f5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	f5
Entrez Gene ID	100135376
Full Name	coagulation factor V (proaccelerin, labile factor)
General protein information	Preferred Names coagulation factor V (proaccelerin, labile factor) Names coagulation factor V
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935774.5	XP_002935820.2	coagulation factor V
XM_002935774.4	XP_002935820.2	coagulation factor V

Pathways from BioSystems

Homologies

Bos taurus (cattle)	F5	NP_776304.1
Rattus norvegicus (Norway rat)	F5	NP_001041343.1
Gallus gallus (chicken)	F5	XP_001231901.3
Danio rerio (zebrafish)	f5	NP_001007209.2
Mus musculus (house mouse)	F5	NP_032002.1
Pan troglodytes (chimpanzee)	F5	XP_513984.4
Homo sapiens (human)	F5	NP_000121.2
	F5	XP_001093072.2
Canis lupus familiaris (dog)	F5	XP_005622605.1
Xenopus tropicalis (tropical clawed frog)	f5	XP_002935820.2

Gene Ontology

The following f5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the f5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05814	XM_002935774.5 Latest version!	Xenopus tropicalis coagulation factor V (proaccelerin, labile factor) (f5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OXa05814	XM_002935774.4	Xenopus tropicalis coagulation factor V (proaccelerin, labile factor) (f5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

f5 ( XM_002935774.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935820.2 Xenopus tropicalis coagulation factor V (proaccelerin, labile factor) (f5), mRNA.

