BN7_5487 cDNA ORF clone, Wickerhamomyces ciferrii

Gene
Clones
gRNAs

Summary

Gene Symbol	BN7_5487
Entrez Gene ID	23468430
Full Name	E3 ubiquitin-protein ligase
Gene Type	protein-coding
Organism	Wickerhamomyces ciferrii

mRNA and Protein(s)

mRNA	Protein	Name
XM_011273913.1	XP_011272215.1	E3 ubiquitin-protein ligase

Pathways from BioSystems
Gene Ontology

The following BN7_5487 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BN7_5487 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OWi06733	XM_011273913.1 Latest version!	Wickerhamomyces ciferrii E3 ubiquitin-protein ligase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BN7_5487 ( XM_011273913.1 ) cDNA ORF clone, Wickerhamomyces ciferrii -> XP_011272215.1 Wickerhamomyces ciferrii E3 ubiquitin-protein ligase partial mRNA.

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CloneID	OWi06733
Clone ID Related Accession (Same CDS sequence)	XM_011273913.1
Accession Version	XM_011273913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-09
Organism	Wickerhamomyces ciferrii
Product	E3 ubiquitin-protein ligase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011887523). COMPLETENESS: incomplete on both ends.

ATGAAGCTTT TCAAAAGTCC AAGACTAAAT TCCTGTCTCA TACTAAGTAT ATGCTTATTA 
TTGCAAATTT CTAATACATC ACCAGTTCCT CAAGGTCATG TCTCAGGCTT TTCAAATCCT 
GATGAAGTTC AAGTAAATTA CCCAGATGAA CTCTCAACTG ATCATAACAT AACAGAAATC 
ATGGATATCA ACAAATCAAA CACATCGGAG GCTCATGGTG ATGATGAATA TTCTTTATTT 
AAATCAGGAT CAAATAATGA TAAAACTGAG ATTGAATTAG AAGCCGACAA TTCTGAAAAA 
GAGCATAAAA ATGAATTCGA TTTAGTTCAT CTATGTTGGA TTATTCCAAT TGGTTTGTTG 
TTTATGATAG GTTCATTAGG ATTACTTTGT GGTAATTACA CGTCGGTATA TCTCATTTAT 
CACTTGATAG TTGGTACATA CAAATTATTA AGACTAAATG AAGATGAGTA TCCACCAATA 
CAAGAGCCAG AAACTGAACT CCCAGTTTCC AATGATAGGG ATCCAAATTC AAACGTTGTA 
ACAAGATCAC AAATTGATTC CCATATTTAT GACCAAAACA AACTAGAAGA AGTTTCCAAA 
TCCTCAATTA TACCACCACA TGATTCTTAT AAACTTATTA CTACAGATCA ATTAGAAACA 
CCTGAATATG AAGAGTGGAG AAACCAAGAT GTTTGTTTGA TCTGTCTTGA ATCATTTAAT 
GAGATCCGAG ATCATTTAGC TCAACTACCT TGTGCACATA CATTCCATTA TTCATGTTTT 
ATAAAGACAG GCAGTATAAA TGGGGATAGT AATAATGTAC TTGGGAGTCC AAATATGAGA 
TGTTGTGCTT GTCAATTAAG TTTGATTAAA TATCATCAGT ATCTTGCTGA TTATAAACTA 
GATCATAAAC AAGTTGAATT TGTTAATAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011272215.1
CDS	1..933
Translation	MKLFKSPRLNSCLILSICLLLQISNTSPVPQGHVSGFSNPDEVQVNYPDELSTDHNITEIMDINKSNTSEAHGDDEYSLFKSGSNNDKTEIELEADNSEKEHKNEFDLVHLCWIIPIGLLFMIGSLGLLCGNYTSVYLIYHLIVGTYKLLRLNEDEYPPIQEPETELPVSNDRDPNSNVVTRSQIDSHIYDQNKLEEVSKSSIIPPHDSYKLITTDQLETPEYEEWRNQDVCLICLESFNEIRDHLAQLPCAHTFHYSCFIKTGSINGDSNNVLGSPNMRCCACQLSLIKYHQYLADYKLDHKQVEFVNK

Target ORF information:

RefSeq Version	XM_011273913.1
Organism	Wickerhamomyces ciferrii
Definition	Wickerhamomyces ciferrii E3 ubiquitin-protein ligase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011273913.1

ORF Insert Sequence:

ATGAAGCTTT TCAAAAGTCC AAGACTAAAT TCCTGTCTCA TACTAAGTAT ATGCTTATTA 
TTGCAAATTT CTAATACATC ACCAGTTCCT CAAGGTCATG TCTCAGGCTT TTCAAATCCT 
GATGAAGTTC AAGTAAATTA CCCAGATGAA CTCTCAACTG ATCATAACAT AACAGAAATC 
ATGGATATCA ACAAATCAAA CACATCGGAG GCTCATGGTG ATGATGAATA TTCTTTATTT 
AAATCAGGAT CAAATAATGA TAAAACTGAG ATTGAATTAG AAGCCGACAA TTCTGAAAAA 
GAGCATAAAA ATGAATTCGA TTTAGTTCAT CTATGTTGGA TTATTCCAAT TGGTTTGTTG 
TTTATGATAG GTTCATTAGG ATTACTTTGT GGTAATTACA CGTCGGTATA TCTCATTTAT 
CACTTGATAG TTGGTACATA CAAATTATTA AGACTAAATG AAGATGAGTA TCCACCAATA 
CAAGAGCCAG AAACTGAACT CCCAGTTTCC AATGATAGGG ATCCAAATTC AAACGTTGTA 
ACAAGATCAC AAATTGATTC CCATATTTAT GACCAAAACA AACTAGAAGA AGTTTCCAAA 
TCCTCAATTA TACCACCACA TGATTCTTAT AAACTTATTA CTACAGATCA ATTAGAAACA 
CCTGAATATG AAGAGTGGAG AAACCAAGAT GTTTGTTTGA TCTGTCTTGA ATCATTTAAT 
GAGATCCGAG ATCATTTAGC TCAACTACCT TGTGCACATA CATTCCATTA TTCATGTTTT 
ATAAAGACAG GCAGTATAAA TGGGGATAGT AATAATGTAC TTGGGAGTCC AAATATGAGA 
TGTTGTGCTT GTCAATTAAG TTTGATTAAA TATCATCAGT ATCTTGCTGA TTATAAACTA 
GATCATAAAC AAGTTGAATT TGTTAATAAG TGA