kif21b gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012970966.2	XP_012826420.1	kinesin-like protein KIF21B isoform X12
XM_004910720.4	XP_004910777.1	kinesin-like protein KIF21B isoform X1
XM_012970924.3	XP_012826378.1	kinesin-like protein KIF21B isoform X4
XM_012970944.3	XP_012826398.1	kinesin-like protein KIF21B isoform X8
XM_012970955.3	XP_012826409.1	kinesin-like protein KIF21B isoform X9
XM_012970924.2	XP_012826378.1	kinesin-like protein KIF21B isoform X4
XM_012970955.2	XP_012826409.1	kinesin-like protein KIF21B isoform X10
XM_004910720.3	XP_004910777.1	kinesin-like protein KIF21B isoform X1
XM_004910723.3	XP_004910780.1	kinesin-like protein KIF21B isoform X7
XM_004910721.4	XP_004910778.1	kinesin-like protein KIF21B isoform X2
XM_004910722.3	XP_004910779.1	kinesin-like protein KIF21B isoform X6
XM_012970944.2	XP_012826398.1	kinesin-like protein KIF21B isoform X8
XM_012970952.2	XP_012826406.1	kinesin-like protein KIF21B isoform X9
XM_012970961.2	XP_012826415.1	kinesin-like protein KIF21B isoform X11
XM_012970919.3	XP_012826373.1	kinesin-like protein KIF21B isoform X3
XM_012970929.2	XP_012826383.1	kinesin-like protein KIF21B isoform X5
XM_004910722.4	XP_004910779.1	kinesin-like protein KIF21B isoform X6
XM_004910721.3	XP_004910778.1	kinesin-like protein KIF21B isoform X2
XM_012970919.2	XP_012826373.1	kinesin-like protein KIF21B isoform X3
XM_012970929.1	XP_012826383.1	kinesin-like protein KIF21B isoform X5
XM_004910723.4	XP_004910780.1	kinesin-like protein KIF21B isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03842	XM_004910720.4 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa03843	XM_004910721.4 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa03844	XM_004910722.4 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa03845	XM_004910723.4 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa29025	XM_012970919.3 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa29026	XM_012970924.3 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa29027	XM_012970929.2 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa29028	XM_012970944.3 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa29030	XM_012970955.3 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa29029	XM_012970952.2 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29031	XM_012970961.2 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29032	XM_012970966.2 Latest version!	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OXa29026	XM_012970924.2	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29030	XM_012970955.2	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa03842	XM_004910720.3	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29028	XM_012970944.2	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa03844	XM_004910722.3	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa03843	XM_004910721.3	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29025	XM_012970919.2	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa29027	XM_012970929.1	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa03845	XM_004910723.3	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

kif21b ( XM_004910720.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910777.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03842
Clone ID Related Accession (Same CDS sequence)	XM_004910720.4 , XM_004910720.3
Accession Version	XM_004910720.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG 
GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT 
GCCACTAGCC TTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910777.1
CDS	185..5062
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910720.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910720.4

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG 
GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT 
GCCACTAGCC TTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_004910721.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910778.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.

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CloneID	OXa03843
Clone ID Related Accession (Same CDS sequence)	XM_004910721.4 , XM_004910721.3
Accession Version	XM_004910721.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910721.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910778.1
CDS	185..5059
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910721.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910721.4

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_004910722.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910779.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa03844
Clone ID Related Accession (Same CDS sequence)	XM_004910722.3 , XM_004910722.4
Accession Version	XM_004910722.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910722.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910779.1
CDS	185..5023
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910722.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910722.4

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_004910723.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910780.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.

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CloneID	OXa03845
Clone ID Related Accession (Same CDS sequence)	XM_004910723.3 , XM_004910723.4
Accession Version	XM_004910723.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910723.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA 
TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA 
CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA 
GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC 
CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA 
TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG 
TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA 
AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC 
CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT 
GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC 
GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT 
TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT 
ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC 
AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC 
TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT 
GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC 
GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC 
TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT 
CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910780.1
CDS	185..5020
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910723.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910723.4

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA 
TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA 
CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA 
GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC 
CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA 
TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG 
TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA 
AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC 
CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT 
GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC 
GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT 
TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT 
ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC 
AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC 
TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT 
GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC 
GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC 
TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT 
CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970919.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826373.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.

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CloneID	OXa29025
Clone ID Related Accession (Same CDS sequence)	XM_012970919.3 , XM_012970919.2
Accession Version	XM_012970919.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970919.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG 
GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA 
AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA 
AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC 
ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA 
CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT 
ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG 
ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA 
ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG 
ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG 
TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826373.1
CDS	185..5059
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970919.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970919.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG 
GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA 
AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA 
AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC 
ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA 
CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT 
ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG 
ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA 
ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG 
ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG 
TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970924.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826378.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.

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CloneID	OXa29026
Clone ID Related Accession (Same CDS sequence)	XM_012970924.3 , XM_012970924.2
Accession Version	XM_012970924.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT 
CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT 
GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT 
GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT 
ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT 
TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG 
CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT 
GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT 
CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC 
AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA 
AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG 
GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA 
TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT 
CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA 
CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA 
CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT 
ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT 
CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT 
ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG 
AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA 
CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA 
GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC 
TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC 
CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG 
CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC 
TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG 
AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT 
CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC 
ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT 
CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG 
TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC 
AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT 
AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT 
AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG 
GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC 
TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT 
TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT 
GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA 
CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC 
CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT 
GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC 
AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC 
AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG 
GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA 
ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA 
GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG 
GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG 
GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT 
GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG 
GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG 
CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA 
GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT 
CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA 
CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA 
TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT 
CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC 
TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG 
TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG 
CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG 
GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC 
TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT 
GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT 
GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT 
TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC 
CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT 
ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT 
GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG 
GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT 
GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA 
GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG 
TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT 
ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC 
ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT 
CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826378.1
CDS	185..5041
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKLYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970924.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970924.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT 
CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT 
GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT 
GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT 
ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT 
TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG 
CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT 
GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT 
CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC 
AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA 
AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG 
GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA 
TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT 
CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA 
CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA 
CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT 
ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT 
CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT 
ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG 
AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA 
CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA 
GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC 
TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC 
CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG 
CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC 
TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG 
AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT 
CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC 
ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT 
CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG 
TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC 
AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT 
AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT 
AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG 
GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC 
TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT 
TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT 
GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA 
CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC 
CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT 
GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC 
AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC 
AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG 
GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA 
ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA 
GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG 
GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG 
GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT 
GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG 
GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG 
CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA 
GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT 
CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA 
CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA 
TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT 
CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC 
TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG 
TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG 
CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG 
GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC 
TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT 
GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT 
GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT 
TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC 
CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT 
ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT 
GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG 
GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT 
GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA 
GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG 
TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT 
ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC 
ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT 
CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970929.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826383.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.

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CloneID	OXa29027
Clone ID Related Accession (Same CDS sequence)	XM_012970929.2 , XM_012970929.1
Accession Version	XM_012970929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG 
TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA 
GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA 
ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC 
CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG 
AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG 
GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT 
ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT 
GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA 
GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC 
CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT 
TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA 
GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG 
AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT 
TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT 
GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA 
AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA 
GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA 
GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA 
CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT 
ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT 
GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA 
CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC 
CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG 
GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA 
AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA 
GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA 
GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA 
GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT 
CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA 
TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA 
ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA 
CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA 
AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC 
AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG 
CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA 
CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG 
CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA 
GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG 
AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT 
CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA 
GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC 
AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG 
AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC 
ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT 
CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG 
CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG 
TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT 
GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA 
GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT 
CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC 
CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA 
CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT 
AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC 
TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA 
TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA 
AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT 
CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA 
TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC 
AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC 
CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA 
CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826383.1
CDS	132..4970
Translation	MIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970929.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970929.2

ORF Insert Sequence:

ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG 
TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA 
GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA 
ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC 
CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG 
AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG 
GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT 
ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT 
GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA 
GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC 
CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT 
TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA 
GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG 
AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT 
TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT 
GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA 
AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA 
GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA 
GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA 
CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT 
ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT 
GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA 
CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC 
CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG 
GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA 
AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA 
GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA 
GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA 
GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT 
CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA 
TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA 
ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA 
CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA 
AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC 
AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG 
CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA 
CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG 
CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA 
GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG 
AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT 
CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA 
GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC 
AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG 
AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC 
ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT 
CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG 
CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG 
TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT 
GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA 
GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT 
CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC 
CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA 
CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT 
AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC 
TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA 
TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA 
AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT 
CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA 
TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC 
AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC 
CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA 
CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970944.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826398.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.

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CloneID	OXa29028
Clone ID Related Accession (Same CDS sequence)	XM_012970944.3 , XM_012970944.2
Accession Version	XM_012970944.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG 
AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826398.1
CDS	186..5021
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDFTVKMWSRRGRSIVGIT

Target ORF information:

RefSeq Version	XM_012970944.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970944.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG 
AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970955.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826409.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa29030
Clone ID Related Accession (Same CDS sequence)	XM_012970955.3 , XM_012970955.2
Accession Version	XM_012970955.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012970955.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT 
GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT 
CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT 
CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG 
TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA 
ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA 
AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG 
AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA 
GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT 
CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC 
CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA 
AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA 
GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA 
GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC 
CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA 
GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG 
AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA 
GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG 
ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG 
ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT 
TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC 
CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT 
GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG 
CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT 
TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG 
TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT 
CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC 
CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT 
ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT 
GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA 
CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA 
AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA 
GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT 
TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA 
GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA 
GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC 
CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT 
GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC 
TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA 
ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT 
TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG 
TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT 
CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT 
AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT 
TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT 
CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT 
TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT 
ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC 
CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT 
CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826409.1
CDS	185..4927
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970955.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970955.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT 
GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT 
CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT 
CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG 
TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA 
ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA 
AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG 
AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA 
GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT 
CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC 
CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA 
AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA 
GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA 
GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC 
CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA 
GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG 
AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA 
GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG 
ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG 
ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT 
TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC 
CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT 
GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG 
CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT 
TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG 
TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT 
CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC 
CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT 
ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT 
GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA 
CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA 
AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA 
GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT 
TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA 
GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA 
GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC 
CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT 
GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC 
TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA 
ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT 
TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG 
TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT 
CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT 
AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT 
TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT 
CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT 
TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT 
ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC 
CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT 
CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_004910720.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910777.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.

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CloneID	OXa03842
Clone ID Related Accession (Same CDS sequence)	XM_004910720.4 , XM_004910720.3
Accession Version	XM_004910720.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG 
GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT 
GCCACTAGCC TTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910777.1
CDS	190..5067
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910720.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910720.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTGCCGAGT CAAGCTATGG 
GTTTATGTTC CTGGTCTTAC TCCATGTCTC CCTCGACGTG TTCTGGCAAT CAAGGGCCGT 
GCCACTAGCC TTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_004910721.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910778.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.

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CloneID	OXa03843
Clone ID Related Accession (Same CDS sequence)	XM_004910721.4 , XM_004910721.3
Accession Version	XM_004910721.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910778.1
CDS	190..5064
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910721.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910721.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_004910722.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910779.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.

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CloneID	OXa03844
Clone ID Related Accession (Same CDS sequence)	XM_004910722.3 , XM_004910722.4
Accession Version	XM_004910722.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910779.1
CDS	190..5028
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910722.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910722.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_004910723.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910780.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.