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CloneID	OXa05814
Clone ID Related Accession (Same CDS sequence)	XM_002935774.5 , XM_002935774.4
Accession Version	XM_002935774.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coagulation factor V
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935774.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGGA AGAGAATTCT CTCCTCTTCT TTGTTCATTT TGTTCCTTCT GGGCACTGTA 
TTGCAGTCTG CTCCCAAAGT CTCAGCGGCT GTGAGGGAAT ATTATATTGC TGCTGTGATC 
ACAGACTGGA GCTACACCAA TCAATATCAT GACAGTTCAG AATTTCTATA CAAGAAGATT 
ATTTATAGAG AATATGAAGA AGGATTTCAA AAGGCAAAAC CAGCTGTTGA GTATTCAGGT 
ATTCTGGGGC CTACATTACG TGCAGAAGTT GGAGACATAC TACAAGTTCA TTTTAAAAAT 
ATGGCTAATA AACCGCTGAC TATTCATCCT CAAGGTGTTG CTTATGGGAA GAAATCAGAA 
GGGGCCAAAT ATACAGACAG TACTTTACCT TATGAAAAGC TGGATGACTC TGTTATGCCA 
GGACAAATGT ACACTTACAT CTGGGAAATT ACTGAAGAAA TAGGACCCAA GGAATATGAC 
CCAGCTTGTC TCACAAATAT CTATTACTCC CATGAAAATA TGGTTCAAGA TTTTAACTCT 
GGACTGATTG GTGCTTTGCT TATCTGTAAA AAAGGAAGTT TAAATGAAGT TGGGGAGCAA 
CGCCATTTCG ATAAAGAGTA TGTGCTGATG TTTGCTGTAT TTGATGAAAA CAAAAGCTGG 
CAAAATTTGA ACGATAAAGA ACACTCTGTG ATGTATTCTA TAAATGGATA CGTTAATGGC 
ACAATTCCAG ATATCTATGC CTGTGCTGGG GACTCGGTTA GCTGGCATTT GTTAGGTATG 
AGCTCCGAAC CAGAATTTTT CTCTATTCAT TTCTTTGGTC AAACATTAGA AGAAAACCAA 
CATAAGGTTT CAGTAGTTGG CCTTGTTGCT TCTTCATCCA CCACAGCCAA CATGACAATA 
TACCAAACTG GGAAATGGTT AATAACATCC CTTGTTGAAA AGCATTTTGA GGCTGGGATG 
CATGGATACA TTGAAGTGCA GCCCTGCAGT GATAAGGGAT ACACAGCACC AAAATTATCA 
CTTCTTCAAA AGCGATTCAT AAAAGTTTGG GAGTACTTCA TAGCAGCAGA GGAAGAGGAG 
TGGAACTATG TCCCTTCAAA GGTAGAGAAC ACAGACAAGA TAAACTGCCA ACAAAAGTAT 
AAGAAAGTTC TCTACAAGGC ATATTCTGAT AACACATTCA CAAAGCTCTT GACAGCAGGG 
AATGAGGGCC TTCTTGGGCC TGTCATTAGA GCTCAGGTCA GAGATACAAT TACAGTTGTT 
TTCAAAAATA AGGCCAAACG GCCTTACAGC ATATACCCCC ATGGTGTGTC AGTACAAAAA 
GCCTATGAAG GTGCCAGTTA CCCCCCTGAT ATAAGAGGGA ATGAAACACA AAATCAAGCA 
GTAATGCCAG GAGAAACAGT GACGTACTAC TGGAATATTC TAGAAACTGA TGAACCTGCA 
GCACGGGATC CCCGTTGTTT AACAAGGATG TACCATAGTG CTGTTAATAT TACAAAAGAT 
ATTGCCTCTG GCTTAATCGG TCCGCTCCTC ATTTGCAAAT CAATGTCTCT AAATACAAGA 
GGTGTGCAGG AAAAGGCTGA TTTGGAACAA ATTGCAACAT TTGCTCTGTT TGATGAAAAC 
AAAAGTTGGT ACCATAATGA AAACAAGCTT AAGCATTGTC ATGCCGTTAA CAACGATCAA 
ACAGAGTACT ACAGCCATAA TATTATACCC ACCATAAATG GATATGCGTT TGAATCTGCG 
CCTTTGGGAT TCTGTCATAG CCAGGTGATC CACTGGCATA TTTCCAGCGT GGGTGCCCAA 
GATGAAATAA TTGGAGTACA TCTTTCAGGT CATACCCTCA GATACAAGGG AAAAAGTGAA 
GATACAGTAA ATATATTCCC GATGAGTGGT GAAAGTATTT CTGTAGATAT GGATAATCTT 
GGTTTATGGC TGTTTGGGGT GTTTGGGTCT TCTAAGAAAA ACCAGGGACT GAAAGTGAGA 
TTCACTGATG TCATCTGTGT AGAGGAAGAA GAAGATTATG AGGAACCAAA TATACAGTTT 
GGGATAAGTA GTGAAGAAAT TACAAAAAAA ATTGTTACAT CCCCTGAAAC CGACTACAGT 
GAGGAAGAAG ATGTAAACAC TAATTTAGAT ATAGACTACA CAGATAATTT AGCTGATTTG 
TTCCACATTC GGTCATTCAG AAACAAGACA GAAACACAGG AAGAAGGTGT AAATCTTACT 
GCTTTGGCCA TAGAGCACAC CTATGGGAGA CAAAATGAAA GCATGGGTGA CTTTAGTAAA 
CTTGAAGTTT CGGAGGAATA TGACATTGTT CCCATTGAGG ATAATGAAAA CTCAAATTTC 
AATCCTACGT CTGCAAGTAA AGCAGATAAA CTAACTAAAG ATGGCTTACC CAAAACACCT 
CAATACATGC AATCTAAAGA TGAAGATACC TTGGAGAATA CCTTGTCTGA GGAGTCTAAT 
ATGGCACAGA AAAATGTCAC AGTATATAAT CCTTTAGACA GTGAAAAAGA ACAATTTATC 
ATAATGGAGG GAAATCTTAC TACTGACAAA AACAGAGATC TATCATATGA CAGTTACTTT 
AATGAAGCAG ACAAAAATAT TTATAGTGAG GAAGACAAAG CTAAAATGAT CACTGAACAA 
AACACCTCAA ATTTGTTGGC GGAATCCAAA CCACAATTGA GCAATACAGA AAAAAGCCAT 
TTCATAAAAG AAGACCTATC ACACAGCATA AATAAAACAT TTTATTATTT GGTCACAACA 
TATGATAATG ACACACAAAA TTCAGAAACC CAGCATAATG AGTTTATGGC TTTGAAAGTT 
GCAGAAAAGA TTATTGATAC TCACCAAAAA GTTCCCAGGC AGGAGGAAAA AATGACAGAA 
ATTAGTGATC ATGTGACGAC TGTAGAGAAT AATACGTTAG ATATAACAAA GCATGATTTT 
CCTCTTAGAG AAGATCTTTT CAATGCCACT GATTCAAAGG AATACAATCC TCAGGTGGCA 
GACATAAAGG AAAAAAAATT GGTAAAAAGA TCCATTGAAC ACATGACAGA GAGTCCCACC 
TACTCTCCCT TAAATGTGGA TCATTTTCTG GAAACAGAAA ATGAAAAGTC ACATTCTTCC 
GATAATAAAG ACATCATGGA TGACTTCAGT ACAAATCAGG GACTTGACAG CAATGATACT 
TTTAATGATT TCATCAAAAA TCAGACAAGT GAAAGCAATT ACAATACCGA TAACATCAGC 
AAAAATCAGA CAGTTGCAAG CAGTGACATA ACTGACGACT TCAGCAAAAA TGGAACAACT 
AAAAACCTAC TATCATCAAC AAATATAACC AGTGGTATTA AAGAGCTACA TTCTTCAAAA 
AATAATGACC TTACTGATTA TTTAGATCAC GACAAAACAC CTGAAAAATT AATCCCATAC 
TTTAATGACA GCAATTTAAA TCTGTTGCCT AACAGGACAT CGCATAGCCT GGAGGAGAGT 
AAGCTACCCA ATGGTAACAG TAACAATTAT GGAAATCAAA CTGCAGCAAC AAGGGATATT 
GACTCTCCTG AAACTAAATC GGACACTGAA TTTAGAGAAT TGTTTGGACA ACAGATTTTT 
GAAGGTATAA TGCAAGTCAT ATTCTATCAG AAGGAAGTAA ATGAAACAAA CATGGAGCAG 
CATCTTGTTA CAGAAGAAAA CTCTACTGAA AGTGTGCAAA ATGAGTCACA TACACTTTTT 
AATAACAAAA CACAAGAAGA ACAAAATGCA AAAAAACCCA CAAAGCGATA TTTTCAAGTG 
AGACCAATCA GGTACAGAAC TTCAAGTAAC GAAACTAGAA TGTTAACTCG AAGAAAAAAA 
AGACTGGGAA ACGTCAATGG ATCCCTAAGC AATGACACTG GTAATGATGG AGTGTTCTCT 
CCCAGAGGAA TGAGACCACA AATTATAATT GGACTTCCTG GACTTGATGA GGGTGATTAT 
GTTGAGCTTA ATGTAGATGA AATCGATGAA GATGTACATA TTAAAAAAGT TGAATATGAG 
GAACTTTACA AAACAGAAGC GCAAGAGTAC ACTAACCCAG ATAAGATAAT ACAGCAATAC 
CTGCGTAGTT TAAAAGGAAA AAAAAGAAAG TATTACATCA CTGCAGAAGA GGAACAATGG 
GATTACTACA ATCCTGCAAA AAGTTCTTTA ACAAAAGAAA GGCCAGACAG TGAAACAAGG 
ACTACCCAGT ACACAAAAGT ACGGTTTCGG CAATATCTTG ATTCCAGTTT TAGCAAGCCT 
GATGTTCAAG GGGAATATGA AGAACACTTA GGTATCCTTG GTCCTGTAAT CCGAGCTGAA 
GTTGATGATA TTATTCAGAT AACGTTTAAA AACTTAGCAT CACGTCCCTA TTCTCTTCAT 
GCACACGGGG TTTCTTATGA AAAATCATCT GAAGGATTTC AGTATGATGA TGGGACCCCA 
GACTGGCTTA AGAAAGATGA TGCGGTTAAA CCAGGTGAAA CCTATACCTA TGTTTGGTAT 
GCCACACACC AATCAGCACC TGAAAATGAA GGCTCAGATT GCAGAACATG GATCTATTAT 
TCTGGAGTTA ACCCGGAAAA AGATATACAT TCAGGACTTC TGGGCCCACT GCTGATCTGT 
AAAAGCGGAA CACTTCATAA ACATAGCAAC AGGCCATTGG ATGTACGAGA ATTTGTATTG 
CTATTTATGA CATTTGAAGA AGAGAAAAGC TGGTACTTTG AAAAAAACAA GAAAAAGACC 
TGCTTGGAAG TCATGGAAAA ACCTTTACAA GCAGCAAAGT GTCATACATT TCACGCTATA 
AATGGCAAAA TCTACAACCT GAAAGGCTTA GTGATGTATC AGAATGAATC AGTGCGATGG 
CATATGATAA ATATGGGAGG TTCAAAGGAT TTACATGTGG TTCATTTCCA TGGTCAGACT 
GTGACAGAGA GGATTAACAG AGAGTCTCAA CGCGGTGTCT ACCCTCTTCT ACCTGGTTCA 
TTTGCAACAG TGGAAATGAA GCCATCGAAG CCTGGACATT GGCTCTTGGA TACAGAAGTT 
GCTGAGTACC AACAAGCAGG AATGCAGGCA ATGTTTCACA TTGTTGAGGC TGAATGCAAT 
TTACCAATGG GACTTTCAAA TGGAATTATA TCTGACAATC AAATCACTGC ATCACACTAT 
ATAGATTATT GGGAGCCCAG ACTGGCTAGG TTAAACAATG CAGGATCTTA CAATGCTTGG 
AGCACTGTAA TGAATATGTC AGCACTACCA TGGATTCAGG TGGATTTGCA AAGGCCTTTT 
TTAATAACAG GTATTAAAAC ACAGGGAGCA AGTAAATATC TTAAGCCATA TTACGTCAAA 
GAATTCTTTG TTGTGTACAG TTTGGATAAG AAGAATTGGA TAGCTTTTAA AGGAAACAGT 
ATTACACTGC AAATGTTCTT TGATGGTAAT ACCGATGCAC ACAGTATTAA GGAAAACACA 
TTGGACCCAC CAATTTCTGC CCAGTACATA AGAGTATATC CAACCAAATA CTACAACAGA 
CCAACTCTTC GTATGGAGCT ATTTGGGTGT GAAATACTAG GATGCTTTAT GCCGCTGGGC 
ATGGAAAACG GTATTATAAA GGATGAGCAG ATCACAGCTT CTTCTTATAA GAGTTCATGG 
TATTCCTCAT GGGTGCCATC TTTGGCTCGT CTTAATAAAG TAGGCAAATC CAATGCCTGG 
CAAGCAAAGT CAAATAACAA CCAACAGTGG CTACAAATTG ACTTTTTGAT GACAAAGAGG 
ATAACTGGTA TAACAACACA GGGGGCCAAG GCATTGACAA CTGATATGTA TGTAAAATCT 
TACAGTATCC AGTACAGTGA CAATGGGAAA GACTGGAAAC CATACACTGA TGAATTAGCA 
CCATTAGAAA AGGTATTCCT AGGAAATGCA AACAGCTATG GACTTGTCAA GAATGAAATA 
AGATCACCAA TATTGGCACG TTTCTTAAGG ATTATCCCTA AGAGCTGGCA CAACAGCATC 
ACACTGAGAA TAGAAATCTT TGGTTGTAAT ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935820.2
CDS	229..6264
Translation	MGRKRILSSSLFILFLLGTVLQSAPKVSAAVREYYIAAVITDWSYTNQYHDSSEFLYKKIIYREYEEGFQKAKPAVEYSGILGPTLRAEVGDILQVHFKNMANKPLTIHPQGVAYGKKSEGAKYTDSTLPYEKLDDSVMPGQMYTYIWEITEEIGPKEYDPACLTNIYYSHENMVQDFNSGLIGALLICKKGSLNEVGEQRHFDKEYVLMFAVFDENKSWQNLNDKEHSVMYSINGYVNGTIPDIYACAGDSVSWHLLGMSSEPEFFSIHFFGQTLEENQHKVSVVGLVASSSTTANMTIYQTGKWLITSLVEKHFEAGMHGYIEVQPCSDKGYTAPKLSLLQKRFIKVWEYFIAAEEEEWNYVPSKVENTDKINCQQKYKKVLYKAYSDNTFTKLLTAGNEGLLGPVIRAQVRDTITVVFKNKAKRPYSIYPHGVSVQKAYEGASYPPDIRGNETQNQAVMPGETVTYYWNILETDEPAARDPRCLTRMYHSAVNITKDIASGLIGPLLICKSMSLNTRGVQEKADLEQIATFALFDENKSWYHNENKLKHCHAVNNDQTEYYSHNIIPTINGYAFESAPLGFCHSQVIHWHISSVGAQDEIIGVHLSGHTLRYKGKSEDTVNIFPMSGESISVDMDNLGLWLFGVFGSSKKNQGLKVRFTDVICVEEEEDYEEPNIQFGISSEEITKKIVTSPETDYSEEEDVNTNLDIDYTDNLADLFHIRSFRNKTETQEEGVNLTALAIEHTYGRQNESMGDFSKLEVSEEYDIVPIEDNENSNFNPTSASKADKLTKDGLPKTPQYMQSKDEDTLENTLSEESNMAQKNVTVYNPLDSEKEQFIIMEGNLTTDKNRDLSYDSYFNEADKNIYSEEDKAKMITEQNTSNLLAESKPQLSNTEKSHFIKEDLSHSINKTFYYLVTTYDNDTQNSETQHNEFMALKVAEKIIDTHQKVPRQEEKMTEISDHVTTVENNTLDITKHDFPLREDLFNATDSKEYNPQVADIKEKKLVKRSIEHMTESPTYSPLNVDHFLETENEKSHSSDNKDIMDDFSTNQGLDSNDTFNDFIKNQTSESNYNTDNISKNQTVASSDITDDFSKNGTTKNLLSSTNITSGIKELHSSKNNDLTDYLDHDKTPEKLIPYFNDSNLNLLPNRTSHSLEESKLPNGNSNNYGNQTAATRDIDSPETKSDTEFRELFGQQIFEGIMQVIFYQKEVNETNMEQHLVTEENSTESVQNESHTLFNNKTQEEQNAKKPTKRYFQVRPIRYRTSSNETRMLTRRKKRLGNVNGSLSNDTGNDGVFSPRGMRPQIIIGLPGLDEGDYVELNVDEIDEDVHIKKVEYEELYKTEAQEYTNPDKIIQQYLRSLKGKKRKYYITAEEEQWDYYNPAKSSLTKERPDSETRTTQYTKVRFRQYLDSSFSKPDVQGEYEEHLGILGPVIRAEVDDIIQITFKNLASRPYSLHAHGVSYEKSSEGFQYDDGTPDWLKKDDAVKPGETYTYVWYATHQSAPENEGSDCRTWIYYSGVNPEKDIHSGLLGPLLICKSGTLHKHSNRPLDVREFVLLFMTFEEEKSWYFEKNKKKTCLEVMEKPLQAAKCHTFHAINGKIYNLKGLVMYQNESVRWHMINMGGSKDLHVVHFHGQTVTERINRESQRGVYPLLPGSFATVEMKPSKPGHWLLDTEVAEYQQAGMQAMFHIVEAECNLPMGLSNGIISDNQITASHYIDYWEPRLARLNNAGSYNAWSTVMNMSALPWIQVDLQRPFLITGIKTQGASKYLKPYYVKEFFVVYSLDKKNWIAFKGNSITLQMFFDGNTDAHSIKENTLDPPISAQYIRVYPTKYYNRPTLRMELFGCEILGCFMPLGMENGIIKDEQITASSYKSSWYSSWVPSLARLNKVGKSNAWQAKSNNNQQWLQIDFLMTKRITGITTQGAKALTTDMYVKSYSIQYSDNGKDWKPYTDELAPLEKVFLGNANSYGLVKNEIRSPILARFLRIIPKSWHNSITLRIEIFGCNI

