LOC105217893 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105217893
Entrez Gene ID	105217893
Full Name	elongation factor 2
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011193133.2	XP_011191435.1	elongation factor 2
XM_011193133.1	XP_011191435.1	elongation factor 2

Pathways from BioSystems
Gene Ontology

The following LOC105217893 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105217893 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa204972	XM_011193133.2 Latest version!	Zeugodacus cucurbitae elongation factor 2 (Ef2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa204972	XM_011193133.1	Bactrocera cucurbitae elongation factor 2 (LOC105217893), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105217893 ( XM_011193133.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191435.1 Zeugodacus cucurbitae elongation factor 2 (Ef2b), mRNA.

Price & Availability↑

CloneID	OBa204972
Clone ID Related Accession (Same CDS sequence)	XM_011193133.2 , XM_011193133.1
Accession Version	XM_011193133.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	elongation factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863832.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011193133.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACT TTACTGTCGA CGAAATCCGT TCCTTGATGG ACAAGAAGCG GAACATCCGT 
AACATGTCCG TCATTGCTCA CGTCGATCAT GGCAAATCTA CTTTGACCGA CTCCTTGGTG 
TCGAAGGCTG GTATCATTGC TGGTGCCAAG GCTGGTGAAA CCCGTTTCAC TGACACCCGT 
AAGGATGAAC AGGAACGTTG CATTACCATC AAGTCAACTG CCATTTCTAT GTACTTCGAA 
GTTGAAGATA AGGATCTTGT CTTCATCACT CATGCCGATC AGCGTGAGAA GGACTGCAAG 
GGTTTCTTGA TCAATTTGAT CGATTCACCC GGTCACGTTG ATTTCTCATC TGAAGTAACA 
GCTGCTCTTC GCGTAACTGA CGGTGCCTTG GTCGTCGTCG ATTGCGTGTC TGGTGTATGT 
GTGCAAACTG AGACCGTGTT GCGTCAAGCC ATCGCTGAAC GTATTAAGCC AATTTTGTTC 
ATGAACAAGA TGGATCGCGC TTTGCTTGAA TTGCAATTGG GCGCTGAAGA ATTGTACCAG 
ACTTTCCAGC GTATCGTTGA AAACGTGAAC GTCATCATTG CTACTTATAA CGATGATGGT 
GGTCCAATGG GTGAAGTACG TGTCGACCCC TCAAAGGGTT CCGTCGGTTT CGGTTCCGGT 
CTGCACGGTT GGGCTTTCAC TCTTAAGCAG TTCTCCGAAA TGTACTCCGA GAAATTCAAG 
ATCGACGTTG TCAAGCTAAT GAACCGTCTG TGGGGTGAGA ACTTCTTCAA CGCCAAGACC 
AAGAAATGGC AAAAACAAAA GGAAGCCGAT AACAAGCGTT CCTTCTGCAT GTACATCTTG 
GATCCCATCT ACAAAGTGTT CGATGCTATC ATGAACTACA AGAAGGAAGA GATCGGTACC 
TTGTTGGAAA AGATCGGTGT TGCCATCAAG CACGAAGATA AGGACAAGGA TGGCAAAGCT 
TTGTTGAAGG TCGTTATGCG CACATGGTTG CCAGCTGGTG AAGCTCTGCT TCAGATGATT 
GCCATTCACT TGCCTTCACC TGTGGTTGCT CAGAAATACC GTATGGAGAT GTTGTACGAG 
GGTCCACATG ACGATGAGGC TGCCGTCGCC GTTAAGAACT GTGATCCCGA TGGTCCATTG 
ATGATGTACA TTTCCAAAAT GGTACCGACA TCCGATAAGG GTCGTTTCTA TGCCTTCGGT 
