kif1b gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012966952.2	XP_012822406.1	kinesin-like protein KIF1B isoform X3
XM_012966953.2	XP_012822407.1	kinesin-like protein KIF1B isoform X4
XM_031905974.1	XP_031761834.1	kinesin-like protein KIF1B isoform X2
XM_031905975.1	XP_031761835.1	kinesin-like protein KIF1B isoform X3
XM_012966961.3	XP_012822415.1	kinesin-like protein KIF1B isoform X7
XM_004916028.4	XP_004916085.1	kinesin-like protein KIF1B isoform X10
XM_012966956.2	XP_012822410.1	kinesin-like protein KIF1B isoform X7
XM_012966960.2	XP_012822414.1	kinesin-like protein KIF1B isoform X11
XM_004916028.3	XP_004916085.1	kinesin-like protein KIF1B isoform X12
XM_012966961.2	XP_012822415.1	kinesin-like protein KIF1B isoform X13
XM_018095819.1	XP_017951308.1	kinesin-like protein KIF1B isoform X14
XM_031905977.1	XP_031761837.1	kinesin-like protein KIF1B isoform X8
XM_012966950.2	XP_012822404.1	kinesin-like protein KIF1B isoform X1
XM_012966951.2	XP_012822405.1	kinesin-like protein KIF1B isoform X2
XM_012966954.2	XP_012822408.1	kinesin-like protein KIF1B isoform X5
XM_012966952.3	XP_012822406.1	kinesin-like protein KIF1B isoform X1
XM_031905976.1	XP_031761836.1	kinesin-like protein KIF1B isoform X4
XM_012966956.3	XP_012822410.1	kinesin-like protein KIF1B isoform X5
XM_018095819.2	XP_017951308.1	kinesin-like protein KIF1B isoform X6
XM_031905978.1	XP_031761838.1	kinesin-like protein KIF1B isoform X9
XM_012966955.2	XP_012822409.1	kinesin-like protein KIF1B isoform X6
XM_012966957.2	XP_012822411.1	kinesin-like protein KIF1B isoform X8
XM_012966958.2	XP_012822412.1	kinesin-like protein KIF1B isoform X9
XM_012966959.2	XP_012822413.1	kinesin-like protein KIF1B isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa62412	XM_031905978.1 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa62413	XM_004916028.4 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa12999	XM_004916028.3 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa45738	XM_018095819.1 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa62404	XM_012966952.3 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62405	XM_031905974.1 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62406	XM_031905975.1 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62407	XM_031905976.1 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62408	XM_012966956.3 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62409	XM_018095819.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62410	XM_012966961.3 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa62411	XM_031905977.1 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa32932	XM_012966950.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32933	XM_012966951.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32934	XM_012966952.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32935	XM_012966953.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32936	XM_012966954.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32937	XM_012966955.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32938	XM_012966956.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32939	XM_012966957.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32940	XM_012966958.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32941	XM_012966959.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa32942	XM_012966960.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa32943	XM_012966961.2 Latest version!	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

kif1b ( XM_031905978.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761838.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa62412
Clone ID Related Accession (Same CDS sequence)	XM_031905978.1
Accession Version	XM_031905978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGAT ATGCAGATCG TGCTAAACAG ATTAAGTGCA ATGCTGTTAT CAATGAAGAT 
CCCAATGCCA AACTTGTGCG TGAGCTAAAA GATGAAGTAA CAAGGCTTAA AGACCTCTTG 
CGTGCTCAGG GATTGGGCGA TATCCTTGAT ACTCTTCCTT CTGTCGGGTG CTTTCCTATC 
TCAGACATGA ATGATAATGA GAACCTTAAG AATAAATACT TATTAGCCAA TGAAAACCAA 
CGACCTGGCA ATTTCTCCAC AGCCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CAGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCCACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761838.1
CDS	81..4484
Translation	MRRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_031905978.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905978.1

ORF Insert Sequence:

ATGCGCAGAT ATGCAGATCG TGCTAAACAG ATTAAGTGCA ATGCTGTTAT CAATGAAGAT 
CCCAATGCCA AACTTGTGCG TGAGCTAAAA GATGAAGTAA CAAGGCTTAA AGACCTCTTG 
CGTGCTCAGG GATTGGGCGA TATCCTTGAT ACTCTTCCTT CTGTCGGGTG CTTTCCTATC 
TCAGACATGA ATGATAATGA GAACCTTAAG AATAAATACT TATTAGCCAA TGAAAACCAA 
CGACCTGGCA ATTTCTCCAC AGCCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CAGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCCACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_004916028.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916085.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X10, mRNA.

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CloneID	OXa62413
Clone ID Related Accession (Same CDS sequence)	XM_004916028.4
Accession Version	XM_004916028.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916028.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAT 
GCGGATTCAG ATAGTGGAGA TGACTCAGAG AAACGATCTT GCGAAGAAAG CTGGCGCTTA 
ATTACCTCTC TTCGAGAGAA GCTTCCACCT AGCAAAGTAC AAAGCATTGT TAAGAAATGT 
GGTCTTCCCA GCAGTGGTAA GAAAAGGGAG CCAAACAAAA TGTATCAGGT TCCCCATCGA 
CGAAGGCTTA GCAAGGATTC CAAGTGGGTG ACTATCTCTG ACCTAAAAAT ACAAGCTGTG 
AAAGAAATAT GTTATGAAGT GGCATTAAAT GACTTTCAGC ACAGCCGTCA GGAAATTGAA 
GCCCTTGCAA TTGTGAAAAT GAAAGAACTT TGTGCCATGT ATTGTAAAAA AGATCCTAAT 
GAACGAGACT CATGGCGAGC TGTAGCTCGG GATGTTTGGG ATACAGTGGG TGTTGGGGAT 
GAAAAAATTG AGGATTTAGT TACAGTTGGG AAGGCTTCAA CAGATGTGGA TGACCTAAAG 
GTTCATGTAG ACAAACTTGA AGACATATTA CAGGAGGTAA AGAACCAAAA TAATATGAAA 
GATGAGGAAA TTCGAGCCCT AAGGAACAAA ATGGTAAAAA TGGAGAAAGT ACTGCCACTG 
ATTGGATCAC ATGGAGAGCG AAAGATTCAG GATCTTTGCA TTGAAGGTGC AAAGACAATA 
ATAAAGAATG ACAAATTCAA ACCCATTGAG CCTGAAACAG AACAAGTTAA TAGTCCTGTG 
GAAAATGACT CAATGTTGTG CCGAGGACGT TCACACTGGA TGAGACAGGA GCAAATTAAA 
TTCAAGAACC TACAGCAGCA GGAAATCACC AAGCAGTTGC GTCGCAATAA TGGTCCTAAC 
CGATTCATCC CACCTGAAAA TCGTAAGCCT CGTTTCCCAT TTAAGAGCAA CCCCAGCCAC 
AGAAGTTCTT GGAGTCCTGG GACTCATATT ATTATAACTG ATGAGAAGGT GATTGAGGTA 
AAAGTTCCCA AAGAAGCTTA TATAAAAGAA CAGCCAGAGG AAATTGAACC AGAGAAACAA 
ATCTGGAGTC CTTATAGTCA TTTTCGTAAT CAACAATGGA TACAGCAGCA ACAGGAATAC 
AAGGGTTACA ACAATAGCCC AGAATCCCCG CCTACACGTT GGAGAAGCAA CTCCTTAAAT 
GGGCACCAAA AGGGACTTCG GCGTCAAGCT TCGGGTTCTT CAGAGTCCCT GCAGTCATGT 
GATTCTCAAC CAAATGACTT GCAGAATTTC AGCTATCGGC AATCTGGGCA TAAGCAAGGA 
CAGCAAAATT GGAGCAATCA TCAGAGCAGT AACAATGATG GTTATTCCAG CAATTACCAT 
AAGCAGGGTG ACCGGCCTCC TCACTACAAC CAGTTTGTAA CTCCACCACG CATGAGGCGC 
CAACATTCTG CTCCTAATCT CAAATCGAGT AGAGAAACCA CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916085.1
CDS	318..3725
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSEKRSCEESWRLITSLREKLPPSKVQSIVKKCGLPSSGKKREPNKMYQVPHRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFQHSRQEIEALAIVKMKELCAMYCKKDPNERDSWRAVARDVWDTVGVGDEKIEDLVTVGKASTDVDDLKVHVDKLEDILQEVKNQNNMKDEEIRALRNKMVKMEKVLPLIGSHGERKIQDLCIEGAKTIIKNDKFKPIEPETEQVNSPVENDSMLCRGRSHWMRQEQIKFKNLQQQEITKQLRRNNGPNRFIPPENRKPRFPFKSNPSHRSSWSPGTHIIITDEKVIEVKVPKEAYIKEQPEEIEPEKQIWSPYSHFRNQQWIQQQQEYKGYNNSPESPPTRWRSNSLNGHQKGLRRQASGSSESLQSCDSQPNDLQNFSYRQSGHKQGQQNWSNHQSSNNDGYSSNYHKQGDRPPHYNQFVTPPRMRRQHSAPNLKSSRETTV

Target ORF information:

RefSeq Version	XM_004916028.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916028.4

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAT 
GCGGATTCAG ATAGTGGAGA TGACTCAGAG AAACGATCTT GCGAAGAAAG CTGGCGCTTA 
ATTACCTCTC TTCGAGAGAA GCTTCCACCT AGCAAAGTAC AAAGCATTGT TAAGAAATGT 
GGTCTTCCCA GCAGTGGTAA GAAAAGGGAG CCAAACAAAA TGTATCAGGT TCCCCATCGA 
CGAAGGCTTA GCAAGGATTC CAAGTGGGTG ACTATCTCTG ACCTAAAAAT ACAAGCTGTG 
AAAGAAATAT GTTATGAAGT GGCATTAAAT GACTTTCAGC ACAGCCGTCA GGAAATTGAA 
GCCCTTGCAA TTGTGAAAAT GAAAGAACTT TGTGCCATGT ATTGTAAAAA AGATCCTAAT 
GAACGAGACT CATGGCGAGC TGTAGCTCGG GATGTTTGGG ATACAGTGGG TGTTGGGGAT 
GAAAAAATTG AGGATTTAGT TACAGTTGGG AAGGCTTCAA CAGATGTGGA TGACCTAAAG 
GTTCATGTAG ACAAACTTGA AGACATATTA CAGGAGGTAA AGAACCAAAA TAATATGAAA 
GATGAGGAAA TTCGAGCCCT AAGGAACAAA ATGGTAAAAA TGGAGAAAGT ACTGCCACTG 
ATTGGATCAC ATGGAGAGCG AAAGATTCAG GATCTTTGCA TTGAAGGTGC AAAGACAATA 
ATAAAGAATG ACAAATTCAA ACCCATTGAG CCTGAAACAG AACAAGTTAA TAGTCCTGTG 
GAAAATGACT CAATGTTGTG CCGAGGACGT TCACACTGGA TGAGACAGGA GCAAATTAAA 
TTCAAGAACC TACAGCAGCA GGAAATCACC AAGCAGTTGC GTCGCAATAA TGGTCCTAAC 
CGATTCATCC CACCTGAAAA TCGTAAGCCT CGTTTCCCAT TTAAGAGCAA CCCCAGCCAC 
AGAAGTTCTT GGAGTCCTGG GACTCATATT ATTATAACTG ATGAGAAGGT GATTGAGGTA 
AAAGTTCCCA AAGAAGCTTA TATAAAAGAA CAGCCAGAGG AAATTGAACC AGAGAAACAA 
ATCTGGAGTC CTTATAGTCA TTTTCGTAAT CAACAATGGA TACAGCAGCA ACAGGAATAC 
AAGGGTTACA ACAATAGCCC AGAATCCCCG CCTACACGTT GGAGAAGCAA CTCCTTAAAT 
GGGCACCAAA AGGGACTTCG GCGTCAAGCT TCGGGTTCTT CAGAGTCCCT GCAGTCATGT 
GATTCTCAAC CAAATGACTT GCAGAATTTC AGCTATCGGC AATCTGGGCA TAAGCAAGGA 
CAGCAAAATT GGAGCAATCA TCAGAGCAGT AACAATGATG GTTATTCCAG CAATTACCAT 
AAGCAGGGTG ACCGGCCTCC TCACTACAAC CAGTTTGTAA CTCCACCACG CATGAGGCGC 
CAACATTCTG CTCCTAATCT CAAATCGAGT AGAGAAACCA CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_004916028.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916085.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa12999
Clone ID Related Accession (Same CDS sequence)	XM_004916028.3
Accession Version	XM_004916028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAT 
GCGGATTCAG ATAGTGGAGA TGACTCAGAG AAACGATCTT GCGAAGAAAG CTGGCGCTTA 
ATTACCTCTC TTCGAGAGAA GCTTCCACCT AGCAAAGTAC AAAGCATTGT TAAGAAATGT 
GGTCTTCCCA GCAGTGGTAA GAAAAGGGAG CCAAACAAAA TGTATCAGGT TCCCCATCGA 
CGAAGGCTTA GCAAGGATTC CAAGTGGGTG ACTATCTCTG ACCTAAAAAT ACAAGCTGTG 
AAAGAAATAT GTTATGAAGT GGCATTAAAT GACTTTCAGC ACAGCCGTCA GGAAATTGAA 
GCCCTTGCAA TTGTGAAAAT GAAAGAACTT TGTGCCATGT ATTGTAAAAA AGATCCTAAT 
GAACGAGACT CATGGCGAGC TGTAGCTCGG GATGTTTGGG ATACAGTGGG TGTTGGGGAT 
GAAAAAATTG AGGATTTAGT TACAGTTGGG AAGGCTTCAA CAGATGTGGA TGACCTAAAG 
GTTCATGTAG ACAAACTTGA AGACATATTA CAGGAGGTAA AGAACCAAAA TAATATGAAA 
GATGAGGAAA TTCGAGCCCT AAGGAACAAA ATGGTAAAAA TGGAGAAAGT ACTGCCACTG 
ATTGGATCAC ATGGAGAGCG AAAGATTCAG GATCTTTGCA TTGAAGGTGC AAAGACAATA 
ATAAAGAATG ACAAATTCAA ACCCATTGAG CCTGAAACAG AACAAGTTAA TAGTCCTGTG 
GAAAATGACT CAATGTTGTG CCGAGGACGT TCACACTGGA TGAGACAGGA GCAAATTAAA 
TTCAAGAACC TACAGCAGCA GGAAATCACC AAGCAGTTGC GTCGCAATAA TGGTCCTAAC 
CGATTCATCC CACCTGAAAA TCGTAAGCCT CGTTTCCCAT TTAAGAGCAA CCCCAGCCAC 
AGAAGTTCTT GGAGTCCTGG GACTCATATT ATTATAACTG ATGAGAAGGT GATTGAGGTA 
AAAGTTCCCA AAGAAGCTTA TATAAAAGAA CAGCCAGAGG AAATTGAACC AGAGAAACAA 
ATCTGGAGTC CTTATAGTCA TTTTCGTAAT CAACAATGGA TACAGCAGCA ACAGGAATAC 
AAGGGTTACA ACAATAGCCC AGAATCCCCG CCTACACGTT GGAGAAGCAA CTCCTTAAAT 
GGGCACCAAA AGGGACTTCG GCGTCAAGCT TCGGGTTCTT CAGAGTCCCT GCAGTCATGT 
GATTCTCAAC CAAATGACTT GCAGAATTTC AGCTATCGGC AATCTGGGCA TAAGCAAGGA 
CAGCAAAATT GGAGCAATCA TCAGAGCAGT AACAATGATG GTTATTCCAG CAATTACCAT 
AAGCAGGGTG ACCGGCCTCC TCACTACAAC CAGTTTGTAA CTCCACCACG CATGAGGCGC 
CAACATTCTG CTCCTAATCT CAAATCGAGT AGAGAAACCA CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916085.1
CDS	331..3738
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSEKRSCEESWRLITSLREKLPPSKVQSIVKKCGLPSSGKKREPNKMYQVPHRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFQHSRQEIEALAIVKMKELCAMYCKKDPNERDSWRAVARDVWDTVGVGDEKIEDLVTVGKASTDVDDLKVHVDKLEDILQEVKNQNNMKDEEIRALRNKMVKMEKVLPLIGSHGERKIQDLCIEGAKTIIKNDKFKPIEPETEQVNSPVENDSMLCRGRSHWMRQEQIKFKNLQQQEITKQLRRNNGPNRFIPPENRKPRFPFKSNPSHRSSWSPGTHIIITDEKVIEVKVPKEAYIKEQPEEIEPEKQIWSPYSHFRNQQWIQQQQEYKGYNNSPESPPTRWRSNSLNGHQKGLRRQASGSSESLQSCDSQPNDLQNFSYRQSGHKQGQQNWSNHQSSNNDGYSSNYHKQGDRPPHYNQFVTPPRMRRQHSAPNLKSSRETTV

Target ORF information:

RefSeq Version	XM_004916028.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916028.3

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAT 
GCGGATTCAG ATAGTGGAGA TGACTCAGAG AAACGATCTT GCGAAGAAAG CTGGCGCTTA 
ATTACCTCTC TTCGAGAGAA GCTTCCACCT AGCAAAGTAC AAAGCATTGT TAAGAAATGT 
GGTCTTCCCA GCAGTGGTAA GAAAAGGGAG CCAAACAAAA TGTATCAGGT TCCCCATCGA 
CGAAGGCTTA GCAAGGATTC CAAGTGGGTG ACTATCTCTG ACCTAAAAAT ACAAGCTGTG 
AAAGAAATAT GTTATGAAGT GGCATTAAAT GACTTTCAGC ACAGCCGTCA GGAAATTGAA 
GCCCTTGCAA TTGTGAAAAT GAAAGAACTT TGTGCCATGT ATTGTAAAAA AGATCCTAAT 
GAACGAGACT CATGGCGAGC TGTAGCTCGG GATGTTTGGG ATACAGTGGG TGTTGGGGAT 
GAAAAAATTG AGGATTTAGT TACAGTTGGG AAGGCTTCAA CAGATGTGGA TGACCTAAAG 
GTTCATGTAG ACAAACTTGA AGACATATTA CAGGAGGTAA AGAACCAAAA TAATATGAAA 
GATGAGGAAA TTCGAGCCCT AAGGAACAAA ATGGTAAAAA TGGAGAAAGT ACTGCCACTG 
ATTGGATCAC ATGGAGAGCG AAAGATTCAG GATCTTTGCA TTGAAGGTGC AAAGACAATA 
ATAAAGAATG ACAAATTCAA ACCCATTGAG CCTGAAACAG AACAAGTTAA TAGTCCTGTG 
GAAAATGACT CAATGTTGTG CCGAGGACGT TCACACTGGA TGAGACAGGA GCAAATTAAA 
TTCAAGAACC TACAGCAGCA GGAAATCACC AAGCAGTTGC GTCGCAATAA TGGTCCTAAC 
CGATTCATCC CACCTGAAAA TCGTAAGCCT CGTTTCCCAT TTAAGAGCAA CCCCAGCCAC 
AGAAGTTCTT GGAGTCCTGG GACTCATATT ATTATAACTG ATGAGAAGGT GATTGAGGTA 
AAAGTTCCCA AAGAAGCTTA TATAAAAGAA CAGCCAGAGG AAATTGAACC AGAGAAACAA 
ATCTGGAGTC CTTATAGTCA TTTTCGTAAT CAACAATGGA TACAGCAGCA ACAGGAATAC 
AAGGGTTACA ACAATAGCCC AGAATCCCCG CCTACACGTT GGAGAAGCAA CTCCTTAAAT 
GGGCACCAAA AGGGACTTCG GCGTCAAGCT TCGGGTTCTT CAGAGTCCCT GCAGTCATGT 
GATTCTCAAC CAAATGACTT GCAGAATTTC AGCTATCGGC AATCTGGGCA TAAGCAAGGA 
CAGCAAAATT GGAGCAATCA TCAGAGCAGT AACAATGATG GTTATTCCAG CAATTACCAT 
AAGCAGGGTG ACCGGCCTCC TCACTACAAC CAGTTTGTAA CTCCACCACG CATGAGGCGC 
CAACATTCTG CTCCTAATCT CAAATCGAGT AGAGAAACCA CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_018095819.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951308.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X14, mRNA.

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CloneID	OXa45738
Clone ID Related Accession (Same CDS sequence)	XM_018095819.1
Accession Version	XM_018095819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951308.1
CDS	358..5541
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_018095819.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095819.1

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966952.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822406.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X1, mRNA.

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CloneID	OXa62404
Clone ID Related Accession (Same CDS sequence)	XM_012966952.3
Accession Version	XM_012966952.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CACCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC AGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCCACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822406.1
CDS	331..5763
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966952.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966952.3

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CACCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC AGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCCACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_031905974.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761834.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X2, mRNA.

