abcf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	abcf1
Entrez Gene ID	100490104
Full Name	ATP binding cassette subfamily F member 1
General protein information	Preferred Names ATP binding cassette subfamily F member 1 Names ATP-binding cassette sub-family F member 1 ATP-binding cassette, sub-family F (GCN20), member 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002939468.5	XP_002939514.2	ATP-binding cassette sub-family F member 1
XM_002939468.4	XP_002939514.2	ATP-binding cassette sub-family F member 1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os03g0441500	NP_001050461.1
Mus musculus (house mouse)	Abcf1	NP_038882.1
Arabidopsis thaliana (thale cress)	GCN4	NP_567001.1
Rattus norvegicus (Norway rat)	Abcf1	NP_001103353.1
Danio rerio (zebrafish)	abcf1	NP_998351.1
Drosophila melanogaster (fruit fly)	CG1703	NP_572736.1
	ABCF1	NP_001098632.1
Canis lupus familiaris (dog)	ABCF1	XP_532056.4
Caenorhabditis elegans (roundworm)	abcf-1	NP_506192.1
Homo sapiens (human)	ABCF1	NP_001081.1
Pan troglodytes (chimpanzee)	ABCF1	NP_001035838.1
Bos taurus (cattle)	ABCF1	NP_001193838.1
Xenopus tropicalis (tropical clawed frog)	abcf1	XP_002939514.2

Gene Ontology

The following abcf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the abcf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa14437	XM_002939468.5 Latest version!	Xenopus tropicalis ATP binding cassette subfamily F member 1 (abcf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa14437	XM_002939468.4	Xenopus tropicalis ATP binding cassette subfamily F member 1 (abcf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

abcf1 ( XM_002939468.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939514.2 Xenopus tropicalis ATP binding cassette subfamily F member 1 (abcf1), mRNA.

