LOC108702044 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108702044
Entrez Gene ID	108702044
Full Name	E3 ubiquitin/ISG15 ligase TRIM25-like
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018237052.1	XP_018092541.1	E3 ubiquitin/ISG15 ligase TRIM25-like

Pathways from BioSystems
Gene Ontology

The following LOC108702044 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108702044 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf47274	XM_018237052.1 Latest version!	Xenopus laevis E3 ubiquitin/ISG15 ligase TRIM25-like (LOC108702044), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108702044 ( XM_018237052.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018092541.1 Xenopus laevis E3 ubiquitin/ISG15 ligase TRIM25-like (LOC108702044), mRNA.

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CloneID	OAf47274
Clone ID Related Accession (Same CDS sequence)	XM_018237052.1
Accession Version	XM_018237052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	E3 ubiquitin/ISG15 ligase TRIM25-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030740.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG CTGATCTGAG GGATGAACTG ACCTGCTCTG TCTGCCAGGA CATTTACACT 
GATCCTGTAT CCCTGCCCTG TGGCCATAAC TTCTGCCAGG GCTGCATTAG GAGAACATGG 
GAATGGCAGG AGAGTATAGA GGAAGATCCT TCCTGCCCTG AATGCAGACA GAAATACAGG 
AAAAAACCTG AACTACAGAG GAACAGAAGT CTGTGTAACA TAGCAGAGCG ATTCCTTTCT 
ACTCACCCAG AACATAATGG GACTGGGATC TTCTGCACTT ACTGTGTCCA TTCCCATGTA 
CCTGCTGCTA AATCCTGTCT CCTGTGTGAG GCTTCTCTGT GTAATACCCA CGTGAGAGTG 
CACAGCAAGT CAGCAGAACA CGTCTTAATT GAGCCCACCA CTTCCATGGG GAACAGAAAA 
TGCTCTGCAC ACAAAGAGCT CCTAAAGTAT TACTGTTGTG AGGATGGTGC CTGTATCTGT 
GCCTCCTGTT GCCTGGCCGG AGAGCACAGG AGCCACAGGG TGGAGCTGCT GAATGAGGCC 
TCTGAAAAGA AGAAGGAGAA ACTGAGGAAT GTTCTGGAGA AACTGAGCCC AGAGACAGAT 
GAGACTGAGA GAGGAGCCCA GAGACTGCAG GAACACAGGA GAGAAGTGGA AAAAAAAGCA 
GCCAGTGAGA CCGAGAGAGT CACTGTCCTG TTTAGGGACA TTAGGGAACA CCTGGAAACC 
CTAGAGAAGC GACTCCTGAT AAACATCACC AGGCAGAAAG AGGAGCTTTC CCTCCAACTC 
ACCAATCTGA TCCTTGAGCT GGACATAAAG AAGGACGAGT TGTTCAGGAA GATCCATCAA 
ATTGAGGAGC TGTGCAACAT GGCAGATCCA CTCACTGTCC TACAGGAATC AGATGGAGCT 
GTATTTTGTG TGGCTGAGGG GGCAGATAAG GAGGGCAGAG AGAGTGATGA TATAAAGGTT 
CCTGCTGTAG GGGATCTGGA GGTGGATCTG ATCTCAGAGA CATTACTCAC AGGCTTAGCT 
GGGATTGTGA CTGGGGTAAA GGAAAGCATC TCTAGGCACA GTGTTTCTGG TATATTAAAT 
GTTGAAATCC TGCATAGTGA TGATAAAAAC ACATCTATAG AGGGTAAAGA TTCCAAGTAC 
CCCATCTCTT GTAATCAAGC TCCAGACCAT ACTCCTTCCC TTCTGTGTGG ACAGAAGACT 
ACAGACCTGG AACTGGATAT AAATACAGCT GCTAACAATA TATCTGTATC CGAGGACAGG 
AAATCTGCCT TTTACTCACT CCAAGACCAA TGTCGCCCAC AGGCCCCAGA AAGATTTAAA 
TATTATCAAG TCTTAAGCGA AAAAAGTTTT CATTTGGGGC AGCATTACTG GGAAGTGGAG 
GGCAGTAAAA CAGGAGACTG GAGAGTCGGG GTTGCCTATG CCAGTATAGA GAGGGATGGG 
GTACAGTTTC ACCTTGGGGA TAATAGTAAA TCCTGGTGTT TATGCAAAGA GAATAATAAT 
TACACAGTGA GACATGACAA GAAAGAAACC AGTTTACCCC ATGTTTTCTG CAGGAAAATC 
GGAATCTCAC TGGACTATGA GGCCGGGTGT CTGTCCTTTT ATGAGCTGAG TGAGCCTATC 
AGACACTTAC ACACCTTCAC TGCCAAATTC ACTGAGCCCC TTCATGCTGC ATTCTGGGTG 
GGACGCTGGG GTGAAACCTG GGTGACAATC GTTAATTCTT TATTGAATAA CAAAGGTTTA 
ACTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018092541.1
CDS	360..2108
Translation	MAAADLRDELTCSVCQDIYTDPVSLPCGHNFCQGCIRRTWEWQESIEEDPSCPECRQKYRKKPELQRNRSLCNIAERFLSTHPEHNGTGIFCTYCVHSHVPAAKSCLLCEASLCNTHVRVHSKSAEHVLIEPTTSMGNRKCSAHKELLKYYCCEDGACICASCCLAGEHRSHRVELLNEASEKKKEKLRNVLEKLSPETDETERGAQRLQEHRREVEKKAASETERVTVLFRDIREHLETLEKRLLINITRQKEELSLQLTNLILELDIKKDELFRKIHQIEELCNMADPLTVLQESDGAVFCVAEGADKEGRESDDIKVPAVGDLEVDLISETLLTGLAGIVTGVKESISRHSVSGILNVEILHSDDKNTSIEGKDSKYPISCNQAPDHTPSLLCGQKTTDLELDINTAANNISVSEDRKSAFYSLQDQCRPQAPERFKYYQVLSEKSFHLGQHYWEVEGSKTGDWRVGVAYASIERDGVQFHLGDNSKSWCLCKENNNYTVRHDKKETSLPHVFCRKIGISLDYEAGCLSFYELSEPIRHLHTFTAKFTEPLHAAFWVGRWGETWVTIVNSLLNNKGLTT

Target ORF information:

RefSeq Version	XM_018237052.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis E3 ubiquitin/ISG15 ligase TRIM25-like (LOC108702044), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018237052.1

ORF Insert Sequence:

ATGGCGGCTG CTGATCTGAG GGATGAACTG ACCTGCTCTG TCTGCCAGGA CATTTACACT 
GATCCTGTAT CCCTGCCCTG TGGCCATAAC TTCTGCCAGG GCTGCATTAG GAGAACATGG 
GAATGGCAGG AGAGTATAGA GGAAGATCCT TCCTGCCCTG AATGCAGACA GAAATACAGG 
AAAAAACCTG AACTACAGAG GAACAGAAGT CTGTGTAACA TAGCAGAGCG ATTCCTTTCT 
ACTCACCCAG AACATAATGG GACTGGGATC TTCTGCACTT ACTGTGTCCA TTCCCATGTA 
CCTGCTGCTA AATCCTGTCT CCTGTGTGAG GCTTCTCTGT GTAATACCCA CGTGAGAGTG 
CACAGCAAGT CAGCAGAACA CGTCTTAATT GAGCCCACCA CTTCCATGGG GAACAGAAAA 
TGCTCTGCAC ACAAAGAGCT CCTAAAGTAT TACTGTTGTG AGGATGGTGC CTGTATCTGT 
GCCTCCTGTT GCCTGGCCGG AGAGCACAGG AGCCACAGGG TGGAGCTGCT GAATGAGGCC 
TCTGAAAAGA AGAAGGAGAA ACTGAGGAAT GTTCTGGAGA AACTGAGCCC AGAGACAGAT 
GAGACTGAGA GAGGAGCCCA GAGACTGCAG GAACACAGGA GAGAAGTGGA AAAAAAAGCA 
GCCAGTGAGA CCGAGAGAGT CACTGTCCTG TTTAGGGACA TTAGGGAACA CCTGGAAACC 
CTAGAGAAGC GACTCCTGAT AAACATCACC AGGCAGAAAG AGGAGCTTTC CCTCCAACTC 
ACCAATCTGA TCCTTGAGCT GGACATAAAG AAGGACGAGT TGTTCAGGAA GATCCATCAA 
ATTGAGGAGC TGTGCAACAT GGCAGATCCA CTCACTGTCC TACAGGAATC AGATGGAGCT 
GTATTTTGTG TGGCTGAGGG GGCAGATAAG GAGGGCAGAG AGAGTGATGA TATAAAGGTT 
CCTGCTGTAG GGGATCTGGA GGTGGATCTG ATCTCAGAGA CATTACTCAC AGGCTTAGCT 
GGGATTGTGA CTGGGGTAAA GGAAAGCATC TCTAGGCACA GTGTTTCTGG TATATTAAAT 
GTTGAAATCC TGCATAGTGA TGATAAAAAC ACATCTATAG AGGGTAAAGA TTCCAAGTAC 
CCCATCTCTT GTAATCAAGC TCCAGACCAT ACTCCTTCCC TTCTGTGTGG ACAGAAGACT 
ACAGACCTGG AACTGGATAT AAATACAGCT GCTAACAATA TATCTGTATC CGAGGACAGG 
AAATCTGCCT TTTACTCACT CCAAGACCAA TGTCGCCCAC AGGCCCCAGA AAGATTTAAA 
TATTATCAAG TCTTAAGCGA AAAAAGTTTT CATTTGGGGC AGCATTACTG GGAAGTGGAG 
GGCAGTAAAA CAGGAGACTG GAGAGTCGGG GTTGCCTATG CCAGTATAGA GAGGGATGGG 
GTACAGTTTC ACCTTGGGGA TAATAGTAAA TCCTGGTGTT TATGCAAAGA GAATAATAAT 
TACACAGTGA GACATGACAA GAAAGAAACC AGTTTACCCC ATGTTTTCTG CAGGAAAATC 
GGAATCTCAC TGGACTATGA GGCCGGGTGT CTGTCCTTTT ATGAGCTGAG TGAGCCTATC 
AGACACTTAC ACACCTTCAC TGCCAAATTC ACTGAGCCCC TTCATGCTGC ATTCTGGGTG 
GGACGCTGGG GTGAAACCTG GGTGACAATC GTTAATTCTT TATTGAATAA CAAAGGTTTA 
ACTACATAA