LOC101350772 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101350772
Entrez Gene ID	101350772
Full Name	inositol polyphosphate-5-phosphatase D
Synonyms	INPP5D
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004389480.2	XP_004389537.1	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
XM_023724505.1	XP_023580273.1	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X6
XM_023724503.1	XP_023580271.1	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X3
XM_023724504.1	XP_023580272.1	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X4
XM_023724500.1	XP_023580268.1	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X1
XM_023724502.1	XP_023580270.1	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X2
XM_004389480.3	XP_004389537.1	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X5

Pathways from BioSystems
Gene Ontology

The following LOC101350772 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101350772 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr187906	XM_004389480.3 Latest version!	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTr227348	XM_023724500.1 Latest version!	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTr227349	XM_023724502.1 Latest version!	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTr227350	XM_023724503.1 Latest version!	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTr227351	XM_023724504.1 Latest version!	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTr227352	XM_023724505.1 Latest version!	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OTr187906	XM_004389480.2	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase, 145kDa (LOC101350772), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101350772 ( XM_004389480.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004389537.1 Trichechus manatus latirostris inositol polyphosphate-5-phosphatase, 145kDa (LOC101350772), mRNA.

Price & Availability↑

CloneID	OTr187906
Clone ID Related Accession (Same CDS sequence)	XM_004389480.2 , XM_004389480.3
Accession Version	XM_004389480.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444185.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004389480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCT GCTGGAACCA TGGCAATATC ACGCGCTCTA AGGCCGAGGA GCTGCTCTCC 
AGGACGGGCA AGGATGGGAG CTTTCTCGTG CGTGCCAGTG AGTCCATCTC CCGGGCTTAC 
GCCCTCTGCG TGCTGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004389537.1
CDS	124..3699
Translation	MVPCWNHGNITRSKAEELLSRTGKDGSFLVRASESISRAYALCVLYRNCVYTYRILPNEDKFSVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQYPVPLEEEDASDEPEEETECDVSPPELPPRNIPLSAGPCEAKEVPLSSESPRVPEVSKPTLSETLLQRLQSIDTSGLPDEYLKAIQDYLSTQLVLDSEFVKTGSSSLPHLKKLTTLLCKELYGEVVRTLPTLEFLQRLFDQQLSPGLRPRPQVSGEANPINMVAKFSQLTSLLSSIEDKVKAMLHEGHESPHRCSIIPPVNFEVKSESLGIPQKLHLKVDVETGKLIVKKSKDGSEDKYYSHKKILQLVKSQKFLNKLVIVVETEKEKTVRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILKHSLQEITSMTFKTMAIHTLWNIRIVVLAKPEHENRISHVCTNHVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYTNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRLELPISEADTIIQKIKQQQYADLLAHDQLLMERKEQKVFLHFDEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLSCYATLKTKSQTKFYLEFHSSCLESFVKSQEGENEEGSEGELLVKFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLEAKETQFPIYTPLTHHGEMMGHFQGEIKLQTSEGKTREKLYDFVKTERDESSGPKSLKSFTSHDPMKLWEPTNRAPLCSGPSITEIINPNYMGVGPFGPPMPLHGKQVISPDQQPTAWSYDPLPKDASLGPGWGESPPTPPSQQPVSPKKFSPSPASRGPSPRTQESRPSDLAKNIAEPLLQEDLPLTKQKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPDPGVSSPTILLTKAQEAESSKGTSKQVPTPRLRSFTCSSSTAEAGAAAGGEKNHGKPKPAASSQAPAPVPAKRPVKPSRSEMSQPIPPAPTQLRPPLPAKSLAVLHLQHSKGRDYRDNTELLHHGKHRPEEGPLGRTAMQ

Target ORF information:

RefSeq Version	XM_004389480.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase, 145kDa (LOC101350772), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004389480.2

ORF Insert Sequence:

ATGGTGCCCT GCTGGAACCA TGGCAATATC ACGCGCTCTA AGGCCGAGGA GCTGCTCTCC 
AGGACGGGCA AGGATGGGAG CTTTCTCGTG CGTGCCAGTG AGTCCATCTC CCGGGCTTAC 
GCCCTCTGCG TGCTGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101350772 ( XM_004389480.3 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004389537.1 Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTr187906
Clone ID Related Accession (Same CDS sequence)	XM_004389480.2 , XM_004389480.3
Accession Version	XM_004389480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444185.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004389480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCT GCTGGAACCA TGGCAATATC ACGCGCTCTA AGGCCGAGGA GCTGCTCTCC 
AGGACGGGCA AGGATGGGAG CTTTCTCGTG CGTGCCAGTG AGTCCATCTC CCGGGCTTAC 
GCCCTCTGCG TGCTGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004389537.1
CDS	132..3707
Translation	MVPCWNHGNITRSKAEELLSRTGKDGSFLVRASESISRAYALCVLYRNCVYTYRILPNEDKFSVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQYPVPLEEEDASDEPEEETECDVSPPELPPRNIPLSAGPCEAKEVPLSSESPRVPEVSKPTLSETLLQRLQSIDTSGLPDEYLKAIQDYLSTQLVLDSEFVKTGSSSLPHLKKLTTLLCKELYGEVVRTLPTLEFLQRLFDQQLSPGLRPRPQVSGEANPINMVAKFSQLTSLLSSIEDKVKAMLHEGHESPHRCSIIPPVNFEVKSESLGIPQKLHLKVDVETGKLIVKKSKDGSEDKYYSHKKILQLVKSQKFLNKLVIVVETEKEKTVRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILKHSLQEITSMTFKTMAIHTLWNIRIVVLAKPEHENRISHVCTNHVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYTNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRLELPISEADTIIQKIKQQQYADLLAHDQLLMERKEQKVFLHFDEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLSCYATLKTKSQTKFYLEFHSSCLESFVKSQEGENEEGSEGELLVKFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLEAKETQFPIYTPLTHHGEMMGHFQGEIKLQTSEGKTREKLYDFVKTERDESSGPKSLKSFTSHDPMKLWEPTNRAPLCSGPSITEIINPNYMGVGPFGPPMPLHGKQVISPDQQPTAWSYDPLPKDASLGPGWGESPPTPPSQQPVSPKKFSPSPASRGPSPRTQESRPSDLAKNIAEPLLQEDLPLTKQKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPDPGVSSPTILLTKAQEAESSKGTSKQVPTPRLRSFTCSSSTAEAGAAAGGEKNHGKPKPAASSQAPAPVPAKRPVKPSRSEMSQPIPPAPTQLRPPLPAKSLAVLHLQHSKGRDYRDNTELLHHGKHRPEEGPLGRTAMQ

Target ORF information:

RefSeq Version	XM_004389480.3
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004389480.3

ORF Insert Sequence:

ATGGTGCCCT GCTGGAACCA TGGCAATATC ACGCGCTCTA AGGCCGAGGA GCTGCTCTCC 
AGGACGGGCA AGGATGGGAG CTTTCTCGTG CGTGCCAGTG AGTCCATCTC CCGGGCTTAC 
GCCCTCTGCG TGCTGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101350772 ( XM_023724500.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023580268.1 Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X1, mRNA.