Price & Availability↑

CloneID	OXa14437
Clone ID Related Accession (Same CDS sequence)	XM_002939468.5 , XM_002939468.4
Accession Version	XM_002939468.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-binding cassette sub-family F member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939468.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGCG GAGGAAAGCA TGAGGTTGGA GAAGAAGAGG AGGAGGAGGA GGTGAAAGGG 
AAGGAAACAG AAAAAGTGGT GAAGAAAGGA AAAAAGGACA AGAAAGGGAA AAAATCGTTT 
TTTGAGGAGC TGTCTGCTGA TAACAAGCAG GCACAGGAAG AGGAGGCTGC GAAGGAACGG 
GAAAAGGAAA AGGAGTTGCA ACAGCAACAG CAAAAAAAGA GAGAAAAAAA GAAAAAAGAT 
CGTCGGAAGC AAAAAGGAGA CGAAGATGAT GATGATGAAG AAAAGAAAGA AAGAGAAATT 
GCAGAGAGGC TAAAAAAGCT GTCAGTGCAA GCAAGTGATG AAGATGAAGA ACCAGATGTA 
AAACCTGCAC CCAAAGCTGG GAAGAAGCAG GGGAGTAATA TCTTTGCAGC CCTAGTAGAG 
GATCAAAGTG ATGACGAAGA AGAAGAAGAG CAGGTATCCA ACCAGGTGGA TGAGAAAAAA 
CAAAGTAAAG GGAAGAAAAA TGTGAAAGGA AAACATAAGG CTCCTGAAGA AAGACCAGAA 
AGTGAGGAGG ATATTCCTAA GGAGAAATCA AAGGGCAAAA AACCTGTCCC CAGTGCTGCT 
TCTGTTAATG AAGAAGATGA GAAAAAAACT GAGAAGGGGA ATAAGAAATC AAAACCAAGT 
CAGAAACCTG TCTCCAAGGC TGCATCTGTT GAGTCTGATG AAGAGAAAAA ACCTGAGAAG 
GGGAGTAAAA AAACAAAACC AAGCCAGAAT CGGTTTGGTG CCTTAGACAT AGAGGAAGAT 
CATGTCAGTG GAGGATCAAA AGAAGGGTCA GGTGATGAGG GTGAGACAGA AGACAAGACA 
ACCGCTGCTG AAGATCCTTT TGCCAATTTA AGCAAAAAGG AGAAAAAGAA GAAAAAAAAA 
ATGCTGGAAT ATGAGAAACA AGTGGCTACT ATGAAGAAGA TAACTGCTGC AGAAAATGAC 
TTCTCTGTGT CACAAGCAGA ACTGTCATCA CGACAAGCCA TGCTGGAAAA TGCTTCAGAT 
ATAAAGATAG AAAAATTCAG CATTTCTGCC CACGGGAAGG AGTTGTTTGT TAATGCCGAT 
TTATATGTGG TAGCAGGTCG GCGCTATGGA TTGGTTGGGC CTAATGGGAA AGGCAAAACC 
ACTCTCCTAA AACACATAGC AAACAAGGCA CTGAACATAC CCCCAAACAT TGATGTCTTG 
CTGTGTGAGC AAGAGGTTGT TGCTGATGAC ACACCTGCAG TCCAGGCTGT GCTTAAAGCT 
GATAAGAAGC GACTAAAGTT GCTAGAGGAG GAGAGAAAAC TGCAAACCCG ACTTGAAAAG 
GGGGATGACA ATGCTGCCGA AAGATTAGAA AAGGTGTATG AGGAGTTAAG AGCTATGGGG 
GCTGCATCTG CCGAGGCCAA GGCGCGCAGG ATTCTGGCTG GTCTTGGCTT CACGCCTGAA 
ATGCAGGACA GAGAAACCCG TCGATTTAGT GGAGGTTGGA GAATGAGAGT CTCTTTGGCC 
AGAGCTCTCT TCATGGAGCC AACTCTACTG ATGCTGGATG AACCTACCAA TCACTTGGAT 
CTCAATGCTG TTATTTGGCT TAACAATTAC CTTCAGGGCT GGAAGAAAAC TCTTTTAATT 