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CloneID	OXa62405
Clone ID Related Accession (Same CDS sequence)	XM_031905974.1
Accession Version	XM_031905974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACTCTTCCT TCTGTCGGGT GCTTTCCTAT CTCAGACATG 
AATGATAATG AGAACCTTAA GAATAAATAC TTATTAGCCA ATGAAAACCA ACGACCTGGC 
AATTTCTCCA CAGCCTCCAT CAGTTCTCTA GCATCCTCAC CATCCTCCTG TTCACTTAGT 
AGTCAGGTGG GATTGACATC TGTTACCAGC ATCCAGGAAA GAATCATGTC TACCCCAGGA 
GGAGAGGAAG CTATAGAGCG ACTTAAAGAG TCTGAAAAAA TAATTGCAGA GCTAAATGAG 
ACATGGGAAG AGAAGCTGCG AAAAACAGAG GCTATTCGAA TGGACAGGGA GGCTTTACTT 
GCAGAAATGG GAGTGGCTAT TCGTGAAGAT GGTGGAACCT TGGGGGTTTT CTCACCCAAA 
AAGACTCCCC ATCTTGTCAA TCTTAATGAA GATCCACTAA TGTCTGAATG CCTTCTATAC 
TACATTAAAG ATGGACTTAC AAGGGTCGGT CAGGCAGATG CTGATAGTCG CCAGGATATT 
GTGCTAAGTG GAGCTCATAT TAAAGAGGAA CACTGCATAT TCCGTAGTGA AAGGAATTCA 
AATGGGGAAG TAATAGTAAC CTTGGAGCCC GGTGACAGGT CAGAAACATA TGTCAATGGA 
AAACGAGTTG TGCATCCAGT GGAGTTAAGA TCTGGAAATC GAATAATAAT GGGAAAAAAC 
CATGTATTTC GTTTTAACCA CCCTGAGCAA GCCCGTGCTG AACGGGAGAA AACACCTTCT 
GCAGAAACAC CCTCAGAACC AGTGGACTGG ACATTTGCTC AGCGTGAGCT GTTGGAGAAA 
CAAGGCATCG ATATGAAGCA AGAAATGGAG AAAAGGCTTC AGGAGATGGA AATTTTGTAC 
AAGAAAGAAA AGGAAGAGGC AGATCTTCTT TTGGAGCAAC AAAGGCTGGA CTATGAGAGC 
AAACTGCAAG CTCTGCAAAA ACAAGTCGAG ACTCACTCAC TGGCAGCAGA GACCACAGAT 
GATGGGGAAG AAGAAGAGGA GGAAGTTCCA TGGACACAGC ATGAATATGA TGTAGCACTG 
TGGGCATTTA AAAAGTGGAG AAATCACCAG TTCACCTCGT TACGGGACTT GCTTTGGGGG 
AATGCCATGT ATCTGAAGGA AGCCAATGCA ATAAGTGTAG AGCTGAAGAA GAAGGTGCAG 
TTCCAATTTG TGCTGCTTAC AGATACTTTG TACTCCCCAC TGCCACCAGA GTTACTTCCT 
TCTGATGCTG ATAAAACCAC AGAAGACAGG ACTTTCCCTC GAACTGTGGT TGCAGTTGAG 
GTGCAAGATT TAAAAAATGG GGCGACTCAT TACTGGTCAC TGGAGAAACT GAGGTTAAGG 
TTAGAACAGA TGCGGGAGAT GTATGACCGT GCAGGAGAAA TGGCTTCCCA TGTGCAAGAT 
GAAAATGACA CAGTGATAAC TGGAAATGAC CCTTTTTATG ATCGCTTTCA TTGGTTCAAG 
CTAGTTGGGA GTTCTCCCAT ATTCCACGGC TGTGTGAATG AGCGGCTGGC AGACCGCACC 
CCGTCCCCTA CCTTCTCCAC TGCAGACTCT GATATCACAG AGTTAGCTGA TGAGCAGCAG 
GACCAGATGG AAGACTTTGA TGATGAGCCA TTTGTGGATG ACACTGGCTC TGATGTTGGT 
GCAGAAGAAG GATCCGATCT CTTCAGTGAT GGCCATGACC CGTTTTACGA CCGGTCCCCA 
TGGTTCATTT TAGTAGGAAG GGCTTTCGTC TATCTAAGCA ACCTGCTTTT CCCAGTTCCT 
CTTGTGCATC GAGCAGCTAT AGTAAGTGAG AAAGGAGAAG TACGTGGGTT TTTGCGTGTT 
GCTGTGCAGG CAATATCTGG TGATGAGGAA GCACCAGACT ATGGGTCCGG CATTAGACAA 
TCGGGAACAG CCAAGATTTC ATTTGATAAT GAATACATTA ATAAGAATGA AATCTCTTCT 
ATGGTTTTGA CTCGTTCCGG ACTGTCACTA GAAGAACTTC GCATTGTGGA AGGACAAGGA 
CAGAACTCTG ACATTACTTC ATTAGAAGAG GTCAATCATT TAGCTGATTT AGACCTAAAG 
TCTGGAAACC TGTTGGATGG AAAGATGATG ATGGAAGGAT TCTCAGAGGA TGTAGGAGAG 
CATCTGAAAA TTGGTAGCAT GTTCACTTTC CGTGTGACAG TGCTTCAGGC CAGTGGAATT 
CCTCCAGAAT ATGCTGATAT CTTCTGCCAA TTTAACTTTT TACATCGACA TGATGAAGCA 
TTTTCCACAG AGCCACTGAA GAACAGTGGC AGAGGAAATC CTCTTGGTTT CTATCATGTT 
CAGAATATTG CAGTGGAAAT TACAGAATCT TTTATAGAAT ACATTAAAAA CAACCCAATT 
GTTTTTGAAG TATTTGGCCA TTACCAGCAG CACCCTCTCC ACCTTCAAGG ACAAGATCTC 
AGCCCACCTC AGCAGTGCCG ACGTTACTTT CCTCCTCCAA TGCCACTTTC TAAACCAGTT 
CCAGCCACTA AATTAAATCC ACTAAGCAAG CAAAGTCTTG GACAGGTCAT CAGCAAGTAT 
GACTTGTTAG TCTGGTTTGA GATCAGTGAA CTGGAGCCTA CTGGAGAGTA CATTCCAGCT 
GTCGTAGATC ATTCTGGAGG ACTGCCCTGC CAGGGAACCT TTTTGCTGCA TCAGGGAATC 
CAGCGCAGAA TTACTGTCAC CATTGTCCAT GAGAAAGGGA ATGAGTTGCA CTGGAAGGAT 
GTGAGAGAAT TGGTTGTAGG ACGTGTTCGC AATAAAGCAG ATGAAGCTGC TGCAGATGCT 
GTTCTTTCAC TAAATATCAT CTCTGCAAAA TACCTCAGGT CTTCTCATAG CTCTGGCAGA 
ACTTTTTATC GCTTTGAGGC AGTATGGGAC AGTTCCTTGC ATAACTCCCT GCTTCTAAAC 
CGGGTAACTC CATATGGAGA GAAGATCTAT ATGACCCTAT CAGCGTATCT TGAGCTTGAT 
CACTGCATCC AGCCTGCAGT TATTACAAAA GATGTCTGTA TGATCTTTTA CTCTCGTGAT 
GCCAAAATAT CTCCTCCACG GTCTCTGCGC AGTCTATTTG GCAGTGGTTA CTCAAAATCC 
CCTGACTGTA ACAGAGTAAC TGGAATATAT GAGTTGAGTT TGTGCAAAAT GGCTGATACC 
GGCAGCCCTG GAATGCAACG AAGAAGAAGA AAGATCCTCG ATACGTCAGT GGCATATGTT 
CGTGGTGAAG AGAATCTGGC AGGATGGAGA CCTCGAGGCG ATAGTCTAAT CCTAGAGCAT 
CAATGGGAAC TTGAAAAGAT GGAGCAATTG CATGAGGTTG AGAAAACAAG GCACCTCCTT 
TTACTTAGAG ACAAACTAGG AGACAGTATC CCTAAGTCCC TAAGTGAATC TATCTCTCCA 
AGCTTGAGCA GTGGCACCCT AAGCACATCG ACCAGTATTT CATCGCAGAT ATCCACCACA 
ACCTTTGAAA GTGCCATCAC ACCTAGTGAG AGCAGTGGAT ATGACTCTAC TGATGTTGAG 
AGTCTTGTGG ATCGTGAGAA GGAGTTAGCA ACCAAGTGTT TGCGACTTTT GACTCACACA 
TTTAACCAAG AACTAACCCA GGTGTGTAAC AGTATCAGTG ACTGTAAGTT GTCTGATATC 
TCCCCAATTG GAAGGGATCC CTCGGTCTCC AGCTTTAGCA GTGCTACTCT CACTCCTTCA 
TCAACTTGCC CATCACTAGC AGATTCTCGA TGCAGCTCTG TGGATCAGAA GACCCCTGAA 
GCAAATTCAC GTGCCTCCAG CCCTTGCACT GAATTTGAAC AGTTCCATTT GGTACCAGCT 
GTGGAGCGGT CATTTTTGGC TCGTGCAGGA AAGAACGAGT TTCTAAATCT TGTACCAGAT 
ATTGAAGAGA TTCGTCCAGG ATCTGTGGTC TCAAAGAAAG GTTACTTGAA CTTCAAAGAA 
CCTCTGTCTC ATTCCTGGGT CAAGCATTTT GTGGTTGTGC GCCGTCCCTA TGTCTTCATC 
TACAACAGTG ACAAGGACCC AGTAGAGAGG GGCCTCATTA ACTTATCCAC TGCACAAGTG 
GAGTACAGTG AAGACCAGCA AGCCATGGTT AAGAGCTCAA ACATATTTGC TGTATGCACA 
ATGTACCGTG GGATCCTTCT GCAGGCCATT AATGATAAAG ATATGAATGA CTGGTTGTAT 
GCCTTTAATC CTCTGCTTGC TGGCACAATA AGGTCAAAGC TGGCAAGACG TCGTTCTCTT 
TCCTTGAAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761834.1
CDS	331..5745
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_031905974.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905974.1

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACTCTTCCT TCTGTCGGGT GCTTTCCTAT CTCAGACATG 
AATGATAATG AGAACCTTAA GAATAAATAC TTATTAGCCA ATGAAAACCA ACGACCTGGC 
AATTTCTCCA CAGCCTCCAT CAGTTCTCTA GCATCCTCAC CATCCTCCTG TTCACTTAGT 
AGTCAGGTGG GATTGACATC TGTTACCAGC ATCCAGGAAA GAATCATGTC TACCCCAGGA 
GGAGAGGAAG CTATAGAGCG ACTTAAAGAG TCTGAAAAAA TAATTGCAGA GCTAAATGAG 
ACATGGGAAG AGAAGCTGCG AAAAACAGAG GCTATTCGAA TGGACAGGGA GGCTTTACTT 
GCAGAAATGG GAGTGGCTAT TCGTGAAGAT GGTGGAACCT TGGGGGTTTT CTCACCCAAA 
AAGACTCCCC ATCTTGTCAA TCTTAATGAA GATCCACTAA TGTCTGAATG CCTTCTATAC 
TACATTAAAG ATGGACTTAC AAGGGTCGGT CAGGCAGATG CTGATAGTCG CCAGGATATT 
GTGCTAAGTG GAGCTCATAT TAAAGAGGAA CACTGCATAT TCCGTAGTGA AAGGAATTCA 
AATGGGGAAG TAATAGTAAC CTTGGAGCCC GGTGACAGGT CAGAAACATA TGTCAATGGA 
AAACGAGTTG TGCATCCAGT GGAGTTAAGA TCTGGAAATC GAATAATAAT GGGAAAAAAC 
CATGTATTTC GTTTTAACCA CCCTGAGCAA GCCCGTGCTG AACGGGAGAA AACACCTTCT 
GCAGAAACAC CCTCAGAACC AGTGGACTGG ACATTTGCTC AGCGTGAGCT GTTGGAGAAA 
CAAGGCATCG ATATGAAGCA AGAAATGGAG AAAAGGCTTC AGGAGATGGA AATTTTGTAC 
AAGAAAGAAA AGGAAGAGGC AGATCTTCTT TTGGAGCAAC AAAGGCTGGA CTATGAGAGC 
AAACTGCAAG CTCTGCAAAA ACAAGTCGAG ACTCACTCAC TGGCAGCAGA GACCACAGAT 
GATGGGGAAG AAGAAGAGGA GGAAGTTCCA TGGACACAGC ATGAATATGA TGTAGCACTG 
TGGGCATTTA AAAAGTGGAG AAATCACCAG TTCACCTCGT TACGGGACTT GCTTTGGGGG 
AATGCCATGT ATCTGAAGGA AGCCAATGCA ATAAGTGTAG AGCTGAAGAA GAAGGTGCAG 
TTCCAATTTG TGCTGCTTAC AGATACTTTG TACTCCCCAC TGCCACCAGA GTTACTTCCT 
TCTGATGCTG ATAAAACCAC AGAAGACAGG ACTTTCCCTC GAACTGTGGT TGCAGTTGAG 
GTGCAAGATT TAAAAAATGG GGCGACTCAT TACTGGTCAC TGGAGAAACT GAGGTTAAGG 
TTAGAACAGA TGCGGGAGAT GTATGACCGT GCAGGAGAAA TGGCTTCCCA TGTGCAAGAT 
GAAAATGACA CAGTGATAAC TGGAAATGAC CCTTTTTATG ATCGCTTTCA TTGGTTCAAG 
CTAGTTGGGA GTTCTCCCAT ATTCCACGGC TGTGTGAATG AGCGGCTGGC AGACCGCACC 
CCGTCCCCTA CCTTCTCCAC TGCAGACTCT GATATCACAG AGTTAGCTGA TGAGCAGCAG 
GACCAGATGG AAGACTTTGA TGATGAGCCA TTTGTGGATG ACACTGGCTC TGATGTTGGT 
GCAGAAGAAG GATCCGATCT CTTCAGTGAT GGCCATGACC CGTTTTACGA CCGGTCCCCA 
TGGTTCATTT TAGTAGGAAG GGCTTTCGTC TATCTAAGCA ACCTGCTTTT CCCAGTTCCT 
CTTGTGCATC GAGCAGCTAT AGTAAGTGAG AAAGGAGAAG TACGTGGGTT TTTGCGTGTT 
GCTGTGCAGG CAATATCTGG TGATGAGGAA GCACCAGACT ATGGGTCCGG CATTAGACAA 
TCGGGAACAG CCAAGATTTC ATTTGATAAT GAATACATTA ATAAGAATGA AATCTCTTCT 
ATGGTTTTGA CTCGTTCCGG ACTGTCACTA GAAGAACTTC GCATTGTGGA AGGACAAGGA 
CAGAACTCTG ACATTACTTC ATTAGAAGAG GTCAATCATT TAGCTGATTT AGACCTAAAG 
TCTGGAAACC TGTTGGATGG AAAGATGATG ATGGAAGGAT TCTCAGAGGA TGTAGGAGAG 
CATCTGAAAA TTGGTAGCAT GTTCACTTTC CGTGTGACAG TGCTTCAGGC CAGTGGAATT 
CCTCCAGAAT ATGCTGATAT CTTCTGCCAA TTTAACTTTT TACATCGACA TGATGAAGCA 
TTTTCCACAG AGCCACTGAA GAACAGTGGC AGAGGAAATC CTCTTGGTTT CTATCATGTT 
CAGAATATTG CAGTGGAAAT TACAGAATCT TTTATAGAAT ACATTAAAAA CAACCCAATT 
GTTTTTGAAG TATTTGGCCA TTACCAGCAG CACCCTCTCC ACCTTCAAGG ACAAGATCTC 
AGCCCACCTC AGCAGTGCCG ACGTTACTTT CCTCCTCCAA TGCCACTTTC TAAACCAGTT 
CCAGCCACTA AATTAAATCC ACTAAGCAAG CAAAGTCTTG GACAGGTCAT CAGCAAGTAT 
GACTTGTTAG TCTGGTTTGA GATCAGTGAA CTGGAGCCTA CTGGAGAGTA CATTCCAGCT 
GTCGTAGATC ATTCTGGAGG ACTGCCCTGC CAGGGAACCT TTTTGCTGCA TCAGGGAATC 
CAGCGCAGAA TTACTGTCAC CATTGTCCAT GAGAAAGGGA ATGAGTTGCA CTGGAAGGAT 
GTGAGAGAAT TGGTTGTAGG ACGTGTTCGC AATAAAGCAG ATGAAGCTGC TGCAGATGCT 
GTTCTTTCAC TAAATATCAT CTCTGCAAAA TACCTCAGGT CTTCTCATAG CTCTGGCAGA 
ACTTTTTATC GCTTTGAGGC AGTATGGGAC AGTTCCTTGC ATAACTCCCT GCTTCTAAAC 
CGGGTAACTC CATATGGAGA GAAGATCTAT ATGACCCTAT CAGCGTATCT TGAGCTTGAT 
CACTGCATCC AGCCTGCAGT TATTACAAAA GATGTCTGTA TGATCTTTTA CTCTCGTGAT 
GCCAAAATAT CTCCTCCACG GTCTCTGCGC AGTCTATTTG GCAGTGGTTA CTCAAAATCC 
CCTGACTGTA ACAGAGTAAC TGGAATATAT GAGTTGAGTT TGTGCAAAAT GGCTGATACC 
GGCAGCCCTG GAATGCAACG AAGAAGAAGA AAGATCCTCG ATACGTCAGT GGCATATGTT 
CGTGGTGAAG AGAATCTGGC AGGATGGAGA CCTCGAGGCG ATAGTCTAAT CCTAGAGCAT 
CAATGGGAAC TTGAAAAGAT GGAGCAATTG CATGAGGTTG AGAAAACAAG GCACCTCCTT 
TTACTTAGAG ACAAACTAGG AGACAGTATC CCTAAGTCCC TAAGTGAATC TATCTCTCCA 
AGCTTGAGCA GTGGCACCCT AAGCACATCG ACCAGTATTT CATCGCAGAT ATCCACCACA 
ACCTTTGAAA GTGCCATCAC ACCTAGTGAG AGCAGTGGAT ATGACTCTAC TGATGTTGAG 
AGTCTTGTGG ATCGTGAGAA GGAGTTAGCA ACCAAGTGTT TGCGACTTTT GACTCACACA 
TTTAACCAAG AACTAACCCA GGTGTGTAAC AGTATCAGTG ACTGTAAGTT GTCTGATATC 
TCCCCAATTG GAAGGGATCC CTCGGTCTCC AGCTTTAGCA GTGCTACTCT CACTCCTTCA 
TCAACTTGCC CATCACTAGC AGATTCTCGA TGCAGCTCTG TGGATCAGAA GACCCCTGAA 
GCAAATTCAC GTGCCTCCAG CCCTTGCACT GAATTTGAAC AGTTCCATTT GGTACCAGCT 
GTGGAGCGGT CATTTTTGGC TCGTGCAGGA AAGAACGAGT TTCTAAATCT TGTACCAGAT 
ATTGAAGAGA TTCGTCCAGG ATCTGTGGTC TCAAAGAAAG GTTACTTGAA CTTCAAAGAA 
CCTCTGTCTC ATTCCTGGGT CAAGCATTTT GTGGTTGTGC GCCGTCCCTA TGTCTTCATC 
TACAACAGTG ACAAGGACCC AGTAGAGAGG GGCCTCATTA ACTTATCCAC TGCACAAGTG 
GAGTACAGTG AAGACCAGCA AGCCATGGTT AAGAGCTCAA ACATATTTGC TGTATGCACA 
ATGTACCGTG GGATCCTTCT GCAGGCCATT AATGATAAAG ATATGAATGA CTGGTTGTAT 
GCCTTTAATC CTCTGCTTGC TGGCACAATA AGGTCAAAGC TGGCAAGACG TCGTTCTCTT 
TCCTTGAAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_031905975.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761835.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X3, mRNA.

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CloneID	OXa62406
Clone ID Related Accession (Same CDS sequence)	XM_031905975.1
Accession Version	XM_031905975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CACCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC AGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCCACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGT TGTCTGATAT CTCCCCAATT 
GGAAGGGATC CCTCGGTCTC CAGCTTTAGC AGTGCTACTC TCACTCCTTC ATCAACTTGC 
CCATCACTAG CAGATTCTCG ATGCAGCTCT GTGGATCAGA AGACCCCTGA AGCAAATTCA 
CGTGCCTCCA GCCCTTGCAC TGAATTTGAA CAGTTCCATT TGGTACCAGC TGTGGAGCGG 
TCATTTTTGG CTCGTGCAGG AAAGAACGAG TTTCTAAATC TTGTACCAGA TATTGAAGAG 
ATTCGTCCAG GATCTGTGGT CTCAAAGAAA GGTTACTTGA ACTTCAAAGA ACCTCTGTCT 
CATTCCTGGG TCAAGCATTT TGTGGTTGTG CGCCGTCCCT ATGTCTTCAT CTACAACAGT 
GACAAGGACC CAGTAGAGAG GGGCCTCATT AACTTATCCA CTGCACAAGT GGAGTACAGT 
GAAGACCAGC AAGCCATGGT TAAGAGCTCA AACATATTTG CTGTATGCAC AATGTACCGT 
GGGATCCTTC TGCAGGCCAT TAATGATAAA GATATGAATG ACTGGTTGTA TGCCTTTAAT 
CCTCTGCTTG CTGGCACAAT AAGGTCAAAG CTGGCAAGAC GTCGTTCTCT TTCCTTGAAG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761835.1
CDS	331..5736
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_031905975.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905975.1

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CACCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC AGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCCACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGT TGTCTGATAT CTCCCCAATT 
GGAAGGGATC CCTCGGTCTC CAGCTTTAGC AGTGCTACTC TCACTCCTTC ATCAACTTGC 
CCATCACTAG CAGATTCTCG ATGCAGCTCT GTGGATCAGA AGACCCCTGA AGCAAATTCA 
CGTGCCTCCA GCCCTTGCAC TGAATTTGAA CAGTTCCATT TGGTACCAGC TGTGGAGCGG 
TCATTTTTGG CTCGTGCAGG AAAGAACGAG TTTCTAAATC TTGTACCAGA TATTGAAGAG 
ATTCGTCCAG GATCTGTGGT CTCAAAGAAA GGTTACTTGA ACTTCAAAGA ACCTCTGTCT 
CATTCCTGGG TCAAGCATTT TGTGGTTGTG CGCCGTCCCT ATGTCTTCAT CTACAACAGT 
GACAAGGACC CAGTAGAGAG GGGCCTCATT AACTTATCCA CTGCACAAGT GGAGTACAGT 
GAAGACCAGC AAGCCATGGT TAAGAGCTCA AACATATTTG CTGTATGCAC AATGTACCGT 
GGGATCCTTC TGCAGGCCAT TAATGATAAA GATATGAATG ACTGGTTGTA TGCCTTTAAT 
CCTCTGCTTG CTGGCACAAT AAGGTCAAAG CTGGCAAGAC GTCGTTCTCT TTCCTTGAAG 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_031905976.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761836.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X4, mRNA.

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CloneID	OXa62407
Clone ID Related Accession (Same CDS sequence)	XM_031905976.1
Accession Version	XM_031905976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC ACCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCA 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGTT CTCCCATATT CCACGGCTGT 
GTGAATGAGC GGCTGGCAGA CCGCACCCCG TCCCCTACCT TCTCCACTGC AGACTCTGAT 
ATCACAGAGT TAGCTGATGA GCAGCAGGAC CAGATGGAAG ACTTTGATGA TGAGCCATTT 
GTGGATGACA CTGGCTCTGA TGTTGGTGCA GAAGAAGGAT CCGATCTCTT CAGTGATGGC 
CATGACCCGT TTTACGACCG GTCCCCATGG TTCATTTTAG TAGGAAGGGC TTTCGTCTAT 
CTAAGCAACC TGCTTTTCCC AGTTCCTCTT GTGCATCGAG CAGCTATAGT AAGTGAGAAA 
GGAGAAGTAC GTGGGTTTTT GCGTGTTGCT GTGCAGGCAA TATCTGGTGA TGAGGAAGCA 
CCAGACTATG GGTCCGGCAT TAGACAATCG GGAACAGCCA AGATTTCATT TGATAATGAA 
TACATTAATA AGAATGAAAT CTCTTCTATG GTTTTGACTC GTTCCGGACT GTCACTAGAA 
GAACTTCGCA TTGTGGAAGG ACAAGGACAG AACTCTGACA TTACTTCATT AGAAGAGGTC 
AATCATTTAG CTGATTTAGA CCTAAAGTCT GGAAACCTGT TGGATGGAAA GATGATGATG 
GAAGGATTCT CAGAGGATGT AGGAGAGCAT CTGAAAATTG GTAGCATGTT CACTTTCCGT 
GTGACAGTGC TTCAGGCCAG TGGAATTCCT CCAGAATATG CTGATATCTT CTGCCAATTT 
AACTTTTTAC ATCGACATGA TGAAGCATTT TCCACAGAGC CACTGAAGAA CAGTGGCAGA 
GGAAATCCTC TTGGTTTCTA TCATGTTCAG AATATTGCAG TGGAAATTAC AGAATCTTTT 
ATAGAATACA TTAAAAACAA CCCAATTGTT TTTGAAGTAT TTGGCCATTA CCAGCAGCAC 
CCTCTCCACC TTCAAGGACA AGATCTCAGC CCACCTCAGC AGTGCCGACG TTACTTTCCT 
CCTCCAATGC CACTTTCTAA ACCAGTTCCA GCCACTAAAT TAAATCCACT AAGCAAGCAA 
AGTCTTGGAC AGGTCATCAG CAAGTATGAC TTGTTAGTCT GGTTTGAGAT CAGTGAACTG 
GAGCCTACTG GAGAGTACAT TCCAGCTGTC GTAGATCATT CTGGAGGACT GCCCTGCCAG 
GGAACCTTTT TGCTGCATCA GGGAATCCAG CGCAGAATTA CTGTCACCAT TGTCCATGAG 
AAAGGGAATG AGTTGCACTG GAAGGATGTG AGAGAATTGG TTGTAGGACG TGTTCGCAAT 
AAAGCAGATG AAGCTGCTGC AGATGCTGTT CTTTCACTAA ATATCATCTC TGCAAAATAC 
CTCAGGTCTT CTCATAGCTC TGGCAGAACT TTTTATCGCT TTGAGGCAGT ATGGGACAGT 
TCCTTGCATA ACTCCCTGCT TCTAAACCGG GTAACTCCAT ATGGAGAGAA GATCTATATG 
ACCCTATCAG CGTATCTTGA GCTTGATCAC TGCATCCAGC CTGCAGTTAT TACAAAAGAT 
GTCTGTATGA TCTTTTACTC TCGTGATGCC AAAATATCTC CTCCACGGTC TCTGCGCAGT 
CTATTTGGCA GTGGTTACTC AAAATCCCCT GACTGTAACA GAGTAACTGG AATATATGAG 
TTGAGTTTGT GCAAAATGGC TGATACCGGC AGCCCTGGAA TGCAACGAAG AAGAAGAAAG 
ATCCTCGATA CGTCAGTGGC ATATGTTCGT GGTGAAGAGA ATCTGGCAGG ATGGAGACCT 
CGAGGCGATA GTCTAATCCT AGAGCATCAA TGGGAACTTG AAAAGATGGA GCAATTGCAT 
GAGGTTGAGA AAACAAGGCA CCTCCTTTTA CTTAGAGACA AACTAGGAGA CAGTATCCCT 
AAGTCCCTAA GTGAATCTAT CTCTCCAAGC TTGAGCAGTG GCACCCTAAG CACATCGACC 
AGTATTTCAT CGCAGATATC CACCACAACC TTTGAAAGTG CCATCACACC TAGTGAGAGC 
AGTGGATATG ACTCTACTGA TGTTGAGAGT CTTGTGGATC GTGAGAAGGA GTTAGCAACC 
AAGTGTTTGC GACTTTTGAC TCACACATTT AACCAAGAAC TAACCCAGGT GTGTAACAGT 
ATCAGTGACT GTAAGTTGTC TGATATCTCC CCAATTGGAA GGGATCCCTC GGTCTCCAGC 
TTTAGCAGTG CTACTCTCAC TCCTTCATCA ACTTGCCCAT CACTAGCAGA TTCTCGATGC 
AGCTCTGTGG ATCAGAAGAC CCCTGAAGCA AATTCACGTG CCTCCAGCCC TTGCACTGAA 
TTTGAACAGT TCCATTTGGT ACCAGCTGTG GAGCGGTCAT TTTTGGCTCG TGCAGGAAAG 
AACGAGTTTC TAAATCTTGT ACCAGATATT GAAGAGATTC GTCCAGGATC TGTGGTCTCA 
AAGAAAGGTT ACTTGAACTT CAAAGAACCT CTGTCTCATT CCTGGGTCAA GCATTTTGTG 
GTTGTGCGCC GTCCCTATGT CTTCATCTAC AACAGTGACA AGGACCCAGT AGAGAGGGGC 
CTCATTAACT TATCCACTGC ACAAGTGGAG TACAGTGAAG ACCAGCAAGC CATGGTTAAG 
AGCTCAAACA TATTTGCTGT ATGCACAATG TACCGTGGGA TCCTTCTGCA GGCCATTAAT 
GATAAAGATA TGAATGACTG GTTGTATGCC TTTAATCCTC TGCTTGCTGG CACAATAAGG 
TCAAAGCTGG CAAGACGTCG TTCTCTTTCC TTGAAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761836.1
CDS	330..5651
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_031905976.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905976.1

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC ACCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCA 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGTT CTCCCATATT CCACGGCTGT 
GTGAATGAGC GGCTGGCAGA CCGCACCCCG TCCCCTACCT TCTCCACTGC AGACTCTGAT 
ATCACAGAGT TAGCTGATGA GCAGCAGGAC CAGATGGAAG ACTTTGATGA TGAGCCATTT 
GTGGATGACA CTGGCTCTGA TGTTGGTGCA GAAGAAGGAT CCGATCTCTT CAGTGATGGC 
CATGACCCGT TTTACGACCG GTCCCCATGG TTCATTTTAG TAGGAAGGGC TTTCGTCTAT 
CTAAGCAACC TGCTTTTCCC AGTTCCTCTT GTGCATCGAG CAGCTATAGT AAGTGAGAAA 
GGAGAAGTAC GTGGGTTTTT GCGTGTTGCT GTGCAGGCAA TATCTGGTGA TGAGGAAGCA 
CCAGACTATG GGTCCGGCAT TAGACAATCG GGAACAGCCA AGATTTCATT TGATAATGAA 
TACATTAATA AGAATGAAAT CTCTTCTATG GTTTTGACTC GTTCCGGACT GTCACTAGAA 
GAACTTCGCA TTGTGGAAGG ACAAGGACAG AACTCTGACA TTACTTCATT AGAAGAGGTC 
AATCATTTAG CTGATTTAGA CCTAAAGTCT GGAAACCTGT TGGATGGAAA GATGATGATG 
GAAGGATTCT CAGAGGATGT AGGAGAGCAT CTGAAAATTG GTAGCATGTT CACTTTCCGT 
GTGACAGTGC TTCAGGCCAG TGGAATTCCT CCAGAATATG CTGATATCTT CTGCCAATTT 
AACTTTTTAC ATCGACATGA TGAAGCATTT TCCACAGAGC CACTGAAGAA CAGTGGCAGA 
GGAAATCCTC TTGGTTTCTA TCATGTTCAG AATATTGCAG TGGAAATTAC AGAATCTTTT 
ATAGAATACA TTAAAAACAA CCCAATTGTT TTTGAAGTAT TTGGCCATTA CCAGCAGCAC 
CCTCTCCACC TTCAAGGACA AGATCTCAGC CCACCTCAGC AGTGCCGACG TTACTTTCCT 
CCTCCAATGC CACTTTCTAA ACCAGTTCCA GCCACTAAAT TAAATCCACT AAGCAAGCAA 
AGTCTTGGAC AGGTCATCAG CAAGTATGAC TTGTTAGTCT GGTTTGAGAT CAGTGAACTG 
GAGCCTACTG GAGAGTACAT TCCAGCTGTC GTAGATCATT CTGGAGGACT GCCCTGCCAG 
GGAACCTTTT TGCTGCATCA GGGAATCCAG CGCAGAATTA CTGTCACCAT TGTCCATGAG 
AAAGGGAATG AGTTGCACTG GAAGGATGTG AGAGAATTGG TTGTAGGACG TGTTCGCAAT 
AAAGCAGATG AAGCTGCTGC AGATGCTGTT CTTTCACTAA ATATCATCTC TGCAAAATAC 
CTCAGGTCTT CTCATAGCTC TGGCAGAACT TTTTATCGCT TTGAGGCAGT ATGGGACAGT 
TCCTTGCATA ACTCCCTGCT TCTAAACCGG GTAACTCCAT ATGGAGAGAA GATCTATATG 
ACCCTATCAG CGTATCTTGA GCTTGATCAC TGCATCCAGC CTGCAGTTAT TACAAAAGAT 
GTCTGTATGA TCTTTTACTC TCGTGATGCC AAAATATCTC CTCCACGGTC TCTGCGCAGT 
CTATTTGGCA GTGGTTACTC AAAATCCCCT GACTGTAACA GAGTAACTGG AATATATGAG 
TTGAGTTTGT GCAAAATGGC TGATACCGGC AGCCCTGGAA TGCAACGAAG AAGAAGAAAG 
ATCCTCGATA CGTCAGTGGC ATATGTTCGT GGTGAAGAGA ATCTGGCAGG ATGGAGACCT 
CGAGGCGATA GTCTAATCCT AGAGCATCAA TGGGAACTTG AAAAGATGGA GCAATTGCAT 
GAGGTTGAGA AAACAAGGCA CCTCCTTTTA CTTAGAGACA AACTAGGAGA CAGTATCCCT 
AAGTCCCTAA GTGAATCTAT CTCTCCAAGC TTGAGCAGTG GCACCCTAAG CACATCGACC 
AGTATTTCAT CGCAGATATC CACCACAACC TTTGAAAGTG CCATCACACC TAGTGAGAGC 
AGTGGATATG ACTCTACTGA TGTTGAGAGT CTTGTGGATC GTGAGAAGGA GTTAGCAACC 
AAGTGTTTGC GACTTTTGAC TCACACATTT AACCAAGAAC TAACCCAGGT GTGTAACAGT 
ATCAGTGACT GTAAGTTGTC TGATATCTCC CCAATTGGAA GGGATCCCTC GGTCTCCAGC 
TTTAGCAGTG CTACTCTCAC TCCTTCATCA ACTTGCCCAT CACTAGCAGA TTCTCGATGC 
AGCTCTGTGG ATCAGAAGAC CCCTGAAGCA AATTCACGTG CCTCCAGCCC TTGCACTGAA 
TTTGAACAGT TCCATTTGGT ACCAGCTGTG GAGCGGTCAT TTTTGGCTCG TGCAGGAAAG 
AACGAGTTTC TAAATCTTGT ACCAGATATT GAAGAGATTC GTCCAGGATC TGTGGTCTCA 
AAGAAAGGTT ACTTGAACTT CAAAGAACCT CTGTCTCATT CCTGGGTCAA GCATTTTGTG 
GTTGTGCGCC GTCCCTATGT CTTCATCTAC AACAGTGACA AGGACCCAGT AGAGAGGGGC 
CTCATTAACT TATCCACTGC ACAAGTGGAG TACAGTGAAG ACCAGCAAGC CATGGTTAAG 
AGCTCAAACA TATTTGCTGT ATGCACAATG TACCGTGGGA TCCTTCTGCA GGCCATTAAT 
GATAAAGATA TGAATGACTG GTTGTATGCC TTTAATCCTC TGCTTGCTGG CACAATAAGG 
TCAAAGCTGG CAAGACGTCG TTCTCTTTCC TTGAAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966956.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822410.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X5, mRNA.

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CloneID	OXa62408
Clone ID Related Accession (Same CDS sequence)	XM_012966956.3
Accession Version	XM_012966956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CAGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCCACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822410.1
CDS	330..5633
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966956.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966956.3

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CAGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCCACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_018095819.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951308.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X6, mRNA.

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CloneID	OXa62409
Clone ID Related Accession (Same CDS sequence)	XM_018095819.2
Accession Version	XM_018095819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CACCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC AGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCCACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951308.1
CDS	331..5514
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_018095819.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095819.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CACCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC AGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCCACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966961.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822415.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X7, mRNA.

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CloneID	OXa62410
Clone ID Related Accession (Same CDS sequence)	XM_012966961.3
Accession Version	XM_012966961.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC ACCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCA 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCCACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822415.1
CDS	330..5402
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966961.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966961.3

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC ACCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCA 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCCACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_031905977.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761837.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X8, mRNA.

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CloneID	OXa62411
Clone ID Related Accession (Same CDS sequence)	XM_031905977.1
Accession Version	XM_031905977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CAGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG GGCTTTCGTC TATCTAAGCA ACCTGCTTTT CCCAGTTCCT 
CTTGTGCATC GAGCAGCTAT AGTAAGTGAG AAAGGAGAAG TACGTGGGTT TTTGCGTGTT 
GCTGTGCAGG CAATATCTGG TGATGAGGAA GCACCAGACT ATGGGTCCGG CATTAGACAA 
TCGGGAACAG CCAAGATTTC ATTTGATAAT GAATACATTA ATAAGAATGA AATCTCTTCT 
ATGGTTTTGA CTCGTTCCGG ACTGTCACTA GAAGAACTTC GCATTGTGGA AGGACAAGGA 
CAGAACTCTG ACATTACTTC ATTAGAAGAG GTCAATCATT TAGCTGATTT AGACCTAAAG 
TCTGGAAACC TGTTGGATGG AAAGATGATG ATGGAAGGAT TCTCAGAGGA TGTAGGAGAG 
CATCTGAAAA TTGGTAGCAT GTTCACTTTC CGTGTGACAG TGCTTCAGGC CAGTGGAATT 
CCTCCAGAAT ATGCTGATAT CTTCTGCCAA TTTAACTTTT TACATCGACA TGATGAAGCA 
TTTTCCACAG AGCCACTGAA GAACAGTGGC AGAGGAAATC CTCTTGGTTT CTATCATGTT 
CAGAATATTG CAGTGGAAAT TACAGAATCT TTTATAGAAT ACATTAAAAA CAACCCAATT 
GTTTTTGAAG TATTTGGCCA TTACCAGCAG CACCCTCTCC ACCTTCAAGG ACAAGATCTC 
AGCCCACCTC AGCAGTGCCG ACGTTACTTT CCTCCTCCAA TGCCACTTTC TAAACCAGTT 
CCAGCCACTA AATTAAATCC ACTAAGCAAG CAAAGTCTTG GACAGGTCAT CAGCAAGTAT 
GACTTGTTAG TCTGGTTTGA GATCAGTGAA CTGGAGCCTA CTGGAGAGTA CATTCCAGCT 
GTCGTAGATC ATTCTGGAGG ACTGCCCTGC CAGGGAACCT TTTTGCTGCA TCAGGGAATC 
CAGCGCAGAA TTACTGTCAC CATTGTCCAT GAGAAAGGGA ATGAGTTGCA CTGGAAGGAT 
GTGAGAGAAT TGGTTGTAGG ACGTGTTCGC AATAAAGCAG ATGAAGCTGC TGCAGATGCT 
GTTCTTTCAC TAAATATCAT CTCTGCAAAA TACCTCAGGT CTTCTCATAG CTCTGGCAGA 
ACTTTTTATC GCTTTGAGGC AGTATGGGAC AGTTCCTTGC ATAACTCCCT GCTTCTAAAC 
CGGGTAACTC CATATGGAGA GAAGATCTAT ATGACCCTAT CAGCGTATCT TGAGCTTGAT 
CACTGCATCC AGCCTGCAGT TATTACAAAA GATGTCTGTA TGATCTTTTA CTCTCGTGAT 
GCCAAAATAT CTCCTCCACG GTCTCTGCGC AGTCTATTTG GCAGTGGTTA CTCAAAATCC 
CCTGACTGTA ACAGAGTAAC TGGAATATAT GAGTTGAGTT TGTGCAAAAT GGCTGATACC 
GGCAGCCCTG GAATGCAACG AAGAAGAAGA AAGATCCTCG ATACGTCAGT GGCATATGTT 
CGTGGTGAAG AGAATCTGGC AGGATGGAGA CCTCGAGGCG ATAGTCTAAT CCTAGAGCAT 
CAATGGGAAC TTGAAAAGAT GGAGCAATTG CATGAGGTTG AGAAAACAAG GCACCTCCTT 
TTACTTAGAG ACAAACTAGG AGACAGTATC CCTAAGTCCC TAAGTGAATC TATCTCTCCA 
AGCTTGAGCA GTGGCACCCT AAGCACATCG ACCAGTATTT CATCGCAGAT ATCCACCACA 
ACCTTTGAAA GTGCCATCAC ACCTAGTGAG AGCAGTGGAT ATGACTCTAC TGATGTTGAG 
AGTCTTGTGG ATCGTGAGAA GGAGTTAGCA ACCAAGTGTT TGCGACTTTT GACTCACACA 
TTTAACCAAG AACTAACCCA GGTGTGTAAC AGTATCAGTG ACTGTAAGTT GTCTGATATC 
TCCCCAATTG GAAGGGATCC CTCGGTCTCC AGCTTTAGCA GTGCTACTCT CACTCCTTCA 
TCAACTTGCC CATCACTAGC AGATTCTCGA TGCAGCTCTG TGGATCAGAA GACCCCTGAA 
GCAAATTCAC GTGCCTCCAG CCCTTGCACT GAATTTGAAC AGTTCCATTT GGTACCAGCT 
GTGGAGCGGT CATTTTTGGC TCGTGCAGGA AAGAACGAGT TTCTAAATCT TGTACCAGAT 
ATTGAAGAGA TTCGTCCAGG ATCTGTGGTC TCAAAGAAAG GTTACTTGAA CTTCAAAGAA 
CCTCTGTCTC ATTCCTGGGT CAAGCATTTT GTGGTTGTGC GCCGTCCCTA TGTCTTCATC 
TACAACAGTG ACAAGGACCC AGTAGAGAGG GGCCTCATTA ACTTATCCAC TGCACAAGTG 
GAGTACAGTG AAGACCAGCA AGCCATGGTT AAGAGCTCAA ACATATTTGC TGTATGCACA 
ATGTACCGTG GGATCCTTCT GCAGGCCATT AATGATAAAG ATATGAATGA CTGGTTGTAT 
GCCTTTAATC CTCTGCTTGC TGGCACAATA AGGTCAAAGC TGGCAAGACG TCGTTCTCTT 
TCCTTGAAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761837.1
CDS	329..5383
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_031905977.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905977.1

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACACCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CAGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG GGCTTTCGTC TATCTAAGCA ACCTGCTTTT CCCAGTTCCT 
CTTGTGCATC GAGCAGCTAT AGTAAGTGAG AAAGGAGAAG TACGTGGGTT TTTGCGTGTT 
GCTGTGCAGG CAATATCTGG TGATGAGGAA GCACCAGACT ATGGGTCCGG CATTAGACAA 
TCGGGAACAG CCAAGATTTC ATTTGATAAT GAATACATTA ATAAGAATGA AATCTCTTCT 
ATGGTTTTGA CTCGTTCCGG ACTGTCACTA GAAGAACTTC GCATTGTGGA AGGACAAGGA 
CAGAACTCTG ACATTACTTC ATTAGAAGAG GTCAATCATT TAGCTGATTT AGACCTAAAG 
TCTGGAAACC TGTTGGATGG AAAGATGATG ATGGAAGGAT TCTCAGAGGA TGTAGGAGAG 
CATCTGAAAA TTGGTAGCAT GTTCACTTTC CGTGTGACAG TGCTTCAGGC CAGTGGAATT 
CCTCCAGAAT ATGCTGATAT CTTCTGCCAA TTTAACTTTT TACATCGACA TGATGAAGCA 
TTTTCCACAG AGCCACTGAA GAACAGTGGC AGAGGAAATC CTCTTGGTTT CTATCATGTT 
CAGAATATTG CAGTGGAAAT TACAGAATCT TTTATAGAAT ACATTAAAAA CAACCCAATT 
GTTTTTGAAG TATTTGGCCA TTACCAGCAG CACCCTCTCC ACCTTCAAGG ACAAGATCTC 
AGCCCACCTC AGCAGTGCCG ACGTTACTTT CCTCCTCCAA TGCCACTTTC TAAACCAGTT 
CCAGCCACTA AATTAAATCC ACTAAGCAAG CAAAGTCTTG GACAGGTCAT CAGCAAGTAT 
GACTTGTTAG TCTGGTTTGA GATCAGTGAA CTGGAGCCTA CTGGAGAGTA CATTCCAGCT 
GTCGTAGATC ATTCTGGAGG ACTGCCCTGC CAGGGAACCT TTTTGCTGCA TCAGGGAATC 
CAGCGCAGAA TTACTGTCAC CATTGTCCAT GAGAAAGGGA ATGAGTTGCA CTGGAAGGAT 
GTGAGAGAAT TGGTTGTAGG ACGTGTTCGC AATAAAGCAG ATGAAGCTGC TGCAGATGCT 
GTTCTTTCAC TAAATATCAT CTCTGCAAAA TACCTCAGGT CTTCTCATAG CTCTGGCAGA 
ACTTTTTATC GCTTTGAGGC AGTATGGGAC AGTTCCTTGC ATAACTCCCT GCTTCTAAAC 
CGGGTAACTC CATATGGAGA GAAGATCTAT ATGACCCTAT CAGCGTATCT TGAGCTTGAT 
CACTGCATCC AGCCTGCAGT TATTACAAAA GATGTCTGTA TGATCTTTTA CTCTCGTGAT 
GCCAAAATAT CTCCTCCACG GTCTCTGCGC AGTCTATTTG GCAGTGGTTA CTCAAAATCC 
CCTGACTGTA ACAGAGTAAC TGGAATATAT GAGTTGAGTT TGTGCAAAAT GGCTGATACC 
GGCAGCCCTG GAATGCAACG AAGAAGAAGA AAGATCCTCG ATACGTCAGT GGCATATGTT 
CGTGGTGAAG AGAATCTGGC AGGATGGAGA CCTCGAGGCG ATAGTCTAAT CCTAGAGCAT 
CAATGGGAAC TTGAAAAGAT GGAGCAATTG CATGAGGTTG AGAAAACAAG GCACCTCCTT 
TTACTTAGAG ACAAACTAGG AGACAGTATC CCTAAGTCCC TAAGTGAATC TATCTCTCCA 
AGCTTGAGCA GTGGCACCCT AAGCACATCG ACCAGTATTT CATCGCAGAT ATCCACCACA 
ACCTTTGAAA GTGCCATCAC ACCTAGTGAG AGCAGTGGAT ATGACTCTAC TGATGTTGAG 
AGTCTTGTGG ATCGTGAGAA GGAGTTAGCA ACCAAGTGTT TGCGACTTTT GACTCACACA 
TTTAACCAAG AACTAACCCA GGTGTGTAAC AGTATCAGTG ACTGTAAGTT GTCTGATATC 
TCCCCAATTG GAAGGGATCC CTCGGTCTCC AGCTTTAGCA GTGCTACTCT CACTCCTTCA 
TCAACTTGCC CATCACTAGC AGATTCTCGA TGCAGCTCTG TGGATCAGAA GACCCCTGAA 
GCAAATTCAC GTGCCTCCAG CCCTTGCACT GAATTTGAAC AGTTCCATTT GGTACCAGCT 
GTGGAGCGGT CATTTTTGGC TCGTGCAGGA AAGAACGAGT TTCTAAATCT TGTACCAGAT 
ATTGAAGAGA TTCGTCCAGG ATCTGTGGTC TCAAAGAAAG GTTACTTGAA CTTCAAAGAA 
CCTCTGTCTC ATTCCTGGGT CAAGCATTTT GTGGTTGTGC GCCGTCCCTA TGTCTTCATC 
TACAACAGTG ACAAGGACCC AGTAGAGAGG GGCCTCATTA ACTTATCCAC TGCACAAGTG 
GAGTACAGTG AAGACCAGCA AGCCATGGTT AAGAGCTCAA ACATATTTGC TGTATGCACA 
ATGTACCGTG GGATCCTTCT GCAGGCCATT AATGATAAAG ATATGAATGA CTGGTTGTAT 
GCCTTTAATC CTCTGCTTGC TGGCACAATA AGGTCAAAGC TGGCAAGACG TCGTTCTCTT 
TCCTTGAAGT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966950.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822404.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X1, mRNA.

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CloneID	OXa32932
Clone ID Related Accession (Same CDS sequence)	XM_012966950.2
Accession Version	XM_012966950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966950.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGGCT GTTTCTTGAT AAGGATATGC CTAGAACTTT TTATCGCTTT 
GAGGCAGTAT GGGACAGTTC CTTGCATAAC TCCCTGCTTC TAAACCGGGT AACTCCATAT 
GGAGAGAAGA TCTATATGAC CCTATCAGCG TATCTTGAGC TTGATCACTG CATCCAGCCT 
GCAGTTATTA CAAAAGATGT CTGTATGATC TTTTACTCTC GTGATGCCAA AATATCTCCT 
CCACGGTCTC TGCGCAGTCT ATTTGGCAGT GGTTACTCAA AATCCCCTGA CTGTAACAGA 
GTAACTGGAA TATATGAGTT GAGTTTGTGC AAAATGGCTG ATACCGGCAG CCCTGGAATG 
CAACGAAGAA GAAGAAAGAT CCTCGATACG TCAGTGGCAT ATGTTCGTGG TGAAGAGAAT 
CTGGCAGGAT GGAGACCTCG AGGCGATAGT CTAATCCTAG AGCATCAATG GGAACTTGAA 
AAGATGGAGC AATTGCATGA GGTTGAGAAA ACAAGGCACC TCCTTTTACT TAGAGACAAA 
CTAGGAGACA GTATCCCTAA GTCCCTAAGT GAATCTATCT CTCCAAGCTT GAGCAGTGGC 
ACCCTAAGCA CATCGACCAG TATTTCATCG CAGATATCCA CCACAACCTT TGAAAGTGCC 
ATCACACCTA GTGAGAGCAG TGGATATGAC TCTACTGATG TTGAGAGTCT TGTGGATCGT 
GAGAAGGAGT TAGCAACCAA GTGTTTGCGA CTTTTGACTC ACACATTTAA CCAAGAACTA 
ACCCAGGTGT GTAACAGTAT CAGTGACTGT AAGTTGTCTG ATATCTCCCC AATTGGAAGG 
GATCCCTCGG TCTCCAGCTT TAGCAGTGCT ACTCTCACTC CTTCATCAAC TTGCCCATCA 
CTAGCAGATT CTCGATGCAG CTCTGTGGAT CAGAAGACCC CTGAAGCAAA TTCACGTGCC 
TCCAGCCCTT GCACTGAATT TGAACAGTTC CATTTGGTAC CAGCTGTGGA GCGGTCATTT 
TTGGCTCGTG CAGGAAAGAA CGAGTTTCTA AATCTTGTAC CAGATATTGA AGAGATTCGT 
CCAGGATCTG TGGTCTCAAA GAAAGGTTAC TTGAACTTCA AAGAACCTCT GTCTCATTCC 
TGGGTCAAGC ATTTTGTGGT TGTGCGCCGT CCCTATGTCT TCATCTACAA CAGTGACAAG 
GACCCAGTAG AGAGGGGCCT CATTAACTTA TCCACTGCAC AAGTGGAGTA CAGTGAAGAC 
CAGCAAGCCA TGGTTAAGAG CTCAAACATA TTTGCTGTAT GCACAATGTA CCGTGGGATC 
CTTCTGCAGG CCATTAATGA TAAAGATATG AATGACTGGT TGTATGCCTT TAATCCTCTG 
CTTGCTGGCA CAATAAGGTC AAAGCTGGCA AGACGTCGTT CTCTTTCCTT GAAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822404.1
CDS	360..5819
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966950.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966950.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGGCT GTTTCTTGAT AAGGATATGC CTAGAACTTT TTATCGCTTT 
GAGGCAGTAT GGGACAGTTC CTTGCATAAC TCCCTGCTTC TAAACCGGGT AACTCCATAT 
GGAGAGAAGA TCTATATGAC CCTATCAGCG TATCTTGAGC TTGATCACTG CATCCAGCCT 
GCAGTTATTA CAAAAGATGT CTGTATGATC TTTTACTCTC GTGATGCCAA AATATCTCCT 
CCACGGTCTC TGCGCAGTCT ATTTGGCAGT GGTTACTCAA AATCCCCTGA CTGTAACAGA 
GTAACTGGAA TATATGAGTT GAGTTTGTGC AAAATGGCTG ATACCGGCAG CCCTGGAATG 
CAACGAAGAA GAAGAAAGAT CCTCGATACG TCAGTGGCAT ATGTTCGTGG TGAAGAGAAT 
CTGGCAGGAT GGAGACCTCG AGGCGATAGT CTAATCCTAG AGCATCAATG GGAACTTGAA 
AAGATGGAGC AATTGCATGA GGTTGAGAAA ACAAGGCACC TCCTTTTACT TAGAGACAAA 
CTAGGAGACA GTATCCCTAA GTCCCTAAGT GAATCTATCT CTCCAAGCTT GAGCAGTGGC 
ACCCTAAGCA CATCGACCAG TATTTCATCG CAGATATCCA CCACAACCTT TGAAAGTGCC 
ATCACACCTA GTGAGAGCAG TGGATATGAC TCTACTGATG TTGAGAGTCT TGTGGATCGT 
GAGAAGGAGT TAGCAACCAA GTGTTTGCGA CTTTTGACTC ACACATTTAA CCAAGAACTA 
ACCCAGGTGT GTAACAGTAT CAGTGACTGT AAGTTGTCTG ATATCTCCCC AATTGGAAGG 
GATCCCTCGG TCTCCAGCTT TAGCAGTGCT ACTCTCACTC CTTCATCAAC TTGCCCATCA 
CTAGCAGATT CTCGATGCAG CTCTGTGGAT CAGAAGACCC CTGAAGCAAA TTCACGTGCC 
TCCAGCCCTT GCACTGAATT TGAACAGTTC CATTTGGTAC CAGCTGTGGA GCGGTCATTT 
TTGGCTCGTG CAGGAAAGAA CGAGTTTCTA AATCTTGTAC CAGATATTGA AGAGATTCGT 
CCAGGATCTG TGGTCTCAAA GAAAGGTTAC TTGAACTTCA AAGAACCTCT GTCTCATTCC 
TGGGTCAAGC ATTTTGTGGT TGTGCGCCGT CCCTATGTCT TCATCTACAA CAGTGACAAG 
GACCCAGTAG AGAGGGGCCT CATTAACTTA TCCACTGCAC AAGTGGAGTA CAGTGAAGAC 
CAGCAAGCCA TGGTTAAGAG CTCAAACATA TTTGCTGTAT GCACAATGTA CCGTGGGATC 
CTTCTGCAGG CCATTAATGA TAAAGATATG AATGACTGGT TGTATGCCTT TAATCCTCTG 
CTTGCTGGCA CAATAAGGTC AAAGCTGGCA AGACGTCGTT CTCTTTCCTT GAAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966951.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822405.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X2, mRNA.

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CloneID	OXa32933
Clone ID Related Accession (Same CDS sequence)	XM_012966951.2
Accession Version	XM_012966951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966951.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACTCTTCCT TCTGTCGGGT GCTTTCCTAT CTCAGACATG 
AATGATAATG AGAACCTTAA GAATAAATAC TTATTAGCCA ATGAAAACCA ACGACCTGGC 
AATTTCTCCA CAGCCTCCAT CAGTTCTCTA GCATCCTCAC CATCCTCCTG TTCACTTAGT 
AGTCAGGTGG GATTGACATC TGTTACCAGC ATCCAGGAAA GAATCATGTC TACCCCAGGA 
GGAGAGGAAG CTATAGAGCG ACTTAAAGAG TCTGAAAAAA TAATTGCAGA GCTAAATGAG 
ACATGGGAAG AGAAGCTGCG AAAAACAGAG GCTATTCGAA TGGACAGGGA GGCTTTACTT 
GCAGAAATGG GAGTGGCTAT TCGTGAAGAT GGTGGAACCT TGGGGGTTTT CTCACCCAAA 
AAGACTCCCC ATCTTGTCAA TCTTAATGAA GATCCACTAA TGTCTGAATG CCTTCTATAC 
TACATTAAAG ATGGACTTAC AAGGGTCGGT CAGGCAGATG CTGATAGTCG CCAGGATATT 
GTGCTAAGTG GAGCTCATAT TAAAGAGGAA CACTGCATAT TCCGTAGTGA AAGGAATTCA 
AATGGGGAAG TAATAGTAAC CTTGGAGCCC GGTGACAGGT CAGAAACATA TGTCAATGGA 
AAACGAGTTG TGCATCCAGT GGAGTTAAGA TCTGGAAATC GAATAATAAT GGGAAAAAAC 
CATGTATTTC GTTTTAACCA CCCTGAGCAA GCCCGTGCTG AACGGGAGAA AACGCCTTCT 
GCAGAAACAC CCTCAGAACC AGTGGACTGG ACATTTGCTC AGCGTGAGCT GTTGGAGAAA 
CAAGGCATCG ATATGAAGCA AGAAATGGAG AAAAGGCTTC AGGAGATGGA AATTTTGTAC 
AAGAAAGAAA AGGAAGAGGC AGATCTTCTT TTGGAGCAAC AAAGGCTGGA CTATGAGAGC 
AAACTGCAAG CTCTGCAAAA ACAAGTCGAG ACTCACTCAC TGGCAGCAGA GACCACAGAT 
GATGGGGAAG AAGAAGAGGA GGAAGTTCCA TGGACACAGC ATGAATATGA TGTAGCACTG 
TGGGCATTTA AAAAGTGGAG AAATCACCAG TTCACCTCGT TACGGGACTT GCTTTGGGGG 
AATGCCATGT ATCTGAAGGA AGCCAATGCA ATAAGTGTAG AGCTGAAGAA GAAGGTGCAG 
TTCCAATTTG TGCTGCTTAC AGATACTTTG TACTCCCCAC TGCCACCAGA GTTACTTCCT 
TCTGATGCTG ATAAAACCAC AGAAGACAGG ACTTTCCCTC GAACTGTGGT TGCAGTTGAG 
GTGCAAGATT TAAAAAATGG GGCGACTCAT TACTGGTCAC TGGAGAAACT GAGGTTAAGG 
TTAGAACAGA TGCGGGAGAT GTATGACCGT GCGGGAGAAA TGGCTTCCCA TGTGCAAGAT 
GAAAATGACA CAGTGATAAC TGGAAATGAC CCTTTTTATG ATCGCTTTCA TTGGTTCAAG 
CTAGTTGGGA GTTCTCCCAT ATTCCACGGC TGTGTGAATG AGCGGCTGGC AGACCGCACC 
CCGTCCCCTA CCTTCTCCAC TGCAGACTCT GATATCACAG AGTTAGCTGA TGAGCAGCAG 
GACCAGATGG AAGACTTTGA TGATGAGCCA TTTGTGGATG ACACTGGCTC TGATGTTGGT 
GCAGAAGAAG GATCCGATCT CTTCAGTGAT GGCCATGACC CGTTTTACGA CCGGTCCCCA 
TGGTTCATTT TAGTAGGAAG GGCTTTCGTC TATCTAAGCA ACCTGCTTTT CCCAGTTCCT 
CTTGTGCATC GAGCAGCTAT AGTAAGTGAG AAAGGAGAAG TACGTGGGTT TTTGCGTGTT 
GCTGTGCAGG CAATATCTGG TGATGAGGAA GCACCAGACT ATGGGTCCGG CATTAGACAA 
TCGGGAACAG CCAAGATTTC ATTTGATAAT GAATACATTA ATAAGAATGA AATCTCTTCT 
ATGGTTTTGA CTCGTTCCGG ACTGTCACTA GAAGAACTTC GCATTGTGGA AGGACAAGGA 
CAGAACTCTG ACATTACTTC ATTAGAAGAG GTCAATCATT TAGCTGATTT AGACCTAAAG 
TCTGGAAACC TGTTGGATGG AAAGATGATG ATGGAAGGAT TCTCAGAGGA TGTAGGAGAG 
CATCTGAAAA TTGGTAGCAT GTTCACTTTC CGTGTGACAG TGCTTCAGGC CAGTGGAATT 
CCTCCAGAAT ATGCTGATAT CTTCTGCCAA TTTAACTTTT TACATCGACA TGATGAAGCA 
TTTTCTACAG AGCCACTGAA GAACAGTGGC AGAGGAAATC CTCTTGGTTT CTATCATGTT 
CAGAATATTG CAGTGGAAAT TACAGAATCT TTTATAGAAT ACATTAAAAA CAACCCAATT 
GTTTTTGAAG TATTTGGCCA TTACCAGCAG CACCCTCTCC ACCTTCAAGG ACAAGATCTC 
AGCCCACCTC AGCAGTGCCG ACGTTACTTT CCTCCTCCAA TGCCACTTTC TAAACCAGTT 
CCAGCCACTA AATTAAATCC ACTAAGCAAG CAAAGTCTTG GACAGGTCAT CAGCAAGTAT 
GACTTGTTAG TCTGGTTTGA GATCAGTGAA CTGGAGCCTA CTGGAGAGTA CATTCCAGCT 
GTCGTAGATC ATTCTGGAGG ACTGCCCTGC CAGGGAACCT TTTTGCTGCA TCAGGGAATC 
CAGCGCAGAA TTACTGTCAC CATTGTCCAT GAGAAAGGGA ATGAGTTGCA CTGGAAGGAT 
GTGAGAGAAT TGGTTGTAGG ACGTGTTCGC AATAAAGCAG ATGAAGCTGC TGCAGATGCT 
GTTCTTTCAC TAAATATCAT CTCTGCAAAA TACCTCAGGT CTTCTCATAG CTCTGGCAGG 
CTGTTTCTTG ATAAGGATAT GCCTAGAACT TTTTATCGCT TTGAGGCAGT ATGGGACAGT 
TCCTTGCATA ACTCCCTGCT TCTAAACCGG GTAACTCCAT ATGGAGAGAA GATCTATATG 
ACCCTATCAG CGTATCTTGA GCTTGATCAC TGCATCCAGC CTGCAGTTAT TACAAAAGAT 
GTCTGTATGA TCTTTTACTC TCGTGATGCC AAAATATCTC CTCCACGGTC TCTGCGCAGT 
CTATTTGGCA GTGGTTACTC AAAATCCCCT GACTGTAACA GAGTAACTGG AATATATGAG 
TTGAGTTTGT GCAAAATGGC TGATACCGGC AGCCCTGGAA TGCAACGAAG AAGAAGAAAG 
ATCCTCGATA CGTCAGTGGC ATATGTTCGT GGTGAAGAGA ATCTGGCAGG ATGGAGACCT 
CGAGGCGATA GTCTAATCCT AGAGCATCAA TGGGAACTTG AAAAGATGGA GCAATTGCAT 
GAGGTTGAGA AAACAAGGCA CCTCCTTTTA CTTAGAGACA AACTAGGAGA CAGTATCCCT 
AAGTCCCTAA GTGAATCTAT CTCTCCAAGC TTGAGCAGTG GCACCCTAAG CACATCGACC 
AGTATTTCAT CGCAGATATC CACCACAACC TTTGAAAGTG CCATCACACC TAGTGAGAGC 
AGTGGATATG ACTCTACTGA TGTTGAGAGT CTTGTGGATC GTGAGAAGGA GTTAGCAACC 
AAGTGTTTGC GACTTTTGAC TCACACATTT AACCAAGAAC TAACCCAGGT GTGTAACAGT 
ATCAGTGACT GTAAGTTGTC TGATATCTCC CCAATTGGAA GGGATCCCTC GGTCTCCAGC 
TTTAGCAGTG CTACTCTCAC TCCTTCATCA ACTTGCCCAT CACTAGCAGA TTCTCGATGC 
AGCTCTGTGG ATCAGAAGAC CCCTGAAGCA AATTCACGTG CCTCCAGCCC TTGCACTGAA 
TTTGAACAGT TCCATTTGGT ACCAGCTGTG GAGCGGTCAT TTTTGGCTCG TGCAGGAAAG 
AACGAGTTTC TAAATCTTGT ACCAGATATT GAAGAGATTC GTCCAGGATC TGTGGTCTCA 
AAGAAAGGTT ACTTGAACTT CAAAGAACCT CTGTCTCATT CCTGGGTCAA GCATTTTGTG 
GTTGTGCGCC GTCCCTATGT CTTCATCTAC AACAGTGACA AGGACCCAGT AGAGAGGGGC 
CTCATTAACT TATCCACTGC ACAAGTGGAG TACAGTGAAG ACCAGCAAGC CATGGTTAAG 
AGCTCAAACA TATTTGCTGT ATGCACAATG TACCGTGGGA TCCTTCTGCA GGCCATTAAT 
GATAAAGATA TGAATGACTG GTTGTATGCC TTTAATCCTC TGCTTGCTGG CACAATAAGG 
TCAAAGCTGG CAAGACGTCG TTCTCTTTCC TTGAAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822405.1
CDS	359..5800
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966951.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966951.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACTCTTCCT TCTGTCGGGT GCTTTCCTAT CTCAGACATG 
AATGATAATG AGAACCTTAA GAATAAATAC TTATTAGCCA ATGAAAACCA ACGACCTGGC 
AATTTCTCCA CAGCCTCCAT CAGTTCTCTA GCATCCTCAC CATCCTCCTG TTCACTTAGT 
AGTCAGGTGG GATTGACATC TGTTACCAGC ATCCAGGAAA GAATCATGTC TACCCCAGGA 
GGAGAGGAAG CTATAGAGCG ACTTAAAGAG TCTGAAAAAA TAATTGCAGA GCTAAATGAG 
ACATGGGAAG AGAAGCTGCG AAAAACAGAG GCTATTCGAA TGGACAGGGA GGCTTTACTT 
GCAGAAATGG GAGTGGCTAT TCGTGAAGAT GGTGGAACCT TGGGGGTTTT CTCACCCAAA 
AAGACTCCCC ATCTTGTCAA TCTTAATGAA GATCCACTAA TGTCTGAATG CCTTCTATAC 
TACATTAAAG ATGGACTTAC AAGGGTCGGT CAGGCAGATG CTGATAGTCG CCAGGATATT 
GTGCTAAGTG GAGCTCATAT TAAAGAGGAA CACTGCATAT TCCGTAGTGA AAGGAATTCA 
AATGGGGAAG TAATAGTAAC CTTGGAGCCC GGTGACAGGT CAGAAACATA TGTCAATGGA 
AAACGAGTTG TGCATCCAGT GGAGTTAAGA TCTGGAAATC GAATAATAAT GGGAAAAAAC 
CATGTATTTC GTTTTAACCA CCCTGAGCAA GCCCGTGCTG AACGGGAGAA AACGCCTTCT 
GCAGAAACAC CCTCAGAACC AGTGGACTGG ACATTTGCTC AGCGTGAGCT GTTGGAGAAA 
CAAGGCATCG ATATGAAGCA AGAAATGGAG AAAAGGCTTC AGGAGATGGA AATTTTGTAC 
AAGAAAGAAA AGGAAGAGGC AGATCTTCTT TTGGAGCAAC AAAGGCTGGA CTATGAGAGC 
AAACTGCAAG CTCTGCAAAA ACAAGTCGAG ACTCACTCAC TGGCAGCAGA GACCACAGAT 
GATGGGGAAG AAGAAGAGGA GGAAGTTCCA TGGACACAGC ATGAATATGA TGTAGCACTG 
TGGGCATTTA AAAAGTGGAG AAATCACCAG TTCACCTCGT TACGGGACTT GCTTTGGGGG 
AATGCCATGT ATCTGAAGGA AGCCAATGCA ATAAGTGTAG AGCTGAAGAA GAAGGTGCAG 
TTCCAATTTG TGCTGCTTAC AGATACTTTG TACTCCCCAC TGCCACCAGA GTTACTTCCT 
TCTGATGCTG ATAAAACCAC AGAAGACAGG ACTTTCCCTC GAACTGTGGT TGCAGTTGAG 
GTGCAAGATT TAAAAAATGG GGCGACTCAT TACTGGTCAC TGGAGAAACT GAGGTTAAGG 
TTAGAACAGA TGCGGGAGAT GTATGACCGT GCGGGAGAAA TGGCTTCCCA TGTGCAAGAT 
GAAAATGACA CAGTGATAAC TGGAAATGAC CCTTTTTATG ATCGCTTTCA TTGGTTCAAG 
CTAGTTGGGA GTTCTCCCAT ATTCCACGGC TGTGTGAATG AGCGGCTGGC AGACCGCACC 
CCGTCCCCTA CCTTCTCCAC TGCAGACTCT GATATCACAG AGTTAGCTGA TGAGCAGCAG 
GACCAGATGG AAGACTTTGA TGATGAGCCA TTTGTGGATG ACACTGGCTC TGATGTTGGT 
GCAGAAGAAG GATCCGATCT CTTCAGTGAT GGCCATGACC CGTTTTACGA CCGGTCCCCA 
TGGTTCATTT TAGTAGGAAG GGCTTTCGTC TATCTAAGCA ACCTGCTTTT CCCAGTTCCT 
CTTGTGCATC GAGCAGCTAT AGTAAGTGAG AAAGGAGAAG TACGTGGGTT TTTGCGTGTT 
GCTGTGCAGG CAATATCTGG TGATGAGGAA GCACCAGACT ATGGGTCCGG CATTAGACAA 
TCGGGAACAG CCAAGATTTC ATTTGATAAT GAATACATTA ATAAGAATGA AATCTCTTCT 
ATGGTTTTGA CTCGTTCCGG ACTGTCACTA GAAGAACTTC GCATTGTGGA AGGACAAGGA 
CAGAACTCTG ACATTACTTC ATTAGAAGAG GTCAATCATT TAGCTGATTT AGACCTAAAG 
TCTGGAAACC TGTTGGATGG AAAGATGATG ATGGAAGGAT TCTCAGAGGA TGTAGGAGAG 
CATCTGAAAA TTGGTAGCAT GTTCACTTTC CGTGTGACAG TGCTTCAGGC CAGTGGAATT 
CCTCCAGAAT ATGCTGATAT CTTCTGCCAA TTTAACTTTT TACATCGACA TGATGAAGCA 
TTTTCTACAG AGCCACTGAA GAACAGTGGC AGAGGAAATC CTCTTGGTTT CTATCATGTT 
CAGAATATTG CAGTGGAAAT TACAGAATCT TTTATAGAAT ACATTAAAAA CAACCCAATT 
GTTTTTGAAG TATTTGGCCA TTACCAGCAG CACCCTCTCC ACCTTCAAGG ACAAGATCTC 
AGCCCACCTC AGCAGTGCCG ACGTTACTTT CCTCCTCCAA TGCCACTTTC TAAACCAGTT 
CCAGCCACTA AATTAAATCC ACTAAGCAAG CAAAGTCTTG GACAGGTCAT CAGCAAGTAT 
GACTTGTTAG TCTGGTTTGA GATCAGTGAA CTGGAGCCTA CTGGAGAGTA CATTCCAGCT 
GTCGTAGATC ATTCTGGAGG ACTGCCCTGC CAGGGAACCT TTTTGCTGCA TCAGGGAATC 
CAGCGCAGAA TTACTGTCAC CATTGTCCAT GAGAAAGGGA ATGAGTTGCA CTGGAAGGAT 
GTGAGAGAAT TGGTTGTAGG ACGTGTTCGC AATAAAGCAG ATGAAGCTGC TGCAGATGCT 
GTTCTTTCAC TAAATATCAT CTCTGCAAAA TACCTCAGGT CTTCTCATAG CTCTGGCAGG 
CTGTTTCTTG ATAAGGATAT GCCTAGAACT TTTTATCGCT TTGAGGCAGT ATGGGACAGT 
TCCTTGCATA ACTCCCTGCT TCTAAACCGG GTAACTCCAT ATGGAGAGAA GATCTATATG 
ACCCTATCAG CGTATCTTGA GCTTGATCAC TGCATCCAGC CTGCAGTTAT TACAAAAGAT 
GTCTGTATGA TCTTTTACTC TCGTGATGCC AAAATATCTC CTCCACGGTC TCTGCGCAGT 
CTATTTGGCA GTGGTTACTC AAAATCCCCT GACTGTAACA GAGTAACTGG AATATATGAG 
TTGAGTTTGT GCAAAATGGC TGATACCGGC AGCCCTGGAA TGCAACGAAG AAGAAGAAAG 
ATCCTCGATA CGTCAGTGGC ATATGTTCGT GGTGAAGAGA ATCTGGCAGG ATGGAGACCT 
CGAGGCGATA GTCTAATCCT AGAGCATCAA TGGGAACTTG AAAAGATGGA GCAATTGCAT 
GAGGTTGAGA AAACAAGGCA CCTCCTTTTA CTTAGAGACA AACTAGGAGA CAGTATCCCT 
AAGTCCCTAA GTGAATCTAT CTCTCCAAGC TTGAGCAGTG GCACCCTAAG CACATCGACC 
AGTATTTCAT CGCAGATATC CACCACAACC TTTGAAAGTG CCATCACACC TAGTGAGAGC 
AGTGGATATG ACTCTACTGA TGTTGAGAGT CTTGTGGATC GTGAGAAGGA GTTAGCAACC 
AAGTGTTTGC GACTTTTGAC TCACACATTT AACCAAGAAC TAACCCAGGT GTGTAACAGT 
ATCAGTGACT GTAAGTTGTC TGATATCTCC CCAATTGGAA GGGATCCCTC GGTCTCCAGC 
TTTAGCAGTG CTACTCTCAC TCCTTCATCA ACTTGCCCAT CACTAGCAGA TTCTCGATGC 
AGCTCTGTGG ATCAGAAGAC CCCTGAAGCA AATTCACGTG CCTCCAGCCC TTGCACTGAA 
TTTGAACAGT TCCATTTGGT ACCAGCTGTG GAGCGGTCAT TTTTGGCTCG TGCAGGAAAG 
AACGAGTTTC TAAATCTTGT ACCAGATATT GAAGAGATTC GTCCAGGATC TGTGGTCTCA 
AAGAAAGGTT ACTTGAACTT CAAAGAACCT CTGTCTCATT CCTGGGTCAA GCATTTTGTG 
GTTGTGCGCC GTCCCTATGT CTTCATCTAC AACAGTGACA AGGACCCAGT AGAGAGGGGC 
CTCATTAACT TATCCACTGC ACAAGTGGAG TACAGTGAAG ACCAGCAAGC CATGGTTAAG 
AGCTCAAACA TATTTGCTGT ATGCACAATG TACCGTGGGA TCCTTCTGCA GGCCATTAAT 
GATAAAGATA TGAATGACTG GTTGTATGCC TTTAATCCTC TGCTTGCTGG CACAATAAGG 
TCAAAGCTGG CAAGACGTCG TTCTCTTTCC TTGAAGTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966952.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822406.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X3, mRNA.

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CloneID	OXa32934
Clone ID Related Accession (Same CDS sequence)	XM_012966952.2
Accession Version	XM_012966952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822406.1
CDS	359..5791
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966952.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966952.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966953.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822407.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X4, mRNA.

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CloneID	OXa32935
Clone ID Related Accession (Same CDS sequence)	XM_012966953.2
Accession Version	XM_012966953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGGCT GTTTCTTGAT AAGGATATGC CTAGAACTTT TTATCGCTTT 
GAGGCAGTAT GGGACAGTTC CTTGCATAAC TCCCTGCTTC TAAACCGGGT AACTCCATAT 
GGAGAGAAGA TCTATATGAC CCTATCAGCG TATCTTGAGC TTGATCACTG CATCCAGCCT 
GCAGTTATTA CAAAAGATGT CTGTATGATC TTTTACTCTC GTGATGCCAA AATATCTCCT 
CCACGGTCTC TGCGCAGTCT ATTTGGCAGT GGTTACTCAA AATCCCCTGA CTGTAACAGA 
GTAACTGGAA TATATGAGTT GAGTTTGTGC AAAATGGCTG ATACCGGCAG CCCTGGAATG 
CAACGAAGAA GAAGAAAGAT CCTCGATACG TCAGTGGCAT ATGTTCGTGG TGAAGAGAAT 
CTGGCAGGAT GGAGACCTCG AGGCGATAGT CTAATCCTAG AGCATCAATG GGAACTTGAA 
AAGATGGAGC AATTGCATGA GGTTGAGAAA ACAAGGCACC TCCTTTTACT TAGAGACAAA 
CTAGGAGACA GTATCCCTAA GTCCCTAAGT GAATCTATCT CTCCAAGCTT GAGCAGTGGC 
ACCCTAAGCA CATCGACCAG TATTTCATCG CAGATATCCA CCACAACCTT TGAAAGTGCC 
ATCACACCTA GTGAGAGCAG TGGATATGAC TCTACTGATG TTGAGAGTCT TGTGGATCGT 
GAGAAGGAGT TAGCAACCAA GTGTTTGCGA CTTTTGACTC ACACATTTAA CCAAGAACTA 
ACCCAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822407.1
CDS	360..5792
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966953.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966953.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGT TCTCCCATAT TCCACGGCTG TGTGAATGAG 
CGGCTGGCAG ACCGCACCCC GTCCCCTACC TTCTCCACTG CAGACTCTGA TATCACAGAG 
TTAGCTGATG AGCAGCAGGA CCAGATGGAA GACTTTGATG ATGAGCCATT TGTGGATGAC 
ACTGGCTCTG ATGTTGGTGC AGAAGAAGGA TCCGATCTCT TCAGTGATGG CCATGACCCG 
TTTTACGACC GGTCCCCATG GTTCATTTTA GTAGGAAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGGCT GTTTCTTGAT AAGGATATGC CTAGAACTTT TTATCGCTTT 
GAGGCAGTAT GGGACAGTTC CTTGCATAAC TCCCTGCTTC TAAACCGGGT AACTCCATAT 
GGAGAGAAGA TCTATATGAC CCTATCAGCG TATCTTGAGC TTGATCACTG CATCCAGCCT 
GCAGTTATTA CAAAAGATGT CTGTATGATC TTTTACTCTC GTGATGCCAA AATATCTCCT 
CCACGGTCTC TGCGCAGTCT ATTTGGCAGT GGTTACTCAA AATCCCCTGA CTGTAACAGA 
GTAACTGGAA TATATGAGTT GAGTTTGTGC AAAATGGCTG ATACCGGCAG CCCTGGAATG 
CAACGAAGAA GAAGAAAGAT CCTCGATACG TCAGTGGCAT ATGTTCGTGG TGAAGAGAAT 
CTGGCAGGAT GGAGACCTCG AGGCGATAGT CTAATCCTAG AGCATCAATG GGAACTTGAA 
AAGATGGAGC AATTGCATGA GGTTGAGAAA ACAAGGCACC TCCTTTTACT TAGAGACAAA 
CTAGGAGACA GTATCCCTAA GTCCCTAAGT GAATCTATCT CTCCAAGCTT GAGCAGTGGC 
ACCCTAAGCA CATCGACCAG TATTTCATCG CAGATATCCA CCACAACCTT TGAAAGTGCC 
ATCACACCTA GTGAGAGCAG TGGATATGAC TCTACTGATG TTGAGAGTCT TGTGGATCGT 
GAGAAGGAGT TAGCAACCAA GTGTTTGCGA CTTTTGACTC ACACATTTAA CCAAGAACTA 
ACCCAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966954.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822408.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X5, mRNA.

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CloneID	OXa32936
Clone ID Related Accession (Same CDS sequence)	XM_012966954.2
Accession Version	XM_012966954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966954.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGTT CTCCCATATT CCACGGCTGT 
GTGAATGAGC GGCTGGCAGA CCGCACCCCG TCCCCTACCT TCTCCACTGC AGACTCTGAT 
ATCACAGAGT TAGCTGATGA GCAGCAGGAC CAGATGGAAG ACTTTGATGA TGAGCCATTT 
GTGGATGACA CTGGCTCTGA TGTTGGTGCA GAAGAAGGAT CCGATCTCTT CAGTGATGGC 
CATGACCCGT TTTACGACCG GTCCCCATGG TTCATTTTAG TAGGAAGGGC TTTCGTCTAT 
CTAAGCAACC TGCTTTTCCC AGTTCCTCTT GTGCATCGAG CAGCTATAGT AAGTGAGAAA 
GGAGAAGTAC GTGGGTTTTT GCGTGTTGCT GTGCAGGCAA TATCTGGTGA TGAGGAAGCA 
CCAGACTATG GGTCCGGCAT TAGACAATCG GGAACAGCCA AGATTTCATT TGATAATGAA 
TACATTAATA AGAATGAAAT CTCTTCTATG GTTTTGACTC GTTCCGGACT GTCACTAGAA 
GAACTTCGCA TTGTGGAAGG ACAAGGACAG AACTCTGACA TTACTTCATT AGAAGAGGTC 
AATCATTTAG CTGATTTAGA CCTAAAGTCT GGAAACCTGT TGGATGGAAA GATGATGATG 
GAAGGATTCT CAGAGGATGT AGGAGAGCAT CTGAAAATTG GTAGCATGTT CACTTTCCGT 
GTGACAGTGC TTCAGGCCAG TGGAATTCCT CCAGAATATG CTGATATCTT CTGCCAATTT 
AACTTTTTAC ATCGACATGA TGAAGCATTT TCTACAGAGC CACTGAAGAA CAGTGGCAGA 
GGAAATCCTC TTGGTTTCTA TCATGTTCAG AATATTGCAG TGGAAATTAC AGAATCTTTT 
ATAGAATACA TTAAAAACAA CCCAATTGTT TTTGAAGTAT TTGGCCATTA CCAGCAGCAC 
CCTCTCCACC TTCAAGGACA AGATCTCAGC CCACCTCAGC AGTGCCGACG TTACTTTCCT 
CCTCCAATGC CACTTTCTAA ACCAGTTCCA GCCACTAAAT TAAATCCACT AAGCAAGCAA 
AGTCTTGGAC AGGTCATCAG CAAGTATGAC TTGTTAGTCT GGTTTGAGAT CAGTGAACTG 
GAGCCTACTG GAGAGTACAT TCCAGCTGTC GTAGATCATT CTGGAGGACT GCCCTGCCAG 
GGAACCTTTT TGCTGCATCA GGGAATCCAG CGCAGAATTA CTGTCACCAT TGTCCATGAG 
AAAGGGAATG AGTTGCACTG GAAGGATGTG AGAGAATTGG TTGTAGGACG TGTTCGCAAT 
AAAGCAGATG AAGCTGCTGC AGATGCTGTT CTTTCACTAA ATATCATCTC TGCAAAATAC 
CTCAGGTCTT CTCATAGCTC TGGCAGGCTG TTTCTTGATA AGGATATGCC TAGAACTTTT 
TATCGCTTTG AGGCAGTATG GGACAGTTCC TTGCATAACT CCCTGCTTCT AAACCGGGTA 
ACTCCATATG GAGAGAAGAT CTATATGACC CTATCAGCGT ATCTTGAGCT TGATCACTGC 
ATCCAGCCTG CAGTTATTAC AAAAGATGTC TGTATGATCT TTTACTCTCG TGATGCCAAA 
ATATCTCCTC CACGGTCTCT GCGCAGTCTA TTTGGCAGTG GTTACTCAAA ATCCCCTGAC 
TGTAACAGAG TAACTGGAAT ATATGAGTTG AGTTTGTGCA AAATGGCTGA TACCGGCAGC 
CCTGGAATGC AACGAAGAAG AAGAAAGATC CTCGATACGT CAGTGGCATA TGTTCGTGGT 
GAAGAGAATC TGGCAGGATG GAGACCTCGA GGCGATAGTC TAATCCTAGA GCATCAATGG 
GAACTTGAAA AGATGGAGCA ATTGCATGAG GTTGAGAAAA CAAGGCACCT CCTTTTACTT 
AGAGACAAAC TAGGAGACAG TATCCCTAAG TCCCTAAGTG AATCTATCTC TCCAAGCTTG 
AGCAGTGGCA CCCTAAGCAC ATCGACCAGT ATTTCATCGC AGATATCCAC CACAACCTTT 
GAAAGTGCCA TCACACCTAG TGAGAGCAGT GGATATGACT CTACTGATGT TGAGAGTCTT 
GTGGATCGTG AGAAGGAGTT AGCAACCAAG TGTTTGCGAC TTTTGACTCA CACATTTAAC 
CAAGAACTAA CCCAGGTGTG TAACAGTATC AGTGACTGTA AGTTGTCTGA TATCTCCCCA 
ATTGGAAGGG ATCCCTCGGT CTCCAGCTTT AGCAGTGCTA CTCTCACTCC TTCATCAACT 
TGCCCATCAC TAGCAGATTC TCGATGCAGC TCTGTGGATC AGAAGACCCC TGAAGCAAAT 
TCACGTGCCT CCAGCCCTTG CACTGAATTT GAACAGTTCC ATTTGGTACC AGCTGTGGAG 
CGGTCATTTT TGGCTCGTGC AGGAAAGAAC GAGTTTCTAA ATCTTGTACC AGATATTGAA 
GAGATTCGTC CAGGATCTGT GGTCTCAAAG AAAGGTTACT TGAACTTCAA AGAACCTCTG 
TCTCATTCCT GGGTCAAGCA TTTTGTGGTT GTGCGCCGTC CCTATGTCTT CATCTACAAC 
AGTGACAAGG ACCCAGTAGA GAGGGGCCTC ATTAACTTAT CCACTGCACA AGTGGAGTAC 
AGTGAAGACC AGCAAGCCAT GGTTAAGAGC TCAAACATAT TTGCTGTATG CACAATGTAC 
CGTGGGATCC TTCTGCAGGC CATTAATGAT AAAGATATGA ATGACTGGTT GTATGCCTTT 
AATCCTCTGC TTGCTGGCAC AATAAGGTCA AAGCTGGCAA GACGTCGTTC TCTTTCCTTG 
AAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822408.1
CDS	358..5706
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966954.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966954.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGTT CTCCCATATT CCACGGCTGT 
GTGAATGAGC GGCTGGCAGA CCGCACCCCG TCCCCTACCT TCTCCACTGC AGACTCTGAT 
ATCACAGAGT TAGCTGATGA GCAGCAGGAC CAGATGGAAG ACTTTGATGA TGAGCCATTT 
GTGGATGACA CTGGCTCTGA TGTTGGTGCA GAAGAAGGAT CCGATCTCTT CAGTGATGGC 
CATGACCCGT TTTACGACCG GTCCCCATGG TTCATTTTAG TAGGAAGGGC TTTCGTCTAT 
CTAAGCAACC TGCTTTTCCC AGTTCCTCTT GTGCATCGAG CAGCTATAGT AAGTGAGAAA 
GGAGAAGTAC GTGGGTTTTT GCGTGTTGCT GTGCAGGCAA TATCTGGTGA TGAGGAAGCA 
CCAGACTATG GGTCCGGCAT TAGACAATCG GGAACAGCCA AGATTTCATT TGATAATGAA 
TACATTAATA AGAATGAAAT CTCTTCTATG GTTTTGACTC GTTCCGGACT GTCACTAGAA 
GAACTTCGCA TTGTGGAAGG ACAAGGACAG AACTCTGACA TTACTTCATT AGAAGAGGTC 
AATCATTTAG CTGATTTAGA CCTAAAGTCT GGAAACCTGT TGGATGGAAA GATGATGATG 
GAAGGATTCT CAGAGGATGT AGGAGAGCAT CTGAAAATTG GTAGCATGTT CACTTTCCGT 
GTGACAGTGC TTCAGGCCAG TGGAATTCCT CCAGAATATG CTGATATCTT CTGCCAATTT 
AACTTTTTAC ATCGACATGA TGAAGCATTT TCTACAGAGC CACTGAAGAA CAGTGGCAGA 
GGAAATCCTC TTGGTTTCTA TCATGTTCAG AATATTGCAG TGGAAATTAC AGAATCTTTT 
ATAGAATACA TTAAAAACAA CCCAATTGTT TTTGAAGTAT TTGGCCATTA CCAGCAGCAC 
CCTCTCCACC TTCAAGGACA AGATCTCAGC CCACCTCAGC AGTGCCGACG TTACTTTCCT 
CCTCCAATGC CACTTTCTAA ACCAGTTCCA GCCACTAAAT TAAATCCACT AAGCAAGCAA 
AGTCTTGGAC AGGTCATCAG CAAGTATGAC TTGTTAGTCT GGTTTGAGAT CAGTGAACTG 
GAGCCTACTG GAGAGTACAT TCCAGCTGTC GTAGATCATT CTGGAGGACT GCCCTGCCAG 
GGAACCTTTT TGCTGCATCA GGGAATCCAG CGCAGAATTA CTGTCACCAT TGTCCATGAG 
AAAGGGAATG AGTTGCACTG GAAGGATGTG AGAGAATTGG TTGTAGGACG TGTTCGCAAT 
AAAGCAGATG AAGCTGCTGC AGATGCTGTT CTTTCACTAA ATATCATCTC TGCAAAATAC 
CTCAGGTCTT CTCATAGCTC TGGCAGGCTG TTTCTTGATA AGGATATGCC TAGAACTTTT 
TATCGCTTTG AGGCAGTATG GGACAGTTCC TTGCATAACT CCCTGCTTCT AAACCGGGTA 
ACTCCATATG GAGAGAAGAT CTATATGACC CTATCAGCGT ATCTTGAGCT TGATCACTGC 
ATCCAGCCTG CAGTTATTAC AAAAGATGTC TGTATGATCT TTTACTCTCG TGATGCCAAA 
ATATCTCCTC CACGGTCTCT GCGCAGTCTA TTTGGCAGTG GTTACTCAAA ATCCCCTGAC 
TGTAACAGAG TAACTGGAAT ATATGAGTTG AGTTTGTGCA AAATGGCTGA TACCGGCAGC 
CCTGGAATGC AACGAAGAAG AAGAAAGATC CTCGATACGT CAGTGGCATA TGTTCGTGGT 
GAAGAGAATC TGGCAGGATG GAGACCTCGA GGCGATAGTC TAATCCTAGA GCATCAATGG 
GAACTTGAAA AGATGGAGCA ATTGCATGAG GTTGAGAAAA CAAGGCACCT CCTTTTACTT 
AGAGACAAAC TAGGAGACAG TATCCCTAAG TCCCTAAGTG AATCTATCTC TCCAAGCTTG 
AGCAGTGGCA CCCTAAGCAC ATCGACCAGT ATTTCATCGC AGATATCCAC CACAACCTTT 
GAAAGTGCCA TCACACCTAG TGAGAGCAGT GGATATGACT CTACTGATGT TGAGAGTCTT 
GTGGATCGTG AGAAGGAGTT AGCAACCAAG TGTTTGCGAC TTTTGACTCA CACATTTAAC 
CAAGAACTAA CCCAGGTGTG TAACAGTATC AGTGACTGTA AGTTGTCTGA TATCTCCCCA 
ATTGGAAGGG ATCCCTCGGT CTCCAGCTTT AGCAGTGCTA CTCTCACTCC TTCATCAACT 
TGCCCATCAC TAGCAGATTC TCGATGCAGC TCTGTGGATC AGAAGACCCC TGAAGCAAAT 
TCACGTGCCT CCAGCCCTTG CACTGAATTT GAACAGTTCC ATTTGGTACC AGCTGTGGAG 
CGGTCATTTT TGGCTCGTGC AGGAAAGAAC GAGTTTCTAA ATCTTGTACC AGATATTGAA 
GAGATTCGTC CAGGATCTGT GGTCTCAAAG AAAGGTTACT TGAACTTCAA AGAACCTCTG 
TCTCATTCCT GGGTCAAGCA TTTTGTGGTT GTGCGCCGTC CCTATGTCTT CATCTACAAC 
AGTGACAAGG ACCCAGTAGA GAGGGGCCTC ATTAACTTAT CCACTGCACA AGTGGAGTAC 
AGTGAAGACC AGCAAGCCAT GGTTAAGAGC TCAAACATAT TTGCTGTATG CACAATGTAC 
CGTGGGATCC TTCTGCAGGC CATTAATGAT AAAGATATGA ATGACTGGTT GTATGCCTTT 
AATCCTCTGC TTGCTGGCAC AATAAGGTCA AAGCTGGCAA GACGTCGTTC TCTTTCCTTG 
AAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966955.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822409.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X6, mRNA.