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CloneID	OTr227348
Clone ID Related Accession (Same CDS sequence)	XM_023724500.1
Accession Version	XM_023724500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444185.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGGTAG GCGGGGAAGG 
CAGGAGGCCG GGCAGCTGGC TCAGCAGGGA GGTGCCTTAG GGACCACGTC CTCCAAGGAA 
CATGGTGGAG AAGTGCTGGA TGTTTCTCTC CCCTCGAATG CTTGCATAGA AGAATGCGAC 
GTATCTCCAC CTGAGCTGCC CCCCAGGAAC ATCCCTTTGT CTGCTGGGCC CTGTGAAGCC 
AAGGAAGTTC CTCTTTCAAG CGAGAGTCCC CGAGTGCCCG AGGTCAGCAA GCCGACCCTC 
TCTGAGACGT TACTTCAGAG GCTGCAGAGC ATCGACACTA GTGGGCTTCC GGACGAGTAT 
CTTAAAGCCA TCCAGGATTA TTTAAGCACG CAGCTAGTCC TCGACTCTGA GTTTGTGAAG 
ACGGGCTCCA GCAGTCTCCC TCACCTGAAG AAACTGACCA CGCTCCTGTG CAAGGAGCTT 
TATGGCGAGG TCGTTCGGAC TCTTCCCACT CTGGAGTTTC TGCAGAGGCT GTTCGACCAG 
CAGCTGTCCC CTGGCCTCCG CCCACGTCCT CAGGTGTCTG GTGAGGCCAA TCCCATCAAC 
ATGGTGGCCA AGTTCAGCCA ACTGACAAGT CTGCTGTCCT CTATTGAAGA TAAGGTCAAG 
GCCATGCTGC ATGAGGGTCA TGAGTCTCCT CACCGGTGCT CCATCATCCC TCCGGTCAAC 
TTCGAGGTAA AGTCAGAGTC CTTGGGGATT CCTCAGAAAT TGCATCTCAA GGTCGACGTT 
GAGACTGGGA AACTGATCGT TAAGAAGTCC AAGGATGGTT CTGAGGACAA GTACTACAGC 
CACAAGAAAA TCCTGCAGCT CGTCAAGTCT CAGAAGTTTC TTAACAAGCT GGTGATTGTG 
GTGGAAACGG AGAAGGAGAA GACCGTGCGG AAGGAATATG TCTTTGCTGA CTCTAAAAAG 
AGAGAAGGCT TCTGCCAGCT TCTCCAGCAG ATGAAGAACA AGCACTCGGA GCAGCCTGAG 
CCCGACATGA TCACCATCTT CATCGGCACC TGGAACATGG GTAACGCCCC CCCTCCCAAG 
AAGATCACGT CCTGGTTTCT CTCCAAGGGG CAGGGAAAGA CGCGGGATGA CTCAGCAGAC 
TACATCCCCC ATGACATCTA TGTGATCGGC ACCCAGGAGG ACCCCCTGGG AGAAAAGGAG 
TGGCTGGAGA TCCTCAAGCA CTCCCTACAG GAGATCACCA GCATGACCTT TAAAACGATG 
GCCATCCACA CCCTCTGGAA CATCCGCATC GTGGTGCTGG CCAAGCCGGA GCATGAGAAC 
CGCATCAGCC ACGTCTGCAC GAACCACGTC AAGACGGGCA TCGCCAACAC GCTGGGGAAC 
AAGGGAGCTG TGGGGGTATC CTTCATGTTC AATGGCACCT CCTTGGGATT TGTTAACAGC 
CACTTGACCT CAGGAAGCGA AAAGAAACTC AGGCGAAACC AAAACTATAC GAACATTCTC 
CGGTTTCTGG CCCTGGGAGA CAAGAAACTG AGTCCTTTTA ACATCACTCA CCGTTTCACC 
CATCTCTTCT GGCTTGGGGA TCTCAACTAC CGCTTGGAGC TGCCCATCTC GGAGGCAGAC 
ACCATCATCC AGAAAATCAA GCAGCAGCAG TATGCGGACC TCCTGGCCCA CGACCAGCTG 
CTCATGGAGA GGAAAGAGCA GAAGGTCTTC CTTCATTTCG ACGAGGAAGA AATCACATTC 
GCTCCCACCT ACCGTTTTGA AAGACTGACT CGGGACAAAT ACGCCTACAC TAAGCAGAAA 
GCAACAGGGA TGAAGTACAA CTTGCCCTCC TGGTGTGATC GGGTCCTCTG GAAGTCATAC 
CCGCTGGTCC ACGTGGTGTG TCAGTCCTAC GGCAGTACCA GCGACATTAT GACGAGTGAC 
CACAGCCCTG TCTTTGCCAC GTTTGAGGCA GGAGTCACCT CCCAGTTTGT CTCCAAGAAC 
GGCCCTGGGA CTGTGGACAG CCAAGGGCAA ATTGAGTTTC TCAGCTGCTA CGCCACGTTG 
AAGACCAAGT CCCAGACCAA ATTCTACCTT GAGTTCCACT CCAGCTGCTT GGAGAGCTTT 
GTCAAGAGTC AGGAAGGAGA AAACGAAGAA GGAAGCGAAG GAGAGTTGTT GGTGAAGTTT 
GGCGAGACCC TTCCAAAGCT GAAGCCCATT ATCTCTGACC CGGAATACCT GCTGGACCAG 
CACATCCTGA TCAGCATTAA GTCCTCTGAC AGTGACGAAT CCTACGGCGA GGGCTGCATT 
GCCCTACGGT TAGAGGCCAA GGAAACCCAG TTCCCCATCT ACACGCCTCT CACCCACCAC 
GGAGAGATGA TGGGACATTT CCAGGGGGAG ATTAAGCTGC AGACATCCGA AGGCAAGACG 
AGAGAGAAGC TCTACGACTT CGTGAAGACG GAGCGGGATG AGTCCAGCGG GCCCAAGTCT 
CTGAAGAGCT TCACCAGCCA TGACCCCATG AAGCTGTGGG AACCCACCAA CAGGGCCCCT 
CTGTGCAGTG GCCCCAGCAT CACCGAGATC ATTAACCCCA ACTACATGGG TGTGGGGCCC 
TTTGGGCCAC CGATGCCCCT GCACGGGAAG CAGGTCATCT CCCCCGACCA GCAGCCCACA 
GCCTGGAGCT ACGACCCACT TCCCAAGGAT GCCTCCCTTG GGCCCGGATG GGGGGAGAGT 
CCTCCAACAC CACCCTCCCA ACAGCCCGTA TCACCCAAGA AGTTTTCACC CTCTCCAGCC 
AGTCGGGGCC CCAGCCCCAG GACACAGGAG TCGAGTCTGA AAGCTCTGCT GAAACCTGCT 
CAGCATCATA GCAACACGCA AGCCTCCACT GACAGATGGG TGGTGGCTGT GCATGAGGTG 
CTTTGGCTGA GAACTGAACT CAAGTCTCTC ACACGGAAGG CAAGGATTCT ACCACCGAAC 
CACCGCTGCC TCCATTGGTG GAACACCGTA GGCACTGAGC CCAGCGACTT GGCGAAGAAC 
ATAGCAGAGC CCCTGCTTCA GGAGGACCTG CCGCTGACCA AGCAGAAGCC GGAGATGTTT 
GAGAACCCGC TGTACGGGTC TGTCAGCTCC TTCCCTAAGC TGGTTCCCAG GAAAGAGCAG 
GAATCCCCCA AGATGCTGAG GAAGGAGCCC CCACCCTGCC CAGACCCTGG AGTCTCCTCA 
CCCACCATCT TGCTCACCAA AGCGCAGGAG GCCGAGAGCT CCAAGGGGAC TAGCAAGCAG 
GTCCCCACGC CCCGGCTGCG CTCCTTCACC TGCTCGTCCT CCACCGCAGA GGCCGGAGCG 
GCGGCCGGCG GGGAGAAGAA CCACGGGAAG CCCAAGCCCG CGGCCAGCTC CCAGGCGCCG 
GCGCCCGTGC CGGCGAAGAG GCCCGTGAAG CCGTCCAGGT CAGAAATGAG CCAGCCGATC 
CCGCCCGCGC CCACGCAGCT CCGACCGCCC CTGCCCGCCA AAAGCCTGGC CGTGCTGCAC 
CTGCAGCACT CCAAGGGCCG CGACTACCGC GACAACACCG AGCTCTTGCA CCACGGCAAG 
CACCGGCCCG AGGAGGGGCC GCTCGGCAGG ACCGCCATGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023580268.1
CDS	645..4709
Translation	MDSFRTDETAAKPRCRPHVHFQMALLACSLSSLNAGGGGQEASSASVQDWLTSGLISNSEKGQLQDLDTSCPGSSSYVSNSCLFLVTRKMDFSAHVLMKPLISFRYRNCVYTYRILPNEDKFSVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQYPVPLEEEDASDEPEEETGRRGRQEAGQLAQQGGALGTTSSKEHGGEVLDVSLPSNACIEECDVSPPELPPRNIPLSAGPCEAKEVPLSSESPRVPEVSKPTLSETLLQRLQSIDTSGLPDEYLKAIQDYLSTQLVLDSEFVKTGSSSLPHLKKLTTLLCKELYGEVVRTLPTLEFLQRLFDQQLSPGLRPRPQVSGEANPINMVAKFSQLTSLLSSIEDKVKAMLHEGHESPHRCSIIPPVNFEVKSESLGIPQKLHLKVDVETGKLIVKKSKDGSEDKYYSHKKILQLVKSQKFLNKLVIVVETEKEKTVRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILKHSLQEITSMTFKTMAIHTLWNIRIVVLAKPEHENRISHVCTNHVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYTNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRLELPISEADTIIQKIKQQQYADLLAHDQLLMERKEQKVFLHFDEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLSCYATLKTKSQTKFYLEFHSSCLESFVKSQEGENEEGSEGELLVKFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLEAKETQFPIYTPLTHHGEMMGHFQGEIKLQTSEGKTREKLYDFVKTERDESSGPKSLKSFTSHDPMKLWEPTNRAPLCSGPSITEIINPNYMGVGPFGPPMPLHGKQVISPDQQPTAWSYDPLPKDASLGPGWGESPPTPPSQQPVSPKKFSPSPASRGPSPRTQESSLKALLKPAQHHSNTQASTDRWVVAVHEVLWLRTELKSLTRKARILPPNHRCLHWWNTVGTEPSDLAKNIAEPLLQEDLPLTKQKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPDPGVSSPTILLTKAQEAESSKGTSKQVPTPRLRSFTCSSSTAEAGAAAGGEKNHGKPKPAASSQAPAPVPAKRPVKPSRSEMSQPIPPAPTQLRPPLPAKSLAVLHLQHSKGRDYRDNTELLHHGKHRPEEGPLGRTAMQ

Target ORF information:

RefSeq Version	XM_023724500.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023724500.1

ORF Insert Sequence:

