGTAT TAGTGTCTGC GCACACATCT GCTGGCAAAA CTGTAGTGGC CGAATATGCT 
ATTGCTAAAT CTCTGGGTTG TAAACAGCGT GTAATTTATA CCACACCTAT TAAAGCGCTT 
TCTAACCAAA AATATCGAGA GTTCAATGAA GAATTTAAAG ATGTTGGTTT GGTAACTGGT 
GATGTGACGA TAAATCCATC AGCTTCTTGC CTTATTATGA CAACGGAGAT TTTGCGGAAT 
ATGTTATATA GAGGAAGTGA GATTATGCGT GAAGTAGGTT GGGTCGTATT CGATGAAATT 
CATTACATGC GTGACAAAGA AAGAGGTGTA GTGTGGGAGG AAACCTTAAT TTTATTGCCC 
GACAATGTAC GCTACGTATT TCTTTCTGCT ACTATACCAA ATGCTCGCCA ATTCGCCGAA 
TGGGTCTGTC ATCTTCACAA GCAACCATGC CATGTAGTTT ACACAGATTA TCGGCCGACG 
CCGCTGCAAC ATTACATATT TCCAGCTGGT GGCGACGGTA TACATCTCAT AGTTGACGAA 
AAAGGCCAGT TTAAGGAAGA TAATTTTACA ACAGCCATGG CGGTTTTGCA AAATGCAGGT 
GATGCAGCTA AAGGCGATCA AAGAGGAAGA AAAGGTGGTA TTCGTGGTGT TGACAGTGGG 
CAGTCGAATA TTTTTAAAAT AGTCAAAATG ATAATGGAAC GGAATTTCGC CCCTGTAATT 
ATCTTTTCAT TTAGTAAAAA GGATTGTGAG GTATATGCTA TGCAAATGGC AAAGCTGGAT 
TTTAATACTG CCGAAGAGAA AAAACTGGTA GATGAAGTTT TCAATAATGC GATGGATGTA 
CTCTCTGAGG AAGATCGTCA ATTGCCGCAA GTTGAAAATG TTTTGCCTTT ACTAAAGCGT 
GGCATTGGTA TACATCATGG CGGTCTCTTG CCGATCCTAA AAGAAATTAT TGAGATTTTG 
TTTGGCGAAG GCTTGCTTAA AGCACTTTTT GCTACGGAAA CTTTTGCCAT GGGTTTGAAC 
ATGCCTGCGC GCACAGTACT TTTTACAGCA CCACGAAAAT TTGATGGTAA AAATTTCAGG 
TGGATTTCCT CTGGCGAATA CATCCAAATG GCTGGACGTG CTGGTCGACG TGGTCTCGAT 
GATAAAGGTA TCGTTATATT AATGATTGAC GAGAAAGTGT CACCATCCAT CGGTCGCGGA 
ATTGTACAAG GCAAAGCAGA TCCCATAAAC TCTGCATTTC ACTTAACTTA CAACATGGTG 
CTGAATTTGC TGCGTGTCGA AGAAATCAAT CCCGAATATA TGTTGGAACG TTCATTTTTT 
CAGTTCCAAA ACCAGTCGTC AATACCTGAA CTTTACAAAC AAGTTCAAGA CAAACAAAAT 
GAGTTGGCAA AAATTAAAAT CAGCGAAGAA CACAGCGTTG CATCCTACCA CCATATACGC 
GAACAACTCG ATTCTTATGG CAAACAGTTT AAGCGTTGGA TTACAAAACC CGAATATCTG 
GTGCCTTTCT TACAACCAGG TCGTCTGATA AAAATTCAAA ATGAAAAGGA CGAATTCGAT 
TGGGGTATTG TCGTGAACTT TAAAAAACAA ACAAATAATG CTAAAAATCC TCTGAAAGCA 
GAAACGGTCG TGGTAGTCGA TGTTTTATTG CATGTTACTG AGGATTCGGC AAAGGCCGAA 
GAGCCAAAAC CATGCAAGTC CGGCCAAAAG GGCAATATGG AAGTTGTTCC TGTTCTACAT 
ACACTTATAT CGCAGATTTC GTCTCTTCGC GTTTATTATC CCAATGACTT GCGTCCGGCG 
GATAACCGAC GAAGTGTGCT GAAAACGATC GCTGAAGTGA AGAAGCGTTT CCCAAAAGGC 
CCGCCACTGC TCAATCCCAT TACTGAAATG AACATAAAAA GTGATGAATT CAAACAGGTT 
GTGGACTCCA TAGATAAGTT CGAAGAGCGT TTGTTCGCAC ATCCTCTACA TAATTCGACT 
GAATTGGACA AGATTTACGA ACGTTATACA CAGAAATTGA AGGTGCAGGA AGAACTGAAG 
GCGGTTAAAA CAAAATTGAA GGAAGCACGT AGTTTGCTAC AGATGGACGA GCTGAAGCAT 
CGGAAACGTG TACTACGCCG TATGGAGTAC TGCACAGCTG CTGATGTAAT AGAGTTTAAA 
GGACGTGTTG CATGCGAATT AAGCTCAGCA GATGAACTTC TTTTAACTGA AATGATATTC 
AACGGGATAT TTAATGAAAT TACCACAGCA CAGTCAGTTG CATTGCTGTC TTGTTTCGTC 
TGCGATGAAA AGTCTTCAGA AACTCCCAAG GCAACCGAAG AACTTTCGGG TCCACTGCGT 
TCAATGCAAG ACTTGGCGCG TCGCATTGCA AAAATCTCCA CCGAATGCAA ATTAAATATG 
GACGAGGAAT CGTACGTGGA AAAATTCAAA CCATTTCTTA TGGACGTAGT TTTAGCGTGG 
TGTAAAGGTG CTTCATTTTT GAGCGTCTGC AGAATGACAG ACATTTTTGA AGGCTCCATA 
ATTCGATGTA TGCGCCGTCT GGAAGAATTA TTGCGGCAAT TGTGCCAAGC AGCGAAAACA 
ATCGGTAACA CAGACTTGGA AAACAAATTC TCTGAAGGTG TGCGCCTGCT CAAAAGGGAT 
ATTGTATTCG CCGCTTCATT ATATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191568.1
CDS	1..3090
Translation	MAEVDDLFDCFNEDNDESVSANPVVELKDIKTGDSLEERKAESGEKRGCEAIKADAENDEAPQKRIKETESILDDICVDALRSRISVHVIESPESCTHEVAVYPGQAYIPLQAQNGPPAKEYPFVLDPFQKEAILCIDNQQSVLVSAHTSAGKTVVAEYAIAKSLGCKQRVIYTTPIKALSNQKYREFNEEFKDVGLVTGDVTINPSASCLIMTTEILRNMLYRGSEIMREVGWVVFDEIHYMRDKERGVVWEETLILLPDNVRYVFLSATIPNARQFAEWVCHLHKQPCHVVYTDYRPTPLQHYIFPAGGDGIHLIVDEKGQFKEDNFTTAMAVLQNAGDAAKGDQRGRKGGIRGVDSGQSNIFKIVKMIMERNFAPVIIFSFSKKDCEVYAMQMAKLDFNTAEEKKLVDEVFNNAMDVLSEEDRQLPQVENVLPLLKRGIGIHHGGLLPILKEIIEILFGEGLLKALFATETFAMGLNMPARTVLFTAPRKFDGKNFRWISSGEYIQMAGRAGRRGLDDKGIVILMIDEKVSPSIGRGIVQGKADPINSAFHLTYNMVLNLLRVEEINPEYMLERSFFQFQNQSSIPELYKQVQDKQNELAKIKISEEHSVASYHHIREQLDSYGKQFKRWITKPEYLVPFLQPGRLIKIQNEKDEFDWGIVVNFKKQTNNAKNPLKAETVVVVDVLLHVTEDSAKAEEPKPCKSGQKGNMEVVPVLHTLISQISSLRVYYPNDLRPADNRRSVLKTIAEVKKRFPKGPPLLNPITEMNIKSDEFKQVVDSIDKFEERLFAHPLHNSTELDKIYERYTQKLKVQEELKAVKTKLKEARSLLQMDELKHRKRVLRRMEYCTAADVIEFKGRVACELSSADELLLTEMIFNGIFNEITTAQSVALLSCFVCDEKSSETPKATEELSGPLRSMQDLARRIAKISTECKLNMDEESYVEKFKPFLMDVVLAWCKGASFLSVCRMTDIFEGSIIRCMRRLEELLRQLCQAAKTIGNTDLENKFSEGVRLLKRDIVFAASLYL

Target ORF information:

RefSeq Version	XM_011193266.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae exosome RNA helicase MTR4 (LOC105217974), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193266.2

ORF Insert Sequence:

ATGGCTGAAG TTGACGATTT ATTTGATTGT TTTAATGAAG ATAACGACGA AAGCGTTTCC 
GCCAATCCAG TTGTAGAGTT AAAAGACATT AAAACAGGAG ATTCTTTAGA GGAGAGGAAA 
GCTGAAAGCG GAGAAAAGCG TGGATGTGAG GCTATTAAAG CAGACGCAGA AAATGATGAG 
GCTCCGCAAA AACGTATAAA AGAGACGGAA TCCATATTGG ACGATATTTG TGTGGACGCC 
TTACGTTCCC GAATATCAGT GCACGTTATT GAGAGTCCAG AGTCGTGTAC ACATGAAGTA 
GCCGTATATC CGGGTCAAGC ATATATTCCT CTACAAGCCC AAAATGGACC ACCAGCAAAA 
GAATATCCTT TTGTATTAGA TCCTTTTCAA AAAGAAGCAA TTCTTTGCAT AGATAATCAA 
CAAAGTGTAT TAGTGTCTGC GCACACATCT GCTGGCAAAA CTGTAGTGGC CGAATATGCT 
ATTGCTAAAT CTCTGGGTTG TAAACAGCGT GTAATTTATA CCACACCTAT TAAAGCGCTT 
TCTAACCAAA AATATCGAGA GTTCAATGAA GAATTTAAAG ATGTTGGTTT GGTAACTGGT 
GATGTGACGA TAAATCCATC AGCTTCTTGC CTTATTATGA CAACGGAGAT TTTGCGGAAT 
ATGTTATATA GAGGAAGTGA GATTATGCGT GAAGTAGGTT GGGTCGTATT CGATGAAATT 
CATTACATGC GTGACAAAGA AAGAGGTGTA GTGTGGGAGG AAACCTTAAT TTTATTGCCC 
GACAATGTAC GCTACGTATT TCTTTCTGCT ACTATACCAA ATGCTCGCCA ATTCGCCGAA 
TGGGTCTGTC ATCTTCACAA GCAACCATGC CATGTAGTTT ACACAGATTA TCGGCCGACG 
CCGCTGCAAC ATTACATATT TCCAGCTGGT GGCGACGGTA TACATCTCAT AGTTGACGAA 
AAAGGCCAGT TTAAGGAAGA TAATTTTACA ACAGCCATGG CGGTTTTGCA AAATGCAGGT 
GATGCAGCTA AAGGCGATCA AAGAGGAAGA AAAGGTGGTA TTCGTGGTGT TGACAGTGGG 
CAGTCGAATA TTTTTAAAAT AGTCAAAATG ATAATGGAAC GGAATTTCGC CCCTGTAATT 
ATCTTTTCAT TTAGTAAAAA GGATTGTGAG GTATATGCTA TGCAAATGGC AAAGCTGGAT 
TTTAATACTG CCGAAGAGAA AAAACTGGTA GATGAAGTTT TCAATAATGC GATGGATGTA 
CTCTCTGAGG AAGATCGTCA ATTGCCGCAA GTTGAAAATG TTTTGCCTTT ACTAAAGCGT 
GGCATTGGTA TACATCATGG CGGTCTCTTG CCGATCCTAA AAGAAATTAT TGAGATTTTG 
TTTGGCGAAG GCTTGCTTAA AGCACTTTTT GCTACGGAAA CTTTTGCCAT GGGTTTGAAC 
ATGCCTGCGC GCACAGTACT TTTTACAGCA CCACGAAAAT TTGATGGTAA AAATTTCAGG 
TGGATTTCCT CTGGCGAATA CATCCAAATG GCTGGACGTG CTGGTCGACG TGGTCTCGAT 
GATAAAGGTA TCGTTATATT AATGATTGAC GAGAAAGTGT CACCATCCAT CGGTCGCGGA 
ATTGTACAAG GCAAAGCAGA TCCCATAAAC TCTGCATTTC ACTTAACTTA CAACATGGTG 
CTGAATTTGC TGCGTGTCGA AGAAATCAAT CCCGAATATA TGTTGGAACG TTCATTTTTT 
CAGTTCCAAA ACCAGTCGTC AATACCTGAA CTTTACAAAC AAGTTCAAGA CAAACAAAAT 
GAGTTGGCAA AAATTAAAAT CAGCGAAGAA CACAGCGTTG CATCCTACCA CCATATACGC 
GAACAACTCG ATTCTTATGG CAAACAGTTT AAGCGTTGGA TTACAAAACC CGAATATCTG 
GTGCCTTTCT TACAACCAGG TCGTCTGATA AAAATTCAAA ATGAAAAGGA CGAATTCGAT 
TGGGGTATTG TCGTGAACTT TAAAAAACAA ACAAATAATG CTAAAAATCC TCTGAAAGCA 
GAAACGGTCG TGGTAGTCGA TGTTTTATTG CATGTTACTG AGGATTCGGC AAAGGCCGAA 
GAGCCAAAAC CATGCAAGTC CGGCCAAAAG GGCAATATGG AAGTTGTTCC TGTTCTACAT 
ACACTTATAT CGCAGATTTC GTCTCTTCGC GTTTATTATC CCAATGACTT GCGTCCGGCG 
GATAACCGAC GAAGTGTGCT GAAAACGATC GCTGAAGTGA AGAAGCGTTT CCCAAAAGGC 
CCGCCACTGC TCAATCCCAT TACTGAAATG AACATAAAAA GTGATGAATT CAAACAGGTT 
GTGGACTCCA TAGATAAGTT CGAAGAGCGT TTGTTCGCAC ATCCTCTACA TAATTCGACT 
GAATTGGACA AGATTTACGA ACGTTATACA CAGAAATTGA AGGTGCAGGA AGAACTGAAG 
GCGGTTAAAA CAAAATTGAA GGAAGCACGT AGTTTGCTAC AGATGGACGA GCTGAAGCAT 
CGGAAACGTG TACTACGCCG TATGGAGTAC TGCACAGCTG CTGATGTAAT AGAGTTTAAA 
GGACGTGTTG CATGCGAATT AAGCTCAGCA GATGAACTTC TTTTAACTGA AATGATATTC 
AACGGGATAT TTAATGAAAT TACCACAGCA CAGTCAGTTG CATTGCTGTC TTGTTTCGTC 
TGCGATGAAA AGTCTTCAGA AACTCCCAAG GCAACCGAAG AACTTTCGGG TCCACTGCGT 
TCAATGCAAG ACTTGGCGCG TCGCATTGCA AAAATCTCCA CCGAATGCAA ATTAAATATG 
GACGAGGAAT CGTACGTGGA AAAATTCAAA CCATTTCTTA TGGACGTAGT TTTAGCGTGG 
TGTAAAGGTG CTTCATTTTT GAGCGTCTGC AGAATGACAG ACATTTTTGA AGGCTCCATA 
ATTCGATGTA TGCGCCGTCT GGAAGAATTA TTGCGGCAAT TGTGCCAAGC AGCGAAAACA 
ATCGGTAACA CAGACTTGGA AAACAAATTC TCTGAAGGTG TGCGCCTGCT CAAAAGGGAT 
ATTGTATTCG CCGCTTCATT ATATTTATAA