tmem132e cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmem132e
Entrez Gene ID	100491853
Full Name	transmembrane protein 132E
General protein information	Preferred Names transmembrane protein 132E Names transmembrane protein 132E
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940528.4	XP_002940574.2	transmembrane protein 132E
XM_002940528.5	XP_002940574.2	transmembrane protein 132E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tmem132e	NP_001127882.1
Drosophila melanogaster (fruit fly)	CG14446	NP_001245547.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004670	XP_318358.5
Pan troglodytes (chimpanzee)	TMEM132E	XP_523601.3
Bos taurus (cattle)	TMEM132E	XP_002695701.2
Gallus gallus (chicken)	TMEM132E	XP_001234668.3
Danio rerio (zebrafish)	tmem132e	XP_692496.5
Mus musculus (house mouse)	Tmem132e	NP_075927.2
Homo sapiens (human)	TMEM132E	NP_997196.1
	TMEM132E	XP_001113490.1
Canis lupus familiaris (dog)	TMEM132E	XP_548267.3
Xenopus tropicalis (tropical clawed frog)	LOC100491853	XP_002940574.2

Gene Ontology

The following tmem132e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmem132e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05637	XM_002940528.5 Latest version!	Xenopus tropicalis transmembrane protein 132E (tmem132e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
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OXa05637	XM_002940528.4	Xenopus tropicalis transmembrane protein 132E (tmem132e), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmem132e ( XM_002940528.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940574.2 Xenopus tropicalis transmembrane protein 132E (tmem132e), mRNA.

Price & Availability↑

CloneID	OXa05637
Clone ID Related Accession (Same CDS sequence)	XM_002940528.4 , XM_002940528.5
Accession Version	XM_002940528.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transmembrane protein 132E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940528.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAA GGAGGAGGTA TCCAGGACTT TTATGCAGCT ATTACTACTA CAATGGGTAT 
TCTAACTACC ACTACATGTA CTGCTGCCTT CTCTTTCTTA TTGCTACAAG GGCAACCTGC 
AAAGTACTGC CTACAAACTC AGCCCATCCC ACAGCACTTC CACCATCCCC AGCTGCCATA 
CTTCCAGTCA GCTATCGCCT GTCTAATACA CGTTTGGCCT TCTTCTTGAG AGAGGCTGGA 
CCTCTTCCAG CATGGAATTC AAGCACCCCA TTGTTACAAC GTTCAGAACC TTTTGTGGTG 
TTTCAAACAA AAGAGTTACC AATCCTTAAT GTTACTTTGG GACCTTTTGC TGCTGGGCAG 
GTACTACCAA AAGAGCTGCT GCAACCTTCA AGCACCCTAG AGGTCCCTGA CCAATTAACC 
GTAAACTGGA AAATCCGTGC CTACATAATT CGCACAAAGA TACCTTCTAC TCATCCAGTT 
GTACAAGTTC TTTTTTATGT AGCTGGCCGG GACTGGGATG ACTTTAGCGT TACAGAACGT 
CTACCCTGTG TTCGACTTCA CATTTTCCGG GATGTAAGAG ACATAAAAAG TTCTTGCCGA 
CTTCGAGGAA ACCTAGCTAC TTGCCTTGCA CAGGTTGAGC TACCACAGAA CTGGTTTGGA 
TTGGCAGTTG CACCCATGGG ACGTAGAAAA TCTTCATCTG ACGGGGCAGA ACTTAATGAA 
GAAAGCCTAC AAATAGAATT GTTTTATACT TTGCATGCTC CGGACTCGGC TGGTGGGTGT 
TCAAATGAAC CAGGAACACG TAGGGGAGGA GAAGCCCCAA CTCAACATCC CCTTCTTCGT 
ATTGGAAGTA TCAGTCTATA CAAGCCTATT CTAGTTGAGC AGTTGCAAGA ACAACAGTTG 
GATACTAATG TCTTTATCAG ACTTCCAGAC CGCCCACTAA GGCCAGGAGA GATCCTAAGC 
ATAATGCTAT ATCTGGTAGC CAATTCCACC GTAGAACACT TCACACTCAG GGTGAAGGCA 
AAGAAGGGAA TAAACCTGCA GAGTACCAAA TCAAGGGATC CCCAATGGCT GGTCAACTCT 
GAGTTGCTGA CTGGTGGAAA ACATTCCACA GCGACTGTAG ATGTGTCCCG AGTTCAAGGA 
ATGCCCTATG AGAGTGAAGA TACCCCTTAC CACATAATGC AGCTTGACTT TGAAATGGAG 
AATTTCACCA GTCAGTCAGT CACAAGACGA ATAATGTGGC ATGTAGATTA CCGGGGACGA 
AATCCTCCTC CTGATCTCGA GAAGGCTGTA ACTGAGTTAA CTGTGATACA GAAGGACATT 
CGTGCAATTG TCCCATTAGC AATGGACACA GAAATTATTA ATACAGCCAT TCTGACAGGA 
CGGACAGTGG CAATACCAGT CAAAGTTCTG GCTATTGAGA CATCTGGGAT TATTACTGAT 
ATCACCTCTG TTACAGAGTG CAAATCCAGC AATGAAGACA TTATCAAGGT GTCAAGCAGC 
TGTGACTATG TGTTTGTCAG CGGGAAGGAA ACCAGGGGCT CGATGAATGC ACAAGCCATG 
TTTAGCTATG AACACCTCAG TGCACCATTA GAAATGACAG TCTGGGTACC CAAAATACCA 
TTATATATTG AGCTATCTGA TTCCAAATTG AGTCAAGTAA AAGGCTGGAG AATCCCAATA 
CTGCCAGACA GAAGGTCTGT TCGGGATAGC GATGATGATG ATGATGATGA GGATGATAGA 
AAGCTCAGCC GCGGCTGTAC TTTGCAGTAC CAACATGCTA CTCTTCAAGT ATTCACCCAG 
TTTCACACAA CCTCCTCAGA GGGTACTGAC CAAGTTATCA CCATGCTGGG CCCTGATTGG 
CTGGTGGAGG TTACTGACCT AGTAAATGAC TTTATGCAGG TAGAGGACCC ACGTATTGCA 
GAAATGGTTG ACAGTAATAC AGTGGCTGGG CGTGAACCTG GAACTACATT ATTTAAAGTG 
GTGTCTCCTC TGATGGAAAC AGTGCTTGGT CAGACCCCTA TTACAGTGAC AGAGGAAAAA 
GTCAGCATAG CCGAACTTCG GGCCCAGGTG ATATCTGGCC TAACCCTCTC CCTTCATCCA 
AGTCCTGGAA ACAGCCACAC TATCATTGCA AAGACAAGTG CCCAGCACAT ACTGAAATCC 
CCTAAACAAG AAGGCCTGCT GAATCATTGG CTAACATTCA GTGATGGATC CTCTGTACCT 
CTTTCACTTT ATGATCCCAA AGACTATACA CTATCAGTGA GCAGTCTGGA TGAACGAGTG 
ATCTCAGTGA GCCCAGATCG GACCTTCCCT ATTGTAACAG CTGGAGAGGA ACAGGGCACA 
GGAACCCTCC TGCGGACTGA GATGATAATA AATGAGATCT GCCAAAAAAC AAAGAGGAAG 
AGTATTTTGT CTACAGCAAC AGCACTAGTG CAAGTCATGT TTGGTCCAGA TGAGCCAGGA 
GAAGAGGATG AACCAGGGAA ATTAGTAAGT GTTGTGCCCC CACATTTTCC TGGACAGGAA 
AACAAAAATA CATCTGGCAC AACACTGATT ACGCCACTCT CTACATCAGT TGAAGATCTG 
AGTTCACTTG GTTCTGGGGA ATATCCCTTA CCAATACCTA CAGTTTTCCC TCAGCTGCCT 
CGAGGATTGA CAGACTTAGA GATTGGCATG TACGCACTCT TGGGAGTTTT CTGCTTAGCC 
ATACTAGTCT TTCTCATAAA CTGCATAGTT TTTGTTCTTA AATACAGACA CAAGAGAGTA 
CCACCAGAAG GTCAGGCAAA TCCTGATCAT TCCCATCACT GGGTTTTCTT AGGAAATGGA 
CAACCCCTCA GGGCACGAGG GGAATTATCA CCACAACCTG AAAGTCCTGG CAACGCTTTG 
GAGGCAGGAA GTTTACCACC TCATCATTCT TCATGTTGCA GAGGTGAAGC ACGAGGAAGT 
AGCAGTAACA GTAGCTCCCA GGCCAGTGTA CAGAGCCAAT CACATGGACG GGGGGATGGA 
TCATCTGGGG GAGGTGGCTC AAGTCGAGAA GCCAGTGAGG ACCCTCTAAG CTCATCTCCC 
ACATCAAAGC GAAAGCGAGT AAAGTTTACC ACCTTCACTA GCTTGCCCAG TGAAGGCAGT 
GTATATGATG CAGTACCAGG GGCAGAGGAA AATGATATGG AGTGGGTGTG CCGAGACATG 
GGACTCCGAG ATCCCCAGGA GCTTAGAGAC TATATCAGGA GAGTCAGAGA GATGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940574.2
CDS	1282..4521
Translation	MSGRRRYPGLLCSYYYYNGYSNYHYMYCCLLFLIATRATCKVLPTNSAHPTALPPSPAAILPVSYRLSNTRLAFFLREAGPLPAWNSSTPLLQRSEPFVVFQTKELPILNVTLGPFAAGQVLPKELLQPSSTLEVPDQLTVNWKIRAYIIRTKIPSTHPVVQVLFYVAGRDWDDFSVTERLPCVRLHIFRDVRDIKSSCRLRGNLATCLAQVELPQNWFGLAVAPMGRRKSSSDGAELNEESLQIELFYTLHAPDSAGGCSNEPGTRRGGEAPTQHPLLRIGSISLYKPILVEQLQEQQLDTNVFIRLPDRPLRPGEILSIMLYLVANSTVEHFTLRVKAKKGINLQSTKSRDPQWLVNSELLTGGKHSTATVDVSRVQGMPYESEDTPYHIMQLDFEMENFTSQSVTRRIMWHVDYRGRNPPPDLEKAVTELTVIQKDIRAIVPLAMDTEIINTAILTGRTVAIPVKVLAIETSGIITDITSVTECKSSNEDIIKVSSSCDYVFVSGKETRGSMNAQAMFSYEHLSAPLEMTVWVPKIPLYIELSDSKLSQVKGWRIPILPDRRSVRDSDDDDDDEDDRKLSRGCTLQYQHATLQVFTQFHTTSSEGTDQVITMLGPDWLVEVTDLVNDFMQVEDPRIAEMVDSNTVAGREPGTTLFKVVSPLMETVLGQTPITVTEEKVSIAELRAQVISGLTLSLHPSPGNSHTIIAKTSAQHILKSPKQEGLLNHWLTFSDGSSVPLSLYDPKDYTLSVSSLDERVISVSPDRTFPIVTAGEEQGTGTLLRTEMIINEICQKTKRKSILSTATALVQVMFGPDEPGEEDEPGKLVSVVPPHFPGQENKNTSGTTLITPLSTSVEDLSSLGSGEYPLPIPTVFPQLPRGLTDLEIGMYALLGVFCLAILVFLINCIVFVLKYRHKRVPPEGQANPDHSHHWVFLGNGQPLRARGELSPQPESPGNALEAGSLPPHHSSCCRGEARGSSSNSSSQASVQSQSHGRGDGSSGGGGSSREASEDPLSSSPTSKRKRVKFTTFTSLPSEGSVYDAVPGAEENDMEWVCRDMGLRDPQELRDYIRRVREMA

Target ORF information:

RefSeq Version	XM_002940528.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane protein 132E (tmem132e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940528.5

ORF Insert Sequence:

ATGTCTGGAA GGAGGAGGTA TCCAGGACTT TTATGCAGCT ATTACTACTA CAATGGGTAT 
TCTAACTACC ACTACATGTA CTGCTGCCTT CTCTTTCTTA TTGCTACAAG GGCAACCTGC 
AAAGTACTGC CTACAAACTC AGCCCATCCC ACAGCACTTC CACCATCCCC AGCTGCCATA 
CTTCCAGTCA GCTATCGCCT GTCTAATACA CGTTTGGCCT TCTTCTTGAG AGAGGCTGGA 
CCTCTTCCAG CATGGAATTC AAGCACCCCA TTGTTACAAC GTTCAGAACC TTTTGTGGTG 
TTTCAAACAA AAGAGTTACC AATCCTTAAT GTTACTTTGG GACCTTTTGC TGCTGGGCAG 
GTACTACCAA AAGAGCTGCT GCAACCTTCA AGCACCCTAG AGGTCCCTGA CCAATTAACC 
GTAAACTGGA AAATCCGTGC CTACATAATT CGCACAAAGA TACCTTCTAC TCATCCAGTT 
GTACAAGTTC TTTTTTATGT AGCTGGCCGG GACTGGGATG ACTTTAGCGT TACAGAACGT 
CTACCCTGTG TTCGACTTCA CATTTTCCGG GATGTAAGAG ACATAAAAAG TTCTTGCCGA 
CTTCGAGGAA ACCTAGCTAC TTGCCTTGCA CAGGTTGAGC TACCACAGAA CTGGTTTGGA 
TTGGCAGTTG CACCCATGGG ACGTAGAAAA TCTTCATCTG ACGGGGCAGA ACTTAATGAA 
GAAAGCCTAC AAATAGAATT GTTTTATACT TTGCATGCTC CGGACTCGGC TGGTGGGTGT 
TCAAATGAAC CAGGAACACG TAGGGGAGGA GAAGCCCCAA CTCAACATCC CCTTCTTCGT 
ATTGGAAGTA TCAGTCTATA CAAGCCTATT CTAGTTGAGC AGTTGCAAGA ACAACAGTTG 
GATACTAATG TCTTTATCAG ACTTCCAGAC CGCCCACTAA GGCCAGGAGA GATCCTAAGC 
ATAATGCTAT ATCTGGTAGC CAATTCCACC GTAGAACACT TCACACTCAG GGTGAAGGCA 
AAGAAGGGAA TAAACCTGCA GAGTACCAAA TCAAGGGATC CCCAATGGCT GGTCAACTCT 
GAGTTGCTGA CTGGTGGAAA ACATTCCACA GCGACTGTAG ATGTGTCCCG AGTTCAAGGA 
ATGCCCTATG AGAGTGAAGA TACCCCTTAC CACATAATGC AGCTTGACTT TGAAATGGAG 
AATTTCACCA GTCAGTCAGT CACAAGACGA ATAATGTGGC ATGTAGATTA CCGGGGACGA 
AATCCTCCTC CTGATCTCGA GAAGGCTGTA ACTGAGTTAA CTGTGATACA GAAGGACATT 
CGTGCAATTG TCCCATTAGC AATGGACACA GAAATTATTA ATACAGCCAT TCTGACAGGA 
CGGACAGTGG CAATACCAGT CAAAGTTCTG GCTATTGAGA CATCTGGGAT TATTACTGAT 
ATCACCTCTG TTACAGAGTG CAAATCCAGC AATGAAGACA TTATCAAGGT GTCAAGCAGC 
TGTGACTATG TGTTTGTCAG CGGGAAGGAA ACCAGGGGCT CGATGAATGC ACAAGCCATG 
TTTAGCTATG AACACCTCAG TGCACCATTA GAAATGACAG TCTGGGTACC CAAAATACCA 
TTATATATTG AGCTATCTGA TTCCAAATTG AGTCAAGTAA AAGGCTGGAG AATCCCAATA 
CTGCCAGACA GAAGGTCTGT TCGGGATAGC GATGATGATG ATGATGATGA GGATGATAGA 
AAGCTCAGCC GCGGCTGTAC TTTGCAGTAC CAACATGCTA CTCTTCAAGT ATTCACCCAG 
TTTCACACAA CCTCCTCAGA GGGTACTGAC CAAGTTATCA CCATGCTGGG CCCTGATTGG 
CTGGTGGAGG TTACTGACCT AGTAAATGAC TTTATGCAGG TAGAGGACCC ACGTATTGCA 
GAAATGGTTG ACAGTAATAC AGTGGCTGGG CGTGAACCTG GAACTACATT ATTTAAAGTG 
GTGTCTCCTC TGATGGAAAC AGTGCTTGGT CAGACCCCTA TTACAGTGAC AGAGGAAAAA 
GTCAGCATAG CCGAACTTCG GGCCCAGGTG ATATCTGGCC TAACCCTCTC CCTTCATCCA 
AGTCCTGGAA ACAGCCACAC TATCATTGCA AAGACAAGTG CCCAGCACAT ACTGAAATCC 
CCTAAACAAG AAGGCCTGCT GAATCATTGG CTAACATTCA GTGATGGATC CTCTGTACCT 
CTTTCACTTT ATGATCCCAA AGACTATACA CTATCAGTGA GCAGTCTGGA TGAACGAGTG 
ATCTCAGTGA GCCCAGATCG GACCTTCCCT ATTGTAACAG CTGGAGAGGA ACAGGGCACA 
GGAACCCTCC TGCGGACTGA GATGATAATA AATGAGATCT GCCAAAAAAC AAAGAGGAAG 
AGTATTTTGT CTACAGCAAC AGCACTAGTG CAAGTCATGT TTGGTCCAGA TGAGCCAGGA 
GAAGAGGATG AACCAGGGAA ATTAGTAAGT GTTGTGCCCC CACATTTTCC TGGACAGGAA 
AACAAAAATA CATCTGGCAC AACACTGATT ACGCCACTCT CTACATCAGT TGAAGATCTG 
AGTTCACTTG GTTCTGGGGA ATATCCCTTA CCAATACCTA CAGTTTTCCC TCAGCTGCCT 
CGAGGATTGA CAGACTTAGA GATTGGCATG TACGCACTCT TGGGAGTTTT CTGCTTAGCC 
ATACTAGTCT TTCTCATAAA CTGCATAGTT TTTGTTCTTA AATACAGACA CAAGAGAGTA 
CCACCAGAAG GTCAGGCAAA TCCTGATCAT TCCCATCACT GGGTTTTCTT AGGAAATGGA 
CAACCCCTCA GGGCACGAGG GGAATTATCA CCACAACCTG AAAGTCCTGG CAACGCTTTG 
GAGGCAGGAA GTTTACCACC TCATCATTCT TCATGTTGCA GAGGTGAAGC ACGAGGAAGT 
AGCAGTAACA GTAGCTCCCA GGCCAGTGTA CAGAGCCAAT CACATGGACG GGGGGATGGA 
TCATCTGGGG GAGGTGGCTC AAGTCGAGAA GCCAGTGAGG ACCCTCTAAG CTCATCTCCC 
ACATCAAAGC GAAAGCGAGT AAAGTTTACC ACCTTCACTA GCTTGCCCAG TGAAGGCAGT 
GTATATGATG CAGTACCAGG GGCAGAGGAA AATGATATGG AGTGGGTGTG CCGAGACATG 
GGACTCCGAG ATCCCCAGGA GCTTAGAGAC TATATCAGGA GAGTCAGAGA GATGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmem132e ( XM_002940528.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940574.2 Xenopus tropicalis transmembrane protein 132E (tmem132e), mRNA.

Price & Availability↑

CloneID	OXa05637
Clone ID Related Accession (Same CDS sequence)	XM_002940528.4 , XM_002940528.5
Accession Version	XM_002940528.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transmembrane protein 132E
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940528.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAA GGAGGAGGTA TCCAGGACTT TTATGCAGCT ATTACTACTA CAATGGGTAT 
TCTAACTACC ACTACATGTA CTGCTGCCTT CTCTTTCTTA TTGCTACAAG GGCAACCTGC 
AAAGTACTGC CTACAAACTC AGCCCATCCC ACAGCACTTC CACCATCCCC AGCTGCCATA 
CTTCCAGTCA GCTATCGCCT GTCTAATACA CGTTTGGCCT TCTTCTTGAG AGAGGCTGGA 
CCTCTTCCAG CATGGAATTC AAGCACCCCA TTGTTACAAC GTTCAGAACC TTTTGTGGTG 
TTTCAAACAA AAGAGTTACC AATCCTTAAT GTTACTTTGG GACCTTTTGC TGCTGGGCAG 
GTACTACCAA AAGAGCTGCT GCAACCTTCA AGCACCCTAG AGGTCCCTGA CCAATTAACC 
GTAAACTGGA AAATCCGTGC CTACATAATT CGCACAAAGA TACCTTCTAC TCATCCAGTT 
GTACAAGTTC TTTTTTATGT AGCTGGCCGG GACTGGGATG ACTTTAGCGT TACAGAACGT 
CTACCCTGTG TTCGACTTCA CATTTTCCGG GATGTAAGAG ACATAAAAAG TTCTTGCCGA 
CTTCGAGGAA ACCTAGCTAC TTGCCTTGCA CAGGTTGAGC TACCACAGAA CTGGTTTGGA 
TTGGCAGTTG CACCCATGGG ACGTAGAAAA TCTTCATCTG ACGGGGCAGA ACTTAATGAA 
GAAAGCCTAC AAATAGAATT GTTTTATACT TTGCATGCTC CGGACTCGGC TGGTGGGTGT 
TCAAATGAAC CAGGAACACG TAGGGGAGGA GAAGCCCCAA CTCAACATCC CCTTCTTCGT 
ATTGGAAGTA TCAGTCTATA CAAGCCTATT CTAGTTGAGC AGTTGCAAGA ACAACAGTTG 
GATACTAATG TCTTTATCAG ACTTCCAGAC CGCCCACTAA GGCCAGGAGA GATCCTAAGC 
ATAATGCTAT ATCTGGTAGC CAATTCCACC GTAGAACACT TCACACTCAG GGTGAAGGCA 
AAGAAGGGAA TAAACCTGCA GAGTACCAAA TCAAGGGATC CCCAATGGCT GGTCAACTCT 
GAGTTGCTGA CTGGTGGAAA ACATTCCACA GCGACTGTAG ATGTGTCCCG AGTTCAAGGA 
ATGCCCTATG AGAGTGAAGA TACCCCTTAC CACATAATGC AGCTTGACTT TGAAATGGAG 
AATTTCACCA GTCAGTCAGT CACAAGACGA ATAATGTGGC ATGTAGATTA CCGGGGACGA 
AATCCTCCTC CTGATCTCGA GAAGGCTGTA ACTGAGTTAA CTGTGATACA GAAGGACATT 
CGTGCAATTG TCCCATTAGC AATGGACACA GAAATTATTA ATACAGCCAT TCTGACAGGA 
CGGACAGTGG CAATACCAGT CAAAGTTCTG GCTATTGAGA CATCTGGGAT TATTACTGAT 
ATCACCTCTG TTACAGAGTG CAAATCCAGC AATGAAGACA TTATCAAGGT GTCAAGCAGC 
TGTGACTATG TGTTTGTCAG CGGGAAGGAA ACCAGGGGCT CGATGAATGC ACAAGCCATG 
TTTAGCTATG AACACCTCAG TGCACCATTA GAAATGACAG TCTGGGTACC CAAAATACCA 
TTATATATTG AGCTATCTGA TTCCAAATTG AGTCAAGTAA AAGGCTGGAG AATCCCAATA 
CTGCCAGACA GAAGGTCTGT TCGGGATAGC GATGATGATG ATGATGATGA GGATGATAGA 
AAGCTCAGCC GCGGCTGTAC TTTGCAGTAC CAACATGCTA CTCTTCAAGT ATTCACCCAG 
TTTCACACAA CCTCCTCAGA GGGTACTGAC CAAGTTATCA CCATGCTGGG CCCTGATTGG 
CTGGTGGAGG TTACTGACCT AGTAAATGAC TTTATGCAGG TAGAGGACCC ACGTATTGCA 
GAAATGGTTG ACAGTAATAC AGTGGCTGGG CGTGAACCTG GAACTACATT ATTTAAAGTG 
GTGTCTCCTC TGATGGAAAC AGTGCTTGGT CAGACCCCTA TTACAGTGAC AGAGGAAAAA 
GTCAGCATAG CCGAACTTCG GGCCCAGGTG ATATCTGGCC TAACCCTCTC CCTTCATCCA 
AGTCCTGGAA ACAGCCACAC TATCATTGCA AAGACAAGTG CCCAGCACAT ACTGAAATCC 
CCTAAACAAG AAGGCCTGCT GAATCATTGG CTAACATTCA GTGATGGATC CTCTGTACCT 
CTTTCACTTT ATGATCCCAA AGACTATACA CTATCAGTGA GCAGTCTGGA TGAACGAGTG 
ATCTCAGTGA GCCCAGATCG GACCTTCCCT ATTGTAACAG CTGGAGAGGA ACAGGGCACA 
GGAACCCTCC TGCGGACTGA GATGATAATA AATGAGATCT GCCAAAAAAC AAAGAGGAAG 
AGTATTTTGT CTACAGCAAC AGCACTAGTG CAAGTCATGT TTGGTCCAGA TGAGCCAGGA 
GAAGAGGATG AACCAGGGAA ATTAGTAAGT GTTGTGCCCC CACATTTTCC TGGACAGGAA 
AACAAAAATA CATCTGGCAC AACACTGATT ACGCCACTCT CTACATCAGT TGAAGATCTG 
AGTTCACTTG GTTCTGGGGA ATATCCCTTA CCAATACCTA CAGTTTTCCC TCAGCTGCCT 
CGAGGATTGA CAGACTTAGA GATTGGCATG TACGCACTCT TGGGAGTTTT CTGCTTAGCC 
ATACTAGTCT TTCTCATAAA CTGCATAGTT TTTGTTCTTA AATACAGACA CAAGAGAGTA 
CCACCAGAAG GTCAGGCAAA TCCTGATCAT TCCCATCACT GGGTTTTCTT AGGAAATGGA 
CAACCCCTCA GGGCACGAGG GGAATTATCA CCACAACCTG AAAGTCCTGG CAACGCTTTG 
GAGGCAGGAA GTTTACCACC TCATCATTCT TCATGTTGCA GAGGTGAAGC ACGAGGAAGT 
AGCAGTAACA GTAGCTCCCA GGCCAGTGTA CAGAGCCAAT CACATGGACG GGGGGATGGA 
TCATCTGGGG GAGGTGGCTC AAGTCGAGAA GCCAGTGAGG ACCCTCTAAG CTCATCTCCC 
ACATCAAAGC GAAAGCGAGT AAAGTTTACC ACCTTCACTA GCTTGCCCAG TGAAGGCAGT 
GTATATGATG CAGTACCAGG GGCAGAGGAA AATGATATGG AGTGGGTGTG CCGAGACATG 
GGACTCCGAG ATCCCCAGGA GCTTAGAGAC TATATCAGGA GAGTCAGAGA GATGGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940574.2
CDS	1889..5128
Translation	MSGRRRYPGLLCSYYYYNGYSNYHYMYCCLLFLIATRATCKVLPTNSAHPTALPPSPAAILPVSYRLSNTRLAFFLREAGPLPAWNSSTPLLQRSEPFVVFQTKELPILNVTLGPFAAGQVLPKELLQPSSTLEVPDQLTVNWKIRAYIIRTKIPSTHPVVQVLFYVAGRDWDDFSVTERLPCVRLHIFRDVRDIKSSCRLRGNLATCLAQVELPQNWFGLAVAPMGRRKSSSDGAELNEESLQIELFYTLHAPDSAGGCSNEPGTRRGGEAPTQHPLLRIGSISLYKPILVEQLQEQQLDTNVFIRLPDRPLRPGEILSIMLYLVANSTVEHFTLRVKAKKGINLQSTKSRDPQWLVNSELLTGGKHSTATVDVSRVQGMPYESEDTPYHIMQLDFEMENFTSQSVTRRIMWHVDYRGRNPPPDLEKAVTELTVIQKDIRAIVPLAMDTEIINTAILTGRTVAIPVKVLAIETSGIITDITSVTECKSSNEDIIKVSSSCDYVFVSGKETRGSMNAQAMFSYEHLSAPLEMTVWVPKIPLYIELSDSKLSQVKGWRIPILPDRRSVRDSDDDDDDEDDRKLSRGCTLQYQHATLQVFTQFHTTSSEGTDQVITMLGPDWLVEVTDLVNDFMQVEDPRIAEMVDSNTVAGREPGTTLFKVVSPLMETVLGQTPITVTEEKVSIAELRAQVISGLTLSLHPSPGNSHTIIAKTSAQHILKSPKQEGLLNHWLTFSDGSSVPLSLYDPKDYTLSVSSLDERVISVSPDRTFPIVTAGEEQGTGTLLRTEMIINEICQKTKRKSILSTATALVQVMFGPDEPGEEDEPGKLVSVVPPHFPGQENKNTSGTTLITPLSTSVEDLSSLGSGEYPLPIPTVFPQLPRGLTDLEIGMYALLGVFCLAILVFLINCIVFVLKYRHKRVPPEGQANPDHSHHWVFLGNGQPLRARGELSPQPESPGNALEAGSLPPHHSSCCRGEARGSSSNSSSQASVQSQSHGRGDGSSGGGGSSREASEDPLSSSPTSKRKRVKFTTFTSLPSEGSVYDAVPGAEENDMEWVCRDMGLRDPQELRDYIRRVREMA

Target ORF information:

RefSeq Version	XM_002940528.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis transmembrane protein 132E (tmem132e), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940528.4

ORF Insert Sequence:

ATGTCTGGAA GGAGGAGGTA TCCAGGACTT TTATGCAGCT ATTACTACTA CAATGGGTAT 
TCTAACTACC ACTACATGTA CTGCTGCCTT CTCTTTCTTA TTGCTACAAG GGCAACCTGC 
AAAGTACTGC CTACAAACTC AGCCCATCCC ACAGCACTTC CACCATCCCC AGCTGCCATA 
CTTCCAGTCA GCTATCGCCT GTCTAATACA CGTTTGGCCT TCTTCTTGAG AGAGGCTGGA 
CCTCTTCCAG CATGGAATTC AAGCACCCCA TTGTTACAAC GTTCAGAACC TTTTGTGGTG 
TTTCAAACAA AAGAGTTACC AATCCTTAAT GTTACTTTGG GACCTTTTGC TGCTGGGCAG 
GTACTACCAA AAGAGCTGCT GCAACCTTCA AGCACCCTAG AGGTCCCTGA CCAATTAACC 
GTAAACTGGA AAATCCGTGC CTACATAATT CGCACAAAGA TACCTTCTAC TCATCCAGTT 
GTACAAGTTC TTTTTTATGT AGCTGGCCGG GACTGGGATG ACTTTAGCGT TACAGAACGT 
CTACCCTGTG TTCGACTTCA CATTTTCCGG GATGTAAGAG ACATAAAAAG TTCTTGCCGA 
CTTCGAGGAA ACCTAGCTAC TTGCCTTGCA CAGGTTGAGC TACCACAGAA CTGGTTTGGA 
TTGGCAGTTG CACCCATGGG ACGTAGAAAA TCTTCATCTG ACGGGGCAGA ACTTAATGAA 
GAAAGCCTAC AAATAGAATT GTTTTATACT TTGCATGCTC CGGACTCGGC TGGTGGGTGT 
TCAAATGAAC CAGGAACACG TAGGGGAGGA GAAGCCCCAA CTCAACATCC CCTTCTTCGT 
ATTGGAAGTA TCAGTCTATA CAAGCCTATT CTAGTTGAGC AGTTGCAAGA ACAACAGTTG 
GATACTAATG TCTTTATCAG ACTTCCAGAC CGCCCACTAA GGCCAGGAGA GATCCTAAGC 
ATAATGCTAT ATCTGGTAGC CAATTCCACC GTAGAACACT TCACACTCAG GGTGAAGGCA 
AAGAAGGGAA TAAACCTGCA GAGTACCAAA TCAAGGGATC CCCAATGGCT GGTCAACTCT 
GAGTTGCTGA CTGGTGGAAA ACATTCCACA GCGACTGTAG ATGTGTCCCG AGTTCAAGGA 
ATGCCCTATG AGAGTGAAGA TACCCCTTAC CACATAATGC AGCTTGACTT TGAAATGGAG 
AATTTCACCA GTCAGTCAGT CACAAGACGA ATAATGTGGC ATGTAGATTA CCGGGGACGA 
AATCCTCCTC CTGATCTCGA GAAGGCTGTA ACTGAGTTAA CTGTGATACA GAAGGACATT 
CGTGCAATTG TCCCATTAGC AATGGACACA GAAATTATTA ATACAGCCAT TCTGACAGGA 
CGGACAGTGG CAATACCAGT CAAAGTTCTG GCTATTGAGA CATCTGGGAT TATTACTGAT 
ATCACCTCTG TTACAGAGTG CAAATCCAGC AATGAAGACA TTATCAAGGT GTCAAGCAGC 
TGTGACTATG TGTTTGTCAG CGGGAAGGAA ACCAGGGGCT CGATGAATGC ACAAGCCATG 
TTTAGCTATG AACACCTCAG TGCACCATTA GAAATGACAG TCTGGGTACC CAAAATACCA 
TTATATATTG AGCTATCTGA TTCCAAATTG AGTCAAGTAA AAGGCTGGAG AATCCCAATA 
CTGCCAGACA GAAGGTCTGT TCGGGATAGC GATGATGATG ATGATGATGA GGATGATAGA 
AAGCTCAGCC GCGGCTGTAC TTTGCAGTAC CAACATGCTA CTCTTCAAGT ATTCACCCAG 
TTTCACACAA CCTCCTCAGA GGGTACTGAC CAAGTTATCA CCATGCTGGG CCCTGATTGG 
CTGGTGGAGG TTACTGACCT AGTAAATGAC TTTATGCAGG TAGAGGACCC ACGTATTGCA 
GAAATGGTTG ACAGTAATAC AGTGGCTGGG CGTGAACCTG GAACTACATT ATTTAAAGTG 
GTGTCTCCTC TGATGGAAAC AGTGCTTGGT CAGACCCCTA TTACAGTGAC AGAGGAAAAA 
GTCAGCATAG CCGAACTTCG GGCCCAGGTG ATATCTGGCC TAACCCTCTC CCTTCATCCA 
AGTCCTGGAA ACAGCCACAC TATCATTGCA AAGACAAGTG CCCAGCACAT ACTGAAATCC 
CCTAAACAAG AAGGCCTGCT GAATCATTGG CTAACATTCA GTGATGGATC CTCTGTACCT 
CTTTCACTTT ATGATCCCAA AGACTATACA CTATCAGTGA GCAGTCTGGA TGAACGAGTG 
ATCTCAGTGA GCCCAGATCG GACCTTCCCT ATTGTAACAG CTGGAGAGGA ACAGGGCACA 
GGAACCCTCC TGCGGACTGA GATGATAATA AATGAGATCT GCCAAAAAAC AAAGAGGAAG 
AGTATTTTGT CTACAGCAAC AGCACTAGTG CAAGTCATGT TTGGTCCAGA TGAGCCAGGA 
GAAGAGGATG AACCAGGGAA ATTAGTAAGT GTTGTGCCCC CACATTTTCC TGGACAGGAA 
AACAAAAATA CATCTGGCAC AACACTGATT ACGCCACTCT CTACATCAGT TGAAGATCTG 
AGTTCACTTG GTTCTGGGGA ATATCCCTTA CCAATACCTA CAGTTTTCCC TCAGCTGCCT 
CGAGGATTGA CAGACTTAGA GATTGGCATG TACGCACTCT TGGGAGTTTT CTGCTTAGCC 
ATACTAGTCT TTCTCATAAA CTGCATAGTT TTTGTTCTTA AATACAGACA CAAGAGAGTA 
CCACCAGAAG GTCAGGCAAA TCCTGATCAT TCCCATCACT GGGTTTTCTT AGGAAATGGA 
CAACCCCTCA GGGCACGAGG GGAATTATCA CCACAACCTG AAAGTCCTGG CAACGCTTTG 
GAGGCAGGAA GTTTACCACC TCATCATTCT TCATGTTGCA GAGGTGAAGC ACGAGGAAGT 
AGCAGTAACA GTAGCTCCCA GGCCAGTGTA CAGAGCCAAT CACATGGACG GGGGGATGGA 
TCATCTGGGG GAGGTGGCTC AAGTCGAGAA GCCAGTGAGG ACCCTCTAAG CTCATCTCCC 
ACATCAAAGC GAAAGCGAGT AAAGTTTACC ACCTTCACTA GCTTGCCCAG TGAAGGCAGT 
GTATATGATG CAGTACCAGG GGCAGAGGAA AATGATATGG AGTGGGTGTG CCGAGACATG 
GGACTCCGAG ATCCCCAGGA GCTTAGAGAC TATATCAGGA GAGTCAGAGA GATGGCATAG