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CloneID	OXa32937
Clone ID Related Accession (Same CDS sequence)	XM_012966955.2
Accession Version	XM_012966955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CGGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGGC TGTTTCTTGA TAAGGATATG CCTAGAACTT TTTATCGCTT TGAGGCAGTA 
TGGGACAGTT CCTTGCATAA CTCCCTGCTT CTAAACCGGG TAACTCCATA TGGAGAGAAG 
ATCTATATGA CCCTATCAGC GTATCTTGAG CTTGATCACT GCATCCAGCC TGCAGTTATT 
ACAAAAGATG TCTGTATGAT CTTTTACTCT CGTGATGCCA AAATATCTCC TCCACGGTCT 
CTGCGCAGTC TATTTGGCAG TGGTTACTCA AAATCCCCTG ACTGTAACAG AGTAACTGGA 
ATATATGAGT TGAGTTTGTG CAAAATGGCT GATACCGGCA GCCCTGGAAT GCAACGAAGA 
AGAAGAAAGA TCCTCGATAC GTCAGTGGCA TATGTTCGTG GTGAAGAGAA TCTGGCAGGA 
TGGAGACCTC GAGGCGATAG TCTAATCCTA GAGCATCAAT GGGAACTTGA AAAGATGGAG 
CAATTGCATG AGGTTGAGAA AACAAGGCAC CTCCTTTTAC TTAGAGACAA ACTAGGAGAC 
AGTATCCCTA AGTCCCTAAG TGAATCTATC TCTCCAAGCT TGAGCAGTGG CACCCTAAGC 
ACATCGACCA GTATTTCATC GCAGATATCC ACCACAACCT TTGAAAGTGC CATCACACCT 
AGTGAGAGCA GTGGATATGA CTCTACTGAT GTTGAGAGTC TTGTGGATCG TGAGAAGGAG 
TTAGCAACCA AGTGTTTGCG ACTTTTGACT CACACATTTA ACCAAGAACT AACCCAGGTG 
TGTAACAGTA TCAGTGACTG TAAGTTGTCT GATATCTCCC CAATTGGAAG GGATCCCTCG 
GTCTCCAGCT TTAGCAGTGC TACTCTCACT CCTTCATCAA CTTGCCCATC ACTAGCAGAT 
TCTCGATGCA GCTCTGTGGA TCAGAAGACC CCTGAAGCAA ATTCACGTGC CTCCAGCCCT 
TGCACTGAAT TTGAACAGTT CCATTTGGTA CCAGCTGTGG AGCGGTCATT TTTGGCTCGT 
GCAGGAAAGA ACGAGTTTCT AAATCTTGTA CCAGATATTG AAGAGATTCG TCCAGGATCT 
GTGGTCTCAA AGAAAGGTTA CTTGAACTTC AAAGAACCTC TGTCTCATTC CTGGGTCAAG 
CATTTTGTGG TTGTGCGCCG TCCCTATGTC TTCATCTACA ACAGTGACAA GGACCCAGTA 
GAGAGGGGCC TCATTAACTT ATCCACTGCA CAAGTGGAGT ACAGTGAAGA CCAGCAAGCC 
ATGGTTAAGA GCTCAAACAT ATTTGCTGTA TGCACAATGT ACCGTGGGAT CCTTCTGCAG 
GCCATTAATG ATAAAGATAT GAATGACTGG TTGTATGCCT TTAATCCTCT GCTTGCTGGC 
ACAATAAGGT CAAAGCTGGC AAGACGTCGT TCTCTTTCCT TGAAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822409.1
CDS	357..5687
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966955.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966955.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CGGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGGC TGTTTCTTGA TAAGGATATG CCTAGAACTT TTTATCGCTT TGAGGCAGTA 
TGGGACAGTT CCTTGCATAA CTCCCTGCTT CTAAACCGGG TAACTCCATA TGGAGAGAAG 
ATCTATATGA CCCTATCAGC GTATCTTGAG CTTGATCACT GCATCCAGCC TGCAGTTATT 
ACAAAAGATG TCTGTATGAT CTTTTACTCT CGTGATGCCA AAATATCTCC TCCACGGTCT 
CTGCGCAGTC TATTTGGCAG TGGTTACTCA AAATCCCCTG ACTGTAACAG AGTAACTGGA 
ATATATGAGT TGAGTTTGTG CAAAATGGCT GATACCGGCA GCCCTGGAAT GCAACGAAGA 
AGAAGAAAGA TCCTCGATAC GTCAGTGGCA TATGTTCGTG GTGAAGAGAA TCTGGCAGGA 
TGGAGACCTC GAGGCGATAG TCTAATCCTA GAGCATCAAT GGGAACTTGA AAAGATGGAG 
CAATTGCATG AGGTTGAGAA AACAAGGCAC CTCCTTTTAC TTAGAGACAA ACTAGGAGAC 
AGTATCCCTA AGTCCCTAAG TGAATCTATC TCTCCAAGCT TGAGCAGTGG CACCCTAAGC 
ACATCGACCA GTATTTCATC GCAGATATCC ACCACAACCT TTGAAAGTGC CATCACACCT 
AGTGAGAGCA GTGGATATGA CTCTACTGAT GTTGAGAGTC TTGTGGATCG TGAGAAGGAG 
TTAGCAACCA AGTGTTTGCG ACTTTTGACT CACACATTTA ACCAAGAACT AACCCAGGTG 
TGTAACAGTA TCAGTGACTG TAAGTTGTCT GATATCTCCC CAATTGGAAG GGATCCCTCG 
GTCTCCAGCT TTAGCAGTGC TACTCTCACT CCTTCATCAA CTTGCCCATC ACTAGCAGAT 
TCTCGATGCA GCTCTGTGGA TCAGAAGACC CCTGAAGCAA ATTCACGTGC CTCCAGCCCT 
TGCACTGAAT TTGAACAGTT CCATTTGGTA CCAGCTGTGG AGCGGTCATT TTTGGCTCGT 
GCAGGAAAGA ACGAGTTTCT AAATCTTGTA CCAGATATTG AAGAGATTCG TCCAGGATCT 
GTGGTCTCAA AGAAAGGTTA CTTGAACTTC AAAGAACCTC TGTCTCATTC CTGGGTCAAG 
CATTTTGTGG TTGTGCGCCG TCCCTATGTC TTCATCTACA ACAGTGACAA GGACCCAGTA 
GAGAGGGGCC TCATTAACTT ATCCACTGCA CAAGTGGAGT ACAGTGAAGA CCAGCAAGCC 
ATGGTTAAGA GCTCAAACAT ATTTGCTGTA TGCACAATGT ACCGTGGGAT CCTTCTGCAG 
GCCATTAATG ATAAAGATAT GAATGACTGG TTGTATGCCT TTAATCCTCT GCTTGCTGGC 
ACAATAAGGT CAAAGCTGGC AAGACGTCGT TCTCTTTCCT TGAAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966956.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822410.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X7, mRNA.

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CloneID	OXa32938
Clone ID Related Accession (Same CDS sequence)	XM_012966956.2
Accession Version	XM_012966956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CGGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822410.1
CDS	357..5660
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966956.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966956.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGAGTAAG AAGAAGAAAA AAACAGACTT CATTCCATAT 
CGAGATTCTG TACTTACATG GCTGCTTCGT GAGAACTTAG GTGGCAATTC ACGGACAGCA 
ATGGTTGCAG CTCTGAGTCC TGCCGATATA AACTATGATG AGACTCTAAG TACATTAAGA 
TATGCAGATC GTGCTAAACA GATTAAGTGC AATGCTGTTA TCAATGAAGA TCCCAATGCC 
AAACTTGTGC GTGAGCTAAA AGATGAAGTA ACAAGGCTTA AAGACCTCTT GCGTGCTCAG 
GGATTGGGCG ATATCCTTGA TACCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CGGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGAA CTTTTTATCG CTTTGAGGCA GTATGGGACA GTTCCTTGCA TAACTCCCTG 
CTTCTAAACC GGGTAACTCC ATATGGAGAG AAGATCTATA TGACCCTATC AGCGTATCTT 
GAGCTTGATC ACTGCATCCA GCCTGCAGTT ATTACAAAAG ATGTCTGTAT GATCTTTTAC 
TCTCGTGATG CCAAAATATC TCCTCCACGG TCTCTGCGCA GTCTATTTGG CAGTGGTTAC 
TCAAAATCCC CTGACTGTAA CAGAGTAACT GGAATATATG AGTTGAGTTT GTGCAAAATG 
GCTGATACCG GCAGCCCTGG AATGCAACGA AGAAGAAGAA AGATCCTCGA TACGTCAGTG 
GCATATGTTC GTGGTGAAGA GAATCTGGCA GGATGGAGAC CTCGAGGCGA TAGTCTAATC 
CTAGAGCATC AATGGGAACT TGAAAAGATG GAGCAATTGC ATGAGGTTGA GAAAACAAGG 
CACCTCCTTT TACTTAGAGA CAAACTAGGA GACAGTATCC CTAAGTCCCT AAGTGAATCT 
ATCTCTCCAA GCTTGAGCAG TGGCACCCTA AGCACATCGA CCAGTATTTC ATCGCAGATA 
TCCACCACAA CCTTTGAAAG TGCCATCACA CCTAGTGAGA GCAGTGGATA TGACTCTACT 
GATGTTGAGA GTCTTGTGGA TCGTGAGAAG GAGTTAGCAA CCAAGTGTTT GCGACTTTTG 
ACTCACACAT TTAACCAAGA ACTAACCCAG GTGTGTAACA GTATCAGTGA CTGTAAGTTG 
TCTGATATCT CCCCAATTGG AAGGGATCCC TCGGTCTCCA GCTTTAGCAG TGCTACTCTC 
ACTCCTTCAT CAACTTGCCC ATCACTAGCA GATTCTCGAT GCAGCTCTGT GGATCAGAAG 
ACCCCTGAAG CAAATTCACG TGCCTCCAGC CCTTGCACTG AATTTGAACA GTTCCATTTG 
GTACCAGCTG TGGAGCGGTC ATTTTTGGCT CGTGCAGGAA AGAACGAGTT TCTAAATCTT 
GTACCAGATA TTGAAGAGAT TCGTCCAGGA TCTGTGGTCT CAAAGAAAGG TTACTTGAAC 
TTCAAAGAAC CTCTGTCTCA TTCCTGGGTC AAGCATTTTG TGGTTGTGCG CCGTCCCTAT 
GTCTTCATCT ACAACAGTGA CAAGGACCCA GTAGAGAGGG GCCTCATTAA CTTATCCACT 
GCACAAGTGG AGTACAGTGA AGACCAGCAA GCCATGGTTA AGAGCTCAAA CATATTTGCT 
GTATGCACAA TGTACCGTGG GATCCTTCTG CAGGCCATTA ATGATAAAGA TATGAATGAC 
TGGTTGTATG CCTTTAATCC TCTGCTTGCT GGCACAATAA GGTCAAAGCT GGCAAGACGT 
CGTTCTCTTT CCTTGAAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966957.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822411.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X8, mRNA.

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CloneID	OXa32939
Clone ID Related Accession (Same CDS sequence)	XM_012966957.2
Accession Version	XM_012966957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966957.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGTT CTCCCATATT CCACGGCTGT 
GTGAATGAGC GGCTGGCAGA CCGCACCCCG TCCCCTACCT TCTCCACTGC AGACTCTGAT 
ATCACAGAGT TAGCTGATGA GCAGCAGGAC CAGATGGAAG ACTTTGATGA TGAGCCATTT 
GTGGATGACA CTGGCTCTGA TGTTGGTGCA GAAGAAGGAT CCGATCTCTT CAGTGATGGC 
CATGACCCGT TTTACGACCG GTCCCCATGG TTCATTTTAG TAGGAAGGGC TTTCGTCTAT 
CTAAGCAACC TGCTTTTCCC AGTTCCTCTT GTGCATCGAG CAGCTATAGT AAGTGAGAAA 
GGAGAAGTAC GTGGGTTTTT GCGTGTTGCT GTGCAGGCAA TATCTGGTGA TGAGGAAGCA 
CCAGACTATG GGTCCGGCAT TAGACAATCG GGAACAGCCA AGATTTCATT TGATAATGAA 
TACATTAATA AGAATGAAAT CTCTTCTATG GTTTTGACTC GTTCCGGACT GTCACTAGAA 
GAACTTCGCA TTGTGGAAGG ACAAGGACAG AACTCTGACA TTACTTCATT AGAAGAGGTC 
AATCATTTAG CTGATTTAGA CCTAAAGTCT GGAAACCTGT TGGATGGAAA GATGATGATG 
GAAGGATTCT CAGAGGATGT AGGAGAGCAT CTGAAAATTG GTAGCATGTT CACTTTCCGT 
GTGACAGTGC TTCAGGCCAG TGGAATTCCT CCAGAATATG CTGATATCTT CTGCCAATTT 
AACTTTTTAC ATCGACATGA TGAAGCATTT TCTACAGAGC CACTGAAGAA CAGTGGCAGA 
GGAAATCCTC TTGGTTTCTA TCATGTTCAG AATATTGCAG TGGAAATTAC AGAATCTTTT 
ATAGAATACA TTAAAAACAA CCCAATTGTT TTTGAAGTAT TTGGCCATTA CCAGCAGCAC 
CCTCTCCACC TTCAAGGACA AGATCTCAGC CCACCTCAGC AGTGCCGACG TTACTTTCCT 
CCTCCAATGC CACTTTCTAA ACCAGTTCCA GCCACTAAAT TAAATCCACT AAGCAAGCAA 
AGTCTTGGAC AGGTCATCAG CAAGTATGAC TTGTTAGTCT GGTTTGAGAT CAGTGAACTG 
GAGCCTACTG GAGAGTACAT TCCAGCTGTC GTAGATCATT CTGGAGGACT GCCCTGCCAG 
GGAACCTTTT TGCTGCATCA GGGAATCCAG CGCAGAATTA CTGTCACCAT TGTCCATGAG 
AAAGGGAATG AGTTGCACTG GAAGGATGTG AGAGAATTGG TTGTAGGACG TGTTCGCAAT 
AAAGCAGATG AAGCTGCTGC AGATGCTGTT CTTTCACTAA ATATCATCTC TGCAAAATAC 
CTCAGGTCTT CTCATAGCTC TGGCAGAACT TTTTATCGCT TTGAGGCAGT ATGGGACAGT 
TCCTTGCATA ACTCCCTGCT TCTAAACCGG GTAACTCCAT ATGGAGAGAA GATCTATATG 
ACCCTATCAG CGTATCTTGA GCTTGATCAC TGCATCCAGC CTGCAGTTAT TACAAAAGAT 
GTCTGTATGA TCTTTTACTC TCGTGATGCC AAAATATCTC CTCCACGGTC TCTGCGCAGT 
CTATTTGGCA GTGGTTACTC AAAATCCCCT GACTGTAACA GAGTAACTGG AATATATGAG 
TTGAGTTTGT GCAAAATGGC TGATACCGGC AGCCCTGGAA TGCAACGAAG AAGAAGAAAG 
ATCCTCGATA CGTCAGTGGC ATATGTTCGT GGTGAAGAGA ATCTGGCAGG ATGGAGACCT 
CGAGGCGATA GTCTAATCCT AGAGCATCAA TGGGAACTTG AAAAGATGGA GCAATTGCAT 
GAGGTTGAGA AAACAAGGCA CCTCCTTTTA CTTAGAGACA AACTAGGAGA CAGTATCCCT 
AAGTCCCTAA GTGAATCTAT CTCTCCAAGC TTGAGCAGTG GCACCCTAAG CACATCGACC 
AGTATTTCAT CGCAGATATC CACCACAACC TTTGAAAGTG CCATCACACC TAGTGAGAGC 
AGTGGATATG ACTCTACTGA TGTTGAGAGT CTTGTGGATC GTGAGAAGGA GTTAGCAACC 
AAGTGTTTGC GACTTTTGAC TCACACATTT AACCAAGAAC TAACCCAGGT GTGTAACAGT 
ATCAGTGACT GTAAGTTGTC TGATATCTCC CCAATTGGAA GGGATCCCTC GGTCTCCAGC 
TTTAGCAGTG CTACTCTCAC TCCTTCATCA ACTTGCCCAT CACTAGCAGA TTCTCGATGC 
AGCTCTGTGG ATCAGAAGAC CCCTGAAGCA AATTCACGTG CCTCCAGCCC TTGCACTGAA 
TTTGAACAGT TCCATTTGGT ACCAGCTGTG GAGCGGTCAT TTTTGGCTCG TGCAGGAAAG 
AACGAGTTTC TAAATCTTGT ACCAGATATT GAAGAGATTC GTCCAGGATC TGTGGTCTCA 
AAGAAAGGTT ACTTGAACTT CAAAGAACCT CTGTCTCATT CCTGGGTCAA GCATTTTGTG 
GTTGTGCGCC GTCCCTATGT CTTCATCTAC AACAGTGACA AGGACCCAGT AGAGAGGGGC 
CTCATTAACT TATCCACTGC ACAAGTGGAG TACAGTGAAG ACCAGCAAGC CATGGTTAAG 
AGCTCAAACA TATTTGCTGT ATGCACAATG TACCGTGGGA TCCTTCTGCA GGCCATTAAT 
GATAAAGATA TGAATGACTG GTTGTATGCC TTTAATCCTC TGCTTGCTGG CACAATAAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822411.1
CDS	358..5640
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIR

Target ORF information:

RefSeq Version	XM_012966957.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966957.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGTT CTCCCATATT CCACGGCTGT 
GTGAATGAGC GGCTGGCAGA CCGCACCCCG TCCCCTACCT TCTCCACTGC AGACTCTGAT 
ATCACAGAGT TAGCTGATGA GCAGCAGGAC CAGATGGAAG ACTTTGATGA TGAGCCATTT 
GTGGATGACA CTGGCTCTGA TGTTGGTGCA GAAGAAGGAT CCGATCTCTT CAGTGATGGC 
CATGACCCGT TTTACGACCG GTCCCCATGG TTCATTTTAG TAGGAAGGGC TTTCGTCTAT 
CTAAGCAACC TGCTTTTCCC AGTTCCTCTT GTGCATCGAG CAGCTATAGT AAGTGAGAAA 
GGAGAAGTAC GTGGGTTTTT GCGTGTTGCT GTGCAGGCAA TATCTGGTGA TGAGGAAGCA 
CCAGACTATG GGTCCGGCAT TAGACAATCG GGAACAGCCA AGATTTCATT TGATAATGAA 
TACATTAATA AGAATGAAAT CTCTTCTATG GTTTTGACTC GTTCCGGACT GTCACTAGAA 
GAACTTCGCA TTGTGGAAGG ACAAGGACAG AACTCTGACA TTACTTCATT AGAAGAGGTC 
AATCATTTAG CTGATTTAGA CCTAAAGTCT GGAAACCTGT TGGATGGAAA GATGATGATG 
GAAGGATTCT CAGAGGATGT AGGAGAGCAT CTGAAAATTG GTAGCATGTT CACTTTCCGT 
GTGACAGTGC TTCAGGCCAG TGGAATTCCT CCAGAATATG CTGATATCTT CTGCCAATTT 
AACTTTTTAC ATCGACATGA TGAAGCATTT TCTACAGAGC CACTGAAGAA CAGTGGCAGA 
GGAAATCCTC TTGGTTTCTA TCATGTTCAG AATATTGCAG TGGAAATTAC AGAATCTTTT 
ATAGAATACA TTAAAAACAA CCCAATTGTT TTTGAAGTAT TTGGCCATTA CCAGCAGCAC 
CCTCTCCACC TTCAAGGACA AGATCTCAGC CCACCTCAGC AGTGCCGACG TTACTTTCCT 
CCTCCAATGC CACTTTCTAA ACCAGTTCCA GCCACTAAAT TAAATCCACT AAGCAAGCAA 
AGTCTTGGAC AGGTCATCAG CAAGTATGAC TTGTTAGTCT GGTTTGAGAT CAGTGAACTG 
GAGCCTACTG GAGAGTACAT TCCAGCTGTC GTAGATCATT CTGGAGGACT GCCCTGCCAG 
GGAACCTTTT TGCTGCATCA GGGAATCCAG CGCAGAATTA CTGTCACCAT TGTCCATGAG 
AAAGGGAATG AGTTGCACTG GAAGGATGTG AGAGAATTGG TTGTAGGACG TGTTCGCAAT 
AAAGCAGATG AAGCTGCTGC AGATGCTGTT CTTTCACTAA ATATCATCTC TGCAAAATAC 
CTCAGGTCTT CTCATAGCTC TGGCAGAACT TTTTATCGCT TTGAGGCAGT ATGGGACAGT 
TCCTTGCATA ACTCCCTGCT TCTAAACCGG GTAACTCCAT ATGGAGAGAA GATCTATATG 
ACCCTATCAG CGTATCTTGA GCTTGATCAC TGCATCCAGC CTGCAGTTAT TACAAAAGAT 
GTCTGTATGA TCTTTTACTC TCGTGATGCC AAAATATCTC CTCCACGGTC TCTGCGCAGT 
CTATTTGGCA GTGGTTACTC AAAATCCCCT GACTGTAACA GAGTAACTGG AATATATGAG 
TTGAGTTTGT GCAAAATGGC TGATACCGGC AGCCCTGGAA TGCAACGAAG AAGAAGAAAG 
ATCCTCGATA CGTCAGTGGC ATATGTTCGT GGTGAAGAGA ATCTGGCAGG ATGGAGACCT 
CGAGGCGATA GTCTAATCCT AGAGCATCAA TGGGAACTTG AAAAGATGGA GCAATTGCAT 
GAGGTTGAGA AAACAAGGCA CCTCCTTTTA CTTAGAGACA AACTAGGAGA CAGTATCCCT 
AAGTCCCTAA GTGAATCTAT CTCTCCAAGC TTGAGCAGTG GCACCCTAAG CACATCGACC 
AGTATTTCAT CGCAGATATC CACCACAACC TTTGAAAGTG CCATCACACC TAGTGAGAGC 
AGTGGATATG ACTCTACTGA TGTTGAGAGT CTTGTGGATC GTGAGAAGGA GTTAGCAACC 
AAGTGTTTGC GACTTTTGAC TCACACATTT AACCAAGAAC TAACCCAGGT GTGTAACAGT 
ATCAGTGACT GTAAGTTGTC TGATATCTCC CCAATTGGAA GGGATCCCTC GGTCTCCAGC 
TTTAGCAGTG CTACTCTCAC TCCTTCATCA ACTTGCCCAT CACTAGCAGA TTCTCGATGC 
AGCTCTGTGG ATCAGAAGAC CCCTGAAGCA AATTCACGTG CCTCCAGCCC TTGCACTGAA 
TTTGAACAGT TCCATTTGGT ACCAGCTGTG GAGCGGTCAT TTTTGGCTCG TGCAGGAAAG 
AACGAGTTTC TAAATCTTGT ACCAGATATT GAAGAGATTC GTCCAGGATC TGTGGTCTCA 
AAGAAAGGTT ACTTGAACTT CAAAGAACCT CTGTCTCATT CCTGGGTCAA GCATTTTGTG 
GTTGTGCGCC GTCCCTATGT CTTCATCTAC AACAGTGACA AGGACCCAGT AGAGAGGGGC 
CTCATTAACT TATCCACTGC ACAAGTGGAG TACAGTGAAG ACCAGCAAGC CATGGTTAAG 
AGCTCAAACA TATTTGCTGT ATGCACAATG TACCGTGGGA TCCTTCTGCA GGCCATTAAT 
GATAAAGATA TGAATGACTG GTTGTATGCC TTTAATCCTC TGCTTGCTGG CACAATAAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966958.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822412.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X9, mRNA.