Target ORF information:

RefSeq Version	XM_002935774.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coagulation factor V (proaccelerin, labile factor) (f5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935774.5

ORF Insert Sequence:

ATGGGCCGGA AGAGAATTCT CTCCTCTTCT TTGTTCATTT TGTTCCTTCT GGGCACTGTA 
TTGCAGTCTG CTCCCAAAGT CTCAGCGGCT GTGAGGGAAT ATTATATTGC TGCTGTGATC 
ACAGACTGGA GCTACACCAA TCAATATCAT GACAGTTCAG AATTTCTATA CAAGAAGATT 
ATTTATAGAG AATATGAAGA AGGATTTCAA AAGGCAAAAC CAGCTGTTGA GTATTCAGGT 
ATTCTGGGGC CTACATTACG TGCAGAAGTT GGAGACATAC TACAAGTTCA TTTTAAAAAT 
ATGGCTAATA AACCGCTGAC TATTCATCCT CAAGGTGTTG CTTATGGGAA GAAATCAGAA 
GGGGCCAAAT ATACAGACAG TACTTTACCT TATGAAAAGC TGGATGACTC TGTTATGCCA 
GGACAAATGT ACACTTACAT CTGGGAAATT ACTGAAGAAA TAGGACCCAA GGAATATGAC 
CCAGCTTGTC TCACAAATAT CTATTACTCC CATGAAAATA TGGTTCAAGA TTTTAACTCT 
GGACTGATTG GTGCTTTGCT TATCTGTAAA AAAGGAAGTT TAAATGAAGT TGGGGAGCAA 
CGCCATTTCG ATAAAGAGTA TGTGCTGATG TTTGCTGTAT TTGATGAAAA CAAAAGCTGG 
CAAAATTTGA ACGATAAAGA ACACTCTGTG ATGTATTCTA TAAATGGATA CGTTAATGGC 
ACAATTCCAG ATATCTATGC CTGTGCTGGG GACTCGGTTA GCTGGCATTT GTTAGGTATG 
AGCTCCGAAC CAGAATTTTT CTCTATTCAT TTCTTTGGTC AAACATTAGA AGAAAACCAA 
CATAAGGTTT CAGTAGTTGG CCTTGTTGCT TCTTCATCCA CCACAGCCAA CATGACAATA 
TACCAAACTG GGAAATGGTT AATAACATCC CTTGTTGAAA AGCATTTTGA GGCTGGGATG 
CATGGATACA TTGAAGTGCA GCCCTGCAGT GATAAGGGAT ACACAGCACC AAAATTATCA 
CTTCTTCAAA AGCGATTCAT AAAAGTTTGG GAGTACTTCA TAGCAGCAGA GGAAGAGGAG 
TGGAACTATG TCCCTTCAAA GGTAGAGAAC ACAGACAAGA TAAACTGCCA ACAAAAGTAT 
AAGAAAGTTC TCTACAAGGC ATATTCTGAT AACACATTCA CAAAGCTCTT GACAGCAGGG 
AATGAGGGCC TTCTTGGGCC TGTCATTAGA GCTCAGGTCA GAGATACAAT TACAGTTGTT 
TTCAAAAATA AGGCCAAACG GCCTTACAGC ATATACCCCC ATGGTGTGTC AGTACAAAAA 
GCCTATGAAG GTGCCAGTTA CCCCCCTGAT ATAAGAGGGA ATGAAACACA AAATCAAGCA 
GTAATGCCAG GAGAAACAGT GACGTACTAC TGGAATATTC TAGAAACTGA TGAACCTGCA 
GCACGGGATC CCCGTTGTTT AACAAGGATG TACCATAGTG CTGTTAATAT TACAAAAGAT 
ATTGCCTCTG GCTTAATCGG TCCGCTCCTC ATTTGCAAAT CAATGTCTCT AAATACAAGA 
GGTGTGCAGG AAAAGGCTGA TTTGGAACAA ATTGCAACAT TTGCTCTGTT TGATGAAAAC 
AAAAGTTGGT ACCATAATGA AAACAAGCTT AAGCATTGTC ATGCCGTTAA CAACGATCAA 
ACAGAGTACT ACAGCCATAA TATTATACCC ACCATAAATG GATATGCGTT TGAATCTGCG 
CCTTTGGGAT TCTGTCATAG CCAGGTGATC CACTGGCATA TTTCCAGCGT GGGTGCCCAA 
GATGAAATAA TTGGAGTACA TCTTTCAGGT CATACCCTCA GATACAAGGG AAAAAGTGAA 
GATACAGTAA ATATATTCCC GATGAGTGGT GAAAGTATTT CTGTAGATAT GGATAATCTT 
GGTTTATGGC TGTTTGGGGT GTTTGGGTCT TCTAAGAAAA ACCAGGGACT GAAAGTGAGA 
TTCACTGATG TCATCTGTGT AGAGGAAGAA GAAGATTATG AGGAACCAAA TATACAGTTT 
GGGATAAGTA GTGAAGAAAT TACAAAAAAA ATTGTTACAT CCCCTGAAAC CGACTACAGT 
GAGGAAGAAG ATGTAAACAC TAATTTAGAT ATAGACTACA CAGATAATTT AGCTGATTTG 
TTCCACATTC GGTCATTCAG AAACAAGACA GAAACACAGG AAGAAGGTGT AAATCTTACT 
GCTTTGGCCA TAGAGCACAC CTATGGGAGA CAAAATGAAA GCATGGGTGA CTTTAGTAAA 
CTTGAAGTTT CGGAGGAATA TGACATTGTT CCCATTGAGG ATAATGAAAA CTCAAATTTC 
AATCCTACGT CTGCAAGTAA AGCAGATAAA CTAACTAAAG ATGGCTTACC CAAAACACCT 
CAATACATGC AATCTAAAGA TGAAGATACC TTGGAGAATA CCTTGTCTGA GGAGTCTAAT 
ATGGCACAGA AAAATGTCAC AGTATATAAT CCTTTAGACA GTGAAAAAGA ACAATTTATC 
ATAATGGAGG GAAATCTTAC TACTGACAAA AACAGAGATC TATCATATGA CAGTTACTTT 
AATGAAGCAG ACAAAAATAT TTATAGTGAG GAAGACAAAG CTAAAATGAT CACTGAACAA 
AACACCTCAA ATTTGTTGGC GGAATCCAAA CCACAATTGA GCAATACAGA AAAAAGCCAT 
TTCATAAAAG AAGACCTATC ACACAGCATA AATAAAACAT TTTATTATTT GGTCACAACA 
TATGATAATG ACACACAAAA TTCAGAAACC CAGCATAATG AGTTTATGGC TTTGAAAGTT 
GCAGAAAAGA TTATTGATAC TCACCAAAAA GTTCCCAGGC AGGAGGAAAA AATGACAGAA 
ATTAGTGATC ATGTGACGAC TGTAGAGAAT AATACGTTAG ATATAACAAA GCATGATTTT 
CCTCTTAGAG AAGATCTTTT CAATGCCACT GATTCAAAGG AATACAATCC TCAGGTGGCA 