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGGTAG GCGGGGAAGG 
CAGGAGGCCG GGCAGCTGGC TCAGCAGGGA GGTGCCTTAG GGACCACGTC CTCCAAGGAA 
CATGGTGGAG AAGTGCTGGA TGTTTCTCTC CCCTCGAATG CTTGCATAGA AGAATGCGAC 
GTATCTCCAC CTGAGCTGCC CCCCAGGAAC ATCCCTTTGT CTGCTGGGCC CTGTGAAGCC 
AAGGAAGTTC CTCTTTCAAG CGAGAGTCCC CGAGTGCCCG AGGTCAGCAA GCCGACCCTC 
TCTGAGACGT TACTTCAGAG GCTGCAGAGC ATCGACACTA GTGGGCTTCC GGACGAGTAT 
CTTAAAGCCA TCCAGGATTA TTTAAGCACG CAGCTAGTCC TCGACTCTGA GTTTGTGAAG 
ACGGGCTCCA GCAGTCTCCC TCACCTGAAG AAACTGACCA CGCTCCTGTG CAAGGAGCTT 
TATGGCGAGG TCGTTCGGAC TCTTCCCACT CTGGAGTTTC TGCAGAGGCT GTTCGACCAG 
CAGCTGTCCC CTGGCCTCCG CCCACGTCCT CAGGTGTCTG GTGAGGCCAA TCCCATCAAC 
ATGGTGGCCA AGTTCAGCCA ACTGACAAGT CTGCTGTCCT CTATTGAAGA TAAGGTCAAG 
GCCATGCTGC ATGAGGGTCA TGAGTCTCCT CACCGGTGCT CCATCATCCC TCCGGTCAAC 
TTCGAGGTAA AGTCAGAGTC CTTGGGGATT CCTCAGAAAT TGCATCTCAA GGTCGACGTT 
GAGACTGGGA AACTGATCGT TAAGAAGTCC AAGGATGGTT CTGAGGACAA GTACTACAGC 
CACAAGAAAA TCCTGCAGCT CGTCAAGTCT CAGAAGTTTC TTAACAAGCT GGTGATTGTG 
GTGGAAACGG AGAAGGAGAA GACCGTGCGG AAGGAATATG TCTTTGCTGA CTCTAAAAAG 
AGAGAAGGCT TCTGCCAGCT TCTCCAGCAG ATGAAGAACA AGCACTCGGA GCAGCCTGAG 
CCCGACATGA TCACCATCTT CATCGGCACC TGGAACATGG GTAACGCCCC CCCTCCCAAG 
AAGATCACGT CCTGGTTTCT CTCCAAGGGG CAGGGAAAGA CGCGGGATGA CTCAGCAGAC 
TACATCCCCC ATGACATCTA TGTGATCGGC ACCCAGGAGG ACCCCCTGGG AGAAAAGGAG 
TGGCTGGAGA TCCTCAAGCA CTCCCTACAG GAGATCACCA GCATGACCTT TAAAACGATG 
GCCATCCACA CCCTCTGGAA CATCCGCATC GTGGTGCTGG CCAAGCCGGA GCATGAGAAC 
CGCATCAGCC ACGTCTGCAC GAACCACGTC AAGACGGGCA TCGCCAACAC GCTGGGGAAC 
AAGGGAGCTG TGGGGGTATC CTTCATGTTC AATGGCACCT CCTTGGGATT TGTTAACAGC 
CACTTGACCT CAGGAAGCGA AAAGAAACTC AGGCGAAACC AAAACTATAC GAACATTCTC 
CGGTTTCTGG CCCTGGGAGA CAAGAAACTG AGTCCTTTTA ACATCACTCA CCGTTTCACC 
CATCTCTTCT GGCTTGGGGA TCTCAACTAC CGCTTGGAGC TGCCCATCTC GGAGGCAGAC 
ACCATCATCC AGAAAATCAA GCAGCAGCAG TATGCGGACC TCCTGGCCCA CGACCAGCTG 
CTCATGGAGA GGAAAGAGCA GAAGGTCTTC CTTCATTTCG ACGAGGAAGA AATCACATTC 
GCTCCCACCT ACCGTTTTGA AAGACTGACT CGGGACAAAT ACGCCTACAC TAAGCAGAAA 
GCAACAGGGA TGAAGTACAA CTTGCCCTCC TGGTGTGATC GGGTCCTCTG GAAGTCATAC 
CCGCTGGTCC ACGTGGTGTG TCAGTCCTAC GGCAGTACCA GCGACATTAT GACGAGTGAC 
CACAGCCCTG TCTTTGCCAC GTTTGAGGCA GGAGTCACCT CCCAGTTTGT CTCCAAGAAC 
GGCCCTGGGA CTGTGGACAG CCAAGGGCAA ATTGAGTTTC TCAGCTGCTA CGCCACGTTG 
AAGACCAAGT CCCAGACCAA ATTCTACCTT GAGTTCCACT CCAGCTGCTT GGAGAGCTTT 
GTCAAGAGTC AGGAAGGAGA AAACGAAGAA GGAAGCGAAG GAGAGTTGTT GGTGAAGTTT 
GGCGAGACCC TTCCAAAGCT GAAGCCCATT ATCTCTGACC CGGAATACCT GCTGGACCAG 
CACATCCTGA TCAGCATTAA GTCCTCTGAC AGTGACGAAT CCTACGGCGA GGGCTGCATT 
GCCCTACGGT TAGAGGCCAA GGAAACCCAG TTCCCCATCT ACACGCCTCT CACCCACCAC 
GGAGAGATGA TGGGACATTT CCAGGGGGAG ATTAAGCTGC AGACATCCGA AGGCAAGACG 
AGAGAGAAGC TCTACGACTT CGTGAAGACG GAGCGGGATG AGTCCAGCGG GCCCAAGTCT 
CTGAAGAGCT TCACCAGCCA TGACCCCATG AAGCTGTGGG AACCCACCAA CAGGGCCCCT 
CTGTGCAGTG GCCCCAGCAT CACCGAGATC ATTAACCCCA ACTACATGGG TGTGGGGCCC 
TTTGGGCCAC CGATGCCCCT GCACGGGAAG CAGGTCATCT CCCCCGACCA GCAGCCCACA 
GCCTGGAGCT ACGACCCACT TCCCAAGGAT GCCTCCCTTG GGCCCGGATG GGGGGAGAGT 
CCTCCAACAC CACCCTCCCA ACAGCCCGTA TCACCCAAGA AGTTTTCACC CTCTCCAGCC 
AGTCGGGGCC CCAGCCCCAG GACACAGGAG TCGAGTCTGA AAGCTCTGCT GAAACCTGCT 
CAGCATCATA GCAACACGCA AGCCTCCACT GACAGATGGG TGGTGGCTGT GCATGAGGTG 
CTTTGGCTGA GAACTGAACT CAAGTCTCTC ACACGGAAGG CAAGGATTCT ACCACCGAAC 
CACCGCTGCC TCCATTGGTG GAACACCGTA GGCACTGAGC CCAGCGACTT GGCGAAGAAC 
ATAGCAGAGC CCCTGCTTCA GGAGGACCTG CCGCTGACCA AGCAGAAGCC GGAGATGTTT 
GAGAACCCGC TGTACGGGTC TGTCAGCTCC TTCCCTAAGC TGGTTCCCAG GAAAGAGCAG 
GAATCCCCCA AGATGCTGAG GAAGGAGCCC CCACCCTGCC CAGACCCTGG AGTCTCCTCA 
CCCACCATCT TGCTCACCAA AGCGCAGGAG GCCGAGAGCT CCAAGGGGAC TAGCAAGCAG 
GTCCCCACGC CCCGGCTGCG CTCCTTCACC TGCTCGTCCT CCACCGCAGA GGCCGGAGCG 
GCGGCCGGCG GGGAGAAGAA CCACGGGAAG CCCAAGCCCG CGGCCAGCTC CCAGGCGCCG 
GCGCCCGTGC CGGCGAAGAG GCCCGTGAAG CCGTCCAGGT CAGAAATGAG CCAGCCGATC 
CCGCCCGCGC CCACGCAGCT CCGACCGCCC CTGCCCGCCA AAAGCCTGGC CGTGCTGCAC 
CTGCAGCACT CCAAGGGCCG CGACTACCGC GACAACACCG AGCTCTTGCA CCACGGCAAG 
CACCGGCCCG AGGAGGGGCC GCTCGGCAGG ACCGCCATGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101350772 ( XM_023724502.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023580270.1 Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTr227349
Clone ID Related Accession (Same CDS sequence)	XM_023724502.1
Accession Version	XM_023724502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444185.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGGTAG GCGGGGAAGG 
CAGGAGGCCG GGCAGCTGGC TCAGCAGGGA GGTGCCTTAG GGACCACGTC CTCCAAGGAA 
CATGGTGGAG AAGTGCTGGA TGTTTCTCTC CCCTCGAATG CTTGCATAGA AGAATGCGAC 
GTATCTCCAC CTGAGCTGCC CCCCAGGAAC ATCCCTTTGT CTGCTGGGCC CTGTGAAGCC 
AAGGAAGTTC CTCTTTCAAG CGAGAGTCCC CGAGTGCCCG AGGTCAGCAA GCCGACCCTC 
TCTGAGACGT TACTTCAGAG GCTGCAGAGC ATCGACACTA GTGGGCTTCC GGACGAGTAT 
CTTAAAGCCA TCCAGGATTA TTTAAGCACG CAGCTAGTCC TCGACTCTGA GTTTGTGAAG 
ACGGGCTCCA GCAGTCTCCC TCACCTGAAG AAACTGACCA CGCTCCTGTG CAAGGAGCTT 
TATGGCGAGG TCGTTCGGAC TCTTCCCACT CTGGAGTTTC TGCAGAGGCT GTTCGACCAG 
CAGCTGTCCC CTGGCCTCCG CCCACGTCCT CAGGTGTCTG GTGAGGCCAA TCCCATCAAC 
ATGGTGGCCA AGTTCAGCCA ACTGACAAGT CTGCTGTCCT CTATTGAAGA TAAGGTCAAG 
GCCATGCTGC ATGAGGGTCA TGAGTCTCCT CACCGGTGCT CCATCATCCC TCCGGTCAAC 
TTCGAGGTAA AGTCAGAGTC CTTGGGGATT CCTCAGAAAT TGCATCTCAA GGTCGACGTT 
GAGACTGGGA AACTGATCGT TAAGAAGTCC AAGGATGGTT CTGAGGACAA GTACTACAGC 
CACAAGAAAA TCCTGCAGCT CGTCAAGTCT CAGAAGTTTC TTAACAAGCT GGTGATTGTG 
GTGGAAACGG AGAAGGAGAA GACCGTGCGG AAGGAATATG TCTTTGCTGA CTCTAAAAAG 
AGAGAAGGCT TCTGCCAGCT TCTCCAGCAG ATGAAGAACA AGCACTCGGA GCAGCCTGAG 
CCCGACATGA TCACCATCTT CATCGGCACC TGGAACATGG GTAACGCCCC CCCTCCCAAG 
AAGATCACGT CCTGGTTTCT CTCCAAGGGG CAGGGAAAGA CGCGGGATGA CTCAGCAGAC 
TACATCCCCC ATGACATCTA TGTGATCGGC ACCCAGGAGG ACCCCCTGGG AGAAAAGGAG 
TGGCTGGAGA TCCTCAAGCA CTCCCTACAG GAGATCACCA GCATGACCTT TAAAACGATG 
GCCATCCACA CCCTCTGGAA CATCCGCATC GTGGTGCTGG CCAAGCCGGA GCATGAGAAC 
CGCATCAGCC ACGTCTGCAC GAACCACGTC AAGACGGGCA TCGCCAACAC GCTGGGGAAC 
AAGGGAGCTG TGGGGGTATC CTTCATGTTC AATGGCACCT CCTTGGGATT TGTTAACAGC 
CACTTGACCT CAGGAAGCGA AAAGAAACTC AGGCGAAACC AAAACTATAC GAACATTCTC 
CGGTTTCTGG CCCTGGGAGA CAAGAAACTG AGTCCTTTTA ACATCACTCA CCGTTTCACC 
CATCTCTTCT GGCTTGGGGA TCTCAACTAC CGCTTGGAGC TGCCCATCTC GGAGGCAGAC 
ACCATCATCC AGAAAATCAA GCAGCAGCAG TATGCGGACC TCCTGGCCCA CGACCAGCTG 
CTCATGGAGA GGAAAGAGCA GAAGGTCTTC CTTCATTTCG ACGAGGAAGA AATCACATTC 
GCTCCCACCT ACCGTTTTGA AAGACTGACT CGGGACAAAT ACGCCTACAC TAAGCAGAAA 
GCAACAGGGA TGAAGTACAA CTTGCCCTCC TGGTGTGATC GGGTCCTCTG GAAGTCATAC 
CCGCTGGTCC ACGTGGTGTG TCAGTCCTAC GGCAGTACCA GCGACATTAT GACGAGTGAC 
CACAGCCCTG TCTTTGCCAC GTTTGAGGCA GGAGTCACCT CCCAGTTTGT CTCCAAGAAC 
GGCCCTGGGA CTGTGGACAG CCAAGGGCAA ATTGAGTTTC TCAGCTGCTA CGCCACGTTG 
AAGACCAAGT CCCAGACCAA ATTCTACCTT GAGTTCCACT CCAGCTGCTT GGAGAGCTTT 
GTCAAGAGTC AGGAAGGAGA AAACGAAGAA GGAAGCGAAG GAGAGTTGTT GGTGAAGTTT 
GGCGAGACCC TTCCAAAGCT GAAGCCCATT ATCTCTGACC CGGAATACCT GCTGGACCAG 
CACATCCTGA TCAGCATTAA GTCCTCTGAC AGTGACGAAT CCTACGGCGA GGGCTGCATT 
GCCCTACGGT TAGAGGCCAA GGAAACCCAG TTCCCCATCT ACACGCCTCT CACCCACCAC 
GGAGAGATGA TGGGACATTT CCAGGGGGAG ATTAAGCTGC AGACATCCGA AGGCAAGACG 
AGAGAGAAGC TCTACGACTT CGTGAAGACG GAGCGGGATG AGTCCAGCGG GCCCAAGTCT 
CTGAAGAGCT TCACCAGCCA TGACCCCATG AAGCTGTGGG AACCCACCAA CAGGGCCCCT 
CTGTGCAGTG GCCCCAGCAT CACCGAGATC ATTAACCCCA ACTACATGGG TGTGGGGCCC 
TTTGGGCCAC CGATGCCCCT GCACGGGAAG CAGGTCATCT CCCCCGACCA GCAGCCCACA 
GCCTGGAGCT ACGACCCACT TCCCAAGGAT GCCTCCCTTG GGCCCGGATG GGGGGAGAGT 
CCTCCAACAC CACCCTCCCA ACAGCCCGTA TCACCCAAGA AGTTTTCACC CTCTCCAGCC 
AGTCGGGGCC CCAGCCCCAG GACACAGGAG TCGAGGCCCA GCGACTTGGC GAAGAACATA 
GCAGAGCCCC TGCTTCAGGA GGACCTGCCG CTGACCAAGC AGAAGCCGGA GATGTTTGAG 
AACCCGCTGT ACGGGTCTGT CAGCTCCTTC CCTAAGCTGG TTCCCAGGAA AGAGCAGGAA 
TCCCCCAAGA TGCTGAGGAA GGAGCCCCCA CCCTGCCCAG ACCCTGGAGT CTCCTCACCC 
ACCATCTTGC TCACCAAAGC GCAGGAGGCC GAGAGCTCCA AGGGGACTAG CAAGCAGGTC 
CCCACGCCCC GGCTGCGCTC CTTCACCTGC TCGTCCTCCA CCGCAGAGGC CGGAGCGGCG 
GCCGGCGGGG AGAAGAACCA CGGGAAGCCC AAGCCCGCGG CCAGCTCCCA GGCGCCGGCG 
CCCGTGCCGG CGAAGAGGCC CGTGAAGCCG TCCAGGTCAG AAATGAGCCA GCCGATCCCG 
CCCGCGCCCA CGCAGCTCCG ACCGCCCCTG CCCGCCAAAA GCCTGGCCGT GCTGCACCTG 
CAGCACTCCA AGGGCCGCGA CTACCGCGAC AACACCGAGC TCTTGCACCA CGGCAAGCAC 
CGGCCCGAGG AGGGGCCGCT CGGCAGGACC GCCATGCAGG TGCGCGCAGT GAAGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023580270.1
CDS	645..4544
Translation	MDSFRTDETAAKPRCRPHVHFQMALLACSLSSLNAGGGGQEASSASVQDWLTSGLISNSEKGQLQDLDTSCPGSSSYVSNSCLFLVTRKMDFSAHVLMKPLISFRYRNCVYTYRILPNEDKFSVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQYPVPLEEEDASDEPEEETGRRGRQEAGQLAQQGGALGTTSSKEHGGEVLDVSLPSNACIEECDVSPPELPPRNIPLSAGPCEAKEVPLSSESPRVPEVSKPTLSETLLQRLQSIDTSGLPDEYLKAIQDYLSTQLVLDSEFVKTGSSSLPHLKKLTTLLCKELYGEVVRTLPTLEFLQRLFDQQLSPGLRPRPQVSGEANPINMVAKFSQLTSLLSSIEDKVKAMLHEGHESPHRCSIIPPVNFEVKSESLGIPQKLHLKVDVETGKLIVKKSKDGSEDKYYSHKKILQLVKSQKFLNKLVIVVETEKEKTVRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILKHSLQEITSMTFKTMAIHTLWNIRIVVLAKPEHENRISHVCTNHVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYTNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRLELPISEADTIIQKIKQQQYADLLAHDQLLMERKEQKVFLHFDEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLSCYATLKTKSQTKFYLEFHSSCLESFVKSQEGENEEGSEGELLVKFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLEAKETQFPIYTPLTHHGEMMGHFQGEIKLQTSEGKTREKLYDFVKTERDESSGPKSLKSFTSHDPMKLWEPTNRAPLCSGPSITEIINPNYMGVGPFGPPMPLHGKQVISPDQQPTAWSYDPLPKDASLGPGWGESPPTPPSQQPVSPKKFSPSPASRGPSPRTQESRPSDLAKNIAEPLLQEDLPLTKQKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPDPGVSSPTILLTKAQEAESSKGTSKQVPTPRLRSFTCSSSTAEAGAAAGGEKNHGKPKPAASSQAPAPVPAKRPVKPSRSEMSQPIPPAPTQLRPPLPAKSLAVLHLQHSKGRDYRDNTELLHHGKHRPEEGPLGRTAMQVRAVKP

Target ORF information:

RefSeq Version	XM_023724502.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023724502.1

ORF Insert Sequence:

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGGTAG GCGGGGAAGG 
CAGGAGGCCG GGCAGCTGGC TCAGCAGGGA GGTGCCTTAG GGACCACGTC CTCCAAGGAA 
CATGGTGGAG AAGTGCTGGA TGTTTCTCTC CCCTCGAATG CTTGCATAGA AGAATGCGAC 
GTATCTCCAC CTGAGCTGCC CCCCAGGAAC ATCCCTTTGT CTGCTGGGCC CTGTGAAGCC 
AAGGAAGTTC CTCTTTCAAG CGAGAGTCCC CGAGTGCCCG AGGTCAGCAA GCCGACCCTC 
TCTGAGACGT TACTTCAGAG GCTGCAGAGC ATCGACACTA GTGGGCTTCC GGACGAGTAT 
CTTAAAGCCA TCCAGGATTA TTTAAGCACG CAGCTAGTCC TCGACTCTGA GTTTGTGAAG 
ACGGGCTCCA GCAGTCTCCC TCACCTGAAG AAACTGACCA CGCTCCTGTG CAAGGAGCTT 
TATGGCGAGG TCGTTCGGAC TCTTCCCACT CTGGAGTTTC TGCAGAGGCT GTTCGACCAG 
CAGCTGTCCC CTGGCCTCCG CCCACGTCCT CAGGTGTCTG GTGAGGCCAA TCCCATCAAC 
ATGGTGGCCA AGTTCAGCCA ACTGACAAGT CTGCTGTCCT CTATTGAAGA TAAGGTCAAG 
GCCATGCTGC ATGAGGGTCA TGAGTCTCCT CACCGGTGCT CCATCATCCC TCCGGTCAAC 
TTCGAGGTAA AGTCAGAGTC CTTGGGGATT CCTCAGAAAT TGCATCTCAA GGTCGACGTT 
GAGACTGGGA AACTGATCGT TAAGAAGTCC AAGGATGGTT CTGAGGACAA GTACTACAGC 
CACAAGAAAA TCCTGCAGCT CGTCAAGTCT CAGAAGTTTC TTAACAAGCT GGTGATTGTG 
GTGGAAACGG AGAAGGAGAA GACCGTGCGG AAGGAATATG TCTTTGCTGA CTCTAAAAAG 
AGAGAAGGCT TCTGCCAGCT TCTCCAGCAG ATGAAGAACA AGCACTCGGA GCAGCCTGAG 
CCCGACATGA TCACCATCTT CATCGGCACC TGGAACATGG GTAACGCCCC CCCTCCCAAG 
AAGATCACGT CCTGGTTTCT CTCCAAGGGG CAGGGAAAGA CGCGGGATGA CTCAGCAGAC 
TACATCCCCC ATGACATCTA TGTGATCGGC ACCCAGGAGG ACCCCCTGGG AGAAAAGGAG 
TGGCTGGAGA TCCTCAAGCA CTCCCTACAG GAGATCACCA GCATGACCTT TAAAACGATG 
GCCATCCACA CCCTCTGGAA CATCCGCATC GTGGTGCTGG CCAAGCCGGA GCATGAGAAC 
CGCATCAGCC ACGTCTGCAC GAACCACGTC AAGACGGGCA TCGCCAACAC GCTGGGGAAC 
AAGGGAGCTG TGGGGGTATC CTTCATGTTC AATGGCACCT CCTTGGGATT TGTTAACAGC 
CACTTGACCT CAGGAAGCGA AAAGAAACTC AGGCGAAACC AAAACTATAC GAACATTCTC 
CGGTTTCTGG CCCTGGGAGA CAAGAAACTG AGTCCTTTTA ACATCACTCA CCGTTTCACC 
CATCTCTTCT GGCTTGGGGA TCTCAACTAC CGCTTGGAGC TGCCCATCTC GGAGGCAGAC 
ACCATCATCC AGAAAATCAA GCAGCAGCAG TATGCGGACC TCCTGGCCCA CGACCAGCTG 
CTCATGGAGA GGAAAGAGCA GAAGGTCTTC CTTCATTTCG ACGAGGAAGA AATCACATTC 
GCTCCCACCT ACCGTTTTGA AAGACTGACT CGGGACAAAT ACGCCTACAC TAAGCAGAAA 
GCAACAGGGA TGAAGTACAA CTTGCCCTCC TGGTGTGATC GGGTCCTCTG GAAGTCATAC 
CCGCTGGTCC ACGTGGTGTG TCAGTCCTAC GGCAGTACCA GCGACATTAT GACGAGTGAC 
CACAGCCCTG TCTTTGCCAC GTTTGAGGCA GGAGTCACCT CCCAGTTTGT CTCCAAGAAC 
GGCCCTGGGA CTGTGGACAG CCAAGGGCAA ATTGAGTTTC TCAGCTGCTA CGCCACGTTG 
AAGACCAAGT CCCAGACCAA ATTCTACCTT GAGTTCCACT CCAGCTGCTT GGAGAGCTTT 
GTCAAGAGTC AGGAAGGAGA AAACGAAGAA GGAAGCGAAG GAGAGTTGTT GGTGAAGTTT 
GGCGAGACCC TTCCAAAGCT GAAGCCCATT ATCTCTGACC CGGAATACCT GCTGGACCAG 
CACATCCTGA TCAGCATTAA GTCCTCTGAC AGTGACGAAT CCTACGGCGA GGGCTGCATT 
GCCCTACGGT TAGAGGCCAA GGAAACCCAG TTCCCCATCT ACACGCCTCT CACCCACCAC 
GGAGAGATGA TGGGACATTT CCAGGGGGAG ATTAAGCTGC AGACATCCGA AGGCAAGACG 
AGAGAGAAGC TCTACGACTT CGTGAAGACG GAGCGGGATG AGTCCAGCGG GCCCAAGTCT 
CTGAAGAGCT TCACCAGCCA TGACCCCATG AAGCTGTGGG AACCCACCAA CAGGGCCCCT 
CTGTGCAGTG GCCCCAGCAT CACCGAGATC ATTAACCCCA ACTACATGGG TGTGGGGCCC 
TTTGGGCCAC CGATGCCCCT GCACGGGAAG CAGGTCATCT CCCCCGACCA GCAGCCCACA 
GCCTGGAGCT ACGACCCACT TCCCAAGGAT GCCTCCCTTG GGCCCGGATG GGGGGAGAGT 
CCTCCAACAC CACCCTCCCA ACAGCCCGTA TCACCCAAGA AGTTTTCACC CTCTCCAGCC 
AGTCGGGGCC CCAGCCCCAG GACACAGGAG TCGAGGCCCA GCGACTTGGC GAAGAACATA 
GCAGAGCCCC TGCTTCAGGA GGACCTGCCG CTGACCAAGC AGAAGCCGGA GATGTTTGAG 
AACCCGCTGT ACGGGTCTGT CAGCTCCTTC CCTAAGCTGG TTCCCAGGAA AGAGCAGGAA 
TCCCCCAAGA TGCTGAGGAA GGAGCCCCCA CCCTGCCCAG ACCCTGGAGT CTCCTCACCC 
ACCATCTTGC TCACCAAAGC GCAGGAGGCC GAGAGCTCCA AGGGGACTAG CAAGCAGGTC 
CCCACGCCCC GGCTGCGCTC CTTCACCTGC TCGTCCTCCA CCGCAGAGGC CGGAGCGGCG 
GCCGGCGGGG AGAAGAACCA CGGGAAGCCC AAGCCCGCGG CCAGCTCCCA GGCGCCGGCG 
CCCGTGCCGG CGAAGAGGCC CGTGAAGCCG TCCAGGTCAG AAATGAGCCA GCCGATCCCG 
CCCGCGCCCA CGCAGCTCCG ACCGCCCCTG CCCGCCAAAA GCCTGGCCGT GCTGCACCTG 
CAGCACTCCA AGGGCCGCGA CTACCGCGAC AACACCGAGC TCTTGCACCA CGGCAAGCAC 
CGGCCCGAGG AGGGGCCGCT CGGCAGGACC GCCATGCAGG TGCGCGCAGT GAAGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101350772 ( XM_023724503.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023580271.1 Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTr227350
Clone ID Related Accession (Same CDS sequence)	XM_023724503.1
Accession Version	XM_023724503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444185.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGGTGCGCG CAGTGAAGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023580271.1
CDS	645..4418
Translation	MDSFRTDETAAKPRCRPHVHFQMALLACSLSSLNAGGGGQEASSASVQDWLTSGLISNSEKGQLQDLDTSCPGSSSYVSNSCLFLVTRKMDFSAHVLMKPLISFRYRNCVYTYRILPNEDKFSVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQYPVPLEEEDASDEPEEETECDVSPPELPPRNIPLSAGPCEAKEVPLSSESPRVPEVSKPTLSETLLQRLQSIDTSGLPDEYLKAIQDYLSTQLVLDSEFVKTGSSSLPHLKKLTTLLCKELYGEVVRTLPTLEFLQRLFDQQLSPGLRPRPQVSGEANPINMVAKFSQLTSLLSSIEDKVKAMLHEGHESPHRCSIIPPVNFEVKSESLGIPQKLHLKVDVETGKLIVKKSKDGSEDKYYSHKKILQLVKSQKFLNKLVIVVETEKEKTVRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILKHSLQEITSMTFKTMAIHTLWNIRIVVLAKPEHENRISHVCTNHVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYTNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRLELPISEADTIIQKIKQQQYADLLAHDQLLMERKEQKVFLHFDEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLSCYATLKTKSQTKFYLEFHSSCLESFVKSQEGENEEGSEGELLVKFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLEAKETQFPIYTPLTHHGEMMGHFQGEIKLQTSEGKTREKLYDFVKTERDESSGPKSLKSFTSHDPMKLWEPTNRAPLCSGPSITEIINPNYMGVGPFGPPMPLHGKQVISPDQQPTAWSYDPLPKDASLGPGWGESPPTPPSQQPVSPKKFSPSPASRGPSPRTQESRPSDLAKNIAEPLLQEDLPLTKQKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPDPGVSSPTILLTKAQEAESSKGTSKQVPTPRLRSFTCSSSTAEAGAAAGGEKNHGKPKPAASSQAPAPVPAKRPVKPSRSEMSQPIPPAPTQLRPPLPAKSLAVLHLQHSKGRDYRDNTELLHHGKHRPEEGPLGRTAMQVRAVKP

Target ORF information:

RefSeq Version	XM_023724503.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023724503.1

ORF Insert Sequence:

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGGTGCGCG CAGTGAAGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101350772 ( XM_023724504.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023580272.1 Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X4, mRNA.

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CloneID	OTr227351
Clone ID Related Accession (Same CDS sequence)	XM_023724504.1
Accession Version	XM_023724504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444185.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGGTAG GCGGGGAAGG 
CAGGAGGCCG GGCAGCTGGC TCAGCAGGGA GGTGCCTTAG GGACCACGTC CTCCAAGGAA 
CATGGTGGAG AAGTGCTGGA TGTTTCTCTC CCCTCGAATG CTTGCATAGA AGAATGCGAC 
GTATCTCCAC CTGAGCTGCC CCCCAGGAAC ATCCCTTTGT CTGCTGGGCC CTGTGAAGCC 
AAGGAAGTTC CTCTTTCAAG CGAGAGTCCC CGAGTGCCCG AGGTCAGCAA GCCGACCCTC 
TCTGAGACGT TACTTCAGAG GCTGCAGAGC ATCGACACTA GTGGGCTTCC GGACGAGTAT 
CTTAAAGCCA TCCAGGATTA TTTAAGCACG CAGCTAGTCC TCGACTCTGA GTTTGTGAAG 
ACGGGCTCCA GCAGTCTCCC TCACCTGAAG AAACTGACCA CGCTCCTGTG CAAGGAGCTT 
TATGGCGAGG TCGTTCGGAC TCTTCCCACT CTGGAGTTTC TGCAGAGGCT GTTCGACCAG 
CAGCTGTCCC CTGGCCTCCG CCCACGTCCT CAGGTGTCTG GTGAGGCCAA TCCCATCAAC 
ATGGTGGCCA AGTTCAGCCA ACTGACAAGT CTGCTGTCCT CTATTGAAGA TAAGGTCAAG 
GCCATGCTGC ATGAGGGTCA TGAGTCTCCT CACCGGTGCT CCATCATCCC TCCGGTCAAC 
TTCGAGGTAA AGTCAGAGTC CTTGGGGATT CCTCAGAAAT TGCATCTCAA GGTCGACGTT 
GAGACTGGGA AACTGATCGT TAAGAAGTCC AAGGATGGTT CTGAGGACAA GTACTACAGC 
CACAAGAAAA TCCTGCAGCT CGTCAAGTCT CAGAAGTTTC TTAACAAGCT GGTGATTGTG 
GTGGAAACGG AGAAGGAGAA GACCGTGCGG AAGGAATATG TCTTTGCTGA CTCTAAAAAG 
AGAGAAGGCT TCTGCCAGCT TCTCCAGCAG ATGAAGAACA AGCACTCGGA GCAGCCTGAG 
CCCGACATGA TCACCATCTT CATCGGCACC TGGAACATGG GTAACGCCCC CCCTCCCAAG 
AAGATCACGT CCTGGTTTCT CTCCAAGGGG CAGGGAAAGA CGCGGGATGA CTCAGCAGAC 
TACATCCCCC ATGACATCTA TGTGATCGGC ACCCAGGAGG ACCCCCTGGG AGAAAAGGAG 
TGGCTGGAGA TCCTCAAGCA CTCCCTACAG GAGATCACCA GCATGACCTT TAAAACGATG 
GCCATCCACA CCCTCTGGAA CATCCGCATC GTGGTGCTGG CCAAGCCGGA GCATGAGAAC 
CGCATCAGCC ACGTCTGCAC GAACCACGTC AAGACGGGCA TCGCCAACAC GCTGGGGAAC 
AAGGGAGCTG TGGGGGTATC CTTCATGTTC AATGGCACCT CCTTGGGATT TGTTAACAGC 
CACTTGACCT CAGGAAGCGA AAAGAAACTC AGGCGAAACC AAAACTATAC GAACATTCTC 
CGGTTTCTGG CCCTGGGAGA CAAGAAACTG AGTCCTTTTA ACATCACTCA CCGTTTCACC 
CATCTCTTCT GGCTTGGGGA TCTCAACTAC CGCTTGGAGC TGCCCATCTC GGAGGCAGAC 
ACCATCATCC AGAAAATCAA GCAGCAGCAG TATGCGGACC TCCTGGCCCA CGACCAGCTG 
CTCATGGAGA GGAAAGAGCA GAAGGTCTTC CTTCATTTCG ACGAGGAAGA AATCACATTC 
GCTCCCACCT ACCGTTTTGA AAGACTGACT CGGGACAAAT ACGCCTACAC TAAGCAGAAA 
GCAACAGGGA TGAAGTACAA CTTGCCCTCC TGGTGTGATC GGGTCCTCTG GAAGTCATAC 
CCGCTGGTCC ACGTGGTGTG TCAGTCCTAC GGCAGTACCA GCGACATTAT GACGAGTGAC 
CACAGCCCTG TCTTTGCCAC GTTTGAGGCA GGAGTCACCT CCCAGTTTGT CTCCAAGAAC 
GGCCCTGGGA CTGTGGACAG CCAAGGGCAA ATTGAGTTTC TCAGCTGCTA CGCCACGTTG 
AAGACCAAGT CCCAGACCAA ATTCTACCTT GAGTTCCACT CCAGCTGCTT GGAGAGCTTT 
GTCAAGAGTC AGGAAGGAGA AAACGAAGAA GGAAGCGAAG GAGAGTTGTT GGTGAAGTTT 
GGCGAGACCC TTCCAAAGCT GAAGCCCATT ATCTCTGACC CGGAATACCT GCTGGACCAG 
CACATCCTGA TCAGCATTAA GTCCTCTGAC AGTGACGAAT CCTACGGCGA GGGCTGCATT 
GCCCTACGGT TAGAGGCCAA GGAAACCCAG TTCCCCATCT ACACGCCTCT CACCCACCAC 
GGAGAGATGA TGGGACATTT CCAGGGGGAG ATTAAGCTGC AGACATCCGA AGGCAAGACG 
AGAGAGAAGC TCTACGACTT CGTGAAGACG GAGCGGGATG AGTCCAGCGG GCCCAAGTCT 
CTGAAGAGCT TCACCAGCCA TGACCCCATG AAGCTGTGGG AACCCACCAA CAGGCCCAGC 
GACTTGGCGA AGAACATAGC AGAGCCCCTG CTTCAGGAGG ACCTGCCGCT GACCAAGCAG 
AAGCCGGAGA TGTTTGAGAA CCCGCTGTAC GGGTCTGTCA GCTCCTTCCC TAAGCTGGTT 
CCCAGGAAAG AGCAGGAATC CCCCAAGATG CTGAGGAAGG AGCCCCCACC CTGCCCAGAC 
CCTGGAGTCT CCTCACCCAC CATCTTGCTC ACCAAAGCGC AGGAGGCCGA GAGCTCCAAG 
GGGACTAGCA AGCAGGTCCC CACGCCCCGG CTGCGCTCCT TCACCTGCTC GTCCTCCACC 
GCAGAGGCCG GAGCGGCGGC CGGCGGGGAG AAGAACCACG GGAAGCCCAA GCCCGCGGCC 
AGCTCCCAGG CGCCGGCGCC CGTGCCGGCG AAGAGGCCCG TGAAGCCGTC CAGGTCAGAA 
ATGAGCCAGC CGATCCCGCC CGCGCCCACG CAGCTCCGAC CGCCCCTGCC CGCCAAAAGC 
CTGGCCGTGC TGCACCTGCA GCACTCCAAG GGCCGCGACT ACCGCGACAA CACCGAGCTC 
TTGCACCACG GCAAGCACCG GCCCGAGGAG GGGCCGCTCG GCAGGACCGC CATGCAGGTG 
CGCGCAGTGA AGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023580272.1
CDS	645..4262
Translation	MDSFRTDETAAKPRCRPHVHFQMALLACSLSSLNAGGGGQEASSASVQDWLTSGLISNSEKGQLQDLDTSCPGSSSYVSNSCLFLVTRKMDFSAHVLMKPLISFRYRNCVYTYRILPNEDKFSVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQYPVPLEEEDASDEPEEETGRRGRQEAGQLAQQGGALGTTSSKEHGGEVLDVSLPSNACIEECDVSPPELPPRNIPLSAGPCEAKEVPLSSESPRVPEVSKPTLSETLLQRLQSIDTSGLPDEYLKAIQDYLSTQLVLDSEFVKTGSSSLPHLKKLTTLLCKELYGEVVRTLPTLEFLQRLFDQQLSPGLRPRPQVSGEANPINMVAKFSQLTSLLSSIEDKVKAMLHEGHESPHRCSIIPPVNFEVKSESLGIPQKLHLKVDVETGKLIVKKSKDGSEDKYYSHKKILQLVKSQKFLNKLVIVVETEKEKTVRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILKHSLQEITSMTFKTMAIHTLWNIRIVVLAKPEHENRISHVCTNHVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYTNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRLELPISEADTIIQKIKQQQYADLLAHDQLLMERKEQKVFLHFDEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLSCYATLKTKSQTKFYLEFHSSCLESFVKSQEGENEEGSEGELLVKFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLEAKETQFPIYTPLTHHGEMMGHFQGEIKLQTSEGKTREKLYDFVKTERDESSGPKSLKSFTSHDPMKLWEPTNRPSDLAKNIAEPLLQEDLPLTKQKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPDPGVSSPTILLTKAQEAESSKGTSKQVPTPRLRSFTCSSSTAEAGAAAGGEKNHGKPKPAASSQAPAPVPAKRPVKPSRSEMSQPIPPAPTQLRPPLPAKSLAVLHLQHSKGRDYRDNTELLHHGKHRPEEGPLGRTAMQVRAVKP

Target ORF information:

RefSeq Version	XM_023724504.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023724504.1

ORF Insert Sequence:

ATGGACTCCT TTAGAACAGA TGAAACAGCT GCCAAGCCCA GGTGTAGACC ACATGTCCAC 
TTCCAAATGG CCCTGCTGGC CTGTTCCTTA AGCTCCTTGA ATGCGGGAGG TGGGGGTCAG 
GAGGCAAGCA GTGCCTCGGT CCAGGATTGG TTGACATCAG GGCTAATCTC CAACTCAGAG 
AAGGGTCAGC TGCAAGATTT AGATACCTCC TGCCCGGGTT CCTCTAGCTA TGTCAGCAAT 
TCTTGTCTTT TTTTGGTTAC AAGAAAAATG GATTTTTCTG CACATGTGCT AATGAAGCCT 
TTGATCTCTT TCAGGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGGTAG GCGGGGAAGG 
CAGGAGGCCG GGCAGCTGGC TCAGCAGGGA GGTGCCTTAG GGACCACGTC CTCCAAGGAA 
CATGGTGGAG AAGTGCTGGA TGTTTCTCTC CCCTCGAATG CTTGCATAGA AGAATGCGAC 
GTATCTCCAC CTGAGCTGCC CCCCAGGAAC ATCCCTTTGT CTGCTGGGCC CTGTGAAGCC 
AAGGAAGTTC CTCTTTCAAG CGAGAGTCCC CGAGTGCCCG AGGTCAGCAA GCCGACCCTC 
TCTGAGACGT TACTTCAGAG GCTGCAGAGC ATCGACACTA GTGGGCTTCC GGACGAGTAT 
CTTAAAGCCA TCCAGGATTA TTTAAGCACG CAGCTAGTCC TCGACTCTGA GTTTGTGAAG 
ACGGGCTCCA GCAGTCTCCC TCACCTGAAG AAACTGACCA CGCTCCTGTG CAAGGAGCTT 
TATGGCGAGG TCGTTCGGAC TCTTCCCACT CTGGAGTTTC TGCAGAGGCT GTTCGACCAG 
CAGCTGTCCC CTGGCCTCCG CCCACGTCCT CAGGTGTCTG GTGAGGCCAA TCCCATCAAC 
ATGGTGGCCA AGTTCAGCCA ACTGACAAGT CTGCTGTCCT CTATTGAAGA TAAGGTCAAG 
GCCATGCTGC ATGAGGGTCA TGAGTCTCCT CACCGGTGCT CCATCATCCC TCCGGTCAAC 
TTCGAGGTAA AGTCAGAGTC CTTGGGGATT CCTCAGAAAT TGCATCTCAA GGTCGACGTT 
GAGACTGGGA AACTGATCGT TAAGAAGTCC AAGGATGGTT CTGAGGACAA GTACTACAGC 
CACAAGAAAA TCCTGCAGCT CGTCAAGTCT CAGAAGTTTC TTAACAAGCT GGTGATTGTG 
GTGGAAACGG AGAAGGAGAA GACCGTGCGG AAGGAATATG TCTTTGCTGA CTCTAAAAAG 
AGAGAAGGCT TCTGCCAGCT TCTCCAGCAG ATGAAGAACA AGCACTCGGA GCAGCCTGAG 
CCCGACATGA TCACCATCTT CATCGGCACC TGGAACATGG GTAACGCCCC CCCTCCCAAG 
AAGATCACGT CCTGGTTTCT CTCCAAGGGG CAGGGAAAGA CGCGGGATGA CTCAGCAGAC 
TACATCCCCC ATGACATCTA TGTGATCGGC ACCCAGGAGG ACCCCCTGGG AGAAAAGGAG 
TGGCTGGAGA TCCTCAAGCA CTCCCTACAG GAGATCACCA GCATGACCTT TAAAACGATG 
GCCATCCACA CCCTCTGGAA CATCCGCATC GTGGTGCTGG CCAAGCCGGA GCATGAGAAC 
CGCATCAGCC ACGTCTGCAC GAACCACGTC AAGACGGGCA TCGCCAACAC GCTGGGGAAC 
AAGGGAGCTG TGGGGGTATC CTTCATGTTC AATGGCACCT CCTTGGGATT TGTTAACAGC 
CACTTGACCT CAGGAAGCGA AAAGAAACTC AGGCGAAACC AAAACTATAC GAACATTCTC 
CGGTTTCTGG CCCTGGGAGA CAAGAAACTG AGTCCTTTTA ACATCACTCA CCGTTTCACC 
CATCTCTTCT GGCTTGGGGA TCTCAACTAC CGCTTGGAGC TGCCCATCTC GGAGGCAGAC 
ACCATCATCC AGAAAATCAA GCAGCAGCAG TATGCGGACC TCCTGGCCCA CGACCAGCTG 
CTCATGGAGA GGAAAGAGCA GAAGGTCTTC CTTCATTTCG ACGAGGAAGA AATCACATTC 
GCTCCCACCT ACCGTTTTGA AAGACTGACT CGGGACAAAT ACGCCTACAC TAAGCAGAAA 
GCAACAGGGA TGAAGTACAA CTTGCCCTCC TGGTGTGATC GGGTCCTCTG GAAGTCATAC 
CCGCTGGTCC ACGTGGTGTG TCAGTCCTAC GGCAGTACCA GCGACATTAT GACGAGTGAC 
CACAGCCCTG TCTTTGCCAC GTTTGAGGCA GGAGTCACCT CCCAGTTTGT CTCCAAGAAC 
GGCCCTGGGA CTGTGGACAG CCAAGGGCAA ATTGAGTTTC TCAGCTGCTA CGCCACGTTG 
AAGACCAAGT CCCAGACCAA ATTCTACCTT GAGTTCCACT CCAGCTGCTT GGAGAGCTTT 
GTCAAGAGTC AGGAAGGAGA AAACGAAGAA GGAAGCGAAG GAGAGTTGTT GGTGAAGTTT 
GGCGAGACCC TTCCAAAGCT GAAGCCCATT ATCTCTGACC CGGAATACCT GCTGGACCAG 
CACATCCTGA TCAGCATTAA GTCCTCTGAC AGTGACGAAT CCTACGGCGA GGGCTGCATT 
GCCCTACGGT TAGAGGCCAA GGAAACCCAG TTCCCCATCT ACACGCCTCT CACCCACCAC 
GGAGAGATGA TGGGACATTT CCAGGGGGAG ATTAAGCTGC AGACATCCGA AGGCAAGACG 
AGAGAGAAGC TCTACGACTT CGTGAAGACG GAGCGGGATG AGTCCAGCGG GCCCAAGTCT 
CTGAAGAGCT TCACCAGCCA TGACCCCATG AAGCTGTGGG AACCCACCAA CAGGCCCAGC 
GACTTGGCGA AGAACATAGC AGAGCCCCTG CTTCAGGAGG ACCTGCCGCT GACCAAGCAG 
AAGCCGGAGA TGTTTGAGAA CCCGCTGTAC GGGTCTGTCA GCTCCTTCCC TAAGCTGGTT 
CCCAGGAAAG AGCAGGAATC CCCCAAGATG CTGAGGAAGG AGCCCCCACC CTGCCCAGAC 
CCTGGAGTCT CCTCACCCAC CATCTTGCTC ACCAAAGCGC AGGAGGCCGA GAGCTCCAAG 
GGGACTAGCA AGCAGGTCCC CACGCCCCGG CTGCGCTCCT TCACCTGCTC GTCCTCCACC 
GCAGAGGCCG GAGCGGCGGC CGGCGGGGAG AAGAACCACG GGAAGCCCAA GCCCGCGGCC 
AGCTCCCAGG CGCCGGCGCC CGTGCCGGCG AAGAGGCCCG TGAAGCCGTC CAGGTCAGAA 
ATGAGCCAGC CGATCCCGCC CGCGCCCACG CAGCTCCGAC CGCCCCTGCC CGCCAAAAGC 
CTGGCCGTGC TGCACCTGCA GCACTCCAAG GGCCGCGACT ACCGCGACAA CACCGAGCTC 
TTGCACCACG GCAAGCACCG GCCCGAGGAG GGGCCGCTCG GCAGGACCGC CATGCAGGTG 
CGCGCAGTGA AGCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101350772 ( XM_023724505.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023580273.1 Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X6, mRNA.

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CloneID	OTr227352
Clone ID Related Accession (Same CDS sequence)	XM_023724505.1
Accession Version	XM_023724505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444185.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCCT GCTGGAACCA TGGCAATATC ACGCGCTCTA AGGCCGAGGA GCTGCTCTCC 
AGGACGGGCA AGGATGGGAG CTTTCTCGTG CGTGCCAGTG AGTCCATCTC CCGGGCTTAC 
GCCCTCTGCG TGCTGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGGTGCGCG CAGTGAAGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023580273.1
CDS	81..3674
Translation	MVPCWNHGNITRSKAEELLSRTGKDGSFLVRASESISRAYALCVLYRNCVYTYRILPNEDKFSVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQYPVPLEEEDASDEPEEETECDVSPPELPPRNIPLSAGPCEAKEVPLSSESPRVPEVSKPTLSETLLQRLQSIDTSGLPDEYLKAIQDYLSTQLVLDSEFVKTGSSSLPHLKKLTTLLCKELYGEVVRTLPTLEFLQRLFDQQLSPGLRPRPQVSGEANPINMVAKFSQLTSLLSSIEDKVKAMLHEGHESPHRCSIIPPVNFEVKSESLGIPQKLHLKVDVETGKLIVKKSKDGSEDKYYSHKKILQLVKSQKFLNKLVIVVETEKEKTVRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILKHSLQEITSMTFKTMAIHTLWNIRIVVLAKPEHENRISHVCTNHVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYTNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRLELPISEADTIIQKIKQQQYADLLAHDQLLMERKEQKVFLHFDEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLSCYATLKTKSQTKFYLEFHSSCLESFVKSQEGENEEGSEGELLVKFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLEAKETQFPIYTPLTHHGEMMGHFQGEIKLQTSEGKTREKLYDFVKTERDESSGPKSLKSFTSHDPMKLWEPTNRAPLCSGPSITEIINPNYMGVGPFGPPMPLHGKQVISPDQQPTAWSYDPLPKDASLGPGWGESPPTPPSQQPVSPKKFSPSPASRGPSPRTQESRPSDLAKNIAEPLLQEDLPLTKQKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPDPGVSSPTILLTKAQEAESSKGTSKQVPTPRLRSFTCSSSTAEAGAAAGGEKNHGKPKPAASSQAPAPVPAKRPVKPSRSEMSQPIPPAPTQLRPPLPAKSLAVLHLQHSKGRDYRDNTELLHHGKHRPEEGPLGRTAMQVRAVKP

Target ORF information:

RefSeq Version	XM_023724505.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris inositol polyphosphate-5-phosphatase D (LOC101350772), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023724505.1

ORF Insert Sequence:

ATGGTGCCCT GCTGGAACCA TGGCAATATC ACGCGCTCTA AGGCCGAGGA GCTGCTCTCC 
AGGACGGGCA AGGATGGGAG CTTTCTCGTG CGTGCCAGTG AGTCCATCTC CCGGGCTTAC 
GCCCTCTGCG TGCTGTATCG GAATTGCGTT TACACTTACA GAATTCTGCC CAACGAAGAT 
AAGTTCTCTG TTCAGGCATC CGAGGGCGTC CCCATGAGGT TCTTCACCAA GCTGGACCAG 
CTCATTGAGT TCTACAAGAA GGAGAACATG GGCCTGGTGA CTCACCTGCA GTACCCTGTG 
CCCCTGGAGG AAGAAGATGC CAGTGACGAG CCCGAGGAGG AGACGGAATG CGACGTATCT 
CCACCTGAGC TGCCCCCCAG GAACATCCCT TTGTCTGCTG GGCCCTGTGA AGCCAAGGAA 
GTTCCTCTTT CAAGCGAGAG TCCCCGAGTG CCCGAGGTCA GCAAGCCGAC CCTCTCTGAG 
ACGTTACTTC AGAGGCTGCA GAGCATCGAC ACTAGTGGGC TTCCGGACGA GTATCTTAAA 
GCCATCCAGG ATTATTTAAG CACGCAGCTA GTCCTCGACT CTGAGTTTGT GAAGACGGGC 
TCCAGCAGTC TCCCTCACCT GAAGAAACTG ACCACGCTCC TGTGCAAGGA GCTTTATGGC 
GAGGTCGTTC GGACTCTTCC CACTCTGGAG TTTCTGCAGA GGCTGTTCGA CCAGCAGCTG 
TCCCCTGGCC TCCGCCCACG TCCTCAGGTG TCTGGTGAGG CCAATCCCAT CAACATGGTG 
GCCAAGTTCA GCCAACTGAC AAGTCTGCTG TCCTCTATTG AAGATAAGGT CAAGGCCATG 
CTGCATGAGG GTCATGAGTC TCCTCACCGG TGCTCCATCA TCCCTCCGGT CAACTTCGAG 
GTAAAGTCAG AGTCCTTGGG GATTCCTCAG AAATTGCATC TCAAGGTCGA CGTTGAGACT 
GGGAAACTGA TCGTTAAGAA GTCCAAGGAT GGTTCTGAGG ACAAGTACTA CAGCCACAAG 
AAAATCCTGC AGCTCGTCAA GTCTCAGAAG TTTCTTAACA AGCTGGTGAT TGTGGTGGAA 
ACGGAGAAGG AGAAGACCGT GCGGAAGGAA TATGTCTTTG CTGACTCTAA AAAGAGAGAA 
GGCTTCTGCC AGCTTCTCCA GCAGATGAAG AACAAGCACT CGGAGCAGCC TGAGCCCGAC 
ATGATCACCA TCTTCATCGG CACCTGGAAC ATGGGTAACG CCCCCCCTCC CAAGAAGATC 
ACGTCCTGGT TTCTCTCCAA GGGGCAGGGA AAGACGCGGG ATGACTCAGC AGACTACATC 
CCCCATGACA TCTATGTGAT CGGCACCCAG GAGGACCCCC TGGGAGAAAA GGAGTGGCTG 
GAGATCCTCA AGCACTCCCT ACAGGAGATC ACCAGCATGA CCTTTAAAAC GATGGCCATC 
CACACCCTCT GGAACATCCG CATCGTGGTG CTGGCCAAGC CGGAGCATGA GAACCGCATC 
AGCCACGTCT GCACGAACCA CGTCAAGACG GGCATCGCCA ACACGCTGGG GAACAAGGGA 
GCTGTGGGGG TATCCTTCAT GTTCAATGGC ACCTCCTTGG GATTTGTTAA CAGCCACTTG 
ACCTCAGGAA GCGAAAAGAA ACTCAGGCGA AACCAAAACT ATACGAACAT TCTCCGGTTT 
CTGGCCCTGG GAGACAAGAA ACTGAGTCCT TTTAACATCA CTCACCGTTT CACCCATCTC 
TTCTGGCTTG GGGATCTCAA CTACCGCTTG GAGCTGCCCA TCTCGGAGGC AGACACCATC 
ATCCAGAAAA TCAAGCAGCA GCAGTATGCG GACCTCCTGG CCCACGACCA GCTGCTCATG 
GAGAGGAAAG AGCAGAAGGT CTTCCTTCAT TTCGACGAGG AAGAAATCAC ATTCGCTCCC 
ACCTACCGTT TTGAAAGACT GACTCGGGAC AAATACGCCT ACACTAAGCA GAAAGCAACA 
GGGATGAAGT ACAACTTGCC CTCCTGGTGT GATCGGGTCC TCTGGAAGTC ATACCCGCTG 
GTCCACGTGG TGTGTCAGTC CTACGGCAGT ACCAGCGACA TTATGACGAG TGACCACAGC 
CCTGTCTTTG CCACGTTTGA GGCAGGAGTC ACCTCCCAGT TTGTCTCCAA GAACGGCCCT 
GGGACTGTGG ACAGCCAAGG GCAAATTGAG TTTCTCAGCT GCTACGCCAC GTTGAAGACC 
AAGTCCCAGA CCAAATTCTA CCTTGAGTTC CACTCCAGCT GCTTGGAGAG CTTTGTCAAG 
AGTCAGGAAG GAGAAAACGA AGAAGGAAGC GAAGGAGAGT TGTTGGTGAA GTTTGGCGAG 
ACCCTTCCAA AGCTGAAGCC CATTATCTCT GACCCGGAAT ACCTGCTGGA CCAGCACATC 
CTGATCAGCA TTAAGTCCTC TGACAGTGAC GAATCCTACG GCGAGGGCTG CATTGCCCTA 
CGGTTAGAGG CCAAGGAAAC CCAGTTCCCC ATCTACACGC CTCTCACCCA CCACGGAGAG 
ATGATGGGAC ATTTCCAGGG GGAGATTAAG CTGCAGACAT CCGAAGGCAA GACGAGAGAG 
AAGCTCTACG ACTTCGTGAA GACGGAGCGG GATGAGTCCA GCGGGCCCAA GTCTCTGAAG 
AGCTTCACCA GCCATGACCC CATGAAGCTG TGGGAACCCA CCAACAGGGC CCCTCTGTGC 
AGTGGCCCCA GCATCACCGA GATCATTAAC CCCAACTACA TGGGTGTGGG GCCCTTTGGG 
CCACCGATGC CCCTGCACGG GAAGCAGGTC ATCTCCCCCG ACCAGCAGCC CACAGCCTGG 
AGCTACGACC CACTTCCCAA GGATGCCTCC CTTGGGCCCG GATGGGGGGA GAGTCCTCCA 
ACACCACCCT CCCAACAGCC CGTATCACCC AAGAAGTTTT CACCCTCTCC AGCCAGTCGG 
GGCCCCAGCC CCAGGACACA GGAGTCGAGG CCCAGCGACT TGGCGAAGAA CATAGCAGAG 
CCCCTGCTTC AGGAGGACCT GCCGCTGACC AAGCAGAAGC CGGAGATGTT TGAGAACCCG 
CTGTACGGGT CTGTCAGCTC CTTCCCTAAG CTGGTTCCCA GGAAAGAGCA GGAATCCCCC 
AAGATGCTGA GGAAGGAGCC CCCACCCTGC CCAGACCCTG GAGTCTCCTC ACCCACCATC 
TTGCTCACCA AAGCGCAGGA GGCCGAGAGC TCCAAGGGGA CTAGCAAGCA GGTCCCCACG 
CCCCGGCTGC GCTCCTTCAC CTGCTCGTCC TCCACCGCAG AGGCCGGAGC GGCGGCCGGC 
GGGGAGAAGA ACCACGGGAA GCCCAAGCCC GCGGCCAGCT CCCAGGCGCC GGCGCCCGTG 
CCGGCGAAGA GGCCCGTGAA GCCGTCCAGG TCAGAAATGA GCCAGCCGAT CCCGCCCGCG 
CCCACGCAGC TCCGACCGCC CCTGCCCGCC AAAAGCCTGG CCGTGCTGCA CCTGCAGCAC 
TCCAAGGGCC GCGACTACCG CGACAACACC GAGCTCTTGC ACCACGGCAA GCACCGGCCC 
GAGGAGGGGC CGCTCGGCAG GACCGCCATG CAGGTGCGCG CAGTGAAGCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

LOC101350772 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム