sema3e cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema3e
Entrez Gene ID	100494277
Full Name	semaphorin 3E
General protein information	Preferred Names semaphorin 3E Names semaphorin-3E sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935256.4	XP_002935302.1	semaphorin-3E
XM_002935256.5	XP_002935302.1	semaphorin-3E

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEMA3E	NP_036563.1
Pan troglodytes (chimpanzee)	SEMA3E	XP_527803.3
Mus musculus (house mouse)	Sema3e	NP_035478.2
Rattus norvegicus (Norway rat)	Sema3e	NP_001100049.1
	SEMA3E	XP_002803437.1
Gallus gallus (chicken)	SEMA3E	NP_989573.1
Danio rerio (zebrafish)	sema3e	NP_001038549.1
Canis lupus familiaris (dog)	SEMA3E	XP_005631079.1
Bos taurus (cattle)	SEMA3E	XP_003586003.2
Xenopus tropicalis (tropical clawed frog)	sema3e	XP_002935302.1

Gene Ontology

The following sema3e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema3e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa07912	XM_002935256.4 Latest version!	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (sema3e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa54875	XM_002935256.5 Latest version!	Xenopus tropicalis semaphorin 3E (sema3e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema3e ( XM_002935256.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935302.1 Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (sema3e), mRNA.