GACATAAAGG AAAAAAAATT GGTAAAAAGA TCCATTGAAC ACATGACAGA GAGTCCCACC 
TACTCTCCCT TAAATGTGGA TCATTTTCTG GAAACAGAAA ATGAAAAGTC ACATTCTTCC 
GATAATAAAG ACATCATGGA TGACTTCAGT ACAAATCAGG GACTTGACAG CAATGATACT 
TTTAATGATT TCATCAAAAA TCAGACAAGT GAAAGCAATT ACAATACCGA TAACATCAGC 
AAAAATCAGA CAGTTGCAAG CAGTGACATA ACTGACGACT TCAGCAAAAA TGGAACAACT 
AAAAACCTAC TATCATCAAC AAATATAACC AGTGGTATTA AAGAGCTACA TTCTTCAAAA 
AATAATGACC TTACTGATTA TTTAGATCAC GACAAAACAC CTGAAAAATT AATCCCATAC 
TTTAATGACA GCAATTTAAA TCTGTTGCCT AACAGGACAT CGCATAGCCT GGAGGAGAGT 
AAGCTACCCA ATGGTAACAG TAACAATTAT GGAAATCAAA CTGCAGCAAC AAGGGATATT 
GACTCTCCTG AAACTAAATC GGACACTGAA TTTAGAGAAT TGTTTGGACA ACAGATTTTT 
GAAGGTATAA TGCAAGTCAT ATTCTATCAG AAGGAAGTAA ATGAAACAAA CATGGAGCAG 
CATCTTGTTA CAGAAGAAAA CTCTACTGAA AGTGTGCAAA ATGAGTCACA TACACTTTTT 
AATAACAAAA CACAAGAAGA ACAAAATGCA AAAAAACCCA CAAAGCGATA TTTTCAAGTG 
AGACCAATCA GGTACAGAAC TTCAAGTAAC GAAACTAGAA TGTTAACTCG AAGAAAAAAA 
AGACTGGGAA ACGTCAATGG ATCCCTAAGC AATGACACTG GTAATGATGG AGTGTTCTCT 
CCCAGAGGAA TGAGACCACA AATTATAATT GGACTTCCTG GACTTGATGA GGGTGATTAT 
GTTGAGCTTA ATGTAGATGA AATCGATGAA GATGTACATA TTAAAAAAGT TGAATATGAG 
GAACTTTACA AAACAGAAGC GCAAGAGTAC ACTAACCCAG ATAAGATAAT ACAGCAATAC 
CTGCGTAGTT TAAAAGGAAA AAAAAGAAAG TATTACATCA CTGCAGAAGA GGAACAATGG 
GATTACTACA ATCCTGCAAA AAGTTCTTTA ACAAAAGAAA GGCCAGACAG TGAAACAAGG 
ACTACCCAGT ACACAAAAGT ACGGTTTCGG CAATATCTTG ATTCCAGTTT TAGCAAGCCT 
GATGTTCAAG GGGAATATGA AGAACACTTA GGTATCCTTG GTCCTGTAAT CCGAGCTGAA 
GTTGATGATA TTATTCAGAT AACGTTTAAA AACTTAGCAT CACGTCCCTA TTCTCTTCAT 
GCACACGGGG TTTCTTATGA AAAATCATCT GAAGGATTTC AGTATGATGA TGGGACCCCA 
GACTGGCTTA AGAAAGATGA TGCGGTTAAA CCAGGTGAAA CCTATACCTA TGTTTGGTAT 
GCCACACACC AATCAGCACC TGAAAATGAA GGCTCAGATT GCAGAACATG GATCTATTAT 
TCTGGAGTTA ACCCGGAAAA AGATATACAT TCAGGACTTC TGGGCCCACT GCTGATCTGT 
AAAAGCGGAA CACTTCATAA ACATAGCAAC AGGCCATTGG ATGTACGAGA ATTTGTATTG 
CTATTTATGA CATTTGAAGA AGAGAAAAGC TGGTACTTTG AAAAAAACAA GAAAAAGACC 
TGCTTGGAAG TCATGGAAAA ACCTTTACAA GCAGCAAAGT GTCATACATT TCACGCTATA 
AATGGCAAAA TCTACAACCT GAAAGGCTTA GTGATGTATC AGAATGAATC AGTGCGATGG 
CATATGATAA ATATGGGAGG TTCAAAGGAT TTACATGTGG TTCATTTCCA TGGTCAGACT 
GTGACAGAGA GGATTAACAG AGAGTCTCAA CGCGGTGTCT ACCCTCTTCT ACCTGGTTCA 
TTTGCAACAG TGGAAATGAA GCCATCGAAG CCTGGACATT GGCTCTTGGA TACAGAAGTT 
GCTGAGTACC AACAAGCAGG AATGCAGGCA ATGTTTCACA TTGTTGAGGC TGAATGCAAT 
TTACCAATGG GACTTTCAAA TGGAATTATA TCTGACAATC AAATCACTGC ATCACACTAT 
ATAGATTATT GGGAGCCCAG ACTGGCTAGG TTAAACAATG CAGGATCTTA CAATGCTTGG 
AGCACTGTAA TGAATATGTC AGCACTACCA TGGATTCAGG TGGATTTGCA AAGGCCTTTT 
TTAATAACAG GTATTAAAAC ACAGGGAGCA AGTAAATATC TTAAGCCATA TTACGTCAAA 
GAATTCTTTG TTGTGTACAG TTTGGATAAG AAGAATTGGA TAGCTTTTAA AGGAAACAGT 
ATTACACTGC AAATGTTCTT TGATGGTAAT ACCGATGCAC ACAGTATTAA GGAAAACACA 
TTGGACCCAC CAATTTCTGC CCAGTACATA AGAGTATATC CAACCAAATA CTACAACAGA 
CCAACTCTTC GTATGGAGCT ATTTGGGTGT GAAATACTAG GATGCTTTAT GCCGCTGGGC 
ATGGAAAACG GTATTATAAA GGATGAGCAG ATCACAGCTT CTTCTTATAA GAGTTCATGG 
TATTCCTCAT GGGTGCCATC TTTGGCTCGT CTTAATAAAG TAGGCAAATC CAATGCCTGG 
CAAGCAAAGT CAAATAACAA CCAACAGTGG CTACAAATTG ACTTTTTGAT GACAAAGAGG 
ATAACTGGTA TAACAACACA GGGGGCCAAG GCATTGACAA CTGATATGTA TGTAAAATCT 
TACAGTATCC AGTACAGTGA CAATGGGAAA GACTGGAAAC CATACACTGA TGAATTAGCA 
CCATTAGAAA AGGTATTCCT AGGAAATGCA AACAGCTATG GACTTGTCAA GAATGAAATA 
AGATCACCAA TATTGGCACG TTTCTTAAGG ATTATCCCTA AGAGCTGGCA CAACAGCATC 
ACACTGAGAA TAGAAATCTT TGGTTGTAAT ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

f5 ( XM_002935774.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935820.2 Xenopus tropicalis coagulation factor V (proaccelerin, labile factor) (f5), mRNA.

