ECE2 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	ECE2
Entrez Gene ID	106553287
Full Name	endothelin converting enzyme 2
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014071758.1	XP_013927233.1	endothelin-converting enzyme 2

Pathways from BioSystems
Gene Ontology

The following ECE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh28598	XM_014071758.1 Latest version!	Thamnophis sirtalis endothelin converting enzyme 2 (ECE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECE2 ( XM_014071758.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013927233.1 Thamnophis sirtalis endothelin converting enzyme 2 (ECE2), mRNA.

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CloneID	OTh28598
Clone ID Related Accession (Same CDS sequence)	XM_014071758.1
Accession Version	XM_014071758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	endothelin-converting enzyme 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013659931.1) and transcript sequence (GDKU01030448.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1158 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAACACC TGTTGGAAAA TGCCACTTTT AACTCCAGCA GTGAGGCTGA AAGGAAGACC 
CAGCGCTATT ACTTGTCCTG CCTCAGAGAA CAGAAGATCG AGGAACTGGG GTCTCAGCCA 
CTGATTGATC TGATCGAGAA GATTGGTGGT TGGAACGTCA CTGGCTCCTG GAACCAGACC 
AACTTCATGG ACGTCCTGAA ATTGGTCTCT GCCACCTATC GCGCTTCTCC GTTCTTCAGT 
GTTTTCGTAG GACCTGATTC TAAAAGCTCC AACAGCAACA TCATCCAGGT GGATCAGTCC 
GGCCTATTTT TACCATCACG AGACTATTAC TTGAACAAGA CCGCCAACAA GAAGGTGTTG 
TCTGTCTACC TGGAGTACAT GGTGGAGCTG GCGATGCTGT TGGGAGGAGC CAGAAGCTCA 
GCGGAAGAAC AGATGGGGCA GGTGCTGCTC CTGGAGACCG AGTTGGCCAA CATCACCGTC 
CCGCAAGACG AACGGCGAGA TGATGAGAAG ATTTACCACA AGATGACCAT TGCTGAGCTG 
CAGGTTCTTG CCCCTGCGAT TGAGTGGCTG GACTTCATCT CATACTTTCT CGCACCACTA 
GAACTAACGG ATGCCGAGCC AGTGGTGGTC TATGGGAAGG AGTATTTGGA GCAGGTGTCA 
CACCTCATCA ATAACACTGA CAAAAGCGTT TTGAACAATT ACATGATTTG GAATCTGGTT 
CAGAAGACAG CTTCCAGCCT CGATCAGCGG TTTGAGACGG CCCAGGAGAA GCTGTTGGAG 
ACTTTGTACG GTACCAAGAA GTCCTGCACA CCTCGCTGGC AAACGTGCAT TTCCAACACG 
GATGACACCT TGGGTTTCGC TCTCGGGGCC CTTTTTGTAA AAGCCACGTT CGACCGACAC 
AGCAAAGAGA TTGCTGAGGA CATGATTGCC GAAATTCGTG CAGCCTTTCA AGAGTCACTA 
AACAATCTTG ACTGGATGGA CAAGAAGACA AAGCAAGCTG CGAAGGAGAA GGCTGATGCC 
ATCTATGACA TGATTGGCTT CCCCGAGTTC ATTTTTGACA ACAAGGAGCT GGATGATGTC 
TACGATGGGT ATGAGGTCTC GGAAGACTCT TTCTTTCAGA ATATGTTGAA TTTCTACAAC 
TTCTCGGCCA GGTTTATGGC AGACCAACTG AGGAAGCCTC CCAACCGGGA CCAGTGGAGC 
ATGACCCCCC AGACCGTCAA CGCTTATTAC CTGCCTACCA AGAACGGCAT CGTCTTTCCT 
GCAGGCATCC TCCAGGCTCC GTTCTACGCC CACAACCATC CCAAGGCCCT CAACTTCGGA 
GGCATCGGCG TTGTGATGGG GCACGAGCTC ACCCATGCTT TCGATGATCA AGGCCGAGAG 
TACGACAGAG AAGGGAACCT GCGTCCTTGG TGGCAAAACT CATCCCTTGA GGCCTTCAAG 
AACAGGACGG AGTGCATGGA GGAACAATAC AGCAAATACC TTGTCAACGG AGAGCACGTC 
AACGGCAAAC AGACTTTGGG AGAGAACATT GCTGACCACG GGGGCCTGAA AGCCGCTTAC 
AACGCTTACA AAGCTTGGCT GAAGAAGAAT GGGGAGGAAA AACACCTGCC CTCCGTGGAT 
CTTACGAGTC ACCAACTCTT CTTTTTAGGA TTCGCACAGG TGTGGTGTTC GGTGAGGACC 
CCAGAGAGCT CGCACGAGGG CCTGATGACG GACCCCCACA GCCCTGACAA GTTCCGGGTC 
ATCGGCACTC TCAGCAACTC CCGGGACTTT TTAGAGCACT TTGAATGCCC GATAGGCTCT 
TTCATGAACC CTGGGAAATA TTGTGAGGTA TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013927233.1
CDS	148..1983
Translation	MKHLLENATFNSSSEAERKTQRYYLSCLREQKIEELGSQPLIDLIEKIGGWNVTGSWNQTNFMDVLKLVSATYRASPFFSVFVGPDSKSSNSNIIQVDQSGLFLPSRDYYLNKTANKKVLSVYLEYMVELAMLLGGARSSAEEQMGQVLLLETELANITVPQDERRDDEKIYHKMTIAELQVLAPAIEWLDFISYFLAPLELTDAEPVVVYGKEYLEQVSHLINNTDKSVLNNYMIWNLVQKTASSLDQRFETAQEKLLETLYGTKKSCTPRWQTCISNTDDTLGFALGALFVKATFDRHSKEIAEDMIAEIRAAFQESLNNLDWMDKKTKQAAKEKADAIYDMIGFPEFIFDNKELDDVYDGYEVSEDSFFQNMLNFYNFSARFMADQLRKPPNRDQWSMTPQTVNAYYLPTKNGIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDREGNLRPWWQNSSLEAFKNRTECMEEQYSKYLVNGEHVNGKQTLGENIADHGGLKAAYNAYKAWLKKNGEEKHLPSVDLTSHQLFFLGFAQVWCSVRTPESSHEGLMTDPHSPDKFRVIGTLSNSRDFLEHFECPIGSFMNPGKYCEVW

Target ORF information:

RefSeq Version	XM_014071758.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis endothelin converting enzyme 2 (ECE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014071758.1

ORF Insert Sequence:

ATGAAACACC TGTTGGAAAA TGCCACTTTT AACTCCAGCA GTGAGGCTGA AAGGAAGACC 
CAGCGCTATT ACTTGTCCTG CCTCAGAGAA CAGAAGATCG AGGAACTGGG GTCTCAGCCA 
CTGATTGATC TGATCGAGAA GATTGGTGGT TGGAACGTCA CTGGCTCCTG GAACCAGACC 
AACTTCATGG ACGTCCTGAA ATTGGTCTCT GCCACCTATC GCGCTTCTCC GTTCTTCAGT 
GTTTTCGTAG GACCTGATTC TAAAAGCTCC AACAGCAACA TCATCCAGGT GGATCAGTCC 
GGCCTATTTT TACCATCACG AGACTATTAC TTGAACAAGA CCGCCAACAA GAAGGTGTTG 
TCTGTCTACC TGGAGTACAT GGTGGAGCTG GCGATGCTGT TGGGAGGAGC CAGAAGCTCA 
GCGGAAGAAC AGATGGGGCA GGTGCTGCTC CTGGAGACCG AGTTGGCCAA CATCACCGTC 
CCGCAAGACG AACGGCGAGA TGATGAGAAG ATTTACCACA AGATGACCAT TGCTGAGCTG 
CAGGTTCTTG CCCCTGCGAT TGAGTGGCTG GACTTCATCT CATACTTTCT CGCACCACTA 
GAACTAACGG ATGCCGAGCC AGTGGTGGTC TATGGGAAGG AGTATTTGGA GCAGGTGTCA 
CACCTCATCA ATAACACTGA CAAAAGCGTT TTGAACAATT ACATGATTTG GAATCTGGTT 
CAGAAGACAG CTTCCAGCCT CGATCAGCGG TTTGAGACGG CCCAGGAGAA GCTGTTGGAG 
ACTTTGTACG GTACCAAGAA GTCCTGCACA CCTCGCTGGC AAACGTGCAT TTCCAACACG 
GATGACACCT TGGGTTTCGC TCTCGGGGCC CTTTTTGTAA AAGCCACGTT CGACCGACAC 
AGCAAAGAGA TTGCTGAGGA CATGATTGCC GAAATTCGTG CAGCCTTTCA AGAGTCACTA 
AACAATCTTG ACTGGATGGA CAAGAAGACA AAGCAAGCTG CGAAGGAGAA GGCTGATGCC 
ATCTATGACA TGATTGGCTT CCCCGAGTTC ATTTTTGACA ACAAGGAGCT GGATGATGTC 
TACGATGGGT ATGAGGTCTC GGAAGACTCT TTCTTTCAGA ATATGTTGAA TTTCTACAAC 
TTCTCGGCCA GGTTTATGGC AGACCAACTG AGGAAGCCTC CCAACCGGGA CCAGTGGAGC 
ATGACCCCCC AGACCGTCAA CGCTTATTAC CTGCCTACCA AGAACGGCAT CGTCTTTCCT 
GCAGGCATCC TCCAGGCTCC GTTCTACGCC CACAACCATC CCAAGGCCCT CAACTTCGGA 
GGCATCGGCG TTGTGATGGG GCACGAGCTC ACCCATGCTT TCGATGATCA AGGCCGAGAG 
TACGACAGAG AAGGGAACCT GCGTCCTTGG TGGCAAAACT CATCCCTTGA GGCCTTCAAG 
AACAGGACGG AGTGCATGGA GGAACAATAC AGCAAATACC TTGTCAACGG AGAGCACGTC 
AACGGCAAAC AGACTTTGGG AGAGAACATT GCTGACCACG GGGGCCTGAA AGCCGCTTAC 
AACGCTTACA AAGCTTGGCT GAAGAAGAAT GGGGAGGAAA AACACCTGCC CTCCGTGGAT 
CTTACGAGTC ACCAACTCTT CTTTTTAGGA TTCGCACAGG TGTGGTGTTC GGTGAGGACC 
CCAGAGAGCT CGCACGAGGG CCTGATGACG GACCCCCACA GCCCTGACAA GTTCCGGGTC 
ATCGGCACTC TCAGCAACTC CCGGGACTTT TTAGAGCACT TTGAATGCCC GATAGGCTCT 
TTCATGAACC CTGGGAAATA TTGTGAGGTA TGGTAG