lrrc8a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrrc8a
Entrez Gene ID	100492279
Full Name	leucine rich repeat containing 8 VRAC subunit A
Synonyms	agm5,lrrc8,swell1
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit A Names volume-regulated anion channel subunit LRRC8A leucine-rich repeat containing 8 family member A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935506.4	XP_002935552.1	volume-regulated anion channel subunit LRRC8A

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8A	NP_001120717.1
Bos taurus (cattle)	LRRC8A	NP_001070275.1
Danio rerio (zebrafish)	lrrc8a	NP_001025120.1
Danio rerio (zebrafish)	si:ch211-236k19.7	XP_005165659.1
Xenopus tropicalis (tropical clawed frog)	lrrc8a	XP_004916747.1
	LRRC8A	XP_001109698.1
Mus musculus (house mouse)	Lrrc8a	NP_808393.1
Gallus gallus (chicken)	LOC100858917	XP_003642331.1
Gallus gallus (chicken)	LRRC8A	XP_415484.1
Pan troglodytes (chimpanzee)	LRRC8A	XP_520301.1
Canis lupus familiaris (dog)	LRRC8A	XP_005625321.1
Rattus norvegicus (Norway rat)	Lrrc8a	NP_001019953.1

Gene Ontology

The following lrrc8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrrc8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa14108	XM_002935506.4 Latest version!	Xenopus tropicalis leucine rich repeat containing 8 VRAC subunit A (lrrc8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrrc8a ( XM_002935506.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935552.1 Xenopus tropicalis leucine rich repeat containing 8 VRAC subunit A (lrrc8a), mRNA.

Price & Availability↑

CloneID	OXa14108
Clone ID Related Accession (Same CDS sequence)	XM_002935506.4
Accession Version	XM_002935506.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935506.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCGG TAACAGAGTT GCGGTACTTT GCTGACACCC AGCCGGCTTA CCGTATCCTC 
AAACCATGGT GGGACGTCTT CACGGACTAC ATCTCCATCG TCATGCTTAT GATAGCAGTG 
TTCGGGGGCA CCCTGCAGGT CACGCAGGAC AAGATGATCT GTCTTCCGTG CAAGTGGGTC 
ACTCATGACT CCTGCAATGA TTCCTACCGG GCCTGGAATG TGCCAGAGAC AGATCTCTAT 
ACAAATTCTA CCCTGTCACC TCCACTTGCA CCAGGACCCA CAGGTATTAA ATATGACCTG 
GATAGGCATC AGTACAACTA TGTGGATGCA GTGTGCTACG AGAACCGCTT GCACTGGTTT 
GCCAAGTACT TTCCCTACCT TGTCCTGCTG CACACGTTGA TCTTCCTGGC CTGCAGCAAC 
TTCTGGTTCA AGTTCCCCCG TACAAGCTCA AAACTTGAGC ATTTCGTCTC CATCTTGCTT 
AAATGCTTTG ATTCTCCCTG GACCACCCGG GCCCTCTCAG AGACAGTCGT TGAAGAGAGT 
GACCCAAAGC CCACCGGAGG AAAGATGAAT GGCTCAGTAG ACAAAAAGTC CTCCACTGCA 
AGCGAAGATG TGGAAGCAAC AGTGCCTATG CTCCAGAGGA GCAAGTCAAG GGTCGAGCAG 
GGGATTGTGG ACAGGTCAGA GACGGGTGTG CTGGACAAAA AGGAAGGAGA GCAAGCCAAA 
GCACTGTTTG AAAAGGTGAA AAAGTTCAGG ACTCACGTGG AGGAAGGGGA TATTGTGTAC 
CGGCTGTACA TGCGTCAGAC TATCATCAAA GTCATTAAGT TCATCATTAT TTTGTGCTAC 
ACTGTGTACT ACGTTAGCAG CATTAAATTC GACGTGGACT GCAAGGTGGA CATAGAGAGC 
CTGACTGGGT ACCGGATGTA CCGATGTGCC CACCCGCTGG CTACTCTCTT CAAGATCCTG 
GCATCTTTCT ACATCAGCCT GGTGGGTTTC TACGGCCTAG TCTGTGTTTA CACTCTCTGG 
TGGATGCTGA GACGTTCTTT AAAGAAGTAT TCCTTTGAGT CCATCAGGGA GGAGAGCAGC 
TACAGCGACA TCCCCGATGT CAAGAACGAC TTTGCTTTCA TGCTGCACCT CATAGACCAG 
TACGATCCTC TCTACTCCAA GCGTTTTGCT GTGTTCTTGT CGGAGGTCAG CGAGAACAAA 
CTTCGCCAGC TTAATCTCAA TCACGAGTGG ACCCTCGACA AACTGCGGCA GAGGATCACA 
AAGAATTCTC AGGAGAAACT GGAGCTGCAT CTTTTCATGC TCAGCGGGAT ACCTGACACG 
GTCTTCGACT TGGTAGAGCT GGAAGTGCTA AAACTCGAGT TAATACCAGA TGTCACTATC 
CCTCCAAGTA TTGCCCAGCT CACTGGCCTG AAAGAGCTAT GGCTGTACCA CACAGCAGCT 
AAAATTGAAG CTCCGGCTCT TGCCTTCCTG CGGGAGAACC TTAAGTCACT ACACATCAAG 
TTCACGGACA TCAAGGAGAT CCCCTTGTGG ATATACAGCC TTAAGAACCT GGAGGAACTC 
CACCTGACTG GCAACTTGAG CGCAGAAAAC AACCGGTACA TTGTTATCGA TGGCCTCCGG 
GAGCTGAAGA GGCTCAAAGT TCTGAGATTG AAGAGCAACC TTACTAAGCT GCCTCAGGTG 
GTGACAGACG TCGGGGTCCA CTTGCAGAAG CTGTCCGTCA ACAATGAGGG AACAAAGCTG 
ATGGTTCTCA ACAGTCTTAA AAAGATGGTC AACCTGACTG AACTGGAGCT GGTGCGCTGT 
GACTTAGAGA GAATTCCTCA CTCTATCTTC AGCCTTCACA ACCTGCAAGA GATTGACTTG 
AAGGACAACA ATTTAAAGAC CATTGAAGAG ATCATAAGCT TCCAGCACCT GCACCGGCTC 
ACATGCCTGA AGTTATGGTA CAATCACATA GCCTATATAC CCATTCAGAT CGGCAATCTG 
GCTAGCCTTG AACGGCTCTA CCTGAACCGC AACAAGATAG AGAAAATCCC CACCCAGCTC 
TTCCTGTGCC GCAAGCTTCG GCATCTAGAC CTGAGCCACA ATTCCCTCAG CAGCATCCCG 
CCAGAAATTG GCCACCTGCA GAGCCTGCAG TACCTTGCTG TAACCGCCAA CCATATTGAA 
AACCTGCCGG CCGAGCTGTT CCTGTGCAAG AAGCTCCGTA CGCTCAACTT GGGTAACAAC 
GTTTTGCAGT CTCTGCCTTC CCGAGTGGGT GAGCTCACCA ACCTATCCCA GGTGGAGCTT 
CGGGGTAACC GCTTAGAGTA CCTGCCGGTG GAGCTGGGTG ACTGTCACCT TCTGAAGCGC 
AGCGGCCTTG TAGTAGAAGA AGACTTGTTC AACACTCTTC CCCTGGACGT TAAAGAGAAG 
CTTAGCCGGT CAGAAAAGGA GCAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935552.1
CDS	201..2630
Translation	MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTHDSCNDSYRAWNVPETDLYTNSTLSPPLAPGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPTGGKMNGSVDKKSSTASEDVEATVPMLQRSKSRVEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFIIILCYTVYYVSSIKFDVDCKVDIESLTGYRMYRCAHPLATLFKILASFYISLVGFYGLVCVYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNHEWTLDKLRQRITKNSQEKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLKSLHIKFTDIKEIPLWIYSLKNLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLTKLPQVVTDVGVHLQKLSVNNEGTKLMVLNSLKKMVNLTELELVRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLASLERLYLNRNKIEKIPTQLFLCRKLRHLDLSHNSLSSIPPEIGHLQSLQYLAVTANHIENLPAELFLCKKLRTLNLGNNVLQSLPSRVGELTNLSQVELRGNRLEYLPVELGDCHLLKRSGLVVEEDLFNTLPLDVKEKLSRSEKEQV

Target ORF information:

RefSeq Version	XM_002935506.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis leucine rich repeat containing 8 VRAC subunit A (lrrc8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935506.4

ORF Insert Sequence:

ATGATTCCGG TAACAGAGTT GCGGTACTTT GCTGACACCC AGCCGGCTTA CCGTATCCTC 
AAACCATGGT GGGACGTCTT CACGGACTAC ATCTCCATCG TCATGCTTAT GATAGCAGTG 
TTCGGGGGCA CCCTGCAGGT CACGCAGGAC AAGATGATCT GTCTTCCGTG CAAGTGGGTC 
ACTCATGACT CCTGCAATGA TTCCTACCGG GCCTGGAATG TGCCAGAGAC AGATCTCTAT 
ACAAATTCTA CCCTGTCACC TCCACTTGCA CCAGGACCCA CAGGTATTAA ATATGACCTG 
GATAGGCATC AGTACAACTA TGTGGATGCA GTGTGCTACG AGAACCGCTT GCACTGGTTT 
GCCAAGTACT TTCCCTACCT TGTCCTGCTG CACACGTTGA TCTTCCTGGC CTGCAGCAAC 
TTCTGGTTCA AGTTCCCCCG TACAAGCTCA AAACTTGAGC ATTTCGTCTC CATCTTGCTT 
AAATGCTTTG ATTCTCCCTG GACCACCCGG GCCCTCTCAG AGACAGTCGT TGAAGAGAGT 
GACCCAAAGC CCACCGGAGG AAAGATGAAT GGCTCAGTAG ACAAAAAGTC CTCCACTGCA 
AGCGAAGATG TGGAAGCAAC AGTGCCTATG CTCCAGAGGA GCAAGTCAAG GGTCGAGCAG 
GGGATTGTGG ACAGGTCAGA GACGGGTGTG CTGGACAAAA AGGAAGGAGA GCAAGCCAAA 
GCACTGTTTG AAAAGGTGAA AAAGTTCAGG ACTCACGTGG AGGAAGGGGA TATTGTGTAC 
CGGCTGTACA TGCGTCAGAC TATCATCAAA GTCATTAAGT TCATCATTAT TTTGTGCTAC 
ACTGTGTACT ACGTTAGCAG CATTAAATTC GACGTGGACT GCAAGGTGGA CATAGAGAGC 
CTGACTGGGT ACCGGATGTA CCGATGTGCC CACCCGCTGG CTACTCTCTT CAAGATCCTG 
GCATCTTTCT ACATCAGCCT GGTGGGTTTC TACGGCCTAG TCTGTGTTTA CACTCTCTGG 
TGGATGCTGA GACGTTCTTT AAAGAAGTAT TCCTTTGAGT CCATCAGGGA GGAGAGCAGC 
TACAGCGACA TCCCCGATGT CAAGAACGAC TTTGCTTTCA TGCTGCACCT CATAGACCAG 
TACGATCCTC TCTACTCCAA GCGTTTTGCT GTGTTCTTGT CGGAGGTCAG CGAGAACAAA 
CTTCGCCAGC TTAATCTCAA TCACGAGTGG ACCCTCGACA AACTGCGGCA GAGGATCACA 
AAGAATTCTC AGGAGAAACT GGAGCTGCAT CTTTTCATGC TCAGCGGGAT ACCTGACACG 
GTCTTCGACT TGGTAGAGCT GGAAGTGCTA AAACTCGAGT TAATACCAGA TGTCACTATC 
CCTCCAAGTA TTGCCCAGCT CACTGGCCTG AAAGAGCTAT GGCTGTACCA CACAGCAGCT 
AAAATTGAAG CTCCGGCTCT TGCCTTCCTG CGGGAGAACC TTAAGTCACT ACACATCAAG 
TTCACGGACA TCAAGGAGAT CCCCTTGTGG ATATACAGCC TTAAGAACCT GGAGGAACTC 
CACCTGACTG GCAACTTGAG CGCAGAAAAC AACCGGTACA TTGTTATCGA TGGCCTCCGG 
GAGCTGAAGA GGCTCAAAGT TCTGAGATTG AAGAGCAACC TTACTAAGCT GCCTCAGGTG 
GTGACAGACG TCGGGGTCCA CTTGCAGAAG CTGTCCGTCA ACAATGAGGG AACAAAGCTG 
ATGGTTCTCA ACAGTCTTAA AAAGATGGTC AACCTGACTG AACTGGAGCT GGTGCGCTGT 
GACTTAGAGA GAATTCCTCA CTCTATCTTC AGCCTTCACA ACCTGCAAGA GATTGACTTG 
AAGGACAACA ATTTAAAGAC CATTGAAGAG ATCATAAGCT TCCAGCACCT GCACCGGCTC 
ACATGCCTGA AGTTATGGTA CAATCACATA GCCTATATAC CCATTCAGAT CGGCAATCTG 
GCTAGCCTTG AACGGCTCTA CCTGAACCGC AACAAGATAG AGAAAATCCC CACCCAGCTC 
TTCCTGTGCC GCAAGCTTCG GCATCTAGAC CTGAGCCACA ATTCCCTCAG CAGCATCCCG 
CCAGAAATTG GCCACCTGCA GAGCCTGCAG TACCTTGCTG TAACCGCCAA CCATATTGAA 
AACCTGCCGG CCGAGCTGTT CCTGTGCAAG AAGCTCCGTA CGCTCAACTT GGGTAACAAC 
GTTTTGCAGT CTCTGCCTTC CCGAGTGGGT GAGCTCACCA ACCTATCCCA GGTGGAGCTT 
CGGGGTAACC GCTTAGAGTA CCTGCCGGTG GAGCTGGGTG ACTGTCACCT TCTGAAGCGC 
AGCGGCCTTG TAGTAGAAGA AGACTTGTTC AACACTCTTC CCCTGGACGT TAAAGAGAAG 
CTTAGCCGGT CAGAAAAGGA GCAGGTGTGA