gdf3.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gdf3.S
Entrez Gene ID	394305
Full Name	growth differentiation factor 3 S homeolog
Synonyms	der,derriere,gdf3,gdf3-a,vgr-2,vgr2
General protein information	Preferred Names growth differentiation factor 3 S homeolog Names growth/differentiation factor 3 derriA re derriere (posterior determination, TGFb family member) derriere (tgf-beta family member) gdf-3
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001087497.1	NP_001080966.1	growth/differentiation factor 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The left-right axis is regulated by the interplay of Coco, Xnr1 and derri?re in Xenopus embryos.
Vonica A, Brivanlou AH
Developmental biology303(1)281-94(2007 Mar)

Visualizing long-range movement of the morphogen Xnr2 in the Xenopus embryo.
Williams PH, Hagemann A, Gonz?lez-Gait?n M, Smith JC
Current biology : CB14(21)1916-23(2004 Nov)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Direct and indirect regulation of derri?re, a Xenopus mesoderm-inducing factor, by VegT.
White RJ, Sun BI, Sive HL, Smith JC
Development (Cambridge, England)129(20)4867-76(2002 Oct)

Effects of heterodimerization and proteolytic processing on Derri?re and Nodal activity: implications for mesoderm induction in Xenopus.
Eimon PM, Harland RM
Development (Cambridge, England)129(13)3089-103(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Xnr1 and derriere are coexpressed in the posterior paraxial mesoderm at neurula stage, and with Coco define a posttranscriptionally regulated signaling center in the chain leading to an increased TGF-beta signal on the left side of the embryo.
Title: The left-right axis is regulated by the interplay of Coco, Xnr1 and derri?re in Xenopus embryos.

The following gdf3.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gdf3.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00705	NM_001087497.1 Latest version!	Xenopus laevis growth differentiation factor 3 S homeolog (gdf3.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gdf3.S ( NM_001087497.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001080966.1 Xenopus laevis growth differentiation factor 3 S homeolog (gdf3.S), mRNA.

Price & Availability↑

CloneID	OAf00705
Clone ID Related Accession (Same CDS sequence)	NM_001087497.1
Accession Version	NM_001087497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus laevis(African clawed frog)
Product	growth/differentiation factor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF065135.1. On or before Jul 25, 2007 this sequence version replaced NM_001087498.1, NM_001087499.1. ##Evidence-Data-START## Transcript exon combination :: AF065135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02851420, SAMN02908486 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGAGT TGTGGCTATC ACTTTCTTGC ATGTTCTCCT TGCTTCTACT GACAAATTCA 
TCTCCACTTA CCTTCCAGGA AAGAATGCTC CTTAAAGCCT TGGGGCTGAA CACCAGACCA 
AACCCCATTG CTCCAGCTCC TGTACCTAAA TCTTTAAGAG ACATTTTTGA GAAGGGGATA 
AACCAGGACA ATCCCTGCAT GATGGAAGGT TTCGGAGTAC CTGGAAATAT TGTCCGCTCA 
TATCGAGATC AAGGAACCAT AGCAGCCATA GAGGAGCCAC AAGGATCTCT GTGCTTAAAG 
AAATTTCTCT TTTTTGACCT ATCAGCAGTG GAGAACAAGG AGCAATTGAC CCTAGGCCAA 
CTGGAAATTA AGTTCAAGCA CAACACATAT TATGGACAAC AGTTCCATCT CCGCCTCTAC 
CGCACCCTTC AGCTATCTCT AAAAGGGATG AGAGACAGCA AGATGAACAG GAAGCTCCTG 
GTGACTCAGT CTTTCCGTCT CCTTCACAAG TCCCTCTATT TCAACTTGAC CAAGGTGGCA 
GAGGACTGGA AAAACCCTGA GAAGAATATG GGTCTGATAC TGGAAATATA TGCAAGCAGT 
GAACTTGCAG GAGGCAATCG ATCATTTGTA GTATGTGAAC CAATACAGTC TTTCATTTAC 
ACTTCTCTGC TCACTGTGTC CCTAGACCCA TCCAATTGCA AAACTCAACG AGCCAAGAGG 
AGTACTCATT CATCACCTCC AACCCCAAGC AATATCTGCA AGAAAAGGAG ATTGTACATT 
GACTTCAAGG ATGTTGGATG GCAGAACTGG GTCATTGCAC CCCGTGGTTA CATGGCAAAC 
TACTGCCATG GAGAGTGCCC CTATCCACTG ACGGAAATGC TAAGGGGCAC AAATCATGCT 
GTTTTACAGA CTCTGGTGCA TTCTGTAGAA CCAGAAAACA CCCCATTGCC TTGCTGTGCC 
CCCACTAAGC TGTCTCCTAT CTCCATGCTA TATTATGACA ACAATGACAA TGTGGTACTG 
AGGCACTATG AAGATATGGT AGTGGATGAG TGTGGTTGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001080966.1
CDS	55..1119
Translation	MAELWLSLSCMFSLLLLTNSSPLTFQERMLLKALGLNTRPNPIAPAPVPKSLRDIFEKGINQDNPCMMEGFGVPGNIVRSYRDQGTIAAIEEPQGSLCLKKFLFFDLSAVENKEQLTLGQLEIKFKHNTYYGQQFHLRLYRTLQLSLKGMRDSKMNRKLLVTQSFRLLHKSLYFNLTKVAEDWKNPEKNMGLILEIYASSELAGGNRSFVVCEPIQSFIYTSLLTVSLDPSNCKTQRAKRSTHSSPPTPSNICKKRRLYIDFKDVGWQNWVIAPRGYMANYCHGECPYPLTEMLRGTNHAVLQTLVHSVEPENTPLPCCAPTKLSPISMLYYDNNDNVVLRHYEDMVVDECGCK

Target ORF information:

RefSeq Version	NM_001087497.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis growth differentiation factor 3 S homeolog (gdf3.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001087497.1

ORF Insert Sequence:

ATGGCAGAGT TGTGGCTATC ACTTTCTTGC ATGTTCTCCT TGCTTCTACT GACAAATTCA 
TCTCCACTTA CCTTCCAGGA AAGAATGCTC CTTAAAGCCT TGGGGCTGAA CACCAGACCA 
AACCCCATTG CTCCAGCTCC TGTACCTAAA TCTTTAAGAG ACATTTTTGA GAAGGGGATA 
AACCAGGACA ATCCCTGCAT GATGGAAGGT TTCGGAGTAC CTGGAAATAT TGTCCGCTCA 
TATCGAGATC AAGGAACCAT AGCAGCCATA GAGGAGCCAC AAGGATCTCT GTGCTTAAAG 
AAATTTCTCT TTTTTGACCT ATCAGCAGTG GAGAACAAGG AGCAATTGAC CCTAGGCCAA 
CTGGAAATTA AGTTCAAGCA CAACACATAT TATGGACAAC AGTTCCATCT CCGCCTCTAC 
CGCACCCTTC AGCTATCTCT AAAAGGGATG AGAGACAGCA AGATGAACAG GAAGCTCCTG 
GTGACTCAGT CTTTCCGTCT CCTTCACAAG TCCCTCTATT TCAACTTGAC CAAGGTGGCA 
GAGGACTGGA AAAACCCTGA GAAGAATATG GGTCTGATAC TGGAAATATA TGCAAGCAGT 
GAACTTGCAG GAGGCAATCG ATCATTTGTA GTATGTGAAC CAATACAGTC TTTCATTTAC 
ACTTCTCTGC TCACTGTGTC CCTAGACCCA TCCAATTGCA AAACTCAACG AGCCAAGAGG 
AGTACTCATT CATCACCTCC AACCCCAAGC AATATCTGCA AGAAAAGGAG ATTGTACATT 
GACTTCAAGG ATGTTGGATG GCAGAACTGG GTCATTGCAC CCCGTGGTTA CATGGCAAAC 
TACTGCCATG GAGAGTGCCC CTATCCACTG ACGGAAATGC TAAGGGGCAC AAATCATGCT 
GTTTTACAGA CTCTGGTGCA TTCTGTAGAA CCAGAAAACA CCCCATTGCC TTGCTGTGCC 
CCCACTAAGC TGTCTCCTAT CTCCATGCTA TATTATGACA ACAATGACAA TGTGGTACTG 
AGGCACTATG AAGATATGGT AGTGGATGAG TGTGGTTGCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The left-right axis is regulated by the interplay of Coco, Xnr1 and derri?re in Xenopus embryos.
Developmental biology303(1)281-94(2007 Mar)
Vonica A,Brivanlou AH

Visualizing long-range movement of the morphogen Xnr2 in the Xenopus embryo.
Current biology : CB14(21)1916-23(2004 Nov)
Williams PH,Hagemann A,Gonz?lez-Gait?n M,Smith JC

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Direct and indirect regulation of derri?re, a Xenopus mesoderm-inducing factor, by VegT.
Development (Cambridge, England)129(20)4867-76(2002 Oct)
White RJ,Sun BI,Sive HL,Smith JC

Effects of heterodimerization and proteolytic processing on Derri?re and Nodal activity: implications for mesoderm induction in Xenopus.
Development (Cambridge, England)129(13)3089-103(2002 Jul)
Eimon PM,Harland RM