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CloneID	OXa32940
Clone ID Related Accession (Same CDS sequence)	XM_012966958.2
Accession Version	XM_012966958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966958.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGGC TGTTTCTTGA TAAGGATATG CCTAGAACTT TTTATCGCTT TGAGGCAGTA 
TGGGACAGTT CCTTGCATAA CTCCCTGCTT CTAAACCGGG TAACTCCATA TGGAGAGAAG 
ATCTATATGA CCCTATCAGC GTATCTTGAG CTTGATCACT GCATCCAGCC TGCAGTTATT 
ACAAAAGATG TCTGTATGAT CTTTTACTCT CGTGATGCCA AAATATCTCC TCCACGGTCT 
CTGCGCAGTC TATTTGGCAG TGGTTACTCA AAATCCCCTG ACTGTAACAG AGTAACTGGA 
ATATATGAGT TGAGTTTGTG CAAAATGGCT GATACCGGCA GCCCTGGAAT GCAACGAAGA 
AGAAGAAAGA TCCTCGATAC GTCAGTGGCA TATGTTCGTG GTGAAGAGAA TCTGGCAGGA 
TGGAGACCTC GAGGCGATAG TCTAATCCTA GAGCATCAAT GGGAACTTGA AAAGATGGAG 
CAATTGCATG AGGTTGAGAA AACAAGGCAC CTCCTTTTAC TTAGAGACAA ACTAGGAGAC 
AGTATCCCTA AGTCCCTAAG TGAATCTATC TCTCCAAGCT TGAGCAGTGG CACCCTAAGC 
ACATCGACCA GTATTTCATC GCAGATATCC ACCACAACCT TTGAAAGTGC CATCACACCT 
AGTGAGAGCA GTGGATATGA CTCTACTGAT GTTGAGAGTC TTGTGGATCG TGAGAAGGAG 
TTAGCAACCA AGTGTTTGCG ACTTTTGACT CACACATTTA ACCAAGAACT AACCCAGGTG 
TGTAACAGTA TCAGTGACTG TAAGTTGTCT GATATCTCCC CAATTGGAAG GGATCCCTCG 
GTCTCCAGCT TTAGCAGTGC TACTCTCACT CCTTCATCAA CTTGCCCATC ACTAGCAGAT 
TCTCGATGCA GCTCTGTGGA TCAGAAGACC CCTGAAGCAA ATTCACGTGC CTCCAGCCCT 
TGCACTGAAT TTGAACAGTT CCATTTGGTA CCAGCTGTGG AGCGGTCATT TTTGGCTCGT 
GCAGGAAAGA ACGAGTTTCT AAATCTTGTA CCAGATATTG AAGAGATTCG TCCAGGATCT 
GTGGTCTCAA AGAAAGGTTA CTTGAACTTC AAAGAACCTC TGTCTCATTC CTGGGTCAAG 
CATTTTGTGG TTGTGCGCCG TCCCTATGTC TTCATCTACA ACAGTGACAA GGACCCAGTA 
GAGAGGGGCC TCATTAACTT ATCCACTGCA CAAGTGGAGT ACAGTGAAGA CCAGCAAGCC 
ATGGTTAAGA GCTCAAACAT ATTTGCTGTA TGCACAATGT ACCGTGGGAT CCTTCTGCAG 
GCCATTAATG ATAAAGATAT GAATGACTGG TTGTATGCCT TTAATCCTCT GCTTGCTGGC 
ACAATAAGGT CAAAGCTGGC AAGACGTCGT TCTCTTTCCT TGAAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822412.1
CDS	359..5569
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966958.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966958.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CTCTTCCTTC TGTCGGGTGC 
TTTCCTATCT CAGACATGAA TGATAATGAG AACCTTAAGA ATAAATACTT ATTAGCCAAT 
GAAAACCAAC GACCTGGCAA TTTCTCCACA GCCTCCATCA GTTCTCTAGC ATCCTCACCA 
TCCTCCTGTT CACTTAGTAG TCAGGTGGGA TTGACATCTG TTACCAGCAT CCAGGAAAGA 
ATCATGTCTA CCCCAGGAGG AGAGGAAGCT ATAGAGCGAC TTAAAGAGTC TGAAAAAATA 
ATTGCAGAGC TAAATGAGAC ATGGGAAGAG AAGCTGCGAA AAACAGAGGC TATTCGAATG 
GACAGGGAGG CTTTACTTGC AGAAATGGGA GTGGCTATTC GTGAAGATGG TGGAACCTTG 
GGGGTTTTCT CACCCAAAAA GACTCCCCAT CTTGTCAATC TTAATGAAGA TCCACTAATG 
TCTGAATGCC TTCTATACTA CATTAAAGAT GGACTTACAA GGGTCGGTCA GGCAGATGCT 
GATAGTCGCC AGGATATTGT GCTAAGTGGA GCTCATATTA AAGAGGAACA CTGCATATTC 
CGTAGTGAAA GGAATTCAAA TGGGGAAGTA ATAGTAACCT TGGAGCCCGG TGACAGGTCA 
GAAACATATG TCAATGGAAA ACGAGTTGTG CATCCAGTGG AGTTAAGATC TGGAAATCGA 
ATAATAATGG GAAAAAACCA TGTATTTCGT TTTAACCACC CTGAGCAAGC CCGTGCTGAA 
CGGGAGAAAA CGCCTTCTGC AGAAACACCC TCAGAACCAG TGGACTGGAC ATTTGCTCAG 
CGTGAGCTGT TGGAGAAACA AGGCATCGAT ATGAAGCAAG AAATGGAGAA AAGGCTTCAG 
GAGATGGAAA TTTTGTACAA GAAAGAAAAG GAAGAGGCAG ATCTTCTTTT GGAGCAACAA 
AGGCTGGACT ATGAGAGCAA ACTGCAAGCT CTGCAAAAAC AAGTCGAGAC TCACTCACTG 
GCAGCAGAGA CCACAGATGA TGGGGAAGAA GAAGAGGAGG AAGTTCCATG GACACAGCAT 
GAATATGATG TAGCACTGTG GGCATTTAAA AAGTGGAGAA ATCACCAGTT CACCTCGTTA 
CGGGACTTGC TTTGGGGGAA TGCCATGTAT CTGAAGGAAG CCAATGCAAT AAGTGTAGAG 
CTGAAGAAGA AGGTGCAGTT CCAATTTGTG CTGCTTACAG ATACTTTGTA CTCCCCACTG 
CCACCAGAGT TACTTCCTTC TGATGCTGAT AAAACCACAG AAGACAGGAC TTTCCCTCGA 
ACTGTGGTTG CAGTTGAGGT GCAAGATTTA AAAAATGGGG CGACTCATTA CTGGTCACTG 
GAGAAACTGA GGTTAAGGTT AGAACAGATG CGGGAGATGT ATGACCGTGC GGGAGAAATG 
GCTTCCCATG TGCAAGATGA AAATGACACA GTGATAACTG GAAATGACCC TTTTTATGAT 
CGCTTTCATT GGTTCAAGCT AGTTGGGAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGGC TGTTTCTTGA TAAGGATATG CCTAGAACTT TTTATCGCTT TGAGGCAGTA 
TGGGACAGTT CCTTGCATAA CTCCCTGCTT CTAAACCGGG TAACTCCATA TGGAGAGAAG 
ATCTATATGA CCCTATCAGC GTATCTTGAG CTTGATCACT GCATCCAGCC TGCAGTTATT 
ACAAAAGATG TCTGTATGAT CTTTTACTCT CGTGATGCCA AAATATCTCC TCCACGGTCT 
CTGCGCAGTC TATTTGGCAG TGGTTACTCA AAATCCCCTG ACTGTAACAG AGTAACTGGA 
ATATATGAGT TGAGTTTGTG CAAAATGGCT GATACCGGCA GCCCTGGAAT GCAACGAAGA 
AGAAGAAAGA TCCTCGATAC GTCAGTGGCA TATGTTCGTG GTGAAGAGAA TCTGGCAGGA 
TGGAGACCTC GAGGCGATAG TCTAATCCTA GAGCATCAAT GGGAACTTGA AAAGATGGAG 
CAATTGCATG AGGTTGAGAA AACAAGGCAC CTCCTTTTAC TTAGAGACAA ACTAGGAGAC 
AGTATCCCTA AGTCCCTAAG TGAATCTATC TCTCCAAGCT TGAGCAGTGG CACCCTAAGC 
ACATCGACCA GTATTTCATC GCAGATATCC ACCACAACCT TTGAAAGTGC CATCACACCT 
AGTGAGAGCA GTGGATATGA CTCTACTGAT GTTGAGAGTC TTGTGGATCG TGAGAAGGAG 
TTAGCAACCA AGTGTTTGCG ACTTTTGACT CACACATTTA ACCAAGAACT AACCCAGGTG 
TGTAACAGTA TCAGTGACTG TAAGTTGTCT GATATCTCCC CAATTGGAAG GGATCCCTCG 
GTCTCCAGCT TTAGCAGTGC TACTCTCACT CCTTCATCAA CTTGCCCATC ACTAGCAGAT 
TCTCGATGCA GCTCTGTGGA TCAGAAGACC CCTGAAGCAA ATTCACGTGC CTCCAGCCCT 
TGCACTGAAT TTGAACAGTT CCATTTGGTA CCAGCTGTGG AGCGGTCATT TTTGGCTCGT 
GCAGGAAAGA ACGAGTTTCT AAATCTTGTA CCAGATATTG AAGAGATTCG TCCAGGATCT 
GTGGTCTCAA AGAAAGGTTA CTTGAACTTC AAAGAACCTC TGTCTCATTC CTGGGTCAAG 
CATTTTGTGG TTGTGCGCCG TCCCTATGTC TTCATCTACA ACAGTGACAA GGACCCAGTA 
GAGAGGGGCC TCATTAACTT ATCCACTGCA CAAGTGGAGT ACAGTGAAGA CCAGCAAGCC 
ATGGTTAAGA GCTCAAACAT ATTTGCTGTA TGCACAATGT ACCGTGGGAT CCTTCTGCAG 
GCCATTAATG ATAAAGATAT GAATGACTGG TTGTATGCCT TTAATCCTCT GCTTGCTGGC 
ACAATAAGGT CAAAGCTGGC AAGACGTCGT TCTCTTTCCT TGAAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966959.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822413.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X10, mRNA.

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CloneID	OXa32941
Clone ID Related Accession (Same CDS sequence)	XM_012966959.2
Accession Version	XM_012966959.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966959.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGGCT GTTTCTTGAT AAGGATATGC CTAGAACTTT TTATCGCTTT 
GAGGCAGTAT GGGACAGTTC CTTGCATAAC TCCCTGCTTC TAAACCGGGT AACTCCATAT 
GGAGAGAAGA TCTATATGAC CCTATCAGCG TATCTTGAGC TTGATCACTG CATCCAGCCT 
GCAGTTATTA CAAAAGATGT CTGTATGATC TTTTACTCTC GTGATGCCAA AATATCTCCT 
CCACGGTCTC TGCGCAGTCT ATTTGGCAGT GGTTACTCAA AATCCCCTGA CTGTAACAGA 
GTAACTGGAA TATATGAGTT GAGTTTGTGC AAAATGGCTG ATACCGGCAG CCCTGGAATG 
CAACGAAGAA GAAGAAAGAT CCTCGATACG TCAGTGGCAT ATGTTCGTGG TGAAGAGAAT 
CTGGCAGGAT GGAGACCTCG AGGCGATAGT CTAATCCTAG AGCATCAATG GGAACTTGAA 
AAGATGGAGC AATTGCATGA GGTTGAGAAA ACAAGGCACC TCCTTTTACT TAGAGACAAA 
CTAGGAGACA GTATCCCTAA GTCCCTAAGT GAATCTATCT CTCCAAGCTT GAGCAGTGGC 
ACCCTAAGCA CATCGACCAG TATTTCATCG CAGATATCCA CCACAACCTT TGAAAGTGCC 
ATCACACCTA GTGAGAGCAG TGGATATGAC TCTACTGATG TTGAGAGTCT TGTGGATCGT 
GAGAAGGAGT TAGCAACCAA GTGTTTGCGA CTTTTGACTC ACACATTTAA CCAAGAACTA 
ACCCAGGTGT GTAACAGTAT CAGTGACTGT AAGTTGTCTG ATATCTCCCC AATTGGAAGG 
GATCCCTCGG TCTCCAGCTT TAGCAGTGCT ACTCTCACTC CTTCATCAAC TTGCCCATCA 
CTAGCAGATT CTCGATGCAG CTCTGTGGAT CAGAAGACCC CTGAAGCAAA TTCACGTGCC 
TCCAGCCCTT GCACTGAATT TGAACAGTTC CATTTGGTAC CAGCTGTGGA GCGGTCATTT 
TTGGCTCGTG CAGGAAAGAA CGAGTTTCTA AATCTTGTAC CAGATATTGA AGAGATTCGT 
CCAGGATCTG TGGTCTCAAA GAAAGGTTAC TTGAACTTCA AAGAACCTCT GTCTCATTCC 
TGGGTCAAGC ATTTTGTGGT TGTGCGCCGT CCCTATGTCT TCATCTACAA CAGTGACAAG 
GACCCAGTAG AGAGGGGCCT CATTAACTTA TCCACTGCAC AAGTGGAGTA CAGTGAAGAC 
CAGCAAGCCA TGGTTAAGAG CTCAAACATA TTTGCTGTAT GCACAATGTA CCGTGGGATC 
CTTCTGCAGG CCATTAATGA TAAAGATATG AATGACTGGT TGTATGCCTT TAATCCTCTG 
CTTGCTGGCA CAATAAGGTC AAAGCTGGCA AGACGTCGTT CTCTTTCCTT GAAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822413.1
CDS	357..5456
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966959.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966959.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGGCT GTTTCTTGAT AAGGATATGC CTAGAACTTT TTATCGCTTT 
GAGGCAGTAT GGGACAGTTC CTTGCATAAC TCCCTGCTTC TAAACCGGGT AACTCCATAT 
GGAGAGAAGA TCTATATGAC CCTATCAGCG TATCTTGAGC TTGATCACTG CATCCAGCCT 
GCAGTTATTA CAAAAGATGT CTGTATGATC TTTTACTCTC GTGATGCCAA AATATCTCCT 
CCACGGTCTC TGCGCAGTCT ATTTGGCAGT GGTTACTCAA AATCCCCTGA CTGTAACAGA 
GTAACTGGAA TATATGAGTT GAGTTTGTGC AAAATGGCTG ATACCGGCAG CCCTGGAATG 
CAACGAAGAA GAAGAAAGAT CCTCGATACG TCAGTGGCAT ATGTTCGTGG TGAAGAGAAT 
CTGGCAGGAT GGAGACCTCG AGGCGATAGT CTAATCCTAG AGCATCAATG GGAACTTGAA 
AAGATGGAGC AATTGCATGA GGTTGAGAAA ACAAGGCACC TCCTTTTACT TAGAGACAAA 
CTAGGAGACA GTATCCCTAA GTCCCTAAGT GAATCTATCT CTCCAAGCTT GAGCAGTGGC 
ACCCTAAGCA CATCGACCAG TATTTCATCG CAGATATCCA CCACAACCTT TGAAAGTGCC 
ATCACACCTA GTGAGAGCAG TGGATATGAC TCTACTGATG TTGAGAGTCT TGTGGATCGT 
GAGAAGGAGT TAGCAACCAA GTGTTTGCGA CTTTTGACTC ACACATTTAA CCAAGAACTA 
ACCCAGGTGT GTAACAGTAT CAGTGACTGT AAGTTGTCTG ATATCTCCCC AATTGGAAGG 
GATCCCTCGG TCTCCAGCTT TAGCAGTGCT ACTCTCACTC CTTCATCAAC TTGCCCATCA 
CTAGCAGATT CTCGATGCAG CTCTGTGGAT CAGAAGACCC CTGAAGCAAA TTCACGTGCC 
TCCAGCCCTT GCACTGAATT TGAACAGTTC CATTTGGTAC CAGCTGTGGA GCGGTCATTT 
TTGGCTCGTG CAGGAAAGAA CGAGTTTCTA AATCTTGTAC CAGATATTGA AGAGATTCGT 
CCAGGATCTG TGGTCTCAAA GAAAGGTTAC TTGAACTTCA AAGAACCTCT GTCTCATTCC 
TGGGTCAAGC ATTTTGTGGT TGTGCGCCGT CCCTATGTCT TCATCTACAA CAGTGACAAG 
GACCCAGTAG AGAGGGGCCT CATTAACTTA TCCACTGCAC AAGTGGAGTA CAGTGAAGAC 
CAGCAAGCCA TGGTTAAGAG CTCAAACATA TTTGCTGTAT GCACAATGTA CCGTGGGATC 
CTTCTGCAGG CCATTAATGA TAAAGATATG AATGACTGGT TGTATGCCTT TAATCCTCTG 
CTTGCTGGCA CAATAAGGTC AAAGCTGGCA AGACGTCGTT CTCTTTCCTT GAAGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966960.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822414.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X11, mRNA.

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CloneID	OXa32942
Clone ID Related Accession (Same CDS sequence)	XM_012966960.2
Accession Version	XM_012966960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966960.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGAT ATGCAGATCG TGCTAAACAG ATTAAGTGCA ATGCTGTTAT CAATGAAGAT 
CCCAATGCCA AACTTGTGCG TGAGCTAAAA GATGAAGTAA CAAGGCTTAA AGACCTCTTG 
CGTGCTCAGG GATTGGGCGA TATCCTTGAT ACTCTTCCTT CTGTCGGGTG CTTTCCTATC 
TCAGACATGA ATGATAATGA GAACCTTAAG AATAAATACT TATTAGCCAA TGAAAACCAA 
CGACCTGGCA ATTTCTCCAC AGCCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CGGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGGC TGTTTCTTGA TAAGGATATG CCTAGAACTT TTTATCGCTT TGAGGCAGTA 
TGGGACAGTT CCTTGCATAA CTCCCTGCTT CTAAACCGGG TAACTCCATA TGGAGAGAAG 
ATCTATATGA CCCTATCAGC GTATCTTGAG CTTGATCACT GCATCCAGCC TGCAGTTATT 
ACAAAAGATG TCTGTATGAT CTTTTACTCT CGTGATGCCA AAATATCTCC TCCACGGTCT 
CTGCGCAGTC TATTTGGCAG TGGTTACTCA AAATCCCCTG ACTGTAACAG AGTAACTGGA 
ATATATGAGT TGAGTTTGTG CAAAATGGCT GATACCGGCA GCCCTGGAAT GCAACGAAGA 
AGAAGAAAGA TCCTCGATAC GTCAGTGGCA TATGTTCGTG GTGAAGAGAA TCTGGCAGGA 
TGGAGACCTC GAGGCGATAG TCTAATCCTA GAGCATCAAT GGGAACTTGA AAAGATGGAG 
CAATTGCATG AGGTTGAGAA AACAAGGCAC CTCCTTTTAC TTAGAGACAA ACTAGGAGAC 
AGTATCCCTA AGTCCCTAAG TGAATCTATC TCTCCAAGCT TGAGCAGTGG CACCCTAAGC 
ACATCGACCA GTATTTCATC GCAGATATCC ACCACAACCT TTGAAAGTGC CATCACACCT 
AGTGAGAGCA GTGGATATGA CTCTACTGAT GTTGAGAGTC TTGTGGATCG TGAGAAGGAG 
TTAGCAACCA AGTGTTTGCG ACTTTTGACT CACACATTTA ACCAAGAACT AACCCAGGTG 
TGTAACAGTA TCAGTGACTG TAAGTTGTCT GATATCTCCC CAATTGGAAG GGATCCCTCG 
GTCTCCAGCT TTAGCAGTGC TACTCTCACT CCTTCATCAA CTTGCCCATC ACTAGCAGAT 
TCTCGATGCA GCTCTGTGGA TCAGAAGACC CCTGAAGCAA ATTCACGTGC CTCCAGCCCT 
TGCACTGAAT TTGAACAGTT CCATTTGGTA CCAGCTGTGG AGCGGTCATT TTTGGCTCGT 
GCAGGAAAGA ACGAGTTTCT AAATCTTGTA CCAGATATTG AAGAGATTCG TCCAGGATCT 
GTGGTCTCAA AGAAAGGTTA CTTGAACTTC AAAGAACCTC TGTCTCATTC CTGGGTCAAG 
CATTTTGTGG TTGTGCGCCG TCCCTATGTC TTCATCTACA ACAGTGACAA GGACCCAGTA 
GAGAGGGGCC TCATTAACTT ATCCACTGCA CAAGTGGAGT ACAGTGAAGA CCAGCAAGCC 
ATGGTTAAGA GCTCAAACAT ATTTGCTGTA TGCACAATGT ACCGTGGGAT CCTTCTGCAG 
GCCATTAATG ATAAAGATAT GAATGACTGG TTGTATGCCT TTAATCCTCT GCTTGCTGGC 
ACAATAAGGT CAAAGCTGGC AAGACGTCGT TCTCTTTCCT TGAAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822414.1
CDS	78..4508
Translation	MRRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTLPSVGCFPISDMNDNENLKNKYLLANENQRPGNFSTASISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDQMEDFDDEPFVDDTGSDVGAEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRLFLDKDMPRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966960.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966960.2

ORF Insert Sequence:

ATGCGCAGAT ATGCAGATCG TGCTAAACAG ATTAAGTGCA ATGCTGTTAT CAATGAAGAT 
CCCAATGCCA AACTTGTGCG TGAGCTAAAA GATGAAGTAA CAAGGCTTAA AGACCTCTTG 
CGTGCTCAGG GATTGGGCGA TATCCTTGAT ACTCTTCCTT CTGTCGGGTG CTTTCCTATC 
TCAGACATGA ATGATAATGA GAACCTTAAG AATAAATACT TATTAGCCAA TGAAAACCAA 
CGACCTGGCA ATTTCTCCAC AGCCTCCATC AGTTCTCTAG CATCCTCACC ATCCTCCTGT 
TCACTTAGTA GTCAGGTGGG ATTGACATCT GTTACCAGCA TCCAGGAAAG AATCATGTCT 
ACCCCAGGAG GAGAGGAAGC TATAGAGCGA CTTAAAGAGT CTGAAAAAAT AATTGCAGAG 
CTAAATGAGA CATGGGAAGA GAAGCTGCGA AAAACAGAGG CTATTCGAAT GGACAGGGAG 
GCTTTACTTG CAGAAATGGG AGTGGCTATT CGTGAAGATG GTGGAACCTT GGGGGTTTTC 
TCACCCAAAA AGACTCCCCA TCTTGTCAAT CTTAATGAAG ATCCACTAAT GTCTGAATGC 
CTTCTATACT ACATTAAAGA TGGACTTACA AGGGTCGGTC AGGCAGATGC TGATAGTCGC 
CAGGATATTG TGCTAAGTGG AGCTCATATT AAAGAGGAAC ACTGCATATT CCGTAGTGAA 
AGGAATTCAA ATGGGGAAGT AATAGTAACC TTGGAGCCCG GTGACAGGTC AGAAACATAT 
GTCAATGGAA AACGAGTTGT GCATCCAGTG GAGTTAAGAT CTGGAAATCG AATAATAATG 
GGAAAAAACC ATGTATTTCG TTTTAACCAC CCTGAGCAAG CCCGTGCTGA ACGGGAGAAA 
ACGCCTTCTG CAGAAACACC CTCAGAACCA GTGGACTGGA CATTTGCTCA GCGTGAGCTG 
TTGGAGAAAC AAGGCATCGA TATGAAGCAA GAAATGGAGA AAAGGCTTCA GGAGATGGAA 
ATTTTGTACA AGAAAGAAAA GGAAGAGGCA GATCTTCTTT TGGAGCAACA AAGGCTGGAC 
TATGAGAGCA AACTGCAAGC TCTGCAAAAA CAAGTCGAGA CTCACTCACT GGCAGCAGAG 
ACCACAGATG ATGGGGAAGA AGAAGAGGAG GAAGTTCCAT GGACACAGCA TGAATATGAT 
GTAGCACTGT GGGCATTTAA AAAGTGGAGA AATCACCAGT TCACCTCGTT ACGGGACTTG 
CTTTGGGGGA ATGCCATGTA TCTGAAGGAA GCCAATGCAA TAAGTGTAGA GCTGAAGAAG 
AAGGTGCAGT TCCAATTTGT GCTGCTTACA GATACTTTGT ACTCCCCACT GCCACCAGAG 
TTACTTCCTT CTGATGCTGA TAAAACCACA GAAGACAGGA CTTTCCCTCG AACTGTGGTT 
GCAGTTGAGG TGCAAGATTT AAAAAATGGG GCGACTCATT ACTGGTCACT GGAGAAACTG 
AGGTTAAGGT TAGAACAGAT GCGGGAGATG TATGACCGTG CGGGAGAAAT GGCTTCCCAT 
GTGCAAGATG AAAATGACAC AGTGATAACT GGAAATGACC CTTTTTATGA TCGCTTTCAT 
TGGTTCAAGC TAGTTGGGAG TTCTCCCATA TTCCACGGCT GTGTGAATGA GCGGCTGGCA 
GACCGCACCC CGTCCCCTAC CTTCTCCACT GCAGACTCTG ATATCACAGA GTTAGCTGAT 
GAGCAGCAGG ACCAGATGGA AGACTTTGAT GATGAGCCAT TTGTGGATGA CACTGGCTCT 
GATGTTGGTG CAGAAGAAGG ATCCGATCTC TTCAGTGATG GCCATGACCC GTTTTACGAC 
CGGTCCCCAT GGTTCATTTT AGTAGGAAGG GCTTTCGTCT ATCTAAGCAA CCTGCTTTTC 
CCAGTTCCTC TTGTGCATCG AGCAGCTATA GTAAGTGAGA AAGGAGAAGT ACGTGGGTTT 
TTGCGTGTTG CTGTGCAGGC AATATCTGGT GATGAGGAAG CACCAGACTA TGGGTCCGGC 
ATTAGACAAT CGGGAACAGC CAAGATTTCA TTTGATAATG AATACATTAA TAAGAATGAA 
ATCTCTTCTA TGGTTTTGAC TCGTTCCGGA CTGTCACTAG AAGAACTTCG CATTGTGGAA 
GGACAAGGAC AGAACTCTGA CATTACTTCA TTAGAAGAGG TCAATCATTT AGCTGATTTA 
GACCTAAAGT CTGGAAACCT GTTGGATGGA AAGATGATGA TGGAAGGATT CTCAGAGGAT 
GTAGGAGAGC ATCTGAAAAT TGGTAGCATG TTCACTTTCC GTGTGACAGT GCTTCAGGCC 
AGTGGAATTC CTCCAGAATA TGCTGATATC TTCTGCCAAT TTAACTTTTT ACATCGACAT 
GATGAAGCAT TTTCTACAGA GCCACTGAAG AACAGTGGCA GAGGAAATCC TCTTGGTTTC 
TATCATGTTC AGAATATTGC AGTGGAAATT ACAGAATCTT TTATAGAATA CATTAAAAAC 
AACCCAATTG TTTTTGAAGT ATTTGGCCAT TACCAGCAGC ACCCTCTCCA CCTTCAAGGA 
CAAGATCTCA GCCCACCTCA GCAGTGCCGA CGTTACTTTC CTCCTCCAAT GCCACTTTCT 
AAACCAGTTC CAGCCACTAA ATTAAATCCA CTAAGCAAGC AAAGTCTTGG ACAGGTCATC 
AGCAAGTATG ACTTGTTAGT CTGGTTTGAG ATCAGTGAAC TGGAGCCTAC TGGAGAGTAC 
ATTCCAGCTG TCGTAGATCA TTCTGGAGGA CTGCCCTGCC AGGGAACCTT TTTGCTGCAT 
CAGGGAATCC AGCGCAGAAT TACTGTCACC ATTGTCCATG AGAAAGGGAA TGAGTTGCAC 
TGGAAGGATG TGAGAGAATT GGTTGTAGGA CGTGTTCGCA ATAAAGCAGA TGAAGCTGCT 
GCAGATGCTG TTCTTTCACT AAATATCATC TCTGCAAAAT ACCTCAGGTC TTCTCATAGC 
TCTGGCAGGC TGTTTCTTGA TAAGGATATG CCTAGAACTT TTTATCGCTT TGAGGCAGTA 
TGGGACAGTT CCTTGCATAA CTCCCTGCTT CTAAACCGGG TAACTCCATA TGGAGAGAAG 
ATCTATATGA CCCTATCAGC GTATCTTGAG CTTGATCACT GCATCCAGCC TGCAGTTATT 
ACAAAAGATG TCTGTATGAT CTTTTACTCT CGTGATGCCA AAATATCTCC TCCACGGTCT 
CTGCGCAGTC TATTTGGCAG TGGTTACTCA AAATCCCCTG ACTGTAACAG AGTAACTGGA 
ATATATGAGT TGAGTTTGTG CAAAATGGCT GATACCGGCA GCCCTGGAAT GCAACGAAGA 
AGAAGAAAGA TCCTCGATAC GTCAGTGGCA TATGTTCGTG GTGAAGAGAA TCTGGCAGGA 
TGGAGACCTC GAGGCGATAG TCTAATCCTA GAGCATCAAT GGGAACTTGA AAAGATGGAG 
CAATTGCATG AGGTTGAGAA AACAAGGCAC CTCCTTTTAC TTAGAGACAA ACTAGGAGAC 
AGTATCCCTA AGTCCCTAAG TGAATCTATC TCTCCAAGCT TGAGCAGTGG CACCCTAAGC 
ACATCGACCA GTATTTCATC GCAGATATCC ACCACAACCT TTGAAAGTGC CATCACACCT 
AGTGAGAGCA GTGGATATGA CTCTACTGAT GTTGAGAGTC TTGTGGATCG TGAGAAGGAG 
TTAGCAACCA AGTGTTTGCG ACTTTTGACT CACACATTTA ACCAAGAACT AACCCAGGTG 
TGTAACAGTA TCAGTGACTG TAAGTTGTCT GATATCTCCC CAATTGGAAG GGATCCCTCG 
GTCTCCAGCT TTAGCAGTGC TACTCTCACT CCTTCATCAA CTTGCCCATC ACTAGCAGAT 
TCTCGATGCA GCTCTGTGGA TCAGAAGACC CCTGAAGCAA ATTCACGTGC CTCCAGCCCT 
TGCACTGAAT TTGAACAGTT CCATTTGGTA CCAGCTGTGG AGCGGTCATT TTTGGCTCGT 
GCAGGAAAGA ACGAGTTTCT AAATCTTGTA CCAGATATTG AAGAGATTCG TCCAGGATCT 
GTGGTCTCAA AGAAAGGTTA CTTGAACTTC AAAGAACCTC TGTCTCATTC CTGGGTCAAG 
CATTTTGTGG TTGTGCGCCG TCCCTATGTC TTCATCTACA ACAGTGACAA GGACCCAGTA 
GAGAGGGGCC TCATTAACTT ATCCACTGCA CAAGTGGAGT ACAGTGAAGA CCAGCAAGCC 
ATGGTTAAGA GCTCAAACAT ATTTGCTGTA TGCACAATGT ACCGTGGGAT CCTTCTGCAG 
GCCATTAATG ATAAAGATAT GAATGACTGG TTGTATGCCT TTAATCCTCT GCTTGCTGGC 
ACAATAAGGT CAAAGCTGGC AAGACGTCGT TCTCTTTCCT TGAAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kif1b ( XM_012966961.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822415.1 Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X13, mRNA.

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CloneID	OXa32943
Clone ID Related Accession (Same CDS sequence)	XM_012966961.2
Accession Version	XM_012966961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	kinesin-like protein KIF1B isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966961.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822415.1
CDS	356..5428
Translation	MSGASVKVAVRVRPFNSRELSKESKCIIQMQGNSTSIINPKNPKEAAKSFSFDYSYWSHTSQDDPCFASQSRVYNDIGKEMLEHAFEGYNVCIFAYGQTGAGKSYTMMGKQEETQAGIIPQLCEDLFEKINDNNNDEVSFSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKRLDIGTNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVDNCTSKSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNAKLVRELKDEVTRLKDLLRAQGLGDILDTSISSLASSPSSCSLSSQVGLTSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMDREALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGLTRVGQADADSRQDIVLSGAHIKEEHCIFRSERNSNGEVIVTLEPGDRSETYVNGKRVVHPVELRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLDYESKLQALQKQVETHSLAAETTDDGEEEEEEVPWTQHEYDVALWAFKKWRNHQFTSLRDLLWGNAMYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPSDADKTTEDRTFPRTVVAVEVQDLKNGATHYWSLEKLRLRLEQMREMYDRAGEMASHVQDENDTVITGNDPFYDRFHWFKLVGRAFVYLSNLLFPVPLVHRAAIVSEKGEVRGFLRVAVQAISGDEEAPDYGSGIRQSGTAKISFDNEYINKNEISSMVLTRSGLSLEELRIVEGQGQNSDITSLEEVNHLADLDLKSGNLLDGKMMMEGFSEDVGEHLKIGSMFTFRVTVLQASGIPPEYADIFCQFNFLHRHDEAFSTEPLKNSGRGNPLGFYHVQNIAVEITESFIEYIKNNPIVFEVFGHYQQHPLHLQGQDLSPPQQCRRYFPPPMPLSKPVPATKLNPLSKQSLGQVISKYDLLVWFEISELEPTGEYIPAVVDHSGGLPCQGTFLLHQGIQRRITVTIVHEKGNELHWKDVRELVVGRVRNKADEAAADAVLSLNIISAKYLRSSHSSGRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMIFYSRDAKISPPRSLRSLFGSGYSKSPDCNRVTGIYELSLCKMADTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKMEQLHEVEKTRHLLLLRDKLGDSIPKSLSESISPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSTDVESLVDREKELATKCLRLLTHTFNQELTQVCNSISDCKLSDISPIGRDPSVSSFSSATLTPSSTCPSLADSRCSSVDQKTPEANSRASSPCTEFEQFHLVPAVERSFLARAGKNEFLNLVPDIEEIRPGSVVSKKGYLNFKEPLSHSWVKHFVVVRRPYVFIYNSDKDPVERGLINLSTAQVEYSEDQQAMVKSSNIFAVCTMYRGILLQAINDKDMNDWLYAFNPLLAGTIRSKLARRRSLSLKY

Target ORF information:

RefSeq Version	XM_012966961.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis kinesin family member 1B (kif1b), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966961.2

ORF Insert Sequence:

ATGTCGGGGG CTTCAGTGAA AGTTGCTGTC AGAGTCCGTC CCTTTAATTC TCGGGAGCTC 
AGCAAGGAAT CCAAGTGCAT CATTCAGATG CAAGGCAACT CAACCAGTAT TATTAATCCA 
AAGAATCCTA AGGAAGCTGC CAAAAGTTTT AGCTTTGACT ACTCTTATTG GTCTCACACA 
TCGCAAGATG ACCCTTGCTT TGCATCCCAG AGTCGTGTAT ATAATGATAT TGGAAAAGAA 
ATGCTTGAGC ATGCCTTTGA GGGCTACAAT GTTTGCATAT TTGCTTATGG GCAGACAGGA 
GCTGGAAAAT CCTACACTAT GATGGGTAAA CAAGAAGAGA CACAAGCTGG CATCATCCCA 
CAGCTCTGTG AGGATCTTTT CGAAAAAATT AATGACAACA ATAATGATGA AGTTTCTTTT 
TCTGTAGAGG TGAGCTACAT GGAAATATAC TGTGAGCGTG TCAGAGACCT CCTAAACCCA 
AAAAACAAAG GAAACTTGCG TGTTAGGGAG CACCCACTAT TAGGGCCCTA TGTGGAGGAC 
CTTTCCAAAC TGGCTGTCAC CTCTTATACT GATATTGCTG ACTTGATGGA TGCAGGCAAT 
AAAGCTAGGA CTGTAGCCGC TACCAACATG AATGAGACCA GCAGCCGCTC CCATGCAGTA 
TTCACTATTG TGTTTACTCA AAAGAGACTA GATATAGGGA CAAACCTCTC CACAGAAAAG 
GTCAGCAAGA TTAGCTTAGT GGATTTGGCA GGGAGTGAGA GGGCTGATTC AACTGGAGCA 
AAGGGTACTC GTCTAAAAGA AGGAGCTAAT ATCAACAAGT CACTAACAAC CTTGGGAAAA 
GTTATATCTG CTCTTGCAGA GGTGGATAAC TGCACAAGCA AGAGTAAGAA GAAGAAAAAA 
ACAGACTTCA TTCCATATCG AGATTCTGTA CTTACATGGC TGCTTCGTGA GAACTTAGGT 
GGCAATTCAC GGACAGCAAT GGTTGCAGCT CTGAGTCCTG CCGATATAAA CTATGATGAG 
ACTCTAAGTA CATTAAGATA TGCAGATCGT GCTAAACAGA TTAAGTGCAA TGCTGTTATC 
AATGAAGATC CCAATGCCAA ACTTGTGCGT GAGCTAAAAG ATGAAGTAAC AAGGCTTAAA 
GACCTCTTGC GTGCTCAGGG ATTGGGCGAT ATCCTTGATA CCTCCATCAG TTCTCTAGCA 
TCCTCACCAT CCTCCTGTTC ACTTAGTAGT CAGGTGGGAT TGACATCTGT TACCAGCATC 
CAGGAAAGAA TCATGTCTAC CCCAGGAGGA GAGGAAGCTA TAGAGCGACT TAAAGAGTCT 
GAAAAAATAA TTGCAGAGCT AAATGAGACA TGGGAAGAGA AGCTGCGAAA AACAGAGGCT 
ATTCGAATGG ACAGGGAGGC TTTACTTGCA GAAATGGGAG TGGCTATTCG TGAAGATGGT 
GGAACCTTGG GGGTTTTCTC ACCCAAAAAG ACTCCCCATC TTGTCAATCT TAATGAAGAT 
CCACTAATGT CTGAATGCCT TCTATACTAC ATTAAAGATG GACTTACAAG GGTCGGTCAG 
GCAGATGCTG ATAGTCGCCA GGATATTGTG CTAAGTGGAG CTCATATTAA AGAGGAACAC 
TGCATATTCC GTAGTGAAAG GAATTCAAAT GGGGAAGTAA TAGTAACCTT GGAGCCCGGT 
GACAGGTCAG AAACATATGT CAATGGAAAA CGAGTTGTGC ATCCAGTGGA GTTAAGATCT 
GGAAATCGAA TAATAATGGG AAAAAACCAT GTATTTCGTT TTAACCACCC TGAGCAAGCC 
CGTGCTGAAC GGGAGAAAAC GCCTTCTGCA GAAACACCCT CAGAACCAGT GGACTGGACA 
TTTGCTCAGC GTGAGCTGTT GGAGAAACAA GGCATCGATA TGAAGCAAGA AATGGAGAAA 
AGGCTTCAGG AGATGGAAAT TTTGTACAAG AAAGAAAAGG AAGAGGCAGA TCTTCTTTTG 
GAGCAACAAA GGCTGGACTA TGAGAGCAAA CTGCAAGCTC TGCAAAAACA AGTCGAGACT 
CACTCACTGG CAGCAGAGAC CACAGATGAT GGGGAAGAAG AAGAGGAGGA AGTTCCATGG 
ACACAGCATG AATATGATGT AGCACTGTGG GCATTTAAAA AGTGGAGAAA TCACCAGTTC 
ACCTCGTTAC GGGACTTGCT TTGGGGGAAT GCCATGTATC TGAAGGAAGC CAATGCAATA 
AGTGTAGAGC TGAAGAAGAA GGTGCAGTTC CAATTTGTGC TGCTTACAGA TACTTTGTAC 
TCCCCACTGC CACCAGAGTT ACTTCCTTCT GATGCTGATA AAACCACAGA AGACAGGACT 
TTCCCTCGAA CTGTGGTTGC AGTTGAGGTG CAAGATTTAA AAAATGGGGC GACTCATTAC 
TGGTCACTGG AGAAACTGAG GTTAAGGTTA GAACAGATGC GGGAGATGTA TGACCGTGCG 
GGAGAAATGG CTTCCCATGT GCAAGATGAA AATGACACAG TGATAACTGG AAATGACCCT 
TTTTATGATC GCTTTCATTG GTTCAAGCTA GTTGGGAGGG CTTTCGTCTA TCTAAGCAAC 
CTGCTTTTCC CAGTTCCTCT TGTGCATCGA GCAGCTATAG TAAGTGAGAA AGGAGAAGTA 
CGTGGGTTTT TGCGTGTTGC TGTGCAGGCA ATATCTGGTG ATGAGGAAGC ACCAGACTAT 
GGGTCCGGCA TTAGACAATC GGGAACAGCC AAGATTTCAT TTGATAATGA ATACATTAAT 
AAGAATGAAA TCTCTTCTAT GGTTTTGACT CGTTCCGGAC TGTCACTAGA AGAACTTCGC 
ATTGTGGAAG GACAAGGACA GAACTCTGAC ATTACTTCAT TAGAAGAGGT CAATCATTTA 
GCTGATTTAG ACCTAAAGTC TGGAAACCTG TTGGATGGAA AGATGATGAT GGAAGGATTC 
TCAGAGGATG TAGGAGAGCA TCTGAAAATT GGTAGCATGT TCACTTTCCG TGTGACAGTG 
CTTCAGGCCA GTGGAATTCC TCCAGAATAT GCTGATATCT TCTGCCAATT TAACTTTTTA 
CATCGACATG ATGAAGCATT TTCTACAGAG CCACTGAAGA ACAGTGGCAG AGGAAATCCT 
CTTGGTTTCT ATCATGTTCA GAATATTGCA GTGGAAATTA CAGAATCTTT TATAGAATAC 
ATTAAAAACA ACCCAATTGT TTTTGAAGTA TTTGGCCATT ACCAGCAGCA CCCTCTCCAC 
CTTCAAGGAC AAGATCTCAG CCCACCTCAG CAGTGCCGAC GTTACTTTCC TCCTCCAATG 
CCACTTTCTA AACCAGTTCC AGCCACTAAA TTAAATCCAC TAAGCAAGCA AAGTCTTGGA 
CAGGTCATCA GCAAGTATGA CTTGTTAGTC TGGTTTGAGA TCAGTGAACT GGAGCCTACT 
GGAGAGTACA TTCCAGCTGT CGTAGATCAT TCTGGAGGAC TGCCCTGCCA GGGAACCTTT 
TTGCTGCATC AGGGAATCCA GCGCAGAATT ACTGTCACCA TTGTCCATGA GAAAGGGAAT 
GAGTTGCACT GGAAGGATGT GAGAGAATTG GTTGTAGGAC GTGTTCGCAA TAAAGCAGAT 
GAAGCTGCTG CAGATGCTGT TCTTTCACTA AATATCATCT CTGCAAAATA CCTCAGGTCT 
TCTCATAGCT CTGGCAGAAC TTTTTATCGC TTTGAGGCAG TATGGGACAG TTCCTTGCAT 
AACTCCCTGC TTCTAAACCG GGTAACTCCA TATGGAGAGA AGATCTATAT GACCCTATCA 
GCGTATCTTG AGCTTGATCA CTGCATCCAG CCTGCAGTTA TTACAAAAGA TGTCTGTATG 
ATCTTTTACT CTCGTGATGC CAAAATATCT CCTCCACGGT CTCTGCGCAG TCTATTTGGC 
AGTGGTTACT CAAAATCCCC TGACTGTAAC AGAGTAACTG GAATATATGA GTTGAGTTTG 
TGCAAAATGG CTGATACCGG CAGCCCTGGA ATGCAACGAA GAAGAAGAAA GATCCTCGAT 
ACGTCAGTGG CATATGTTCG TGGTGAAGAG AATCTGGCAG GATGGAGACC TCGAGGCGAT 
AGTCTAATCC TAGAGCATCA ATGGGAACTT GAAAAGATGG AGCAATTGCA TGAGGTTGAG 
AAAACAAGGC ACCTCCTTTT ACTTAGAGAC AAACTAGGAG ACAGTATCCC TAAGTCCCTA 
AGTGAATCTA TCTCTCCAAG CTTGAGCAGT GGCACCCTAA GCACATCGAC CAGTATTTCA 
TCGCAGATAT CCACCACAAC CTTTGAAAGT GCCATCACAC CTAGTGAGAG CAGTGGATAT 
GACTCTACTG ATGTTGAGAG TCTTGTGGAT CGTGAGAAGG AGTTAGCAAC CAAGTGTTTG 
CGACTTTTGA CTCACACATT TAACCAAGAA CTAACCCAGG TGTGTAACAG TATCAGTGAC 
TGTAAGTTGT CTGATATCTC CCCAATTGGA AGGGATCCCT CGGTCTCCAG CTTTAGCAGT 
GCTACTCTCA CTCCTTCATC AACTTGCCCA TCACTAGCAG ATTCTCGATG CAGCTCTGTG 
GATCAGAAGA CCCCTGAAGC AAATTCACGT GCCTCCAGCC CTTGCACTGA ATTTGAACAG 
TTCCATTTGG TACCAGCTGT GGAGCGGTCA TTTTTGGCTC GTGCAGGAAA GAACGAGTTT 
CTAAATCTTG TACCAGATAT TGAAGAGATT CGTCCAGGAT CTGTGGTCTC AAAGAAAGGT 
TACTTGAACT TCAAAGAACC TCTGTCTCAT TCCTGGGTCA AGCATTTTGT GGTTGTGCGC 
CGTCCCTATG TCTTCATCTA CAACAGTGAC AAGGACCCAG TAGAGAGGGG CCTCATTAAC 
TTATCCACTG CACAAGTGGA GTACAGTGAA GACCAGCAAG CCATGGTTAA GAGCTCAAAC 
ATATTTGCTG TATGCACAAT GTACCGTGGG ATCCTTCTGC AGGCCATTAA TGATAAAGAT 
ATGAATGACT GGTTGTATGC CTTTAATCCT CTGCTTGCTG GCACAATAAG GTCAAAGCTG 
GCAAGACGTC GTTCTCTTTC CTTGAAGTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kif1b
Entrez Gene ID	100379810
Full Name	kinesin family member 1B
General protein information	Preferred Names kinesin family member 1B Names kinesin-like protein KIF1B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	LOC722674	XP_002808317.1
Gallus gallus (chicken)	KIF1B	XP_004947511.1
Rattus norvegicus (Norway rat)	Kif1b	NP_476548.1
Canis lupus familiaris (dog)	KIF1B	XP_536743.3
Mus musculus (house mouse)	Kif1b	NP_997565.2
Danio rerio (zebrafish)	kif1b	NP_919363.2
Homo sapiens (human)	KIF1B	NP_055889.2
Bos taurus (cattle)	KIF1B	XP_005217049.1
Xenopus tropicalis (tropical clawed frog)	kif1b	XP_002939603.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kif1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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