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CloneID	OXa05814
Clone ID Related Accession (Same CDS sequence)	XM_002935774.5 , XM_002935774.4
Accession Version	XM_002935774.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	coagulation factor V
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935774.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCCGGA AGAGAATTCT CTCCTCTTCT TTGTTCATTT TGTTCCTTCT GGGCACTGTA 
TTGCAGTCTG CTCCCAAAGT CTCAGCGGCT GTGAGGGAAT ATTATATTGC TGCTGTGATC 
ACAGACTGGA GCTACACCAA TCAATATCAT GACAGTTCAG AATTTCTATA CAAGAAGATT 
ATTTATAGAG AATATGAAGA AGGATTTCAA AAGGCAAAAC CAGCTGTTGA GTATTCAGGT 
ATTCTGGGGC CTACATTACG TGCAGAAGTT GGAGACATAC TACAAGTTCA TTTTAAAAAT 
ATGGCTAATA AACCGCTGAC TATTCATCCT CAAGGTGTTG CTTATGGGAA GAAATCAGAA 
GGGGCCAAAT ATACAGACAG TACTTTACCT TATGAAAAGC TGGATGACTC TGTTATGCCA 
GGACAAATGT ACACTTACAT CTGGGAAATT ACTGAAGAAA TAGGACCCAA GGAATATGAC 
CCAGCTTGTC TCACAAATAT CTATTACTCC CATGAAAATA TGGTTCAAGA TTTTAACTCT 
GGACTGATTG GTGCTTTGCT TATCTGTAAA AAAGGAAGTT TAAATGAAGT TGGGGAGCAA 
CGCCATTTCG ATAAAGAGTA TGTGCTGATG TTTGCTGTAT TTGATGAAAA CAAAAGCTGG 
CAAAATTTGA ACGATAAAGA ACACTCTGTG ATGTATTCTA TAAATGGATA CGTTAATGGC 
ACAATTCCAG ATATCTATGC CTGTGCTGGG GACTCGGTTA GCTGGCATTT GTTAGGTATG 
AGCTCCGAAC CAGAATTTTT CTCTATTCAT TTCTTTGGTC AAACATTAGA AGAAAACCAA 
CATAAGGTTT CAGTAGTTGG CCTTGTTGCT TCTTCATCCA CCACAGCCAA CATGACAATA 
TACCAAACTG GGAAATGGTT AATAACATCC CTTGTTGAAA AGCATTTTGA GGCTGGGATG 
CATGGATACA TTGAAGTGCA GCCCTGCAGT GATAAGGGAT ACACAGCACC AAAATTATCA 
CTTCTTCAAA AGCGATTCAT AAAAGTTTGG GAGTACTTCA TAGCAGCAGA GGAAGAGGAG 
TGGAACTATG TCCCTTCAAA GGTAGAGAAC ACAGACAAGA TAAACTGCCA ACAAAAGTAT 
AAGAAAGTTC TCTACAAGGC ATATTCTGAT AACACATTCA CAAAGCTCTT GACAGCAGGG 
AATGAGGGCC TTCTTGGGCC TGTCATTAGA GCTCAGGTCA GAGATACAAT TACAGTTGTT 
TTCAAAAATA AGGCCAAACG GCCTTACAGC ATATACCCCC ATGGTGTGTC AGTACAAAAA 
GCCTATGAAG GTGCCAGTTA CCCCCCTGAT ATAAGAGGGA ATGAAACACA AAATCAAGCA 
GTAATGCCAG GAGAAACAGT GACGTACTAC TGGAATATTC TAGAAACTGA TGAACCTGCA 
GCACGGGATC CCCGTTGTTT AACAAGGATG TACCATAGTG CTGTTAATAT TACAAAAGAT 
ATTGCCTCTG GCTTAATCGG TCCGCTCCTC ATTTGCAAAT CAATGTCTCT AAATACAAGA 
GGTGTGCAGG AAAAGGCTGA TTTGGAACAA ATTGCAACAT TTGCTCTGTT TGATGAAAAC 
AAAAGTTGGT ACCATAATGA AAACAAGCTT AAGCATTGTC ATGCCGTTAA CAACGATCAA 
ACAGAGTACT ACAGCCATAA TATTATACCC ACCATAAATG GATATGCGTT TGAATCTGCG 
CCTTTGGGAT TCTGTCATAG CCAGGTGATC CACTGGCATA TTTCCAGCGT GGGTGCCCAA 
GATGAAATAA TTGGAGTACA TCTTTCAGGT CATACCCTCA GATACAAGGG AAAAAGTGAA 
GATACAGTAA ATATATTCCC GATGAGTGGT GAAAGTATTT CTGTAGATAT GGATAATCTT 
GGTTTATGGC TGTTTGGGGT GTTTGGGTCT TCTAAGAAAA ACCAGGGACT GAAAGTGAGA 
TTCACTGATG TCATCTGTGT AGAGGAAGAA GAAGATTATG AGGAACCAAA TATACAGTTT 
GGGATAAGTA GTGAAGAAAT TACAAAAAAA ATTGTTACAT CCCCTGAAAC CGACTACAGT 
GAGGAAGAAG ATGTAAACAC TAATTTAGAT ATAGACTACA CAGATAATTT AGCTGATTTG 
TTCCACATTC GGTCATTCAG AAACAAGACA GAAACACAGG AAGAAGGTGT AAATCTTACT 
GCTTTGGCCA TAGAGCACAC CTATGGGAGA CAAAATGAAA GCATGGGTGA CTTTAGTAAA 
CTTGAAGTTT CGGAGGAATA TGACATTGTT CCCATTGAGG ATAATGAAAA CTCAAATTTC 
AATCCTACGT CTGCAAGTAA AGCAGATAAA CTAACTAAAG ATGGCTTACC CAAAACACCT 
CAATACATGC AATCTAAAGA TGAAGATACC TTGGAGAATA CCTTGTCTGA GGAGTCTAAT 
ATGGCACAGA AAAATGTCAC AGTATATAAT CCTTTAGACA GTGAAAAAGA ACAATTTATC 
ATAATGGAGG GAAATCTTAC TACTGACAAA AACAGAGATC TATCATATGA CAGTTACTTT 
AATGAAGCAG ACAAAAATAT TTATAGTGAG GAAGACAAAG CTAAAATGAT CACTGAACAA 
AACACCTCAA ATTTGTTGGC GGAATCCAAA CCACAATTGA GCAATACAGA AAAAAGCCAT 
TTCATAAAAG AAGACCTATC ACACAGCATA AATAAAACAT TTTATTATTT GGTCACAACA 
TATGATAATG ACACACAAAA TTCAGAAACC CAGCATAATG AGTTTATGGC TTTGAAAGTT 
GCAGAAAAGA TTATTGATAC TCACCAAAAA GTTCCCAGGC AGGAGGAAAA AATGACAGAA 
ATTAGTGATC ATGTGACGAC TGTAGAGAAT AATACGTTAG ATATAACAAA GCATGATTTT 
CCTCTTAGAG AAGATCTTTT CAATGCCACT GATTCAAAGG AATACAATCC TCAGGTGGCA 
GACATAAAGG AAAAAAAATT GGTAAAAAGA TCCATTGAAC ACATGACAGA GAGTCCCACC 
TACTCTCCCT TAAATGTGGA TCATTTTCTG GAAACAGAAA ATGAAAAGTC ACATTCTTCC 
GATAATAAAG ACATCATGGA TGACTTCAGT ACAAATCAGG GACTTGACAG CAATGATACT 
TTTAATGATT TCATCAAAAA TCAGACAAGT GAAAGCAATT ACAATACCGA TAACATCAGC 
AAAAATCAGA CAGTTGCAAG CAGTGACATA ACTGACGACT TCAGCAAAAA TGGAACAACT 
AAAAACCTAC TATCATCAAC AAATATAACC AGTGGTATTA AAGAGCTACA TTCTTCAAAA 
AATAATGACC TTACTGATTA TTTAGATCAC GACAAAACAC CTGAAAAATT AATCCCATAC 
TTTAATGACA GCAATTTAAA TCTGTTGCCT AACAGGACAT CGCATAGCCT GGAGGAGAGT 
AAGCTACCCA ATGGTAACAG TAACAATTAT GGAAATCAAA CTGCAGCAAC AAGGGATATT 
GACTCTCCTG AAACTAAATC GGACACTGAA TTTAGAGAAT TGTTTGGACA ACAGATTTTT 
GAAGGTATAA TGCAAGTCAT ATTCTATCAG AAGGAAGTAA ATGAAACAAA CATGGAGCAG 
CATCTTGTTA CAGAAGAAAA CTCTACTGAA AGTGTGCAAA ATGAGTCACA TACACTTTTT 
AATAACAAAA CACAAGAAGA ACAAAATGCA AAAAAACCCA CAAAGCGATA TTTTCAAGTG 
AGACCAATCA GGTACAGAAC TTCAAGTAAC GAAACTAGAA TGTTAACTCG AAGAAAAAAA 
AGACTGGGAA ACGTCAATGG ATCCCTAAGC AATGACACTG GTAATGATGG AGTGTTCTCT 
CCCAGAGGAA TGAGACCACA AATTATAATT GGACTTCCTG GACTTGATGA GGGTGATTAT 
GTTGAGCTTA ATGTAGATGA AATCGATGAA GATGTACATA TTAAAAAAGT TGAATATGAG 
GAACTTTACA AAACAGAAGC GCAAGAGTAC ACTAACCCAG ATAAGATAAT ACAGCAATAC 
CTGCGTAGTT TAAAAGGAAA AAAAAGAAAG TATTACATCA CTGCAGAAGA GGAACAATGG 
GATTACTACA ATCCTGCAAA AAGTTCTTTA ACAAAAGAAA GGCCAGACAG TGAAACAAGG 
ACTACCCAGT ACACAAAAGT ACGGTTTCGG CAATATCTTG ATTCCAGTTT TAGCAAGCCT 
GATGTTCAAG GGGAATATGA AGAACACTTA GGTATCCTTG GTCCTGTAAT CCGAGCTGAA 
GTTGATGATA TTATTCAGAT AACGTTTAAA AACTTAGCAT CACGTCCCTA TTCTCTTCAT 
GCACACGGGG TTTCTTATGA AAAATCATCT GAAGGATTTC AGTATGATGA TGGGACCCCA 
GACTGGCTTA AGAAAGATGA TGCGGTTAAA CCAGGTGAAA CCTATACCTA TGTTTGGTAT 
GCCACACACC AATCAGCACC TGAAAATGAA GGCTCAGATT GCAGAACATG GATCTATTAT 
TCTGGAGTTA ACCCGGAAAA AGATATACAT TCAGGACTTC TGGGCCCACT GCTGATCTGT 
AAAAGCGGAA CACTTCATAA ACATAGCAAC AGGCCATTGG ATGTACGAGA ATTTGTATTG 
CTATTTATGA CATTTGAAGA AGAGAAAAGC TGGTACTTTG AAAAAAACAA GAAAAAGACC 
TGCTTGGAAG TCATGGAAAA ACCTTTACAA GCAGCAAAGT GTCATACATT TCACGCTATA 
AATGGCAAAA TCTACAACCT GAAAGGCTTA GTGATGTATC AGAATGAATC AGTGCGATGG 
CATATGATAA ATATGGGAGG TTCAAAGGAT TTACATGTGG TTCATTTCCA TGGTCAGACT 
GTGACAGAGA GGATTAACAG AGAGTCTCAA CGCGGTGTCT ACCCTCTTCT ACCTGGTTCA 
TTTGCAACAG TGGAAATGAA GCCATCGAAG CCTGGACATT GGCTCTTGGA TACAGAAGTT 
GCTGAGTACC AACAAGCAGG AATGCAGGCA ATGTTTCACA TTGTTGAGGC TGAATGCAAT 
TTACCAATGG GACTTTCAAA TGGAATTATA TCTGACAATC AAATCACTGC ATCACACTAT 
ATAGATTATT GGGAGCCCAG ACTGGCTAGG TTAAACAATG CAGGATCTTA CAATGCTTGG 
AGCACTGTAA TGAATATGTC AGCACTACCA TGGATTCAGG TGGATTTGCA AAGGCCTTTT 
TTAATAACAG GTATTAAAAC ACAGGGAGCA AGTAAATATC TTAAGCCATA TTACGTCAAA 
GAATTCTTTG TTGTGTACAG TTTGGATAAG AAGAATTGGA TAGCTTTTAA AGGAAACAGT 
ATTACACTGC AAATGTTCTT TGATGGTAAT ACCGATGCAC ACAGTATTAA GGAAAACACA 
TTGGACCCAC CAATTTCTGC CCAGTACATA AGAGTATATC CAACCAAATA CTACAACAGA 
CCAACTCTTC GTATGGAGCT ATTTGGGTGT GAAATACTAG GATGCTTTAT GCCGCTGGGC 
ATGGAAAACG GTATTATAAA GGATGAGCAG ATCACAGCTT CTTCTTATAA GAGTTCATGG 
TATTCCTCAT GGGTGCCATC TTTGGCTCGT CTTAATAAAG TAGGCAAATC CAATGCCTGG 
CAAGCAAAGT CAAATAACAA CCAACAGTGG CTACAAATTG ACTTTTTGAT GACAAAGAGG 
ATAACTGGTA TAACAACACA GGGGGCCAAG GCATTGACAA CTGATATGTA TGTAAAATCT 
TACAGTATCC AGTACAGTGA CAATGGGAAA GACTGGAAAC CATACACTGA TGAATTAGCA 
CCATTAGAAA AGGTATTCCT AGGAAATGCA AACAGCTATG GACTTGTCAA GAATGAAATA 
AGATCACCAA TATTGGCACG TTTCTTAAGG ATTATCCCTA AGAGCTGGCA CAACAGCATC 
ACACTGAGAA TAGAAATCTT TGGTTGTAAT ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935820.2
CDS	238..6273
Translation	MGRKRILSSSLFILFLLGTVLQSAPKVSAAVREYYIAAVITDWSYTNQYHDSSEFLYKKIIYREYEEGFQKAKPAVEYSGILGPTLRAEVGDILQVHFKNMANKPLTIHPQGVAYGKKSEGAKYTDSTLPYEKLDDSVMPGQMYTYIWEITEEIGPKEYDPACLTNIYYSHENMVQDFNSGLIGALLICKKGSLNEVGEQRHFDKEYVLMFAVFDENKSWQNLNDKEHSVMYSINGYVNGTIPDIYACAGDSVSWHLLGMSSEPEFFSIHFFGQTLEENQHKVSVVGLVASSSTTANMTIYQTGKWLITSLVEKHFEAGMHGYIEVQPCSDKGYTAPKLSLLQKRFIKVWEYFIAAEEEEWNYVPSKVENTDKINCQQKYKKVLYKAYSDNTFTKLLTAGNEGLLGPVIRAQVRDTITVVFKNKAKRPYSIYPHGVSVQKAYEGASYPPDIRGNETQNQAVMPGETVTYYWNILETDEPAARDPRCLTRMYHSAVNITKDIASGLIGPLLICKSMSLNTRGVQEKADLEQIATFALFDENKSWYHNENKLKHCHAVNNDQTEYYSHNIIPTINGYAFESAPLGFCHSQVIHWHISSVGAQDEIIGVHLSGHTLRYKGKSEDTVNIFPMSGESISVDMDNLGLWLFGVFGSSKKNQGLKVRFTDVICVEEEEDYEEPNIQFGISSEEITKKIVTSPETDYSEEEDVNTNLDIDYTDNLADLFHIRSFRNKTETQEEGVNLTALAIEHTYGRQNESMGDFSKLEVSEEYDIVPIEDNENSNFNPTSASKADKLTKDGLPKTPQYMQSKDEDTLENTLSEESNMAQKNVTVYNPLDSEKEQFIIMEGNLTTDKNRDLSYDSYFNEADKNIYSEEDKAKMITEQNTSNLLAESKPQLSNTEKSHFIKEDLSHSINKTFYYLVTTYDNDTQNSETQHNEFMALKVAEKIIDTHQKVPRQEEKMTEISDHVTTVENNTLDITKHDFPLREDLFNATDSKEYNPQVADIKEKKLVKRSIEHMTESPTYSPLNVDHFLETENEKSHSSDNKDIMDDFSTNQGLDSNDTFNDFIKNQTSESNYNTDNISKNQTVASSDITDDFSKNGTTKNLLSSTNITSGIKELHSSKNNDLTDYLDHDKTPEKLIPYFNDSNLNLLPNRTSHSLEESKLPNGNSNNYGNQTAATRDIDSPETKSDTEFRELFGQQIFEGIMQVIFYQKEVNETNMEQHLVTEENSTESVQNESHTLFNNKTQEEQNAKKPTKRYFQVRPIRYRTSSNETRMLTRRKKRLGNVNGSLSNDTGNDGVFSPRGMRPQIIIGLPGLDEGDYVELNVDEIDEDVHIKKVEYEELYKTEAQEYTNPDKIIQQYLRSLKGKKRKYYITAEEEQWDYYNPAKSSLTKERPDSETRTTQYTKVRFRQYLDSSFSKPDVQGEYEEHLGILGPVIRAEVDDIIQITFKNLASRPYSLHAHGVSYEKSSEGFQYDDGTPDWLKKDDAVKPGETYTYVWYATHQSAPENEGSDCRTWIYYSGVNPEKDIHSGLLGPLLICKSGTLHKHSNRPLDVREFVLLFMTFEEEKSWYFEKNKKKTCLEVMEKPLQAAKCHTFHAINGKIYNLKGLVMYQNESVRWHMINMGGSKDLHVVHFHGQTVTERINRESQRGVYPLLPGSFATVEMKPSKPGHWLLDTEVAEYQQAGMQAMFHIVEAECNLPMGLSNGIISDNQITASHYIDYWEPRLARLNNAGSYNAWSTVMNMSALPWIQVDLQRPFLITGIKTQGASKYLKPYYVKEFFVVYSLDKKNWIAFKGNSITLQMFFDGNTDAHSIKENTLDPPISAQYIRVYPTKYYNRPTLRMELFGCEILGCFMPLGMENGIIKDEQITASSYKSSWYSSWVPSLARLNKVGKSNAWQAKSNNNQQWLQIDFLMTKRITGITTQGAKALTTDMYVKSYSIQYSDNGKDWKPYTDELAPLEKVFLGNANSYGLVKNEIRSPILARFLRIIPKSWHNSITLRIEIFGCNI

Target ORF information:

RefSeq Version	XM_002935774.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis coagulation factor V (proaccelerin, labile factor) (f5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935774.4

ORF Insert Sequence:

ATGGGCCGGA AGAGAATTCT CTCCTCTTCT TTGTTCATTT TGTTCCTTCT GGGCACTGTA 
TTGCAGTCTG CTCCCAAAGT CTCAGCGGCT GTGAGGGAAT ATTATATTGC TGCTGTGATC 
ACAGACTGGA GCTACACCAA TCAATATCAT GACAGTTCAG AATTTCTATA CAAGAAGATT 
ATTTATAGAG AATATGAAGA AGGATTTCAA AAGGCAAAAC CAGCTGTTGA GTATTCAGGT 
ATTCTGGGGC CTACATTACG TGCAGAAGTT GGAGACATAC TACAAGTTCA TTTTAAAAAT 
ATGGCTAATA AACCGCTGAC TATTCATCCT CAAGGTGTTG CTTATGGGAA GAAATCAGAA 
GGGGCCAAAT ATACAGACAG TACTTTACCT TATGAAAAGC TGGATGACTC TGTTATGCCA 
GGACAAATGT ACACTTACAT CTGGGAAATT ACTGAAGAAA TAGGACCCAA GGAATATGAC 
CCAGCTTGTC TCACAAATAT CTATTACTCC CATGAAAATA TGGTTCAAGA TTTTAACTCT 
GGACTGATTG GTGCTTTGCT TATCTGTAAA AAAGGAAGTT TAAATGAAGT TGGGGAGCAA 
CGCCATTTCG ATAAAGAGTA TGTGCTGATG TTTGCTGTAT TTGATGAAAA CAAAAGCTGG 
CAAAATTTGA ACGATAAAGA ACACTCTGTG ATGTATTCTA TAAATGGATA CGTTAATGGC 
ACAATTCCAG ATATCTATGC CTGTGCTGGG GACTCGGTTA GCTGGCATTT GTTAGGTATG 
AGCTCCGAAC CAGAATTTTT CTCTATTCAT TTCTTTGGTC AAACATTAGA AGAAAACCAA 
CATAAGGTTT CAGTAGTTGG CCTTGTTGCT TCTTCATCCA CCACAGCCAA CATGACAATA 
TACCAAACTG GGAAATGGTT AATAACATCC CTTGTTGAAA AGCATTTTGA GGCTGGGATG 
CATGGATACA TTGAAGTGCA GCCCTGCAGT GATAAGGGAT ACACAGCACC AAAATTATCA 
CTTCTTCAAA AGCGATTCAT AAAAGTTTGG GAGTACTTCA TAGCAGCAGA GGAAGAGGAG 
TGGAACTATG TCCCTTCAAA GGTAGAGAAC ACAGACAAGA TAAACTGCCA ACAAAAGTAT 
AAGAAAGTTC TCTACAAGGC ATATTCTGAT AACACATTCA CAAAGCTCTT GACAGCAGGG 
AATGAGGGCC TTCTTGGGCC TGTCATTAGA GCTCAGGTCA GAGATACAAT TACAGTTGTT 
TTCAAAAATA AGGCCAAACG GCCTTACAGC ATATACCCCC ATGGTGTGTC AGTACAAAAA 
GCCTATGAAG GTGCCAGTTA CCCCCCTGAT ATAAGAGGGA ATGAAACACA AAATCAAGCA 
GTAATGCCAG GAGAAACAGT GACGTACTAC TGGAATATTC TAGAAACTGA TGAACCTGCA 
GCACGGGATC CCCGTTGTTT AACAAGGATG TACCATAGTG CTGTTAATAT TACAAAAGAT 
ATTGCCTCTG GCTTAATCGG TCCGCTCCTC ATTTGCAAAT CAATGTCTCT AAATACAAGA 
GGTGTGCAGG AAAAGGCTGA TTTGGAACAA ATTGCAACAT TTGCTCTGTT TGATGAAAAC 
AAAAGTTGGT ACCATAATGA AAACAAGCTT AAGCATTGTC ATGCCGTTAA CAACGATCAA 
ACAGAGTACT ACAGCCATAA TATTATACCC ACCATAAATG GATATGCGTT TGAATCTGCG 
CCTTTGGGAT TCTGTCATAG CCAGGTGATC CACTGGCATA TTTCCAGCGT GGGTGCCCAA 
GATGAAATAA TTGGAGTACA TCTTTCAGGT CATACCCTCA GATACAAGGG AAAAAGTGAA 
GATACAGTAA ATATATTCCC GATGAGTGGT GAAAGTATTT CTGTAGATAT GGATAATCTT 
GGTTTATGGC TGTTTGGGGT GTTTGGGTCT TCTAAGAAAA ACCAGGGACT GAAAGTGAGA 
TTCACTGATG TCATCTGTGT AGAGGAAGAA GAAGATTATG AGGAACCAAA TATACAGTTT 
GGGATAAGTA GTGAAGAAAT TACAAAAAAA ATTGTTACAT CCCCTGAAAC CGACTACAGT 
GAGGAAGAAG ATGTAAACAC TAATTTAGAT ATAGACTACA CAGATAATTT AGCTGATTTG 
TTCCACATTC GGTCATTCAG AAACAAGACA GAAACACAGG AAGAAGGTGT AAATCTTACT 
GCTTTGGCCA TAGAGCACAC CTATGGGAGA CAAAATGAAA GCATGGGTGA CTTTAGTAAA 
CTTGAAGTTT CGGAGGAATA TGACATTGTT CCCATTGAGG ATAATGAAAA CTCAAATTTC 
AATCCTACGT CTGCAAGTAA AGCAGATAAA CTAACTAAAG ATGGCTTACC CAAAACACCT 
CAATACATGC AATCTAAAGA TGAAGATACC TTGGAGAATA CCTTGTCTGA GGAGTCTAAT 
ATGGCACAGA AAAATGTCAC AGTATATAAT CCTTTAGACA GTGAAAAAGA ACAATTTATC 
ATAATGGAGG GAAATCTTAC TACTGACAAA AACAGAGATC TATCATATGA CAGTTACTTT 
AATGAAGCAG ACAAAAATAT TTATAGTGAG GAAGACAAAG CTAAAATGAT CACTGAACAA 
AACACCTCAA ATTTGTTGGC GGAATCCAAA CCACAATTGA GCAATACAGA AAAAAGCCAT 
TTCATAAAAG AAGACCTATC ACACAGCATA AATAAAACAT TTTATTATTT GGTCACAACA 
TATGATAATG ACACACAAAA TTCAGAAACC CAGCATAATG AGTTTATGGC TTTGAAAGTT 
GCAGAAAAGA TTATTGATAC TCACCAAAAA GTTCCCAGGC AGGAGGAAAA AATGACAGAA 
ATTAGTGATC ATGTGACGAC TGTAGAGAAT AATACGTTAG ATATAACAAA GCATGATTTT 
CCTCTTAGAG AAGATCTTTT CAATGCCACT GATTCAAAGG AATACAATCC TCAGGTGGCA 
GACATAAAGG AAAAAAAATT GGTAAAAAGA TCCATTGAAC ACATGACAGA GAGTCCCACC 
TACTCTCCCT TAAATGTGGA TCATTTTCTG GAAACAGAAA ATGAAAAGTC ACATTCTTCC 
GATAATAAAG ACATCATGGA TGACTTCAGT ACAAATCAGG GACTTGACAG CAATGATACT 
TTTAATGATT TCATCAAAAA TCAGACAAGT GAAAGCAATT ACAATACCGA TAACATCAGC 
AAAAATCAGA CAGTTGCAAG CAGTGACATA ACTGACGACT TCAGCAAAAA TGGAACAACT 
AAAAACCTAC TATCATCAAC AAATATAACC AGTGGTATTA AAGAGCTACA TTCTTCAAAA 
AATAATGACC TTACTGATTA TTTAGATCAC GACAAAACAC CTGAAAAATT AATCCCATAC 
TTTAATGACA GCAATTTAAA TCTGTTGCCT AACAGGACAT CGCATAGCCT GGAGGAGAGT 
AAGCTACCCA ATGGTAACAG TAACAATTAT GGAAATCAAA CTGCAGCAAC AAGGGATATT 
GACTCTCCTG AAACTAAATC GGACACTGAA TTTAGAGAAT TGTTTGGACA ACAGATTTTT 
GAAGGTATAA TGCAAGTCAT ATTCTATCAG AAGGAAGTAA ATGAAACAAA CATGGAGCAG 
CATCTTGTTA CAGAAGAAAA CTCTACTGAA AGTGTGCAAA ATGAGTCACA TACACTTTTT 
AATAACAAAA CACAAGAAGA ACAAAATGCA AAAAAACCCA CAAAGCGATA TTTTCAAGTG 
AGACCAATCA GGTACAGAAC TTCAAGTAAC GAAACTAGAA TGTTAACTCG AAGAAAAAAA 
AGACTGGGAA ACGTCAATGG ATCCCTAAGC AATGACACTG GTAATGATGG AGTGTTCTCT 
CCCAGAGGAA TGAGACCACA AATTATAATT GGACTTCCTG GACTTGATGA GGGTGATTAT 
GTTGAGCTTA ATGTAGATGA AATCGATGAA GATGTACATA TTAAAAAAGT TGAATATGAG 
GAACTTTACA AAACAGAAGC GCAAGAGTAC ACTAACCCAG ATAAGATAAT ACAGCAATAC 
CTGCGTAGTT TAAAAGGAAA AAAAAGAAAG TATTACATCA CTGCAGAAGA GGAACAATGG 
GATTACTACA ATCCTGCAAA AAGTTCTTTA ACAAAAGAAA GGCCAGACAG TGAAACAAGG 
ACTACCCAGT ACACAAAAGT ACGGTTTCGG CAATATCTTG ATTCCAGTTT TAGCAAGCCT 
GATGTTCAAG GGGAATATGA AGAACACTTA GGTATCCTTG GTCCTGTAAT CCGAGCTGAA 
GTTGATGATA TTATTCAGAT AACGTTTAAA AACTTAGCAT CACGTCCCTA TTCTCTTCAT 
GCACACGGGG TTTCTTATGA AAAATCATCT GAAGGATTTC AGTATGATGA TGGGACCCCA 
GACTGGCTTA AGAAAGATGA TGCGGTTAAA CCAGGTGAAA CCTATACCTA TGTTTGGTAT 
GCCACACACC AATCAGCACC TGAAAATGAA GGCTCAGATT GCAGAACATG GATCTATTAT 
TCTGGAGTTA ACCCGGAAAA AGATATACAT TCAGGACTTC TGGGCCCACT GCTGATCTGT 
AAAAGCGGAA CACTTCATAA ACATAGCAAC AGGCCATTGG ATGTACGAGA ATTTGTATTG 
CTATTTATGA CATTTGAAGA AGAGAAAAGC TGGTACTTTG AAAAAAACAA GAAAAAGACC 
TGCTTGGAAG TCATGGAAAA ACCTTTACAA GCAGCAAAGT GTCATACATT TCACGCTATA 
AATGGCAAAA TCTACAACCT GAAAGGCTTA GTGATGTATC AGAATGAATC AGTGCGATGG 
CATATGATAA ATATGGGAGG TTCAAAGGAT TTACATGTGG TTCATTTCCA TGGTCAGACT 
GTGACAGAGA GGATTAACAG AGAGTCTCAA CGCGGTGTCT ACCCTCTTCT ACCTGGTTCA 
TTTGCAACAG TGGAAATGAA GCCATCGAAG CCTGGACATT GGCTCTTGGA TACAGAAGTT 
GCTGAGTACC AACAAGCAGG AATGCAGGCA ATGTTTCACA TTGTTGAGGC TGAATGCAAT 
TTACCAATGG GACTTTCAAA TGGAATTATA TCTGACAATC AAATCACTGC ATCACACTAT 
ATAGATTATT GGGAGCCCAG ACTGGCTAGG TTAAACAATG CAGGATCTTA CAATGCTTGG 
AGCACTGTAA TGAATATGTC AGCACTACCA TGGATTCAGG TGGATTTGCA AAGGCCTTTT 
TTAATAACAG GTATTAAAAC ACAGGGAGCA AGTAAATATC TTAAGCCATA TTACGTCAAA 
GAATTCTTTG TTGTGTACAG TTTGGATAAG AAGAATTGGA TAGCTTTTAA AGGAAACAGT 
ATTACACTGC AAATGTTCTT TGATGGTAAT ACCGATGCAC ACAGTATTAA GGAAAACACA 
TTGGACCCAC CAATTTCTGC CCAGTACATA AGAGTATATC CAACCAAATA CTACAACAGA 
CCAACTCTTC GTATGGAGCT ATTTGGGTGT GAAATACTAG GATGCTTTAT GCCGCTGGGC 
ATGGAAAACG GTATTATAAA GGATGAGCAG ATCACAGCTT CTTCTTATAA GAGTTCATGG 
TATTCCTCAT GGGTGCCATC TTTGGCTCGT CTTAATAAAG TAGGCAAATC CAATGCCTGG 
CAAGCAAAGT CAAATAACAA CCAACAGTGG CTACAAATTG ACTTTTTGAT GACAAAGAGG 
ATAACTGGTA TAACAACACA GGGGGCCAAG GCATTGACAA CTGATATGTA TGTAAAATCT 
TACAGTATCC AGTACAGTGA CAATGGGAAA GACTGGAAAC CATACACTGA TGAATTAGCA 
CCATTAGAAA AGGTATTCCT AGGAAATGCA AACAGCTATG GACTTGTCAA GAATGAAATA 
AGATCACCAA TATTGGCACG TTTCTTAAGG ATTATCCCTA AGAGCTGGCA CAACAGCATC 
ACACTGAGAA TAGAAATCTT TGGTTGTAAT ATTTAA