CGTGTCTTCT CCGGTAAGGT CGCTACTGGC CAGAAGTGCC GTATCATGGG CCCCAACTAT 
GTTCCCGGCA AGAAGGAAGA TTTGTACGAA AAGTCCATCC AACGTACTAT TTTGATGATG 
GGTCGTTATG TTGAAGCCAT CGAAGATGTG CCTTCCGGTA ACATTTGCGG TCTCGTCGGT 
GTCGATCAGT TCTTGGTGAA GACCGGTACC ATCACCACAT TCAAGGATGC CCACAACATG 
AAGGTGATGA AATTCTCCGT GTCACCTGTC GTGCGTGTCG CTGTCGAGCC CAAGAACCCT 
GCTGATTTGC CAAAATTGGT GGAAGGTCTT AAGCGTTTGG CCAAATCCGA TCCTATGGTG 
CAATGTATCA TTGAAGAGTC TGGCGAGCAT ATCATTGCTG GTGCCGGTGA ATTGCATTTG 
GAAATTTGCT TGAAGGATTT GGAAGAAGAT CACGCTTGCA TTCCATTGAA GAAATCCGAT 
CCCGTTGTAT CATACCGTGA AACCGTATTC GAGGAATCCA ACCAAATGTG CTTGTCCAAA 
TCGCCCAACA AGCATAACCG TTTGATGATG AAGGCTTTGC CCATGCCCGA CGGTTTGCCC 
GAGGACATTG ATAACGGTGA TGTAAGCTCC AAGGACGATT TCAAATTGCG TGCCCGTTAC 
TTGGCTGAGA AATACGATTA TGATGTGACA GAAGCCCGTA AGATCTGGTG TTTCGGTCCT 
GACGGTACTG GCCCTAACTT CGTATTGGAT TGTACCAAAT CCGTACAATA CTTGAATGAA 
ATCAAGGATT CCGTTGTTGC TGGTTTCCAA TGGGCATCCA AGGAAGGTAT CATGGCTGAA 
GAGAACTTGC GTGGTGTACG TTTCAACATT TATGATGTGA CGTTGCACGC TGATGCTATC 
CATCGTGGTG GTGGTCAAAT CATTCCAACA ACTCGTCGTT GCTTGTACGC TTCAACAATT 
ACAGCTGGAC CACGTTTGAT GGAACCCGTT TACTTGTGCG AAATCCAATG TCCCGAAGTT 
GCTGTCGGTG GTATCTACGG TGTGTTGAAT AGACGTCGTG GTCATGTTTT CGAAGAAGCT 
CAAGTTGTTG GTACACCTAT GTTCGTTGTC AAGGCTTACT TGCCCGTAAA CGAGTCGTTC 
GGTTTCACCG CCGATTTGCG TTCCAACACC GGTGGACAAG CTTTCCCACA ATGCGTGTTC 
GACCATTGGC AAGTGTTGCC CGGTGATCCG TGCGAACCCA GCAGCAAACC ATACCAGATT 
GTACAGGATA CACGTAAGCG TAAGGGTTTG AAGGAAGGTC TACCCGACTT GTCCCAATAC 
CTCGATAAAT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191435.1
CDS	149..2683
Translation	MVNFTVDEIRSLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQERCITIKSTAISMYFEVEDKDLVFITHADQREKDCKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLGAEELYQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKTKKWQKQKEADNKRSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVAIKHEDKDKDGKALLKVVMRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAVAVKNCDPDGPLMMYISKMVPTSDKGRFYAFGRVFSGKVATGQKCRIMGPNYVPGKKEDLYEKSIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVFEESNQMCLSKSPNKHNRLMMKALPMPDGLPEDIDNGDVSSKDDFKLRARYLAEKYDYDVTEARKIWCFGPDGTGPNFVLDCTKSVQYLNEIKDSVVAGFQWASKEGIMAEENLRGVRFNIYDVTLHADAIHRGGGQIIPTTRRCLYASTITAGPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEEAQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPSSKPYQIVQDTRKRKGLKEGLPDLSQYLDKL

Target ORF information:

RefSeq Version	XM_011193133.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae elongation factor 2 (Ef2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193133.2

ORF Insert Sequence:

