cast gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012956118.1	XP_012811572.1	calpastatin isoform X18
XM_031895384.1	XP_031751244.1	calpastatin isoform X5
XM_031895388.1	XP_031751248.1	calpastatin isoform X6
XM_031895403.1	XP_031751263.1	calpastatin isoform X11
XM_031895421.1	XP_031751281.1	calpastatin isoform X16
XM_012956032.1	XP_012811486.1	calpastatin isoform X2
XM_012956046.2	XP_012811500.1	calpastatin isoform X4
XM_012956066.1	XP_012811520.1	calpastatin isoform X8
XM_012956101.1	XP_012811555.1	calpastatin isoform X13
XM_018090967.1	XP_017946456.1	calpastatin isoform X16
XM_012956133.1	XP_012811587.1	calpastatin isoform X20
XM_031895377.1	XP_031751237.1	calpastatin isoform X2
XM_031895380.1	XP_031751240.1	calpastatin isoform X3
XM_031895407.1	XP_031751267.1	calpastatin isoform X12
XM_031895413.1	XP_031751273.1	calpastatin isoform X14
XM_012956026.1	XP_012811480.1	calpastatin isoform X1
XM_012956081.1	XP_012811535.1	calpastatin isoform X10
XM_018090966.1	XP_017946455.1	calpastatin isoform X14
XM_012956125.1	XP_012811579.1	calpastatin isoform X19
NM_001126837.1	NP_001120309.1	calpastatin
XM_031895362.1	XP_031751222.1	calpastatin isoform X1
XM_031895367.1	XP_031751227.1	calpastatin isoform X1
XM_031895397.1	XP_031751257.1	calpastatin isoform X7
XM_012956074.2	XP_012811528.1	calpastatin isoform X9
XM_012956081.2	XP_012811535.1	calpastatin isoform X10
XM_012956113.2	XP_012811567.1	calpastatin isoform X15
XM_031895424.1	XP_031751284.1	calpastatin isoform X17
XM_012956023.1	XP_012811477.1	calpastatin isoform X1
XM_012956040.1	XP_012811494.1	calpastatin isoform X3
XM_018090942.1	XP_017946431.1	calpastatin isoform X5
XM_012956054.1	XP_012811508.1	calpastatin isoform X6
XM_012956093.1	XP_012811547.1	calpastatin isoform X12
XM_012956106.2	XP_012811560.1	calpastatin isoform X15
XM_012956113.1	XP_012811567.1	calpastatin isoform X17
XM_031895374.1	XP_031751234.1	calpastatin isoform X1
XM_031895399.1	XP_031751259.1	calpastatin isoform X8
XM_031895410.1	XP_031751270.1	calpastatin isoform X13
XM_012956017.1	XP_012811471.1	calpastatin isoform X1
XM_018090946.1	XP_017946435.1	calpastatin isoform X7
XM_012956074.1	XP_012811528.1	calpastatin isoform X9
XM_012956088.2	XP_012811542.1	calpastatin isoform X11
XM_031895373.1	XP_031751233.1	calpastatin isoform X1
XM_012956017.2	XP_012811471.1	calpastatin isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa31736	XM_012956101.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
OXa31732	XM_012956074.2 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa31733	XM_012956081.2 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa31738	XM_012956113.2 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47520	XM_031895384.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47521	XM_031895388.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47522	XM_031895397.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47523	XM_031895399.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47524	XM_031895403.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47525	XM_031895407.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47526	XM_031895410.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47527	XM_031895413.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47528	XM_031895421.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa47529	XM_031895424.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa31725	XM_012956017.2 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47517	XM_031895367.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47517	XM_031895362.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47517	XM_031895374.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47517	XM_031895373.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47518	XM_031895377.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa47519	XM_031895380.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa21874	NM_001126837.1 Latest version!	Xenopus tropicalis calpastatin (cast), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31725	XM_012956023.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31725	XM_012956026.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31726	XM_012956032.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31727	XM_012956040.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OXa31728	XM_012956046.2 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31729	XM_012956054.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31731	XM_012956066.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31734	XM_012956088.2 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31735	XM_012956093.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31737	XM_012956106.2 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31739	XM_012956118.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31740	XM_012956125.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31741	XM_012956133.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa41680	XM_018090942.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa41681	XM_018090946.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa41682	XM_018090966.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa41683	XM_018090967.1 Latest version!	Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OXa31733	XM_012956081.1	Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31738	XM_012956113.1	Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$510.30 $729.00
OXa31732	XM_012956074.1	Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OXa31725	XM_012956017.1	Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

cast ( XM_012956101.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811555.1 Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OXa31736
Clone ID Related Accession (Same CDS sequence)	XM_012956101.1
Accession Version	XM_012956101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT 
CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA 
GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG 
CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT 
CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG 
GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA 
CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG 
ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA 
ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA 
GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA 
GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA 
CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA 
GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG 
GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG 
GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC 
CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT 
AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC 
GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA 
AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG 
GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT 
AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT 
GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT 
GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG 
TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT 
GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811555.1
CDS	206..3109
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956101.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956101.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT 
CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA 
GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG 
CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT 
CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG 
GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA 
CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG 
ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA 
ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA 
GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA 
GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA 
CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA 
GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG 
GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG 
GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC 
CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT 
AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC 
GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA 
AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG 
GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT 
AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT 
GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT 
GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG 
TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT 
GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956113.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811567.1 Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.

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CloneID	OXa31738
Clone ID Related Accession (Same CDS sequence)	XM_012956113.2 , XM_012956113.1
Accession Version	XM_012956113.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT 
AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC 
TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA 
ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA 
TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA 
GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC 
GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA 
CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA 
GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA 
CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG 
AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA 
AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT 
AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811567.1
CDS	206..3046
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956113.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956113.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT 
AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC 
TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA 
ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA 
TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA 
GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC 
GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA 
CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA 
GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA 
CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG 
AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA 
AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT 
AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956074.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811528.1 Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa31732
Clone ID Related Accession (Same CDS sequence)	XM_012956074.2 , XM_012956074.1
Accession Version	XM_012956074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811528.1
CDS	205..3150
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956074.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956074.2

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956081.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811535.1 Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OXa31733
Clone ID Related Accession (Same CDS sequence)	XM_012956081.1 , XM_012956081.2
Accession Version	XM_012956081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811535.1
CDS	205..3147
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956081.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956081.2

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956113.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811567.1 Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OXa31738
Clone ID Related Accession (Same CDS sequence)	XM_012956113.2 , XM_012956113.1
Accession Version	XM_012956113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT 
AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC 
TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA 
ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA 
TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA 
GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC 
GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA 
CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA 
GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA 
CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG 
AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA 
AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT 
AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811567.1
CDS	205..3045
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956113.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956113.2

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAGAAAC TCCTCAAGGT 
AACAAAACGA AACCTGAAAT TGCGGCATCA TCTGCATCCA CTGTAAAAGC AGCAGCGCCC 
TCCGGTGGTA TGGACAGTGC ATTAGATGAA TTATTGGGAA CACTGGAAGG TCCTGATCCA 
ACAGCCCCTG AATCACCTGT GTACACTGGG CCAGAGGTGA CGGAAACAGA GACAGCGGTA 
TACAGAGAGG GACTTGGGAT AAGAGACAGC AGCATCCCTC CAGCATACAG GCACTTACTA 
GATGGGAAAG TAGATGGAAA AGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC 
GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA 
CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA 
GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA 
CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG 
AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA 
AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT 
AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895384.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751244.1 Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa47520
Clone ID Related Accession (Same CDS sequence)	XM_031895384.1
Accession Version	XM_031895384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT 
GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT 
AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA 
ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA 
GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC 
AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT 
GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT 
GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA 
GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT 
ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC 
GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA 
CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT 
TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC 
CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG 
TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG 
AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA 
AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA 
AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA 
GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA 
GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC 
GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG 
GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT 
AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG 
TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT 
TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC 
AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA 
TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC 
ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA 
ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG 
ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT 
CCAGCATACA GGCACTTACT AGATGATTCT GCTGGAGGAG CACTATTAAC TGATGCACCC 
CCACCACCGC CAGAGGAAAA GCCTCTGACC GATGATCAGC TGATTGATAA GTTTTCTATG 
GACTTTGATT GTTCTGAATC TCCTGCAACA CAACAGGCTC ATCCTGTCGA ACCTAAAGTT 
TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA GTAGTTGCTG CCTCCTCTGC TACTGTTAAA 
GCTGCACCCA CTCCTTCTAA AGCACCAGCA CCTCCAGCTG ATCCACTCGA TAGCCTGTCT 
GGTACCTTAG GTGTTCGAAA GGAAGAACCG AGTGACAAGA AGCCTCCAGT GGATAAAGTA 
AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA AAGCTTGGAG AAGATGAAAA AACAATACAT 
CCTGACCGCC GGCTAAAGGA AGTGAAGGAT AAAGACGGAA AGCCCTTGCT GCCAAAACTT 
GAACAAAGCC CTAAGCCAAT GAGTGAGGCC GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT 
GTGACTTCCC CTGCCACTCC TGATGCCCCA CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT 
GGATCAGAAG ATGTTATATC TTGTTCTAAA GCTTCCGCCG TCAAGTCTGG AGCTCCACAG 
GACGCTGCTT TAGATATACA GATTCCCGAC GATGCACTAG ATTTACTTTC TGGCTCCCTG 
GGACAAAGAG AGGTGGATCC TAATGAAAAC AAACCGGTGG TGGATGTAGT TAAGGAAAAA 
GCTAAAGCTG AGCATATAGA CAAACTGGGG GACAGAGATG ATACAATCCC ACCGGAATAC 
AGAAAGCTGC TGGATGGTAA AGATGATAAG GGACAACCTG CAAAACCACC AGCGAAAGCG 
GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC GATGCAGCGA TCGATGCTCT TTCAAGCGGA 
TTTGCATCAT GTGACACAAA ATCTACTGAA CAACCAAAGC AGCCTTCAAC GGACAAGACT 
GAGAAACAAT GTGCTAGCGC TCCAGTGTCG CAGCCTTCAG GAAAGGCACC TGATGCTGCA 
AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751244.1
CDS	152..3142
Translation	MAFASWWYATQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895384.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895384.1

ORF Insert Sequence:

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT 
GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT 
AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA 
ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA 
GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC 
AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT 
GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT 
GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA 
GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT 
ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC 
GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA 
CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT 
TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC 
CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG 
TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG 
AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA 
AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA 
AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA 
GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA 
GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC 
GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG 
GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT 
AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG 
TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT 
TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC 
AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA 
TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC 
ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA 
ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG 
ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT 
CCAGCATACA GGCACTTACT AGATGATTCT GCTGGAGGAG CACTATTAAC TGATGCACCC 
CCACCACCGC CAGAGGAAAA GCCTCTGACC GATGATCAGC TGATTGATAA GTTTTCTATG 
GACTTTGATT GTTCTGAATC TCCTGCAACA CAACAGGCTC ATCCTGTCGA ACCTAAAGTT 
TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA GTAGTTGCTG CCTCCTCTGC TACTGTTAAA 
GCTGCACCCA CTCCTTCTAA AGCACCAGCA CCTCCAGCTG ATCCACTCGA TAGCCTGTCT 
GGTACCTTAG GTGTTCGAAA GGAAGAACCG AGTGACAAGA AGCCTCCAGT GGATAAAGTA 
AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA AAGCTTGGAG AAGATGAAAA AACAATACAT 
CCTGACCGCC GGCTAAAGGA AGTGAAGGAT AAAGACGGAA AGCCCTTGCT GCCAAAACTT 
GAACAAAGCC CTAAGCCAAT GAGTGAGGCC GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT 
GTGACTTCCC CTGCCACTCC TGATGCCCCA CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT 
GGATCAGAAG ATGTTATATC TTGTTCTAAA GCTTCCGCCG TCAAGTCTGG AGCTCCACAG 
GACGCTGCTT TAGATATACA GATTCCCGAC GATGCACTAG ATTTACTTTC TGGCTCCCTG 
GGACAAAGAG AGGTGGATCC TAATGAAAAC AAACCGGTGG TGGATGTAGT TAAGGAAAAA 
GCTAAAGCTG AGCATATAGA CAAACTGGGG GACAGAGATG ATACAATCCC ACCGGAATAC 
AGAAAGCTGC TGGATGGTAA AGATGATAAG GGACAACCTG CAAAACCACC AGCGAAAGCG 
GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC GATGCAGCGA TCGATGCTCT TTCAAGCGGA 
TTTGCATCAT GTGACACAAA ATCTACTGAA CAACCAAAGC AGCCTTCAAC GGACAAGACT 
GAGAAACAAT GTGCTAGCGC TCCAGTGTCG CAGCCTTCAG GAAAGGCACC TGATGCTGCA 
AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895388.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751248.1 Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa47521
Clone ID Related Accession (Same CDS sequence)	XM_031895388.1
Accession Version	XM_031895388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA 
AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA 
CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA 
CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT 
ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA 
AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA 
CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT 
TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC 
GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC 
AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC 
GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA 
GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC 
AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT 
GATTCTGCTG GAGGAGCACT ATTAACTGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT 
CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT 
GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA 
AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA 
CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA 
GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG 
AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG 
AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT 
GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT 
GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT 
TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT 
CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT 
GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA 
CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT 
GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA 
AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT 
ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA 
GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA 
AGTGTGCCAA AGGCTGGCAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751248.1
CDS	205..3171
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895388.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895388.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA 
AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA 
CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA 
CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT 
ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA 
AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA 
CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT 
TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC 
GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC 
AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC 
GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA 
GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC 
AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT 
GATTCTGCTG GAGGAGCACT ATTAACTGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT 
CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT 
GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA 
AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA 
CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA 
GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG 
AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG 
AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT 
GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT 
GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT 
TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT 
CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT 
GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA 
CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT 
GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA 
AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT 
ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA 
GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA 
AGTGTGCCAA AGGCTGGCAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895397.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751257.1 Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa47522
Clone ID Related Accession (Same CDS sequence)	XM_031895397.1
Accession Version	XM_031895397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG 
GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA 
AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG 
AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT 
CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT 
GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG 
AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA 
CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA 
GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA 
TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC 
CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG 
CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA 
TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA 
GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA 
ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC 
ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA 
AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC 
CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA 
GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA 
TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG 
TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA 
CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG 
TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA 
ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT 
GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC 
CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA 
GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGAT 
TCTGCTGGAG GAGCACTATT AACTGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG 
ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA 
ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA 
GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA 
GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA 
CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA 
GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG 
GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG 
GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC 
CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT 
AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC 
GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA 
AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG 
GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT 
AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT 
GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT 
GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG 
TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT 
GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751257.1
CDS	205..3168
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895397.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895397.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG 
GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA 
AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG 
AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT 
CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT 
GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG 
AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA 
CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA 
GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA 
TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC 
CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG 
CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA 
TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA 
GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA 
ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC 
ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA 
AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC 
CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA 
GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA 
TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG 
TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA 
CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG 
TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA 
ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT 
GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC 
CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA 
GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGAT 
TCTGCTGGAG GAGCACTATT AACTGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG 
ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA 
ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA 
GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA 
GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA 
CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA 
GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG 
GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG 
GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC 
CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT 
AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC 
GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA 
AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG 
GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT 
AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT 
GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT 
GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG 
TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT 
GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895399.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751259.1 Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa47523
Clone ID Related Accession (Same CDS sequence)	XM_031895399.1
Accession Version	XM_031895399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA 
GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT 
CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG 
GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT 
GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA 
GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC 
AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT 
GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC 
GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG 
CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG 
GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC 
GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA 
CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG 
GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA 
GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA 
CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA 
GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA 
AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT 
TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751259.1
CDS	205..3159
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895399.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895399.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA 
GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT 
CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG 
GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT 
GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA 
GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC 
AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT 
GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC 
GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG 
CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG 
GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC 
GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA 
CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG 
GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA 
GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA 
CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA 
GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA 
AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT 
TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895403.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751263.1 Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa47524
Clone ID Related Accession (Same CDS sequence)	XM_031895403.1
Accession Version	XM_031895403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA 
CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA 
GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT 
TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG 
CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT 
AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT 
AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC 
GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA 
GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC 
TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT 
ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA 
CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG 
GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA 
GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG 
GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG 
CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA 
GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA 
CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA 
GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA 
ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA 
TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA 
AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA 
GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT 
GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT 
AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA 
CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT 
GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA 
TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA 
GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC 
ATCCCTCCAG CATACAGGCA CTTACTAGAT GATTCTGCTG GAGGAGCACT ATTAACTGAT 
GCACCCCCAC CACCGCCAGA GGAAAAGCCT CTGACCGATG ATCAGCTGAT TGATAAGTTT 
TCTATGGACT TTGATTGTTC TGAATCTCCT GCAACACAAC AGGCTCATCC TGTCGAACCT 
AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA AAAGAAGTAG TTGCTGCCTC CTCTGCTACT 
GTTAAAGCTG CACCCACTCC TTCTAAAGCA CCAGCACCTC CAGCTGATCC ACTCGATAGC 
CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA GAACCGAGTG ACAAGAAGCC TCCAGTGGAT 
AAAGTAAAGG AGGGAACAGG CAAAGAGAAG AAAGAAAAGC TTGGAGAAGA TGAAAAAACA 
ATACATCCTG ACCGCCGGCT AAAGGAAGTG AAGGATAAAG ACGGAAAGCC CTTGCTGCCA 
AAACTTGAAC AAAGCCCTAA GCCAATGAGT GAGGCCGAGC TGCTGGATGC TTTGACTGAA 
GGCTTTGTGA CTTCCCCTGC CACTCCTGAT GCCCCACTGC AGGGCTCTTT GAAAAAAAAA 
GGTTCTGGAT CAGAAGATGT TATATCTTGT TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT 
CCACAGGACG CTGCTTTAGA TATACAGATT CCCGACGATG CACTAGATTT ACTTTCTGGC 
TCCCTGGGAC AAAGAGAGGT GGATCCTAAT GAAAACAAAC CGGTGGTGGA TGTAGTTAAG 
GAAAAAGCTA AAGCTGAGCA TATAGACAAA CTGGGGGACA GAGATGATAC AATCCCACCG 
GAATACAGAA AGCTGCTGGA TGGTAAAGAT GATAAGGGAC AACCTGCAAA ACCACCAGCG 
AAAGCGGAGG AGAAACCAAA GAAGCCTTTA AGTGACGATG CAGCGATCGA TGCTCTTTCA 
AGCGGATTTG CATCATGTGA CACAAAATCT ACTGAACAAC CAAAGCAGCC TTCAACGGAC 
AAGACTGAGA AACAATGTGC TAGCGCTCCA GTGTCGCAGC CTTCAGGAAA GGCACCTGAT 
GCTGCAAAGC AGGCAAACTC AGCGATACCA AGTGTGCCAA AGGCTGGCAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751263.1
CDS	146..3082
Translation	MAFASWWYATQCDRKPADKKPSEGAETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895403.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895403.1

ORF Insert Sequence:

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA 
CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA 
GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT 
TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG 
CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT 
AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT 
AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC 
GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA 
GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC 
TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT 
ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA 
CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG 
GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA 
GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG 
GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG 
CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA 
GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA 
CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA 
GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA 
ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA 
TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA 
AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA 
GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT 
GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT 
AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA 
CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT 
GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA 
TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA 
GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC 
ATCCCTCCAG CATACAGGCA CTTACTAGAT GATTCTGCTG GAGGAGCACT ATTAACTGAT 
GCACCCCCAC CACCGCCAGA GGAAAAGCCT CTGACCGATG ATCAGCTGAT TGATAAGTTT 
TCTATGGACT TTGATTGTTC TGAATCTCCT GCAACACAAC AGGCTCATCC TGTCGAACCT 
AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA AAAGAAGTAG TTGCTGCCTC CTCTGCTACT 
GTTAAAGCTG CACCCACTCC TTCTAAAGCA CCAGCACCTC CAGCTGATCC ACTCGATAGC 
CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA GAACCGAGTG ACAAGAAGCC TCCAGTGGAT 
AAAGTAAAGG AGGGAACAGG CAAAGAGAAG AAAGAAAAGC TTGGAGAAGA TGAAAAAACA 
ATACATCCTG ACCGCCGGCT AAAGGAAGTG AAGGATAAAG ACGGAAAGCC CTTGCTGCCA 
AAACTTGAAC AAAGCCCTAA GCCAATGAGT GAGGCCGAGC TGCTGGATGC TTTGACTGAA 
GGCTTTGTGA CTTCCCCTGC CACTCCTGAT GCCCCACTGC AGGGCTCTTT GAAAAAAAAA 
GGTTCTGGAT CAGAAGATGT TATATCTTGT TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT 
CCACAGGACG CTGCTTTAGA TATACAGATT CCCGACGATG CACTAGATTT ACTTTCTGGC 
TCCCTGGGAC AAAGAGAGGT GGATCCTAAT GAAAACAAAC CGGTGGTGGA TGTAGTTAAG 
GAAAAAGCTA AAGCTGAGCA TATAGACAAA CTGGGGGACA GAGATGATAC AATCCCACCG 
GAATACAGAA AGCTGCTGGA TGGTAAAGAT GATAAGGGAC AACCTGCAAA ACCACCAGCG 
AAAGCGGAGG AGAAACCAAA GAAGCCTTTA AGTGACGATG CAGCGATCGA TGCTCTTTCA 
AGCGGATTTG CATCATGTGA CACAAAATCT ACTGAACAAC CAAAGCAGCC TTCAACGGAC 
AAGACTGAGA AACAATGTGC TAGCGCTCCA GTGTCGCAGC CTTCAGGAAA GGCACCTGAT 
GCTGCAAAGC AGGCAAACTC AGCGATACCA AGTGTGCCAA AGGCTGGCAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895407.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751267.1 Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OXa47525
Clone ID Related Accession (Same CDS sequence)	XM_031895407.1
Accession Version	XM_031895407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG 
ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA 
GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA 
ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG 
CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT 
GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT 
GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG 
TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT 
TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG 
TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC 
ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT 
GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG 
GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG 
AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA 
CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC 
GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA 
GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA 
TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA 
GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA 
CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT 
GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA 
GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT 
GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG 
CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA 
GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT 
CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA 
GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT 
GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA 
GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC 
TTACTAGATG ATTCTGCTGG AGGAGCACTA TTAACTGATG CACCCCCACC ACCGCCAGAG 
GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT 
GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA 
AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT 
TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT 
CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC 
AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA 
AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG 
CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC 
ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT 
ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT 
ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG 
GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT 
ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT 
GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG 
AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC 
ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT 
AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA 
GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751267.1
CDS	205..3120
Translation	MSKPGKGRKHSGPGKGTKETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895407.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895407.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG 
ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA 
GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA 
ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG 
CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT 
GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT 
GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG 
TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT 
TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG 
TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC 
ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT 
GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG 
GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG 
AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA 
CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC 
GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA 
GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA 
TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA 
GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA 
CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT 
GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA 
GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT 
GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG 
CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA 
GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT 
CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA 
GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT 
GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA 
GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC 
TTACTAGATG ATTCTGCTGG AGGAGCACTA TTAACTGATG CACCCCCACC ACCGCCAGAG 
GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT 
GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA 
AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT 
TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT 
CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC 
AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA 
AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG 
CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC 
ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT 
ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT 
ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG 
GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT 
ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT 
GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG 
AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC 
ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT 
AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA 
GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895410.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751270.1 Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OXa47526
Clone ID Related Accession (Same CDS sequence)	XM_031895410.1
Accession Version	XM_031895410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT 
CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA 
GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG 
CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT 
CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG 
GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA 
CTAGATGATT CTGCTGGAGG AGCACTATTA ACTGATGCAC CCCCACCACC GCCAGAGGAA 
AAGCCTCTGA CCGATGATCA GCTGATTGAT AAGTTTTCTA TGGACTTTGA TTGTTCTGAA 
TCTCCTGCAA CACAACAGGC TCATCCTGTC GAACCTAAAG TTTCCTCGCA AGAGAAAAAG 
GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT GCTACTGTTA AAGCTGCACC CACTCCTTCT 
AAAGCACCAG CACCTCCAGC TGATCCACTC GATAGCCTGT CTGGTACCTT AGGTGTTCGA 
AAGGAAGAAC CGAGTGACAA GAAGCCTCCA GTGGATAAAG TAAAGGAGGG AACAGGCAAA 
GAGAAGAAAG AAAAGCTTGG AGAAGATGAA AAAACAATAC ATCCTGACCG CCGGCTAAAG 
GAAGTGAAGG ATAAAGACGG AAAGCCCTTG CTGCCAAAAC TTGAACAAAG CCCTAAGCCA 
ATGAGTGAGG CCGAGCTGCT GGATGCTTTG ACTGAAGGCT TTGTGACTTC CCCTGCCACT 
CCTGATGCCC CACTGCAGGG CTCTTTGAAA AAAAAAGGTT CTGGATCAGA AGATGTTATA 
TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT GGAGCTCCAC AGGACGCTGC TTTAGATATA 
CAGATTCCCG ACGATGCACT AGATTTACTT TCTGGCTCCC TGGGACAAAG AGAGGTGGAT 
CCTAATGAAA ACAAACCGGT GGTGGATGTA GTTAAGGAAA AAGCTAAAGC TGAGCATATA 
GACAAACTGG GGGACAGAGA TGATACAATC CCACCGGAAT ACAGAAAGCT GCTGGATGGT 
AAAGATGATA AGGGACAACC TGCAAAACCA CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG 
CCTTTAAGTG ACGATGCAGC GATCGATGCT CTTTCAAGCG GATTTGCATC ATGTGACACA 
AAATCTACTG AACAACCAAA GCAGCCTTCA ACGGACAAGA CTGAGAAACA ATGTGCTAGC 
GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA CCTGATGCTG CAAAGCAGGC AAACTCAGCG 
ATACCAAGTG TGCCAAAGGC TGGCAAAAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751270.1
CDS	205..3117
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895410.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895410.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT 
CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA 
GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG 
CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT 
CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG 
GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA 
CTAGATGATT CTGCTGGAGG AGCACTATTA ACTGATGCAC CCCCACCACC GCCAGAGGAA 
AAGCCTCTGA CCGATGATCA GCTGATTGAT AAGTTTTCTA TGGACTTTGA TTGTTCTGAA 
TCTCCTGCAA CACAACAGGC TCATCCTGTC GAACCTAAAG TTTCCTCGCA AGAGAAAAAG 
GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT GCTACTGTTA AAGCTGCACC CACTCCTTCT 
AAAGCACCAG CACCTCCAGC TGATCCACTC GATAGCCTGT CTGGTACCTT AGGTGTTCGA 
AAGGAAGAAC CGAGTGACAA GAAGCCTCCA GTGGATAAAG TAAAGGAGGG AACAGGCAAA 
GAGAAGAAAG AAAAGCTTGG AGAAGATGAA AAAACAATAC ATCCTGACCG CCGGCTAAAG 
GAAGTGAAGG ATAAAGACGG AAAGCCCTTG CTGCCAAAAC TTGAACAAAG CCCTAAGCCA 
ATGAGTGAGG CCGAGCTGCT GGATGCTTTG ACTGAAGGCT TTGTGACTTC CCCTGCCACT 
CCTGATGCCC CACTGCAGGG CTCTTTGAAA AAAAAAGGTT CTGGATCAGA AGATGTTATA 
TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT GGAGCTCCAC AGGACGCTGC TTTAGATATA 
CAGATTCCCG ACGATGCACT AGATTTACTT TCTGGCTCCC TGGGACAAAG AGAGGTGGAT 
CCTAATGAAA ACAAACCGGT GGTGGATGTA GTTAAGGAAA AAGCTAAAGC TGAGCATATA 
GACAAACTGG GGGACAGAGA TGATACAATC CCACCGGAAT ACAGAAAGCT GCTGGATGGT 
AAAGATGATA AGGGACAACC TGCAAAACCA CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG 
CCTTTAAGTG ACGATGCAGC GATCGATGCT CTTTCAAGCG GATTTGCATC ATGTGACACA 
AAATCTACTG AACAACCAAA GCAGCCTTCA ACGGACAAGA CTGAGAAACA ATGTGCTAGC 
GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA CCTGATGCTG CAAAGCAGGC AAACTCAGCG 
ATACCAAGTG TGCCAAAGGC TGGCAAAAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895413.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751273.1 Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OXa47527
Clone ID Related Accession (Same CDS sequence)	XM_031895413.1
Accession Version	XM_031895413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG 
CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA 
GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA 
AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC 
AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG 
ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT 
GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA 
AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT 
GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA 
CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA 
GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA 
AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA 
GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA 
AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA 
GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG 
AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG 
GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA 
GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA 
GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT 
CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT 
GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT 
GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT 
GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA 
GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG 
AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA 
TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT 
GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT 
GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC 
AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGATT CTGCTGGAGG AGCACTATTA 
ACTGATGCAC CCCCACCACC GCCAGAGGAA AAGCCTCTGA CCGATGATCA GCTGATTGAT 
AAGTTTTCTA TGGACTTTGA TTGTTCTGAA TCTCCTGCAA CACAACAGGC TCATCCTGTC 
GAACCTAAAG TTTCCTCGCA AGAGAAAAAG GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT 
GCTACTGTTA AAGCTGCACC CACTCCTTCT AAAGCACCAG CACCTCCAGC TGATCCACTC 
GATAGCCTGT CTGGTACCTT AGGTGTTCGA AAGGAAGAAC CGAGTGACAA GAAGCCTCCA 
GTGGATAAAG TAAAGGAGGG AACAGGCAAA GAGAAGAAAG AAAAGCTTGG AGAAGATGAA 
AAAACAATAC ATCCTGACCG CCGGCTAAAG GAAGTGAAGG ATAAAGACGG AAAGCCCTTG 
CTGCCAAAAC TTGAACAAAG CCCTAAGCCA ATGAGTGAGG CCGAGCTGCT GGATGCTTTG 
ACTGAAGGCT TTGTGACTTC CCCTGCCACT CCTGATGCCC CACTGCAGGG CTCTTTGAAA 
AAAAAAGGTT CTGGATCAGA AGATGTTATA TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT 
GGAGCTCCAC AGGACGCTGC TTTAGATATA CAGATTCCCG ACGATGCACT AGATTTACTT 
TCTGGCTCCC TGGGACAAAG AGAGGTGGAT CCTAATGAAA ACAAACCGGT GGTGGATGTA 
GTTAAGGAAA AAGCTAAAGC TGAGCATATA GACAAACTGG GGGACAGAGA TGATACAATC 
CCACCGGAAT ACAGAAAGCT GCTGGATGGT AAAGATGATA AGGGACAACC TGCAAAACCA 
CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG CCTTTAAGTG ACGATGCAGC GATCGATGCT 
CTTTCAAGCG GATTTGCATC ATGTGACACA AAATCTACTG AACAACCAAA GCAGCCTTCA 
ACGGACAAGA CTGAGAAACA ATGTGCTAGC GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA 
CCTGATGCTG CAAAGCAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751273.1
CDS	145..3027
Translation	MDTTQNNKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895413.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895413.1

ORF Insert Sequence:

ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG 
CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA 
GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA 
AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC 
AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG 
ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT 
GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA 
AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT 
GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA 
CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA 
GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA 
AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA 
GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA 
AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA 
GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG 
AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG 
GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA 
GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA 
GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT 
CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT 
GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT 
GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT 
GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA 
GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG 
AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA 
TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT 
GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT 
GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC 
AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGATT CTGCTGGAGG AGCACTATTA 
ACTGATGCAC CCCCACCACC GCCAGAGGAA AAGCCTCTGA CCGATGATCA GCTGATTGAT 
AAGTTTTCTA TGGACTTTGA TTGTTCTGAA TCTCCTGCAA CACAACAGGC TCATCCTGTC 
GAACCTAAAG TTTCCTCGCA AGAGAAAAAG GCAGAAAAAG AAGTAGTTGC TGCCTCCTCT 
GCTACTGTTA AAGCTGCACC CACTCCTTCT AAAGCACCAG CACCTCCAGC TGATCCACTC 
GATAGCCTGT CTGGTACCTT AGGTGTTCGA AAGGAAGAAC CGAGTGACAA GAAGCCTCCA 
GTGGATAAAG TAAAGGAGGG AACAGGCAAA GAGAAGAAAG AAAAGCTTGG AGAAGATGAA 
AAAACAATAC ATCCTGACCG CCGGCTAAAG GAAGTGAAGG ATAAAGACGG AAAGCCCTTG 
CTGCCAAAAC TTGAACAAAG CCCTAAGCCA ATGAGTGAGG CCGAGCTGCT GGATGCTTTG 
ACTGAAGGCT TTGTGACTTC CCCTGCCACT CCTGATGCCC CACTGCAGGG CTCTTTGAAA 
AAAAAAGGTT CTGGATCAGA AGATGTTATA TCTTGTTCTA AAGCTTCCGC CGTCAAGTCT 
GGAGCTCCAC AGGACGCTGC TTTAGATATA CAGATTCCCG ACGATGCACT AGATTTACTT 
TCTGGCTCCC TGGGACAAAG AGAGGTGGAT CCTAATGAAA ACAAACCGGT GGTGGATGTA 
GTTAAGGAAA AAGCTAAAGC TGAGCATATA GACAAACTGG GGGACAGAGA TGATACAATC 
CCACCGGAAT ACAGAAAGCT GCTGGATGGT AAAGATGATA AGGGACAACC TGCAAAACCA 
CCAGCGAAAG CGGAGGAGAA ACCAAAGAAG CCTTTAAGTG ACGATGCAGC GATCGATGCT 
CTTTCAAGCG GATTTGCATC ATGTGACACA AAATCTACTG AACAACCAAA GCAGCCTTCA 
ACGGACAAGA CTGAGAAACA ATGTGCTAGC GCTCCAGTGT CGCAGCCTTC AGGAAAGGCA 
CCTGATGCTG CAAAGCAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895421.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751281.1 Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OXa47528
Clone ID Related Accession (Same CDS sequence)	XM_031895421.1
Accession Version	XM_031895421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG 
CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA 
GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG 
AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG 
ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT 
CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT 
TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT 
GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT 
GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA 
GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG 
GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT 
CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA 
AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG 
TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT 
GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC 
TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT 
GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC 
GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT 
CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC 
TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA 
GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT 
CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG 
GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT 
GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA 
GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC 
AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT 
GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC 
GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG 
CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG 
GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC 
GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA 
CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG 
GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA 
GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA 
CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA 
GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA 
AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT 
TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751281.1
CDS	205..3039
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895421.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895421.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG 
CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA 
GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG 
AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG 
ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT 
CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT 
TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT 
GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT 
GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA 
GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG 
GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT 
CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA 
AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG 
TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT 
GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC 
TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT 
GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC 
GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT 
CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC 
TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGA TTCTGCTGGA 
GGAGCACTAT TAACTGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT 
CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG 
GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT 
GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA 
GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC 
AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT 
GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC 
GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG 
CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG 
GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC 
GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA 
CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG 
GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA 
GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA 
CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA 
GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA 
AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT 
TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895424.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751284.1 Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OXa47529
Clone ID Related Accession (Same CDS sequence)	XM_031895424.1
Accession Version	XM_031895424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA 
GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA 
GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA 
GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG 
GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA 
GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT 
GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG 
CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC 
ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC 
CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA 
GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT 
TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA 
AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT 
CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC 
ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA 
GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA 
ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA 
TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT 
ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT 
ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC 
TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG 
AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT 
ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT 
GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG 
GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGATTCT 
GCTGGAGGAG CACTATTAAC TGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC 
GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA 
CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA 
GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA 
CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG 
AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA 
AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT 
AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751284.1
CDS	229..3009
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895424.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895424.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA 
GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA 
GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA 
GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG 
GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA 
GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT 
GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG 
CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC 
ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC 
CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA 
GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT 
TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA 
AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT 
CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC 
ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA 
GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA 
ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA 
TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT 
ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT 
ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC 
TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG 
AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT 
ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT 
GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG 
GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGATTCT 
GCTGGAGGAG CACTATTAAC TGATGCACCC CCACCACCGC CAGAGGAAAA GCCTCTGACC 
GATGATCAGC TGATTGATAA GTTTTCTATG GACTTTGATT GTTCTGAATC TCCTGCAACA 
CAACAGGCTC ATCCTGTCGA ACCTAAAGTT TCCTCGCAAG AGAAAAAGGC AGAAAAAGAA 
GTAGTTGCTG CCTCCTCTGC TACTGTTAAA GCTGCACCCA CTCCTTCTAA AGCACCAGCA 
CCTCCAGCTG ATCCACTCGA TAGCCTGTCT GGTACCTTAG GTGTTCGAAA GGAAGAACCG 
AGTGACAAGA AGCCTCCAGT GGATAAAGTA AAGGAGGGAA CAGGCAAAGA GAAGAAAGAA 
AAGCTTGGAG AAGATGAAAA AACAATACAT CCTGACCGCC GGCTAAAGGA AGTGAAGGAT 
AAAGACGGAA AGCCCTTGCT GCCAAAACTT GAACAAAGCC CTAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956017.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811471.1 Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.

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CloneID	OXa31725
Clone ID Related Accession (Same CDS sequence)	XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
Accession Version	XM_012956017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811471.1
CDS	205..3213
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956017.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956017.2

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895362.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751222.1 Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47517
Clone ID Related Accession (Same CDS sequence)	XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
Accession Version	XM_031895362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751222.1
CDS	205..3222
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895362.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895362.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895367.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751227.1 Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa47517
Clone ID Related Accession (Same CDS sequence)	XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
Accession Version	XM_031895367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751227.1
CDS	209..3226
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895367.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895367.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895374.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751234.1 Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa47517
Clone ID Related Accession (Same CDS sequence)	XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
Accession Version	XM_031895374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751234.1
CDS	212..3229
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895374.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895374.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895373.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751233.1 Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa47517
Clone ID Related Accession (Same CDS sequence)	XM_031895362.1 , XM_031895367.1 , XM_031895374.1 , XM_031895373.1
Accession Version	XM_031895373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751233.1
CDS	134..3151
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895373.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895373.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895377.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751237.1 Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa47518
Clone ID Related Accession (Same CDS sequence)	XM_031895377.1
Accession Version	XM_031895377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751237.1
CDS	205..3219
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_031895377.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895377.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_031895380.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751240.1 Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa47519
Clone ID Related Accession (Same CDS sequence)	XM_031895380.1
Accession Version	XM_031895380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCAGTGCT TTTTAAACCT 
TCCTTGCACC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751240.1
CDS	205..3219
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDDSAGGALLTDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAVLFKPSLHH

Target ORF information:

RefSeq Version	XM_031895380.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895380.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGATTCTGCT 
GGAGGAGCAC TATTAACTGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCAGTGCT TTTTAAACCT 
TCCTTGCACC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( NM_001126837.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120309.1 Xenopus tropicalis calpastatin (cast), mRNA.

Price & Availability↑

CloneID	OXa21874
Clone ID Related Accession (Same CDS sequence)	NM_001126837.1
Accession Version	NM_001126837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC160635.1. ##Evidence-Data-START## Transcript exon combination :: BC160635.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG 
CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCCAGCGG CTGCAACCAA ACCAACAGCA 
GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA 
AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC 
AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGGTG 
ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT 
GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA 
AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT 
GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA 
CAATTTACTG GACCAGAAAT TCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA 
GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA 
AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA 
GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA 
AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA 
GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG 
AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG 
GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA 
GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC 
GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGAGTCTCC AGTATTTCAT 
GGTCCTGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT 
ATTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG 
TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CACAGCTTGT AGATGAGTTT 
TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC 
AAAGAAACTC CTCAAGGTAA CAAAACGAAA CCTGAAATTG CAGCATCATC TGCATCCACT 
GTAAAAGCAG CAGCGCCCTC CGGTGGTATG GACAGTGCAT TAGATGAATT ATTGGGAACA 
CTGGAAGGTC CTGATCCAAC AGCCCCTGAA TCACCTGTGT ACACTGGGCC AGAGGTGACG 
GAAACAGAGA CAGCGGTATA CAGAGAGGGA CTTGGGATAA GAGACAGCAG CATCCCTCCA 
GCATACAGGC ACTTACTAGA TGGGAAAGTA GATGGAAAAG ATGCACCCCC ACCACCGCCA 
GAGGAAAAGC CTCTGACCGA TGATCAGCTG ATTGATGAGT TTTCTATGGA CTTTGATTGT 
TCTGAATCTC CTGCAACACA ACAGGCTCAT CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG 
AAAAAGGCAG AAAAAGAAGT AGTCGCTGCC TCCTCTGCTA CTGTTAAAGC TGCACCCACT 
CCTTCTAAAG CACCAGCACC TCCAGCTGAT CCACTCGATA GCCTGTCTGG TACCTTAGGT 
TTTCGAAAGG AAGAACCGAG TGACAAGAAG CCTCCAGTGG ATAAAGTAAA GGAGGGAACA 
GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA GATGAAAAAA CAATACATCC TGACCGCCGG 
CTAAAGGAAG TGAAGGATAA AGATGGAAAG CCCTTGCTGC CAAAACTTGA ACAAAGCCCT 
AAGCCAATGA GTGAGGCCGA GCTGCTGGAT GCTTTGACTG AAGGCTTTGT GACTTCCCCT 
GCCACTCCTG ATGCCCCACT GCAGGGCTCT CTGAAAAAAA AAGGTTCTGG ATCAGAAGAT 
GTTATATCTT GTTCTAAAGC TTCCGCCGTC AAGTCTGGAG CTCCACAGGA CGCTGCTTTA 
GATATACAGA TTCCCGACGA TGCACTAGAT TTACTTTCTG GCTCCCTGGG ACAAAGAGAG 
GTGGATCCTA ATGAAAACAA ACCGGTGGTG GATGTAGTTA AGGAAAAAGC TAAAGCTGAG 
CATATAGACA AACTGGGGGA CAGAGATGAT ACAATCCCAC CGGAATACAG AAAGCTGCTG 
GATGGTAAAG ATGATAAGGG ACAACCTGCA AAACCACCAG CGAAAGCGGA GGAGAAACCA 
AAGAAGCCTT TAAGTGACGA TGCAGCGATC GATGCTCTTT CAAGCGGATT TGCATCATGT 
GACACAAAAT CTACTGAACA ACCAAAGCAG CCTTCAACGG ACAAGACTGA GAAACAATGT 
GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA AAGGCACCTG ATGCTGCAAA GGCAAACTCA 
GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120309.1
CDS	32..2827
Translation	MDTTQNNKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAAPAAATKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKVTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPESPVFHGPEVTENITATYLEETGKRDITIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTQLVDEFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDEFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGFRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	NM_001126837.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126837.1

ORF Insert Sequence:

ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG 
CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCCAGCGG CTGCAACCAA ACCAACAGCA 
GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA 
AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC 
AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGGTG 
ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT 
GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA 
AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT 
GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA 
CAATTTACTG GACCAGAAAT TCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA 
GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA 
AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA 
GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA 
AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA 
GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG 
AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG 
GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA 
GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC 
GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGAGTCTCC AGTATTTCAT 
GGTCCTGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT 
ATTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG 
TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CACAGCTTGT AGATGAGTTT 
TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC 
AAAGAAACTC CTCAAGGTAA CAAAACGAAA CCTGAAATTG CAGCATCATC TGCATCCACT 
GTAAAAGCAG CAGCGCCCTC CGGTGGTATG GACAGTGCAT TAGATGAATT ATTGGGAACA 
CTGGAAGGTC CTGATCCAAC AGCCCCTGAA TCACCTGTGT ACACTGGGCC AGAGGTGACG 
GAAACAGAGA CAGCGGTATA CAGAGAGGGA CTTGGGATAA GAGACAGCAG CATCCCTCCA 
GCATACAGGC ACTTACTAGA TGGGAAAGTA GATGGAAAAG ATGCACCCCC ACCACCGCCA 
GAGGAAAAGC CTCTGACCGA TGATCAGCTG ATTGATGAGT TTTCTATGGA CTTTGATTGT 
TCTGAATCTC CTGCAACACA ACAGGCTCAT CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG 
AAAAAGGCAG AAAAAGAAGT AGTCGCTGCC TCCTCTGCTA CTGTTAAAGC TGCACCCACT 
CCTTCTAAAG CACCAGCACC TCCAGCTGAT CCACTCGATA GCCTGTCTGG TACCTTAGGT 
TTTCGAAAGG AAGAACCGAG TGACAAGAAG CCTCCAGTGG ATAAAGTAAA GGAGGGAACA 
GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA GATGAAAAAA CAATACATCC TGACCGCCGG 
CTAAAGGAAG TGAAGGATAA AGATGGAAAG CCCTTGCTGC CAAAACTTGA ACAAAGCCCT 
AAGCCAATGA GTGAGGCCGA GCTGCTGGAT GCTTTGACTG AAGGCTTTGT GACTTCCCCT 
GCCACTCCTG ATGCCCCACT GCAGGGCTCT CTGAAAAAAA AAGGTTCTGG ATCAGAAGAT 
GTTATATCTT GTTCTAAAGC TTCCGCCGTC AAGTCTGGAG CTCCACAGGA CGCTGCTTTA 
GATATACAGA TTCCCGACGA TGCACTAGAT TTACTTTCTG GCTCCCTGGG ACAAAGAGAG 
GTGGATCCTA ATGAAAACAA ACCGGTGGTG GATGTAGTTA AGGAAAAAGC TAAAGCTGAG 
CATATAGACA AACTGGGGGA CAGAGATGAT ACAATCCCAC CGGAATACAG AAAGCTGCTG 
GATGGTAAAG ATGATAAGGG ACAACCTGCA AAACCACCAG CGAAAGCGGA GGAGAAACCA 
AAGAAGCCTT TAAGTGACGA TGCAGCGATC GATGCTCTTT CAAGCGGATT TGCATCATGT 
GACACAAAAT CTACTGAACA ACCAAAGCAG CCTTCAACGG ACAAGACTGA GAAACAATGT 
GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA AAGGCACCTG ATGCTGCAAA GGCAAACTCA 
GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956026.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811480.1 Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa31725
Clone ID Related Accession (Same CDS sequence)	XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
Accession Version	XM_012956026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811480.1
CDS	212..3220
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956026.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956026.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956023.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811477.1 Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa31725
Clone ID Related Accession (Same CDS sequence)	XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
Accession Version	XM_012956023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811477.1
CDS	103..3111
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956023.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956023.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956017.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811471.1 Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31725
Clone ID Related Accession (Same CDS sequence)	XM_012956026.1 , XM_012956023.1 , XM_012956017.1 , XM_012956017.2
Accession Version	XM_012956017.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811471.1
CDS	206..3214
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956017.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956017.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCGATAC CAAGTGTGCC AAAGGCTGGC 
AAAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956032.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811486.1 Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa31726
Clone ID Related Accession (Same CDS sequence)	XM_012956032.1
Accession Version	XM_012956032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811486.1
CDS	206..3211
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956032.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956032.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956040.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811494.1 Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.

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CloneID	OXa31727
Clone ID Related Accession (Same CDS sequence)	XM_012956040.1
Accession Version	XM_012956040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCAGTGC TTTTTAAACC TTCCTTGCAC 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811494.1
CDS	206..3211
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAVLFKPSLHH

Target ORF information:

RefSeq Version	XM_012956040.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956040.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGGATAA AGACGGAAAG 
CCCTTGCTGC CAAAACTTGA ACAAAGCCCT AAGCCAATGA GTGAGGCCGA GCTGCTGGAT 
GCTTTGACTG AAGGCTTTGT GACTTCCCCT GCCACTCCTG ATGCCCCACT GCAGGGCTCT 
TTGAAAAAAA AAGGTTCTGG ATCAGAAGAT GTTATATCTT GTTCTAAAGC TTCCGCCGTC 
AAGTCTGGAG CTCCACAGGA CGCTGCTTTA GATATACAGA TTCCCGACGA TGCACTAGAT 
TTACTTTCTG GCTCCCTGGG ACAAAGAGAG GTGGATCCTA ATGAAAACAA ACCGGTGGTG 
GATGTAGTTA AGGAAAAAGC TAAAGCTGAG CATATAGACA AACTGGGGGA CAGAGATGAT 
ACAATCCCAC CGGAATACAG AAAGCTGCTG GATGGTAAAG ATGATAAGGG ACAACCTGCA 
AAACCACCAG CGAAAGCGGA GGAGAAACCA AAGAAGCCTT TAAGTGACGA TGCAGCGATC 
GATGCTCTTT CAAGCGGATT TGCATCATGT GACACAAAAT CTACTGAACA ACCAAAGCAG 
CCTTCAACGG ACAAGACTGA GAAACAATGT GCTAGCGCTC CAGTGTCGCA GCCTTCAGGA 
AAGGCACCTG ATGCTGCAAA GCAGGCAAAC TCAGCAGTGC TTTTTAAACC TTCCTTGCAC 
CACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956046.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811500.1 Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa31728
Clone ID Related Accession (Same CDS sequence)	XM_012956046.2
Accession Version	XM_012956046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT 
GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT 
AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA 
ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA 
GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC 
AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT 
GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT 
GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA 
GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT 
ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC 
GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA 
CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT 
TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC 
CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG 
TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG 
AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA 
AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA 
AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA 
GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA 
GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC 
GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG 
GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT 
AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG 
TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT 
TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC 
AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA 
TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC 
ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA 
ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG 
ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT 
CCAGCATACA GGCACTTACT AGATGGGAAA GTAGATGGAA AAGATGCACC CCCACCACCG 
CCAGAGGAAA AGCCTCTGAC CGATGATCAG CTGATTGATA AGTTTTCTAT GGACTTTGAT 
TGTTCTGAAT CTCCTGCAAC ACAACAGGCT CATCCTGTCG AACCTAAAGT TTCCTCGCAA 
GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT GCCTCCTCTG CTACTGTTAA AGCTGCACCC 
ACTCCTTCTA AAGCACCAGC ACCTCCAGCT GATCCACTCG ATAGCCTGTC TGGTACCTTA 
GGTGTTCGAA AGGAAGAACC GAGTGACAAG AAGCCTCCAG TGGATAAAGT AAAGGAGGGA 
ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA GAAGATGAAA AAACAATACA TCCTGACCGC 
CGGCTAAAGG AAGTGAAGGA TAAAGACGGA AAGCCCTTGC TGCCAAAACT TGAACAAAGC 
CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG GATGCTTTGA CTGAAGGCTT TGTGACTTCC 
CCTGCCACTC CTGATGCCCC ACTGCAGGGC TCTTTGAAAA AAAAAGGTTC TGGATCAGAA 
GATGTTATAT CTTGTTCTAA AGCTTCCGCC GTCAAGTCTG GAGCTCCACA GGACGCTGCT 
TTAGATATAC AGATTCCCGA CGATGCACTA GATTTACTTT CTGGCTCCCT GGGACAAAGA 
GAGGTGGATC CTAATGAAAA CAAACCGGTG GTGGATGTAG TTAAGGAAAA AGCTAAAGCT 
GAGCATATAG ACAAACTGGG GGACAGAGAT GATACAATCC CACCGGAATA CAGAAAGCTG 
CTGGATGGTA AAGATGATAA GGGACAACCT GCAAAACCAC CAGCGAAAGC GGAGGAGAAA 
CCAAAGAAGC CTTTAAGTGA CGATGCAGCG ATCGATGCTC TTTCAAGCGG ATTTGCATCA 
TGTGACACAA AATCTACTGA ACAACCAAAG CAGCCTTCAA CGGACAAGAC TGAGAAACAA 
TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA GGAAAGGCAC CTGATGCTGC AAAGCAGGCA 
AACTCAGCGA TACCAAGTGT GCCAAAGGCT GGCAAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811500.1
CDS	210..3191
Translation	MAFASWWYATQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956046.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956046.2

ORF Insert Sequence:

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAA GAAAACTGGT GCCAGTGGCC CTTCAGGGGC CCAAGCAAGT 
GGGACCAGCA AGACTCCTAG TGCTTCAGGA AAAACTATGA ACCCGGCGGA TACACAGGCT 
AAACAAACAC CCAAGACTGA ACCAGCCAAA TCTACAAAGC CAACAAAGCC AAAAGAAGCA 
ACAACAGCAT CTTCAGCAGC CCTGGCGGCT GCATCCAAAC CAACAGCAGC ACCTTCCTCA 
GCCCCAGTAA CAGCAACCAA ACCAACAAAA ACAAAGCCAA GTGCAGCAAA ATCGCCTCCC 
AAACAAGATA TAAAGCCTAT CCCTGGGAAG CAGCAAAAAA CAGACAGCAA GAGTAAAGAT 
GAAAAGAAAA GTGGTGATAA AGTTTCTCCA GCTGTGCTAC CTAAGATGAC ACCTAGTTCT 
GCTGGTAAAG TATCTGCAGT TGCCACTGGA GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA 
GTCACTGCTG TGCAGTCAAC AAAGCCGAAA GATGAGAGTA AAGAGGAAAA GAAAGCCATT 
ACTGGGGATA AGGTTTCTTC TGGGCCACCG GCCAAGACAG ACGCAGATGC AGCCTTGGAC 
GAACTACTTG GAACGTTAGA GGGCCCAGCA GATATTCCAG AGTCTCCACA ATTTACTGGA 
CCAGAAATCC AGGACACAGT TGTTACATCT GAGTATTTGG AGGAGCTAGG AAAACGGGAT 
TTCACCATCC CACCTGAATA CAGGCACCTG CTGGATGGTA AAGGTGAAAA GATGGCACCC 
CCTACCCCAC CCGAGGAACC CGAAGAGCAC ATGGATGATG ATGAATTAGT TGCAGCCCTG 
TCTTCTGGTT TTGAGAGCAG TCAGGCATCC CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG 
AAAATGGAAG TGAAACCAAA ATCAGCAGCA GCAGTGGCCA CACAGCCAGA TGTGCAGAAA 
AAAGAAGTAA AAGTCGCAGC AGCAGAAACT TCCAAACCAA GCACTCAGAA AAAAGAGGAA 
AAAGCAAAAA TAGCAGAAGT GTCTAAACCA AGCACTCAGA AAAAAGAGGA AAAACTGGCA 
GCAGCAGCAG ACACATCCAA ACCAAGCACT CAGAAAAAAG AGGTAAAAGC CACAGCAAAA 
GCCACAGCTG CCGAAGCTTC CAAACAAAGC ACTGAGTCCA TACCATCAGA AGCAATAGAC 
GAGTTGTTGG GAACACTTGA AGGACCCCCA GAAAATGTTC CAGGGTCTCC AGTATTTCAG 
GGTCCAGAAG TTACTGAGAA CATTACAGCA ACATACTTAG AAGAAACTGG AAAAAGAGAT 
AGTACGATCC CTCCAGAATA CAGAAAATTA TTAGATGGAA AGGATCATGG GAAAGATGTG 
TGGCCTTCAG CACCTGAACC AGAACCGTCT ATGACCGACA CAGAGCTTGT AGATGTGTTT 
TCCAAAGACT TTGAGCTTTC ATGTTCACCT ACTGTTCAGA CCACGCCAGC CATTAAACCC 
AAAAGGAACA CTGAAGATCT CCACGAGTGC TATTGCTCAA AGTCAGTGAG CCAACTTTCA 
TCATCTGAAA CTCCTCAAGG TAACAAAACG AAACCTGAAA TTGCGGCATC ATCTGCATCC 
ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT ATGGACAGTG CATTAGATGA ATTATTGGGA 
ACACTGGAAG GTCCTGATCC AACAGCCCCT GAATCACCTG TGTACACTGG GCCAGAGGTG 
ACGGAAACAG AGACAGCGGT ATACAGAGAG GGACTTGGGA TAAGAGACAG CAGCATCCCT 
CCAGCATACA GGCACTTACT AGATGGGAAA GTAGATGGAA AAGATGCACC CCCACCACCG 
CCAGAGGAAA AGCCTCTGAC CGATGATCAG CTGATTGATA AGTTTTCTAT GGACTTTGAT 
TGTTCTGAAT CTCCTGCAAC ACAACAGGCT CATCCTGTCG AACCTAAAGT TTCCTCGCAA 
GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT GCCTCCTCTG CTACTGTTAA AGCTGCACCC 
ACTCCTTCTA AAGCACCAGC ACCTCCAGCT GATCCACTCG ATAGCCTGTC TGGTACCTTA 
GGTGTTCGAA AGGAAGAACC GAGTGACAAG AAGCCTCCAG TGGATAAAGT AAAGGAGGGA 
ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA GAAGATGAAA AAACAATACA TCCTGACCGC 
CGGCTAAAGG AAGTGAAGGA TAAAGACGGA AAGCCCTTGC TGCCAAAACT TGAACAAAGC 
CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG GATGCTTTGA CTGAAGGCTT TGTGACTTCC 
CCTGCCACTC CTGATGCCCC ACTGCAGGGC TCTTTGAAAA AAAAAGGTTC TGGATCAGAA 
GATGTTATAT CTTGTTCTAA AGCTTCCGCC GTCAAGTCTG GAGCTCCACA GGACGCTGCT 
TTAGATATAC AGATTCCCGA CGATGCACTA GATTTACTTT CTGGCTCCCT GGGACAAAGA 
GAGGTGGATC CTAATGAAAA CAAACCGGTG GTGGATGTAG TTAAGGAAAA AGCTAAAGCT 
GAGCATATAG ACAAACTGGG GGACAGAGAT GATACAATCC CACCGGAATA CAGAAAGCTG 
CTGGATGGTA AAGATGATAA GGGACAACCT GCAAAACCAC CAGCGAAAGC GGAGGAGAAA 
CCAAAGAAGC CTTTAAGTGA CGATGCAGCG ATCGATGCTC TTTCAAGCGG ATTTGCATCA 
TGTGACACAA AATCTACTGA ACAACCAAAG CAGCCTTCAA CGGACAAGAC TGAGAAACAA 
TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA GGAAAGGCAC CTGATGCTGC AAAGCAGGCA 
AACTCAGCGA TACCAAGTGT GCCAAAGGCT GGCAAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956054.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811508.1 Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa31729
Clone ID Related Accession (Same CDS sequence)	XM_012956054.1
Accession Version	XM_012956054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA 
AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA 
CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA 
CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT 
ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA 
AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA 
CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT 
TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC 
GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC 
AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC 
GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA 
GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC 
AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT 
GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811508.1
CDS	206..3163
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956054.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956054.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA 
AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA 
CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA 
CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT 
ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA 
AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA 
CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT 
TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC 
GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC 
AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC 
GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA 
GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC 
AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT 
GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956066.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811520.1 Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OXa31731
Clone ID Related Accession (Same CDS sequence)	XM_012956066.1
Accession Version	XM_012956066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG 
GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA 
AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG 
AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT 
CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT 
GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG 
AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA 
CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA 
GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA 
TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC 
CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG 
CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA 
TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA 
GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA 
ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC 
ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA 
AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC 
CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA 
GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA 
TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG 
TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA 
CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG 
TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA 
ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT 
GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC 
CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA 
GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGGG 
AAAGTAGATG GAAAAGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT 
CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG 
GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT 
GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA 
GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC 
AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT 
GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC 
GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG 
CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG 
GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC 
GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA 
CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG 
GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA 
GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA 
CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA 
GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA 
AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT 
TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811520.1
CDS	206..3160
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956066.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956066.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GCCAAAAGAA GCAACAACAG CATCTTCAGC AGCCCTGGCG 
GCTGCATCCA AACCAACAGC AGCACCTTCC TCAGCCCCAG TAACAGCAAC CAAACCAACA 
AAAACAAAGC CAAGTGCAGC AAAATCGCCT CCCAAACAAG ATATAAAGCC TATCCCTGGG 
AAGCAGCAAA AAACAGACAG CAAGAGTAAA GATGAAAAGA AAAGTGGTGA TAAAGTTTCT 
CCAGCTGTGC TACCTAAGAT GACACCTAGT TCTGCTGGTA AAGTATCTGC AGTTGCCACT 
GGAGCTCTAG CAGCTGCTGG TGCCGCCGCT GCAGTCACTG CTGTGCAGTC AACAAAGCCG 
AAAGATGAGA GTAAAGAGGA AAAGAAAGCC ATTACTGGGG ATAAGGTTTC TTCTGGGCCA 
CCGGCCAAGA CAGACGCAGA TGCAGCCTTG GACGAACTAC TTGGAACGTT AGAGGGCCCA 
GCAGATATTC CAGAGTCTCC ACAATTTACT GGACCAGAAA TCCAGGACAC AGTTGTTACA 
TCTGAGTATT TGGAGGAGCT AGGAAAACGG GATTTCACCA TCCCACCTGA ATACAGGCAC 
CTGCTGGATG GTAAAGGTGA AAAGATGGCA CCCCCTACCC CACCCGAGGA ACCCGAAGAG 
CACATGGATG ATGATGAATT AGTTGCAGCC CTGTCTTCTG GTTTTGAGAG CAGTCAGGCA 
TCCCCCGAAG TAAAGAAGCC AAAGCTGGAA GAGAAAATGG AAGTGAAACC AAAATCAGCA 
GCAGCAGTGG CCACACAGCC AGATGTGCAG AAAAAAGAAG TAAAAGTCGC AGCAGCAGAA 
ACTTCCAAAC CAAGCACTCA GAAAAAAGAG GAAAAAGCAA AAATAGCAGA AGTGTCTAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAACTG GCAGCAGCAG CAGACACATC CAAACCAAGC 
ACTCAGAAAA AAGAGGTAAA AGCCACAGCA AAAGCCACAG CTGCCGAAGC TTCCAAACAA 
AGCACTGAGT CCATACCATC AGAAGCAATA GACGAGTTGT TGGGAACACT TGAAGGACCC 
CCAGAAAATG TTCCAGGGTC TCCAGTATTT CAGGGTCCAG AAGTTACTGA GAACATTACA 
GCAACATACT TAGAAGAAAC TGGAAAAAGA GATAGTACGA TCCCTCCAGA ATACAGAAAA 
TTATTAGATG GAAAGGATCA TGGGAAAGAT GTGTGGCCTT CAGCACCTGA ACCAGAACCG 
TCTATGACCG ACACAGAGCT TGTAGATGTG TTTTCCAAAG ACTTTGAGCT TTCATGTTCA 
CCTACTGTTC AGACCACGCC AGCCATTAAA CCCAAAAGGA ACACTGAAGA TCTCCACGAG 
TGCTATTGCT CAAAGTCAGT GAGCCAACTT TCATCATCTG AAACTCCTCA AGGTAACAAA 
ACGAAACCTG AAATTGCGGC ATCATCTGCA TCCACTGTAA AAGCAGCAGC GCCCTCCGGT 
GGTATGGACA GTGCATTAGA TGAATTATTG GGAACACTGG AAGGTCCTGA TCCAACAGCC 
CCTGAATCAC CTGTGTACAC TGGGCCAGAG GTGACGGAAA CAGAGACAGC GGTATACAGA 
GAGGGACTTG GGATAAGAGA CAGCAGCATC CCTCCAGCAT ACAGGCACTT ACTAGATGGG 
AAAGTAGATG GAAAAGATGC ACCCCCACCA CCGCCAGAGG AAAAGCCTCT GACCGATGAT 
CAGCTGATTG ATAAGTTTTC TATGGACTTT GATTGTTCTG AATCTCCTGC AACACAACAG 
GCTCATCCTG TCGAACCTAA AGTTTCCTCG CAAGAGAAAA AGGCAGAAAA AGAAGTAGTT 
GCTGCCTCCT CTGCTACTGT TAAAGCTGCA CCCACTCCTT CTAAAGCACC AGCACCTCCA 
GCTGATCCAC TCGATAGCCT GTCTGGTACC TTAGGTGTTC GAAAGGAAGA ACCGAGTGAC 
AAGAAGCCTC CAGTGGATAA AGTAAAGGAG GGAACAGGCA AAGAGAAGAA AGAAAAGCTT 
GGAGAAGATG AAAAAACAAT ACATCCTGAC CGCCGGCTAA AGGAAGTGAA GGATAAAGAC 
GGAAAGCCCT TGCTGCCAAA ACTTGAACAA AGCCCTAAGC CAATGAGTGA GGCCGAGCTG 
CTGGATGCTT TGACTGAAGG CTTTGTGACT TCCCCTGCCA CTCCTGATGC CCCACTGCAG 
GGCTCTTTGA AAAAAAAAGG TTCTGGATCA GAAGATGTTA TATCTTGTTC TAAAGCTTCC 
GCCGTCAAGT CTGGAGCTCC ACAGGACGCT GCTTTAGATA TACAGATTCC CGACGATGCA 
CTAGATTTAC TTTCTGGCTC CCTGGGACAA AGAGAGGTGG ATCCTAATGA AAACAAACCG 
GTGGTGGATG TAGTTAAGGA AAAAGCTAAA GCTGAGCATA TAGACAAACT GGGGGACAGA 
GATGATACAA TCCCACCGGA ATACAGAAAG CTGCTGGATG GTAAAGATGA TAAGGGACAA 
CCTGCAAAAC CACCAGCGAA AGCGGAGGAG AAACCAAAGA AGCCTTTAAG TGACGATGCA 
GCGATCGATG CTCTTTCAAG CGGATTTGCA TCATGTGACA CAAAATCTAC TGAACAACCA 
AAGCAGCCTT CAACGGACAA GACTGAGAAA CAATGTGCTA GCGCTCCAGT GTCGCAGCCT 
TCAGGAAAGG CACCTGATGC TGCAAAGCAG GCAAACTCAG CGATACCAAG TGTGCCAAAG 
GCTGGCAAAA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956074.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811528.1 Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OXa31732
Clone ID Related Accession (Same CDS sequence)	XM_012956074.2 , XM_012956074.1
Accession Version	XM_012956074.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811528.1
CDS	206..3151
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956074.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956074.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956081.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811535.1 Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OXa31733
Clone ID Related Accession (Same CDS sequence)	XM_012956081.1 , XM_012956081.2
Accession Version	XM_012956081.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811535.1
CDS	206..3148
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956081.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956081.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGGC AAACTCAGCG ATACCAAGTG TGCCAAAGGC TGGCAAAAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956088.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811542.1 Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OXa31734
Clone ID Related Accession (Same CDS sequence)	XM_012956088.2
Accession Version	XM_012956088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA 
CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA 
GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT 
TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG 
CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT 
AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT 
AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC 
GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA 
GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC 
TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT 
ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA 
CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG 
GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA 
GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG 
GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG 
CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA 
GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA 
CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA 
GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA 
ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA 
TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA 
AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA 
GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT 
GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT 
AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA 
CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT 
GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA 
TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA 
GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC 
ATCCCTCCAG CATACAGGCA CTTACTAGAT GGGAAAGTAG ATGGAAAAGA TGCACCCCCA 
CCACCGCCAG AGGAAAAGCC TCTGACCGAT GATCAGCTGA TTGATAAGTT TTCTATGGAC 
TTTGATTGTT CTGAATCTCC TGCAACACAA CAGGCTCATC CTGTCGAACC TAAAGTTTCC 
TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT 
GCACCCACTC CTTCTAAAGC ACCAGCACCT CCAGCTGATC CACTCGATAG CCTGTCTGGT 
ACCTTAGGTG TTCGAAAGGA AGAACCGAGT GACAAGAAGC CTCCAGTGGA TAAAGTAAAG 
GAGGGAACAG GCAAAGAGAA GAAAGAAAAG CTTGGAGAAG ATGAAAAAAC AATACATCCT 
GACCGCCGGC TAAAGGAAGT GAAGGATAAA GACGGAAAGC CCTTGCTGCC AAAACTTGAA 
CAAAGCCCTA AGCCAATGAG TGAGGCCGAG CTGCTGGATG CTTTGACTGA AGGCTTTGTG 
ACTTCCCCTG CCACTCCTGA TGCCCCACTG CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA 
TCAGAAGATG TTATATCTTG TTCTAAAGCT TCCGCCGTCA AGTCTGGAGC TCCACAGGAC 
GCTGCTTTAG ATATACAGAT TCCCGACGAT GCACTAGATT TACTTTCTGG CTCCCTGGGA 
CAAAGAGAGG TGGATCCTAA TGAAAACAAA CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT 
AAAGCTGAGC ATATAGACAA ACTGGGGGAC AGAGATGATA CAATCCCACC GGAATACAGA 
AAGCTGCTGG ATGGTAAAGA TGATAAGGGA CAACCTGCAA AACCACCAGC GAAAGCGGAG 
GAGAAACCAA AGAAGCCTTT AAGTGACGAT GCAGCGATCG ATGCTCTTTC AAGCGGATTT 
GCATCATGTG ACACAAAATC TACTGAACAA CCAAAGCAGC CTTCAACGGA CAAGACTGAG 
AAACAATGTG CTAGCGCTCC AGTGTCGCAG CCTTCAGGAA AGGCACCTGA TGCTGCAAAG 
CAGGCAAACT CAGCGATACC AAGTGTGCCA AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811542.1
CDS	210..3137
Translation	MAFASWWYATQCDRKPADKKPSEGAETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956088.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956088.2

ORF Insert Sequence:

ATGGCCTTTG CAAGCTGGTG GTATGCTACG CAATGTGATA GGAAGCCTGC AGACAAGAAG 
CCATCAGAAG GAGCAGAGAC TCCTAGTGCT TCAGGAAAAA CTATGAACCC GGCGGATACA 
CAGGCTAAAC AAACACCCAA GACTGAACCA GCCAAATCTA CAAAGCCAAC AAAGCCAAAA 
GAAGCAACAA CAGCATCTTC AGCAGCCCTG GCGGCTGCAT CCAAACCAAC AGCAGCACCT 
TCCTCAGCCC CAGTAACAGC AACCAAACCA ACAAAAACAA AGCCAAGTGC AGCAAAATCG 
CCTCCCAAAC AAGATATAAA GCCTATCCCT GGGAAGCAGC AAAAAACAGA CAGCAAGAGT 
AAAGATGAAA AGAAAAGTGG TGATAAAGTT TCTCCAGCTG TGCTACCTAA GATGACACCT 
AGTTCTGCTG GTAAAGTATC TGCAGTTGCC ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC 
GCTGCAGTCA CTGCTGTGCA GTCAACAAAG CCGAAAGATG AGAGTAAAGA GGAAAAGAAA 
GCCATTACTG GGGATAAGGT TTCTTCTGGG CCACCGGCCA AGACAGACGC AGATGCAGCC 
TTGGACGAAC TACTTGGAAC GTTAGAGGGC CCAGCAGATA TTCCAGAGTC TCCACAATTT 
ACTGGACCAG AAATCCAGGA CACAGTTGTT ACATCTGAGT ATTTGGAGGA GCTAGGAAAA 
CGGGATTTCA CCATCCCACC TGAATACAGG CACCTGCTGG ATGGTAAAGG TGAAAAGATG 
GCACCCCCTA CCCCACCCGA GGAACCCGAA GAGCACATGG ATGATGATGA ATTAGTTGCA 
GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG GCATCCCCCG AAGTAAAGAA GCCAAAGCTG 
GAAGAGAAAA TGGAAGTGAA ACCAAAATCA GCAGCAGCAG TGGCCACACA GCCAGATGTG 
CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA GAAACTTCCA AACCAAGCAC TCAGAAAAAA 
GAGGAAAAAG CAAAAATAGC AGAAGTGTCT AAACCAAGCA CTCAGAAAAA AGAGGAAAAA 
CTGGCAGCAG CAGCAGACAC ATCCAAACCA AGCACTCAGA AAAAAGAGGT AAAAGCCACA 
GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA CAAAGCACTG AGTCCATACC ATCAGAAGCA 
ATAGACGAGT TGTTGGGAAC ACTTGAAGGA CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA 
TTTCAGGGTC CAGAAGTTAC TGAGAACATT ACAGCAACAT ACTTAGAAGA AACTGGAAAA 
AGAGATAGTA CGATCCCTCC AGAATACAGA AAATTATTAG ATGGAAAGGA TCATGGGAAA 
GATGTGTGGC CTTCAGCACC TGAACCAGAA CCGTCTATGA CCGACACAGA GCTTGTAGAT 
GTGTTTTCCA AAGACTTTGA GCTTTCATGT TCACCTACTG TTCAGACCAC GCCAGCCATT 
AAACCCAAAA GGAACACTGA AGATCTCCAC GAGTGCTATT GCTCAAAGTC AGTGAGCCAA 
CTTTCATCAT CTGAAACTCC TCAAGGTAAC AAAACGAAAC CTGAAATTGC GGCATCATCT 
GCATCCACTG TAAAAGCAGC AGCGCCCTCC GGTGGTATGG ACAGTGCATT AGATGAATTA 
TTGGGAACAC TGGAAGGTCC TGATCCAACA GCCCCTGAAT CACCTGTGTA CACTGGGCCA 
GAGGTGACGG AAACAGAGAC AGCGGTATAC AGAGAGGGAC TTGGGATAAG AGACAGCAGC 
ATCCCTCCAG CATACAGGCA CTTACTAGAT GGGAAAGTAG ATGGAAAAGA TGCACCCCCA 
CCACCGCCAG AGGAAAAGCC TCTGACCGAT GATCAGCTGA TTGATAAGTT TTCTATGGAC 
TTTGATTGTT CTGAATCTCC TGCAACACAA CAGGCTCATC CTGTCGAACC TAAAGTTTCC 
TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT 
GCACCCACTC CTTCTAAAGC ACCAGCACCT CCAGCTGATC CACTCGATAG CCTGTCTGGT 
ACCTTAGGTG TTCGAAAGGA AGAACCGAGT GACAAGAAGC CTCCAGTGGA TAAAGTAAAG 
GAGGGAACAG GCAAAGAGAA GAAAGAAAAG CTTGGAGAAG ATGAAAAAAC AATACATCCT 
GACCGCCGGC TAAAGGAAGT GAAGGATAAA GACGGAAAGC CCTTGCTGCC AAAACTTGAA 
CAAAGCCCTA AGCCAATGAG TGAGGCCGAG CTGCTGGATG CTTTGACTGA AGGCTTTGTG 
ACTTCCCCTG CCACTCCTGA TGCCCCACTG CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA 
TCAGAAGATG TTATATCTTG TTCTAAAGCT TCCGCCGTCA AGTCTGGAGC TCCACAGGAC 
GCTGCTTTAG ATATACAGAT TCCCGACGAT GCACTAGATT TACTTTCTGG CTCCCTGGGA 
CAAAGAGAGG TGGATCCTAA TGAAAACAAA CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT 
AAAGCTGAGC ATATAGACAA ACTGGGGGAC AGAGATGATA CAATCCCACC GGAATACAGA 
AAGCTGCTGG ATGGTAAAGA TGATAAGGGA CAACCTGCAA AACCACCAGC GAAAGCGGAG 
GAGAAACCAA AGAAGCCTTT AAGTGACGAT GCAGCGATCG ATGCTCTTTC AAGCGGATTT 
GCATCATGTG ACACAAAATC TACTGAACAA CCAAAGCAGC CTTCAACGGA CAAGACTGAG 
AAACAATGTG CTAGCGCTCC AGTGTCGCAG CCTTCAGGAA AGGCACCTGA TGCTGCAAAG 
CAGGCAAACT CAGCGATACC AAGTGTGCCA AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956093.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811547.1 Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OXa31735
Clone ID Related Accession (Same CDS sequence)	XM_012956093.1
Accession Version	XM_012956093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG 
ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA 
GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA 
ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG 
CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT 
GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT 
GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG 
TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT 
TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG 
TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC 
ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT 
GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG 
GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG 
AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA 
CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC 
GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA 
GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA 
TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA 
GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA 
CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT 
GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA 
GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT 
GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG 
CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA 
GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT 
CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA 
GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT 
GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA 
GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC 
TTACTAGATG GGAAAGTAGA TGGAAAAGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT 
CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT 
GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA 
AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA 
CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA 
GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG 
AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG 
AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT 
GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT 
GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT 
TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT 
CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT 
GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA 
CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT 
GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA 
AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT 
ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA 
GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA 
AGTGTGCCAA AGGCTGGCAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811547.1
CDS	206..3112
Translation	MSKPGKGRKHSGPGKGTKETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956093.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956093.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGGCTAAACA AACACCCAAG 
ACTGAACCAG CCAAATCTAC AAAGCCAACA AAGCCAAAAG AAGCAACAAC AGCATCTTCA 
GCAGCCCTGG CGGCTGCATC CAAACCAACA GCAGCACCTT CCTCAGCCCC AGTAACAGCA 
ACCAAACCAA CAAAAACAAA GCCAAGTGCA GCAAAATCGC CTCCCAAACA AGATATAAAG 
CCTATCCCTG GGAAGCAGCA AAAAACAGAC AGCAAGAGTA AAGATGAAAA GAAAAGTGGT 
GATAAAGTTT CTCCAGCTGT GCTACCTAAG ATGACACCTA GTTCTGCTGG TAAAGTATCT 
GCAGTTGCCA CTGGAGCTCT AGCAGCTGCT GGTGCCGCCG CTGCAGTCAC TGCTGTGCAG 
TCAACAAAGC CGAAAGATGA GAGTAAAGAG GAAAAGAAAG CCATTACTGG GGATAAGGTT 
TCTTCTGGGC CACCGGCCAA GACAGACGCA GATGCAGCCT TGGACGAACT ACTTGGAACG 
TTAGAGGGCC CAGCAGATAT TCCAGAGTCT CCACAATTTA CTGGACCAGA AATCCAGGAC 
ACAGTTGTTA CATCTGAGTA TTTGGAGGAG CTAGGAAAAC GGGATTTCAC CATCCCACCT 
GAATACAGGC ACCTGCTGGA TGGTAAAGGT GAAAAGATGG CACCCCCTAC CCCACCCGAG 
GAACCCGAAG AGCACATGGA TGATGATGAA TTAGTTGCAG CCCTGTCTTC TGGTTTTGAG 
AGCAGTCAGG CATCCCCCGA AGTAAAGAAG CCAAAGCTGG AAGAGAAAAT GGAAGTGAAA 
CCAAAATCAG CAGCAGCAGT GGCCACACAG CCAGATGTGC AGAAAAAAGA AGTAAAAGTC 
GCAGCAGCAG AAACTTCCAA ACCAAGCACT CAGAAAAAAG AGGAAAAAGC AAAAATAGCA 
GAAGTGTCTA AACCAAGCAC TCAGAAAAAA GAGGAAAAAC TGGCAGCAGC AGCAGACACA 
TCCAAACCAA GCACTCAGAA AAAAGAGGTA AAAGCCACAG CAAAAGCCAC AGCTGCCGAA 
GCTTCCAAAC AAAGCACTGA GTCCATACCA TCAGAAGCAA TAGACGAGTT GTTGGGAACA 
CTTGAAGGAC CCCCAGAAAA TGTTCCAGGG TCTCCAGTAT TTCAGGGTCC AGAAGTTACT 
GAGAACATTA CAGCAACATA CTTAGAAGAA ACTGGAAAAA GAGATAGTAC GATCCCTCCA 
GAATACAGAA AATTATTAGA TGGAAAGGAT CATGGGAAAG ATGTGTGGCC TTCAGCACCT 
GAACCAGAAC CGTCTATGAC CGACACAGAG CTTGTAGATG TGTTTTCCAA AGACTTTGAG 
CTTTCATGTT CACCTACTGT TCAGACCACG CCAGCCATTA AACCCAAAAG GAACACTGAA 
GATCTCCACG AGTGCTATTG CTCAAAGTCA GTGAGCCAAC TTTCATCATC TGAAACTCCT 
CAAGGTAACA AAACGAAACC TGAAATTGCG GCATCATCTG CATCCACTGT AAAAGCAGCA 
GCGCCCTCCG GTGGTATGGA CAGTGCATTA GATGAATTAT TGGGAACACT GGAAGGTCCT 
GATCCAACAG CCCCTGAATC ACCTGTGTAC ACTGGGCCAG AGGTGACGGA AACAGAGACA 
GCGGTATACA GAGAGGGACT TGGGATAAGA GACAGCAGCA TCCCTCCAGC ATACAGGCAC 
TTACTAGATG GGAAAGTAGA TGGAAAAGAT GCACCCCCAC CACCGCCAGA GGAAAAGCCT 
CTGACCGATG ATCAGCTGAT TGATAAGTTT TCTATGGACT TTGATTGTTC TGAATCTCCT 
GCAACACAAC AGGCTCATCC TGTCGAACCT AAAGTTTCCT CGCAAGAGAA AAAGGCAGAA 
AAAGAAGTAG TTGCTGCCTC CTCTGCTACT GTTAAAGCTG CACCCACTCC TTCTAAAGCA 
CCAGCACCTC CAGCTGATCC ACTCGATAGC CTGTCTGGTA CCTTAGGTGT TCGAAAGGAA 
GAACCGAGTG ACAAGAAGCC TCCAGTGGAT AAAGTAAAGG AGGGAACAGG CAAAGAGAAG 
AAAGAAAAGC TTGGAGAAGA TGAAAAAACA ATACATCCTG ACCGCCGGCT AAAGGAAGTG 
AAGGATAAAG ACGGAAAGCC CTTGCTGCCA AAACTTGAAC AAAGCCCTAA GCCAATGAGT 
GAGGCCGAGC TGCTGGATGC TTTGACTGAA GGCTTTGTGA CTTCCCCTGC CACTCCTGAT 
GCCCCACTGC AGGGCTCTTT GAAAAAAAAA GGTTCTGGAT CAGAAGATGT TATATCTTGT 
TCTAAAGCTT CCGCCGTCAA GTCTGGAGCT CCACAGGACG CTGCTTTAGA TATACAGATT 
CCCGACGATG CACTAGATTT ACTTTCTGGC TCCCTGGGAC AAAGAGAGGT GGATCCTAAT 
GAAAACAAAC CGGTGGTGGA TGTAGTTAAG GAAAAAGCTA AAGCTGAGCA TATAGACAAA 
CTGGGGGACA GAGATGATAC AATCCCACCG GAATACAGAA AGCTGCTGGA TGGTAAAGAT 
GATAAGGGAC AACCTGCAAA ACCACCAGCG AAAGCGGAGG AGAAACCAAA GAAGCCTTTA 
AGTGACGATG CAGCGATCGA TGCTCTTTCA AGCGGATTTG CATCATGTGA CACAAAATCT 
ACTGAACAAC CAAAGCAGCC TTCAACGGAC AAGACTGAGA AACAATGTGC TAGCGCTCCA 
GTGTCGCAGC CTTCAGGAAA GGCACCTGAT GCTGCAAAGC AGGCAAACTC AGCGATACCA 
AGTGTGCCAA AGGCTGGCAA AAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956106.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811560.1 Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OXa31737
Clone ID Related Accession (Same CDS sequence)	XM_012956106.2
Accession Version	XM_012956106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG 
CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA 
GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA 
AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC 
AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG 
ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT 
GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA 
AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT 
GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA 
CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA 
GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA 
AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA 
GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA 
AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA 
GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG 
AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG 
GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA 
GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA 
GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT 
CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT 
GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT 
GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT 
GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA 
GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG 
AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA 
TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT 
GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT 
GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC 
AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGGGA AAGTAGATGG AAAAGATGCA 
CCCCCACCAC CGCCAGAGGA AAAGCCTCTG ACCGATGATC AGCTGATTGA TAAGTTTTCT 
ATGGACTTTG ATTGTTCTGA ATCTCCTGCA ACACAACAGG CTCATCCTGT CGAACCTAAA 
GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA GAAGTAGTTG CTGCCTCCTC TGCTACTGTT 
AAAGCTGCAC CCACTCCTTC TAAAGCACCA GCACCTCCAG CTGATCCACT CGATAGCCTG 
TCTGGTACCT TAGGTGTTCG AAAGGAAGAA CCGAGTGACA AGAAGCCTCC AGTGGATAAA 
GTAAAGGAGG GAACAGGCAA AGAGAAGAAA GAAAAGCTTG GAGAAGATGA AAAAACAATA 
CATCCTGACC GCCGGCTAAA GGAAGTGAAG GATAAAGACG GAAAGCCCTT GCTGCCAAAA 
CTTGAACAAA GCCCTAAGCC AATGAGTGAG GCCGAGCTGC TGGATGCTTT GACTGAAGGC 
TTTGTGACTT CCCCTGCCAC TCCTGATGCC CCACTGCAGG GCTCTTTGAA AAAAAAAGGT 
TCTGGATCAG AAGATGTTAT ATCTTGTTCT AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA 
CAGGACGCTG CTTTAGATAT ACAGATTCCC GACGATGCAC TAGATTTACT TTCTGGCTCC 
CTGGGACAAA GAGAGGTGGA TCCTAATGAA AACAAACCGG TGGTGGATGT AGTTAAGGAA 
AAAGCTAAAG CTGAGCATAT AGACAAACTG GGGGACAGAG ATGATACAAT CCCACCGGAA 
TACAGAAAGC TGCTGGATGG TAAAGATGAT AAGGGACAAC CTGCAAAACC ACCAGCGAAA 
GCGGAGGAGA AACCAAAGAA GCCTTTAAGT GACGATGCAG CGATCGATGC TCTTTCAAGC 
GGATTTGCAT CATGTGACAC AAAATCTACT GAACAACCAA AGCAGCCTTC AACGGACAAG 
ACTGAGAAAC AATGTGCTAG CGCTCCAGTG TCGCAGCCTT CAGGAAAGGC ACCTGATGCT 
GCAAAGCAGG CAAACTCAGC GATACCAAGT GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811560.1
CDS	154..3027
Translation	MDTTQNNKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956106.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956106.2

ORF Insert Sequence:

ATGGACACCA CTCAAAATAA TAAGACTCCT AGTGCTTCAG GAAAAACTAT GAACCCGGCG 
GATACACAGG CTAAACAAAC ACCCAAGACT GAACCAGCCA AATCTACAAA GCCAACAAAG 
CCAAAAGAAG CAACAACAGC ATCTTCAGCA GCCCTGGCGG CTGCATCCAA ACCAACAGCA 
GCACCTTCCT CAGCCCCAGT AACAGCAACC AAACCAACAA AAACAAAGCC AAGTGCAGCA 
AAATCGCCTC CCAAACAAGA TATAAAGCCT ATCCCTGGGA AGCAGCAAAA AACAGACAGC 
AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT AAAGTTTCTC CAGCTGTGCT ACCTAAGATG 
ACACCTAGTT CTGCTGGTAA AGTATCTGCA GTTGCCACTG GAGCTCTAGC AGCTGCTGGT 
GCCGCCGCTG CAGTCACTGC TGTGCAGTCA ACAAAGCCGA AAGATGAGAG TAAAGAGGAA 
AAGAAAGCCA TTACTGGGGA TAAGGTTTCT TCTGGGCCAC CGGCCAAGAC AGACGCAGAT 
GCAGCCTTGG ACGAACTACT TGGAACGTTA GAGGGCCCAG CAGATATTCC AGAGTCTCCA 
CAATTTACTG GACCAGAAAT CCAGGACACA GTTGTTACAT CTGAGTATTT GGAGGAGCTA 
GGAAAACGGG ATTTCACCAT CCCACCTGAA TACAGGCACC TGCTGGATGG TAAAGGTGAA 
AAGATGGCAC CCCCTACCCC ACCCGAGGAA CCCGAAGAGC ACATGGATGA TGATGAATTA 
GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA 
AAGCTGGAAG AGAAAATGGA AGTGAAACCA AAATCAGCAG CAGCAGTGGC CACACAGCCA 
GATGTGCAGA AAAAAGAAGT AAAAGTCGCA GCAGCAGAAA CTTCCAAACC AAGCACTCAG 
AAAAAAGAGG AAAAAGCAAA AATAGCAGAA GTGTCTAAAC CAAGCACTCA GAAAAAAGAG 
GAAAAACTGG CAGCAGCAGC AGACACATCC AAACCAAGCA CTCAGAAAAA AGAGGTAAAA 
GCCACAGCAA AAGCCACAGC TGCCGAAGCT TCCAAACAAA GCACTGAGTC CATACCATCA 
GAAGCAATAG ACGAGTTGTT GGGAACACTT GAAGGACCCC CAGAAAATGT TCCAGGGTCT 
CCAGTATTTC AGGGTCCAGA AGTTACTGAG AACATTACAG CAACATACTT AGAAGAAACT 
GGAAAAAGAG ATAGTACGAT CCCTCCAGAA TACAGAAAAT TATTAGATGG AAAGGATCAT 
GGGAAAGATG TGTGGCCTTC AGCACCTGAA CCAGAACCGT CTATGACCGA CACAGAGCTT 
GTAGATGTGT TTTCCAAAGA CTTTGAGCTT TCATGTTCAC CTACTGTTCA GACCACGCCA 
GCCATTAAAC CCAAAAGGAA CACTGAAGAT CTCCACGAGT GCTATTGCTC AAAGTCAGTG 
AGCCAACTTT CATCATCTGA AACTCCTCAA GGTAACAAAA CGAAACCTGA AATTGCGGCA 
TCATCTGCAT CCACTGTAAA AGCAGCAGCG CCCTCCGGTG GTATGGACAG TGCATTAGAT 
GAATTATTGG GAACACTGGA AGGTCCTGAT CCAACAGCCC CTGAATCACC TGTGTACACT 
GGGCCAGAGG TGACGGAAAC AGAGACAGCG GTATACAGAG AGGGACTTGG GATAAGAGAC 
AGCAGCATCC CTCCAGCATA CAGGCACTTA CTAGATGGGA AAGTAGATGG AAAAGATGCA 
CCCCCACCAC CGCCAGAGGA AAAGCCTCTG ACCGATGATC AGCTGATTGA TAAGTTTTCT 
ATGGACTTTG ATTGTTCTGA ATCTCCTGCA ACACAACAGG CTCATCCTGT CGAACCTAAA 
GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA GAAGTAGTTG CTGCCTCCTC TGCTACTGTT 
AAAGCTGCAC CCACTCCTTC TAAAGCACCA GCACCTCCAG CTGATCCACT CGATAGCCTG 
TCTGGTACCT TAGGTGTTCG AAAGGAAGAA CCGAGTGACA AGAAGCCTCC AGTGGATAAA 
GTAAAGGAGG GAACAGGCAA AGAGAAGAAA GAAAAGCTTG GAGAAGATGA AAAAACAATA 
CATCCTGACC GCCGGCTAAA GGAAGTGAAG GATAAAGACG GAAAGCCCTT GCTGCCAAAA 
CTTGAACAAA GCCCTAAGCC AATGAGTGAG GCCGAGCTGC TGGATGCTTT GACTGAAGGC 
TTTGTGACTT CCCCTGCCAC TCCTGATGCC CCACTGCAGG GCTCTTTGAA AAAAAAAGGT 
TCTGGATCAG AAGATGTTAT ATCTTGTTCT AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA 
CAGGACGCTG CTTTAGATAT ACAGATTCCC GACGATGCAC TAGATTTACT TTCTGGCTCC 
CTGGGACAAA GAGAGGTGGA TCCTAATGAA AACAAACCGG TGGTGGATGT AGTTAAGGAA 
AAAGCTAAAG CTGAGCATAT AGACAAACTG GGGGACAGAG ATGATACAAT CCCACCGGAA 
TACAGAAAGC TGCTGGATGG TAAAGATGAT AAGGGACAAC CTGCAAAACC ACCAGCGAAA 
GCGGAGGAGA AACCAAAGAA GCCTTTAAGT GACGATGCAG CGATCGATGC TCTTTCAAGC 
GGATTTGCAT CATGTGACAC AAAATCTACT GAACAACCAA AGCAGCCTTC AACGGACAAG 
ACTGAGAAAC AATGTGCTAG CGCTCCAGTG TCGCAGCCTT CAGGAAAGGC ACCTGATGCT 
GCAAAGCAGG CAAACTCAGC GATACCAAGT GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956118.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811572.1 Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OXa31739
Clone ID Related Accession (Same CDS sequence)	XM_012956118.1
Accession Version	XM_012956118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG 
CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA 
GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG 
AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG 
ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT 
CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT 
TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT 
GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT 
GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA 
GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG 
GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT 
CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA 
AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG 
TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT 
GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC 
TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT 
GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC 
GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT 
CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC 
TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811572.1
CDS	206..3031
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956118.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956118.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG 
CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA 
GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG 
AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG 
ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT 
CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT 
TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT 
GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT 
GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA 
GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG 
GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT 
CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA 
AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG 
TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT 
GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC 
TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT 
GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC 
GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT 
CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC 
TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956125.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811579.1 Xenopus tropicalis calpastatin (cast), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OXa31740
Clone ID Related Accession (Same CDS sequence)	XM_012956125.1
Accession Version	XM_012956125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA 
GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA 
GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA 
GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG 
GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA 
GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT 
GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG 
CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC 
ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC 
CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA 
GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT 
TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA 
AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT 
CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC 
ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA 
GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA 
ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA 
TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT 
ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT 
ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC 
TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG 
AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT 
ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT 
GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG 
GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGGGAAA 
GTAGATGGAA AAGATGCACC CCCACCACCG CCAGAGGAAA AGCCTCTGAC CGATGATCAG 
CTGATTGATA AGTTTTCTAT GGACTTTGAT TGTTCTGAAT CTCCTGCAAC ACAACAGGCT 
CATCCTGTCG AACCTAAAGT TTCCTCGCAA GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT 
GCCTCCTCTG CTACTGTTAA AGCTGCACCC ACTCCTTCTA AAGCACCAGC ACCTCCAGCT 
GATCCACTCG ATAGCCTGTC TGGTACCTTA GGTGTTCGAA AGGAAGAACC GAGTGACAAG 
AAGCCTCCAG TGGATAAAGT AAAGGAGGGA ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA 
GAAGATGAAA AAACAATACA TCCTGACCGC CGGCTAAAGG AAGTGAAGGA TAAAGACGGA 
AAGCCCTTGC TGCCAAAACT TGAACAAAGC CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG 
GATGCTTTGA CTGAAGGCTT TGTGACTTCC CCTGCCACTC CTGATGCCCC ACTGCAGGGC 
TCTTTGAAAA AAAAAGGTTC TGGATCAGAA GATGTTATAT CTTGTTCTAA AGCTTCCGCC 
GTCAAGTCTG GAGCTCCACA GGACGCTGCT TTAGATATAC AGATTCCCGA CGATGCACTA 
GATTTACTTT CTGGCTCCCT GGGACAAAGA GAGGTGGATC CTAATGAAAA CAAACCGGTG 
GTGGATGTAG TTAAGGAAAA AGCTAAAGCT GAGCATATAG ACAAACTGGG GGACAGAGAT 
GATACAATCC CACCGGAATA CAGAAAGCTG CTGGATGGTA AAGATGATAA GGGACAACCT 
GCAAAACCAC CAGCGAAAGC GGAGGAGAAA CCAAAGAAGC CTTTAAGTGA CGATGCAGCG 
ATCGATGCTC TTTCAAGCGG ATTTGCATCA TGTGACACAA AATCTACTGA ACAACCAAAG 
CAGCCTTCAA CGGACAAGAC TGAGAAACAA TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA 
GGAAAGGCAC CTGATGCTGC AAAGCAGGCA AACTCAGCGA TACCAAGTGT GCCAAAGGCT 
GGCAAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811579.1
CDS	229..3000
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAETPSASGKTMNPADTQAKQTPKTEPAKSTKPTKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956125.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956125.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG GCTAAACAAA CACCCAAGAC TGAACCAGCC 
AAATCTACAA AGCCAACAAA GAGTAAAGAT GAAAAGAAAA GTGGTGATAA AGTTTCTCCA 
GCTGTGCTAC CTAAGATGAC ACCTAGTTCT GCTGGTAAAG TATCTGCAGT TGCCACTGGA 
GCTCTAGCAG CTGCTGGTGC CGCCGCTGCA GTCACTGCTG TGCAGTCAAC AAAGCCGAAA 
GATGAGAGTA AAGAGGAAAA GAAAGCCATT ACTGGGGATA AGGTTTCTTC TGGGCCACCG 
GCCAAGACAG ACGCAGATGC AGCCTTGGAC GAACTACTTG GAACGTTAGA GGGCCCAGCA 
GATATTCCAG AGTCTCCACA ATTTACTGGA CCAGAAATCC AGGACACAGT TGTTACATCT 
GAGTATTTGG AGGAGCTAGG AAAACGGGAT TTCACCATCC CACCTGAATA CAGGCACCTG 
CTGGATGGTA AAGGTGAAAA GATGGCACCC CCTACCCCAC CCGAGGAACC CGAAGAGCAC 
ATGGATGATG ATGAATTAGT TGCAGCCCTG TCTTCTGGTT TTGAGAGCAG TCAGGCATCC 
CCCGAAGTAA AGAAGCCAAA GCTGGAAGAG AAAATGGAAG TGAAACCAAA ATCAGCAGCA 
GCAGTGGCCA CACAGCCAGA TGTGCAGAAA AAAGAAGTAA AAGTCGCAGC AGCAGAAACT 
TCCAAACCAA GCACTCAGAA AAAAGAGGAA AAAGCAAAAA TAGCAGAAGT GTCTAAACCA 
AGCACTCAGA AAAAAGAGGA AAAACTGGCA GCAGCAGCAG ACACATCCAA ACCAAGCACT 
CAGAAAAAAG AGGTAAAAGC CACAGCAAAA GCCACAGCTG CCGAAGCTTC CAAACAAAGC 
ACTGAGTCCA TACCATCAGA AGCAATAGAC GAGTTGTTGG GAACACTTGA AGGACCCCCA 
GAAAATGTTC CAGGGTCTCC AGTATTTCAG GGTCCAGAAG TTACTGAGAA CATTACAGCA 
ACATACTTAG AAGAAACTGG AAAAAGAGAT AGTACGATCC CTCCAGAATA CAGAAAATTA 
TTAGATGGAA AGGATCATGG GAAAGATGTG TGGCCTTCAG CACCTGAACC AGAACCGTCT 
ATGACCGACA CAGAGCTTGT AGATGTGTTT TCCAAAGACT TTGAGCTTTC ATGTTCACCT 
ACTGTTCAGA CCACGCCAGC CATTAAACCC AAAAGGAACA CTGAAGATCT CCACGAGTGC 
TATTGCTCAA AGTCAGTGAG CCAACTTTCA TCATCTGAAA CTCCTCAAGG TAACAAAACG 
AAACCTGAAA TTGCGGCATC ATCTGCATCC ACTGTAAAAG CAGCAGCGCC CTCCGGTGGT 
ATGGACAGTG CATTAGATGA ATTATTGGGA ACACTGGAAG GTCCTGATCC AACAGCCCCT 
GAATCACCTG TGTACACTGG GCCAGAGGTG ACGGAAACAG AGACAGCGGT ATACAGAGAG 
GGACTTGGGA TAAGAGACAG CAGCATCCCT CCAGCATACA GGCACTTACT AGATGGGAAA 
GTAGATGGAA AAGATGCACC CCCACCACCG CCAGAGGAAA AGCCTCTGAC CGATGATCAG 
CTGATTGATA AGTTTTCTAT GGACTTTGAT TGTTCTGAAT CTCCTGCAAC ACAACAGGCT 
CATCCTGTCG AACCTAAAGT TTCCTCGCAA GAGAAAAAGG CAGAAAAAGA AGTAGTTGCT 
GCCTCCTCTG CTACTGTTAA AGCTGCACCC ACTCCTTCTA AAGCACCAGC ACCTCCAGCT 
GATCCACTCG ATAGCCTGTC TGGTACCTTA GGTGTTCGAA AGGAAGAACC GAGTGACAAG 
AAGCCTCCAG TGGATAAAGT AAAGGAGGGA ACAGGCAAAG AGAAGAAAGA AAAGCTTGGA 
GAAGATGAAA AAACAATACA TCCTGACCGC CGGCTAAAGG AAGTGAAGGA TAAAGACGGA 
AAGCCCTTGC TGCCAAAACT TGAACAAAGC CCTAAGCCAA TGAGTGAGGC CGAGCTGCTG 
GATGCTTTGA CTGAAGGCTT TGTGACTTCC CCTGCCACTC CTGATGCCCC ACTGCAGGGC 
TCTTTGAAAA AAAAAGGTTC TGGATCAGAA GATGTTATAT CTTGTTCTAA AGCTTCCGCC 
GTCAAGTCTG GAGCTCCACA GGACGCTGCT TTAGATATAC AGATTCCCGA CGATGCACTA 
GATTTACTTT CTGGCTCCCT GGGACAAAGA GAGGTGGATC CTAATGAAAA CAAACCGGTG 
GTGGATGTAG TTAAGGAAAA AGCTAAAGCT GAGCATATAG ACAAACTGGG GGACAGAGAT 
GATACAATCC CACCGGAATA CAGAAAGCTG CTGGATGGTA AAGATGATAA GGGACAACCT 
GCAAAACCAC CAGCGAAAGC GGAGGAGAAA CCAAAGAAGC CTTTAAGTGA CGATGCAGCG 
ATCGATGCTC TTTCAAGCGG ATTTGCATCA TGTGACACAA AATCTACTGA ACAACCAAAG 
CAGCCTTCAA CGGACAAGAC TGAGAAACAA TGTGCTAGCG CTCCAGTGTC GCAGCCTTCA 
GGAAAGGCAC CTGATGCTGC AAAGCAGGCA AACTCAGCGA TACCAAGTGT GCCAAAGGCT 
GGCAAAAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_012956133.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811587.1 Xenopus tropicalis calpastatin (cast), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OXa31741
Clone ID Related Accession (Same CDS sequence)	XM_012956133.1
Accession Version	XM_012956133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCGG CGGATACACA GGCTAAACAA ACACCCAAGA CTGAACCAGC CAAATCTACA 
AAGCCAACAA AGCCAAAAGA AGCAACAACA GCATCTTCAG CAGCCCTGGC GGCTGCATCC 
AAACCAACAG CAGCACCTTC CTCAGCCCCA GTAACAGCAA CCAAACCAAC AAAAACAAAG 
CCAAGTGCAG CAAAATCGCC TCCCAAACAA GATATAAAGC CTATCCCTGG GAAGCAGCAA 
AAAACAGACA GCAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG 
CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA 
GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG 
AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG 
ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT 
CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT 
TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT 
GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT 
GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA 
GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG 
GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT 
CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA 
AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG 
TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT 
GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC 
TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT 
GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC 
GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT 
CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC 
TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811587.1
CDS	167..2992
Translation	MNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_012956133.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956133.1

ORF Insert Sequence:

ATGAACCCGG CGGATACACA GGCTAAACAA ACACCCAAGA CTGAACCAGC CAAATCTACA 
AAGCCAACAA AGCCAAAAGA AGCAACAACA GCATCTTCAG CAGCCCTGGC GGCTGCATCC 
AAACCAACAG CAGCACCTTC CTCAGCCCCA GTAACAGCAA CCAAACCAAC AAAAACAAAG 
CCAAGTGCAG CAAAATCGCC TCCCAAACAA GATATAAAGC CTATCCCTGG GAAGCAGCAA 
AAAACAGACA GCAAGAGTAA AGATGAAAAG AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG 
CTACCTAAGA TGACACCTAG TTCTGCTGGT AAAGTATCTG CAGTTGCCAC TGGAGCTCTA 
GCAGCTGCTG GTGCCGCCGC TGCAGTCACT GCTGTGCAGT CAACAAAGCC GAAAGATGAG 
AGTAAAGAGG AAAAGAAAGC CATTACTGGG GATAAGGTTT CTTCTGGGCC ACCGGCCAAG 
ACAGACGCAG ATGCAGCCTT GGACGAACTA CTTGGAACGT TAGAGGGCCC AGCAGATATT 
CCAGAGTCTC CACAATTTAC TGGACCAGAA ATCCAGGACA CAGTTGTTAC ATCTGAGTAT 
TTGGAGGAGC TAGGAAAACG GGATTTCACC ATCCCACCTG AATACAGGCA CCTGCTGGAT 
GGTAAAGGTG AAAAGATGGC ACCCCCTACC CCACCCGAGG AACCCGAAGA GCACATGGAT 
GATGATGAAT TAGTTGCAGC CCTGTCTTCT GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA 
GTAAAGAAGC CAAAGCTGGA AGAGAAAATG GAAGTGAAAC CAAAATCAGC AGCAGCAGTG 
GCCACACAGC CAGATGTGCA GAAAAAAGAA GTAAAAGTCG CAGCAGCAGA AACTTCCAAA 
CCAAGCACTC AGAAAAAAGA GGAAAAAGCA AAAATAGCAG AAGTGTCTAA ACCAAGCACT 
CAGAAAAAAG AGGAAAAACT GGCAGCAGCA GCAGACACAT CCAAACCAAG CACTCAGAAA 
AAAGAGGTAA AAGCCACAGC AAAAGCCACA GCTGCCGAAG CTTCCAAACA AAGCACTGAG 
TCCATACCAT CAGAAGCAAT AGACGAGTTG TTGGGAACAC TTGAAGGACC CCCAGAAAAT 
GTTCCAGGGT CTCCAGTATT TCAGGGTCCA GAAGTTACTG AGAACATTAC AGCAACATAC 
TTAGAAGAAA CTGGAAAAAG AGATAGTACG ATCCCTCCAG AATACAGAAA ATTATTAGAT 
GGAAAGGATC ATGGGAAAGA TGTGTGGCCT TCAGCACCTG AACCAGAACC GTCTATGACC 
GACACAGAGC TTGTAGATGT GTTTTCCAAA GACTTTGAGC TTTCATGTTC ACCTACTGTT 
CAGACCACGC CAGCCATTAA ACCCAAAAGG AACACTGAAG ATCTCCACGA GTGCTATTGC 
TCAAAGTCAG TGAGCCAACT TTCATCATCT GAAACTCCTC AAGGTAACAA AACGAAACCT 
GAAATTGCGG CATCATCTGC ATCCACTGTA AAAGCAGCAG CGCCCTCCGG TGGTATGGAC 
AGTGCATTAG ATGAATTATT GGGAACACTG GAAGGTCCTG ATCCAACAGC CCCTGAATCA 
CCTGTGTACA CTGGGCCAGA GGTGACGGAA ACAGAGACAG CGGTATACAG AGAGGGACTT 
GGGATAAGAG ACAGCAGCAT CCCTCCAGCA TACAGGCACT TACTAGATGG GAAAGTAGAT 
GGAAAAGATG CACCCCCACC ACCGCCAGAG GAAAAGCCTC TGACCGATGA TCAGCTGATT 
GATAAGTTTT CTATGGACTT TGATTGTTCT GAATCTCCTG CAACACAACA GGCTCATCCT 
GTCGAACCTA AAGTTTCCTC GCAAGAGAAA AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC 
TCTGCTACTG TTAAAGCTGC ACCCACTCCT TCTAAAGCAC CAGCACCTCC AGCTGATCCA 
CTCGATAGCC TGTCTGGTAC CTTAGGTGTT CGAAAGGAAG AACCGAGTGA CAAGAAGCCT 
CCAGTGGATA AAGTAAAGGA GGGAACAGGC AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT 
GAAAAAACAA TACATCCTGA CCGCCGGCTA AAGGAAGTGA AGGATAAAGA CGGAAAGCCC 
TTGCTGCCAA AACTTGAACA AAGCCCTAAG CCAATGAGTG AGGCCGAGCT GCTGGATGCT 
TTGACTGAAG GCTTTGTGAC TTCCCCTGCC ACTCCTGATG CCCCACTGCA GGGCTCTTTG 
AAAAAAAAAG GTTCTGGATC AGAAGATGTT ATATCTTGTT CTAAAGCTTC CGCCGTCAAG 
TCTGGAGCTC CACAGGACGC TGCTTTAGAT ATACAGATTC CCGACGATGC ACTAGATTTA 
CTTTCTGGCT CCCTGGGACA AAGAGAGGTG GATCCTAATG AAAACAAACC GGTGGTGGAT 
GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT ATAGACAAAC TGGGGGACAG AGATGATACA 
ATCCCACCGG AATACAGAAA GCTGCTGGAT GGTAAAGATG ATAAGGGACA ACCTGCAAAA 
CCACCAGCGA AAGCGGAGGA GAAACCAAAG AAGCCTTTAA GTGACGATGC AGCGATCGAT 
GCTCTTTCAA GCGGATTTGC ATCATGTGAC ACAAAATCTA CTGAACAACC AAAGCAGCCT 
TCAACGGACA AGACTGAGAA ACAATGTGCT AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG 
GCACCTGATG CTGCAAAGCA GGCAAACTCA GCGATACCAA GTGTGCCAAA GGCTGGCAAA 
AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_018090942.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946431.1 Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa41680
Clone ID Related Accession (Same CDS sequence)	XM_018090942.1
Accession Version	XM_018090942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946431.1
CDS	206..3166
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQAKQTPKTEPAKSTKPTKPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_018090942.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090942.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGGCTAAA CAAACACCCA AGACTGAACC AGCCAAATCT 
ACAAAGCCAA CAAAGCCAAA AGAAGCAACA ACAGCATCTT CAGCAGCCCT GGCGGCTGCA 
TCCAAACCAA CAGCAGCACC TTCCTCAGCC CCAGTAACAG CAACCAAACC AACAAAAACA 
AAGCCAAGTG CAGCAAAATC GCCTCCCAAA CAAGATATAA AGCCTATCCC TGGGAAGCAG 
CAAAAAACAG ACAGCAAGAG TAAAGATGAA AAGAAAAGTG GTGATAAAGT TTCTCCAGCT 
GTGCTACCTA AGATGACACC TAGTTCTGCT GGTAAAGTAT CTGCAGTTGC CACTGGAGCT 
CTAGCAGCTG CTGGTGCCGC CGCTGCAGTC ACTGCTGTGC AGTCAACAAA GCCGAAAGAT 
GAGAGTAAAG AGGAAAAGAA AGCCATTACT GGGGATAAGG TTTCTTCTGG GCCACCGGCC 
AAGACAGACG CAGATGCAGC CTTGGACGAA CTACTTGGAA CGTTAGAGGG CCCAGCAGAT 
ATTCCAGAGT CTCCACAATT TACTGGACCA GAAATCCAGG ACACAGTTGT TACATCTGAG 
TATTTGGAGG AGCTAGGAAA ACGGGATTTC ACCATCCCAC CTGAATACAG GCACCTGCTG 
GATGGTAAAG GTGAAAAGAT GGCACCCCCT ACCCCACCCG AGGAACCCGA AGAGCACATG 
GATGATGATG AATTAGTTGC AGCCCTGTCT TCTGGTTTTG AGAGCAGTCA GGCATCCCCC 
GAAGTAAAGA AGCCAAAGCT GGAAGAGAAA ATGGAAGTGA AACCAAAATC AGCAGCAGCA 
GTGGCCACAC AGCCAGATGT GCAGAAAAAA GAAGTAAAAG TCGCAGCAGC AGAAACTTCC 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA GCAAAAATAG CAGAAGTGTC TAAACCAAGC 
ACTCAGAAAA AAGAGGAAAA ACTGGCAGCA GCAGCAGACA CATCCAAACC AAGCACTCAG 
AAAAAAGAGG TAAAAGCCAC AGCAAAAGCC ACAGCTGCCG AAGCTTCCAA ACAAAGCACT 
GAGTCCATAC CATCAGAAGC AATAGACGAG TTGTTGGGAA CACTTGAAGG ACCCCCAGAA 
AATGTTCCAG GGTCTCCAGT ATTTCAGGGT CCAGAAGTTA CTGAGAACAT TACAGCAACA 
TACTTAGAAG AAACTGGAAA AAGAGATAGT ACGATCCCTC CAGAATACAG AAAATTATTA 
GATGGAAAGG ATCATGGGAA AGATGTGTGG CCTTCAGCAC CTGAACCAGA ACCGTCTATG 
ACCGACACAG AGCTTGTAGA TGTGTTTTCC AAAGACTTTG AGCTTTCATG TTCACCTACT 
GTTCAGACCA CGCCAGCCAT TAAACCCAAA AGGAACACTG AAGATCTCCA CGAGTGCTAT 
TGCTCAAAGT CAGTGAGCCA ACTTTCATCA TCTGAAACTC CTCAAGGTAA CAAAACGAAA 
CCTGAAATTG CGGCATCATC TGCATCCACT GTAAAAGCAG CAGCGCCCTC CGGTGGTATG 
GACAGTGCAT TAGATGAATT ATTGGGAACA CTGGAAGGTC CTGATCCAAC AGCCCCTGAA 
TCACCTGTGT ACACTGGGCC AGAGGTGACG GAAACAGAGA CAGCGGTATA CAGAGAGGGA 
CTTGGGATAA GAGACAGCAG CATCCCTCCA GCATACAGGC ACTTACTAGA TGGGAAAGTA 
GATGGAAAAG ATGCACCCCC ACCACCGCCA GAGGAAAAGC CTCTGACCGA TGATCAGCTG 
ATTGATAAGT TTTCTATGGA CTTTGATTGT TCTGAATCTC CTGCAACACA ACAGGCTCAT 
CCTGTCGAAC CTAAAGTTTC CTCGCAAGAG AAAAAGGCAG AAAAAGAAGT AGTTGCTGCC 
TCCTCTGCTA CTGTTAAAGC TGCACCCACT CCTTCTAAAG CACCAGCACC TCCAGCTGAT 
CCACTCGATA GCCTGTCTGG TACCTTAGGT GTTCGAAAGG AAGAACCGAG TGACAAGAAG 
CCTCCAGTGG ATAAAGTAAA GGAGGGAACA GGCAAAGAGA AGAAAGAAAA GCTTGGAGAA 
GATGAAAAAA CAATACATCC TGACCGCCGG CTAAAGGAAG TGAAGCCAAT GAGTGAGGCC 
GAGCTGCTGG ATGCTTTGAC TGAAGGCTTT GTGACTTCCC CTGCCACTCC TGATGCCCCA 
CTGCAGGGCT CTTTGAAAAA AAAAGGTTCT GGATCAGAAG ATGTTATATC TTGTTCTAAA 
GCTTCCGCCG TCAAGTCTGG AGCTCCACAG GACGCTGCTT TAGATATACA GATTCCCGAC 
GATGCACTAG ATTTACTTTC TGGCTCCCTG GGACAAAGAG AGGTGGATCC TAATGAAAAC 
AAACCGGTGG TGGATGTAGT TAAGGAAAAA GCTAAAGCTG AGCATATAGA CAAACTGGGG 
GACAGAGATG ATACAATCCC ACCGGAATAC AGAAAGCTGC TGGATGGTAA AGATGATAAG 
GGACAACCTG CAAAACCACC AGCGAAAGCG GAGGAGAAAC CAAAGAAGCC TTTAAGTGAC 
GATGCAGCGA TCGATGCTCT TTCAAGCGGA TTTGCATCAT GTGACACAAA ATCTACTGAA 
CAACCAAAGC AGCCTTCAAC GGACAAGACT GAGAAACAAT GTGCTAGCGC TCCAGTGTCG 
CAGCCTTCAG GAAAGGCACC TGATGCTGCA AAGCAGGCAA ACTCAGCGAT ACCAAGTGTG 
CCAAAGGCTG GCAAAAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_018090946.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946435.1 Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OXa41681
Clone ID Related Accession (Same CDS sequence)	XM_018090946.1
Accession Version	XM_018090946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGCCAAAA GAAGCAACAA CAGCATCTTC AGCAGCCCTG 
GCGGCTGCAT CCAAACCAAC AGCAGCACCT TCCTCAGCCC CAGTAACAGC AACCAAACCA 
ACAAAAACAA AGCCAAGTGC AGCAAAATCG CCTCCCAAAC AAGATATAAA GCCTATCCCT 
GGGAAGCAGC AAAAAACAGA CAGCAAGAGT AAAGATGAAA AGAAAAGTGG TGATAAAGTT 
TCTCCAGCTG TGCTACCTAA GATGACACCT AGTTCTGCTG GTAAAGTATC TGCAGTTGCC 
ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC GCTGCAGTCA CTGCTGTGCA GTCAACAAAG 
CCGAAAGATG AGAGTAAAGA GGAAAAGAAA GCCATTACTG GGGATAAGGT TTCTTCTGGG 
CCACCGGCCA AGACAGACGC AGATGCAGCC TTGGACGAAC TACTTGGAAC GTTAGAGGGC 
CCAGCAGATA TTCCAGAGTC TCCACAATTT ACTGGACCAG AAATCCAGGA CACAGTTGTT 
ACATCTGAGT ATTTGGAGGA GCTAGGAAAA CGGGATTTCA CCATCCCACC TGAATACAGG 
CACCTGCTGG ATGGTAAAGG TGAAAAGATG GCACCCCCTA CCCCACCCGA GGAACCCGAA 
GAGCACATGG ATGATGATGA ATTAGTTGCA GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG 
GCATCCCCCG AAGTAAAGAA GCCAAAGCTG GAAGAGAAAA TGGAAGTGAA ACCAAAATCA 
GCAGCAGCAG TGGCCACACA GCCAGATGTG CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA 
GAAACTTCCA AACCAAGCAC TCAGAAAAAA GAGGAAAAAG CAAAAATAGC AGAAGTGTCT 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA 
AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA 
CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA 
CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT 
ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA 
AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA 
CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT 
TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC 
GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC 
AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC 
GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA 
GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC 
AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT 
GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946435.1
CDS	206..3163
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAEKKTGASGPSGAQASGTSKTPSASGKTMNPADTQPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_018090946.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090946.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGAAGAA AACTGGTGCC 
AGTGGCCCTT CAGGGGCCCA AGCAAGTGGG ACCAGCAAGA CTCCTAGTGC TTCAGGAAAA 
ACTATGAACC CGGCGGATAC ACAGCCAAAA GAAGCAACAA CAGCATCTTC AGCAGCCCTG 
GCGGCTGCAT CCAAACCAAC AGCAGCACCT TCCTCAGCCC CAGTAACAGC AACCAAACCA 
ACAAAAACAA AGCCAAGTGC AGCAAAATCG CCTCCCAAAC AAGATATAAA GCCTATCCCT 
GGGAAGCAGC AAAAAACAGA CAGCAAGAGT AAAGATGAAA AGAAAAGTGG TGATAAAGTT 
TCTCCAGCTG TGCTACCTAA GATGACACCT AGTTCTGCTG GTAAAGTATC TGCAGTTGCC 
ACTGGAGCTC TAGCAGCTGC TGGTGCCGCC GCTGCAGTCA CTGCTGTGCA GTCAACAAAG 
CCGAAAGATG AGAGTAAAGA GGAAAAGAAA GCCATTACTG GGGATAAGGT TTCTTCTGGG 
CCACCGGCCA AGACAGACGC AGATGCAGCC TTGGACGAAC TACTTGGAAC GTTAGAGGGC 
CCAGCAGATA TTCCAGAGTC TCCACAATTT ACTGGACCAG AAATCCAGGA CACAGTTGTT 
ACATCTGAGT ATTTGGAGGA GCTAGGAAAA CGGGATTTCA CCATCCCACC TGAATACAGG 
CACCTGCTGG ATGGTAAAGG TGAAAAGATG GCACCCCCTA CCCCACCCGA GGAACCCGAA 
GAGCACATGG ATGATGATGA ATTAGTTGCA GCCCTGTCTT CTGGTTTTGA GAGCAGTCAG 
GCATCCCCCG AAGTAAAGAA GCCAAAGCTG GAAGAGAAAA TGGAAGTGAA ACCAAAATCA 
GCAGCAGCAG TGGCCACACA GCCAGATGTG CAGAAAAAAG AAGTAAAAGT CGCAGCAGCA 
GAAACTTCCA AACCAAGCAC TCAGAAAAAA GAGGAAAAAG CAAAAATAGC AGAAGTGTCT 
AAACCAAGCA CTCAGAAAAA AGAGGAAAAA CTGGCAGCAG CAGCAGACAC ATCCAAACCA 
AGCACTCAGA AAAAAGAGGT AAAAGCCACA GCAAAAGCCA CAGCTGCCGA AGCTTCCAAA 
CAAAGCACTG AGTCCATACC ATCAGAAGCA ATAGACGAGT TGTTGGGAAC ACTTGAAGGA 
CCCCCAGAAA ATGTTCCAGG GTCTCCAGTA TTTCAGGGTC CAGAAGTTAC TGAGAACATT 
ACAGCAACAT ACTTAGAAGA AACTGGAAAA AGAGATAGTA CGATCCCTCC AGAATACAGA 
AAATTATTAG ATGGAAAGGA TCATGGGAAA GATGTGTGGC CTTCAGCACC TGAACCAGAA 
CCGTCTATGA CCGACACAGA GCTTGTAGAT GTGTTTTCCA AAGACTTTGA GCTTTCATGT 
TCACCTACTG TTCAGACCAC GCCAGCCATT AAACCCAAAA GGAACACTGA AGATCTCCAC 
GAGTGCTATT GCTCAAAGTC AGTGAGCCAA CTTTCATCAT CTGAAACTCC TCAAGGTAAC 
AAAACGAAAC CTGAAATTGC GGCATCATCT GCATCCACTG TAAAAGCAGC AGCGCCCTCC 
GGTGGTATGG ACAGTGCATT AGATGAATTA TTGGGAACAC TGGAAGGTCC TGATCCAACA 
GCCCCTGAAT CACCTGTGTA CACTGGGCCA GAGGTGACGG AAACAGAGAC AGCGGTATAC 
AGAGAGGGAC TTGGGATAAG AGACAGCAGC ATCCCTCCAG CATACAGGCA CTTACTAGAT 
GGGAAAGTAG ATGGAAAAGA TGCACCCCCA CCACCGCCAG AGGAAAAGCC TCTGACCGAT 
GATCAGCTGA TTGATAAGTT TTCTATGGAC TTTGATTGTT CTGAATCTCC TGCAACACAA 
CAGGCTCATC CTGTCGAACC TAAAGTTTCC TCGCAAGAGA AAAAGGCAGA AAAAGAAGTA 
GTTGCTGCCT CCTCTGCTAC TGTTAAAGCT GCACCCACTC CTTCTAAAGC ACCAGCACCT 
CCAGCTGATC CACTCGATAG CCTGTCTGGT ACCTTAGGTG TTCGAAAGGA AGAACCGAGT 
GACAAGAAGC CTCCAGTGGA TAAAGTAAAG GAGGGAACAG GCAAAGAGAA GAAAGAAAAG 
CTTGGAGAAG ATGAAAAAAC AATACATCCT GACCGCCGGC TAAAGGAAGT GAAGGATAAA 
GACGGAAAGC CCTTGCTGCC AAAACTTGAA CAAAGCCCTA AGCCAATGAG TGAGGCCGAG 
CTGCTGGATG CTTTGACTGA AGGCTTTGTG ACTTCCCCTG CCACTCCTGA TGCCCCACTG 
CAGGGCTCTT TGAAAAAAAA AGGTTCTGGA TCAGAAGATG TTATATCTTG TTCTAAAGCT 
TCCGCCGTCA AGTCTGGAGC TCCACAGGAC GCTGCTTTAG ATATACAGAT TCCCGACGAT 
GCACTAGATT TACTTTCTGG CTCCCTGGGA CAAAGAGAGG TGGATCCTAA TGAAAACAAA 
CCGGTGGTGG ATGTAGTTAA GGAAAAAGCT AAAGCTGAGC ATATAGACAA ACTGGGGGAC 
AGAGATGATA CAATCCCACC GGAATACAGA AAGCTGCTGG ATGGTAAAGA TGATAAGGGA 
CAACCTGCAA AACCACCAGC GAAAGCGGAG GAGAAACCAA AGAAGCCTTT AAGTGACGAT 
GCAGCGATCG ATGCTCTTTC AAGCGGATTT GCATCATGTG ACACAAAATC TACTGAACAA 
CCAAAGCAGC CTTCAACGGA CAAGACTGAG AAACAATGTG CTAGCGCTCC AGTGTCGCAG 
CCTTCAGGAA AGGCACCTGA TGCTGCAAAG CAGGCAAACT CAGCGATACC AAGTGTGCCA 
AAGGCTGGCA AAAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_018090966.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946455.1 Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OXa41682
Clone ID Related Accession (Same CDS sequence)	XM_018090966.1
Accession Version	XM_018090966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG CCAAAAGAAG CAACAACAGC ATCTTCAGCA 
GCCCTGGCGG CTGCATCCAA ACCAACAGCA GCACCTTCCT CAGCCCCAGT AACAGCAACC 
AAACCAACAA AAACAAAGCC AAGTGCAGCA AAATCGCCTC CCAAACAAGA TATAAAGCCT 
ATCCCTGGGA AGCAGCAAAA AACAGACAGC AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT 
AAAGTTTCTC CAGCTGTGCT ACCTAAGATG ACACCTAGTT CTGCTGGTAA AGTATCTGCA 
GTTGCCACTG GAGCTCTAGC AGCTGCTGGT GCCGCCGCTG CAGTCACTGC TGTGCAGTCA 
ACAAAGCCGA AAGATGAGAG TAAAGAGGAA AAGAAAGCCA TTACTGGGGA TAAGGTTTCT 
TCTGGGCCAC CGGCCAAGAC AGACGCAGAT GCAGCCTTGG ACGAACTACT TGGAACGTTA 
GAGGGCCCAG CAGATATTCC AGAGTCTCCA CAATTTACTG GACCAGAAAT CCAGGACACA 
GTTGTTACAT CTGAGTATTT GGAGGAGCTA GGAAAACGGG ATTTCACCAT CCCACCTGAA 
TACAGGCACC TGCTGGATGG TAAAGGTGAA AAGATGGCAC CCCCTACCCC ACCCGAGGAA 
CCCGAAGAGC ACATGGATGA TGATGAATTA GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC 
AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA AAGCTGGAAG AGAAAATGGA AGTGAAACCA 
AAATCAGCAG CAGCAGTGGC CACACAGCCA GATGTGCAGA AAAAAGAAGT AAAAGTCGCA 
GCAGCAGAAA CTTCCAAACC AAGCACTCAG AAAAAAGAGG AAAAAGCAAA AATAGCAGAA 
GTGTCTAAAC CAAGCACTCA GAAAAAAGAG GAAAAACTGG CAGCAGCAGC AGACACATCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT 
CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA 
GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG 
CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT 
CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG 
GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA 
CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG 
ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA 
ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA 
GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA 
GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA 
CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA 
GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG 
GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG 
GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC 
CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT 
AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC 
GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA 
AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG 
GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT 
AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT 
GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT 
GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG 
TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT 
GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946455.1
CDS	206..3109
Translation	MSKPGKGRKHSGPGKGTKEQCDRKPADKKPSEGAETPSASGKTMNPADTQPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_018090966.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090966.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGCAA 
TGTGATAGGA AGCCTGCAGA CAAGAAGCCA TCAGAAGGAG CAGAGACTCC TAGTGCTTCA 
GGAAAAACTA TGAACCCGGC GGATACACAG CCAAAAGAAG CAACAACAGC ATCTTCAGCA 
GCCCTGGCGG CTGCATCCAA ACCAACAGCA GCACCTTCCT CAGCCCCAGT AACAGCAACC 
AAACCAACAA AAACAAAGCC AAGTGCAGCA AAATCGCCTC CCAAACAAGA TATAAAGCCT 
ATCCCTGGGA AGCAGCAAAA AACAGACAGC AAGAGTAAAG ATGAAAAGAA AAGTGGTGAT 
AAAGTTTCTC CAGCTGTGCT ACCTAAGATG ACACCTAGTT CTGCTGGTAA AGTATCTGCA 
GTTGCCACTG GAGCTCTAGC AGCTGCTGGT GCCGCCGCTG CAGTCACTGC TGTGCAGTCA 
ACAAAGCCGA AAGATGAGAG TAAAGAGGAA AAGAAAGCCA TTACTGGGGA TAAGGTTTCT 
TCTGGGCCAC CGGCCAAGAC AGACGCAGAT GCAGCCTTGG ACGAACTACT TGGAACGTTA 
GAGGGCCCAG CAGATATTCC AGAGTCTCCA CAATTTACTG GACCAGAAAT CCAGGACACA 
GTTGTTACAT CTGAGTATTT GGAGGAGCTA GGAAAACGGG ATTTCACCAT CCCACCTGAA 
TACAGGCACC TGCTGGATGG TAAAGGTGAA AAGATGGCAC CCCCTACCCC ACCCGAGGAA 
CCCGAAGAGC ACATGGATGA TGATGAATTA GTTGCAGCCC TGTCTTCTGG TTTTGAGAGC 
AGTCAGGCAT CCCCCGAAGT AAAGAAGCCA AAGCTGGAAG AGAAAATGGA AGTGAAACCA 
AAATCAGCAG CAGCAGTGGC CACACAGCCA GATGTGCAGA AAAAAGAAGT AAAAGTCGCA 
GCAGCAGAAA CTTCCAAACC AAGCACTCAG AAAAAAGAGG AAAAAGCAAA AATAGCAGAA 
GTGTCTAAAC CAAGCACTCA GAAAAAAGAG GAAAAACTGG CAGCAGCAGC AGACACATCC 
AAACCAAGCA CTCAGAAAAA AGAGGTAAAA GCCACAGCAA AAGCCACAGC TGCCGAAGCT 
TCCAAACAAA GCACTGAGTC CATACCATCA GAAGCAATAG ACGAGTTGTT GGGAACACTT 
GAAGGACCCC CAGAAAATGT TCCAGGGTCT CCAGTATTTC AGGGTCCAGA AGTTACTGAG 
AACATTACAG CAACATACTT AGAAGAAACT GGAAAAAGAG ATAGTACGAT CCCTCCAGAA 
TACAGAAAAT TATTAGATGG AAAGGATCAT GGGAAAGATG TGTGGCCTTC AGCACCTGAA 
CCAGAACCGT CTATGACCGA CACAGAGCTT GTAGATGTGT TTTCCAAAGA CTTTGAGCTT 
TCATGTTCAC CTACTGTTCA GACCACGCCA GCCATTAAAC CCAAAAGGAA CACTGAAGAT 
CTCCACGAGT GCTATTGCTC AAAGTCAGTG AGCCAACTTT CATCATCTGA AACTCCTCAA 
GGTAACAAAA CGAAACCTGA AATTGCGGCA TCATCTGCAT CCACTGTAAA AGCAGCAGCG 
CCCTCCGGTG GTATGGACAG TGCATTAGAT GAATTATTGG GAACACTGGA AGGTCCTGAT 
CCAACAGCCC CTGAATCACC TGTGTACACT GGGCCAGAGG TGACGGAAAC AGAGACAGCG 
GTATACAGAG AGGGACTTGG GATAAGAGAC AGCAGCATCC CTCCAGCATA CAGGCACTTA 
CTAGATGGGA AAGTAGATGG AAAAGATGCA CCCCCACCAC CGCCAGAGGA AAAGCCTCTG 
ACCGATGATC AGCTGATTGA TAAGTTTTCT ATGGACTTTG ATTGTTCTGA ATCTCCTGCA 
ACACAACAGG CTCATCCTGT CGAACCTAAA GTTTCCTCGC AAGAGAAAAA GGCAGAAAAA 
GAAGTAGTTG CTGCCTCCTC TGCTACTGTT AAAGCTGCAC CCACTCCTTC TAAAGCACCA 
GCACCTCCAG CTGATCCACT CGATAGCCTG TCTGGTACCT TAGGTGTTCG AAAGGAAGAA 
CCGAGTGACA AGAAGCCTCC AGTGGATAAA GTAAAGGAGG GAACAGGCAA AGAGAAGAAA 
GAAAAGCTTG GAGAAGATGA AAAAACAATA CATCCTGACC GCCGGCTAAA GGAAGTGAAG 
GATAAAGACG GAAAGCCCTT GCTGCCAAAA CTTGAACAAA GCCCTAAGCC AATGAGTGAG 
GCCGAGCTGC TGGATGCTTT GACTGAAGGC TTTGTGACTT CCCCTGCCAC TCCTGATGCC 
CCACTGCAGG GCTCTTTGAA AAAAAAAGGT TCTGGATCAG AAGATGTTAT ATCTTGTTCT 
AAAGCTTCCG CCGTCAAGTC TGGAGCTCCA CAGGACGCTG CTTTAGATAT ACAGATTCCC 
GACGATGCAC TAGATTTACT TTCTGGCTCC CTGGGACAAA GAGAGGTGGA TCCTAATGAA 
AACAAACCGG TGGTGGATGT AGTTAAGGAA AAAGCTAAAG CTGAGCATAT AGACAAACTG 
GGGGACAGAG ATGATACAAT CCCACCGGAA TACAGAAAGC TGCTGGATGG TAAAGATGAT 
AAGGGACAAC CTGCAAAACC ACCAGCGAAA GCGGAGGAGA AACCAAAGAA GCCTTTAAGT 
GACGATGCAG CGATCGATGC TCTTTCAAGC GGATTTGCAT CATGTGACAC AAAATCTACT 
GAACAACCAA AGCAGCCTTC AACGGACAAG ACTGAGAAAC AATGTGCTAG CGCTCCAGTG 
TCGCAGCCTT CAGGAAAGGC ACCTGATGCT GCAAAGCAGG CAAACTCAGC GATACCAAGT 
GTGCCAAAGG CTGGCAAAAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cast ( XM_018090967.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946456.1 Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.

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CloneID	OXa41683
Clone ID Related Accession (Same CDS sequence)	XM_018090967.1
Accession Version	XM_018090967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calpastatin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGCCAAAAGA AGCAACAACA 
GCATCTTCAG CAGCCCTGGC GGCTGCATCC AAACCAACAG CAGCACCTTC CTCAGCCCCA 
GTAACAGCAA CCAAACCAAC AAAAACAAAG CCAAGTGCAG CAAAATCGCC TCCCAAACAA 
GATATAAAGC CTATCCCTGG GAAGCAGCAA AAAACAGACA GCAAGAGTAA AGATGAAAAG 
AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG CTACCTAAGA TGACACCTAG TTCTGCTGGT 
AAAGTATCTG CAGTTGCCAC TGGAGCTCTA GCAGCTGCTG GTGCCGCCGC TGCAGTCACT 
GCTGTGCAGT CAACAAAGCC GAAAGATGAG AGTAAAGAGG AAAAGAAAGC CATTACTGGG 
GATAAGGTTT CTTCTGGGCC ACCGGCCAAG ACAGACGCAG ATGCAGCCTT GGACGAACTA 
CTTGGAACGT TAGAGGGCCC AGCAGATATT CCAGAGTCTC CACAATTTAC TGGACCAGAA 
ATCCAGGACA CAGTTGTTAC ATCTGAGTAT TTGGAGGAGC TAGGAAAACG GGATTTCACC 
ATCCCACCTG AATACAGGCA CCTGCTGGAT GGTAAAGGTG AAAAGATGGC ACCCCCTACC 
CCACCCGAGG AACCCGAAGA GCACATGGAT GATGATGAAT TAGTTGCAGC CCTGTCTTCT 
GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA GTAAAGAAGC CAAAGCTGGA AGAGAAAATG 
GAAGTGAAAC CAAAATCAGC AGCAGCAGTG GCCACACAGC CAGATGTGCA GAAAAAAGAA 
GTAAAAGTCG CAGCAGCAGA AACTTCCAAA CCAAGCACTC AGAAAAAAGA GGAAAAAGCA 
AAAATAGCAG AAGTGTCTAA ACCAAGCACT CAGAAAAAAG AGGAAAAACT GGCAGCAGCA 
GCAGACACAT CCAAACCAAG CACTCAGAAA AAAGAGGTAA AAGCCACAGC AAAAGCCACA 
GCTGCCGAAG CTTCCAAACA AAGCACTGAG TCCATACCAT CAGAAGCAAT AGACGAGTTG 
TTGGGAACAC TTGAAGGACC CCCAGAAAAT GTTCCAGGGT CTCCAGTATT TCAGGGTCCA 
GAAGTTACTG AGAACATTAC AGCAACATAC TTAGAAGAAA CTGGAAAAAG AGATAGTACG 
ATCCCTCCAG AATACAGAAA ATTATTAGAT GGAAAGGATC ATGGGAAAGA TGTGTGGCCT 
TCAGCACCTG AACCAGAACC GTCTATGACC GACACAGAGC TTGTAGATGT GTTTTCCAAA 
GACTTTGAGC TTTCATGTTC ACCTACTGTT CAGACCACGC CAGCCATTAA ACCCAAAAGG 
AACACTGAAG ATCTCCACGA GTGCTATTGC TCAAAGTCAG TGAGCCAACT TTCATCATCT 
GAAACTCCTC AAGGTAACAA AACGAAACCT GAAATTGCGG CATCATCTGC ATCCACTGTA 
AAAGCAGCAG CGCCCTCCGG TGGTATGGAC AGTGCATTAG ATGAATTATT GGGAACACTG 
GAAGGTCCTG ATCCAACAGC CCCTGAATCA CCTGTGTACA CTGGGCCAGA GGTGACGGAA 
ACAGAGACAG CGGTATACAG AGAGGGACTT GGGATAAGAG ACAGCAGCAT CCCTCCAGCA 
TACAGGCACT TACTAGATGG GAAAGTAGAT GGAAAAGATG CACCCCCACC ACCGCCAGAG 
GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT 
GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA 
AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT 
TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT 
CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC 
AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA 
AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG 
CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC 
ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT 
ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT 
ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG 
GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT 
ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT 
GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG 
AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC 
ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT 
AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA 
GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946456.1
CDS	206..3061
Translation	MSKPGKGRKHSGPGKGTKETPSASGKTMNPADTQPKEATTASSAALAAASKPTAAPSSAPVTATKPTKTKPSAAKSPPKQDIKPIPGKQQKTDSKSKDEKKSGDKVSPAVLPKMTPSSAGKVSAVATGALAAAGAAAAVTAVQSTKPKDESKEEKKAITGDKVSSGPPAKTDADAALDELLGTLEGPADIPESPQFTGPEIQDTVVTSEYLEELGKRDFTIPPEYRHLLDGKGEKMAPPTPPEEPEEHMDDDELVAALSSGFESSQASPEVKKPKLEEKMEVKPKSAAAVATQPDVQKKEVKVAAAETSKPSTQKKEEKAKIAEVSKPSTQKKEEKLAAAADTSKPSTQKKEVKATAKATAAEASKQSTESIPSEAIDELLGTLEGPPENVPGSPVFQGPEVTENITATYLEETGKRDSTIPPEYRKLLDGKDHGKDVWPSAPEPEPSMTDTELVDVFSKDFELSCSPTVQTTPAIKPKRNTEDLHECYCSKSVSQLSSSETPQGNKTKPEIAASSASTVKAAAPSGGMDSALDELLGTLEGPDPTAPESPVYTGPEVTETETAVYREGLGIRDSSIPPAYRHLLDGKVDGKDAPPPPPEEKPLTDDQLIDKFSMDFDCSESPATQQAHPVEPKVSSQEKKAEKEVVAASSATVKAAPTPSKAPAPPADPLDSLSGTLGVRKEEPSDKKPPVDKVKEGTGKEKKEKLGEDEKTIHPDRRLKEVKDKDGKPLLPKLEQSPKPMSEAELLDALTEGFVTSPATPDAPLQGSLKKKGSGSEDVISCSKASAVKSGAPQDAALDIQIPDDALDLLSGSLGQREVDPNENKPVVDVVKEKAKAEHIDKLGDRDDTIPPEYRKLLDGKDDKGQPAKPPAKAEEKPKKPLSDDAAIDALSSGFASCDTKSTEQPKQPSTDKTEKQCASAPVSQPSGKAPDAAKQANSAIPSVPKAGKS

Target ORF information:

RefSeq Version	XM_018090967.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calpastatin (cast), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090967.1

ORF Insert Sequence:

ATGTCAAAGC CGGGCAAAGG GAGGAAGCAC TCGGGGCCAG GCAAGGGCAC TAAAGAGACT 
CCTAGTGCTT CAGGAAAAAC TATGAACCCG GCGGATACAC AGCCAAAAGA AGCAACAACA 
GCATCTTCAG CAGCCCTGGC GGCTGCATCC AAACCAACAG CAGCACCTTC CTCAGCCCCA 
GTAACAGCAA CCAAACCAAC AAAAACAAAG CCAAGTGCAG CAAAATCGCC TCCCAAACAA 
GATATAAAGC CTATCCCTGG GAAGCAGCAA AAAACAGACA GCAAGAGTAA AGATGAAAAG 
AAAAGTGGTG ATAAAGTTTC TCCAGCTGTG CTACCTAAGA TGACACCTAG TTCTGCTGGT 
AAAGTATCTG CAGTTGCCAC TGGAGCTCTA GCAGCTGCTG GTGCCGCCGC TGCAGTCACT 
GCTGTGCAGT CAACAAAGCC GAAAGATGAG AGTAAAGAGG AAAAGAAAGC CATTACTGGG 
GATAAGGTTT CTTCTGGGCC ACCGGCCAAG ACAGACGCAG ATGCAGCCTT GGACGAACTA 
CTTGGAACGT TAGAGGGCCC AGCAGATATT CCAGAGTCTC CACAATTTAC TGGACCAGAA 
ATCCAGGACA CAGTTGTTAC ATCTGAGTAT TTGGAGGAGC TAGGAAAACG GGATTTCACC 
ATCCCACCTG AATACAGGCA CCTGCTGGAT GGTAAAGGTG AAAAGATGGC ACCCCCTACC 
CCACCCGAGG AACCCGAAGA GCACATGGAT GATGATGAAT TAGTTGCAGC CCTGTCTTCT 
GGTTTTGAGA GCAGTCAGGC ATCCCCCGAA GTAAAGAAGC CAAAGCTGGA AGAGAAAATG 
GAAGTGAAAC CAAAATCAGC AGCAGCAGTG GCCACACAGC CAGATGTGCA GAAAAAAGAA 
GTAAAAGTCG CAGCAGCAGA AACTTCCAAA CCAAGCACTC AGAAAAAAGA GGAAAAAGCA 
AAAATAGCAG AAGTGTCTAA ACCAAGCACT CAGAAAAAAG AGGAAAAACT GGCAGCAGCA 
GCAGACACAT CCAAACCAAG CACTCAGAAA AAAGAGGTAA AAGCCACAGC AAAAGCCACA 
GCTGCCGAAG CTTCCAAACA AAGCACTGAG TCCATACCAT CAGAAGCAAT AGACGAGTTG 
TTGGGAACAC TTGAAGGACC CCCAGAAAAT GTTCCAGGGT CTCCAGTATT TCAGGGTCCA 
GAAGTTACTG AGAACATTAC AGCAACATAC TTAGAAGAAA CTGGAAAAAG AGATAGTACG 
ATCCCTCCAG AATACAGAAA ATTATTAGAT GGAAAGGATC ATGGGAAAGA TGTGTGGCCT 
TCAGCACCTG AACCAGAACC GTCTATGACC GACACAGAGC TTGTAGATGT GTTTTCCAAA 
GACTTTGAGC TTTCATGTTC ACCTACTGTT CAGACCACGC CAGCCATTAA ACCCAAAAGG 
AACACTGAAG ATCTCCACGA GTGCTATTGC TCAAAGTCAG TGAGCCAACT TTCATCATCT 
GAAACTCCTC AAGGTAACAA AACGAAACCT GAAATTGCGG CATCATCTGC ATCCACTGTA 
AAAGCAGCAG CGCCCTCCGG TGGTATGGAC AGTGCATTAG ATGAATTATT GGGAACACTG 
GAAGGTCCTG ATCCAACAGC CCCTGAATCA CCTGTGTACA CTGGGCCAGA GGTGACGGAA 
ACAGAGACAG CGGTATACAG AGAGGGACTT GGGATAAGAG ACAGCAGCAT CCCTCCAGCA 
TACAGGCACT TACTAGATGG GAAAGTAGAT GGAAAAGATG CACCCCCACC ACCGCCAGAG 
GAAAAGCCTC TGACCGATGA TCAGCTGATT GATAAGTTTT CTATGGACTT TGATTGTTCT 
GAATCTCCTG CAACACAACA GGCTCATCCT GTCGAACCTA AAGTTTCCTC GCAAGAGAAA 
AAGGCAGAAA AAGAAGTAGT TGCTGCCTCC TCTGCTACTG TTAAAGCTGC ACCCACTCCT 
TCTAAAGCAC CAGCACCTCC AGCTGATCCA CTCGATAGCC TGTCTGGTAC CTTAGGTGTT 
CGAAAGGAAG AACCGAGTGA CAAGAAGCCT CCAGTGGATA AAGTAAAGGA GGGAACAGGC 
AAAGAGAAGA AAGAAAAGCT TGGAGAAGAT GAAAAAACAA TACATCCTGA CCGCCGGCTA 
AAGGAAGTGA AGGATAAAGA CGGAAAGCCC TTGCTGCCAA AACTTGAACA AAGCCCTAAG 
CCAATGAGTG AGGCCGAGCT GCTGGATGCT TTGACTGAAG GCTTTGTGAC TTCCCCTGCC 
ACTCCTGATG CCCCACTGCA GGGCTCTTTG AAAAAAAAAG GTTCTGGATC AGAAGATGTT 
ATATCTTGTT CTAAAGCTTC CGCCGTCAAG TCTGGAGCTC CACAGGACGC TGCTTTAGAT 
ATACAGATTC CCGACGATGC ACTAGATTTA CTTTCTGGCT CCCTGGGACA AAGAGAGGTG 
GATCCTAATG AAAACAAACC GGTGGTGGAT GTAGTTAAGG AAAAAGCTAA AGCTGAGCAT 
ATAGACAAAC TGGGGGACAG AGATGATACA ATCCCACCGG AATACAGAAA GCTGCTGGAT 
GGTAAAGATG ATAAGGGACA ACCTGCAAAA CCACCAGCGA AAGCGGAGGA GAAACCAAAG 
AAGCCTTTAA GTGACGATGC AGCGATCGAT GCTCTTTCAA GCGGATTTGC ATCATGTGAC 
ACAAAATCTA CTGAACAACC AAAGCAGCCT TCAACGGACA AGACTGAGAA ACAATGTGCT 
AGCGCTCCAG TGTCGCAGCC TTCAGGAAAG GCACCTGATG CTGCAAAGCA GGCAAACTCA 
GCGATACCAA GTGTGCCAAA GGCTGGCAAA AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	cast
Entrez Gene ID	100145371
Full Name	calpastatin
Synonyms	calp3,xcalp3
General protein information	Preferred Names calpastatin Names calpastatin
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	CAST	NP_001035905.1
	CAST	XP_001091928.2
Mus musculus (house mouse)	Cast	NP_033947.1
Rattus norvegicus (Norway rat)	Cast	NP_445747.2
Gallus gallus (chicken)	CAST	NP_001131122.1
Pan troglodytes (chimpanzee)	CAST	XP_518168.4
Canis lupus familiaris (dog)	CAST	XP_005618134.1
Bos taurus (cattle)	CAST	NP_001025489.1
Xenopus tropicalis (tropical clawed frog)	cast	NP_001120309.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

cast cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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