mid2.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mid2.L
Entrez Gene ID	100463497
Full Name	midline 2 L homeolog
Synonyms	fxy2,mid2,rnf60,trim1
General protein information	Preferred Names midline 2 L homeolog Names midline 2 L homeolog
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190884.1	NP_001177813.1	midline 2 L homeolog

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

MID1 and MID2 are required for Xenopus neural tube closure through the regulation of microtubule organization.
Suzuki M, Hara Y, Takagi C, Yamamoto TS, Ueno N
Development (Cambridge, England)137(14)2329-39(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mid2.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mid2.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf03895	NM_001190884.1 Latest version!	Xenopus laevis midline 2 L homeolog (mid2.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mid2.L ( NM_001190884.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001177813.1 Xenopus laevis midline 2 L homeolog (mid2.L), mRNA.

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CloneID	OAf03895
Clone ID Related Accession (Same CDS sequence)	NM_001190884.1
Accession Version	NM_001190884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Xenopus laevis(African clawed frog)
Product	midline 2 L homeolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU362930.1. ##Evidence-Data-START## Transcript exon combination :: GU362930.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00012418, SAMD00012419 [ECO:0000350] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGCG CCCATCGTAT CCTCACTTCC 
GGATGTGCTT CCAACGAAAT AGCTGAACCA GTGGCAGTTT TTCAGTGTCC CACTTGCAGA 
TATGTCATAT CTTTAAATCA CCGCGGTCTG GAGGGTTTAA AACGCAACGT TACCTTGCAG 
AACATTATTG ATCGCTTTCA GAAAGCTTCC TTAAGTGTTC CCAACTCTCC CAGTGAAGAT 
CACAGGGAAA AAATCTTTAG GAGTGTCCCC ATTATGGCCT CCGAAAGGAT CTCCTGCCAG 
TTCTGTGAGA CCGAGCCGCC ACGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC 
TACTGTGATC GCTGTCTGAG GGCCACTCAC CCCAATAAAA AGCCCTTCAC TAGCCACAGA 
CTGGTGGAAC CAATTCCAGA CGGTCATTTC CGTGGCCTGG CTTGTCTAGA ACACGAGAAT 
GAAAAGGTCA ACATGTACTG TACTACAGAC GATCAGCTCA TCTGTGCCTT ATGCAAACTG 
GTTGGTAGGC ACAGGGAGCA CCAGGTGGCT TCTTTAACTG ACAGATATGA AAAACTCAAG 
CAAACTTTGG AATCCAATTT TTCCATCTTG GTCAAGCACA GCAGTGAACT CGAAAGCCAG 
ATGACAAAAC TTATTCAGAT TTGCCAGCAG GTCGAGGTAA ATACCAGCAT GCATGAAGCA 
AAACTGAATG AAGAATGTGA TGAGCTAATG GATATCATTC AGAGAAGAAA GCAAATGATA 
GCCGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC 
AATTGCCGTC AACGGCTGGA ACGTTCAACA GTGTTAATCA ACCAAACAGA CCATATACTC 
AAGGAGAGCG ACCATGCAAG GTTTCTGCAG GGAGCGAAAA CTGTAGCAGA GAGAATCTCC 
ATGACAACAA CCTCCTCTCA AATTCCAATG CATGATGTCA ACTTAAACGA CGCCTTTGAG 
AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTTGA CTATGTAACT 
GCTCCCAACC CTCCATCTAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACA 
GTCCACTGGA TGTCGGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTTCA ATACACCATT 
TATACTGGAC AAGCCAACTT CATCAGTCTG TACAGTTCAA TGGACAGTTG GATGATTGTA 
CCTAACATCA AGCAGAACCA TTACACTGTA CATGGGCTCC AGAGCGGCAC ACGGTACATT 
TTCCTGGTCA AAGCCATTAA CCAGGCTGGC AGCAGGCACA GTGATCCTGC AAGACTGAAG 
ACTAACAGCC AGCCCTTTAA GCTTGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC 
AATGACGGAA TGCAGATGGA AAAGGAGGAG AGTTCTCTGA AGAAGAGCCA CACGCCTGAA 
AGGTTCAGCG GAACTGGTTG TTACGGAGCC ACAGGGAACA TCTTCATTGA CAGCGGGTGC 
CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CCATAGGAAT CGCATATAAA 
TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CATGGGTCTT CTCTCGATGC 
AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT CAATCCTCAG 
TTGAAACGCT TAGGTGTTCT CCTAGACTAT GACAATAATA CATTTTCCTT CTATGATCCA 
GCCAATTCCC TCCACCTGCA CACCTTCCAG ATCTCCTTTA TCCTCCCTGT ATGCCCAACC 
TTCACTATAT GGAACAAGTC CATGTTAATA CTCTCAGGAC TCCCTGCTCC AGATTTTATG 
GACTCATTTT TTTCTCAGCC GCAAGAAAGT ATATCTAGAC CACAGGAGTC TCCCTATGTT 
TCTGGGCTTA AGGGTTGCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177813.1
CDS	23..2086
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILTSGCASNEIAEPVAVFQCPTCRYVISLNHRGLEGLKRNVTLQNIIDRFQKASLSVPNSPSEDHREKIFRSVPIMASERISCQFCETEPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPIPDGHFRGLACLEHENEKVNMYCTTDDQLICALCKLVGRHREHQVASLTDRYEKLKQTLESNFSILVKHSSELESQMTKLIQICQQVEVNTSMHEAKLNEECDELMDIIQRRKQMIAVKIKETKVMKLRKLAQQIANCRQRLERSTVLINQTDHILKESDHARFLQGAKTVAERISMTTTSSQIPMHDVNLNDAFENFALDFSREKKLLEGLDYVTAPNPPSIREELCTASHDTITVHWMSDDEFSVGSYELQYTIYTGQANFISLYSSMDSWMIVPNIKQNHYTVHGLQSGTRYIFLVKAINQAGSRHSDPARLKTNSQPFKLDPKYAHKKLKISNDGMQMEKEESSLKKSHTPERFSGTGCYGATGNIFIDSGCHYWEVAVGNSTWYAIGIAYKSAPRNEWVGKNTSSWVFSRCNNNFLVRHNNKEALLDVNPQLKRLGVLLDYDNNTFSFYDPANSLHLHTFQISFILPVCPTFTIWNKSMLILSGLPAPDFMDSFFSQPQESISRPQESPYVSGLKGCQ

Target ORF information:

RefSeq Version	NM_001190884.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis midline 2 L homeolog (mid2.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190884.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGCG CCCATCGTAT CCTCACTTCC 
GGATGTGCTT CCAACGAAAT AGCTGAACCA GTGGCAGTTT TTCAGTGTCC CACTTGCAGA 
TATGTCATAT CTTTAAATCA CCGCGGTCTG GAGGGTTTAA AACGCAACGT TACCTTGCAG 
AACATTATTG ATCGCTTTCA GAAAGCTTCC TTAAGTGTTC CCAACTCTCC CAGTGAAGAT 
CACAGGGAAA AAATCTTTAG GAGTGTCCCC ATTATGGCCT CCGAAAGGAT CTCCTGCCAG 
TTCTGTGAGA CCGAGCCGCC ACGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC 
TACTGTGATC GCTGTCTGAG GGCCACTCAC CCCAATAAAA AGCCCTTCAC TAGCCACAGA 
CTGGTGGAAC CAATTCCAGA CGGTCATTTC CGTGGCCTGG CTTGTCTAGA ACACGAGAAT 
GAAAAGGTCA ACATGTACTG TACTACAGAC GATCAGCTCA TCTGTGCCTT ATGCAAACTG 
GTTGGTAGGC ACAGGGAGCA CCAGGTGGCT TCTTTAACTG ACAGATATGA AAAACTCAAG 
CAAACTTTGG AATCCAATTT TTCCATCTTG GTCAAGCACA GCAGTGAACT CGAAAGCCAG 
ATGACAAAAC TTATTCAGAT TTGCCAGCAG GTCGAGGTAA ATACCAGCAT GCATGAAGCA 
AAACTGAATG AAGAATGTGA TGAGCTAATG GATATCATTC AGAGAAGAAA GCAAATGATA 
GCCGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC 
AATTGCCGTC AACGGCTGGA ACGTTCAACA GTGTTAATCA ACCAAACAGA CCATATACTC 
AAGGAGAGCG ACCATGCAAG GTTTCTGCAG GGAGCGAAAA CTGTAGCAGA GAGAATCTCC 
ATGACAACAA CCTCCTCTCA AATTCCAATG CATGATGTCA ACTTAAACGA CGCCTTTGAG 
AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTTGA CTATGTAACT 
GCTCCCAACC CTCCATCTAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACA 
GTCCACTGGA TGTCGGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTTCA ATACACCATT 
TATACTGGAC AAGCCAACTT CATCAGTCTG TACAGTTCAA TGGACAGTTG GATGATTGTA 
CCTAACATCA AGCAGAACCA TTACACTGTA CATGGGCTCC AGAGCGGCAC ACGGTACATT 
TTCCTGGTCA AAGCCATTAA CCAGGCTGGC AGCAGGCACA GTGATCCTGC AAGACTGAAG 
ACTAACAGCC AGCCCTTTAA GCTTGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC 
AATGACGGAA TGCAGATGGA AAAGGAGGAG AGTTCTCTGA AGAAGAGCCA CACGCCTGAA 
AGGTTCAGCG GAACTGGTTG TTACGGAGCC ACAGGGAACA TCTTCATTGA CAGCGGGTGC 
CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CCATAGGAAT CGCATATAAA 
TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CATGGGTCTT CTCTCGATGC 
AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT CAATCCTCAG 
TTGAAACGCT TAGGTGTTCT CCTAGACTAT GACAATAATA CATTTTCCTT CTATGATCCA 
GCCAATTCCC TCCACCTGCA CACCTTCCAG ATCTCCTTTA TCCTCCCTGT ATGCCCAACC 
TTCACTATAT GGAACAAGTC CATGTTAATA CTCTCAGGAC TCCCTGCTCC AGATTTTATG 
GACTCATTTT TTTCTCAGCC GCAAGAAAGT ATATCTAGAC CACAGGAGTC TCCCTATGTT 
TCTGGGCTTA AGGGTTGCCA GTAA