smad1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	smad1.L
Entrez Gene ID	379664
Full Name	SMAD family member 1 L homeolog
Synonyms	MLP,Xmad1,Xsmad1,smad-1,smad1,smad1-a,smad1-b,xmad
General protein information	Preferred Names SMAD family member 1 L homeolog Names SMAD family member 1 L homeolog mothers against DPP
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001086504.1	NP_001079973.1	SMAD family member 1 L homeolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Identification of microRNAs and microRNA targets in Xenopus gastrulae: The role of miR-26 in the regulation of Smad1.
Liu C, Lou CH, Shah V, Ritter R, Talley J, Soibam B, Benham A, Zhu H, Perez E, Shieh YE, Gunaratne PH, Sater AK
Developmental biology409(1)26-38(2016 Jan)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor.
Thomsen GH
Development (Cambridge, England)122(8)2359-66(1996 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interactions between miR-26 and the Smad1 3'UTR modulate Smad1 function in the establishment of axial patterning.
Title: Identification of microRNAs and microRNA targets in Xenopus gastrulae: The role of miR-26 in the regulation of Smad1.

The following smad1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smad1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00380	NM_001086504.1 Latest version!	Xenopus laevis SMAD family member 1 L homeolog (smad1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smad1.L ( NM_001086504.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001079973.1 Xenopus laevis SMAD family member 1 L homeolog (smad1.L), mRNA.

Price & Availability↑

CloneID	OAf00380
Clone ID Related Accession (Same CDS sequence)	NM_001086504.1
Accession Version	NM_001086504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus laevis(African clawed frog)
Product	SMAD family member 1 L homeolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057746.1. ##Evidence-Data-START## Transcript exon combination :: BC057746.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN04157050, SAMN04157051 [ECO:0000350] ##Evidence-Data-END##

ATGAATGTGA CAAGCCTTTT CTCCTTCACA AGCCCAGCTG TGAAGAGGCT GCTTGGTTGG 
AAACAGGGAG ACGAAGAAGA GAAATGGGCA GAGAAAGCGG TGGATGCCTT GGTGAAAAAG 
CTGAAGAAGA AAAAAGGAGC CATGGAAGAA CTTGAAAAGG CCCTGAGTTG TCCTGGACAG 
CCCAGTAACT GTGTCACCAT TCCTCGTTCC TTGGATGGCC GACTGCAAGT GTCTCACCGT 
AAGGGCCTAC CGCATGTCAT TTATTGCAGA GTGTGGCGTT GGCCTGATCT ACAGAGTCAC 
CATGAACTGA AACCCTTGGA GTGTTGCGAG TATCCCTTTG GTTCCAAACA GAAGGAGGTC 
TGCATCAACC CATATCATTA TAAACGAGTG GAGAGTCCTG TCTTGCCGCC TGTCCTTGTT 
CCACGGCACA GTGAGTACAA CCCTCAACAC AGTCTCCTTG CGCAATTCCG AAACTTGGAG 
CCAAGTGAGC CACACATGCC TCACAACGCA ACTTTTCCCG ACTCTTTCCA GCAGCCAAAC 
AGCCATCCGT TCCCTCACTC GCCCAACAGC AGCTACCCAA ACTCTCCGGG AAGCAGCAGT 
ACTTACCCTC ACTCACCGGC GAGCTCTGAT CCTGGAAGCC CTTTTCAAAT ACCAGCTGAC 
ACCCCTCCTC CAGCTTATAT GCCTCCTGAG GATCAGATGA CGCAAGACAA CTCTCAGCCG 
ATGGACACAA ATATGATGGT GCCTAACATC TCTCAAGATA TCAATAGAGC AGATGTACAA 
GCTGTTGCCT ATGAAGAGCC AAAACACTGG TGCTCCATTG TCTATTATGA GCTGAACAAC 
CGTGTAGGAG AAGCTTTCCA TGCCTCCTCC ACAAGTGTAT TGGTGGACGG CTTCACTGAT 
CCTTCAAACA ACAGGAACAG ATTTTGCCTT GGGCTTCTGT CCAATGTCAA CCGGAACTCG 
ACCATTGAGA ACACCAGGCG GCATATTGGG AAAGGTGTGC ATTTATACTA CGTCGGGGGT 
GAGGTCTATG CTGAATGCTT AAGCGACAGC AGCATTTTTG TTCAGAGTCG GAATTGTAAC 
TTCCACCATG GTTTTCATCC TACAACTGTG TGTAAAATCC CCAGCGGGTG CAGTCTGAAG 
ATCTTTAACA ACCAGGAATT TGCTCAGCTT TTGGCCCAGT CTGTAAACCA TGGCTTTGAA 
ACGGTCTATG AACTGACAAA GATGTGCACT ATTCGAATGA GTTTTGTCAA GGGCTGGGGT 
GCGGAATATC ATCGGCAGGA TGTGACAAGC ACCCCCTGCT GGATTGAGAT TCACCTTCAT 
GGGCCACTTC AATGGCTGGA TAAAGTACTT ACTCAGATGG GCTCACCCCA TAATCCCATC 
TCCTCTGTCT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001079973.1
CDS	259..1653
Translation	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEYPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLEPSEPHMPHNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSTYPHSPASSDPGSPFQIPADTPPPAYMPPEDQMTQDNSQPMDTNMMVPNISQDINRADVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNRNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNFHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Target ORF information:

RefSeq Version	NM_001086504.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis SMAD family member 1 L homeolog (smad1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001086504.1

ORF Insert Sequence:

ATGAATGTGA CAAGCCTTTT CTCCTTCACA AGCCCAGCTG TGAAGAGGCT GCTTGGTTGG 
AAACAGGGAG ACGAAGAAGA GAAATGGGCA GAGAAAGCGG TGGATGCCTT GGTGAAAAAG 
CTGAAGAAGA AAAAAGGAGC CATGGAAGAA CTTGAAAAGG CCCTGAGTTG TCCTGGACAG 
CCCAGTAACT GTGTCACCAT TCCTCGTTCC TTGGATGGCC GACTGCAAGT GTCTCACCGT 
AAGGGCCTAC CGCATGTCAT TTATTGCAGA GTGTGGCGTT GGCCTGATCT ACAGAGTCAC 
CATGAACTGA AACCCTTGGA GTGTTGCGAG TATCCCTTTG GTTCCAAACA GAAGGAGGTC 
TGCATCAACC CATATCATTA TAAACGAGTG GAGAGTCCTG TCTTGCCGCC TGTCCTTGTT 
CCACGGCACA GTGAGTACAA CCCTCAACAC AGTCTCCTTG CGCAATTCCG AAACTTGGAG 
CCAAGTGAGC CACACATGCC TCACAACGCA ACTTTTCCCG ACTCTTTCCA GCAGCCAAAC 
AGCCATCCGT TCCCTCACTC GCCCAACAGC AGCTACCCAA ACTCTCCGGG AAGCAGCAGT 
ACTTACCCTC ACTCACCGGC GAGCTCTGAT CCTGGAAGCC CTTTTCAAAT ACCAGCTGAC 
ACCCCTCCTC CAGCTTATAT GCCTCCTGAG GATCAGATGA CGCAAGACAA CTCTCAGCCG 
ATGGACACAA ATATGATGGT GCCTAACATC TCTCAAGATA TCAATAGAGC AGATGTACAA 
GCTGTTGCCT ATGAAGAGCC AAAACACTGG TGCTCCATTG TCTATTATGA GCTGAACAAC 
CGTGTAGGAG AAGCTTTCCA TGCCTCCTCC ACAAGTGTAT TGGTGGACGG CTTCACTGAT 
CCTTCAAACA ACAGGAACAG ATTTTGCCTT GGGCTTCTGT CCAATGTCAA CCGGAACTCG 
ACCATTGAGA ACACCAGGCG GCATATTGGG AAAGGTGTGC ATTTATACTA CGTCGGGGGT 
GAGGTCTATG CTGAATGCTT AAGCGACAGC AGCATTTTTG TTCAGAGTCG GAATTGTAAC 
TTCCACCATG GTTTTCATCC TACAACTGTG TGTAAAATCC CCAGCGGGTG CAGTCTGAAG 
ATCTTTAACA ACCAGGAATT TGCTCAGCTT TTGGCCCAGT CTGTAAACCA TGGCTTTGAA 
ACGGTCTATG AACTGACAAA GATGTGCACT ATTCGAATGA GTTTTGTCAA GGGCTGGGGT 
GCGGAATATC ATCGGCAGGA TGTGACAAGC ACCCCCTGCT GGATTGAGAT TCACCTTCAT 
GGGCCACTTC AATGGCTGGA TAAAGTACTT ACTCAGATGG GCTCACCCCA TAATCCCATC 
TCCTCTGTCT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of microRNAs and microRNA targets in Xenopus gastrulae: The role of miR-26 in the regulation of Smad1.
Developmental biology409(1)26-38(2016 Jan)
Liu C,Lou CH,Shah V,Ritter R,Talley J,Soibam B,Benham A,Zhu H,Perez E,Shieh YE,Gunaratne PH,Sater AK

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor.
Development (Cambridge, England)122(8)2359-66(1996 Aug)
Thomsen GH