frmd4a gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018092333.1	XP_017947822.1	FERM domain-containing protein 4A isoform X3
XM_012960028.2	XP_012815482.1	FERM domain-containing protein 4A isoform X4
XM_018092333.2	XP_017947822.1	FERM domain-containing protein 4A isoform X5
XM_012960029.3	XP_012815483.1	FERM domain-containing protein 4A isoform X6
XM_031899042.1	XP_031754902.1	FERM domain-containing protein 4A isoform X4
XM_012960028.3	XP_012815482.1	FERM domain-containing protein 4A isoform X6
XM_002935243.4	XP_002935289.2	FERM domain-containing protein 4A isoform X1
XM_012960029.2	XP_012815483.1	FERM domain-containing protein 4A isoform X4
XM_002935243.5	XP_002935289.2	FERM domain-containing protein 4A isoform X1
XM_031899041.1	XP_031754901.1	FERM domain-containing protein 4A isoform X2
XM_012960027.2	XP_012815481.1	FERM domain-containing protein 4A isoform X2
XM_012960027.3	XP_012815481.1	FERM domain-containing protein 4A isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07920	XM_002935243.5 Latest version!	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa28647	XM_012960027.3 Latest version!	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa28648	XM_012960029.3 Latest version!	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa28648	XM_012960028.3 Latest version!	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa43298	XM_018092333.2 Latest version!	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa54883	XM_031899041.1 Latest version!	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa54884	XM_031899042.1 Latest version!	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa43298	XM_018092333.1	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa28648	XM_012960028.2	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa28648	XM_012960029.2	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa07920	XM_002935243.4	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa28647	XM_012960027.2	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

frmd4a ( XM_002935243.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935289.2 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07920
Clone ID Related Accession (Same CDS sequence)	XM_002935243.4 , XM_002935243.5
Accession Version	XM_002935243.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935243.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935289.2
CDS	26..3283
Translation	MRKLEEPAAAGDCRGSPALLPELSQPQADMVLQTAVTPGRARRLLFRIPYGSLRRRSGERMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_002935243.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935243.5

ORF Insert Sequence:

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_012960027.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815481.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa28647
Clone ID Related Accession (Same CDS sequence)	XM_012960027.2 , XM_012960027.3
Accession Version	XM_012960027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGGAGG CAACAGAAAA CTCACCCACG 
CTGGATGGGT CTGAGTCTTC CAGTCACCAA AGTACTGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815481.1
CDS	30..3254
Translation	MRKLEEPAAAGDCRGSPALLPELSQPQADMVLQTAVTPGRARRLLFRIPYGSLRRRSGERMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGEATENSPTLDGSESSSHQSTDE

Target ORF information:

RefSeq Version	XM_012960027.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960027.3

ORF Insert Sequence:

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGGAGG CAACAGAAAA CTCACCCACG 
CTGGATGGGT CTGAGTCTTC CAGTCACCAA AGTACTGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_012960029.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815483.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa28648
Clone ID Related Accession (Same CDS sequence)	XM_012960028.2 , XM_012960029.3 , XM_012960028.3 , XM_012960029.2
Accession Version	XM_012960029.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815483.1
CDS	369..3446
Translation	MTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_012960029.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960029.3

ORF Insert Sequence:

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_012960028.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815482.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa28648
Clone ID Related Accession (Same CDS sequence)	XM_012960028.2 , XM_012960029.3 , XM_012960028.3 , XM_012960029.2
Accession Version	XM_012960028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012960028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815482.1
CDS	380..3457
Translation	MTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_012960028.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960028.3

ORF Insert Sequence:

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_018092333.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947822.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X3, mRNA.

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CloneID	OXa43298
Clone ID Related Accession (Same CDS sequence)	XM_018092333.1 , XM_018092333.2
Accession Version	XM_018092333.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC TCCTGTCTCC AATGAGAGCC AAGATGACGG AGGGGCGGCG ATGTCAAGTT 
CACCTCCTAG ATGACAGGAA GCTGGAACTT CTTGTTCAGC CAAAGCTATT GGCCAAGGAG 
TTGCTTGATC TTGTTGCATC ACACTTCAAT CTGAAAGAAA AGGAATATTT TGGGATAGCC 
TTTACAGATG AAACGGGCCA TTTGAATTGG TTGCAGTTGG ACCGCAGAGT ACTAGAACAC 
GACTTTCCCA AAAAGTCTGG TCCGATTGTG CTGTACTTTT GTGTAAGGTT TTACATAGAA 
AGTATATCAT ACCTGAAGGA CAATGCGACC ATTGAACTGT TCTTCCTTAA TGCCAAGTCA 
TGTATATACA AGGAGTTAAT AGATGTTGAC AGTGAAGTTG TTTTTGAATT AGCTGCGTAT 
ATCCTGCAGG AAGCAAAGGG AGATTTTACA AGTGATGATT TTACAAGAGC AGACTTAAAG 
AAGTTACCAG CTCTTCCAAC TCAGGCCCTG AAGGAGCACC CTTCACTTGC ATACTGTGAG 
GACAGAGTAA TAGAATACTA CAAGAAACTG AATGGCCAAA CAAGAGGACA AGCTATTGTG 
AATTATATGA GTATTGTGGA GTCGCTTCCC ACATATGGAG TTCATTATTA TGCAGTCAAG 
GACAAGCAAG GAATCCCCTG GTGGTTGGGG TTAAGTTACA AAGGTATTTT CCAATATGAC 
CATCATGATA AGGTGAAACC ACGAAAGATC TTTCAATGGC GGCAGCTGGA AAACCTTTAC 
TTCAGGGAGA AGAAGTTTTC GGTAGAGGTT CATGACCCTC GCAGGGCCTC TGTTACCAGA 
AGGACATTTG GACATGGTGG AATTGCAGTG CACACATGGT ACGCATGCGC AGCCCTTATA 
AAGTCCATCT GGGCTATGGC CATTAGCCAA CATCAATTCT ATCTTGATAG GAAGCAAAGC 
AAGTCAAAAA TACATGCTGC AAGGAGCCTG AGTGAGATTG CTATTGACCT GACAGAGACA 
GGAACCCTAA AGACTTCAAA ACTTGCCAAT ATGGGCAGCA AAGGAAAAAT AATCAGCGGA 
AGCAGTGGGA GTCTGTTATC CTCTGGTTCC CAAGAGTCCG ATAGTTCGCA GTCTGCCAAG 
AAAGACATGC TGGCTGCACT GAAATCCAGG CAGGAAGCTC TGGAGGAGAC ACTACGCCAG 
CGCATAGAAG AGCTGAAGAA ACTGTGTGTG CGGGAAGCGG AGCTCACAGG TAAATTGCCA 
AGAGAGTATC CTTTAGATCC TGGGGAAGAG CCCCCTACAG TACGAAGACG AATAGGGACA 
TCATTTAAGC TTGATGAGCA GAAACTTCTA GCAAAAGGAG AGGAGGTCGA GCTGGAACGT 
CTGGAGAGGG AATTTGCAAT CCAGTCACAA ATCACAGAGG CGGCTCGTCG CTTGGCCAGT 
GATCCACACG TCAGCAGGAA GCTGAAAAAA CAACGAAAGA CCTCATATCT TAACGCACTA 
AAAAAACTGC AGGAGATTGA AAACTCAATA AATGAGTACC GCATCAAATC TGGGAAAAAG 
CCCACGCAGA GAGCTTCCCT GATTATAGAT GAAGCCAATC TCGGCAGTGA AGACAGCTCC 
CTCTCTGATG CTCTTGTGCT TGAAGATGAA GACTCTCAGG TTCCCAGCAT ACTGTCTCCT 
ATGCAGTCAC CCCACAAAGG TTTACCACCA AGGCCCCCAT CGCATGCCAG ACCCCACCCT 
CCACAGTCAC TGGATGGACT GAGACAGTTG CATTACCAGA GAAATGATTA TGACAAGTCA 
CCCATAAAGC CCAAAATGTG GAGTGAATCC TCCTTGGATG AACCATATGA GAAATTAAAA 
AAAAGATCAT CACACAGTCA TTCCAGTCAC CACAAAAGGT TTCCTAGCAC AGGAAGTTGT 
TCTGAGGCCG GGGGAAGCAG TTCCCTACAG AATAGCCCAA TACGAACACT GCCTCACTGG 
AATTCCCAGT CCAGCATGCC CTCCACACCA GACCTGCGAG TACGCAGCCC ACACTATGTA 
CATTCAACCA GGTCAGTGGA CATCAGCCCC ACCAGGCTGC ACAGCCTAGC ACAGCACTTT 
AGACACCGGA GCTCAAGTTT GGAGTCACAG GGAAAGCTTT TGGGCTCTGA GAATGACACT 
GGGAGCCCAG ATTTCTACAC CCCAAGGACT CGTAGTAGTA ATGGCTCTGA TCCCATGGAT 
GACTGTTCCT CCTGTACAAG CCATTCCAGC TCAGAACACT ACTACCCAAC TCAGATGAAC 
CCTAATTACT CAACATTGGC TGAAGATTCC CCCTCCAAGG CCAGGGAACG TCAGCGACAT 
AAATATAAAT CTAATGGCAA CCTTGTATCC TCCAATTCAG GGAGTATGCC CAACTTAGCT 
GCCAGAAATG GTACAGGGCA TCACGGAGTC TACCTTCATA GCCAGAGCCA GCCTTCATCT 
CAGTACAGGA TCAAAGAGTA TCCACTTTAC ATTGAAGGAA GCTCATCGCC TGTGGTTGTG 
CGCAGTCTAG AGAATGACCA AGAGGGACAC TACAGTGTCA AAGCACAATT CAAGACCTCT 
AACTCTTACA CTGCAGGAGG GATGTACAAA GAGAACTGGC ATGGTGATGA TTCAGTAGAT 
TCTGTGAGGC TTACTCCCTC CCGCTCTCAG ATTGTAAGAA CTCCATCACT TGGGAGAGAG 
GGCTCTGAGA AGGGCCCAGG CAGGACTGTG GTTTCTAATG AACTGCGCCA GTGGTACCAC 
AGGTCTACGT CATCTCACAA AGAGCACAAC AGACTTTCAC ACACTAGTTC GAATTCCTCT 
GACAGTGGCT CCCAGTACAG CACATCTCAA AGCACCTTTG TGGCACACAG TAGGGTAACA 
AGAATGCCTC CAGTGTGTAA AGCAACAACA GCTGCCTTAC CTCATGGTCA GAGGAGTTCA 
ACGCCTTGTA GTGATCTCAC AGCCACGCCT CCAAGTAGCC CTCACCACAT TCTATCATGG 
CAGACTGGGA GCTACAATGA TAGCTTCTAC CTAGACTCTG CTCTGTATCA AGAATTAGCT 
GATGTCCAGT GGTACGGACA AGAGAAAGCC AAGCCTGGGA CTCTTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947822.1
CDS	189..3299
Translation	MEGLLSPMRAKMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_018092333.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092333.1

ORF Insert Sequence:

ATGGAAGGAC TCCTGTCTCC AATGAGAGCC AAGATGACGG AGGGGCGGCG ATGTCAAGTT 
CACCTCCTAG ATGACAGGAA GCTGGAACTT CTTGTTCAGC CAAAGCTATT GGCCAAGGAG 
TTGCTTGATC TTGTTGCATC ACACTTCAAT CTGAAAGAAA AGGAATATTT TGGGATAGCC 
TTTACAGATG AAACGGGCCA TTTGAATTGG TTGCAGTTGG ACCGCAGAGT ACTAGAACAC 
GACTTTCCCA AAAAGTCTGG TCCGATTGTG CTGTACTTTT GTGTAAGGTT TTACATAGAA 
AGTATATCAT ACCTGAAGGA CAATGCGACC ATTGAACTGT TCTTCCTTAA TGCCAAGTCA 
TGTATATACA AGGAGTTAAT AGATGTTGAC AGTGAAGTTG TTTTTGAATT AGCTGCGTAT 
ATCCTGCAGG AAGCAAAGGG AGATTTTACA AGTGATGATT TTACAAGAGC AGACTTAAAG 
AAGTTACCAG CTCTTCCAAC TCAGGCCCTG AAGGAGCACC CTTCACTTGC ATACTGTGAG 
GACAGAGTAA TAGAATACTA CAAGAAACTG AATGGCCAAA CAAGAGGACA AGCTATTGTG 
AATTATATGA GTATTGTGGA GTCGCTTCCC ACATATGGAG TTCATTATTA TGCAGTCAAG 
GACAAGCAAG GAATCCCCTG GTGGTTGGGG TTAAGTTACA AAGGTATTTT CCAATATGAC 
CATCATGATA AGGTGAAACC ACGAAAGATC TTTCAATGGC GGCAGCTGGA AAACCTTTAC 
TTCAGGGAGA AGAAGTTTTC GGTAGAGGTT CATGACCCTC GCAGGGCCTC TGTTACCAGA 
AGGACATTTG GACATGGTGG AATTGCAGTG CACACATGGT ACGCATGCGC AGCCCTTATA 
AAGTCCATCT GGGCTATGGC CATTAGCCAA CATCAATTCT ATCTTGATAG GAAGCAAAGC 
AAGTCAAAAA TACATGCTGC AAGGAGCCTG AGTGAGATTG CTATTGACCT GACAGAGACA 
GGAACCCTAA AGACTTCAAA ACTTGCCAAT ATGGGCAGCA AAGGAAAAAT AATCAGCGGA 
AGCAGTGGGA GTCTGTTATC CTCTGGTTCC CAAGAGTCCG ATAGTTCGCA GTCTGCCAAG 
AAAGACATGC TGGCTGCACT GAAATCCAGG CAGGAAGCTC TGGAGGAGAC ACTACGCCAG 
CGCATAGAAG AGCTGAAGAA ACTGTGTGTG CGGGAAGCGG AGCTCACAGG TAAATTGCCA 
AGAGAGTATC CTTTAGATCC TGGGGAAGAG CCCCCTACAG TACGAAGACG AATAGGGACA 
TCATTTAAGC TTGATGAGCA GAAACTTCTA GCAAAAGGAG AGGAGGTCGA GCTGGAACGT 
CTGGAGAGGG AATTTGCAAT CCAGTCACAA ATCACAGAGG CGGCTCGTCG CTTGGCCAGT 
GATCCACACG TCAGCAGGAA GCTGAAAAAA CAACGAAAGA CCTCATATCT TAACGCACTA 
AAAAAACTGC AGGAGATTGA AAACTCAATA AATGAGTACC GCATCAAATC TGGGAAAAAG 
CCCACGCAGA GAGCTTCCCT GATTATAGAT GAAGCCAATC TCGGCAGTGA AGACAGCTCC 
CTCTCTGATG CTCTTGTGCT TGAAGATGAA GACTCTCAGG TTCCCAGCAT ACTGTCTCCT 
ATGCAGTCAC CCCACAAAGG TTTACCACCA AGGCCCCCAT CGCATGCCAG ACCCCACCCT 
CCACAGTCAC TGGATGGACT GAGACAGTTG CATTACCAGA GAAATGATTA TGACAAGTCA 
CCCATAAAGC CCAAAATGTG GAGTGAATCC TCCTTGGATG AACCATATGA GAAATTAAAA 
AAAAGATCAT CACACAGTCA TTCCAGTCAC CACAAAAGGT TTCCTAGCAC AGGAAGTTGT 
TCTGAGGCCG GGGGAAGCAG TTCCCTACAG AATAGCCCAA TACGAACACT GCCTCACTGG 
AATTCCCAGT CCAGCATGCC CTCCACACCA GACCTGCGAG TACGCAGCCC ACACTATGTA 
CATTCAACCA GGTCAGTGGA CATCAGCCCC ACCAGGCTGC ACAGCCTAGC ACAGCACTTT 
AGACACCGGA GCTCAAGTTT GGAGTCACAG GGAAAGCTTT TGGGCTCTGA GAATGACACT 
GGGAGCCCAG ATTTCTACAC CCCAAGGACT CGTAGTAGTA ATGGCTCTGA TCCCATGGAT 
GACTGTTCCT CCTGTACAAG CCATTCCAGC TCAGAACACT ACTACCCAAC TCAGATGAAC 
CCTAATTACT CAACATTGGC TGAAGATTCC CCCTCCAAGG CCAGGGAACG TCAGCGACAT 
AAATATAAAT CTAATGGCAA CCTTGTATCC TCCAATTCAG GGAGTATGCC CAACTTAGCT 
GCCAGAAATG GTACAGGGCA TCACGGAGTC TACCTTCATA GCCAGAGCCA GCCTTCATCT 
CAGTACAGGA TCAAAGAGTA TCCACTTTAC ATTGAAGGAA GCTCATCGCC TGTGGTTGTG 
CGCAGTCTAG AGAATGACCA AGAGGGACAC TACAGTGTCA AAGCACAATT CAAGACCTCT 
AACTCTTACA CTGCAGGAGG GATGTACAAA GAGAACTGGC ATGGTGATGA TTCAGTAGAT 
TCTGTGAGGC TTACTCCCTC CCGCTCTCAG ATTGTAAGAA CTCCATCACT TGGGAGAGAG 
GGCTCTGAGA AGGGCCCAGG CAGGACTGTG GTTTCTAATG AACTGCGCCA GTGGTACCAC 
AGGTCTACGT CATCTCACAA AGAGCACAAC AGACTTTCAC ACACTAGTTC GAATTCCTCT 
GACAGTGGCT CCCAGTACAG CACATCTCAA AGCACCTTTG TGGCACACAG TAGGGTAACA 
AGAATGCCTC CAGTGTGTAA AGCAACAACA GCTGCCTTAC CTCATGGTCA GAGGAGTTCA 
ACGCCTTGTA GTGATCTCAC AGCCACGCCT CCAAGTAGCC CTCACCACAT TCTATCATGG 
CAGACTGGGA GCTACAATGA TAGCTTCTAC CTAGACTCTG CTCTGTATCA AGAATTAGCT 
GATGTCCAGT GGTACGGACA AGAGAAAGCC AAGCCTGGGA CTCTTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_018092333.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947822.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa43298
Clone ID Related Accession (Same CDS sequence)	XM_018092333.1 , XM_018092333.2
Accession Version	XM_018092333.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC TCCTGTCTCC AATGAGAGCC AAGATGACGG AGGGGCGGCG ATGTCAAGTT 
CACCTCCTAG ATGACAGGAA GCTGGAACTT CTTGTTCAGC CAAAGCTATT GGCCAAGGAG 
TTGCTTGATC TTGTTGCATC ACACTTCAAT CTGAAAGAAA AGGAATATTT TGGGATAGCC 
TTTACAGATG AAACGGGCCA TTTGAATTGG TTGCAGTTGG ACCGCAGAGT ACTAGAACAC 
GACTTTCCCA AAAAGTCTGG TCCGATTGTG CTGTACTTTT GTGTAAGGTT TTACATAGAA 
AGTATATCAT ACCTGAAGGA CAATGCGACC ATTGAACTGT TCTTCCTTAA TGCCAAGTCA 
TGTATATACA AGGAGTTAAT AGATGTTGAC AGTGAAGTTG TTTTTGAATT AGCTGCGTAT 
ATCCTGCAGG AAGCAAAGGG AGATTTTACA AGTGATGATT TTACAAGAGC AGACTTAAAG 
AAGTTACCAG CTCTTCCAAC TCAGGCCCTG AAGGAGCACC CTTCACTTGC ATACTGTGAG 
GACAGAGTAA TAGAATACTA CAAGAAACTG AATGGCCAAA CAAGAGGACA AGCTATTGTG 
AATTATATGA GTATTGTGGA GTCGCTTCCC ACATATGGAG TTCATTATTA TGCAGTCAAG 
GACAAGCAAG GAATCCCCTG GTGGTTGGGG TTAAGTTACA AAGGTATTTT CCAATATGAC 
CATCATGATA AGGTGAAACC ACGAAAGATC TTTCAATGGC GGCAGCTGGA AAACCTTTAC 
TTCAGGGAGA AGAAGTTTTC GGTAGAGGTT CATGACCCTC GCAGGGCCTC TGTTACCAGA 
AGGACATTTG GACATGGTGG AATTGCAGTG CACACATGGT ACGCATGCGC AGCCCTTATA 
AAGTCCATCT GGGCTATGGC CATTAGCCAA CATCAATTCT ATCTTGATAG GAAGCAAAGC 
AAGTCAAAAA TACATGCTGC AAGGAGCCTG AGTGAGATTG CTATTGACCT GACAGAGACA 
GGAACCCTAA AGACTTCAAA ACTTGCCAAT ATGGGCAGCA AAGGAAAAAT AATCAGCGGA 
AGCAGTGGGA GTCTGTTATC CTCTGGTTCC CAAGAGTCCG ATAGTTCGCA GTCTGCCAAG 
AAAGACATGC TGGCTGCACT GAAATCCAGG CAGGAAGCTC TGGAGGAGAC ACTACGCCAG 
CGCATAGAAG AGCTGAAGAA ACTGTGTGTG CGGGAAGCGG AGCTCACAGG TAAATTGCCA 
AGAGAGTATC CTTTAGATCC TGGGGAAGAG CCCCCTACAG TACGAAGACG AATAGGGACA 
TCATTTAAGC TTGATGAGCA GAAACTTCTA GCAAAAGGAG AGGAGGTCGA GCTGGAACGT 
CTGGAGAGGG AATTTGCAAT CCAGTCACAA ATCACAGAGG CGGCTCGTCG CTTGGCCAGT 
GATCCACACG TCAGCAGGAA GCTGAAAAAA CAACGAAAGA CCTCATATCT TAACGCACTA 
AAAAAACTGC AGGAGATTGA AAACTCAATA AATGAGTACC GCATCAAATC TGGGAAAAAG 
CCCACGCAGA GAGCTTCCCT GATTATAGAT GAAGCCAATC TCGGCAGTGA AGACAGCTCC 
CTCTCTGATG CTCTTGTGCT TGAAGATGAA GACTCTCAGG TTCCCAGCAT ACTGTCTCCT 
ATGCAGTCAC CCCACAAAGG TTTACCACCA AGGCCCCCAT CGCATGCCAG ACCCCACCCT 
CCACAGTCAC TGGATGGACT GAGACAGTTG CATTACCAGA GAAATGATTA TGACAAGTCA 
CCCATAAAGC CCAAAATGTG GAGTGAATCC TCCTTGGATG AACCATATGA GAAATTAAAA 
AAAAGATCAT CACACAGTCA TTCCAGTCAC CACAAAAGGT TTCCTAGCAC AGGAAGTTGT 
TCTGAGGCCG GGGGAAGCAG TTCCCTACAG AATAGCCCAA TACGAACACT GCCTCACTGG 
AATTCCCAGT CCAGCATGCC CTCCACACCA GACCTGCGAG TACGCAGCCC ACACTATGTA 
CATTCAACCA GGTCAGTGGA CATCAGCCCC ACCAGGCTGC ACAGCCTAGC ACAGCACTTT 
AGACACCGGA GCTCAAGTTT GGAGTCACAG GGAAAGCTTT TGGGCTCTGA GAATGACACT 
GGGAGCCCAG ATTTCTACAC CCCAAGGACT CGTAGTAGTA ATGGCTCTGA TCCCATGGAT 
GACTGTTCCT CCTGTACAAG CCATTCCAGC TCAGAACACT ACTACCCAAC TCAGATGAAC 
CCTAATTACT CAACATTGGC TGAAGATTCC CCCTCCAAGG CCAGGGAACG TCAGCGACAT 
AAATATAAAT CTAATGGCAA CCTTGTATCC TCCAATTCAG GGAGTATGCC CAACTTAGCT 
GCCAGAAATG GTACAGGGCA TCACGGAGTC TACCTTCATA GCCAGAGCCA GCCTTCATCT 
CAGTACAGGA TCAAAGAGTA TCCACTTTAC ATTGAAGGAA GCTCATCGCC TGTGGTTGTG 
CGCAGTCTAG AGAATGACCA AGAGGGACAC TACAGTGTCA AAGCACAATT CAAGACCTCT 
AACTCTTACA CTGCAGGAGG GATGTACAAA GAGAACTGGC ATGGTGATGA TTCAGTAGAT 
TCTGTGAGGC TTACTCCCTC CCGCTCTCAG ATTGTAAGAA CTCCATCACT TGGGAGAGAG 
GGCTCTGAGA AGGGCCCAGG CAGGACTGTG GTTTCTAATG AACTGCGCCA GTGGTACCAC 
AGGTCTACGT CATCTCACAA AGAGCACAAC AGACTTTCAC ACACTAGTTC GAATTCCTCT 
GACAGTGGCT CCCAGTACAG CACATCTCAA AGCACCTTTG TGGCACACAG TAGGGTAACA 
AGAATGCCTC CAGTGTGTAA AGCAACAACA GCTGCCTTAC CTCATGGTCA GAGGAGTTCA 
ACGCCTTGTA GTGATCTCAC AGCCACGCCT CCAAGTAGCC CTCACCACAT TCTATCATGG 
CAGACTGGGA GCTACAATGA TAGCTTCTAC CTAGACTCTG CTCTGTATCA AGAATTAGCT 
GATGTCCAGT GGTACGGACA AGAGAAAGCC AAGCCTGGGA CTCTTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947822.1
CDS	203..3313
Translation	MEGLLSPMRAKMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_018092333.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092333.2

ORF Insert Sequence:

ATGGAAGGAC TCCTGTCTCC AATGAGAGCC AAGATGACGG AGGGGCGGCG ATGTCAAGTT 
CACCTCCTAG ATGACAGGAA GCTGGAACTT CTTGTTCAGC CAAAGCTATT GGCCAAGGAG 
TTGCTTGATC TTGTTGCATC ACACTTCAAT CTGAAAGAAA AGGAATATTT TGGGATAGCC 
TTTACAGATG AAACGGGCCA TTTGAATTGG TTGCAGTTGG ACCGCAGAGT ACTAGAACAC 
GACTTTCCCA AAAAGTCTGG TCCGATTGTG CTGTACTTTT GTGTAAGGTT TTACATAGAA 
AGTATATCAT ACCTGAAGGA CAATGCGACC ATTGAACTGT TCTTCCTTAA TGCCAAGTCA 
TGTATATACA AGGAGTTAAT AGATGTTGAC AGTGAAGTTG TTTTTGAATT AGCTGCGTAT 
ATCCTGCAGG AAGCAAAGGG AGATTTTACA AGTGATGATT TTACAAGAGC AGACTTAAAG 
AAGTTACCAG CTCTTCCAAC TCAGGCCCTG AAGGAGCACC CTTCACTTGC ATACTGTGAG 
GACAGAGTAA TAGAATACTA CAAGAAACTG AATGGCCAAA CAAGAGGACA AGCTATTGTG 
AATTATATGA GTATTGTGGA GTCGCTTCCC ACATATGGAG TTCATTATTA TGCAGTCAAG 
GACAAGCAAG GAATCCCCTG GTGGTTGGGG TTAAGTTACA AAGGTATTTT CCAATATGAC 
CATCATGATA AGGTGAAACC ACGAAAGATC TTTCAATGGC GGCAGCTGGA AAACCTTTAC 
TTCAGGGAGA AGAAGTTTTC GGTAGAGGTT CATGACCCTC GCAGGGCCTC TGTTACCAGA 
AGGACATTTG GACATGGTGG AATTGCAGTG CACACATGGT ACGCATGCGC AGCCCTTATA 
AAGTCCATCT GGGCTATGGC CATTAGCCAA CATCAATTCT ATCTTGATAG GAAGCAAAGC 
AAGTCAAAAA TACATGCTGC AAGGAGCCTG AGTGAGATTG CTATTGACCT GACAGAGACA 
GGAACCCTAA AGACTTCAAA ACTTGCCAAT ATGGGCAGCA AAGGAAAAAT AATCAGCGGA 
AGCAGTGGGA GTCTGTTATC CTCTGGTTCC CAAGAGTCCG ATAGTTCGCA GTCTGCCAAG 
AAAGACATGC TGGCTGCACT GAAATCCAGG CAGGAAGCTC TGGAGGAGAC ACTACGCCAG 
CGCATAGAAG AGCTGAAGAA ACTGTGTGTG CGGGAAGCGG AGCTCACAGG TAAATTGCCA 
AGAGAGTATC CTTTAGATCC TGGGGAAGAG CCCCCTACAG TACGAAGACG AATAGGGACA 
TCATTTAAGC TTGATGAGCA GAAACTTCTA GCAAAAGGAG AGGAGGTCGA GCTGGAACGT 
CTGGAGAGGG AATTTGCAAT CCAGTCACAA ATCACAGAGG CGGCTCGTCG CTTGGCCAGT 
GATCCACACG TCAGCAGGAA GCTGAAAAAA CAACGAAAGA CCTCATATCT TAACGCACTA 
AAAAAACTGC AGGAGATTGA AAACTCAATA AATGAGTACC GCATCAAATC TGGGAAAAAG 
CCCACGCAGA GAGCTTCCCT GATTATAGAT GAAGCCAATC TCGGCAGTGA AGACAGCTCC 
CTCTCTGATG CTCTTGTGCT TGAAGATGAA GACTCTCAGG TTCCCAGCAT ACTGTCTCCT 
ATGCAGTCAC CCCACAAAGG TTTACCACCA AGGCCCCCAT CGCATGCCAG ACCCCACCCT 
CCACAGTCAC TGGATGGACT GAGACAGTTG CATTACCAGA GAAATGATTA TGACAAGTCA 
CCCATAAAGC CCAAAATGTG GAGTGAATCC TCCTTGGATG AACCATATGA GAAATTAAAA 
AAAAGATCAT CACACAGTCA TTCCAGTCAC CACAAAAGGT TTCCTAGCAC AGGAAGTTGT 
TCTGAGGCCG GGGGAAGCAG TTCCCTACAG AATAGCCCAA TACGAACACT GCCTCACTGG 
AATTCCCAGT CCAGCATGCC CTCCACACCA GACCTGCGAG TACGCAGCCC ACACTATGTA 
CATTCAACCA GGTCAGTGGA CATCAGCCCC ACCAGGCTGC ACAGCCTAGC ACAGCACTTT 
AGACACCGGA GCTCAAGTTT GGAGTCACAG GGAAAGCTTT TGGGCTCTGA GAATGACACT 
GGGAGCCCAG ATTTCTACAC CCCAAGGACT CGTAGTAGTA ATGGCTCTGA TCCCATGGAT 
GACTGTTCCT CCTGTACAAG CCATTCCAGC TCAGAACACT ACTACCCAAC TCAGATGAAC 
CCTAATTACT CAACATTGGC TGAAGATTCC CCCTCCAAGG CCAGGGAACG TCAGCGACAT 
AAATATAAAT CTAATGGCAA CCTTGTATCC TCCAATTCAG GGAGTATGCC CAACTTAGCT 
GCCAGAAATG GTACAGGGCA TCACGGAGTC TACCTTCATA GCCAGAGCCA GCCTTCATCT 
CAGTACAGGA TCAAAGAGTA TCCACTTTAC ATTGAAGGAA GCTCATCGCC TGTGGTTGTG 
CGCAGTCTAG AGAATGACCA AGAGGGACAC TACAGTGTCA AAGCACAATT CAAGACCTCT 
AACTCTTACA CTGCAGGAGG GATGTACAAA GAGAACTGGC ATGGTGATGA TTCAGTAGAT 
TCTGTGAGGC TTACTCCCTC CCGCTCTCAG ATTGTAAGAA CTCCATCACT TGGGAGAGAG 
GGCTCTGAGA AGGGCCCAGG CAGGACTGTG GTTTCTAATG AACTGCGCCA GTGGTACCAC 
AGGTCTACGT CATCTCACAA AGAGCACAAC AGACTTTCAC ACACTAGTTC GAATTCCTCT 
GACAGTGGCT CCCAGTACAG CACATCTCAA AGCACCTTTG TGGCACACAG TAGGGTAACA 
AGAATGCCTC CAGTGTGTAA AGCAACAACA GCTGCCTTAC CTCATGGTCA GAGGAGTTCA 
ACGCCTTGTA GTGATCTCAC AGCCACGCCT CCAAGTAGCC CTCACCACAT TCTATCATGG 
CAGACTGGGA GCTACAATGA TAGCTTCTAC CTAGACTCTG CTCTGTATCA AGAATTAGCT 
GATGTCCAGT GGTACGGACA AGAGAAAGCC AAGCCTGGGA CTCTTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_031899041.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754901.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X2, mRNA.

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CloneID	OXa54883
Clone ID Related Accession (Same CDS sequence)	XM_031899041.1
Accession Version	XM_031899041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCG TTCTTCTAAG CTTTGAAGAT TTAATGTATA CTTGGGACAG CGTGGTATGG 
AACCTGACTT CACGGGCACT CCGTACATGT CGCTTTGGGA ACCTAAGAAT CCTGCAATTC 
GTGTTTAAGC CTTGGTGCAT TTCAAGGGCA GTATACCAGA TGACGGAGGG GCGGCGATGT 
CAAGTTCACC TCCTAGATGA CAGGAAGCTG GAACTTCTTG TTCAGCCAAA GCTATTGGCC 
AAGGAGTTGC TTGATCTTGT TGCATCACAC TTCAATCTGA AAGAAAAGGA ATATTTTGGG 
ATAGCCTTTA CAGATGAAAC GGGCCATTTG AATTGGTTGC AGTTGGACCG CAGAGTACTA 
GAACACGACT TTCCCAAAAA GTCTGGTCCG ATTGTGCTGT ACTTTTGTGT AAGGTTTTAC 
ATAGAAAGTA TATCATACCT GAAGGACAAT GCGACCATTG AACTGTTCTT CCTTAATGCC 
AAGTCATGTA TATACAAGGA GTTAATAGAT GTTGACAGTG AAGTTGTTTT TGAATTAGCT 
GCGTATATCC TGCAGGAAGC AAAGGGAGAT TTTACAAGTG ATGATTTTAC AAGAGCAGAC 
TTAAAGAAGT TACCAGCTCT TCCAACTCAG GCCCTGAAGG AGCACCCTTC ACTTGCATAC 
TGTGAGGACA GAGTAATAGA ATACTACAAG AAACTGAATG GCCAAACAAG AGGACAAGCT 
ATTGTGAATT ATATGAGTAT TGTGGAGTCG CTTCCCACAT ATGGAGTTCA TTATTATGCA 
GTCAAGGACA AGCAAGGAAT CCCCTGGTGG TTGGGGTTAA GTTACAAAGG TATTTTCCAA 
TATGACCATC ATGATAAGGT GAAACCACGA AAGATCTTTC AATGGCGGCA GCTGGAAAAC 
CTTTACTTCA GGGAGAAGAA GTTTTCGGTA GAGGTTCATG ACCCTCGCAG GGCCTCTGTT 
ACCAGAAGGA CATTTGGACA TGGTGGAATT GCAGTGCACA CATGGTACGC ATGCGCAGCC 
CTTATAAAGT CCATCTGGGC TATGGCCATT AGCCAACATC AATTCTATCT TGATAGGAAG 
CAAAGCAAGT CAAAAATACA TGCTGCAAGG AGCCTGAGTG AGATTGCTAT TGACCTGACA 
GAGACAGGAA CCCTAAAGAC TTCAAAACTT GCCAATATGG GCAGCAAAGG AAAAATAATC 
AGCGGAAGCA GTGGGAGTCT GTTATCCTCT GGTTCCCAAG AGTCCGATAG TTCGCAGTCT 
GCCAAGAAAG ACATGCTGGC TGCACTGAAA TCCAGGCAGG AAGCTCTGGA GGAGACACTA 
CGCCAGCGCA TAGAAGAGCT GAAGAAACTG TGTGTGCGGG AAGCGGAGCT CACAGGTAAA 
TTGCCAAGAG AGTATCCTTT AGATCCTGGG GAAGAGCCCC CTACAGTACG AAGACGAATA 
GGGACATCAT TTAAGCTTGA TGAGCAGAAA CTTCTAGCAA AAGGAGAGGA GGTCGAGCTG 
GAACGTCTGG AGAGGGAATT TGCAATCCAG TCACAAATCA CAGAGGCGGC TCGTCGCTTG 
GCCAGTGATC CACACGTCAG CAGGAAGCTG AAAAAACAAC GAAAGACCTC ATATCTTAAC 
GCACTAAAAA AACTGCAGGA GATTGAAAAC TCAATAAATG AGTACCGCAT CAAATCTGGG 
AAAAAGCCCA CGCAGAGAGC TTCCCTGATT ATAGATGAAG CCAATCTCGG CAGTGAAGAC 
AGCTCCCTCT CTGATGCTCT TGTGCTTGAA GATGAAGACT CTCAGGTTCC CAGCATACTG 
TCTCCTATGC AGTCACCCCA CAAAGGTTTA CCACCAAGGC CCCCATCGCA TGCCAGACCC 
CACCCTCCAC AGTCACTGGA TGGACTGAGA CAGTTGCATT ACCAGAGAAA TGATTATGAC 
AAGTCACCCA TAAAGCCCAA AATGTGGAGT GAATCCTCCT TGGATGAACC ATATGAGAAA 
TTAAAAAAAA GATCATCACA CAGTCATTCC AGTCACCACA AAAGGTTTCC TAGCACAGGA 
AGTTGTTCTG AGGCCGGGGG AAGCAGTTCC CTACAGAATA GCCCAATACG AACACTGCCT 
CACTGGAATT CCCAGTCCAG CATGCCCTCC ACACCAGACC TGCGAGTACG CAGCCCACAC 
TATGTACATT CAACCAGGTC AGTGGACATC AGCCCCACCA GGCTGCACAG CCTAGCACAG 
CACTTTAGAC ACCGGAGCTC AAGTTTGGAG TCACAGGGAA AGCTTTTGGG CTCTGAGAAT 
GACACTGGGA GCCCAGATTT CTACACCCCA AGGACTCGTA GTAGTAATGG CTCTGATCCC 
ATGGATGACT GTTCCTCCTG TACAAGCCAT TCCAGCTCAG AACACTACTA CCCAACTCAG 
ATGAACCCTA ATTACTCAAC ATTGGCTGAA GATTCCCCCT CCAAGGCCAG GGAACGTCAG 
CGACATAAAT ATAAATCTAA TGGCAACCTT GTATCCTCCA ATTCAGGGAG TATGCCCAAC 
TTAGCTGCCA GAAATGGTAC AGGGCATCAC GGAGTCTACC TTCATAGCCA GAGCCAGCCT 
TCATCTCAGT ACAGGATCAA AGAGTATCCA CTTTACATTG AAGGAAGCTC ATCGCCTGTG 
GTTGTGCGCA GTCTAGAGAA TGACCAAGAG GGACACTACA GTGTCAAAGC ACAATTCAAG 
ACCTCTAACT CTTACACTGC AGGAGGGATG TACAAAGAGA ACTGGCATGG TGATGATTCA 
GTAGATTCTG TGAGGCTTAC TCCCTCCCGC TCTCAGATTG TAAGAACTCC ATCACTTGGG 
AGAGAGGGCT CTGAGAAGGG CCCAGGCAGG ACTGTGGTTT CTAATGAACT GCGCCAGTGG 
TACCACAGGT CTACGTCATC TCACAAAGAG CACAACAGAC TTTCACACAC TAGTTCGAAT 
TCCTCTGACA GTGGCTCCCA GTACAGCACA TCTCAAAGCA CCTTTGTGGC ACACAGTAGG 
GTAACAAGAA TGCCTCCAGT GTGTAAAGCA ACAACAGCTG CCTTACCTCA TGGTCAGAGG 
AGTTCAACGC CTTGTAGTGA TCTCACAGCC ACGCCTCCAA GTAGCCCTCA CCACATTCTA 
TCATGGCAGA CTGGGAGCTA CAATGATAGC TTCTACCTAG ACTCTGCTCT GTATCAAGAA 
TTAGCTGATG TCCAGTGGTA CGGACAAGAG AAAGCCAAGC CTGGGACTCT TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754901.1
CDS	16..3252
Translation	MATVLLSFEDLMYTWDSVVWNLTSRALRTCRFGNLRILQFVFKPWCISRAVYQMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_031899041.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899041.1

ORF Insert Sequence:

ATGGCTACCG TTCTTCTAAG CTTTGAAGAT TTAATGTATA CTTGGGACAG CGTGGTATGG 
AACCTGACTT CACGGGCACT CCGTACATGT CGCTTTGGGA ACCTAAGAAT CCTGCAATTC 
GTGTTTAAGC CTTGGTGCAT TTCAAGGGCA GTATACCAGA TGACGGAGGG GCGGCGATGT 
CAAGTTCACC TCCTAGATGA CAGGAAGCTG GAACTTCTTG TTCAGCCAAA GCTATTGGCC 
AAGGAGTTGC TTGATCTTGT TGCATCACAC TTCAATCTGA AAGAAAAGGA ATATTTTGGG 
ATAGCCTTTA CAGATGAAAC GGGCCATTTG AATTGGTTGC AGTTGGACCG CAGAGTACTA 
GAACACGACT TTCCCAAAAA GTCTGGTCCG ATTGTGCTGT ACTTTTGTGT AAGGTTTTAC 
ATAGAAAGTA TATCATACCT GAAGGACAAT GCGACCATTG AACTGTTCTT CCTTAATGCC 
AAGTCATGTA TATACAAGGA GTTAATAGAT GTTGACAGTG AAGTTGTTTT TGAATTAGCT 
GCGTATATCC TGCAGGAAGC AAAGGGAGAT TTTACAAGTG ATGATTTTAC AAGAGCAGAC 
TTAAAGAAGT TACCAGCTCT TCCAACTCAG GCCCTGAAGG AGCACCCTTC ACTTGCATAC 
TGTGAGGACA GAGTAATAGA ATACTACAAG AAACTGAATG GCCAAACAAG AGGACAAGCT 
ATTGTGAATT ATATGAGTAT TGTGGAGTCG CTTCCCACAT ATGGAGTTCA TTATTATGCA 
GTCAAGGACA AGCAAGGAAT CCCCTGGTGG TTGGGGTTAA GTTACAAAGG TATTTTCCAA 
TATGACCATC ATGATAAGGT GAAACCACGA AAGATCTTTC AATGGCGGCA GCTGGAAAAC 
CTTTACTTCA GGGAGAAGAA GTTTTCGGTA GAGGTTCATG ACCCTCGCAG GGCCTCTGTT 
ACCAGAAGGA CATTTGGACA TGGTGGAATT GCAGTGCACA CATGGTACGC ATGCGCAGCC 
CTTATAAAGT CCATCTGGGC TATGGCCATT AGCCAACATC AATTCTATCT TGATAGGAAG 
CAAAGCAAGT CAAAAATACA TGCTGCAAGG AGCCTGAGTG AGATTGCTAT TGACCTGACA 
GAGACAGGAA CCCTAAAGAC TTCAAAACTT GCCAATATGG GCAGCAAAGG AAAAATAATC 
AGCGGAAGCA GTGGGAGTCT GTTATCCTCT GGTTCCCAAG AGTCCGATAG TTCGCAGTCT 
GCCAAGAAAG ACATGCTGGC TGCACTGAAA TCCAGGCAGG AAGCTCTGGA GGAGACACTA 
CGCCAGCGCA TAGAAGAGCT GAAGAAACTG TGTGTGCGGG AAGCGGAGCT CACAGGTAAA 
TTGCCAAGAG AGTATCCTTT AGATCCTGGG GAAGAGCCCC CTACAGTACG AAGACGAATA 
GGGACATCAT TTAAGCTTGA TGAGCAGAAA CTTCTAGCAA AAGGAGAGGA GGTCGAGCTG 
GAACGTCTGG AGAGGGAATT TGCAATCCAG TCACAAATCA CAGAGGCGGC TCGTCGCTTG 
GCCAGTGATC CACACGTCAG CAGGAAGCTG AAAAAACAAC GAAAGACCTC ATATCTTAAC 
GCACTAAAAA AACTGCAGGA GATTGAAAAC TCAATAAATG AGTACCGCAT CAAATCTGGG 
AAAAAGCCCA CGCAGAGAGC TTCCCTGATT ATAGATGAAG CCAATCTCGG CAGTGAAGAC 
AGCTCCCTCT CTGATGCTCT TGTGCTTGAA GATGAAGACT CTCAGGTTCC CAGCATACTG 
TCTCCTATGC AGTCACCCCA CAAAGGTTTA CCACCAAGGC CCCCATCGCA TGCCAGACCC 
CACCCTCCAC AGTCACTGGA TGGACTGAGA CAGTTGCATT ACCAGAGAAA TGATTATGAC 
AAGTCACCCA TAAAGCCCAA AATGTGGAGT GAATCCTCCT TGGATGAACC ATATGAGAAA 
TTAAAAAAAA GATCATCACA CAGTCATTCC AGTCACCACA AAAGGTTTCC TAGCACAGGA 
AGTTGTTCTG AGGCCGGGGG AAGCAGTTCC CTACAGAATA GCCCAATACG AACACTGCCT 
CACTGGAATT CCCAGTCCAG CATGCCCTCC ACACCAGACC TGCGAGTACG CAGCCCACAC 
TATGTACATT CAACCAGGTC AGTGGACATC AGCCCCACCA GGCTGCACAG CCTAGCACAG 
CACTTTAGAC ACCGGAGCTC AAGTTTGGAG TCACAGGGAA AGCTTTTGGG CTCTGAGAAT 
GACACTGGGA GCCCAGATTT CTACACCCCA AGGACTCGTA GTAGTAATGG CTCTGATCCC 
ATGGATGACT GTTCCTCCTG TACAAGCCAT TCCAGCTCAG AACACTACTA CCCAACTCAG 
ATGAACCCTA ATTACTCAAC ATTGGCTGAA GATTCCCCCT CCAAGGCCAG GGAACGTCAG 
CGACATAAAT ATAAATCTAA TGGCAACCTT GTATCCTCCA ATTCAGGGAG TATGCCCAAC 
TTAGCTGCCA GAAATGGTAC AGGGCATCAC GGAGTCTACC TTCATAGCCA GAGCCAGCCT 
TCATCTCAGT ACAGGATCAA AGAGTATCCA CTTTACATTG AAGGAAGCTC ATCGCCTGTG 
GTTGTGCGCA GTCTAGAGAA TGACCAAGAG GGACACTACA GTGTCAAAGC ACAATTCAAG 
ACCTCTAACT CTTACACTGC AGGAGGGATG TACAAAGAGA ACTGGCATGG TGATGATTCA 
GTAGATTCTG TGAGGCTTAC TCCCTCCCGC TCTCAGATTG TAAGAACTCC ATCACTTGGG 
AGAGAGGGCT CTGAGAAGGG CCCAGGCAGG ACTGTGGTTT CTAATGAACT GCGCCAGTGG 
TACCACAGGT CTACGTCATC TCACAAAGAG CACAACAGAC TTTCACACAC TAGTTCGAAT 
TCCTCTGACA GTGGCTCCCA GTACAGCACA TCTCAAAGCA CCTTTGTGGC ACACAGTAGG 
GTAACAAGAA TGCCTCCAGT GTGTAAAGCA ACAACAGCTG CCTTACCTCA TGGTCAGAGG 
AGTTCAACGC CTTGTAGTGA TCTCACAGCC ACGCCTCCAA GTAGCCCTCA CCACATTCTA 
TCATGGCAGA CTGGGAGCTA CAATGATAGC TTCTACCTAG ACTCTGCTCT GTATCAAGAA 
TTAGCTGATG TCCAGTGGTA CGGACAAGAG AAAGCCAAGC CTGGGACTCT TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_031899042.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031754902.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa54884
Clone ID Related Accession (Same CDS sequence)	XM_031899042.1
Accession Version	XM_031899042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCG TTCTTCTAAG CTTTGAAGAT TTAATGTATA CTTGGGACAG CGTGGTATGG 
AACCTGACTT CACGGGCACT CCGTACATGT CGCTTTGGGA ACCTAAGAAT CCTGCAATTC 
GTGTTTAAGC CTTGGTGCAT TTCAAGGGCA GTATACCAGA TGACGGAGGG GCGGCGATGT 
CAAGTTCACC TCCTAGATGA CAGGAAGCTG GAACTTCTTG TTCAGCCAAA GCTATTGGCC 
AAGGAGTTGC TTGATCTTGT TGCATCACAC TTCAATCTGA AAGAAAAGGA ATATTTTGGG 
ATAGCCTTTA CAGATGAAAC GGGCCATTTG AATTGGTTGC AGTTGGACCG CAGAGTACTA 
GAACACGACT TTCCCAAAAA GTCTGGTCCG ATTGTGCTGT ACTTTTGTGT AAGGTTTTAC 
ATAGAAAGTA TATCATACCT GAAGGACAAT GCGACCATTG AACTGTTCTT CCTTAATGCC 
AAGTCATGTA TATACAAGGA GTTAATAGAT GTTGACAGTG AAGTTGTTTT TGAATTAGCT 
GCGTATATCC TGCAGGAAGC AAAGGGAGAT TTTACAAGTG ATGATTTTAC AAGAGCAGAC 
TTAAAGAAGT TACCAGCTCT TCCAACTCAG GCCCTGAAGG AGCACCCTTC ACTTGCATAC 
TGTGAGGACA GAGTAATAGA ATACTACAAG AAACTGAATG GCCAAACAAG AGGACAAGCT 
ATTGTGAATT ATATGAGTAT TGTGGAGTCG CTTCCCACAT ATGGAGTTCA TTATTATGCA 
GTCAAGGACA AGCAAGGAAT CCCCTGGTGG TTGGGGTTAA GTTACAAAGG TATTTTCCAA 
TATGACCATC ATGATAAGGT GAAACCACGA AAGATCTTTC AATGGCGGCA GCTGGAAAAC 
CTTTACTTCA GGGAGAAGAA GTTTTCGGTA GAGGTTCATG ACCCTCGCAG GGCCTCTGTT 
ACCAGAAGGA CATTTGGACA TGGTGGAATT GCAGTGCACA CATGGTACGC ATGCGCAGCC 
CTTATAAAGT CCATCTGGGC TATGGCCATT AGCCAACATC AATTCTATCT TGATAGGAAG 
CAAAGCAAGT CAAAAATACA TGCTGCAAGG AGCCTGAGTG AGATTGCTAT TGACCTGACA 
GAGACAGGAA CCCTAAAGAC TTCAAAACTT GCCAATATGG GCAGCAAAGG AAAAATAATC 
AGCGGAAGCA GTGGGAGTCT GTTATCCTCT GGTTCCCAAG AGTCCGATAG TTCGCAGTCT 
GCCAAGAAAG ACATGCTGGC TGCACTGAAA TCCAGGCAGG AAGCTCTGGA GGAGACACTA 
CGCCAGCGCA TAGAAGAGCT GAAGAAACTG TGTGTGCGGG AAGCGGAGCT CACAGGTAAA 
TTGCCAAGAG AGTATCCTTT AGATCCTGGG GAAGAGCCCC CTACAGTACG AAGACGAATA 
GGGACATCAT TTAAGCTTGA TGAGCAGAAA CTTCTAGCAA AAGGAGAGGA GGTCGAGCTG 
GAACGTCTGG AGAGGGAATT TGCAATCCAG TCACAAATCA CAGAGGCGGC TCGTCGCTTG 
GCCAGTGATC CACACGTCAG CAGGAAGCTG AAAAAACAAC GAAAGACCTC ATATCTTAAC 
GCACTAAAAA AACTGCAGGA GATTGAAAAC TCAATAAATG AGTACCGCAT CAAATCTGGG 
AAAAAGCCCA CGCAGAGAGC TTCCCTGATT ATAGATGAAG CCAATCTCGG CAGTGAAGAC 
AGCTCCCTCT CTGATGCTCT TGTGCTTGAA GATGAAGACT CTCAGGTTCC CAGCATACTG 
TCTCCTATGC AGTCACCCCA CAAAGGTTTA CCACCAAGGC CCCCATCGCA TGCCAGACCC 
CACCCTCCAC AGTCACTGGA TGGACTGAGA CAGTTGCATT ACCAGAGAAA TGATTATGAC 
AAGTCACCCA TAAAGCCCAA AATGTGGAGT GAATCCTCCT TGGATGAACC ATATGAGAAA 
TTAAAAAAAA GATCATCACA CAGTCATTCC AGTCACCACA AAAGGTTTCC TAGCACAGGA 
AGTTGTTCTG AGGCCGGGGG AAGCAGTTCC CTACAGAATA GCCCAATACG AACACTGCCT 
CACTGGAATT CCCAGTCCAG CATGCCCTCC ACACCAGACC TGCGAGTACG CAGCCCACAC 
TATGTACATT CAACCAGGTC AGTGGACATC AGCCCCACCA GGCTGCACAG CCTAGCACAG 
CACTTTAGAC ACCGGAGCTC AAGTTTGGAG TCACAGGGAA AGCTTTTGGG CTCTGAGAAT 
GACACTGGGA GCCCAGATTT CTACACCCCA AGGACTCGTA GTAGTAATGG CTCTGATCCC 
ATGGATGACT GTTCCTCCTG TACAAGCCAT TCCAGCTCAG AACACTACTA CCCAACTCAG 
ATGAACCCTA ATTACTCAAC ATTGGCTGAA GATTCCCCCT CCAAGGCCAG GGAACGTCAG 
CGACATAAAT ATAAATCTAA TGGCAACCTT GTATCCTCCA ATTCAGGGAG TATGCCCAAC 
TTAGCTGCCA GAAATGGTAC AGGGCATCAC GGAGTCTACC TTCATAGCCA GAGCCAGCCT 
TCATCTCAGT ACAGGATCAA AGAGTATCCA CTTTACATTG AAGGAAGCTC ATCGCCTGTG 
GTTGTGCGCA GTCTAGAGAA TGACCAAGAG GGACACTACA GTGTCAAAGC ACAATTCAAG 
ACCTCTAACT CTTACACTGC AGGAGGGATG TACAAAGAGA ACTGGCATGG TGATGATTCA 
GTAGATTCTG TGAGGCTTAC TCCCTCCCGC TCTCAGATTG TAAGAACTCC ATCACTTGGG 
AGAGAGGGCT CTGAGAAGGG CCCAGGCAGG ACTGTGGTTT CTAATGAACT GCGCCAGTGG 
TACCACAGGT CTACGTCATC TCACAAAGAG CACAACAGAC TTTCACACAC TAGTTCGAAT 
TCCTCTGACA GTGGCTCCCA GTACAGCACA TCTCAAAGCA CCTTTGTGGC ACACAGTAGG 
GTAACAAGAA TGCCTCCAGT GTGTAAAGCA ACAACAGCTG CCTTACCTCA TGGTCAGAGG 
AGTTCAACGC CTTGTAGTGA TCTCACAGCC ACGCCTCCAA GTAGCCCTCA CCACATTCTA 
TCATGGCAGA CTGGGGAGGC AACAGAAAAC TCACCCACGC TGGATGGGTC TGAGTCTTCC 
AGTCACCAAA GTACTGATGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031754902.1
CDS	16..3219
Translation	MATVLLSFEDLMYTWDSVVWNLTSRALRTCRFGNLRILQFVFKPWCISRAVYQMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGEATENSPTLDGSESSSHQSTDE

Target ORF information:

RefSeq Version	XM_031899042.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899042.1

ORF Insert Sequence:

ATGGCTACCG TTCTTCTAAG CTTTGAAGAT TTAATGTATA CTTGGGACAG CGTGGTATGG 
AACCTGACTT CACGGGCACT CCGTACATGT CGCTTTGGGA ACCTAAGAAT CCTGCAATTC 
GTGTTTAAGC CTTGGTGCAT TTCAAGGGCA GTATACCAGA TGACGGAGGG GCGGCGATGT 
CAAGTTCACC TCCTAGATGA CAGGAAGCTG GAACTTCTTG TTCAGCCAAA GCTATTGGCC 
AAGGAGTTGC TTGATCTTGT TGCATCACAC TTCAATCTGA AAGAAAAGGA ATATTTTGGG 
ATAGCCTTTA CAGATGAAAC GGGCCATTTG AATTGGTTGC AGTTGGACCG CAGAGTACTA 
GAACACGACT TTCCCAAAAA GTCTGGTCCG ATTGTGCTGT ACTTTTGTGT AAGGTTTTAC 
ATAGAAAGTA TATCATACCT GAAGGACAAT GCGACCATTG AACTGTTCTT CCTTAATGCC 
AAGTCATGTA TATACAAGGA GTTAATAGAT GTTGACAGTG AAGTTGTTTT TGAATTAGCT 
GCGTATATCC TGCAGGAAGC AAAGGGAGAT TTTACAAGTG ATGATTTTAC AAGAGCAGAC 
TTAAAGAAGT TACCAGCTCT TCCAACTCAG GCCCTGAAGG AGCACCCTTC ACTTGCATAC 
TGTGAGGACA GAGTAATAGA ATACTACAAG AAACTGAATG GCCAAACAAG AGGACAAGCT 
ATTGTGAATT ATATGAGTAT TGTGGAGTCG CTTCCCACAT ATGGAGTTCA TTATTATGCA 
GTCAAGGACA AGCAAGGAAT CCCCTGGTGG TTGGGGTTAA GTTACAAAGG TATTTTCCAA 
TATGACCATC ATGATAAGGT GAAACCACGA AAGATCTTTC AATGGCGGCA GCTGGAAAAC 
CTTTACTTCA GGGAGAAGAA GTTTTCGGTA GAGGTTCATG ACCCTCGCAG GGCCTCTGTT 
ACCAGAAGGA CATTTGGACA TGGTGGAATT GCAGTGCACA CATGGTACGC ATGCGCAGCC 
CTTATAAAGT CCATCTGGGC TATGGCCATT AGCCAACATC AATTCTATCT TGATAGGAAG 
CAAAGCAAGT CAAAAATACA TGCTGCAAGG AGCCTGAGTG AGATTGCTAT TGACCTGACA 
GAGACAGGAA CCCTAAAGAC TTCAAAACTT GCCAATATGG GCAGCAAAGG AAAAATAATC 
AGCGGAAGCA GTGGGAGTCT GTTATCCTCT GGTTCCCAAG AGTCCGATAG TTCGCAGTCT 
GCCAAGAAAG ACATGCTGGC TGCACTGAAA TCCAGGCAGG AAGCTCTGGA GGAGACACTA 
CGCCAGCGCA TAGAAGAGCT GAAGAAACTG TGTGTGCGGG AAGCGGAGCT CACAGGTAAA 
TTGCCAAGAG AGTATCCTTT AGATCCTGGG GAAGAGCCCC CTACAGTACG AAGACGAATA 
GGGACATCAT TTAAGCTTGA TGAGCAGAAA CTTCTAGCAA AAGGAGAGGA GGTCGAGCTG 
GAACGTCTGG AGAGGGAATT TGCAATCCAG TCACAAATCA CAGAGGCGGC TCGTCGCTTG 
GCCAGTGATC CACACGTCAG CAGGAAGCTG AAAAAACAAC GAAAGACCTC ATATCTTAAC 
GCACTAAAAA AACTGCAGGA GATTGAAAAC TCAATAAATG AGTACCGCAT CAAATCTGGG 
AAAAAGCCCA CGCAGAGAGC TTCCCTGATT ATAGATGAAG CCAATCTCGG CAGTGAAGAC 
AGCTCCCTCT CTGATGCTCT TGTGCTTGAA GATGAAGACT CTCAGGTTCC CAGCATACTG 
TCTCCTATGC AGTCACCCCA CAAAGGTTTA CCACCAAGGC CCCCATCGCA TGCCAGACCC 
CACCCTCCAC AGTCACTGGA TGGACTGAGA CAGTTGCATT ACCAGAGAAA TGATTATGAC 
AAGTCACCCA TAAAGCCCAA AATGTGGAGT GAATCCTCCT TGGATGAACC ATATGAGAAA 
TTAAAAAAAA GATCATCACA CAGTCATTCC AGTCACCACA AAAGGTTTCC TAGCACAGGA 
AGTTGTTCTG AGGCCGGGGG AAGCAGTTCC CTACAGAATA GCCCAATACG AACACTGCCT 
CACTGGAATT CCCAGTCCAG CATGCCCTCC ACACCAGACC TGCGAGTACG CAGCCCACAC 
TATGTACATT CAACCAGGTC AGTGGACATC AGCCCCACCA GGCTGCACAG CCTAGCACAG 
CACTTTAGAC ACCGGAGCTC AAGTTTGGAG TCACAGGGAA AGCTTTTGGG CTCTGAGAAT 
GACACTGGGA GCCCAGATTT CTACACCCCA AGGACTCGTA GTAGTAATGG CTCTGATCCC 
ATGGATGACT GTTCCTCCTG TACAAGCCAT TCCAGCTCAG AACACTACTA CCCAACTCAG 
ATGAACCCTA ATTACTCAAC ATTGGCTGAA GATTCCCCCT CCAAGGCCAG GGAACGTCAG 
CGACATAAAT ATAAATCTAA TGGCAACCTT GTATCCTCCA ATTCAGGGAG TATGCCCAAC 
TTAGCTGCCA GAAATGGTAC AGGGCATCAC GGAGTCTACC TTCATAGCCA GAGCCAGCCT 
TCATCTCAGT ACAGGATCAA AGAGTATCCA CTTTACATTG AAGGAAGCTC ATCGCCTGTG 
GTTGTGCGCA GTCTAGAGAA TGACCAAGAG GGACACTACA GTGTCAAAGC ACAATTCAAG 
ACCTCTAACT CTTACACTGC AGGAGGGATG TACAAAGAGA ACTGGCATGG TGATGATTCA 
GTAGATTCTG TGAGGCTTAC TCCCTCCCGC TCTCAGATTG TAAGAACTCC ATCACTTGGG 
AGAGAGGGCT CTGAGAAGGG CCCAGGCAGG ACTGTGGTTT CTAATGAACT GCGCCAGTGG 
TACCACAGGT CTACGTCATC TCACAAAGAG CACAACAGAC TTTCACACAC TAGTTCGAAT 
TCCTCTGACA GTGGCTCCCA GTACAGCACA TCTCAAAGCA CCTTTGTGGC ACACAGTAGG 
GTAACAAGAA TGCCTCCAGT GTGTAAAGCA ACAACAGCTG CCTTACCTCA TGGTCAGAGG 
AGTTCAACGC CTTGTAGTGA TCTCACAGCC ACGCCTCCAA GTAGCCCTCA CCACATTCTA 
TCATGGCAGA CTGGGGAGGC AACAGAAAAC TCACCCACGC TGGATGGGTC TGAGTCTTCC 
AGTCACCAAA GTACTGATGA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_002935243.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935289.2 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07920
Clone ID Related Accession (Same CDS sequence)	XM_002935243.4 , XM_002935243.5
Accession Version	XM_002935243.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935243.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935289.2
CDS	44..3301
Translation	MRKLEEPAAAGDCRGSPALLPELSQPQADMVLQTAVTPGRARRLLFRIPYGSLRRRSGERMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_002935243.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935243.4

ORF Insert Sequence:

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_012960027.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815481.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa28647
Clone ID Related Accession (Same CDS sequence)	XM_012960027.2 , XM_012960027.3
Accession Version	XM_012960027.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGGAGG CAACAGAAAA CTCACCCACG 
CTGGATGGGT CTGAGTCTTC CAGTCACCAA AGTACTGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815481.1
CDS	52..3276
Translation	MRKLEEPAAAGDCRGSPALLPELSQPQADMVLQTAVTPGRARRLLFRIPYGSLRRRSGERMTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGEATENSPTLDGSESSSHQSTDE

Target ORF information:

RefSeq Version	XM_012960027.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960027.2

ORF Insert Sequence:

ATGAGAAAGC TGGAGGAGCC TGCTGCTGCT GGTGATTGCA GAGGAAGCCC TGCTCTGCTC 
CCCGAGCTCT CACAGCCACA GGCTGACATG GTGCTCCAGA CAGCAGTGAC TCCGGGCAGG 
GCCCGCAGAC TTTTGTTCAG AATACCATAT GGATCTCTGA GACGAAGAAG CGGAGAAAGG 
ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGGAGG CAACAGAAAA CTCACCCACG 
CTGGATGGGT CTGAGTCTTC CAGTCACCAA AGTACTGATG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_012960028.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815482.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa28648
Clone ID Related Accession (Same CDS sequence)	XM_012960028.2 , XM_012960029.3 , XM_012960028.3 , XM_012960029.2
Accession Version	XM_012960028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815482.1
CDS	446..3523
Translation	MTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_012960028.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960028.2

ORF Insert Sequence:

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

frmd4a ( XM_012960029.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815483.1 Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X5, mRNA.

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CloneID	OXa28648
Clone ID Related Accession (Same CDS sequence)	XM_012960028.2 , XM_012960029.3 , XM_012960028.3 , XM_012960029.2
Accession Version	XM_012960029.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	FERM domain-containing protein 4A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012960029.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815483.1
CDS	434..3511
Translation	MTEGRRCQVHLLDDRKLELLVQPKLLAKELLDLVASHFNLKEKEYFGIAFTDETGHLNWLQLDRRVLEHDFPKKSGPIVLYFCVRFYIESISYLKDNATIELFFLNAKSCIYKELIDVDSEVVFELAAYILQEAKGDFTSDDFTRADLKKLPALPTQALKEHPSLAYCEDRVIEYYKKLNGQTRGQAIVNYMSIVESLPTYGVHYYAVKDKQGIPWWLGLSYKGIFQYDHHDKVKPRKIFQWRQLENLYFREKKFSVEVHDPRRASVTRRTFGHGGIAVHTWYACAALIKSIWAMAISQHQFYLDRKQSKSKIHAARSLSEIAIDLTETGTLKTSKLANMGSKGKIISGSSGSLLSSGSQESDSSQSAKKDMLAALKSRQEALEETLRQRIEELKKLCVREAELTGKLPREYPLDPGEEPPTVRRRIGTSFKLDEQKLLAKGEEVELERLEREFAIQSQITEAARRLASDPHVSRKLKKQRKTSYLNALKKLQEIENSINEYRIKSGKKPTQRASLIIDEANLGSEDSSLSDALVLEDEDSQVPSILSPMQSPHKGLPPRPPSHARPHPPQSLDGLRQLHYQRNDYDKSPIKPKMWSESSLDEPYEKLKKRSSHSHSSHHKRFPSTGSCSEAGGSSSLQNSPIRTLPHWNSQSSMPSTPDLRVRSPHYVHSTRSVDISPTRLHSLAQHFRHRSSSLESQGKLLGSENDTGSPDFYTPRTRSSNGSDPMDDCSSCTSHSSSEHYYPTQMNPNYSTLAEDSPSKARERQRHKYKSNGNLVSSNSGSMPNLAARNGTGHHGVYLHSQSQPSSQYRIKEYPLYIEGSSSPVVVRSLENDQEGHYSVKAQFKTSNSYTAGGMYKENWHGDDSVDSVRLTPSRSQIVRTPSLGREGSEKGPGRTVVSNELRQWYHRSTSSHKEHNRLSHTSSNSSDSGSQYSTSQSTFVAHSRVTRMPPVCKATTAALPHGQRSSTPCSDLTATPPSSPHHILSWQTGSYNDSFYLDSALYQELADVQWYGQEKAKPGTLV

Target ORF information:

RefSeq Version	XM_012960029.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis FERM domain containing 4A (frmd4a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012960029.2

ORF Insert Sequence:

ATGACGGAGG GGCGGCGATG TCAAGTTCAC CTCCTAGATG ACAGGAAGCT GGAACTTCTT 
GTTCAGCCAA AGCTATTGGC CAAGGAGTTG CTTGATCTTG TTGCATCACA CTTCAATCTG 
AAAGAAAAGG AATATTTTGG GATAGCCTTT ACAGATGAAA CGGGCCATTT GAATTGGTTG 
CAGTTGGACC GCAGAGTACT AGAACACGAC TTTCCCAAAA AGTCTGGTCC GATTGTGCTG 
TACTTTTGTG TAAGGTTTTA CATAGAAAGT ATATCATACC TGAAGGACAA TGCGACCATT 
GAACTGTTCT TCCTTAATGC CAAGTCATGT ATATACAAGG AGTTAATAGA TGTTGACAGT 
GAAGTTGTTT TTGAATTAGC TGCGTATATC CTGCAGGAAG CAAAGGGAGA TTTTACAAGT 
GATGATTTTA CAAGAGCAGA CTTAAAGAAG TTACCAGCTC TTCCAACTCA GGCCCTGAAG 
GAGCACCCTT CACTTGCATA CTGTGAGGAC AGAGTAATAG AATACTACAA GAAACTGAAT 
GGCCAAACAA GAGGACAAGC TATTGTGAAT TATATGAGTA TTGTGGAGTC GCTTCCCACA 
TATGGAGTTC ATTATTATGC AGTCAAGGAC AAGCAAGGAA TCCCCTGGTG GTTGGGGTTA 
AGTTACAAAG GTATTTTCCA ATATGACCAT CATGATAAGG TGAAACCACG AAAGATCTTT 
CAATGGCGGC AGCTGGAAAA CCTTTACTTC AGGGAGAAGA AGTTTTCGGT AGAGGTTCAT 
GACCCTCGCA GGGCCTCTGT TACCAGAAGG ACATTTGGAC ATGGTGGAAT TGCAGTGCAC 
ACATGGTACG CATGCGCAGC CCTTATAAAG TCCATCTGGG CTATGGCCAT TAGCCAACAT 
CAATTCTATC TTGATAGGAA GCAAAGCAAG TCAAAAATAC ATGCTGCAAG GAGCCTGAGT 
GAGATTGCTA TTGACCTGAC AGAGACAGGA ACCCTAAAGA CTTCAAAACT TGCCAATATG 
GGCAGCAAAG GAAAAATAAT CAGCGGAAGC AGTGGGAGTC TGTTATCCTC TGGTTCCCAA 
GAGTCCGATA GTTCGCAGTC TGCCAAGAAA GACATGCTGG CTGCACTGAA ATCCAGGCAG 
GAAGCTCTGG AGGAGACACT ACGCCAGCGC ATAGAAGAGC TGAAGAAACT GTGTGTGCGG 
GAAGCGGAGC TCACAGGTAA ATTGCCAAGA GAGTATCCTT TAGATCCTGG GGAAGAGCCC 
CCTACAGTAC GAAGACGAAT AGGGACATCA TTTAAGCTTG ATGAGCAGAA ACTTCTAGCA 
AAAGGAGAGG AGGTCGAGCT GGAACGTCTG GAGAGGGAAT TTGCAATCCA GTCACAAATC 
ACAGAGGCGG CTCGTCGCTT GGCCAGTGAT CCACACGTCA GCAGGAAGCT GAAAAAACAA 
CGAAAGACCT CATATCTTAA CGCACTAAAA AAACTGCAGG AGATTGAAAA CTCAATAAAT 
GAGTACCGCA TCAAATCTGG GAAAAAGCCC ACGCAGAGAG CTTCCCTGAT TATAGATGAA 
GCCAATCTCG GCAGTGAAGA CAGCTCCCTC TCTGATGCTC TTGTGCTTGA AGATGAAGAC 
TCTCAGGTTC CCAGCATACT GTCTCCTATG CAGTCACCCC ACAAAGGTTT ACCACCAAGG 
CCCCCATCGC ATGCCAGACC CCACCCTCCA CAGTCACTGG ATGGACTGAG ACAGTTGCAT 
TACCAGAGAA ATGATTATGA CAAGTCACCC ATAAAGCCCA AAATGTGGAG TGAATCCTCC 
TTGGATGAAC CATATGAGAA ATTAAAAAAA AGATCATCAC ACAGTCATTC CAGTCACCAC 
AAAAGGTTTC CTAGCACAGG AAGTTGTTCT GAGGCCGGGG GAAGCAGTTC CCTACAGAAT 
AGCCCAATAC GAACACTGCC TCACTGGAAT TCCCAGTCCA GCATGCCCTC CACACCAGAC 
CTGCGAGTAC GCAGCCCACA CTATGTACAT TCAACCAGGT CAGTGGACAT CAGCCCCACC 
AGGCTGCACA GCCTAGCACA GCACTTTAGA CACCGGAGCT CAAGTTTGGA GTCACAGGGA 
AAGCTTTTGG GCTCTGAGAA TGACACTGGG AGCCCAGATT TCTACACCCC AAGGACTCGT 
AGTAGTAATG GCTCTGATCC CATGGATGAC TGTTCCTCCT GTACAAGCCA TTCCAGCTCA 
GAACACTACT ACCCAACTCA GATGAACCCT AATTACTCAA CATTGGCTGA AGATTCCCCC 
TCCAAGGCCA GGGAACGTCA GCGACATAAA TATAAATCTA ATGGCAACCT TGTATCCTCC 
AATTCAGGGA GTATGCCCAA CTTAGCTGCC AGAAATGGTA CAGGGCATCA CGGAGTCTAC 
CTTCATAGCC AGAGCCAGCC TTCATCTCAG TACAGGATCA AAGAGTATCC ACTTTACATT 
GAAGGAAGCT CATCGCCTGT GGTTGTGCGC AGTCTAGAGA ATGACCAAGA GGGACACTAC 
AGTGTCAAAG CACAATTCAA GACCTCTAAC TCTTACACTG CAGGAGGGAT GTACAAAGAG 
AACTGGCATG GTGATGATTC AGTAGATTCT GTGAGGCTTA CTCCCTCCCG CTCTCAGATT 
GTAAGAACTC CATCACTTGG GAGAGAGGGC TCTGAGAAGG GCCCAGGCAG GACTGTGGTT 
TCTAATGAAC TGCGCCAGTG GTACCACAGG TCTACGTCAT CTCACAAAGA GCACAACAGA 
CTTTCACACA CTAGTTCGAA TTCCTCTGAC AGTGGCTCCC AGTACAGCAC ATCTCAAAGC 
ACCTTTGTGG CACACAGTAG GGTAACAAGA ATGCCTCCAG TGTGTAAAGC AACAACAGCT 
GCCTTACCTC ATGGTCAGAG GAGTTCAACG CCTTGTAGTG ATCTCACAGC CACGCCTCCA 
AGTAGCCCTC ACCACATTCT ATCATGGCAG ACTGGGAGCT ACAATGATAG CTTCTACCTA 
GACTCTGCTC TGTATCAAGA ATTAGCTGAT GTCCAGTGGT ACGGACAAGA GAAAGCCAAG 
CCTGGGACTC TTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	frmd4a
Entrez Gene ID	733886
Full Name	FERM domain containing 4A
General protein information	Preferred Names FERM domain containing 4A Names FERM domain-containing protein 4A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	FRMD4A	NP_060497.3
Canis lupus familiaris (dog)	FRMD4A	XP_005617176.1
Rattus norvegicus (Norway rat)	Frmd4a	NP_001178750.1
Danio rerio (zebrafish)	si:ch211-220f12.1	NP_001122007.1
Xenopus tropicalis (tropical clawed frog)	frmd4a	XP_002935289.2
Mus musculus (house mouse)	Frmd4a	NP_766063.3
Gallus gallus (chicken)	LOC419035	XP_003640382.2
Pan troglodytes (chimpanzee)	FRMD4A	XP_001143334.2
Bos taurus (cattle)	FRMD4A	NP_001179196.1

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frmd4a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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