ATGGTCAACT TTACTGTCGA CGAAATCCGT TCCTTGATGG ACAAGAAGCG GAACATCCGT 
AACATGTCCG TCATTGCTCA CGTCGATCAT GGCAAATCTA CTTTGACCGA CTCCTTGGTG 
TCGAAGGCTG GTATCATTGC TGGTGCCAAG GCTGGTGAAA CCCGTTTCAC TGACACCCGT 
AAGGATGAAC AGGAACGTTG CATTACCATC AAGTCAACTG CCATTTCTAT GTACTTCGAA 
GTTGAAGATA AGGATCTTGT CTTCATCACT CATGCCGATC AGCGTGAGAA GGACTGCAAG 
GGTTTCTTGA TCAATTTGAT CGATTCACCC GGTCACGTTG ATTTCTCATC TGAAGTAACA 
GCTGCTCTTC GCGTAACTGA CGGTGCCTTG GTCGTCGTCG ATTGCGTGTC TGGTGTATGT 
GTGCAAACTG AGACCGTGTT GCGTCAAGCC ATCGCTGAAC GTATTAAGCC AATTTTGTTC 
ATGAACAAGA TGGATCGCGC TTTGCTTGAA TTGCAATTGG GCGCTGAAGA ATTGTACCAG 
ACTTTCCAGC GTATCGTTGA AAACGTGAAC GTCATCATTG CTACTTATAA CGATGATGGT 
GGTCCAATGG GTGAAGTACG TGTCGACCCC TCAAAGGGTT CCGTCGGTTT CGGTTCCGGT 
CTGCACGGTT GGGCTTTCAC TCTTAAGCAG TTCTCCGAAA TGTACTCCGA GAAATTCAAG 
ATCGACGTTG TCAAGCTAAT GAACCGTCTG TGGGGTGAGA ACTTCTTCAA CGCCAAGACC 
AAGAAATGGC AAAAACAAAA GGAAGCCGAT AACAAGCGTT CCTTCTGCAT GTACATCTTG 
GATCCCATCT ACAAAGTGTT CGATGCTATC ATGAACTACA AGAAGGAAGA GATCGGTACC 
TTGTTGGAAA AGATCGGTGT TGCCATCAAG CACGAAGATA AGGACAAGGA TGGCAAAGCT 
TTGTTGAAGG TCGTTATGCG CACATGGTTG CCAGCTGGTG AAGCTCTGCT TCAGATGATT 
GCCATTCACT TGCCTTCACC TGTGGTTGCT CAGAAATACC GTATGGAGAT GTTGTACGAG 
GGTCCACATG ACGATGAGGC TGCCGTCGCC GTTAAGAACT GTGATCCCGA TGGTCCATTG 
ATGATGTACA TTTCCAAAAT GGTACCGACA TCCGATAAGG GTCGTTTCTA TGCCTTCGGT 
CGTGTCTTCT CCGGTAAGGT CGCTACTGGC CAGAAGTGCC GTATCATGGG CCCCAACTAT 
GTTCCCGGCA AGAAGGAAGA TTTGTACGAA AAGTCCATCC AACGTACTAT TTTGATGATG 
GGTCGTTATG TTGAAGCCAT CGAAGATGTG CCTTCCGGTA ACATTTGCGG TCTCGTCGGT 
GTCGATCAGT TCTTGGTGAA GACCGGTACC ATCACCACAT TCAAGGATGC CCACAACATG 
AAGGTGATGA AATTCTCCGT GTCACCTGTC GTGCGTGTCG CTGTCGAGCC CAAGAACCCT 
GCTGATTTGC CAAAATTGGT GGAAGGTCTT AAGCGTTTGG CCAAATCCGA TCCTATGGTG 
CAATGTATCA TTGAAGAGTC TGGCGAGCAT ATCATTGCTG GTGCCGGTGA ATTGCATTTG 
GAAATTTGCT TGAAGGATTT GGAAGAAGAT CACGCTTGCA TTCCATTGAA GAAATCCGAT 
CCCGTTGTAT CATACCGTGA AACCGTATTC GAGGAATCCA ACCAAATGTG CTTGTCCAAA 
TCGCCCAACA AGCATAACCG TTTGATGATG AAGGCTTTGC CCATGCCCGA CGGTTTGCCC 
GAGGACATTG ATAACGGTGA TGTAAGCTCC AAGGACGATT TCAAATTGCG TGCCCGTTAC 
TTGGCTGAGA AATACGATTA TGATGTGACA GAAGCCCGTA AGATCTGGTG TTTCGGTCCT 
GACGGTACTG GCCCTAACTT CGTATTGGAT TGTACCAAAT CCGTACAATA CTTGAATGAA 
ATCAAGGATT CCGTTGTTGC TGGTTTCCAA TGGGCATCCA AGGAAGGTAT CATGGCTGAA 
GAGAACTTGC GTGGTGTACG TTTCAACATT TATGATGTGA CGTTGCACGC TGATGCTATC 
CATCGTGGTG GTGGTCAAAT CATTCCAACA ACTCGTCGTT GCTTGTACGC TTCAACAATT 
ACAGCTGGAC CACGTTTGAT GGAACCCGTT TACTTGTGCG AAATCCAATG TCCCGAAGTT 
GCTGTCGGTG GTATCTACGG TGTGTTGAAT AGACGTCGTG GTCATGTTTT CGAAGAAGCT 
CAAGTTGTTG GTACACCTAT GTTCGTTGTC AAGGCTTACT TGCCCGTAAA CGAGTCGTTC 
GGTTTCACCG CCGATTTGCG TTCCAACACC GGTGGACAAG CTTTCCCACA ATGCGTGTTC 
GACCATTGGC AAGTGTTGCC CGGTGATCCG TGCGAACCCA GCAGCAAACC ATACCAGATT 
GTACAGGATA CACGTAAGCG TAAGGGTTTG AAGGAAGGTC TACCCGACTT GTCCCAATAC 
CTCGATAAAT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105217893 ( XM_011193133.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011191435.1 Bactrocera cucurbitae elongation factor 2 (LOC105217893), mRNA.