GTGTCCCACG ATCAAGGGTT CTTAGATGAT GTCTGTACGG ATATAATGCA CCTGGATTCA 
CAGAAGCTGT ATTATTATCG AGGCAACTAC ATGACATTTA AGAAGATGTA CCAGCAAAAG 
CAGAAGGAAC TGCAGAAACA GTATGACAAG CAAGAGAAAA AACTCAAAGA CTTAAAAGCA 
GGAGGAAAGT CAGCTAAACA AGCGGAGAAG CAGACAAAAG AGGCACTAAC TCGCAAGCAG 
CAGAAGTGTC AGAAGAAGAA CAATGACAAG GAAGACAATG ATCCTGTTGA ATTACTAAAG 
AGGCCCAAGG AATATACTGT GAAATTCACT TTTCCAAATC CACCTCCACT CAGCCCTCCA 
ATTTTGGGTC TTCATGGTGT GGATTTTTGT TATTCTGGAC AAAAGCCACT GTTCAAAAAC 
TTGGATTTTG GCATAGACAT GGACTCTAGA GTGTGTATTG TGGGCCCCAA TGGAGTAGGG 
AAGAGTACAC TGCTCTTGCT GCTGACTGGA AAACTTACTC CTACCAAAGG AGAAATGAGA 
AAAAATCACA GACTGAAAAT AGGATTCTTT AACCAGCAAT ATGCAGATCA GCTAAACATG 
GAGGAGACAG CTACAGAATA TCTACAACGC AATTTCAATC TTCCATATCA GGATGCACGC 
AAATGTCTGG GAAGGTTTGG TCTAGAAAGC CATGCACATA CCATCCAGAT CTGCAAGCTG 
TCAGGAGGGC AGAAAGCTAG AGTGGTTTTT GCTGAGCTGT GTTGCAGGGA ACCTGACGTA 
TTAATTTTAG ATGAACCCAC CAATAACCTA GATATAGAGT CAATAGATGC TCTTGCGGAA 
GCGGTGAATG AATATAAAGG AGCTGTTATC ACAGTGAGCC ATGACGCCCG GCTTATTACA 
GAGACGAACT GTCACTTGTG GGTGGTAGAG GACCAGACTG TAAATCAAAT AGAGGGTGAT 
TTTGATGACT ATAAGAGAGA GGTCTTGGAG AGCCTGGGAG AAGTGATAGT GAACAAACCA 
CGGGAGAGGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939514.2
CDS	186..2840
Translation	MPRGGKHEVGEEEEEEEVKGKETEKVVKKGKKDKKGKKSFFEELSADNKQAQEEEAAKEREKEKELQQQQQKKREKKKKDRRKQKGDEDDDDEEKKEREIAERLKKLSVQASDEDEEPDVKPAPKAGKKQGSNIFAALVEDQSDDEEEEEQVSNQVDEKKQSKGKKNVKGKHKAPEERPESEEDIPKEKSKGKKPVPSAASVNEEDEKKTEKGNKKSKPSQKPVSKAASVESDEEKKPEKGSKKTKPSQNRFGALDIEEDHVSGGSKEGSGDEGETEDKTTAAEDPFANLSKKEKKKKKKMLEYEKQVATMKKITAAENDFSVSQAELSSRQAMLENASDIKIEKFSISAHGKELFVNADLYVVAGRRYGLVGPNGKGKTTLLKHIANKALNIPPNIDVLLCEQEVVADDTPAVQAVLKADKKRLKLLEEERKLQTRLEKGDDNAAERLEKVYEELRAMGAASAEAKARRILAGLGFTPEMQDRETRRFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWKKTLLIVSHDQGFLDDVCTDIMHLDSQKLYYYRGNYMTFKKMYQQKQKELQKQYDKQEKKLKDLKAGGKSAKQAEKQTKEALTRKQQKCQKKNNDKEDNDPVELLKRPKEYTVKFTFPNPPPLSPPILGLHGVDFCYSGQKPLFKNLDFGIDMDSRVCIVGPNGVGKSTLLLLLTGKLTPTKGEMRKNHRLKIGFFNQQYADQLNMEETATEYLQRNFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELCCREPDVLILDEPTNNLDIESIDALAEAVNEYKGAVITVSHDARLITETNCHLWVVEDQTVNQIEGDFDDYKREVLESLGEVIVNKPRERD

Target ORF information:

RefSeq Version	XM_002939468.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP binding cassette subfamily F member 1 (abcf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939468.4

ORF Insert Sequence:

ATGCCTCGCG GAGGAAAGCA TGAGGTTGGA GAAGAAGAGG AGGAGGAGGA GGTGAAAGGG 
AAGGAAACAG AAAAAGTGGT GAAGAAAGGA AAAAAGGACA AGAAAGGGAA AAAATCGTTT 
TTTGAGGAGC TGTCTGCTGA TAACAAGCAG GCACAGGAAG AGGAGGCTGC GAAGGAACGG 
GAAAAGGAAA AGGAGTTGCA ACAGCAACAG CAAAAAAAGA GAGAAAAAAA GAAAAAAGAT 
CGTCGGAAGC AAAAAGGAGA CGAAGATGAT GATGATGAAG AAAAGAAAGA AAGAGAAATT 
GCAGAGAGGC TAAAAAAGCT GTCAGTGCAA GCAAGTGATG AAGATGAAGA ACCAGATGTA 
AAACCTGCAC CCAAAGCTGG GAAGAAGCAG GGGAGTAATA TCTTTGCAGC CCTAGTAGAG 
GATCAAAGTG ATGACGAAGA AGAAGAAGAG CAGGTATCCA ACCAGGTGGA TGAGAAAAAA 
CAAAGTAAAG GGAAGAAAAA TGTGAAAGGA AAACATAAGG CTCCTGAAGA AAGACCAGAA 
AGTGAGGAGG ATATTCCTAA GGAGAAATCA AAGGGCAAAA AACCTGTCCC CAGTGCTGCT 
TCTGTTAATG AAGAAGATGA GAAAAAAACT GAGAAGGGGA ATAAGAAATC AAAACCAAGT 
CAGAAACCTG TCTCCAAGGC TGCATCTGTT GAGTCTGATG AAGAGAAAAA ACCTGAGAAG 
GGGAGTAAAA AAACAAAACC AAGCCAGAAT CGGTTTGGTG CCTTAGACAT AGAGGAAGAT 
CATGTCAGTG GAGGATCAAA AGAAGGGTCA GGTGATGAGG GTGAGACAGA AGACAAGACA 
ACCGCTGCTG AAGATCCTTT TGCCAATTTA AGCAAAAAGG AGAAAAAGAA GAAAAAAAAA 
ATGCTGGAAT ATGAGAAACA AGTGGCTACT ATGAAGAAGA TAACTGCTGC AGAAAATGAC 
TTCTCTGTGT CACAAGCAGA ACTGTCATCA CGACAAGCCA TGCTGGAAAA TGCTTCAGAT 
ATAAAGATAG AAAAATTCAG CATTTCTGCC CACGGGAAGG AGTTGTTTGT TAATGCCGAT 
TTATATGTGG TAGCAGGTCG GCGCTATGGA TTGGTTGGGC CTAATGGGAA AGGCAAAACC 
ACTCTCCTAA AACACATAGC AAACAAGGCA CTGAACATAC CCCCAAACAT TGATGTCTTG 
CTGTGTGAGC AAGAGGTTGT TGCTGATGAC ACACCTGCAG TCCAGGCTGT GCTTAAAGCT 
GATAAGAAGC GACTAAAGTT GCTAGAGGAG GAGAGAAAAC TGCAAACCCG ACTTGAAAAG 
GGGGATGACA ATGCTGCCGA AAGATTAGAA AAGGTGTATG AGGAGTTAAG AGCTATGGGG 
GCTGCATCTG CCGAGGCCAA GGCGCGCAGG ATTCTGGCTG GTCTTGGCTT CACGCCTGAA 
ATGCAGGACA GAGAAACCCG TCGATTTAGT GGAGGTTGGA GAATGAGAGT CTCTTTGGCC 
AGAGCTCTCT TCATGGAGCC AACTCTACTG ATGCTGGATG AACCTACCAA TCACTTGGAT 
CTCAATGCTG TTATTTGGCT TAACAATTAC CTTCAGGGCT GGAAGAAAAC TCTTTTAATT 
GTGTCCCACG ATCAAGGGTT CTTAGATGAT GTCTGTACGG ATATAATGCA CCTGGATTCA 
CAGAAGCTGT ATTATTATCG AGGCAACTAC ATGACATTTA AGAAGATGTA CCAGCAAAAG 
CAGAAGGAAC TGCAGAAACA GTATGACAAG CAAGAGAAAA AACTCAAAGA CTTAAAAGCA 
GGAGGAAAGT CAGCTAAACA AGCGGAGAAG CAGACAAAAG AGGCACTAAC TCGCAAGCAG 
CAGAAGTGTC AGAAGAAGAA CAATGACAAG GAAGACAATG ATCCTGTTGA ATTACTAAAG 
AGGCCCAAGG AATATACTGT GAAATTCACT TTTCCAAATC CACCTCCACT CAGCCCTCCA 
ATTTTGGGTC TTCATGGTGT GGATTTTTGT TATTCTGGAC AAAAGCCACT GTTCAAAAAC 
TTGGATTTTG GCATAGACAT GGACTCTAGA GTGTGTATTG TGGGCCCCAA TGGAGTAGGG 
AAGAGTACAC TGCTCTTGCT GCTGACTGGA AAACTTACTC CTACCAAAGG AGAAATGAGA 
AAAAATCACA GACTGAAAAT AGGATTCTTT AACCAGCAAT ATGCAGATCA GCTAAACATG 
GAGGAGACAG CTACAGAATA TCTACAACGC AATTTCAATC TTCCATATCA GGATGCACGC 
AAATGTCTGG GAAGGTTTGG TCTAGAAAGC CATGCACATA CCATCCAGAT CTGCAAGCTG 
TCAGGAGGGC AGAAAGCTAG AGTGGTTTTT GCTGAGCTGT GTTGCAGGGA ACCTGACGTA 
TTAATTTTAG ATGAACCCAC CAATAACCTA GATATAGAGT CAATAGATGC TCTTGCGGAA 
GCGGTGAATG AATATAAAGG AGCTGTTATC ACAGTGAGCC ATGACGCCCG GCTTATTACA 
GAGACGAACT GTCACTTGTG GGTGGTAGAG GACCAGACTG TAAATCAAAT AGAGGGTGAT 
TTTGATGACT ATAAGAGAGA GGTCTTGGAG AGCCTGGGAG AAGTGATAGT GAACAAACCA 
CGGGAGAGGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

abcf1 ( XM_002939468.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939514.2 Xenopus tropicalis ATP binding cassette subfamily F member 1 (abcf1), mRNA.

Price & Availability↑

CloneID	OXa14437
Clone ID Related Accession (Same CDS sequence)	XM_002939468.5 , XM_002939468.4
Accession Version	XM_002939468.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ATP-binding cassette sub-family F member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939468.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCGCG GAGGAAAGCA TGAGGTTGGA GAAGAAGAGG AGGAGGAGGA GGTGAAAGGG 
AAGGAAACAG AAAAAGTGGT GAAGAAAGGA AAAAAGGACA AGAAAGGGAA AAAATCGTTT 
TTTGAGGAGC TGTCTGCTGA TAACAAGCAG GCACAGGAAG AGGAGGCTGC GAAGGAACGG 
GAAAAGGAAA AGGAGTTGCA ACAGCAACAG CAAAAAAAGA GAGAAAAAAA GAAAAAAGAT 
CGTCGGAAGC AAAAAGGAGA CGAAGATGAT GATGATGAAG AAAAGAAAGA AAGAGAAATT 
GCAGAGAGGC TAAAAAAGCT GTCAGTGCAA GCAAGTGATG AAGATGAAGA ACCAGATGTA 
AAACCTGCAC CCAAAGCTGG GAAGAAGCAG GGGAGTAATA TCTTTGCAGC CCTAGTAGAG 
GATCAAAGTG ATGACGAAGA AGAAGAAGAG CAGGTATCCA ACCAGGTGGA TGAGAAAAAA 
CAAAGTAAAG GGAAGAAAAA TGTGAAAGGA AAACATAAGG CTCCTGAAGA AAGACCAGAA 
AGTGAGGAGG ATATTCCTAA GGAGAAATCA AAGGGCAAAA AACCTGTCCC CAGTGCTGCT 
TCTGTTAATG AAGAAGATGA GAAAAAAACT GAGAAGGGGA ATAAGAAATC AAAACCAAGT 
CAGAAACCTG TCTCCAAGGC TGCATCTGTT GAGTCTGATG AAGAGAAAAA ACCTGAGAAG 
GGGAGTAAAA AAACAAAACC AAGCCAGAAT CGGTTTGGTG CCTTAGACAT AGAGGAAGAT 
CATGTCAGTG GAGGATCAAA AGAAGGGTCA GGTGATGAGG GTGAGACAGA AGACAAGACA 
ACCGCTGCTG AAGATCCTTT TGCCAATTTA AGCAAAAAGG AGAAAAAGAA GAAAAAAAAA 
ATGCTGGAAT ATGAGAAACA AGTGGCTACT ATGAAGAAGA TAACTGCTGC AGAAAATGAC 
TTCTCTGTGT CACAAGCAGA ACTGTCATCA CGACAAGCCA TGCTGGAAAA TGCTTCAGAT 
ATAAAGATAG AAAAATTCAG CATTTCTGCC CACGGGAAGG AGTTGTTTGT TAATGCCGAT 
TTATATGTGG TAGCAGGTCG GCGCTATGGA TTGGTTGGGC CTAATGGGAA AGGCAAAACC 
ACTCTCCTAA AACACATAGC AAACAAGGCA CTGAACATAC CCCCAAACAT TGATGTCTTG 
CTGTGTGAGC AAGAGGTTGT TGCTGATGAC ACACCTGCAG TCCAGGCTGT GCTTAAAGCT 
GATAAGAAGC GACTAAAGTT GCTAGAGGAG GAGAGAAAAC TGCAAACCCG ACTTGAAAAG 
GGGGATGACA ATGCTGCCGA AAGATTAGAA AAGGTGTATG AGGAGTTAAG AGCTATGGGG 
GCTGCATCTG CCGAGGCCAA GGCGCGCAGG ATTCTGGCTG GTCTTGGCTT CACGCCTGAA 
ATGCAGGACA GAGAAACCCG TCGATTTAGT GGAGGTTGGA GAATGAGAGT CTCTTTGGCC 
AGAGCTCTCT TCATGGAGCC AACTCTACTG ATGCTGGATG AACCTACCAA TCACTTGGAT 
CTCAATGCTG TTATTTGGCT TAACAATTAC CTTCAGGGCT GGAAGAAAAC TCTTTTAATT 
GTGTCCCACG ATCAAGGGTT CTTAGATGAT GTCTGTACGG ATATAATGCA CCTGGATTCA 
CAGAAGCTGT ATTATTATCG AGGCAACTAC ATGACATTTA AGAAGATGTA CCAGCAAAAG 
CAGAAGGAAC TGCAGAAACA GTATGACAAG CAAGAGAAAA AACTCAAAGA CTTAAAAGCA 
GGAGGAAAGT CAGCTAAACA AGCGGAGAAG CAGACAAAAG AGGCACTAAC TCGCAAGCAG 
CAGAAGTGTC AGAAGAAGAA CAATGACAAG GAAGACAATG ATCCTGTTGA ATTACTAAAG 
AGGCCCAAGG AATATACTGT GAAATTCACT TTTCCAAATC CACCTCCACT CAGCCCTCCA 
ATTTTGGGTC TTCATGGTGT GGATTTTTGT TATTCTGGAC AAAAGCCACT GTTCAAAAAC 
TTGGATTTTG GCATAGACAT GGACTCTAGA GTGTGTATTG TGGGCCCCAA TGGAGTAGGG 
AAGAGTACAC TGCTCTTGCT GCTGACTGGA AAACTTACTC CTACCAAAGG AGAAATGAGA 
AAAAATCACA GACTGAAAAT AGGATTCTTT AACCAGCAAT ATGCAGATCA GCTAAACATG 
GAGGAGACAG CTACAGAATA TCTACAACGC AATTTCAATC TTCCATATCA GGATGCACGC 
AAATGTCTGG GAAGGTTTGG TCTAGAAAGC CATGCACATA CCATCCAGAT CTGCAAGCTG 
TCAGGAGGGC AGAAAGCTAG AGTGGTTTTT GCTGAGCTGT GTTGCAGGGA ACCTGACGTA 
TTAATTTTAG ATGAACCCAC CAATAACCTA GATATAGAGT CAATAGATGC TCTTGCGGAA 
GCGGTGAATG AATATAAAGG AGCTGTTATC ACAGTGAGCC ATGACGCCCG GCTTATTACA 
GAGACGAACT GTCACTTGTG GGTGGTAGAG GACCAGACTG TAAATCAAAT AGAGGGTGAT 
TTTGATGACT ATAAGAGAGA GGTCTTGGAG AGCCTGGGAG AAGTGATAGT GAACAAACCA 
CGGGAGAGGG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939514.2
CDS	120..2774
Translation	MPRGGKHEVGEEEEEEEVKGKETEKVVKKGKKDKKGKKSFFEELSADNKQAQEEEAAKEREKEKELQQQQQKKREKKKKDRRKQKGDEDDDDEEKKEREIAERLKKLSVQASDEDEEPDVKPAPKAGKKQGSNIFAALVEDQSDDEEEEEQVSNQVDEKKQSKGKKNVKGKHKAPEERPESEEDIPKEKSKGKKPVPSAASVNEEDEKKTEKGNKKSKPSQKPVSKAASVESDEEKKPEKGSKKTKPSQNRFGALDIEEDHVSGGSKEGSGDEGETEDKTTAAEDPFANLSKKEKKKKKKMLEYEKQVATMKKITAAENDFSVSQAELSSRQAMLENASDIKIEKFSISAHGKELFVNADLYVVAGRRYGLVGPNGKGKTTLLKHIANKALNIPPNIDVLLCEQEVVADDTPAVQAVLKADKKRLKLLEEERKLQTRLEKGDDNAAERLEKVYEELRAMGAASAEAKARRILAGLGFTPEMQDRETRRFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWKKTLLIVSHDQGFLDDVCTDIMHLDSQKLYYYRGNYMTFKKMYQQKQKELQKQYDKQEKKLKDLKAGGKSAKQAEKQTKEALTRKQQKCQKKNNDKEDNDPVELLKRPKEYTVKFTFPNPPPLSPPILGLHGVDFCYSGQKPLFKNLDFGIDMDSRVCIVGPNGVGKSTLLLLLTGKLTPTKGEMRKNHRLKIGFFNQQYADQLNMEETATEYLQRNFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELCCREPDVLILDEPTNNLDIESIDALAEAVNEYKGAVITVSHDARLITETNCHLWVVEDQTVNQIEGDFDDYKREVLESLGEVIVNKPRERD

Target ORF information:

RefSeq Version	XM_002939468.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ATP binding cassette subfamily F member 1 (abcf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939468.5

ORF Insert Sequence:

ATGCCTCGCG GAGGAAAGCA TGAGGTTGGA GAAGAAGAGG AGGAGGAGGA GGTGAAAGGG 
AAGGAAACAG AAAAAGTGGT GAAGAAAGGA AAAAAGGACA AGAAAGGGAA AAAATCGTTT 
TTTGAGGAGC TGTCTGCTGA TAACAAGCAG GCACAGGAAG AGGAGGCTGC GAAGGAACGG 
GAAAAGGAAA AGGAGTTGCA ACAGCAACAG CAAAAAAAGA GAGAAAAAAA GAAAAAAGAT 
CGTCGGAAGC AAAAAGGAGA CGAAGATGAT GATGATGAAG AAAAGAAAGA AAGAGAAATT 
GCAGAGAGGC TAAAAAAGCT GTCAGTGCAA GCAAGTGATG AAGATGAAGA ACCAGATGTA 
AAACCTGCAC CCAAAGCTGG GAAGAAGCAG GGGAGTAATA TCTTTGCAGC CCTAGTAGAG 
GATCAAAGTG ATGACGAAGA AGAAGAAGAG CAGGTATCCA ACCAGGTGGA TGAGAAAAAA 
CAAAGTAAAG GGAAGAAAAA TGTGAAAGGA AAACATAAGG CTCCTGAAGA AAGACCAGAA 
AGTGAGGAGG ATATTCCTAA GGAGAAATCA AAGGGCAAAA AACCTGTCCC CAGTGCTGCT 
TCTGTTAATG AAGAAGATGA GAAAAAAACT GAGAAGGGGA ATAAGAAATC AAAACCAAGT 
CAGAAACCTG TCTCCAAGGC TGCATCTGTT GAGTCTGATG AAGAGAAAAA ACCTGAGAAG 
GGGAGTAAAA AAACAAAACC AAGCCAGAAT CGGTTTGGTG CCTTAGACAT AGAGGAAGAT 
CATGTCAGTG GAGGATCAAA AGAAGGGTCA GGTGATGAGG GTGAGACAGA AGACAAGACA 
ACCGCTGCTG AAGATCCTTT TGCCAATTTA AGCAAAAAGG AGAAAAAGAA GAAAAAAAAA 
ATGCTGGAAT ATGAGAAACA AGTGGCTACT ATGAAGAAGA TAACTGCTGC AGAAAATGAC 
TTCTCTGTGT CACAAGCAGA ACTGTCATCA CGACAAGCCA TGCTGGAAAA TGCTTCAGAT 
ATAAAGATAG AAAAATTCAG CATTTCTGCC CACGGGAAGG AGTTGTTTGT TAATGCCGAT 
TTATATGTGG TAGCAGGTCG GCGCTATGGA TTGGTTGGGC CTAATGGGAA AGGCAAAACC 
ACTCTCCTAA AACACATAGC AAACAAGGCA CTGAACATAC CCCCAAACAT TGATGTCTTG 