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CloneID	OXa03845
Clone ID Related Accession (Same CDS sequence)	XM_004910723.3 , XM_004910723.4
Accession Version	XM_004910723.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA 
TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA 
CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA 
GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC 
CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA 
TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG 
TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA 
AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC 
CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT 
GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC 
GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT 
TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT 
ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC 
AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC 
TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT 
GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC 
GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC 
TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT 
CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910780.1
CDS	190..5025
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_004910723.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910723.3

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGGGCAT AATAAGTCAA 
TTTGGAGGGC TAAAGAGCAT GAGGACAGCT CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA 
CATACAAAGC CAGTTCTTTG TGTTGAAGCT ACAGATGAGC TCCTCTTTAC AGGATCTAAA 
GATCGCACAT GCAAACTCTG GAATCTGGTA ACAGGGCAGG AAATTGCATC CCTTCGCAAC 
CATCCCCATA ACGTTGTATC TCTGAAACAT TGCCCAAACT CTGGGCTAGT TCTCACAGCA 
TCTGCTTCTT GTGTGCGAGT ATGGGATATC CGTGACAGTG CTCGCTGTGT GAGGACATTG 
TTGTCATCAG GCCAGGTAAT GTCAGGAGAT ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA 
AGCAGCACTC AGGCAGAGTT GCAAATTAAT CAAATAACTT TAAATCCTGA TGGCACAATC 
CTCTATGCAG CAGCCGGCAA TGCAGTGAGA GCTTGGGACC TAAGCAGGTT TCAACCCATT 
GGAAAGCTAA CAGGACACAT GGCTCCAGTC ATGTGTCTTA CAGTGAGCAA CACACAAAAC 
GGACGAGATC TTGTTATCAC AGGATCTAAA GATCACTATG TCAAGATATT TGAACTCCAT 
TCTGGATGGG GAGGGGCAGT TCCCCCCACT TATAACCTGG AACCTCCTCA TTACGATGGT 
ATTGAGTGCT TGGCTGTACA GAATGGGATC CTTTGCAGTG GCTCTCGAGA TAATGGAATC 
AAAAAGTGGG ATCTACAGCA ACATGAGCTC ACTCAGCAAG TGCCAAATGC ACACAGAGAC 
TGGATTTGTG CTTTAGGATT TGTACCGGGA CGTTCAATGT TGCTTAGTGG ATGTCGTGGT 
GGCATTCTAA AGGTTTGGAA CATGGAGAAC TTTACACCTA TTGGGGAGAT CAAAGGCCAC 
GAAAGCCCCA TTAATGCGAT GTGTTCTAAT AGCCGTCACA TCTTCACTGC ATCTAGTGAC 
TGCCGAGTCA AGCTATGGGT TTATGTTCCT GGTCTTACTC CATGTCTCCC TCGACGTGTT 
CTGGCAATCA AGGGCCGTGC CACTAGCCTT CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970919.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826373.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.

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CloneID	OXa29025
Clone ID Related Accession (Same CDS sequence)	XM_012970919.3 , XM_012970919.2
Accession Version	XM_012970919.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG 
GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA 
AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA 
AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC 
ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA 
CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT 
ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG 
ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA 
ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG 
ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG 
TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826373.1
CDS	190..5064
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970919.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970919.2

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGGAAAGGG 
GTACGTCCAG GCCTACCACT CAGTAAGGCT GCTCACATGA AGTGGCAAAC TCTGGAGAGA 
AGGGTTCATG AAGTCGTCCT ACAGAGACTT ACGCTTCTTA ACTTAGAAAG CGATATGGAA 
AGACTGTTGC GGAAACGTGA GGAGCTGTCA CTTCTGCAGG AAGCTTTGAT AAGTCGTTCC 
ATAGCTCTCC CAGAAGACAG GAAAGCAGAG GAAAAGTCAC AGCAGGATGT TGCCGAAGAA 
CTGGAAGCTT TGACAGCCAA TATTGATTAT GTTAATGACT GCATTCGAGA ATGCCAGGCT 
ACCATTGTTC AGATGGAGGA AACAAAGGAG GATCTAGACT CAACAGACAC CTCAGTTGTG 
ATCAGTTCTT GTTCTCTAAC TGAGGCACGA CATCTTCTTG ATAATTTCCT GAGAGCCTCA 
ATAGATAAGG GCCTGCAAGT AGCTCAGAAA GAAGCCCAGA TACGGATTCT GGAAGCCCGG 
ATTCGGCAAA CTGACATGGC TGGCCTGTCA CAGGCACACA TGCTGCTGGA GAAATCAGAG 
TGCCATTCTG TGCAGCAGGC AGAAAACGGG TATGCTAGCA CAGATGAAGA GGTGTCAGAA 
ATTAGCCAGA CTTCTGATGG AAGTGCTTCT TTCTCCTCCA TGAAAGGATC CTGCAGTCAA 
GATGACTTTA AGTGGAAGGG GGAACCACGG CTTTCATGGC AAGTGAAGGC CGTGTCTGCT 
GAGTCTTTGA GTCCGCTGCT TGAAGCTTCC ACTAGAAACA TCACCAAATC CTTAGCATCA 
TTAACTGACA TCCTAGAAGA TGTTGGGGGA TTCTCTCCGA GTGGTTTGCG GGGCAGAGCA 
TCACACAGCC TTACTCTTCC AATACGTGGT AATACATTTC CGAGACAATC CAGAGGTTTC 
AACACATCCC CTGTGACACG CAGAAAATCA TATGACAGAG GACAGCCAAG GGCTTCAGAC 
TCAAACCTAA CACCTCCATC ATCTCCCCCT ATGCGCCCTC GTAATGATAA AAATGTCTTT 
TCTCGGCTAA CAAGCAGTCA GAGTTCAGGC ACTGCACTGG ATAAGTCTGA TGAGAGTGAC 
AGCTCTTTCT CAGATGTCTC CAGGGGCATA ATAAGTCAAT TTGGAGGGCT AAAGAGCATG 
AGGACAGCTC CTTTGCAGTG TGTTGCAATT GCTGAGGGAC ATACAAAGCC AGTTCTTTGT 
GTTGAAGCTA CAGATGAGCT CCTCTTTACA GGATCTAAAG ATCGCACATG CAAACTCTGG 
AATCTGGTAA CAGGGCAGGA AATTGCATCC CTTCGCAACC ATCCCCATAA CGTTGTATCT 
CTGAAACATT GCCCAAACTC TGGGCTAGTT CTCACAGCAT CTGCTTCTTG TGTGCGAGTA 
TGGGATATCC GTGACAGTGC TCGCTGTGTG AGGACATTGT TGTCATCAGG CCAGGTAATG 
TCAGGAGATA TCTGCTCTGG GCTAGGTTTC CGCTCAGGAA GCAGCACTCA GGCAGAGTTG 
CAAATTAATC AAATAACTTT AAATCCTGAT GGCACAATCC TCTATGCAGC AGCCGGCAAT 
GCAGTGAGAG CTTGGGACCT AAGCAGGTTT CAACCCATTG GAAAGCTAAC AGGACACATG 
GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC ACACAAAACG GACGAGATCT TGTTATCACA 
GGATCTAAAG ATCACTATGT CAAGATATTT GAACTCCATT CTGGATGGGG AGGGGCAGTT 
CCCCCCACTT ATAACCTGGA ACCTCCTCAT TACGATGGTA TTGAGTGCTT GGCTGTACAG 
AATGGGATCC TTTGCAGTGG CTCTCGAGAT AATGGAATCA AAAAGTGGGA TCTACAGCAA 
CATGAGCTCA CTCAGCAAGT GCCAAATGCA CACAGAGACT GGATTTGTGC TTTAGGATTT 
GTACCGGGAC GTTCAATGTT GCTTAGTGGA TGTCGTGGTG GCATTCTAAA GGTTTGGAAC 
ATGGAGAACT TTACACCTAT TGGGGAGATC AAAGGCCACG AAAGCCCCAT TAATGCGATG 
TGTTCTAATA GCCGTCACAT CTTCACTGCA TCTAGTGACT GCCGAGTCAA GCTATGGGTT 
TATGTTCCTG GTCTTACTCC ATGTCTCCCT CGACGTGTTC TGGCAATCAA GGGCCGTGCC 
ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970924.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826378.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa29026
Clone ID Related Accession (Same CDS sequence)	XM_012970924.3 , XM_012970924.2
Accession Version	XM_012970924.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT 
CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT 
GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT 
GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT 
ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT 
TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG 
CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT 
GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT 
CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC 
AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA 
AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG 
GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA 
TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT 
CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA 
CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA 
CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT 
ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT 
CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT 
ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG 
AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA 
CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA 
GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC 
TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC 
CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG 
CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC 
TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG 
AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT 
CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC 
ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT 
CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG 
TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC 
AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT 
AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT 
AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG 
GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC 
TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT 
TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT 
GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA 
CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC 
CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT 
GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC 
AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC 
AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG 
GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA 
ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA 
GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG 
GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG 
GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT 
GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG 
GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG 
CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA 
GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT 
CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA 
CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA 
TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT 
CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC 
TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG 
TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG 
CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG 
GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC 
TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT 
GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT 
GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT 
TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC 
CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT 
ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT 
GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG 
GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT 
GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA 
GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG 
TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT 
ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC 
ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT 
CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826378.1
CDS	190..5046
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKLYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970924.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970924.2