Price & Availability↑

CloneID	OBa204972
Clone ID Related Accession (Same CDS sequence)	XM_011193133.2 , XM_011193133.1
Accession Version	XM_011193133.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	elongation factor 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863832.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACT TTACTGTCGA CGAAATCCGT TCCTTGATGG ACAAGAAGCG GAACATCCGT 
AACATGTCCG TCATTGCTCA CGTCGATCAT GGCAAATCTA CTTTGACCGA CTCCTTGGTG 
TCGAAGGCTG GTATCATTGC TGGTGCCAAG GCTGGTGAAA CCCGTTTCAC TGACACCCGT 
AAGGATGAAC AGGAACGTTG CATTACCATC AAGTCAACTG CCATTTCTAT GTACTTCGAA 
GTTGAAGATA AGGATCTTGT CTTCATCACT CATGCCGATC AGCGTGAGAA GGACTGCAAG 
GGTTTCTTGA TCAATTTGAT CGATTCACCC GGTCACGTTG ATTTCTCATC TGAAGTAACA 
GCTGCTCTTC GCGTAACTGA CGGTGCCTTG GTCGTCGTCG ATTGCGTGTC TGGTGTATGT 
GTGCAAACTG AGACCGTGTT GCGTCAAGCC ATCGCTGAAC GTATTAAGCC AATTTTGTTC 
ATGAACAAGA TGGATCGCGC TTTGCTTGAA TTGCAATTGG GCGCTGAAGA ATTGTACCAG 
ACTTTCCAGC GTATCGTTGA AAACGTGAAC GTCATCATTG CTACTTATAA CGATGATGGT 
GGTCCAATGG GTGAAGTACG TGTCGACCCC TCAAAGGGTT CCGTCGGTTT CGGTTCCGGT 
CTGCACGGTT GGGCTTTCAC TCTTAAGCAG TTCTCCGAAA TGTACTCCGA GAAATTCAAG 
ATCGACGTTG TCAAGCTAAT GAACCGTCTG TGGGGTGAGA ACTTCTTCAA CGCCAAGACC 
AAGAAATGGC AAAAACAAAA GGAAGCCGAT AACAAGCGTT CCTTCTGCAT GTACATCTTG 
GATCCCATCT ACAAAGTGTT CGATGCTATC ATGAACTACA AGAAGGAAGA GATCGGTACC 
TTGTTGGAAA AGATCGGTGT TGCCATCAAG CACGAAGATA AGGACAAGGA TGGCAAAGCT 
TTGTTGAAGG TCGTTATGCG CACATGGTTG CCAGCTGGTG AAGCTCTGCT TCAGATGATT 
GCCATTCACT TGCCTTCACC TGTGGTTGCT CAGAAATACC GTATGGAGAT GTTGTACGAG 
GGTCCACATG ACGATGAGGC TGCCGTCGCC GTTAAGAACT GTGATCCCGA TGGTCCATTG 
ATGATGTACA TTTCCAAAAT GGTACCGACA TCCGATAAGG GTCGTTTCTA TGCCTTCGGT 
CGTGTCTTCT CCGGTAAGGT CGCTACTGGC CAGAAGTGCC GTATCATGGG CCCCAACTAT 
GTTCCCGGCA AGAAGGAAGA TTTGTACGAA AAGTCCATCC AACGTACTAT TTTGATGATG 
GGTCGTTATG TTGAAGCCAT CGAAGATGTG CCTTCCGGTA ACATTTGCGG TCTCGTCGGT 
GTCGATCAGT TCTTGGTGAA GACCGGTACC ATCACCACAT TCAAGGATGC CCACAACATG 
AAGGTGATGA AATTCTCCGT GTCACCTGTC GTGCGTGTCG CTGTCGAGCC CAAGAACCCT 
GCTGATTTGC CAAAATTGGT GGAAGGTCTT AAGCGTTTGG CCAAATCCGA TCCTATGGTG 
CAATGTATCA TTGAAGAGTC TGGCGAGCAT ATCATTGCTG GTGCCGGTGA ATTGCATTTG 
GAAATTTGCT TGAAGGATTT GGAAGAAGAT CACGCTTGCA TTCCATTGAA GAAATCCGAT 
CCCGTTGTAT CATACCGTGA AACCGTATTC GAGGAATCCA ACCAAATGTG CTTGTCCAAA 
TCGCCCAACA AGCATAACCG TTTGATGATG AAGGCTTTGC CCATGCCCGA CGGTTTGCCC 
GAGGACATTG ATAACGGTGA TGTAAGCTCC AAGGACGATT TCAAATTGCG TGCCCGTTAC 
TTGGCTGAGA AATACGATTA TGATGTGACA GAAGCCCGTA AGATCTGGTG TTTCGGTCCT 
GACGGTACTG GCCCTAACTT CGTATTGGAT TGTACCAAAT CCGTACAATA CTTGAATGAA 
ATCAAGGATT CCGTTGTTGC TGGTTTCCAA TGGGCATCCA AGGAAGGTAT CATGGCTGAA 
GAGAACTTGC GTGGTGTACG TTTCAACATT TATGATGTGA CGTTGCACGC TGATGCTATC 
CATCGTGGTG GTGGTCAAAT CATTCCAACA ACTCGTCGTT GCTTGTACGC TTCAACAATT 
ACAGCTGGAC CACGTTTGAT GGAACCCGTT TACTTGTGCG AAATCCAATG TCCCGAAGTT 
GCTGTCGGTG GTATCTACGG TGTGTTGAAT AGACGTCGTG GTCATGTTTT CGAAGAAGCT 
CAAGTTGTTG GTACACCTAT GTTCGTTGTC AAGGCTTACT TGCCCGTAAA CGAGTCGTTC 
GGTTTCACCG CCGATTTGCG TTCCAACACC GGTGGACAAG CTTTCCCACA ATGCGTGTTC 
GACCATTGGC AAGTGTTGCC CGGTGATCCG TGCGAACCCA GCAGCAAACC ATACCAGATT 
GTACAGGATA CACGTAAGCG TAAGGGTTTG AAGGAAGGTC TACCCGACTT GTCCCAATAC 
CTCGATAAAT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011191435.1
CDS	102..2636
Translation	MVNFTVDEIRSLMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGAKAGETRFTDTRKDEQERCITIKSTAISMYFEVEDKDLVFITHADQREKDCKGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPILFMNKMDRALLELQLGAEELYQTFQRIVENVNVIIATYNDDGGPMGEVRVDPSKGSVGFGSGLHGWAFTLKQFSEMYSEKFKIDVVKLMNRLWGENFFNAKTKKWQKQKEADNKRSFCMYILDPIYKVFDAIMNYKKEEIGTLLEKIGVAIKHEDKDKDGKALLKVVMRTWLPAGEALLQMIAIHLPSPVVAQKYRMEMLYEGPHDDEAAVAVKNCDPDGPLMMYISKMVPTSDKGRFYAFGRVFSGKVATGQKCRIMGPNYVPGKKEDLYEKSIQRTILMMGRYVEAIEDVPSGNICGLVGVDQFLVKTGTITTFKDAHNMKVMKFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVFEESNQMCLSKSPNKHNRLMMKALPMPDGLPEDIDNGDVSSKDDFKLRARYLAEKYDYDVTEARKIWCFGPDGTGPNFVLDCTKSVQYLNEIKDSVVAGFQWASKEGIMAEENLRGVRFNIYDVTLHADAIHRGGGQIIPTTRRCLYASTITAGPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEEAQVVGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPSSKPYQIVQDTRKRKGLKEGLPDLSQYLDKL