CTGTGTGAGC AAGAGGTTGT TGCTGATGAC ACACCTGCAG TCCAGGCTGT GCTTAAAGCT 
GATAAGAAGC GACTAAAGTT GCTAGAGGAG GAGAGAAAAC TGCAAACCCG ACTTGAAAAG 
GGGGATGACA ATGCTGCCGA AAGATTAGAA AAGGTGTATG AGGAGTTAAG AGCTATGGGG 
GCTGCATCTG CCGAGGCCAA GGCGCGCAGG ATTCTGGCTG GTCTTGGCTT CACGCCTGAA 
ATGCAGGACA GAGAAACCCG TCGATTTAGT GGAGGTTGGA GAATGAGAGT CTCTTTGGCC 
AGAGCTCTCT TCATGGAGCC AACTCTACTG ATGCTGGATG AACCTACCAA TCACTTGGAT 
CTCAATGCTG TTATTTGGCT TAACAATTAC CTTCAGGGCT GGAAGAAAAC TCTTTTAATT 
GTGTCCCACG ATCAAGGGTT CTTAGATGAT GTCTGTACGG ATATAATGCA CCTGGATTCA 
CAGAAGCTGT ATTATTATCG AGGCAACTAC ATGACATTTA AGAAGATGTA CCAGCAAAAG 
CAGAAGGAAC TGCAGAAACA GTATGACAAG CAAGAGAAAA AACTCAAAGA CTTAAAAGCA 
GGAGGAAAGT CAGCTAAACA AGCGGAGAAG CAGACAAAAG AGGCACTAAC TCGCAAGCAG 
CAGAAGTGTC AGAAGAAGAA CAATGACAAG GAAGACAATG ATCCTGTTGA ATTACTAAAG 
AGGCCCAAGG AATATACTGT GAAATTCACT TTTCCAAATC CACCTCCACT CAGCCCTCCA 
ATTTTGGGTC TTCATGGTGT GGATTTTTGT TATTCTGGAC AAAAGCCACT GTTCAAAAAC 
TTGGATTTTG GCATAGACAT GGACTCTAGA GTGTGTATTG TGGGCCCCAA TGGAGTAGGG 
AAGAGTACAC TGCTCTTGCT GCTGACTGGA AAACTTACTC CTACCAAAGG AGAAATGAGA 
AAAAATCACA GACTGAAAAT AGGATTCTTT AACCAGCAAT ATGCAGATCA GCTAAACATG 
GAGGAGACAG CTACAGAATA TCTACAACGC AATTTCAATC TTCCATATCA GGATGCACGC 
AAATGTCTGG GAAGGTTTGG TCTAGAAAGC CATGCACATA CCATCCAGAT CTGCAAGCTG 
TCAGGAGGGC AGAAAGCTAG AGTGGTTTTT GCTGAGCTGT GTTGCAGGGA ACCTGACGTA 
TTAATTTTAG ATGAACCCAC CAATAACCTA GATATAGAGT CAATAGATGC TCTTGCGGAA 
GCGGTGAATG AATATAAAGG AGCTGTTATC ACAGTGAGCC ATGACGCCCG GCTTATTACA 
GAGACGAACT GTCACTTGTG GGTGGTAGAG GACCAGACTG TAAATCAAAT AGAGGGTGAT 
TTTGATGACT ATAAGAGAGA GGTCTTGGAG AGCCTGGGAG AAGTGATAGT GAACAAACCA 
CGGGAGAGGG ACTAA