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAACTCT ATAATGAGGA AATCTTGGAT CTATTTGATG TATCCCGTGA TCCTGACTCT 
CGCTATCGGA AATCTAATAT TCGGATCCAC GAGGATGCTA GCGGAGGGAT CTATGTTAGT 
GGTGCCAGCA TTCGCAGTGT CAGCTCAGAA GGAGAGCTAC TACAGCTTCT AAAGGAGGGT 
GCTCTTTCTA GAACTACAGC CAGTACCCAG ATGAATTCCC AGAGTTCACG GTCACATGCT 
ATCTTCTCAG TCCATCTCCA ACAAACACGT GTTTCTGAGA CTTGCTCGAA GGACAGTTCT 
TCTTTGGAGT ATGAGACTTT GACTGCTAAA TTTCACTTTG TGGACCTGGC AGGATCTGAG 
CGCCTCAAGC GTACAGGAGC AACTGGAGAG AGAGCAAGAG AAGGGATCTC TATAAATTGT 
GGCCTTCTGG CACTTGGGAA TGTAATCAGT GCTCTTGGAG ATCAAAGTAA GAAGGTTCTT 
CATGTTCCAT ACAGAGATTC AAAACTGACT CGACTACTGC AGGATTCATT AGGTGGTAAC 
AGTCAAACCA TGATGATTGC TTGCGTTTCT CCATCTGATC GTGATTTCAT GGAAACCTTA 
AACACTCTAA AGTATGCAAA TCGTGCACGA AACATTAAGA ATCGTGTAAC AGCCAATCAG 
GAGCAGAGTG GACAGCAAGT ACAGGCTCTT CGGGCAGAAC TAGCCAGAGT AAAGTTGGAA 
TTACAAGAGT ACAAGACAGG TCATCGAGTT GCTGGTAATG ATAGCTTAAG CGATATGTGT 
CAGGAGCTGT CTATGTTACG TAGTGAGAAT GCATCATTGC GGCTTCGTGT TACTGCCCTA 
CAAGAGGCTA TTAGAGCTAT CAATGCTCGT GTCACTCGTA TGGCTGGACA AGAAGCTGCA 
CACTTGGTGC AGCGAGCTGG TGATAGTAAT GAAGAAATAC AAACTCTCAT ACAGAACTAT 
ATCCATGAAA TAGAAGAACT CAGAACCCGT CTGGTAGAAA GTGAAGCAAT GAATGAATCT 
CTCAGACGCA GTTTGTCCCG TTCAACTGCA CGCTCATTGG TAACAGGACC TGCAACCCCT 
ACCCCATCAT TAGAAAAGGA GGCCAATGAA GTTGTTCGAA GAGCAAAAGA AGAAGTACAG 
AATTTGAAAA AGAAAGGAAG TTCATTACAG TCTGATAACT ACAAAAAGAG CTTGAAACAA 
CAACTGCAAA TCAGTGAAGA GACAGATTAT AATGAAGGAG AAGAGGAGGA TGACCATGAA 
GAAAGTGAAT GCGATGAAGA GGAAAGTGAG GGAAGTGGGG AGAGTGAGGC AGAGTCAGAC 
TCAGAACCCG AAGAGAAAGG GTTGGGCTGT TGGGACGCAG GGAAAAAATC CAGTGGGCCC 
CTGTCCTTGT GGGCCCCTCT GACCCAAGCC GGATCCCTGC AAGCGCCCCC ACCCAGTCAG 
CCCTCACCCA GTTTGCCATT CTGCATGGAG GAAGGCTCAC TTGGAGGAGA CCTTGCTGAC 
TTGACTTGTG AGATTGAAAT TAAGCAGCGT CTGATTGAGG AGTTAGAGCA AAGTCAGCGG 
AGACTACAAA CACTCAAACT TCAGTATGAG GAAAAATTGC AGCAACTCCA GAGTCGGATT 
CGTGATACAC AGCTGGAAAG GGACCGAGTG CTACGGGAAC TTAGCTCCAT GGAGTGTTAC 
ACAGAGGAAA AGGCTAACAA GATCCGTGCT GATTATGAGA AAAAGCTTCG GGAAATGAAT 
CGAGACTTGC AGAAGCTGCA GTTGGCTCAG AGAGAGCATA CACGACTCCT TAAAAATCAG 
TCCCGTTATG AACGTGAACT TAAGAAATTG CAGATGGAAC TAAGTGATAT GAAGAAGGCC 
AAATCAGCTC TAATGAAGCA GATGCGGGAA GAACAGCAGC TCAGAAAACT TGCAGAATCT 
AGAAGGAACA AAGAAATAGC TCAGCTGAAG AAGGAGCACC ATAGACAAGA GCTTCACATT 
AGAGCTTTGG AGTCCCAGAA GAGACAGCAA GAGATAGTGC TGAGACGCAA GACCCAAGAG 
GTTTCTGCTC TGCGGCGTCT CTCTAGGCGA CAAACTGATC GATCTGGGGG ACGAAGCCAC 
TCTGTTGCAG ACTCTGGAGC AGAGTTGTCC ACAAGCACAG CATCTTCAGA ACCAGATAGT 
TCTTTACGTT CTGTGTCCAG TATTGTCCGT CATTGGAACC GAAAGACAAG TGGAGACGTT 
GGGCCAGTGC CCAATGGGAG TCGTACACCA AGAAGGAAAG GGGTACGTCC AGGCCTACCA 
CTCAGTAAGG CTGCTCACAT GAAGTGGCAA ACTCTGGAGA GAAGGGTTCA TGAAGTCGTC 
CTACAGAGAC TTACGCTTCT TAACTTAGAA AGCGATATGG AAAGACTGTT GCGGAAACGT 
GAGGAGCTGT CACTTCTGCA GGAAGCTTTG ATAAGTCGTT CCATAGCTCT CCCAGAAGAC 
AGGAAAGCAG AGGAAAAGTC ACAGCAGGAT GTTGCCGAAG AACTGGAAGC TTTGACAGCC 
AATATTGATT ATGTTAATGA CTGCATTCGA GAATGCCAGG CTACCATTGT TCAGATGGAG 
GAAACAAAGG AGGATCTAGA CTCAACAGAC ACCTCAGTTG TGATCAGTTC TTGTTCTCTA 
ACTGAGGCAC GACATCTTCT TGATAATTTC CTGAGAGCCT CAATAGATAA GGGCCTGCAA 
GTAGCTCAGA AAGAAGCCCA GATACGGATT CTGGAAGCCC GGATTCGGCA AACTGACATG 
GCTGGCCTGT CACAGGCACA CATGCTGCTG GAGAAATCAG AGTGCCATTC TGTGCAGCAG 
GCAGAAAACG GGTATGCTAG CACAGATGAA GAGGTGTCAG AAATTAGCCA GACTTCTGAT 
GGAAGTGCTT CTTTCTCCTC CATGAAAGGA TCCTGCAGTC AAGATGACTT TAAGTGGAAG 
GGGGAACCAC GGCTTTCATG GCAAGTGAAG GCCGTGTCTG CTGAGTCTTT GAGTCCGCTG 
CTTGAAGCTT CCACTAGAAA CATCACCAAA TCCTTAGCAT CATTAACTGA CATCCTAGAA 
GATGTTGGGG GATTCTCTCC GAGTGGTTTG CGGGGCAGAG CATCACACAG CCTTACTCTT 
CCAATACGTG GTAATACATT TCCGAGACAA TCCAGAGGTT TCAACACATC CCCTGTGACA 
CGCAGAAAAT CATATGACAG AGGACAGCCA AGGGCTTCAG ACTCAAACCT AACACCTCCA 
TCATCTCCCC CTATGCGCCC TCGTAATGAT AAAAATGTCT TTTCTCGGCT AACAAGCAGT 
CAGAGTTCAG GCACTGCACT GGATAAGTCT GATGAGAGTG ACAGCTCTTT CTCAGATGTC 
TCCAGGGGCA TAATAAGTCA ATTTGGAGGG CTAAAGAGCA TGAGGACAGC TCCTTTGCAG 
TGTGTTGCAA TTGCTGAGGG ACATACAAAG CCAGTTCTTT GTGTTGAAGC TACAGATGAG 
CTCCTCTTTA CAGGATCTAA AGATCGCACA TGCAAACTCT GGAATCTGGT AACAGGGCAG 
GAAATTGCAT CCCTTCGCAA CCATCCCCAT AACGTTGTAT CTCTGAAACA TTGCCCAAAC 
TCTGGGCTAG TTCTCACAGC ATCTGCTTCT TGTGTGCGAG TATGGGATAT CCGTGACAGT 
GCTCGCTGTG TGAGGACATT GTTGTCATCA GGCCAGGTAA TGTCAGGAGA TATCTGCTCT 
GGGCTAGGTT TCCGCTCAGG AAGCAGCACT CAGGCAGAGT TGCAAATTAA TCAAATAACT 
TTAAATCCTG ATGGCACAAT CCTCTATGCA GCAGCCGGCA ATGCAGTGAG AGCTTGGGAC 
CTAAGCAGGT TTCAACCCAT TGGAAAGCTA ACAGGACACA TGGCTCCAGT CATGTGTCTT 
ACAGTGAGCA ACACACAAAA CGGACGAGAT CTTGTTATCA CAGGATCTAA AGATCACTAT 
GTCAAGATAT TTGAACTCCA TTCTGGATGG GGAGGGGCAG TTCCCCCCAC TTATAACCTG 
GAACCTCCTC ATTACGATGG TATTGAGTGC TTGGCTGTAC AGAATGGGAT CCTTTGCAGT 
GGCTCTCGAG ATAATGGAAT CAAAAAGTGG GATCTACAGC AACATGAGCT CACTCAGCAA 
GTGCCAAATG CACACAGAGA CTGGATTTGT GCTTTAGGAT TTGTACCGGG ACGTTCAATG 
TTGCTTAGTG GATGTCGTGG TGGCATTCTA AAGGTTTGGA ACATGGAGAA CTTTACACCT 
ATTGGGGAGA TCAAAGGCCA CGAAAGCCCC ATTAATGCGA TGTGTTCTAA TAGCCGTCAC 
ATCTTCACTG CATCTAGTGA CTGCCGAGTC AAGCTATGGG TTTATGTTCC TGGTCTTACT 
CCATGTCTCC CTCGACGTGT TCTGGCAATC AAGGGCCGTG CCACTAGCCT TCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970929.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826383.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.

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CloneID	OXa29027
Clone ID Related Accession (Same CDS sequence)	XM_012970929.2 , XM_012970929.1
Accession Version	XM_012970929.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG 
TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA 
GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA 
ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC 
CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG 
AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG 
GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT 
ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT 
GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA 
GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC 
CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT 
TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA 
GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG 
AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT 
TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT 
GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA 
AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA 
GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA 
GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA 
CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT 
ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT 
GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA 
CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC 
CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG 
GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA 
AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA 
GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA 
GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA 
GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT 
CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA 
TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA 
ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA 
CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA 
AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC 
AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG 
CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA 
CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG 
CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA 
GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG 
AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT 
CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA 
GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC 
AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG 
AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC 
ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT 
CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG 
CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG 
TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT 
GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA 
GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT 
CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC 
CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA 
CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT 
AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC 
TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA 
TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA 
AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT 
CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA 
TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC 
AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC 
CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA 
CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826383.1
CDS	146..4984
Translation	MIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970929.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970929.1

ORF Insert Sequence:

ATGATTCGAC CACAGAGCAG TAAGGAGCAG ACTGAAGGAT GCCGTATATG TACATGGGTT 
TATGCTGGAG AGCCACAGGT GCTTTTGGGT AAAGACAAAG CCTTCACATA TGATTTTGTG 
TTTGATATGG ACACAGACCA AATCCAAGTC TATGAGACTT GTGTACGTGG GCTGGTAGAA 
GGTTGTTTTC AAGGCTACAA TGCTACTGTG CTGGCCTATG GACAGACTGG TGCTGGTAAA 
ACACATACAA TGGGTAGTGG ATTTGATCTT GTGGTAACTG AGGAAGAACT GGGGATCATC 
CCACGTGCTG TGCGTCAGCT TTTCAGCACC ATACAAGAGC ACAAAGCTTC TGCTTATGAG 
AGAGGACTGC CAGAGCCCAG CTTTAAAGTT AGTGCACAAT TCTTAGAACT CTATAATGAG 
GAAATCTTGG ATCTATTTGA TGTATCCCGT GATCCTGACT CTCGCTATCG GAAATCTAAT 
ATTCGGATCC ACGAGGATGC TAGCGGAGGG ATCTATGTTA GTGGTGCCAG CATTCGCAGT 
GTCAGCTCAG AAGGAGAGCT ACTACAGCTT CTAAAGGAGG GTGCTCTTTC TAGAACTACA 
GCCAGTACCC AGATGAATTC CCAGAGTTCA CGGTCACATG CTATCTTCTC AGTCCATCTC 
CAACAAACAC GTGTTTCTGA GACTTGCTCG AAGGACAGTT CTTCTTTGGA GTATGAGACT 
TTGACTGCTA AATTTCACTT TGTGGACCTG GCAGGATCTG AGCGCCTCAA GCGTACAGGA 
GCAACTGGAG AGAGAGCAAG AGAAGGGATC TCTATAAATT GTGGCCTTCT GGCACTTGGG 
AATGTAATCA GTGCTCTTGG AGATCAAAGT AAGAAGGTTC TTCATGTTCC ATACAGAGAT 
TCAAAACTGA CTCGACTACT GCAGGATTCA TTAGGTGGTA ACAGTCAAAC CATGATGATT 
GCTTGCGTTT CTCCATCTGA TCGTGATTTC ATGGAAACCT TAAACACTCT AAAGTATGCA 
AATCGTGCAC GAAACATTAA GAATCGTGTA ACAGCCAATC AGGAGCAGAG TGGACAGCAA 
GTACAGGCTC TTCGGGCAGA ACTAGCCAGA GTAAAGTTGG AATTACAAGA GTACAAGACA 
GGTCATCGAG TTGCTGGTAA TGATAGCTTA AGCGATATGT GTCAGGAGCT GTCTATGTTA 
CGTAGTGAGA ATGCATCATT GCGGCTTCGT GTTACTGCCC TACAAGAGGC TATTAGAGCT 
ATCAATGCTC GTGTCACTCG TATGGCTGGA CAAGAAGCTG CACACTTGGT GCAGCGAGCT 
GGTGATAGTA ATGAAGAAAT ACAAACTCTC ATACAGAACT ATATCCATGA AATAGAAGAA 
CTCAGAACCC GTCTGGTAGA AAGTGAAGCA ATGAATGAAT CTCTCAGACG CAGTTTGTCC 
CGTTCAACTG CACGCTCATT GGTAACAGGA CCTGCAACCC CTACCCCATC ATTAGAAAAG 
GAGGCCAATG AAGTTGTTCG AAGAGCAAAA GAAGAAGTAC AGAATTTGAA AAAGAAAGGA 
AGTTCATTAC AGTCTGATAA CTACAAAAAG AGCTTGAAAC AACAACTGCA AATCAGTGAA 
GAGACAGATT ATAATGAAGG AGAAGAGGAG GATGACCATG AAGAAAGTGA ATGCGATGAA 
GAGGAAAGTG AGGGAAGTGG GGAGAGTGAG GCAGAGTCAG ACTCAGAACC CGAAGAGAAA 
GGGTTGGGCT GTTGGGACGC AGGGAAAAAA TCCAGTGGGC CCCTGTCCTT GTGGGCCCCT 
CTGACCCAAG CCGGATCCCT GCAAGCGCCC CCACCCAGTC AGCCCTCACC CAGTTTGCCA 
TTCTGCATGG AGGAAGGCTC ACTTGGAGGA GACCTTGCTG ACTTGACTTG TGAGATTGAA 
ATTAAGCAGC GTCTGATTGA GGAGTTAGAG CAAAGTCAGC GGAGACTACA AACACTCAAA 
CTTCAGTATG AGGAAAAATT GCAGCAACTC CAGAGTCGGA TTCGTGATAC ACAGCTGGAA 
AGGGACCGAG TGCTACGGGA ACTTAGCTCC ATGGAGTGTT ACACAGAGGA AAAGGCTAAC 
AAGATCCGTG CTGATTATGA GAAAAAGCTT CGGGAAATGA ATCGAGACTT GCAGAAGCTG 
CAGTTGGCTC AGAGAGAGCA TACACGACTC CTTAAAAATC AGTCCCGTTA TGAACGTGAA 
CTTAAGAAAT TGCAGATGGA ACTAAGTGAT ATGAAGAAGG CCAAATCAGC TCTAATGAAG 
CAGATGCGGG AAGAACAGCA GCTCAGAAAA CTTGCAGAAT CTAGAAGGAA CAAAGAAATA 
GCTCAGCTGA AGAAGGAGCA CCATAGACAA GAGCTTCACA TTAGAGCTTT GGAGTCCCAG 
AAGAGACAGC AAGAGATAGT GCTGAGACGC AAGACCCAAG AGGTTTCTGC TCTGCGGCGT 
CTCTCTAGGC GACAAACTGA TCGATCTGGG GGACGAAGCC ACTCTGTTGC AGACTCTGGA 
GCAGAGTTGT CCACAAGCAC AGCATCTTCA GAACCAGATA GTTCTTTACG TTCTGTGTCC 
AGTATTGTCC GTCATTGGAA CCGAAAGACA AGTGGAGACG TTGGGCCAGT GCCCAATGGG 
AGTCGTACAC CAAGAAGGAA AGGGGTACGT CCAGGCCTAC CACTCAGTAA GGCTGCTCAC 
ATGAAGTGGC AAACTCTGGA GAGAAGGGTT CATGAAGTCG TCCTACAGAG ACTTACGCTT 
CTTAACTTAG AAAGCGATAT GGAAAGACTG TTGCGGAAAC GTGAGGAGCT GTCACTTCTG 
CAGGAAGCTT TGATAAGTCG TTCCATAGCT CTCCCAGAAG ACAGGAAAGC AGAGGAAAAG 
TCACAGCAGG ATGTTGCCGA AGAACTGGAA GCTTTGACAG CCAATATTGA TTATGTTAAT 
GACTGCATTC GAGAATGCCA GGCTACCATT GTTCAGATGG AGGAAACAAA GGAGGATCTA 
GACTCAACAG ACACCTCAGT TGTGATCAGT TCTTGTTCTC TAACTGAGGC ACGACATCTT 
CTTGATAATT TCCTGAGAGC CTCAATAGAT AAGGGCCTGC AAGTAGCTCA GAAAGAAGCC 
CAGATACGGA TTCTGGAAGC CCGGATTCGG CAAACTGACA TGGCTGGCCT GTCACAGGCA 
CACATGCTGC TGGAGAAATC AGAGTGCCAT TCTGTGCAGC AGGCAGAAAA CGGGTATGCT 
AGCACAGATG AAGAGGTGTC AGAAATTAGC CAGACTTCTG ATGGAAGTGC TTCTTTCTCC 
TCCATGAAAG GATCCTGCAG TCAAGATGAC TTTAAGTGGA AGGGGGAACC ACGGCTTTCA 
TGGCAAGTGA AGGCCGTGTC TGCTGAGTCT TTGAGTCCGC TGCTTGAAGC TTCCACTAGA 
AACATCACCA AATCCTTAGC ATCATTAACT GACATCCTAG AAGATGTTGG GGGATTCTCT 
CCGAGTGGTT TGCGGGGCAG AGCATCACAC AGCCTTACTC TTCCAATACG TGGTAATACA 
TTTCCGAGAC AATCCAGAGG TTTCAACACA TCCCCTGTGA CACGCAGAAA ATCATATGAC 
AGAGGACAGC CAAGGGCTTC AGACTCAAAC CTAACACCTC CATCATCTCC CCCTATGCGC 
CCTCGTAATG ATAAAAATGT CTTTTCTCGG CTAACAAGCA GTCAGAGTTC AGGCACTGCA 
CTGGATAAGT CTGATGAGAG TGACAGCTCT TTCTCAGATG TCTCCAGGGG CATAATAAGT 
CAATTTGGAG GGCTAAAGAG CATGAGGACA GCTCCTTTGC AGTGTGTTGC AATTGCTGAG 
GGACATACAA AGCCAGTTCT TTGTGTTGAA GCTACAGATG AGCTCCTCTT TACAGGATCT 
AAAGATCGCA CATGCAAACT CTGGAATCTG GTAACAGGGC AGGAAATTGC ATCCCTTCGC 
AACCATCCCC ATAACGTTGT ATCTCTGAAA CATTGCCCAA ACTCTGGGCT AGTTCTCACA 
GCATCTGCTT CTTGTGTGCG AGTATGGGAT ATCCGTGACA GTGCTCGCTG TGTGAGGACA 
TTGTTGTCAT CAGGCCAGGT AATGTCAGGA GATATCTGCT CTGGGCTAGG TTTCCGCTCA 
GGAAGCAGCA CTCAGGCAGA GTTGCAAATT AATCAAATAA CTTTAAATCC TGATGGCACA 
ATCCTCTATG CAGCAGCCGG CAATGCAGTG AGAGCTTGGG ACCTAAGCAG GTTTCAACCC 
ATTGGAAAGC TAACAGGACA CATGGCTCCA GTCATGTGTC TTACAGTGAG CAACACACAA 
AACGGACGAG ATCTTGTTAT CACAGGATCT AAAGATCACT ATGTCAAGAT ATTTGAACTC 
CATTCTGGAT GGGGAGGGGC AGTTCCCCCC ACTTATAACC TGGAACCTCC TCATTACGAT 
GGTATTGAGT GCTTGGCTGT ACAGAATGGG ATCCTTTGCA GTGGCTCTCG AGATAATGGA 
ATCAAAAAGT GGGATCTACA GCAACATGAG CTCACTCAGC AAGTGCCAAA TGCACACAGA 
GACTGGATTT GTGCTTTAGG ATTTGTACCG GGACGTTCAA TGTTGCTTAG TGGATGTCGT 
GGTGGCATTC TAAAGGTTTG GAACATGGAG AACTTTACAC CTATTGGGGA GATCAAAGGC 
CACGAAAGCC CCATTAATGC GATGTGTTCT AATAGCCGTC ACATCTTCAC TGCATCTAGT 
GACTGCCGAG TCAAGCTATG GGTTTATGTT CCTGGTCTTA CTCCATGTCT CCCTCGACGT 
GTTCTGGCAA TCAAGGGCCG TGCCACTAGC CTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970944.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826398.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.

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CloneID	OXa29028
Clone ID Related Accession (Same CDS sequence)	XM_012970944.3 , XM_012970944.2
Accession Version	XM_012970944.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG 
AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826398.1
CDS	190..5025
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDFTVKMWSRRGRSIVGIT

Target ORF information:

RefSeq Version	XM_012970944.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970944.2

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTCATC AGGCCAGGTA 
ATGTCAGGAG ATATCTGCTC TGGGCTAGGT TTCCGCTCAG GAAGCAGCAC TCAGGCAGAG 
TTGCAAATTA ATCAAATAAC TTTAAATCCT GATGGCACAA TCCTCTATGC AGCAGCCGGC 
AATGCAGTGA GAGCTTGGGA CCTAAGCAGG TTTCAACCCA TTGGAAAGCT AACAGGACAC 
ATGGCTCCAG TCATGTGTCT TACAGTGAGC AACACACAAA ACGGACGAGA TCTTGTTATC 
ACAGGATCTA AAGATCACTA TGTCAAGATA TTTGAACTCC ATTCTGGATG GGGAGGGGCA 
GTTCCCCCCA CTTATAACCT GGAACCTCCT CATTACGATG GTATTGAGTG CTTGGCTGTA 
CAGAATGGGA TCCTTTGCAG TGGCTCTCGA GATAATGGAA TCAAAAAGTG GGATCTACAG 
CAACATGAGC TCACTCAGCA AGTGCCAAAT GCACACAGAG ACTGGATTTG TGCTTTAGGA 
TTTGTACCGG GACGTTCAAT GTTGCTTAGT GGATGTCGTG GTGGCATTCT AAAGGTTTGG 
AACATGGAGA ACTTTACACC TATTGGGGAG ATCAAAGGCC ACGAAAGCCC CATTAATGCG 
ATGTGTTCTA ATAGCCGTCA CATCTTCACT GCATCTAGTG ACTTCACAGT GAAAATGTGG 
AGTAGAAGAG GAAGAAGTAT TGTTGGCATT ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970952.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826406.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.

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CloneID	OXa29029
Clone ID Related Accession (Same CDS sequence)	XM_012970952.2
Accession Version	XM_012970952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGAAG 
GACAGTTCTT CTTTGGAGTA TGAGACTTTG ACTGCTAAAT TTCACTTTGT GGACCTGGCA 
GGATCTGAGC GCCTCAAGCG TACAGGAGCA ACTGGAGAGA GAGCAAGAGA AGGGATCTCT 
ATAAATTGTG GCCTTCTGGC ACTTGGGAAT GTAATCAGTG CTCTTGGAGA TCAAAGTAAG 
AAGGTTCTTC ATGTTCCATA CAGAGATTCA AAACTGACTC GACTACTGCA GGATTCATTA 
GGTGGTAACA GTCAAACCAT GATGATTGCT TGCGTTTCTC CATCTGATCG TGATTTCATG 
GAAACCTTAA ACACTCTAAA GTATGCAAAT CGTGCACGAA ACATTAAGAA TCGTGTAACA 
GCCAATCAGG AGCAGAGTGG ACAGCAAGTA CAGGCTCTTC GGGCAGAACT AGCCAGAGTA 
AAGTTGGAAT TACAAGAGTA CAAGACAGGT CATCGAGTTG CTGGTAATGA TAGCTTAAGC 
GATATGTGTC AGGAGCTGTC TATGTTACGT AGTGAGAATG CATCATTGCG GCTTCGTGTT 
ACTGCCCTAC AAGAGGCTAT TAGAGCTATC AATGCTCGTG TCACTCGTAT GGCTGGACAA 
GAAGCTGCAC ACTTGGTGCA GCGAGCTGGT GATAGTAATG AAGAAATACA AACTCTCATA 
CAGAACTATA TCCATGAAAT AGAAGAACTC AGAACCCGTC TGGTAGAAAG TGAAGCAATG 
AATGAATCTC TCAGACGCAG TTTGTCCCGT TCAACTGCAC GCTCATTGGT AACAGGACCT 
GCAACCCCTA CCCCATCATT AGAAAAGGAG GCCAATGAAG TTGTTCGAAG AGCAAAAGAA 
GAAGTACAGA ATTTGAAAAA GAAAGGAAGT TCATTACAGT CTGATAACTA CAAAAAGAGC 
TTGAAACAAC AACTGCAAAT CAGTGAAGAG ACAGATTATA ATGAAGGAGA AGAGGAGGAT 
GACCATGAAG AAAGTGAATG CGATGAAGAG GAAAGTGAGG GAAGTGGGGA GAGTGAGGCA 
GAGTCAGACT CAGAACCCGA AGAGAAAGGG TTGGGCTGTT GGGACGCAGG GAAAAAATCC 
AGTGGGCCCC TGTCCTTGTG GGCCCCTCTG ACCCAAGCCG GATCCCTGCA AGCGCCCCCA 
CCCAGTCAGC CCTCACCCAG TTTGCCATTC TGCATGGAGG AAGGCTCACT TGGAGGAGAC 
CTTGCTGACT TGACTTGTGA GATTGAAATT AAGCAGCGTC TGATTGAGGA GTTAGAGCAA 
AGTCAGCGGA GACTACAAAC ACTCAAACTT CAGTATGAGG AAAAATTGCA GCAACTCCAG 
AGTCGGATTC GTGATACACA GCTGGAAAGG GACCGAGTGC TACGGGAACT TAGCTCCATG 
GAGTGTTACA CAGAGGAAAA GGCTAACAAG ATCCGTGCTG ATTATGAGAA AAAGCTTCGG 
GAAATGAATC GAGACTTGCA GAAGCTGCAG TTGGCTCAGA GAGAGCATAC ACGACTCCTT 
AAAAATCAGT CCCGTTATGA ACGTGAACTT AAGAAATTGC AGATGGAACT AAGTGATATG 
AAGAAGGCCA AATCAGCTCT AATGAAGCAG ATGCGGGAAG AACAGCAGCT CAGAAAACTT 
GCAGAATCTA GAAGGAACAA AGAAATAGCT CAGCTGAAGA AGGAGCACCA TAGACAAGAG 
CTTCACATTA GAGCTTTGGA GTCCCAGAAG AGACAGCAAG AGATAGTGCT GAGACGCAAG 
ACCCAAGAGG TTTCTGCTCT GCGGCGTCTC TCTAGGCGAC AAACTGATCG ATCTGGGGGA 
CGAAGCCACT CTGTTGCAGA CTCTGGAGCA GAGTTGTCCA CAAGCACAGC ATCTTCAGAA 
CCAGATAGTT CTTTACGTTC TGTGTCCAGT ATTGTCCGTC ATTGGAACCG AAAGACAAGT 
GGAGACGTTG GGCCAGTGCC CAATGGGAGT CGTACACCAA GAAGGAAAGG GGTACGTCCA 
GGCCTACCAC TCAGTAAGGC TGCTCACATG AAGTGGCAAA CTCTGGAGAG AAGGGTTCAT 
GAAGTCGTCC TACAGAGACT TACGCTTCTT AACTTAGAAA GCGATATGGA AAGACTGTTG 
CGGAAACGTG AGGAGCTGTC ACTTCTGCAG GAAGCTTTGA TAAGTCGTTC CATAGCTCTC 
CCAGAAGACA GGAAAGCAGA GGAAAAGTCA CAGCAGGATG TTGCCGAAGA ACTGGAAGCT 
TTGACAGCCA ATATTGATTA TGTTAATGAC TGCATTCGAG AATGCCAGGC TACCATTGTT 
CAGATGGAGG AAACAAAGGA GGATCTAGAC TCAACAGACA CCTCAGTTGT GATCAGTTCT 
TGTTCTCTAA CTGAGGCACG ACATCTTCTT GATAATTTCC TGAGAGCCTC AATAGATAAG 
GGCCTGCAAG TAGCTCAGAA AGAAGCCCAG ATACGGATTC TGGAAGCCCG GATTCGGCAA 
ACTGACATGG CTGGCCTGTC ACAGGCACAC ATGCTGCTGG AGAAATCAGA GTGCCATTCT 
GTGCAGCAGG CAGAAAACGG GTATGCTAGC ACAGATGAAG AGGTGTCAGA AATTAGCCAG 
ACTTCTGATG GAAGTGCTTC TTTCTCCTCC ATGAAAGGAT CCTGCAGTCA AGATGACTTT 
AAGTGGAAGG GGGAACCACG GCTTTCATGG CAAGTGAAGG CCGTGTCTGC TGAGTCTTTG 
AGTCCGCTGC TTGAAGCTTC CACTAGAAAC ATCACCAAAT CCTTAGCATC ATTAACTGAC 
ATCCTAGAAG ATGTTGGGGG ATTCTCTCCG AGTGGTTTGC GGGGCAGAGC ATCACACAGC 
CTTACTCTTC CAATACGTGG TAATACATTT CCGAGACAAT CCAGAGGTTT CAACACATCC 
CCTGTGACAC GCAGAAAATC ATATGACAGA GGACAGCCAA GGGCTTCAGA CTCAAACCTA 
ACACCTCCAT CATCTCCCCC TATGCGCCCT CGTAATGATA AAAATGTCTT TTCTCGGCTA 
ACAAGCAGTC AGAGTTCAGG CACTGCACTG GATAAGTCTG ATGAGAGTGA CAGCTCTTTC 
TCAGATGTCT CCAGGGGCAT AATAAGTCAA TTTGGAGGGC TAAAGAGCAT GAGGACAGCT 
CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA CATACAAAGC CAGTTCTTTG TGTTGAAGCT 
ACAGATGAGC TCCTCTTTAC AGGATCTAAA GATCGCACAT GCAAACTCTG GAATCTGGTA 
ACAGGGCAGG AAATTGCATC CCTTCGCAAC CATCCCCATA ACGTTGTATC TCTGAAACAT 
TGCCCAAACT CTGGGCTAGT TCTCACAGCA TCTGCTTCTT GTGTGCGAGT ATGGGATATC 
CGTGACAGTG CTCGCTGTGT GAGGACATTG TTGTCATCAG GCCAGGTAAT GTCAGGAGAT 
ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA AGCAGCACTC AGGCAGAGTT GCAAATTAAT 
CAAATAACTT TAAATCCTGA TGGCACAATC CTCTATGCAG CAGCCGGCAA TGCAGTGAGA 
GCTTGGGACC TAAGCAGGTT TCAACCCATT GGAAAGCTAA CAGGACACAT GGCTCCAGTC 
ATGTGTCTTA CAGTGAGCAA CACACAAAAC GGACGAGATC TTGTTATCAC AGGATCTAAA 
GATCACTATG TCAAGATATT TGAACTCCAT TCTGGATGGG GAGGGGCAGT TCCCCCCACT 
TATAACCTGG AACCTCCTCA TTACGATGGT ATTGAGTGCT TGGCTGTACA GAATGGGATC 
CTTTGCAGTG GCTCTCGAGA TAATGGAATC AAAAAGTGGG ATCTACAGCA ACATGAGCTC 
ACTCAGCAAG TGCCAAATGC ACACAGAGAC TGGATTTGTG CTTTAGGATT TGTACCGGGA 
CGTTCAATGT TGCTTAGTGG ATGTCGTGGT GGCATTCTAA AGGTTTGGAA CATGGAGAAC 
TTTACACCTA TTGGGGAGAT CAAAGGCCAC GAAAGCCCCA TTAATGCGAT GTGTTCTAAT 
AGCCGTCACA TCTTCACTGC ATCTAGTGAC TGCCGAGTCA AGCTATGGGT TTATGTTCCT 
GGTCTTACTC CATGTCTCCC TCGACGTGTT CTGGCAATCA AGGGCCGTGC CACTAGCCTT 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826406.1
CDS	190..4935
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGEKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970952.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970952.2

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGAAG 
GACAGTTCTT CTTTGGAGTA TGAGACTTTG ACTGCTAAAT TTCACTTTGT GGACCTGGCA 
GGATCTGAGC GCCTCAAGCG TACAGGAGCA ACTGGAGAGA GAGCAAGAGA AGGGATCTCT 
ATAAATTGTG GCCTTCTGGC ACTTGGGAAT GTAATCAGTG CTCTTGGAGA TCAAAGTAAG 
AAGGTTCTTC ATGTTCCATA CAGAGATTCA AAACTGACTC GACTACTGCA GGATTCATTA 
GGTGGTAACA GTCAAACCAT GATGATTGCT TGCGTTTCTC CATCTGATCG TGATTTCATG 
GAAACCTTAA ACACTCTAAA GTATGCAAAT CGTGCACGAA ACATTAAGAA TCGTGTAACA 
GCCAATCAGG AGCAGAGTGG ACAGCAAGTA CAGGCTCTTC GGGCAGAACT AGCCAGAGTA 
AAGTTGGAAT TACAAGAGTA CAAGACAGGT CATCGAGTTG CTGGTAATGA TAGCTTAAGC 
GATATGTGTC AGGAGCTGTC TATGTTACGT AGTGAGAATG CATCATTGCG GCTTCGTGTT 
ACTGCCCTAC AAGAGGCTAT TAGAGCTATC AATGCTCGTG TCACTCGTAT GGCTGGACAA 
GAAGCTGCAC ACTTGGTGCA GCGAGCTGGT GATAGTAATG AAGAAATACA AACTCTCATA 
CAGAACTATA TCCATGAAAT AGAAGAACTC AGAACCCGTC TGGTAGAAAG TGAAGCAATG 
AATGAATCTC TCAGACGCAG TTTGTCCCGT TCAACTGCAC GCTCATTGGT AACAGGACCT 
GCAACCCCTA CCCCATCATT AGAAAAGGAG GCCAATGAAG TTGTTCGAAG AGCAAAAGAA 
GAAGTACAGA ATTTGAAAAA GAAAGGAAGT TCATTACAGT CTGATAACTA CAAAAAGAGC 
TTGAAACAAC AACTGCAAAT CAGTGAAGAG ACAGATTATA ATGAAGGAGA AGAGGAGGAT 
GACCATGAAG AAAGTGAATG CGATGAAGAG GAAAGTGAGG GAAGTGGGGA GAGTGAGGCA 
GAGTCAGACT CAGAACCCGA AGAGAAAGGG TTGGGCTGTT GGGACGCAGG GAAAAAATCC 
AGTGGGCCCC TGTCCTTGTG GGCCCCTCTG ACCCAAGCCG GATCCCTGCA AGCGCCCCCA 
CCCAGTCAGC CCTCACCCAG TTTGCCATTC TGCATGGAGG AAGGCTCACT TGGAGGAGAC 
CTTGCTGACT TGACTTGTGA GATTGAAATT AAGCAGCGTC TGATTGAGGA GTTAGAGCAA 
AGTCAGCGGA GACTACAAAC ACTCAAACTT CAGTATGAGG AAAAATTGCA GCAACTCCAG 
AGTCGGATTC GTGATACACA GCTGGAAAGG GACCGAGTGC TACGGGAACT TAGCTCCATG 
GAGTGTTACA CAGAGGAAAA GGCTAACAAG ATCCGTGCTG ATTATGAGAA AAAGCTTCGG 
GAAATGAATC GAGACTTGCA GAAGCTGCAG TTGGCTCAGA GAGAGCATAC ACGACTCCTT 
AAAAATCAGT CCCGTTATGA ACGTGAACTT AAGAAATTGC AGATGGAACT AAGTGATATG 
AAGAAGGCCA AATCAGCTCT AATGAAGCAG ATGCGGGAAG AACAGCAGCT CAGAAAACTT 
GCAGAATCTA GAAGGAACAA AGAAATAGCT CAGCTGAAGA AGGAGCACCA TAGACAAGAG 
CTTCACATTA GAGCTTTGGA GTCCCAGAAG AGACAGCAAG AGATAGTGCT GAGACGCAAG 
ACCCAAGAGG TTTCTGCTCT GCGGCGTCTC TCTAGGCGAC AAACTGATCG ATCTGGGGGA 
CGAAGCCACT CTGTTGCAGA CTCTGGAGCA GAGTTGTCCA CAAGCACAGC ATCTTCAGAA 
CCAGATAGTT CTTTACGTTC TGTGTCCAGT ATTGTCCGTC ATTGGAACCG AAAGACAAGT 
GGAGACGTTG GGCCAGTGCC CAATGGGAGT CGTACACCAA GAAGGAAAGG GGTACGTCCA 
GGCCTACCAC TCAGTAAGGC TGCTCACATG AAGTGGCAAA CTCTGGAGAG AAGGGTTCAT 
GAAGTCGTCC TACAGAGACT TACGCTTCTT AACTTAGAAA GCGATATGGA AAGACTGTTG 
CGGAAACGTG AGGAGCTGTC ACTTCTGCAG GAAGCTTTGA TAAGTCGTTC CATAGCTCTC 
CCAGAAGACA GGAAAGCAGA GGAAAAGTCA CAGCAGGATG TTGCCGAAGA ACTGGAAGCT 
TTGACAGCCA ATATTGATTA TGTTAATGAC TGCATTCGAG AATGCCAGGC TACCATTGTT 
CAGATGGAGG AAACAAAGGA GGATCTAGAC TCAACAGACA CCTCAGTTGT GATCAGTTCT 
TGTTCTCTAA CTGAGGCACG ACATCTTCTT GATAATTTCC TGAGAGCCTC AATAGATAAG 
GGCCTGCAAG TAGCTCAGAA AGAAGCCCAG ATACGGATTC TGGAAGCCCG GATTCGGCAA 
ACTGACATGG CTGGCCTGTC ACAGGCACAC ATGCTGCTGG AGAAATCAGA GTGCCATTCT 
GTGCAGCAGG CAGAAAACGG GTATGCTAGC ACAGATGAAG AGGTGTCAGA AATTAGCCAG 
ACTTCTGATG GAAGTGCTTC TTTCTCCTCC ATGAAAGGAT CCTGCAGTCA AGATGACTTT 
AAGTGGAAGG GGGAACCACG GCTTTCATGG CAAGTGAAGG CCGTGTCTGC TGAGTCTTTG 
AGTCCGCTGC TTGAAGCTTC CACTAGAAAC ATCACCAAAT CCTTAGCATC ATTAACTGAC 
ATCCTAGAAG ATGTTGGGGG ATTCTCTCCG AGTGGTTTGC GGGGCAGAGC ATCACACAGC 
CTTACTCTTC CAATACGTGG TAATACATTT CCGAGACAAT CCAGAGGTTT CAACACATCC 
CCTGTGACAC GCAGAAAATC ATATGACAGA GGACAGCCAA GGGCTTCAGA CTCAAACCTA 
ACACCTCCAT CATCTCCCCC TATGCGCCCT CGTAATGATA AAAATGTCTT TTCTCGGCTA 
ACAAGCAGTC AGAGTTCAGG CACTGCACTG GATAAGTCTG ATGAGAGTGA CAGCTCTTTC 
TCAGATGTCT CCAGGGGCAT AATAAGTCAA TTTGGAGGGC TAAAGAGCAT GAGGACAGCT 
CCTTTGCAGT GTGTTGCAAT TGCTGAGGGA CATACAAAGC CAGTTCTTTG TGTTGAAGCT 
ACAGATGAGC TCCTCTTTAC AGGATCTAAA GATCGCACAT GCAAACTCTG GAATCTGGTA 
ACAGGGCAGG AAATTGCATC CCTTCGCAAC CATCCCCATA ACGTTGTATC TCTGAAACAT 
TGCCCAAACT CTGGGCTAGT TCTCACAGCA TCTGCTTCTT GTGTGCGAGT ATGGGATATC 
CGTGACAGTG CTCGCTGTGT GAGGACATTG TTGTCATCAG GCCAGGTAAT GTCAGGAGAT 
ATCTGCTCTG GGCTAGGTTT CCGCTCAGGA AGCAGCACTC AGGCAGAGTT GCAAATTAAT 
CAAATAACTT TAAATCCTGA TGGCACAATC CTCTATGCAG CAGCCGGCAA TGCAGTGAGA 
GCTTGGGACC TAAGCAGGTT TCAACCCATT GGAAAGCTAA CAGGACACAT GGCTCCAGTC 
ATGTGTCTTA CAGTGAGCAA CACACAAAAC GGACGAGATC TTGTTATCAC AGGATCTAAA 
GATCACTATG TCAAGATATT TGAACTCCAT TCTGGATGGG GAGGGGCAGT TCCCCCCACT 
TATAACCTGG AACCTCCTCA TTACGATGGT ATTGAGTGCT TGGCTGTACA GAATGGGATC 
CTTTGCAGTG GCTCTCGAGA TAATGGAATC AAAAAGTGGG ATCTACAGCA ACATGAGCTC 
ACTCAGCAAG TGCCAAATGC ACACAGAGAC TGGATTTGTG CTTTAGGATT TGTACCGGGA 
CGTTCAATGT TGCTTAGTGG ATGTCGTGGT GGCATTCTAA AGGTTTGGAA CATGGAGAAC 
TTTACACCTA TTGGGGAGAT CAAAGGCCAC GAAAGCCCCA TTAATGCGAT GTGTTCTAAT 
AGCCGTCACA TCTTCACTGC ATCTAGTGAC TGCCGAGTCA AGCTATGGGT TTATGTTCCT 
GGTCTTACTC CATGTCTCCC TCGACGTGTT CTGGCAATCA AGGGCCGTGC CACTAGCCTT 
CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970955.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826409.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X11, mRNA.

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CloneID	OXa29030
Clone ID Related Accession (Same CDS sequence)	XM_012970955.3 , XM_012970955.2
Accession Version	XM_012970955.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT 
GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT 
CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT 
CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG 
TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA 
ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA 
AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG 
AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA 
GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT 
CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC 
CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA 
AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA 
GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA 
GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC 
CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA 
GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG 
AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA 
GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG 
ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG 
ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT 
TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC 
CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT 
GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG 
CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT 
TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG 
TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT 
CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC 
CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT 
ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT 
GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA 
CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA 
AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA 
GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT 
TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA 
GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA 
GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC 
CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT 
GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC 
TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA 
ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT 
TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG 
TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT 
CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT 
AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT 
TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT 
CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT 
TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT 
ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC 
CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT 
CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826409.1
CDS	189..4931
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970955.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970955.2

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GCTCACTTGG AGGAGACCTT 
GCTGACTTGA CTTGTGAGAT TGAAATTAAG CAGCGTCTGA TTGAGGAGTT AGAGCAAAGT 
CAGCGGAGAC TACAAACACT CAAACTTCAG TATGAGGAAA AATTGCAGCA ACTCCAGAGT 
CGGATTCGTG ATACACAGCT GGAAAGGGAC CGAGTGCTAC GGGAACTTAG CTCCATGGAG 
TGTTACACAG AGGAAAAGGC TAACAAGATC CGTGCTGATT ATGAGAAAAA GCTTCGGGAA 
ATGAATCGAG ACTTGCAGAA GCTGCAGTTG GCTCAGAGAG AGCATACACG ACTCCTTAAA 
AATCAGTCCC GTTATGAACG TGAACTTAAG AAATTGCAGA TGGAACTAAG TGATATGAAG 
AAGGCCAAAT CAGCTCTAAT GAAGCAGATG CGGGAAGAAC AGCAGCTCAG AAAACTTGCA 
GAATCTAGAA GGAACAAAGA AATAGCTCAG CTGAAGAAGG AGCACCATAG ACAAGAGCTT 
CACATTAGAG CTTTGGAGTC CCAGAAGAGA CAGCAAGAGA TAGTGCTGAG ACGCAAGACC 
CAAGAGGTTT CTGCTCTGCG GCGTCTCTCT AGGCGACAAA CTGATCGATC TGGGGGACGA 
AGCCACTCTG TTGCAGACTC TGGAGCAGAG TTGTCCACAA GCACAGCATC TTCAGAACCA 
GATAGTTCTT TACGTTCTGT GTCCAGTATT GTCCGTCATT GGAACCGAAA GACAAGTGGA 
GACGTTGGGC CAGTGCCCAA TGGGAGTCGT ACACCAAGAA GGAAAGGGGT ACGTCCAGGC 
CTACCACTCA GTAAGGCTGC TCACATGAAG TGGCAAACTC TGGAGAGAAG GGTTCATGAA 
GTCGTCCTAC AGAGACTTAC GCTTCTTAAC TTAGAAAGCG ATATGGAAAG ACTGTTGCGG 
AAACGTGAGG AGCTGTCACT TCTGCAGGAA GCTTTGATAA GTCGTTCCAT AGCTCTCCCA 
GAAGACAGGA AAGCAGAGGA AAAGTCACAG CAGGATGTTG CCGAAGAACT GGAAGCTTTG 
ACAGCCAATA TTGATTATGT TAATGACTGC ATTCGAGAAT GCCAGGCTAC CATTGTTCAG 
ATGGAGGAAA CAAAGGAGGA TCTAGACTCA ACAGACACCT CAGTTGTGAT CAGTTCTTGT 
TCTCTAACTG AGGCACGACA TCTTCTTGAT AATTTCCTGA GAGCCTCAAT AGATAAGGGC 
CTGCAAGTAG CTCAGAAAGA AGCCCAGATA CGGATTCTGG AAGCCCGGAT TCGGCAAACT 
GACATGGCTG GCCTGTCACA GGCACACATG CTGCTGGAGA AATCAGAGTG CCATTCTGTG 
CAGCAGGCAG AAAACGGGTA TGCTAGCACA GATGAAGAGG TGTCAGAAAT TAGCCAGACT 
TCTGATGGAA GTGCTTCTTT CTCCTCCATG AAAGGATCCT GCAGTCAAGA TGACTTTAAG 
TGGAAGGGGG AACCACGGCT TTCATGGCAA GTGAAGGCCG TGTCTGCTGA GTCTTTGAGT 
CCGCTGCTTG AAGCTTCCAC TAGAAACATC ACCAAATCCT TAGCATCATT AACTGACATC 
CTAGAAGATG TTGGGGGATT CTCTCCGAGT GGTTTGCGGG GCAGAGCATC ACACAGCCTT 
ACTCTTCCAA TACGTGGTAA TACATTTCCG AGACAATCCA GAGGTTTCAA CACATCCCCT 
GTGACACGCA GAAAATCATA TGACAGAGGA CAGCCAAGGG CTTCAGACTC AAACCTAACA 
CCTCCATCAT CTCCCCCTAT GCGCCCTCGT AATGATAAAA ATGTCTTTTC TCGGCTAACA 
AGCAGTCAGA GTTCAGGCAC TGCACTGGAT AAGTCTGATG AGAGTGACAG CTCTTTCTCA 
GATGTCTCCA GGGGCATAAT AAGTCAATTT GGAGGGCTAA AGAGCATGAG GACAGCTCCT 
TTGCAGTGTG TTGCAATTGC TGAGGGACAT ACAAAGCCAG TTCTTTGTGT TGAAGCTACA 
GATGAGCTCC TCTTTACAGG ATCTAAAGAT CGCACATGCA AACTCTGGAA TCTGGTAACA 
GGGCAGGAAA TTGCATCCCT TCGCAACCAT CCCCATAACG TTGTATCTCT GAAACATTGC 
CCAAACTCTG GGCTAGTTCT CACAGCATCT GCTTCTTGTG TGCGAGTATG GGATATCCGT 
GACAGTGCTC GCTGTGTGAG GACATTGTTG TCATCAGGCC AGGTAATGTC AGGAGATATC 
TGCTCTGGGC TAGGTTTCCG CTCAGGAAGC AGCACTCAGG CAGAGTTGCA AATTAATCAA 
ATAACTTTAA ATCCTGATGG CACAATCCTC TATGCAGCAG CCGGCAATGC AGTGAGAGCT 
TGGGACCTAA GCAGGTTTCA ACCCATTGGA AAGCTAACAG GACACATGGC TCCAGTCATG 
TGTCTTACAG TGAGCAACAC ACAAAACGGA CGAGATCTTG TTATCACAGG ATCTAAAGAT 
CACTATGTCA AGATATTTGA ACTCCATTCT GGATGGGGAG GGGCAGTTCC CCCCACTTAT 
AACCTGGAAC CTCCTCATTA CGATGGTATT GAGTGCTTGG CTGTACAGAA TGGGATCCTT 
TGCAGTGGCT CTCGAGATAA TGGAATCAAA AAGTGGGATC TACAGCAACA TGAGCTCACT 
CAGCAAGTGC CAAATGCACA CAGAGACTGG ATTTGTGCTT TAGGATTTGT ACCGGGACGT 
TCAATGTTGC TTAGTGGATG TCGTGGTGGC ATTCTAAAGG TTTGGAACAT GGAGAACTTT 
ACACCTATTG GGGAGATCAA AGGCCACGAA AGCCCCATTA ATGCGATGTG TTCTAATAGC 
CGTCACATCT TCACTGCATC TAGTGACTGC CGAGTCAAGC TATGGGTTTA TGTTCCTGGT 
CTTACTCCAT GTCTCCCTCG ACGTGTTCTG GCAATCAAGG GCCGTGCCAC TAGCCTTCCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970961.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826415.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa29031
Clone ID Related Accession (Same CDS sequence)	XM_012970961.2
Accession Version	XM_012970961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGGCATA 
ATAAGTCAAT TTGGAGGGCT AAAGAGCATG AGGACAGCTC CTTTGCAGTG TGTTGCAATT 
GCTGAGGGAC ATACAAAGCC AGTTCTTTGT GTTGAAGCTA CAGATGAGCT CCTCTTTACA 
GGATCTAAAG ATCGCACATG CAAACTCTGG AATCTGGTAA CAGGGCAGGA AATTGCATCC 
CTTCGCAACC ATCCCCATAA CGTTGTATCT CTGAAACATT GCCCAAACTC TGGGCTAGTT 
CTCACAGCAT CTGCTTCTTG TGTGCGAGTA TGGGATATCC GTGACAGTGC TCGCTGTGTG 
AGGACATTGT TGTCATCAGG CCAGGTAATG TCAGGAGATA TCTGCTCTGG GCTAGGTTTC 
CGCTCAGGAA GCAGCACTCA GGCAGAGTTG CAAATTAATC AAATAACTTT AAATCCTGAT 
GGCACAATCC TCTATGCAGC AGCCGGCAAT GCAGTGAGAG CTTGGGACCT AAGCAGGTTT 
CAACCCATTG GAAAGCTAAC AGGACACATG GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC 
ACACAAAACG GACGAGATCT TGTTATCACA GGATCTAAAG ATCACTATGT CAAGATATTT 
GAACTCCATT CTGGATGGGG AGGGGCAGTT CCCCCCACTT ATAACCTGGA ACCTCCTCAT 
TACGATGGTA TTGAGTGCTT GGCTGTACAG AATGGGATCC TTTGCAGTGG CTCTCGAGAT 
AATGGAATCA AAAAGTGGGA TCTACAGCAA CATGAGCTCA CTCAGCAAGT GCCAAATGCA 
CACAGAGACT GGATTTGTGC TTTAGGATTT GTACCGGGAC GTTCAATGTT GCTTAGTGGA 
TGTCGTGGTG GCATTCTAAA GGTTTGGAAC ATGGAGAACT TTACACCTAT TGGGGAGATC 
AAAGGCCACG AAAGCCCCAT TAATGCGATG TGTTCTAATA GCCGTCACAT CTTCACTGCA 
TCTAGTGACT GCCGAGTCAA GCTATGGGTT TATGTTCCTG GTCTTACTCC ATGTCTCCCT 
CGACGTGTTC TGGCAATCAA GGGCCGTGCC ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826415.1
CDS	190..4914
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLSSGQVMSGDICSGLGFRSGSSTQAELQINQITLNPDGTILYAAAGNAVRAWDLSRFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970961.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970961.2

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGGCATA 
ATAAGTCAAT TTGGAGGGCT AAAGAGCATG AGGACAGCTC CTTTGCAGTG TGTTGCAATT 
GCTGAGGGAC ATACAAAGCC AGTTCTTTGT GTTGAAGCTA CAGATGAGCT CCTCTTTACA 
GGATCTAAAG ATCGCACATG CAAACTCTGG AATCTGGTAA CAGGGCAGGA AATTGCATCC 
CTTCGCAACC ATCCCCATAA CGTTGTATCT CTGAAACATT GCCCAAACTC TGGGCTAGTT 
CTCACAGCAT CTGCTTCTTG TGTGCGAGTA TGGGATATCC GTGACAGTGC TCGCTGTGTG 
AGGACATTGT TGTCATCAGG CCAGGTAATG TCAGGAGATA TCTGCTCTGG GCTAGGTTTC 
CGCTCAGGAA GCAGCACTCA GGCAGAGTTG CAAATTAATC AAATAACTTT AAATCCTGAT 
GGCACAATCC TCTATGCAGC AGCCGGCAAT GCAGTGAGAG CTTGGGACCT AAGCAGGTTT 
CAACCCATTG GAAAGCTAAC AGGACACATG GCTCCAGTCA TGTGTCTTAC AGTGAGCAAC 
ACACAAAACG GACGAGATCT TGTTATCACA GGATCTAAAG ATCACTATGT CAAGATATTT 
GAACTCCATT CTGGATGGGG AGGGGCAGTT CCCCCCACTT ATAACCTGGA ACCTCCTCAT 
TACGATGGTA TTGAGTGCTT GGCTGTACAG AATGGGATCC TTTGCAGTGG CTCTCGAGAT 
AATGGAATCA AAAAGTGGGA TCTACAGCAA CATGAGCTCA CTCAGCAAGT GCCAAATGCA 
CACAGAGACT GGATTTGTGC TTTAGGATTT GTACCGGGAC GTTCAATGTT GCTTAGTGGA 
TGTCGTGGTG GCATTCTAAA GGTTTGGAAC ATGGAGAACT TTACACCTAT TGGGGAGATC 
AAAGGCCACG AAAGCCCCAT TAATGCGATG TGTTCTAATA GCCGTCACAT CTTCACTGCA 
TCTAGTGACT GCCGAGTCAA GCTATGGGTT TATGTTCCTG GTCTTACTCC ATGTCTCCCT 
CGACGTGTTC TGGCAATCAA GGGCCGTGCC ACTAGCCTTC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif21b ( XM_012970966.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826420.1 Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OXa29032
Clone ID Related Accession (Same CDS sequence)	XM_012970966.2
Accession Version	XM_012970966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF21B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012970966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTTTCA ACCCATTGGA 
AAGCTAACAG GACACATGGC TCCAGTCATG TGTCTTACAG TGAGCAACAC ACAAAACGGA 
CGAGATCTTG TTATCACAGG ATCTAAAGAT CACTATGTCA AGATATTTGA ACTCCATTCT 
GGATGGGGAG GGGCAGTTCC CCCCACTTAT AACCTGGAAC CTCCTCATTA CGATGGTATT 
GAGTGCTTGG CTGTACAGAA TGGGATCCTT TGCAGTGGCT CTCGAGATAA TGGAATCAAA 
AAGTGGGATC TACAGCAACA TGAGCTCACT CAGCAAGTGC CAAATGCACA CAGAGACTGG 
ATTTGTGCTT TAGGATTTGT ACCGGGACGT TCAATGTTGC TTAGTGGATG TCGTGGTGGC 
ATTCTAAAGG TTTGGAACAT GGAGAACTTT ACACCTATTG GGGAGATCAA AGGCCACGAA 
AGCCCCATTA ATGCGATGTG TTCTAATAGC CGTCACATCT TCACTGCATC TAGTGACTGC 
CGAGTCAAGC TATGGGTTTA TGTTCCTGGT CTTACTCCAT GTCTCCCTCG ACGTGTTCTG 
GCAATCAAGG GCCGTGCCAC TAGCCTTCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826420.1
CDS	190..4902
Translation	MGSQGDCSVRVALRIRPQSSKEQTEGCRICTWVYAGEPQVLLGKDKAFTYDFVFDMDTDQIQVYETCVRGLVEGCFQGYNATVLAYGQTGAGKTHTMGSGFDLVVTEEELGIIPRAVRQLFSTIQEHKASAYERGLPEPSFKVSAQFLELYNEEILDLFDVSRDPDSRYRKSNIRIHEDASGGIYVSGASIRSVSSEGELLQLLKEGALSRTTASTQMNSQSSRSHAIFSVHLQQTRVSETCSKDSSSLEYETLTAKFHFVDLAGSERLKRTGATGERAREGISINCGLLALGNVISALGDQSKKVLHVPYRDSKLTRLLQDSLGGNSQTMMIACVSPSDRDFMETLNTLKYANRARNIKNRVTANQEQSGQQVQALRAELARVKLELQEYKTGHRVAGNDSLSDMCQELSMLRSENASLRLRVTALQEAIRAINARVTRMAGQEAAHLVQRAGDSNEEIQTLIQNYIHEIEELRTRLVESEAMNESLRRSLSRSTARSLVTGPATPTPSLEKEANEVVRRAKEEVQNLKKKGSSLQSDNYKKSLKQQLQISEETDYNEGEEEDDHEESECDEEESEGSGESEAESDSEPEEKGLGCWDAGKKSSGPLSLWAPLTQAGSLQAPPPSQPSPSLPFCMEEGSLGGDLADLTCEIEIKQRLIEELEQSQRRLQTLKLQYEEKLQQLQSRIRDTQLERDRVLRELSSMECYTEEKANKIRADYEKKLREMNRDLQKLQLAQREHTRLLKNQSRYERELKKLQMELSDMKKAKSALMKQMREEQQLRKLAESRRNKEIAQLKKEHHRQELHIRALESQKRQQEIVLRRKTQEVSALRRLSRRQTDRSGGRSHSVADSGAELSTSTASSEPDSSLRSVSSIVRHWNRKTSGDVGPVPNGSRTPRRKGVRPGLPLSKAAHMKWQTLERRVHEVVLQRLTLLNLESDMERLLRKREELSLLQEALISRSIALPEDRKAEEKSQQDVAEELEALTANIDYVNDCIRECQATIVQMEETKEDLDSTDTSVVISSCSLTEARHLLDNFLRASIDKGLQVAQKEAQIRILEARIRQTDMAGLSQAHMLLEKSECHSVQQAENGYASTDEEVSEISQTSDGSASFSSMKGSCSQDDFKWKGEPRLSWQVKAVSAESLSPLLEASTRNITKSLASLTDILEDVGGFSPSGLRGRASHSLTLPIRGNTFPRQSRGFNTSPVTRRKSYDRGQPRASDSNLTPPSSPPMRPRNDKNVFSRLTSSQSSGTALDKSDESDSSFSDVSRGIISQFGGLKSMRTAPLQCVAIAEGHTKPVLCVEATDELLFTGSKDRTCKLWNLVTGQEIASLRNHPHNVVSLKHCPNSGLVLTASASCVRVWDIRDSARCVRTLLFQPIGKLTGHMAPVMCLTVSNTQNGRDLVITGSKDHYVKIFELHSGWGGAVPPTYNLEPPHYDGIECLAVQNGILCSGSRDNGIKKWDLQQHELTQQVPNAHRDWICALGFVPGRSMLLSGCRGGILKVWNMENFTPIGEIKGHESPINAMCSNSRHIFTASSDCRVKLWVYVPGLTPCLPRRVLAIKGRATSLP

Target ORF information:

RefSeq Version	XM_012970966.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 21B (kif21b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012970966.2

ORF Insert Sequence:

ATGGGGAGCC AGGGGGACTG CAGTGTCAGG GTTGCGCTGA GGATTCGACC ACAGAGCAGT 
AAGGAGCAGA CTGAAGGATG CCGTATATGT ACATGGGTTT ATGCTGGAGA GCCACAGGTG 
CTTTTGGGTA AAGACAAAGC CTTCACATAT GATTTTGTGT TTGATATGGA CACAGACCAA 
ATCCAAGTCT ATGAGACTTG TGTACGTGGG CTGGTAGAAG GTTGTTTTCA AGGCTACAAT 
GCTACTGTGC TGGCCTATGG ACAGACTGGT GCTGGTAAAA CACATACAAT GGGTAGTGGA 
TTTGATCTTG TGGTAACTGA GGAAGAACTG GGGATCATCC CACGTGCTGT GCGTCAGCTT 
TTCAGCACCA TACAAGAGCA CAAAGCTTCT GCTTATGAGA GAGGACTGCC AGAGCCCAGC 
TTTAAAGTTA GTGCACAATT CTTAGAACTC TATAATGAGG AAATCTTGGA TCTATTTGAT 
GTATCCCGTG ATCCTGACTC TCGCTATCGG AAATCTAATA TTCGGATCCA CGAGGATGCT 
AGCGGAGGGA TCTATGTTAG TGGTGCCAGC ATTCGCAGTG TCAGCTCAGA AGGAGAGCTA 
CTACAGCTTC TAAAGGAGGG TGCTCTTTCT AGAACTACAG CCAGTACCCA GATGAATTCC 
CAGAGTTCAC GGTCACATGC TATCTTCTCA GTCCATCTCC AACAAACACG TGTTTCTGAG 
ACTTGCTCGA AGGACAGTTC TTCTTTGGAG TATGAGACTT TGACTGCTAA ATTTCACTTT 
GTGGACCTGG CAGGATCTGA GCGCCTCAAG CGTACAGGAG CAACTGGAGA GAGAGCAAGA 
GAAGGGATCT CTATAAATTG TGGCCTTCTG GCACTTGGGA ATGTAATCAG TGCTCTTGGA 
GATCAAAGTA AGAAGGTTCT TCATGTTCCA TACAGAGATT CAAAACTGAC TCGACTACTG 
CAGGATTCAT TAGGTGGTAA CAGTCAAACC ATGATGATTG CTTGCGTTTC TCCATCTGAT 
CGTGATTTCA TGGAAACCTT AAACACTCTA AAGTATGCAA ATCGTGCACG AAACATTAAG 
AATCGTGTAA CAGCCAATCA GGAGCAGAGT GGACAGCAAG TACAGGCTCT TCGGGCAGAA 
CTAGCCAGAG TAAAGTTGGA ATTACAAGAG TACAAGACAG GTCATCGAGT TGCTGGTAAT 
GATAGCTTAA GCGATATGTG TCAGGAGCTG TCTATGTTAC GTAGTGAGAA TGCATCATTG 
CGGCTTCGTG TTACTGCCCT ACAAGAGGCT ATTAGAGCTA TCAATGCTCG TGTCACTCGT 
ATGGCTGGAC AAGAAGCTGC ACACTTGGTG CAGCGAGCTG GTGATAGTAA TGAAGAAATA 
CAAACTCTCA TACAGAACTA TATCCATGAA ATAGAAGAAC TCAGAACCCG TCTGGTAGAA 
AGTGAAGCAA TGAATGAATC TCTCAGACGC AGTTTGTCCC GTTCAACTGC ACGCTCATTG 
GTAACAGGAC CTGCAACCCC TACCCCATCA TTAGAAAAGG AGGCCAATGA AGTTGTTCGA 
AGAGCAAAAG AAGAAGTACA GAATTTGAAA AAGAAAGGAA GTTCATTACA GTCTGATAAC 
TACAAAAAGA GCTTGAAACA ACAACTGCAA ATCAGTGAAG AGACAGATTA TAATGAAGGA 
GAAGAGGAGG ATGACCATGA AGAAAGTGAA TGCGATGAAG AGGAAAGTGA GGGAAGTGGG 
GAGAGTGAGG CAGAGTCAGA CTCAGAACCC GAAGAGAAAG GGTTGGGCTG TTGGGACGCA 
GGGAAAAAAT CCAGTGGGCC CCTGTCCTTG TGGGCCCCTC TGACCCAAGC CGGATCCCTG 
CAAGCGCCCC CACCCAGTCA GCCCTCACCC AGTTTGCCAT TCTGCATGGA GGAAGGCTCA 
CTTGGAGGAG ACCTTGCTGA CTTGACTTGT GAGATTGAAA TTAAGCAGCG TCTGATTGAG 
GAGTTAGAGC AAAGTCAGCG GAGACTACAA ACACTCAAAC TTCAGTATGA GGAAAAATTG 
CAGCAACTCC AGAGTCGGAT TCGTGATACA CAGCTGGAAA GGGACCGAGT GCTACGGGAA 
CTTAGCTCCA TGGAGTGTTA CACAGAGGAA AAGGCTAACA AGATCCGTGC TGATTATGAG 
AAAAAGCTTC GGGAAATGAA TCGAGACTTG CAGAAGCTGC AGTTGGCTCA GAGAGAGCAT 
ACACGACTCC TTAAAAATCA GTCCCGTTAT GAACGTGAAC TTAAGAAATT GCAGATGGAA 
CTAAGTGATA TGAAGAAGGC CAAATCAGCT CTAATGAAGC AGATGCGGGA AGAACAGCAG 
CTCAGAAAAC TTGCAGAATC TAGAAGGAAC AAAGAAATAG CTCAGCTGAA GAAGGAGCAC 
CATAGACAAG AGCTTCACAT TAGAGCTTTG GAGTCCCAGA AGAGACAGCA AGAGATAGTG 
CTGAGACGCA AGACCCAAGA GGTTTCTGCT CTGCGGCGTC TCTCTAGGCG ACAAACTGAT 
CGATCTGGGG GACGAAGCCA CTCTGTTGCA GACTCTGGAG CAGAGTTGTC CACAAGCACA 
GCATCTTCAG AACCAGATAG TTCTTTACGT TCTGTGTCCA GTATTGTCCG TCATTGGAAC 
CGAAAGACAA GTGGAGACGT TGGGCCAGTG CCCAATGGGA GTCGTACACC AAGAAGGAAA 
GGGGTACGTC CAGGCCTACC ACTCAGTAAG GCTGCTCACA TGAAGTGGCA AACTCTGGAG 
AGAAGGGTTC ATGAAGTCGT CCTACAGAGA CTTACGCTTC TTAACTTAGA AAGCGATATG 
GAAAGACTGT TGCGGAAACG TGAGGAGCTG TCACTTCTGC AGGAAGCTTT GATAAGTCGT 
TCCATAGCTC TCCCAGAAGA CAGGAAAGCA GAGGAAAAGT CACAGCAGGA TGTTGCCGAA 
GAACTGGAAG CTTTGACAGC CAATATTGAT TATGTTAATG ACTGCATTCG AGAATGCCAG 
GCTACCATTG TTCAGATGGA GGAAACAAAG GAGGATCTAG ACTCAACAGA CACCTCAGTT 
GTGATCAGTT CTTGTTCTCT AACTGAGGCA CGACATCTTC TTGATAATTT CCTGAGAGCC 
TCAATAGATA AGGGCCTGCA AGTAGCTCAG AAAGAAGCCC AGATACGGAT TCTGGAAGCC 
CGGATTCGGC AAACTGACAT GGCTGGCCTG TCACAGGCAC ACATGCTGCT GGAGAAATCA 
GAGTGCCATT CTGTGCAGCA GGCAGAAAAC GGGTATGCTA GCACAGATGA AGAGGTGTCA 
GAAATTAGCC AGACTTCTGA TGGAAGTGCT TCTTTCTCCT CCATGAAAGG ATCCTGCAGT 
CAAGATGACT TTAAGTGGAA GGGGGAACCA CGGCTTTCAT GGCAAGTGAA GGCCGTGTCT 
GCTGAGTCTT TGAGTCCGCT GCTTGAAGCT TCCACTAGAA ACATCACCAA ATCCTTAGCA 
TCATTAACTG ACATCCTAGA AGATGTTGGG GGATTCTCTC CGAGTGGTTT GCGGGGCAGA 
GCATCACACA GCCTTACTCT TCCAATACGT GGTAATACAT TTCCGAGACA ATCCAGAGGT 
TTCAACACAT CCCCTGTGAC ACGCAGAAAA TCATATGACA GAGGACAGCC AAGGGCTTCA 
GACTCAAACC TAACACCTCC ATCATCTCCC CCTATGCGCC CTCGTAATGA TAAAAATGTC 
TTTTCTCGGC TAACAAGCAG TCAGAGTTCA GGCACTGCAC TGGATAAGTC TGATGAGAGT 
GACAGCTCTT TCTCAGATGT CTCCAGGGGC ATAATAAGTC AATTTGGAGG GCTAAAGAGC 
ATGAGGACAG CTCCTTTGCA GTGTGTTGCA ATTGCTGAGG GACATACAAA GCCAGTTCTT 
TGTGTTGAAG CTACAGATGA GCTCCTCTTT ACAGGATCTA AAGATCGCAC ATGCAAACTC 
TGGAATCTGG TAACAGGGCA GGAAATTGCA TCCCTTCGCA ACCATCCCCA TAACGTTGTA 
TCTCTGAAAC ATTGCCCAAA CTCTGGGCTA GTTCTCACAG CATCTGCTTC TTGTGTGCGA 
GTATGGGATA TCCGTGACAG TGCTCGCTGT GTGAGGACAT TGTTGTTTCA ACCCATTGGA 
AAGCTAACAG GACACATGGC TCCAGTCATG TGTCTTACAG TGAGCAACAC ACAAAACGGA 
CGAGATCTTG TTATCACAGG ATCTAAAGAT CACTATGTCA AGATATTTGA ACTCCATTCT 
GGATGGGGAG GGGCAGTTCC CCCCACTTAT AACCTGGAAC CTCCTCATTA CGATGGTATT 
GAGTGCTTGG CTGTACAGAA TGGGATCCTT TGCAGTGGCT CTCGAGATAA TGGAATCAAA 
AAGTGGGATC TACAGCAACA TGAGCTCACT CAGCAAGTGC CAAATGCACA CAGAGACTGG 
ATTTGTGCTT TAGGATTTGT ACCGGGACGT TCAATGTTGC TTAGTGGATG TCGTGGTGGC 
ATTCTAAAGG TTTGGAACAT GGAGAACTTT ACACCTATTG GGGAGATCAA AGGCCACGAA 
AGCCCCATTA ATGCGATGTG TTCTAATAGC CGTCACATCT TCACTGCATC TAGTGACTGC 
CGAGTCAAGC TATGGGTTTA TGTTCCTGGT CTTACTCCAT GTCTCCCTCG ACGTGTTCTG 
GCAATCAAGG GCCGTGCCAC TAGCCTTCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kif21b
Entrez Gene ID	779607
Full Name	kinesin family member 21B
Synonyms	kif21a
General protein information	Preferred Names kinesin family member 21B Names kinesin-like protein KIF21B kinesin family member 21A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Gallus gallus (chicken)	KIF21B	XP_004934872.1
Danio rerio (zebrafish)	kif21b	XP_699081.6
Homo sapiens (human)	KIF21B	NP_001239029.1
Rattus norvegicus (Norway rat)	Kif21b	NP_001099460.1
Pan troglodytes (chimpanzee)	KIF21B	XP_003308718.2
Xenopus tropicalis (tropical clawed frog)	kif21b	XP_004910777.1
Canis lupus familiaris (dog)	KIF21B	XP_547366.4
Mus musculus (house mouse)	Kif21b	NP_001034561.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kif21b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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