Target ORF information:

RefSeq Version	XM_011193133.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae elongation factor 2 (LOC105217893), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193133.1

ORF Insert Sequence:

ATGGTCAACT TTACTGTCGA CGAAATCCGT TCCTTGATGG ACAAGAAGCG GAACATCCGT 
AACATGTCCG TCATTGCTCA CGTCGATCAT GGCAAATCTA CTTTGACCGA CTCCTTGGTG 
TCGAAGGCTG GTATCATTGC TGGTGCCAAG GCTGGTGAAA CCCGTTTCAC TGACACCCGT 
AAGGATGAAC AGGAACGTTG CATTACCATC AAGTCAACTG CCATTTCTAT GTACTTCGAA 
GTTGAAGATA AGGATCTTGT CTTCATCACT CATGCCGATC AGCGTGAGAA GGACTGCAAG 
GGTTTCTTGA TCAATTTGAT CGATTCACCC GGTCACGTTG ATTTCTCATC TGAAGTAACA 
GCTGCTCTTC GCGTAACTGA CGGTGCCTTG GTCGTCGTCG ATTGCGTGTC TGGTGTATGT 
GTGCAAACTG AGACCGTGTT GCGTCAAGCC ATCGCTGAAC GTATTAAGCC AATTTTGTTC 
ATGAACAAGA TGGATCGCGC TTTGCTTGAA TTGCAATTGG GCGCTGAAGA ATTGTACCAG 
ACTTTCCAGC GTATCGTTGA AAACGTGAAC GTCATCATTG CTACTTATAA CGATGATGGT 
GGTCCAATGG GTGAAGTACG TGTCGACCCC TCAAAGGGTT CCGTCGGTTT CGGTTCCGGT 
CTGCACGGTT GGGCTTTCAC TCTTAAGCAG TTCTCCGAAA TGTACTCCGA GAAATTCAAG 
ATCGACGTTG TCAAGCTAAT GAACCGTCTG TGGGGTGAGA ACTTCTTCAA CGCCAAGACC 
AAGAAATGGC AAAAACAAAA GGAAGCCGAT AACAAGCGTT CCTTCTGCAT GTACATCTTG 
GATCCCATCT ACAAAGTGTT CGATGCTATC ATGAACTACA AGAAGGAAGA GATCGGTACC 
TTGTTGGAAA AGATCGGTGT TGCCATCAAG CACGAAGATA AGGACAAGGA TGGCAAAGCT 
TTGTTGAAGG TCGTTATGCG CACATGGTTG CCAGCTGGTG AAGCTCTGCT TCAGATGATT 
GCCATTCACT TGCCTTCACC TGTGGTTGCT CAGAAATACC GTATGGAGAT GTTGTACGAG 
GGTCCACATG ACGATGAGGC TGCCGTCGCC GTTAAGAACT GTGATCCCGA TGGTCCATTG 
ATGATGTACA TTTCCAAAAT GGTACCGACA TCCGATAAGG GTCGTTTCTA TGCCTTCGGT 
CGTGTCTTCT CCGGTAAGGT CGCTACTGGC CAGAAGTGCC GTATCATGGG CCCCAACTAT 
GTTCCCGGCA AGAAGGAAGA TTTGTACGAA AAGTCCATCC AACGTACTAT TTTGATGATG 
GGTCGTTATG TTGAAGCCAT CGAAGATGTG CCTTCCGGTA ACATTTGCGG TCTCGTCGGT 
GTCGATCAGT TCTTGGTGAA GACCGGTACC ATCACCACAT TCAAGGATGC CCACAACATG 
AAGGTGATGA AATTCTCCGT GTCACCTGTC GTGCGTGTCG CTGTCGAGCC CAAGAACCCT 
GCTGATTTGC CAAAATTGGT GGAAGGTCTT AAGCGTTTGG CCAAATCCGA TCCTATGGTG 
CAATGTATCA TTGAAGAGTC TGGCGAGCAT ATCATTGCTG GTGCCGGTGA ATTGCATTTG 
GAAATTTGCT TGAAGGATTT GGAAGAAGAT CACGCTTGCA TTCCATTGAA GAAATCCGAT 
CCCGTTGTAT CATACCGTGA AACCGTATTC GAGGAATCCA ACCAAATGTG CTTGTCCAAA 
TCGCCCAACA AGCATAACCG TTTGATGATG AAGGCTTTGC CCATGCCCGA CGGTTTGCCC 
GAGGACATTG ATAACGGTGA TGTAAGCTCC AAGGACGATT TCAAATTGCG TGCCCGTTAC 
TTGGCTGAGA AATACGATTA TGATGTGACA GAAGCCCGTA AGATCTGGTG TTTCGGTCCT 
GACGGTACTG GCCCTAACTT CGTATTGGAT TGTACCAAAT CCGTACAATA CTTGAATGAA 
ATCAAGGATT CCGTTGTTGC TGGTTTCCAA TGGGCATCCA AGGAAGGTAT CATGGCTGAA 
GAGAACTTGC GTGGTGTACG TTTCAACATT TATGATGTGA CGTTGCACGC TGATGCTATC 
CATCGTGGTG GTGGTCAAAT CATTCCAACA ACTCGTCGTT GCTTGTACGC TTCAACAATT 
ACAGCTGGAC CACGTTTGAT GGAACCCGTT TACTTGTGCG AAATCCAATG TCCCGAAGTT 
GCTGTCGGTG GTATCTACGG TGTGTTGAAT AGACGTCGTG GTCATGTTTT CGAAGAAGCT 
CAAGTTGTTG GTACACCTAT GTTCGTTGTC AAGGCTTACT TGCCCGTAAA CGAGTCGTTC 
GGTTTCACCG CCGATTTGCG TTCCAACACC GGTGGACAAG CTTTCCCACA ATGCGTGTTC 
GACCATTGGC AAGTGTTGCC CGGTGATCCG TGCGAACCCA GCAGCAAACC ATACCAGATT 
GTACAGGATA CACGTAAGCG TAAGGGTTTG AAGGAAGGTC TACCCGACTT GTCCCAATAC 
CTCGATAAAT TGTAA