fgf2 cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	fgf2
Entrez Gene ID	100101279
Full Name	fibroblast growth factor 2 (basic)
Synonyms	FGF-2
General protein information	Preferred Names fibroblast growth factor 2 (basic) Names fibroblast growth factor 2 BFGF HBGF-2 basic fibroblast growth factor heparin-binding growth factor 2
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099871.1	NP_001093341.1	fibroblast growth factor 2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Axonal filopodial asymmetry induced by synaptic target.
Li PP, Chen C, Lee CW, Madhavan R, Peng HB
Molecular biology of the cell22(14)2480-90(2011 Jul)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

The presence of fibroblast growth factor in the frog egg: its role as a natural mesoderm inducer.
Kimelman D, Abraham JA, Haaparanta T, Palisi TM, Kirschner MW
Science (New York, N.Y.)242(4881)1053-6(1988 Nov)

Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
Kimelman D, Kirschner M
Cell51(5)869-77(1987 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that bFGF activation of neuronal FGFR1 generates filopodial processes in neurons that promote nerve-muscle interaction and facilitate NMJ establishment.
Title: Axonal filopodial asymmetry induced by synaptic target.

The following fgf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fgf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf03200	NM_001099871.1 Latest version!	Xenopus laevis fibroblast growth factor 2 L homeolog (fgf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fgf2 ( NM_001099871.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001093341.1 Xenopus laevis fibroblast growth factor 2 L homeolog (fgf2), mRNA.

Price & Availability↑

CloneID	OAf03200
Clone ID Related Accession (Same CDS sequence)	NM_001099871.1
Accession Version	NM_001099871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Xenopus laevis(African clawed frog)
Product	fibroblast growth factor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M18067.1. ##Evidence-Data-START## Transcript exon combination :: M18067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012419, SAMN04157067 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCAG GGAGCATCAC AACTCTGCCA ACTGAATCCG AGGATGGGGG AAACACTCCT 
TTTTCACCAG GGAGTTTTAA AGACCCCAAG AGGCTCTACT GCAAGAACGG GGGCTTCTTC 
CTCAGGATAA ACTCAGACGG GAGAGTGGAC GGGTCAAGGG ACAAAAGTGA CTCGCACATA 
AAATTACAGC TACAAGCTGT AGAGCGGGGA GTGGTATCAA TAAAGGGAAT CACTGCAAAT 
CGCTACCTTG CCATGAAGGA AGATGGGAGA TTAACATCGC TGAGGTGTAT AACAGATGAA 
TGCTTCTTTT TTGAACGACT GGAAGCTAAT AACTACAACA CTTACCGGTC TCGGAAATAC 
AGCAGCTGGT ATGTGGCACT AAAGCGAACC GGGCAGTACA AAAATGGATC GAGCACTGGA 
CCGGGACAAA AAGCTATTTT ATTTCTCCCA ATGTCCGCAA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093341.1
CDS	335..802
Translation	MAAGSITTLPTESEDGGNTPFSPGSFKDPKRLYCKNGGFFLRINSDGRVDGSRDKSDSHIKLQLQAVERGVVSIKGITANRYLAMKEDGRLTSLRCITDECFFFERLEANNYNTYRSRKYSSWYVALKRTGQYKNGSSTGPGQKAILFLPMSAKS

Target ORF information:

RefSeq Version	NM_001099871.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis fibroblast growth factor 2 L homeolog (fgf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099871.1

ORF Insert Sequence:

ATGGCGGCAG GGAGCATCAC AACTCTGCCA ACTGAATCCG AGGATGGGGG AAACACTCCT 
TTTTCACCAG GGAGTTTTAA AGACCCCAAG AGGCTCTACT GCAAGAACGG GGGCTTCTTC 
CTCAGGATAA ACTCAGACGG GAGAGTGGAC GGGTCAAGGG ACAAAAGTGA CTCGCACATA 
AAATTACAGC TACAAGCTGT AGAGCGGGGA GTGGTATCAA TAAAGGGAAT CACTGCAAAT 
CGCTACCTTG CCATGAAGGA AGATGGGAGA TTAACATCGC TGAGGTGTAT AACAGATGAA 
TGCTTCTTTT TTGAACGACT GGAAGCTAAT AACTACAACA CTTACCGGTC TCGGAAATAC 
AGCAGCTGGT ATGTGGCACT AAAGCGAACC GGGCAGTACA AAAATGGATC GAGCACTGGA 
CCGGGACAAA AAGCTATTTT ATTTCTCCCA ATGTCCGCAA AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Axonal filopodial asymmetry induced by synaptic target.
Molecular biology of the cell22(14)2480-90(2011 Jul)
Li PP,Chen C,Lee CW,Madhavan R,Peng HB

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

The presence of fibroblast growth factor in the frog egg: its role as a natural mesoderm inducer.
Science (New York, N.Y.)242(4881)1053-6(1988 Nov)
Kimelman D,Abraham JA,Haaparanta T,Palisi TM,Kirschner MW

Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
Cell51(5)869-77(1987 Dec)
Kimelman D,Kirschner M