Price & Availability↑

CloneID	OXa07912
Clone ID Related Accession (Same CDS sequence)	XM_002935256.4
Accession Version	XM_002935256.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935256.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAG AACTGTCCTG TCCCAGTATG GCTTGTACCT TATGGAATGC ATTGTTTCTC 
CTTGGGCTTT TTTGGACATC ATTTGCTGTG AGTGATACCA CTCAGCCAAG GTTACGCCTG 
TCCCATAAAG AGCTCAGAGA TTTAAACAGA ACATCAGTTT TCCACAGCCC GTTTCTTGAC 
TTAAATATTA TGTATTTGGA TGAATATCAA GAAAGACTTT TTGTTGGAGG CAGAAATCTT 
CTGTATTCTT TAAGCCTGGA ACAAGTCAGT GAAAACTACA AAGAGATACA CTGGCCAAGT 
ACAGACCAGC AAGTTGAGGA CTGCAGTAGA AAGGGAAGAG ATAAGAGTGA ATGCGCCAAT 
TTTATACGAG TTCTTCATCA CTATAATAGA ACCCATCTTT TGGCATGTGG GACCGGTGCA 
TTTGACCCTG TATGTGCATT TATAAGAGCT GGACTCCGTT CACAGGAACA CCTTTTTCAG 
CTGGAACCCC AAAAATTTGA AAGGGGACGT GGAAGATGCC CATTTGACCC CAACTCTTCA 
GTAATTTCAA CATTAACTGG TGGGGAGTTA TTTGTAGGTC TCTACAATGA TTTCTGGGGA 
AGAGATGCAA CAATATTTCG GACCACGGGG CATTTGCCGC CCATTCGAAC AGAACATGAT 
GATGACCGAA TCCTCACAGA ACCTAAATTT GTAGGTTCCT ATATGATTCC AGATAATGAA 
GACAAGGATG ACAATAAAAT GTACTTTTTC TATACTGAAA AAGCAATAGA AGGAGACAAA 
GGAGTACAAG CAATTTATTC CAGAGTCGGG CGTGTCTGTG CAAACGACAT AGGAGGTCAG 
AGGATGATGG TAAATAAGTG GACCACTTTC CTAAAAACTA GACTAATCTG CTCAGTACCA 
GGACCAAATG GGATAGATAC ATTTTTTGAT GAACTTGAAG ATGTATTCTT GCTGCATACC 
AGGGATAACA AAAACCCAGA GATATTTGCA TTATTCAGTA CTACCAGTAA CATTTTCAAA 
GGATATGCCA TTTGTGTTTA TCATATGGCT AGTATCCGAG AGGCATTCAA TGGTCCATTT 
GCACACAAAG AAGGACCTGA GTACCACTGG TCAATATATG AAGGAAAAAT ACCTTACCCA 
AGGCCTGGAT TGTGTGCCAG CAAAGTGAAT GGAGGGCACT ATGGTACAAC TAAGGATTAT 
CCAGATGAAG CTCTGCGCTT TGCTAGAAGC CACCCATTGA TGTATGAGTC AGTTAAGCCA 
ACTCACAAAA AGCCAATTCT CATAAAAGCT GATGGAAAAT ACAACTTGAA ACAAATAGCT 
GTAGATAGGG TGGAGGCTGA GGATGGTCAA TACCACGTTG TGTTTATAGG AACAGATAAC 
GGAATAGTTC TGAAAGTCAT CACAATTTAT AATCAAGAGA CTGAAACAAT GGAAGAAGTA 
ATTCTTGAAG AACTTCAAGT ATTTAAGGTT CCTGATCCTA TAATTTCAAT GGAAATTTCA 
GCAAAAAGAC AACAACTTTA CATTGGTTCA GCATCCAGCA TTGTGCAAAT AAAGACTCAT 
CAGTGTGAGA TGTATGGGAC TGCATGTGCG GACTGCTGTC TGGCTAGAGA TCCATATTGT 
GCTTGGGATG GGATATCTTG TTCTCGATAC TATCCACTGG GATCACACTC CAAAAGACGG 
TTTAGAAGAC AAGATGTTCG TCATGGAAAC GCAGTTCAGC AGTGCTTTGG ACAACAGTTT 
CATGGAGAAA CACTAGAAAA GACTGAAGAA CACCTGGCAT ATGGAATAGA AAACAACAGT 
ACTCTTTTAG AATGTATGCC TCGCTCTTTG CAATCCAAAG TTGTCTGGTT TGTGCAAAGA 
GGACATGACA CAAGGAAGGA GGAGGTAAAA ACAGATGAAA GAGTTGTCAA AACAGATTTT 
GGACTTTTGT TCTTCAAGCT GCACAAAATG GATGCTGGAA TATATTTTTG TCAAACAGTT 
GAGCACATGT TTGTTCACAT AATCAGAAAA ATTACTCTTG AGATTGTGGA TGAAGAGAGG 
GTAGATGATA TGTTTCACAA AGAAGAAGAA GAAGAACCAG TTGCAAAAAT GCCTTGTCCT 
GTGCAGGCGC ATGTTCCACA AGCAACAAGG CCTTGGTATA AGGAGTTTCT ACAACTCATT 
GGTTATAGCA ACTTCCATAA AGTTGAGGAG TACTGTGAAA AAGTGTGGTG CACAGACAGG 
AGAAGGAAAA AGCTGAAAGC GCCACAAAAT AAATGGAAAT ATGGAAATCC ACAGGAACGA 
AAACCAAAAG TGAAATCAGA ACATTATCGG ATACCAAGGC ACCTTACTGG ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935302.1
CDS	540..2876
Translation	MKRELSCPSMACTLWNALFLLGLFWTSFAVSDTTQPRLRLSHKELRDLNRTSVFHSPFLDLNIMYLDEYQERLFVGGRNLLYSLSLEQVSENYKEIHWPSTDQQVEDCSRKGRDKSECANFIRVLHHYNRTHLLACGTGAFDPVCAFIRAGLRSQEHLFQLEPQKFERGRGRCPFDPNSSVISTLTGGELFVGLYNDFWGRDATIFRTTGHLPPIRTEHDDDRILTEPKFVGSYMIPDNEDKDDNKMYFFYTEKAIEGDKGVQAIYSRVGRVCANDIGGQRMMVNKWTTFLKTRLICSVPGPNGIDTFFDELEDVFLLHTRDNKNPEIFALFSTTSNIFKGYAICVYHMASIREAFNGPFAHKEGPEYHWSIYEGKIPYPRPGLCASKVNGGHYGTTKDYPDEALRFARSHPLMYESVKPTHKKPILIKADGKYNLKQIAVDRVEAEDGQYHVVFIGTDNGIVLKVITIYNQETETMEEVILEELQVFKVPDPIISMEISAKRQQLYIGSASSIVQIKTHQCEMYGTACADCCLARDPYCAWDGISCSRYYPLGSHSKRRFRRQDVRHGNAVQQCFGQQFHGETLEKTEEHLAYGIENNSTLLECMPRSLQSKVVWFVQRGHDTRKEEVKTDERVVKTDFGLLFFKLHKMDAGIYFCQTVEHMFVHIIRKITLEIVDEERVDDMFHKEEEEEPVAKMPCPVQAHVPQATRPWYKEFLQLIGYSNFHKVEEYCEKVWCTDRRRKKLKAPQNKWKYGNPQERKPKVKSEHYRIPRHLTGL

Target ORF information:

RefSeq Version	XM_002935256.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (sema3e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935256.4

ORF Insert Sequence:

ATGAAGAGAG AACTGTCCTG TCCCAGTATG GCTTGTACCT TATGGAATGC ATTGTTTCTC 
CTTGGGCTTT TTTGGACATC ATTTGCTGTG AGTGATACCA CTCAGCCAAG GTTACGCCTG 
TCCCATAAAG AGCTCAGAGA TTTAAACAGA ACATCAGTTT TCCACAGCCC GTTTCTTGAC 
TTAAATATTA TGTATTTGGA TGAATATCAA GAAAGACTTT TTGTTGGAGG CAGAAATCTT 
CTGTATTCTT TAAGCCTGGA ACAAGTCAGT GAAAACTACA AAGAGATACA CTGGCCAAGT 
ACAGACCAGC AAGTTGAGGA CTGCAGTAGA AAGGGAAGAG ATAAGAGTGA ATGCGCCAAT 
TTTATACGAG TTCTTCATCA CTATAATAGA ACCCATCTTT TGGCATGTGG GACCGGTGCA 
TTTGACCCTG TATGTGCATT TATAAGAGCT GGACTCCGTT CACAGGAACA CCTTTTTCAG 
CTGGAACCCC AAAAATTTGA AAGGGGACGT GGAAGATGCC CATTTGACCC CAACTCTTCA 
GTAATTTCAA CATTAACTGG TGGGGAGTTA TTTGTAGGTC TCTACAATGA TTTCTGGGGA 
AGAGATGCAA CAATATTTCG GACCACGGGG CATTTGCCGC CCATTCGAAC AGAACATGAT 
GATGACCGAA TCCTCACAGA ACCTAAATTT GTAGGTTCCT ATATGATTCC AGATAATGAA 
GACAAGGATG ACAATAAAAT GTACTTTTTC TATACTGAAA AAGCAATAGA AGGAGACAAA 
GGAGTACAAG CAATTTATTC CAGAGTCGGG CGTGTCTGTG CAAACGACAT AGGAGGTCAG 
AGGATGATGG TAAATAAGTG GACCACTTTC CTAAAAACTA GACTAATCTG CTCAGTACCA 
GGACCAAATG GGATAGATAC ATTTTTTGAT GAACTTGAAG ATGTATTCTT GCTGCATACC 
AGGGATAACA AAAACCCAGA GATATTTGCA TTATTCAGTA CTACCAGTAA CATTTTCAAA 
GGATATGCCA TTTGTGTTTA TCATATGGCT AGTATCCGAG AGGCATTCAA TGGTCCATTT 
GCACACAAAG AAGGACCTGA GTACCACTGG TCAATATATG AAGGAAAAAT ACCTTACCCA 
AGGCCTGGAT TGTGTGCCAG CAAAGTGAAT GGAGGGCACT ATGGTACAAC TAAGGATTAT 
CCAGATGAAG CTCTGCGCTT TGCTAGAAGC CACCCATTGA TGTATGAGTC AGTTAAGCCA 
ACTCACAAAA AGCCAATTCT CATAAAAGCT GATGGAAAAT ACAACTTGAA ACAAATAGCT 
GTAGATAGGG TGGAGGCTGA GGATGGTCAA TACCACGTTG TGTTTATAGG AACAGATAAC 
GGAATAGTTC TGAAAGTCAT CACAATTTAT AATCAAGAGA CTGAAACAAT GGAAGAAGTA 
ATTCTTGAAG AACTTCAAGT ATTTAAGGTT CCTGATCCTA TAATTTCAAT GGAAATTTCA 
GCAAAAAGAC AACAACTTTA CATTGGTTCA GCATCCAGCA TTGTGCAAAT AAAGACTCAT 
CAGTGTGAGA TGTATGGGAC TGCATGTGCG GACTGCTGTC TGGCTAGAGA TCCATATTGT 
GCTTGGGATG GGATATCTTG TTCTCGATAC TATCCACTGG GATCACACTC CAAAAGACGG 
TTTAGAAGAC AAGATGTTCG TCATGGAAAC GCAGTTCAGC AGTGCTTTGG ACAACAGTTT 
CATGGAGAAA CACTAGAAAA GACTGAAGAA CACCTGGCAT ATGGAATAGA AAACAACAGT 
ACTCTTTTAG AATGTATGCC TCGCTCTTTG CAATCCAAAG TTGTCTGGTT TGTGCAAAGA 
GGACATGACA CAAGGAAGGA GGAGGTAAAA ACAGATGAAA GAGTTGTCAA AACAGATTTT 
GGACTTTTGT TCTTCAAGCT GCACAAAATG GATGCTGGAA TATATTTTTG TCAAACAGTT 
GAGCACATGT TTGTTCACAT AATCAGAAAA ATTACTCTTG AGATTGTGGA TGAAGAGAGG 
GTAGATGATA TGTTTCACAA AGAAGAAGAA GAAGAACCAG TTGCAAAAAT GCCTTGTCCT 
GTGCAGGCGC ATGTTCCACA AGCAACAAGG CCTTGGTATA AGGAGTTTCT ACAACTCATT 
GGTTATAGCA ACTTCCATAA AGTTGAGGAG TACTGTGAAA AAGTGTGGTG CACAGACAGG 
AGAAGGAAAA AGCTGAAAGC GCCACAAAAT AAATGGAAAT ATGGAAATCC ACAGGAACGA 
AAACCAAAAG TGAAATCAGA ACATTATCGG ATACCAAGGC ACCTTACTGG ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema3e ( XM_002935256.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935302.1 Xenopus tropicalis semaphorin 3E (sema3e), mRNA.

Price & Availability↑

CloneID	OXa54875
Clone ID Related Accession (Same CDS sequence)	XM_002935256.5
Accession Version	XM_002935256.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-3E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935256.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAG AACTGTCCTG TCCCAGTATG GCTTGTACCT TATGGAATGC ATTGTTTCTC 
CTTGGGCTTT TTTGGACATC ATTTGCTGTG AGTGATACCA CTCAGCCAAG GTTACGCCTG 
TCCCATAAAG AGCTCAGAGA TTTAAACAGA ACATCAGTTT TCCACAGCCC GTTTCTTGAC 
TTAAATATTA TGTATTTGGA TGAATATCAA GAAAGACTTT TTGTTGGAGG CAGAAATCTT 
CTGTATTCTT TAAGCCTGGA ACAAGTCAGT GAAAACTACA AAGAGATACA CTGGCCAAGT 
ACAGACCAGC AAGTTGAGGA CTGCAGTAGA AAGGGAAGAG ATAAGAGTGA ATGCGCCAAT 
TTTATACGAG TTCTTCATCA CTATAATAGA ACCCATCTTT TGGCATGTGG GACCGGTGCA 
TTTGACCCTG TATGTGCATT TATAAGAGCT GGACTCCGTT CACAGGAACA CCTTTTTCAG 
CTGGAACCCC AAAAATTTGA AAGGGGACGT GGAAGATGCC CATTTGACCC CAACTCTTCA 
GTAATTTCAA CATTAACTGG TGGGGAGTTA TTTGTAGGTC TCTACAATGA TTTCTGGGGA 
AGAGATGCAA CAATATTTCG GACCACGGGG CATTTGCCGC CCATTCGAAC AGAACATGAT 
GATGACCGAA TCCTCACAGA ACCTAAATTT GTAGGTTCCT ATATGATTCC AGATAATGAA 
GACAAGGATG ACAATAAAAT GTACTTTTTC TATACTGAAA AAGCAATAGA AGGAGACAAA 
GGAGTACAAG CAATTTATTC CAGAGTCGGG CGTGTCTGTG CAAACGACAT AGGAGGTCAG 
AGGATGATGG TAAATAAGTG GACCACTTTC CTAAAAACTA GACTAATCTG CTCAGTACCA 
GGACCAAATG GGATAGATAC ATTTTTTGAT GAACTTGAAG ATGTATTCTT GCTGCATACC 
AGGGATAACA AAAACCCAGA GATATTTGCA TTATTCAGTA CTACCAGTAA CATTTTCAAA 
GGATATGCCA TTTGTGTTTA TCATATGGCT AGTATCCGAG AGGCATTCAA TGGTCCATTT 
GCACACAAAG AAGGACCTGA GTACCACTGG TCAATATATG AAGGAAAAAT ACCTTACCCA 
AGGCCTGGAT TGTGTGCCAG CAAAGTGAAT GGAGGGCACT ATGGTACAAC TAAGGATTAT 
CCAGATGAAG CTCTGCGCTT TGCTAGAAGC CACCCATTGA TGTATGAGTC AGTTAAGCCA 
ACTCACAAAA AGCCAATTCT CATAAAAGCT GATGGAAAAT ACAACTTGAA ACAAATAGCT 
GTAGATAGGG TGGAGGCTGA GGATGGTCAA TACCACGTTG TGTTTATAGG AACAGATAAC 
GGAATAGTTC TGAAAGTCAT CACAATTTAT AATCAAGAAA CTGAAACAAT GGAAGAAGTA 
ATTCTTGAAG AACTTCAAGT ATTTAAGGTT CCTGATCCTA TAATTTCAAT GGAAATTTCA 
GCAAAAAGAC AACAACTTTA CATTGGTTCA GCATCCAGCA TTGTGCAAAT AAAGACTCAT 
CAGTGTGAGA TGTATGGGAC TGCATGTGCG GACTGCTGTC TGGCTAGAGA TCCATATTGT 
GCTTGGGATG GGATATCTTG TTCTCGATAC TATCCACTGG GATCACACTC CAAAAGACGG 
TTTAGAAGAC AAGATGTTCG TCATGGAAAC GCAGTTCAGC AGTGCTTTGG ACAACAGTTT 
CATGGAGAAA CACTAGAAAA GACTGAAGAA CACCTGGCAT ATGGAATAGA AAACAACAGT 
ACTCTTTTAG AATGTATGCC TCGCTCTTTG CAATCCAAAG TTGTCTGGTT TGTGCAAAGA 
GGACATGACA CAAGGAAGGA GGAGGTAAAA ACAGATGAAA GAGTTGTCAA AACAGATTTT 
GGACTTTTGT TCTTCAAGCT GCACAAAATG GATGCTGGAA TATATTTTTG TCAAACAGTT 
GAGCACATGT TTGTTCACAT AATCAGAAAA ATTACTCTTG AGATTGTGGA TGAAGAGAGG 
GTAGATGATA TGTTTCACAA AGAAGAAGAA GAAGAACCAG TTGCAAAAAT GCCTTGTCCT 
GTGCAGGCGC ATGTTCCACA AGCAACAAGG CCTTGGTATA AGGAGTTTCT ACAACTCATT 
GGTTATAGCA ACTTCCATAA AGTTGAGGAG TACTGTGAAA AAGTGTGGTG CACAGACAGG 
AGAAGGAAAA AGCTGAAAGC GCCACAAAAT AAATGGAAAT ATGGAAATCC ACAGGAACGA 
AAACCAAAAG TGAAATCAGA ACATTATCGG ATACCAAGGC ACCTTACTGG ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935302.1
CDS	537..2873
Translation	MKRELSCPSMACTLWNALFLLGLFWTSFAVSDTTQPRLRLSHKELRDLNRTSVFHSPFLDLNIMYLDEYQERLFVGGRNLLYSLSLEQVSENYKEIHWPSTDQQVEDCSRKGRDKSECANFIRVLHHYNRTHLLACGTGAFDPVCAFIRAGLRSQEHLFQLEPQKFERGRGRCPFDPNSSVISTLTGGELFVGLYNDFWGRDATIFRTTGHLPPIRTEHDDDRILTEPKFVGSYMIPDNEDKDDNKMYFFYTEKAIEGDKGVQAIYSRVGRVCANDIGGQRMMVNKWTTFLKTRLICSVPGPNGIDTFFDELEDVFLLHTRDNKNPEIFALFSTTSNIFKGYAICVYHMASIREAFNGPFAHKEGPEYHWSIYEGKIPYPRPGLCASKVNGGHYGTTKDYPDEALRFARSHPLMYESVKPTHKKPILIKADGKYNLKQIAVDRVEAEDGQYHVVFIGTDNGIVLKVITIYNQETETMEEVILEELQVFKVPDPIISMEISAKRQQLYIGSASSIVQIKTHQCEMYGTACADCCLARDPYCAWDGISCSRYYPLGSHSKRRFRRQDVRHGNAVQQCFGQQFHGETLEKTEEHLAYGIENNSTLLECMPRSLQSKVVWFVQRGHDTRKEEVKTDERVVKTDFGLLFFKLHKMDAGIYFCQTVEHMFVHIIRKITLEIVDEERVDDMFHKEEEEEPVAKMPCPVQAHVPQATRPWYKEFLQLIGYSNFHKVEEYCEKVWCTDRRRKKLKAPQNKWKYGNPQERKPKVKSEHYRIPRHLTGL

Target ORF information:

RefSeq Version	XM_002935256.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 3E (sema3e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935256.5

ORF Insert Sequence:

ATGAAGAGAG AACTGTCCTG TCCCAGTATG GCTTGTACCT TATGGAATGC ATTGTTTCTC 
CTTGGGCTTT TTTGGACATC ATTTGCTGTG AGTGATACCA CTCAGCCAAG GTTACGCCTG 
TCCCATAAAG AGCTCAGAGA TTTAAACAGA ACATCAGTTT TCCACAGCCC GTTTCTTGAC 
TTAAATATTA TGTATTTGGA TGAATATCAA GAAAGACTTT TTGTTGGAGG CAGAAATCTT 
CTGTATTCTT TAAGCCTGGA ACAAGTCAGT GAAAACTACA AAGAGATACA CTGGCCAAGT 
ACAGACCAGC AAGTTGAGGA CTGCAGTAGA AAGGGAAGAG ATAAGAGTGA ATGCGCCAAT 
TTTATACGAG TTCTTCATCA CTATAATAGA ACCCATCTTT TGGCATGTGG GACCGGTGCA 
TTTGACCCTG TATGTGCATT TATAAGAGCT GGACTCCGTT CACAGGAACA CCTTTTTCAG 
CTGGAACCCC AAAAATTTGA AAGGGGACGT GGAAGATGCC CATTTGACCC CAACTCTTCA 
GTAATTTCAA CATTAACTGG TGGGGAGTTA TTTGTAGGTC TCTACAATGA TTTCTGGGGA 
AGAGATGCAA CAATATTTCG GACCACGGGG CATTTGCCGC CCATTCGAAC AGAACATGAT 
GATGACCGAA TCCTCACAGA ACCTAAATTT GTAGGTTCCT ATATGATTCC AGATAATGAA 
GACAAGGATG ACAATAAAAT GTACTTTTTC TATACTGAAA AAGCAATAGA AGGAGACAAA 
GGAGTACAAG CAATTTATTC CAGAGTCGGG CGTGTCTGTG CAAACGACAT AGGAGGTCAG 
AGGATGATGG TAAATAAGTG GACCACTTTC CTAAAAACTA GACTAATCTG CTCAGTACCA 
GGACCAAATG GGATAGATAC ATTTTTTGAT GAACTTGAAG ATGTATTCTT GCTGCATACC 
AGGGATAACA AAAACCCAGA GATATTTGCA TTATTCAGTA CTACCAGTAA CATTTTCAAA 
GGATATGCCA TTTGTGTTTA TCATATGGCT AGTATCCGAG AGGCATTCAA TGGTCCATTT 
GCACACAAAG AAGGACCTGA GTACCACTGG TCAATATATG AAGGAAAAAT ACCTTACCCA 
AGGCCTGGAT TGTGTGCCAG CAAAGTGAAT GGAGGGCACT ATGGTACAAC TAAGGATTAT 
CCAGATGAAG CTCTGCGCTT TGCTAGAAGC CACCCATTGA TGTATGAGTC AGTTAAGCCA 
ACTCACAAAA AGCCAATTCT CATAAAAGCT GATGGAAAAT ACAACTTGAA ACAAATAGCT 
GTAGATAGGG TGGAGGCTGA GGATGGTCAA TACCACGTTG TGTTTATAGG AACAGATAAC 
GGAATAGTTC TGAAAGTCAT CACAATTTAT AATCAAGAAA CTGAAACAAT GGAAGAAGTA 
ATTCTTGAAG AACTTCAAGT ATTTAAGGTT CCTGATCCTA TAATTTCAAT GGAAATTTCA 
GCAAAAAGAC AACAACTTTA CATTGGTTCA GCATCCAGCA TTGTGCAAAT AAAGACTCAT 
CAGTGTGAGA TGTATGGGAC TGCATGTGCG GACTGCTGTC TGGCTAGAGA TCCATATTGT 
GCTTGGGATG GGATATCTTG TTCTCGATAC TATCCACTGG GATCACACTC CAAAAGACGG 
TTTAGAAGAC AAGATGTTCG TCATGGAAAC GCAGTTCAGC AGTGCTTTGG ACAACAGTTT 
CATGGAGAAA CACTAGAAAA GACTGAAGAA CACCTGGCAT ATGGAATAGA AAACAACAGT 
ACTCTTTTAG AATGTATGCC TCGCTCTTTG CAATCCAAAG TTGTCTGGTT TGTGCAAAGA 
GGACATGACA CAAGGAAGGA GGAGGTAAAA ACAGATGAAA GAGTTGTCAA AACAGATTTT 
GGACTTTTGT TCTTCAAGCT GCACAAAATG GATGCTGGAA TATATTTTTG TCAAACAGTT 
GAGCACATGT TTGTTCACAT AATCAGAAAA ATTACTCTTG AGATTGTGGA TGAAGAGAGG 
GTAGATGATA TGTTTCACAA AGAAGAAGAA GAAGAACCAG TTGCAAAAAT GCCTTGTCCT 
GTGCAGGCGC ATGTTCCACA AGCAACAAGG CCTTGGTATA AGGAGTTTCT ACAACTCATT 
GGTTATAGCA ACTTCCATAA AGTTGAGGAG TACTGTGAAA AAGTGTGGTG CACAGACAGG 
AGAAGGAAAA AGCTGAAAGC GCCACAAAAT AAATGGAAAT ATGGAAATCC ACAGGAACGA 
AAACCAAAAG TGAAATCAGA ACATTATCGG ATACCAAGGC ACCTTACTGG ACTATAA