MCF2 cDNA ORF clone, Tyto alba(Barn owl)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCF2
Entrez Gene ID	104357690
Full Name	MCF.2 cell line derived transforming sequence
Gene Type	protein-coding
Organism	Tyto alba(Barn owl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009963152.1	XP_009961454.1	proto-oncogene DBL

Pathways from BioSystems
Gene Ontology

The following MCF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTy02051	XM_009963152.1 Latest version!	Tyto alba MCF.2 cell line derived transforming sequence (MCF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCF2 ( XM_009963152.1 ) cDNA ORF clone, Tyto alba(Barn owl) -> XP_009961454.1 Tyto alba MCF.2 cell line derived transforming sequence (MCF2), mRNA.

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CloneID	OTy02051
Clone ID Related Accession (Same CDS sequence)	XM_009963152.1
Accession Version	XM_009963152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Tyto alba(Barn owl)
Product	proto-oncogene DBL
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009986017.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tyto alba Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGAGTTTGC CCCTGCGGCG GATGAGGCAG GAGGATGCGG GTGCCCGGGT ATGGTCTCTT 
CTTGTGTGGT GTGTGGAACA CGTTGGCATC CTGCAGTCAC GAAACCAAAG GTGTCCAGAG 
GCGCCTTTGC TCTCAGAGAG GCGTTTCACA CCTCCTCGCT ACTGCGGTGC GTCTTTCCCG 
GGGAACCTGC ACTTAGTGAT GGTTCTTCGT CCCACGGGGT TTTTCCAGCG CACCTTCACT 
GACATTGGAT TTCGGTTCAG TCAGGAGGAT TTTCTACTCA AGTTACCGGT TGTTATGCTG 
AGCTCAGTTA GCGACCTGCT GACGTACATC GATGAGCAGC AGTTAACCCC GGAGTTTGGA 
GGCACCCTGG AGTACTGCCA CAGCGAGTGG GTCATCTTCA GGACTGCAAT AGAGAGTTTT 
GCACTAACCG TGAAGGAAAT TGCACAGATG TTACAATCCT TTGGCACGGA GCTGGCAGAG 
ACTGAGCTAC CGGATGACAT GTATTCAATT GAGCGCATCC TGGCCCTGCG CACGGAAAAG 
TACTACCAGC TCAAGGAAGA TATTACAGCA GTCACAAAAG AAGGAAACTT GCTTTTGAGC 
AGTTTTGAAG AGCCTGACTC CGGGGAATGC AGTCAGGACC AGCACCACGA GAGGCCTGGG 
GACTGGGAGA CCGTGAATCG GTTACTTGGT CAGCTTCACG AGATGGAGAC TGCTTTTGAT 
GGCTTCTGGG AAAAACATCA GTTAAAAATG GAGCAATATT TGCAACTGTG GAAGTTTGAG 
CAAAGTTTTC AAGAGGTCAA GAATGCCATT GAATTTTTAA TGGGTCAGCA AGCAGAGCTG 
CCCGACACTG GCGACAGCGT CCCCCAAGTA AAACAGAGGC TCAAGGACTT GGGTCATTTC 
GATGGTATGG CTCAGGACCT GATAGGGAAG GCTCAGGTGG TGATACTACA CGGACACCAG 
CTAGCAGCAA ATCATCACTA CGCACTCAAC TTAATCTGCC AGCAATGCAA CGAGCTGCGG 
CACCATTCTG ATGTCTTGTC TGATGAGATC AAAAGGAAGC AGATGCGACT GCAGAAGACC 
TTGGATCTTC ATACCCGCCT TCAGCAGGCT CTGCAGTGCT GTGATGAAGG TGCCTACCTG 
CTTGCCAACC AGCAGATGGA CAAGTGCCAG TCTAAAGAAG GCGCTCAGAA AGCCCTCCAG 
GACATAGAAA GATTTCTTGA AAGCTCTTCA GCCTACTTAA ACTATGATCC CCAAGCCCTG 
CACTACGATT TTGAGTCAAT CTTAACATCT GAACTGAAGT GCCAGATACA GTCAATGCAG 
GTCAAGCTTG AAAACGTTCG AAGCATGTTT GAGAATCGGC AGTCTTGCTT CCGGAAGCTG 
TTGGATAAAC ACGTGCGGCC TGTCCAATTG GTAGCCCCTC GGCCAGAAAA TCCACCGAGA 
TCAAAATCAC CATTATTCTC TCCAAAACAT GGTGTGGATT TTAATTCGAG TTTGAAATTT 
TCATTTGATC TCTCCCTCCC TGGGAAGAAA ACATCAAGAA AAACTCCAAG TTCTCGCAAG 
ATTGAAGTAA TGCACGACTA TCAGGAAAAA CGGAATTCCT TGCAATATTT TATTTCAGAC 
AGCGAGGACA GCTTAGATAT ACTAAAAGGC CATGTCATAA ATGAACTTAT AGAAACTGAG 
AGAGTGTACG TAGAGGAACT GTTCACTGTT TTGACGGGCT ACAGAGCTGA GATGGATAAC 
CCTGCCATGC TCCTCCTCAT GCCTCCCGTG CTGAGGAACA GGAAGGACGT CCTCTTTGGA 
AACATGCCCG AGATATACGA CTTCCACAAC AAAATTTTCC TGCACAGTTT GGAAAGTTGC 
CTGGGAGCAC CCGAGAGAGT GGGATTTTGT TTCCTAGACA GGCGGGAGGA TTTCCAGATG 
TATGAGAAAT ACTGCCAGAA CAAGCCCCGG TCGGAGTCCC TGTGGAGGCA GTGCTCCGAA 
AGCACCTTCT TCCAGCACAA ACTCGGGCTG GATTCCTATT TGCTCAAACC AGTGCAGCGC 
CTGACAAAAT ACCAGTTGCT TCTGAAGGAG TTGCTAAAGT ACAGCACAAG CTGTGATGGA 
GTTCAGGAAC TTCAAGAAGC TCTGGTCGCA ATGTTGGATT TGCTAAAGTC AGTCAATGAC 
TCTATGCATC AGATTTCAAT AACAGGATAT GATGGTGACC TGAGCGAACT GGGGAAGGTA 
TTGATGCAAG GATCTTTCAG TGTTTGGACA GGACACCGAA AAGGTCCAAC AAAAATGAAG 
GATCTTGCCA GGTTCAAGCC AATGCAGAGG CATCTGTTCC TGTATGAGAA AGCCTTGGTC 
TTCTGCAAGA AGCGGGAGGA GCATGGAGAT GGATATGACA AAACCTCATC TTACAGTTTC 
AAGCATTTTT TGAAAATGAA TGCAGTTGGA ATAACTGAGA ATGTGAAAGG AGATCATCGG 
AAATTTGAAA TCTGGTATAG CGGGCGAGAA GAAGTCTATG TCGTGCAGGC CCAAACAGTA 
GATCTGAAGA TGGCATGGTT GAATGAAATA AGAAAAATTT TGTTCAAGCA GCAGGAACTT 
ATAAAAGTGG AGAAGCAGCA GCCTGGCTCA TGCACGGACC AGCTCCCGCT CTCCTCCCAG 
CTGAGTGATG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009961454.1
CDS	1..2655
Translation	MSLPLRRMRQEDAGARVWSLLVWCVEHVGILQSRNQRCPEAPLLSERRFTPPRYCGASFPGNLHLVMVLRPTGFFQRTFTDIGFRFSQEDFLLKLPVVMLSSVSDLLTYIDEQQLTPEFGGTLEYCHSEWVIFRTAIESFALTVKEIAQMLQSFGTELAETELPDDMYSIERILALRTEKYYQLKEDITAVTKEGNLLLSSFEEPDSGECSQDQHHERPGDWETVNRLLGQLHEMETAFDGFWEKHQLKMEQYLQLWKFEQSFQEVKNAIEFLMGQQAELPDTGDSVPQVKQRLKDLGHFDGMAQDLIGKAQVVILHGHQLAANHHYALNLICQQCNELRHHSDVLSDEIKRKQMRLQKTLDLHTRLQQALQCCDEGAYLLANQQMDKCQSKEGAQKALQDIERFLESSSAYLNYDPQALHYDFESILTSELKCQIQSMQVKLENVRSMFENRQSCFRKLLDKHVRPVQLVAPRPENPPRSKSPLFSPKHGVDFNSSLKFSFDLSLPGKKTSRKTPSSRKIEVMHDYQEKRNSLQYFISDSEDSLDILKGHVINELIETERVYVEELFTVLTGYRAEMDNPAMLLLMPPVLRNRKDVLFGNMPEIYDFHNKIFLHSLESCLGAPERVGFCFLDRREDFQMYEKYCQNKPRSESLWRQCSESTFFQHKLGLDSYLLKPVQRLTKYQLLLKELLKYSTSCDGVQELQEALVAMLDLLKSVNDSMHQISITGYDGDLSELGKVLMQGSFSVWTGHRKGPTKMKDLARFKPMQRHLFLYEKALVFCKKREEHGDGYDKTSSYSFKHFLKMNAVGITENVKGDHRKFEIWYSGREEVYVVQAQTVDLKMAWLNEIRKILFKQQELIKVEKQQPGSCTDQLPLSSQLSDG

Target ORF information:

RefSeq Version	XM_009963152.1
Organism	Tyto alba(Barn owl)
Definition	Tyto alba MCF.2 cell line derived transforming sequence (MCF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009963152.1

ORF Insert Sequence:

ATGAGTTTGC CCCTGCGGCG GATGAGGCAG GAGGATGCGG GTGCCCGGGT ATGGTCTCTT 
CTTGTGTGGT GTGTGGAACA CGTTGGCATC CTGCAGTCAC GAAACCAAAG GTGTCCAGAG 
GCGCCTTTGC TCTCAGAGAG GCGTTTCACA CCTCCTCGCT ACTGCGGTGC GTCTTTCCCG 
GGGAACCTGC ACTTAGTGAT GGTTCTTCGT CCCACGGGGT TTTTCCAGCG CACCTTCACT 
GACATTGGAT TTCGGTTCAG TCAGGAGGAT TTTCTACTCA AGTTACCGGT TGTTATGCTG 
AGCTCAGTTA GCGACCTGCT GACGTACATC GATGAGCAGC AGTTAACCCC GGAGTTTGGA 
GGCACCCTGG AGTACTGCCA CAGCGAGTGG GTCATCTTCA GGACTGCAAT AGAGAGTTTT 
GCACTAACCG TGAAGGAAAT TGCACAGATG TTACAATCCT TTGGCACGGA GCTGGCAGAG 
ACTGAGCTAC CGGATGACAT GTATTCAATT GAGCGCATCC TGGCCCTGCG CACGGAAAAG 
TACTACCAGC TCAAGGAAGA TATTACAGCA GTCACAAAAG AAGGAAACTT GCTTTTGAGC 
AGTTTTGAAG AGCCTGACTC CGGGGAATGC AGTCAGGACC AGCACCACGA GAGGCCTGGG 
GACTGGGAGA CCGTGAATCG GTTACTTGGT CAGCTTCACG AGATGGAGAC TGCTTTTGAT 
GGCTTCTGGG AAAAACATCA GTTAAAAATG GAGCAATATT TGCAACTGTG GAAGTTTGAG 
CAAAGTTTTC AAGAGGTCAA GAATGCCATT GAATTTTTAA TGGGTCAGCA AGCAGAGCTG 
CCCGACACTG GCGACAGCGT CCCCCAAGTA AAACAGAGGC TCAAGGACTT GGGTCATTTC 
GATGGTATGG CTCAGGACCT GATAGGGAAG GCTCAGGTGG TGATACTACA CGGACACCAG 
CTAGCAGCAA ATCATCACTA CGCACTCAAC TTAATCTGCC AGCAATGCAA CGAGCTGCGG 
CACCATTCTG ATGTCTTGTC TGATGAGATC AAAAGGAAGC AGATGCGACT GCAGAAGACC 
TTGGATCTTC ATACCCGCCT TCAGCAGGCT CTGCAGTGCT GTGATGAAGG TGCCTACCTG 
CTTGCCAACC AGCAGATGGA CAAGTGCCAG TCTAAAGAAG GCGCTCAGAA AGCCCTCCAG 
GACATAGAAA GATTTCTTGA AAGCTCTTCA GCCTACTTAA ACTATGATCC CCAAGCCCTG 
CACTACGATT TTGAGTCAAT CTTAACATCT GAACTGAAGT GCCAGATACA GTCAATGCAG 
GTCAAGCTTG AAAACGTTCG AAGCATGTTT GAGAATCGGC AGTCTTGCTT CCGGAAGCTG 
TTGGATAAAC ACGTGCGGCC TGTCCAATTG GTAGCCCCTC GGCCAGAAAA TCCACCGAGA 
TCAAAATCAC CATTATTCTC TCCAAAACAT GGTGTGGATT TTAATTCGAG TTTGAAATTT 
TCATTTGATC TCTCCCTCCC TGGGAAGAAA ACATCAAGAA AAACTCCAAG TTCTCGCAAG 
ATTGAAGTAA TGCACGACTA TCAGGAAAAA CGGAATTCCT TGCAATATTT TATTTCAGAC 
AGCGAGGACA GCTTAGATAT ACTAAAAGGC CATGTCATAA ATGAACTTAT AGAAACTGAG 
AGAGTGTACG TAGAGGAACT GTTCACTGTT TTGACGGGCT ACAGAGCTGA GATGGATAAC 
CCTGCCATGC TCCTCCTCAT GCCTCCCGTG CTGAGGAACA GGAAGGACGT CCTCTTTGGA 
AACATGCCCG AGATATACGA CTTCCACAAC AAAATTTTCC TGCACAGTTT GGAAAGTTGC 
CTGGGAGCAC CCGAGAGAGT GGGATTTTGT TTCCTAGACA GGCGGGAGGA TTTCCAGATG 
TATGAGAAAT ACTGCCAGAA CAAGCCCCGG TCGGAGTCCC TGTGGAGGCA GTGCTCCGAA 
AGCACCTTCT TCCAGCACAA ACTCGGGCTG GATTCCTATT TGCTCAAACC AGTGCAGCGC 
CTGACAAAAT ACCAGTTGCT TCTGAAGGAG TTGCTAAAGT ACAGCACAAG CTGTGATGGA 
GTTCAGGAAC TTCAAGAAGC TCTGGTCGCA ATGTTGGATT TGCTAAAGTC AGTCAATGAC 
TCTATGCATC AGATTTCAAT AACAGGATAT GATGGTGACC TGAGCGAACT GGGGAAGGTA 
TTGATGCAAG GATCTTTCAG TGTTTGGACA GGACACCGAA AAGGTCCAAC AAAAATGAAG 
GATCTTGCCA GGTTCAAGCC AATGCAGAGG CATCTGTTCC TGTATGAGAA AGCCTTGGTC 
TTCTGCAAGA AGCGGGAGGA GCATGGAGAT GGATATGACA AAACCTCATC TTACAGTTTC 
AAGCATTTTT TGAAAATGAA TGCAGTTGGA ATAACTGAGA ATGTGAAAGG AGATCATCGG 
AAATTTGAAA TCTGGTATAG CGGGCGAGAA GAAGTCTATG TCGTGCAGGC CCAAACAGTA 
GATCTGAAGA TGGCATGGTT GAATGAAATA AGAAAAATTT TGTTCAAGCA GCAGGAACTT 
ATAAAAGTGG AGAAGCAGCA GCCTGGCTCA TGCACGGACC AGCTCCCGCT CTCCTCCCAG 
CTGAGTGATG GGTGA