bmp4.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmp4.L
Entrez Gene ID	397874
Full Name	bone morphogenetic protein 4 L homeolog
Synonyms	DVR-4,XBMP-4,bmp-4,bmp2b,bmp2b1,bmp4,bmp4-a,bmp4-b,ofc11,xbmp4,zyme
General protein information	Preferred Names bone morphogenetic protein 4 L homeolog Names bone morphogenetic protein 4 bone morphogenetic protein-4
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001088032.1	NP_001081501.1	bone morphogenetic protein 4 precursor
XM_018228698.1	XP_018084187.1	bone morphogenetic protein 4 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Suppression of Bmp4 signaling by the zinc-finger repressors Osr1 and Osr2 is required for Wnt/?-catenin-mediated lung specification in Xenopus.
Rankin SA, Gallas AL, Neto A, G?mez-Skarmeta JL, Zorn AM
Development (Cambridge, England)139(16)3010-20(2012 Aug)

?Np63 is regulated by BMP4 signaling and is required for early epidermal development in Xenopus.
Tr?bulo C, Guadalupe Barrionuevo M, Ag?ero TH, S?nchez SS, Calcaterra NB, Aybar MJ
Developmental dynamics : an official publication of the American Association of Anatomists241(2)257-69(2012 Feb)

Regulation of early Xenopus development by the PIAS genes.
Burn B, Brown S, Chang C
Developmental dynamics : an official publication of the American Association of Anatomists240(9)2120-6(2011 Sep)

Negative feedback in the bone morphogenetic protein 4 (BMP4) synexpression group governs its dynamic signaling range and canalizes development.
Paulsen M, Legewie S, Eils R, Karaulanov E, Niehrs C
Proceedings of the National Academy of Sciences of the United States of America108(25)10202-7(2011 Jun)

Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development.
Lee HS, Lee SY, Lee H, Hwang YS, Cha SW, Park S, Lee JY, Park JB, Kim S, Park MJ, Kim J
PloS one6(8)e22621(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Osr1/Osr2 normally repress bmp4 expression in the lateral plate mesoderm prior to respiratory specification.
Title: Suppression of Bmp4 signaling by the zinc-finger repressors Osr1 and Osr2 is required for Wnt/?-catenin-mediated lung specification in Xenopus.

The results suggest that DeltaNp63 is an essential gene in early epidermal specification under the control of BMP4.
Title: ?Np63 is regulated by BMP4 signaling and is required for early epidermal development in Xenopus.

PIAS proteins have differential ability to regulate signals from the growth factors activin, bone morphogenetic protein 4 (BMP4), and Wnt8.
Title: Regulation of early Xenopus development by the PIAS genes.

Data suggest that the feedback inhibitors BAMBI, SMAD6, and SMAD7 expand the dynamic BMP4 signaling range essential for proper embryonic patterning and reduce interindividual phenotypic and molecular variability in Xenopus embryos.
Title: Negative feedback in the bone morphogenetic protein 4 (BMP4) synexpression group governs its dynamic signaling range and canalizes development.

Data show that PV.1A undergoes combinatorial regulation during early Xenopus development as both the direct target of BMP-4 signaling and as the direct and indirect target of positive and negative regulatory factors.
Title: Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development.

The following bmp4.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp4.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf03837	NM_001088032.1 Latest version!	Xenopus laevis bone morphogenetic protein 4 L homeolog (bmp4.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OAf40990	XM_018228698.1 Latest version!	Xenopus laevis bone morphogenetic protein 4 L homeolog (bmp4.L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp4.L ( NM_001088032.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081501.1 Xenopus laevis bone morphogenetic protein 4 L homeolog (bmp4.L), mRNA.

Price & Availability↑

CloneID	OAf03837
Clone ID Related Accession (Same CDS sequence)	NM_001088032.1
Accession Version	NM_001088032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus laevis(African clawed frog)
Product	bone morphogenetic protein 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ005076.1. On Jul 25, 2007 this sequence version replaced NM_001088033.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04157061 [ECO:0000348] ##Evidence-Data-END##

ATGATTCCTG GTAACCGAAT GCTGATGGTC ATTTTATTAT GCCAAGTCCT GCTCGGAGGC 
ACTAACCATG CCAGCCTCAT ACCTGACACG GGCAAGAAGA AAGTAGCGGC CGACATTCAG 
GGGGGTCGGA GGTCCGCTCA GGGCCATGAG CTCTTGCGGG ATTTCGAGGT GACGCTGCTG 
CAGATGTTCG GCCTCGGCAA GCGGCCGCAG CCCAGTAAGG ATGTGGTGGT GCCCGCGTAT 
ATGCGCGACC TGTACAGGCT TCAGTCGGCG GAGGAAGAGG ATGAGCTGCA CGATATCAGC 
ATGGAGTATC CTGAGAGACC GACCAGCCGC GCCAACACTG TGAGGAGCTT CCATCACGAG 
GAGCATTTGG AGAATCTACC AAGCACAGCA GAAAATGGAA ATTTCCGTTT TGTGTTCAAC 
CTCAGCAGCA TTCCAGAGAA TGAGGTGATT TCTTCAGCAG AACTGAGACT CTATAGAGAA 
CAAATAGACC ATGGTCCAGC GTGGGATGAG GGTTTCCACC GGATAAATAT ATATGAAGTT 
ATGAAACCCA TAGCTGCAAA TGGACTCATG ATAAATAGGC TGCTGGACAC GAGACTAATC 
CACCACAATG TGACACAGTG GGAAAGTTTT GATGTAAGCC CTGCAATTAT GAGGTGGACC 
CGGGATAAAC AGATAAACCA TGGGCTTGCC ATTGAGGTCA TTCACCTCAA CCAAACCAAA 
ACTCATCAGG GGAAGCATGT ACGGATAAGT CGATCATTAT TACCTCAAGA GGATGCAGAC 
TGGTCGCAGA TGAGACCACT TTTAATTACA TTCAGCCATG ATGGCAGGGG ACATGCACTG 
ACCAGGAGGT CAAAAAGAAG TCCAAAACAG CAGAGACCCC GTAAAAAAAA CAAACATTGC 
CGGAGGCATT CTCTTTATGT GGATTTCAGC GATGTGGGCT GGAATGACTG GATTGTGGCA 
CCTCCTGGAT ACCAGGCCTT TTACTGCCAT GGAGATTGTC CATTTCCCTT GGCTGATCAC 
CTAAACTCAA CTAACCATGC TATTGTACAA ACTCTGGTAA ACTCTGTTAA CGCAAGCATC 
CCAAAAGCGT GTTGCGTCCC CACAGAACTG AGTGCTATCT CCATGCTTTA TTTGGACGAA 
TATGACAAAG TCGTCCTTAA AAACTACCAG GAGATGGTGG TGGAAGGGTG TGGGTGCCGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081501.1
CDS	156..1358
Translation	MIPGNRMLMVILLCQVLLGGTNHASLIPDTGKKKVAADIQGGRRSAQGHELLRDFEVTLLQMFGLGKRPQPSKDVVVPAYMRDLYRLQSAEEEDELHDISMEYPERPTSRANTVRSFHHEEHLENLPSTAENGNFRFVFNLSSIPENEVISSAELRLYREQIDHGPAWDEGFHRINIYEVMKPIAANGLMINRLLDTRLIHHNVTQWESFDVSPAIMRWTRDKQINHGLAIEVIHLNQTKTHQGKHVRISRSLLPQEDADWSQMRPLLITFSHDGRGHALTRRSKRSPKQQRPRKKNKHCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNASIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

Target ORF information:

RefSeq Version	NM_001088032.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis bone morphogenetic protein 4 L homeolog (bmp4.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001088032.1

ORF Insert Sequence:

ATGATTCCTG GTAACCGAAT GCTGATGGTC ATTTTATTAT GCCAAGTCCT GCTCGGAGGC 
ACTAACCATG CCAGCCTCAT ACCTGACACG GGCAAGAAGA AAGTAGCGGC CGACATTCAG 
GGGGGTCGGA GGTCCGCTCA GGGCCATGAG CTCTTGCGGG ATTTCGAGGT GACGCTGCTG 
CAGATGTTCG GCCTCGGCAA GCGGCCGCAG CCCAGTAAGG ATGTGGTGGT GCCCGCGTAT 
ATGCGCGACC TGTACAGGCT TCAGTCGGCG GAGGAAGAGG ATGAGCTGCA CGATATCAGC 
ATGGAGTATC CTGAGAGACC GACCAGCCGC GCCAACACTG TGAGGAGCTT CCATCACGAG 
GAGCATTTGG AGAATCTACC AAGCACAGCA GAAAATGGAA ATTTCCGTTT TGTGTTCAAC 
CTCAGCAGCA TTCCAGAGAA TGAGGTGATT TCTTCAGCAG AACTGAGACT CTATAGAGAA 
CAAATAGACC ATGGTCCAGC GTGGGATGAG GGTTTCCACC GGATAAATAT ATATGAAGTT 
ATGAAACCCA TAGCTGCAAA TGGACTCATG ATAAATAGGC TGCTGGACAC GAGACTAATC 
CACCACAATG TGACACAGTG GGAAAGTTTT GATGTAAGCC CTGCAATTAT GAGGTGGACC 
CGGGATAAAC AGATAAACCA TGGGCTTGCC ATTGAGGTCA TTCACCTCAA CCAAACCAAA 
ACTCATCAGG GGAAGCATGT ACGGATAAGT CGATCATTAT TACCTCAAGA GGATGCAGAC 
TGGTCGCAGA TGAGACCACT TTTAATTACA TTCAGCCATG ATGGCAGGGG ACATGCACTG 
ACCAGGAGGT CAAAAAGAAG TCCAAAACAG CAGAGACCCC GTAAAAAAAA CAAACATTGC 
CGGAGGCATT CTCTTTATGT GGATTTCAGC GATGTGGGCT GGAATGACTG GATTGTGGCA 
CCTCCTGGAT ACCAGGCCTT TTACTGCCAT GGAGATTGTC CATTTCCCTT GGCTGATCAC 
CTAAACTCAA CTAACCATGC TATTGTACAA ACTCTGGTAA ACTCTGTTAA CGCAAGCATC 
CCAAAAGCGT GTTGCGTCCC CACAGAACTG AGTGCTATCT CCATGCTTTA TTTGGACGAA 
TATGACAAAG TCGTCCTTAA AAACTACCAG GAGATGGTGG TGGAAGGGTG TGGGTGCCGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp4.L ( XM_018228698.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018084187.1 Xenopus laevis bone morphogenetic protein 4 L homeolog (bmp4.L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf40990
Clone ID Related Accession (Same CDS sequence)	XM_018228698.1
Accession Version	XM_018228698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	bone morphogenetic protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030738.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTTTG TTTGTTGTGC ACCCTCCAGA GACATCATGA TTCCTGGTAA CCGAATGCTG 
ATGGTCATTT TATTATGCCA AGTCCTGCTC GGAGGCACTA ACCATGCCAG CCTCATACCT 
GACACGGGCA AGAAGAAAGT AGCGGCCGAC ATTCAGGGGG GTCGGAGGTC CGCTCAGGGC 
CATGAGCTCT TGCGGGATTT CGAGGTGACG CTGCTGCAGA TGTTCGGCCT CGGCAAGCGG 
CCGCAGCCCA GTAAGGATGT GGTGGTGCCC GCGTATATGC GCGACCTGTA CAGGCTTCAG 
TCGGCGGAGG AAGAGGATGA GCTGCACGAT ATCAGCATGG AGTATCCTGA GAGACCGACC 
AGCCGCGCCA ACACTGTGAG GAGCTTCCAT CACGAGGAGC ATTTGGAGAA TCTACCAAGC 
ACAGCAGAAA ATGGAAATTT CCGTTTTGTG TTCAACCTCA GCAGCATTCC AGAGAATGAG 
GTGATTTCTT CAGCAGAACT GAGACTCTAT AGAGAACAAA TAGACCATGG TCCAGCGTGG 
GATGAGGGTT TCCACCGGAT AAATATATAT GAAGTTATGA AACCCATAGC TGCAAATGGA 
CTCATGATAA ATAGGCTGCT GGACACGAGA CTAATCCACC ACAATGTGAC ACAGTGGGAA 
AGTTTTGATG TAAGCCCTGC AATTATGAGG TGGACCCGGG ATAAACAGAT AAACCATGGG 
CTTGCCATTG AGGTCATTCA CCTCAACCAA ACCAAAACTC ATCAGGGGAA GCATGTAAGG 
ATAAGTCGAT CATTATTACC TCAAGAGGAT GCAGACTGGT CGCAGATGAG ACCACTTTTA 
ATTACATTCA GCCATGATGG CAGGGGACAT GCACTGACCA GGAGGTCAAA AAGAAGTCCA 
AAACAGCAGA GACCCCGTAA AAAAAACAAA CATTGCCGGA GGCATTCTCT TTATGTGGAT 
TTCAGCGATG TGGGCTGGAA TGACTGGATT GTGGCACCTC CTGGATACCA GGCCTTTTAC 
TGCCATGGAG ATTGTCCATT TCCCTTGGCT GATCACCTAA ACTCAACTAA CCATGCTATT 
GTACAAACTC TGGTAAACTC TGTTAACGCA AGCATCCCAA AAGCGTGTTG CGTCCCCACA 
GAACTGAGTG CTATCTCCAT GCTTTATTTG GACGAATATG ACAAAGTCGT CCTTAAAAAC 
TACCAGGAGA TGGTGGTGGA AGGGTGTGGG TGCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018084187.1
CDS	981..2219
Translation	MYFVCCAPSRDIMIPGNRMLMVILLCQVLLGGTNHASLIPDTGKKKVAADIQGGRRSAQGHELLRDFEVTLLQMFGLGKRPQPSKDVVVPAYMRDLYRLQSAEEEDELHDISMEYPERPTSRANTVRSFHHEEHLENLPSTAENGNFRFVFNLSSIPENEVISSAELRLYREQIDHGPAWDEGFHRINIYEVMKPIAANGLMINRLLDTRLIHHNVTQWESFDVSPAIMRWTRDKQINHGLAIEVIHLNQTKTHQGKHVRISRSLLPQEDADWSQMRPLLITFSHDGRGHALTRRSKRSPKQQRPRKKNKHCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNASIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

Target ORF information:

RefSeq Version	XM_018228698.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis bone morphogenetic protein 4 L homeolog (bmp4.L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018228698.1

ORF Insert Sequence:

ATGTATTTTG TTTGTTGTGC ACCCTCCAGA GACATCATGA TTCCTGGTAA CCGAATGCTG 
ATGGTCATTT TATTATGCCA AGTCCTGCTC GGAGGCACTA ACCATGCCAG CCTCATACCT 
GACACGGGCA AGAAGAAAGT AGCGGCCGAC ATTCAGGGGG GTCGGAGGTC CGCTCAGGGC 
CATGAGCTCT TGCGGGATTT CGAGGTGACG CTGCTGCAGA TGTTCGGCCT CGGCAAGCGG 
CCGCAGCCCA GTAAGGATGT GGTGGTGCCC GCGTATATGC GCGACCTGTA CAGGCTTCAG 
TCGGCGGAGG AAGAGGATGA GCTGCACGAT ATCAGCATGG AGTATCCTGA GAGACCGACC 
AGCCGCGCCA ACACTGTGAG GAGCTTCCAT CACGAGGAGC ATTTGGAGAA TCTACCAAGC 
ACAGCAGAAA ATGGAAATTT CCGTTTTGTG TTCAACCTCA GCAGCATTCC AGAGAATGAG 
GTGATTTCTT CAGCAGAACT GAGACTCTAT AGAGAACAAA TAGACCATGG TCCAGCGTGG 
GATGAGGGTT TCCACCGGAT AAATATATAT GAAGTTATGA AACCCATAGC TGCAAATGGA 
CTCATGATAA ATAGGCTGCT GGACACGAGA CTAATCCACC ACAATGTGAC ACAGTGGGAA 
AGTTTTGATG TAAGCCCTGC AATTATGAGG TGGACCCGGG ATAAACAGAT AAACCATGGG 
CTTGCCATTG AGGTCATTCA CCTCAACCAA ACCAAAACTC ATCAGGGGAA GCATGTAAGG 
ATAAGTCGAT CATTATTACC TCAAGAGGAT GCAGACTGGT CGCAGATGAG ACCACTTTTA 
ATTACATTCA GCCATGATGG CAGGGGACAT GCACTGACCA GGAGGTCAAA AAGAAGTCCA 
AAACAGCAGA GACCCCGTAA AAAAAACAAA CATTGCCGGA GGCATTCTCT TTATGTGGAT 
TTCAGCGATG TGGGCTGGAA TGACTGGATT GTGGCACCTC CTGGATACCA GGCCTTTTAC 
TGCCATGGAG ATTGTCCATT TCCCTTGGCT GATCACCTAA ACTCAACTAA CCATGCTATT 
GTACAAACTC TGGTAAACTC TGTTAACGCA AGCATCCCAA AAGCGTGTTG CGTCCCCACA 
GAACTGAGTG CTATCTCCAT GCTTTATTTG GACGAATATG ACAAAGTCGT CCTTAAAAAC 
TACCAGGAGA TGGTGGTGGA AGGGTGTGGG TGCCGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Suppression of Bmp4 signaling by the zinc-finger repressors Osr1 and Osr2 is required for Wnt/?-catenin-mediated lung specification in Xenopus.
Development (Cambridge, England)139(16)3010-20(2012 Aug)
Rankin SA,Gallas AL,Neto A,G?mez-Skarmeta JL,Zorn AM

?Np63 is regulated by BMP4 signaling and is required for early epidermal development in Xenopus.
Developmental dynamics : an official publication of the American Association of Anatomists241(2)257-69(2012 Feb)
Tr?bulo C,Guadalupe Barrionuevo M,Ag?ero TH,S?nchez SS,Calcaterra NB,Aybar MJ

Regulation of early Xenopus development by the PIAS genes.
Developmental dynamics : an official publication of the American Association of Anatomists240(9)2120-6(2011 Sep)
Burn B,Brown S,Chang C

Negative feedback in the bone morphogenetic protein 4 (BMP4) synexpression group governs its dynamic signaling range and canalizes development.
Proceedings of the National Academy of Sciences of the United States of America108(25)10202-7(2011 Jun)
Paulsen M,Legewie S,Eils R,Karaulanov E,Niehrs C

Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development.
PloS one6(8)e22621(2011)
Lee HS,Lee SY,Lee H,Hwang YS,Cha SW,Park S,Lee JY,Park JB,Kim S,Park MJ,Kim J

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bmp4.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム