AMBRA1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	AMBRA1
Entrez Gene ID	106552302
Full Name	autophagy and beclin 1 regulator 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014070546.1	XP_013926021.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_014070560.1	XP_013926035.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_014070567.1	XP_013926042.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_014070575.1	XP_013926050.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_014070582.1	XP_013926057.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_014070554.1	XP_013926029.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following AMBRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMBRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTh12161	XM_014070582.1 Latest version!	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTh12159	XM_014070554.1 Latest version!	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTh12159	XM_014070546.1 Latest version!	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTh12159	XM_014070560.1 Latest version!	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTh12159	XM_014070567.1 Latest version!	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTh12160	XM_014070575.1 Latest version!	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AMBRA1 ( XM_014070582.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013926057.1 Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OTh12161
Clone ID Related Accession (Same CDS sequence)	XM_014070582.1
Accession Version	XM_014070582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657740.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGACCCC ACATAGCAGT 
GAAAACAATT CCAATTTGTC ACGGGGGCAT CTGAACCGCT GTCGCGCCTG TCACAACCTG 
CTGACTTTTA ACAATGACAC ACTTCGCTGG GAAAGAAGCA CACCCAGCTA TGCACCAGGG 
CAGTCCCAAA GCACATTTGA GGGGGTGCCA TCCAGCAGTA GCCAGCTACC ACCACCTGAG 
AGACTGGAAA GCCGAACTTC TCCCTCTAGC AGGCTGCCCC TGAGAAGATC TTCTAATCCT 
CAAGAGGAAA GGACTGTGAG AGGGGTCTTT AATCAAGAGA CGGGGCACTG GGAAAGAATT 
TACAGCCAAT CTGCTTCAAA TAGACCTGAG AATGTATCAC AGGACGCCTT AAACCAGGAA 
ATGCCTGAGG AAATTTCAGA GGAGGATTCA CTCAGGAGGA GGCTGTTGGA ATCTTCACTC 
ATTTCACTCT CACGTTATGA TGGGGCAGGA TCCCGCGAAC ATCCTATATA TCCTGATCCA 
GCTAGGTTGT CACCTGCTGC TTATTATGCC CAGAGAATGA TCCAGTATCT TTCACGCAGA 
GATAGCATTC GCCAGCGCTC CATGCGGTAT CAGCAAAATC GCCTCCGCTC TTCCTCATCT 
TCTGCTTCCA CTTCTGAGAA TGCAGGTCCA TCTGTAGAAG GAAATGACCT GGAATTTGAA 
GACTTCGAGG ACAATGGAGA CAGGTCAAGA CACCGAGCCC CTAGGAATGC CCGTATGTCT 
GCACCATCCC TAGGACGCTT CGTCCCAAGA CGCTTCTTGT TGCCTGAGTA TTTGCCTTAC 
GCTGGGATTT TCCATGAGCG TGGACAGCCT GGGTTGGCCA CTCACTCCTC TGTCAATAGA 
GTTTTAGCAG GGGCTGTAAT TGGAGATGGA CAGTCTGCTG TGGCCAGCAA TATTGCAAAC 
ACCACATACC GGCTCCAGTG GTGGGATTTC ACTAAATTCG ACTTACCAGA AATAAGCAAT 
GCATCTGTGA ATGTGCTTGT TCAGAACTGC AAGATATACA ATGATGCCAG CTGTGATATT 
TCTGCAGATG GGCAGCTTTT AGCAGCTTTC ATTCCTAGCA GTCAGCGAGG ATTTCCTGAT 
GAGGGGATAC TCGCAGTCTA TTCCCTTGCC CCACACAACT TGGGTGAAAT GCTGTATACG 
AAGCGATTTG GTCCCAATGC CATTTCTGTG AGCTTGTCCC CAATGGGCAG GTATGTCATG 
GTAGGATTGG CTTCTCGTCG CATCTTGTTG CACCCATCTA CAGAACATAT GGTGGCTCAG 
GTTTTCAGGC TTCAGCAACC ACATGGAGGA GAGACTTCAA TGAGGAGAGT GTTCAATGTC 
TTGTACCCAA TGCCTGCCGA TCAAAGGAGA CACGTCAGCA TAAATTCTGC TCGCTGGTTA 
CCTGAGCCGG GTTTAGGATT AGCTTATGGC ACTAATAAAG GGGATCTGGT GATTTGCCGA 
CCAGAGGCGT TAAACTCTGG TGTAGAATAC TACTGGGACC AGCTGAATGA AACTGTCTTC 
ACTGTGCATT CCAGCAGCAG AAGCACTGAA CGCCCAGGAA CCAGCAGAGC CACCTGGAGG 
ACGGACCGGG ATTTGGGCTT AATGAATGCC ATTGGCCTGC AGCCACGGAA CCCAACCACC 
TCTGTGACTT CGCAGGGCAC ACAGACTCTG GCACCTCAGC TACAGAATGC AGAAACACAA 
ACCGAGAGAG AAGTTCAGGA ACCAGGAAGT CTTACTTCAG GGGTTACTGA AGGTGGTTCA 
GATTATGGAG CAACTGGAGA AGATGCCCTA ATTAGGATCC AAAGGCTGAT GGCTGAAGGT 
GGCATGACTG CTGTCGTCCA ACGAGAGCAA AGCACTACCA TGGCATCCAT GGGTGGTTTT 
GGCAACAACA TAATTGTGAG TCACAGGATC CACCGTAGCT CTCAGACTGG CACTGAATCC 
ACTGGCTTAG AAGGAGGCTC AGCTCAACCA TCGACTTCGC AGGAGCTGAT AACAGAGCTA 
GAAGGACGGA CCCTTTCTGA GTCTATGCAG GTGACAGAAC GTGGCCTGAG CCCACAGACA 
TCGTCTAGTG AGGTTGAGGG AGAAGCTAAT GGGCAAAGCC TACCAGAGCA AGCTCATTCC 
TCAATGGACA CTGAGGGACC AGTCGAGTAT AGTGATCTAA CTAATAATAA CCATCTTCCT 
GACAATACCA ATTTCTATAG TAATGAGAGC ACCAATGGAG AGTCTCGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013926057.1
CDS	299..3295
Translation	MKVVPEKNAVRILWGRERGTQALGAQRLLQELVEDKTRWMKWEGKKVELPDSPRSTFLLAFSPDRTLMASTHVNHNIYITEVKTGRCVHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQNDEEVEIPVDSTEIPHYRQRSILQSQPVRRTPLLHNFLHMLSSRSSGIQTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPSYAPGQSQSTFEGVPSSSSQLPPPERLESRTSPSSRLPLRRSSNPQEERTVRGVFNQETGHWERIYSQSASNRPENVSQDALNQEMPEEISEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSSSSASTSENAGPSVEGNDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSSSRSTERPGTSRATWRTDRDLGLMNAIGLQPRNPTTSVTSQGTQTLAPQLQNAETQTEREVQEPGSLTSGVTEGGSDYGATGEDALIRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESTGLEGGSAQPSTSQELITELEGRTLSESMQVTERGLSPQTSSSEVEGEANGQSLPEQAHSSMDTEGPVEYSDLTNNNHLPDNTNFYSNESTNGESRNR

Target ORF information:

RefSeq Version	XM_014070582.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014070582.1

ORF Insert Sequence:

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGACCCC ACATAGCAGT 
GAAAACAATT CCAATTTGTC ACGGGGGCAT CTGAACCGCT GTCGCGCCTG TCACAACCTG 
CTGACTTTTA ACAATGACAC ACTTCGCTGG GAAAGAAGCA CACCCAGCTA TGCACCAGGG 
CAGTCCCAAA GCACATTTGA GGGGGTGCCA TCCAGCAGTA GCCAGCTACC ACCACCTGAG 
AGACTGGAAA GCCGAACTTC TCCCTCTAGC AGGCTGCCCC TGAGAAGATC TTCTAATCCT 
CAAGAGGAAA GGACTGTGAG AGGGGTCTTT AATCAAGAGA CGGGGCACTG GGAAAGAATT 
TACAGCCAAT CTGCTTCAAA TAGACCTGAG AATGTATCAC AGGACGCCTT AAACCAGGAA 
ATGCCTGAGG AAATTTCAGA GGAGGATTCA CTCAGGAGGA GGCTGTTGGA ATCTTCACTC 
ATTTCACTCT CACGTTATGA TGGGGCAGGA TCCCGCGAAC ATCCTATATA TCCTGATCCA 
GCTAGGTTGT CACCTGCTGC TTATTATGCC CAGAGAATGA TCCAGTATCT TTCACGCAGA 
GATAGCATTC GCCAGCGCTC CATGCGGTAT CAGCAAAATC GCCTCCGCTC TTCCTCATCT 
TCTGCTTCCA CTTCTGAGAA TGCAGGTCCA TCTGTAGAAG GAAATGACCT GGAATTTGAA 
GACTTCGAGG ACAATGGAGA CAGGTCAAGA CACCGAGCCC CTAGGAATGC CCGTATGTCT 
GCACCATCCC TAGGACGCTT CGTCCCAAGA CGCTTCTTGT TGCCTGAGTA TTTGCCTTAC 
GCTGGGATTT TCCATGAGCG TGGACAGCCT GGGTTGGCCA CTCACTCCTC TGTCAATAGA 
GTTTTAGCAG GGGCTGTAAT TGGAGATGGA CAGTCTGCTG TGGCCAGCAA TATTGCAAAC 
ACCACATACC GGCTCCAGTG GTGGGATTTC ACTAAATTCG ACTTACCAGA AATAAGCAAT 
GCATCTGTGA ATGTGCTTGT TCAGAACTGC AAGATATACA ATGATGCCAG CTGTGATATT 
TCTGCAGATG GGCAGCTTTT AGCAGCTTTC ATTCCTAGCA GTCAGCGAGG ATTTCCTGAT 
GAGGGGATAC TCGCAGTCTA TTCCCTTGCC CCACACAACT TGGGTGAAAT GCTGTATACG 
AAGCGATTTG GTCCCAATGC CATTTCTGTG AGCTTGTCCC CAATGGGCAG GTATGTCATG 
GTAGGATTGG CTTCTCGTCG CATCTTGTTG CACCCATCTA CAGAACATAT GGTGGCTCAG 
GTTTTCAGGC TTCAGCAACC ACATGGAGGA GAGACTTCAA TGAGGAGAGT GTTCAATGTC 
TTGTACCCAA TGCCTGCCGA TCAAAGGAGA CACGTCAGCA TAAATTCTGC TCGCTGGTTA 
CCTGAGCCGG GTTTAGGATT AGCTTATGGC ACTAATAAAG GGGATCTGGT GATTTGCCGA 
CCAGAGGCGT TAAACTCTGG TGTAGAATAC TACTGGGACC AGCTGAATGA AACTGTCTTC 
ACTGTGCATT CCAGCAGCAG AAGCACTGAA CGCCCAGGAA CCAGCAGAGC CACCTGGAGG 
ACGGACCGGG ATTTGGGCTT AATGAATGCC ATTGGCCTGC AGCCACGGAA CCCAACCACC 
TCTGTGACTT CGCAGGGCAC ACAGACTCTG GCACCTCAGC TACAGAATGC AGAAACACAA 
ACCGAGAGAG AAGTTCAGGA ACCAGGAAGT CTTACTTCAG GGGTTACTGA AGGTGGTTCA 
GATTATGGAG CAACTGGAGA AGATGCCCTA ATTAGGATCC AAAGGCTGAT GGCTGAAGGT 
GGCATGACTG CTGTCGTCCA ACGAGAGCAA AGCACTACCA TGGCATCCAT GGGTGGTTTT 
GGCAACAACA TAATTGTGAG TCACAGGATC CACCGTAGCT CTCAGACTGG CACTGAATCC 
ACTGGCTTAG AAGGAGGCTC AGCTCAACCA TCGACTTCGC AGGAGCTGAT AACAGAGCTA 
GAAGGACGGA CCCTTTCTGA GTCTATGCAG GTGACAGAAC GTGGCCTGAG CCCACAGACA 
TCGTCTAGTG AGGTTGAGGG AGAAGCTAAT GGGCAAAGCC TACCAGAGCA AGCTCATTCC 
TCAATGGACA CTGAGGGACC AGTCGAGTAT AGTGATCTAA CTAATAATAA CCATCTTCCT 
GACAATACCA ATTTCTATAG TAATGAGAGC ACCAATGGAG AGTCTCGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_014070546.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013926021.1 Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTh12159
Clone ID Related Accession (Same CDS sequence)	XM_014070546.1 , XM_014070560.1 , XM_014070567.1 , XM_014070554.1
Accession Version	XM_014070546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657740.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013926021.1
CDS	301..4185
Translation	MKVVPEKNAVRILWGRERGTQALGAQRLLQELVEDKTRWMKWEGKKVELPDSPRSTFLLAFSPDRTLMASTHVNHNIYITEVKTGRCVHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQNDEEVEIPVDSTEIPHYRQRSILQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQNAMQDSATPSPPPPPPPLPPASETSRASAYNGLNEPVNYPSVKCCQHLGFLCLCRRCSSARLPSSLFPSQDNAPSTSSGSTGTSFSSVQMEPYQPQEQASSAQQEQGLLNRPSAFSTVQSSTAGNTLRNFSLGPTRRSLTGSLSGHPSRIHPRQMASGLEGSEWTRTVLNMGPRSELEGMPPPRTSASSVSLLSVLRQQEGSSQSSVYTSATEGRGFPAPEAEAESTSSTGPSNPASLRNELQCDLRRFFLEYDRLQELDQGISGEPSQSHQAQEMLNNNIEPDRPGPSHQQTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPSYAPGQSQSTFEGVPSSSSQLPPPERLESRTSPSSRLPLRRSSNPQEERTVRGVFNQETGHWERIYSQSASNRPENVSQDALNQEMPEEISEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSSSSASTSENAGPSVEGNDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSSSRSTERPGTSRATWRTDRDLGLMNAIGLQPRNPTTSVTSQGTQTLAPQLQNAETQTEREVQEPGSLTSGVTEGGSDYGATGEDALIRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESTGLEGGSAQPSTSQELITELEGRTLSESMQVTERGLSPQTSSSEVEGEANGQSLPEQAHSSMDTEGPVEYSDLTNNNHLPDNTNFYSNESTNGESRNR

Target ORF information:

RefSeq Version	XM_014070546.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014070546.1

ORF Insert Sequence:

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_014070560.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013926035.1 Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTh12159
Clone ID Related Accession (Same CDS sequence)	XM_014070546.1 , XM_014070560.1 , XM_014070567.1 , XM_014070554.1
Accession Version	XM_014070560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657740.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013926035.1
CDS	329..4213
Translation	MKVVPEKNAVRILWGRERGTQALGAQRLLQELVEDKTRWMKWEGKKVELPDSPRSTFLLAFSPDRTLMASTHVNHNIYITEVKTGRCVHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQNDEEVEIPVDSTEIPHYRQRSILQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQNAMQDSATPSPPPPPPPLPPASETSRASAYNGLNEPVNYPSVKCCQHLGFLCLCRRCSSARLPSSLFPSQDNAPSTSSGSTGTSFSSVQMEPYQPQEQASSAQQEQGLLNRPSAFSTVQSSTAGNTLRNFSLGPTRRSLTGSLSGHPSRIHPRQMASGLEGSEWTRTVLNMGPRSELEGMPPPRTSASSVSLLSVLRQQEGSSQSSVYTSATEGRGFPAPEAEAESTSSTGPSNPASLRNELQCDLRRFFLEYDRLQELDQGISGEPSQSHQAQEMLNNNIEPDRPGPSHQQTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPSYAPGQSQSTFEGVPSSSSQLPPPERLESRTSPSSRLPLRRSSNPQEERTVRGVFNQETGHWERIYSQSASNRPENVSQDALNQEMPEEISEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSSSSASTSENAGPSVEGNDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSSSRSTERPGTSRATWRTDRDLGLMNAIGLQPRNPTTSVTSQGTQTLAPQLQNAETQTEREVQEPGSLTSGVTEGGSDYGATGEDALIRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESTGLEGGSAQPSTSQELITELEGRTLSESMQVTERGLSPQTSSSEVEGEANGQSLPEQAHSSMDTEGPVEYSDLTNNNHLPDNTNFYSNESTNGESRNR

Target ORF information:

RefSeq Version	XM_014070560.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014070560.1

ORF Insert Sequence:

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_014070567.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013926042.1 Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTh12159
Clone ID Related Accession (Same CDS sequence)	XM_014070546.1 , XM_014070560.1 , XM_014070567.1 , XM_014070554.1
Accession Version	XM_014070567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657740.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013926042.1
CDS	122..4006
Translation	MKVVPEKNAVRILWGRERGTQALGAQRLLQELVEDKTRWMKWEGKKVELPDSPRSTFLLAFSPDRTLMASTHVNHNIYITEVKTGRCVHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQNDEEVEIPVDSTEIPHYRQRSILQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQNAMQDSATPSPPPPPPPLPPASETSRASAYNGLNEPVNYPSVKCCQHLGFLCLCRRCSSARLPSSLFPSQDNAPSTSSGSTGTSFSSVQMEPYQPQEQASSAQQEQGLLNRPSAFSTVQSSTAGNTLRNFSLGPTRRSLTGSLSGHPSRIHPRQMASGLEGSEWTRTVLNMGPRSELEGMPPPRTSASSVSLLSVLRQQEGSSQSSVYTSATEGRGFPAPEAEAESTSSTGPSNPASLRNELQCDLRRFFLEYDRLQELDQGISGEPSQSHQAQEMLNNNIEPDRPGPSHQQTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPSYAPGQSQSTFEGVPSSSSQLPPPERLESRTSPSSRLPLRRSSNPQEERTVRGVFNQETGHWERIYSQSASNRPENVSQDALNQEMPEEISEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSSSSASTSENAGPSVEGNDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSSSRSTERPGTSRATWRTDRDLGLMNAIGLQPRNPTTSVTSQGTQTLAPQLQNAETQTEREVQEPGSLTSGVTEGGSDYGATGEDALIRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESTGLEGGSAQPSTSQELITELEGRTLSESMQVTERGLSPQTSSSEVEGEANGQSLPEQAHSSMDTEGPVEYSDLTNNNHLPDNTNFYSNESTNGESRNR

Target ORF information:

RefSeq Version	XM_014070567.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014070567.1

ORF Insert Sequence:

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_014070554.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013926029.1 Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTh12159
Clone ID Related Accession (Same CDS sequence)	XM_014070546.1 , XM_014070560.1 , XM_014070567.1 , XM_014070554.1
Accession Version	XM_014070554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657740.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013926029.1
CDS	588..4472
Translation	MKVVPEKNAVRILWGRERGTQALGAQRLLQELVEDKTRWMKWEGKKVELPDSPRSTFLLAFSPDRTLMASTHVNHNIYITEVKTGRCVHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQNDEEVEIPVDSTEIPHYRQRSILQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQNAMQDSATPSPPPPPPPLPPASETSRASAYNGLNEPVNYPSVKCCQHLGFLCLCRRCSSARLPSSLFPSQDNAPSTSSGSTGTSFSSVQMEPYQPQEQASSAQQEQGLLNRPSAFSTVQSSTAGNTLRNFSLGPTRRSLTGSLSGHPSRIHPRQMASGLEGSEWTRTVLNMGPRSELEGMPPPRTSASSVSLLSVLRQQEGSSQSSVYTSATEGRGFPAPEAEAESTSSTGPSNPASLRNELQCDLRRFFLEYDRLQELDQGISGEPSQSHQAQEMLNNNIEPDRPGPSHQQTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPSYAPGQSQSTFEGVPSSSSQLPPPERLESRTSPSSRLPLRRSSNPQEERTVRGVFNQETGHWERIYSQSASNRPENVSQDALNQEMPEEISEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSSSSASTSENAGPSVEGNDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSSSRSTERPGTSRATWRTDRDLGLMNAIGLQPRNPTTSVTSQGTQTLAPQLQNAETQTEREVQEPGSLTSGVTEGGSDYGATGEDALIRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESTGLEGGSAQPSTSQELITELEGRTLSESMQVTERGLSPQTSSSEVEGEANGQSLPEQAHSSMDTEGPVEYSDLTNNNHLPDNTNFYSNESTNGESRNR

Target ORF information:

RefSeq Version	XM_014070554.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014070554.1

ORF Insert Sequence:

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGTGGG AGAGCAAAAC 
GCCATGCAAG ACTCTGCTAC TCCCTCCCCT CCTCCTCCTC CTCCCCCACT TCCTCCAGCT 
AGTGAAACCT CTAGAGCATC TGCCTATAAC GGCCTCAATG AGCCTGTCAA TTACCCCTCA 
GTAAAATGTT GCCAGCATCT GGGGTTTTTG TGCCTTTGCA GGCGATGTTC TAGTGCAAGA 
CTTCCATCTT CTCTCTTCCC ATCCCAGGAC AACGCCCCTT CCACATCCTC TGGGTCCACT 
GGCACTTCCT TTTCTTCTGT GCAAATGGAA CCTTACCAAC CCCAGGAGCA GGCATCTTCA 
GCACAGCAGG AGCAGGGACT CCTTAATCGA CCATCTGCTT TCAGCACTGT TCAGAGCAGT 
ACTGCTGGCA ACACCCTGCG CAACTTTAGC CTGGGCCCCA CTCGTAGATC CCTTACTGGG 
TCTTTGTCAG GCCATCCGTC CAGGATCCAC CCAAGACAGA TGGCCTCAGG GTTGGAGGGT 
TCTGAGTGGA CTCGAACAGT GTTGAATATG GGCCCTCGCT CTGAGTTGGA AGGTATGCCT 
CCTCCTAGAA CCAGTGCCTC TTCAGTGAGT TTGTTGTCGG TGCTGAGACA GCAGGAAGGA 
AGTTCCCAGT CCTCTGTATA TACTTCAGCT ACAGAAGGGA GGGGTTTCCC AGCTCCTGAA 
GCTGAAGCAG AAAGCACCAG TAGTACTGGC CCAAGCAATC CAGCCAGCCT TCGCAATGAG 
CTTCAGTGTG ACTTGAGGCG GTTCTTCTTG GAATATGATA GGCTTCAAGA ACTAGATCAG 
GGGATAAGTG GTGAACCTAG TCAGTCACAT CAGGCCCAGG AAATGCTTAA TAACAACATT 
GAGCCTGATA GGCCTGGTCC TTCTCATCAA CAGACCCCAC ATAGCAGTGA AAACAATTCC 
AATTTGTCAC GGGGGCATCT GAACCGCTGT CGCGCCTGTC ACAACCTGCT GACTTTTAAC 
AATGACACAC TTCGCTGGGA AAGAAGCACA CCCAGCTATG CACCAGGGCA GTCCCAAAGC 
ACATTTGAGG GGGTGCCATC CAGCAGTAGC CAGCTACCAC CACCTGAGAG ACTGGAAAGC 
CGAACTTCTC CCTCTAGCAG GCTGCCCCTG AGAAGATCTT CTAATCCTCA AGAGGAAAGG 
ACTGTGAGAG GGGTCTTTAA TCAAGAGACG GGGCACTGGG AAAGAATTTA CAGCCAATCT 
GCTTCAAATA GACCTGAGAA TGTATCACAG GACGCCTTAA ACCAGGAAAT GCCTGAGGAA 
ATTTCAGAGG AGGATTCACT CAGGAGGAGG CTGTTGGAAT CTTCACTCAT TTCACTCTCA 
CGTTATGATG GGGCAGGATC CCGCGAACAT CCTATATATC CTGATCCAGC TAGGTTGTCA 
CCTGCTGCTT ATTATGCCCA GAGAATGATC CAGTATCTTT CACGCAGAGA TAGCATTCGC 
CAGCGCTCCA TGCGGTATCA GCAAAATCGC CTCCGCTCTT CCTCATCTTC TGCTTCCACT 
TCTGAGAATG CAGGTCCATC TGTAGAAGGA AATGACCTGG AATTTGAAGA CTTCGAGGAC 
AATGGAGACA GGTCAAGACA CCGAGCCCCT AGGAATGCCC GTATGTCTGC ACCATCCCTA 
GGACGCTTCG TCCCAAGACG CTTCTTGTTG CCTGAGTATT TGCCTTACGC TGGGATTTTC 
CATGAGCGTG GACAGCCTGG GTTGGCCACT CACTCCTCTG TCAATAGAGT TTTAGCAGGG 
GCTGTAATTG GAGATGGACA GTCTGCTGTG GCCAGCAATA TTGCAAACAC CACATACCGG 
CTCCAGTGGT GGGATTTCAC TAAATTCGAC TTACCAGAAA TAAGCAATGC ATCTGTGAAT 
GTGCTTGTTC AGAACTGCAA GATATACAAT GATGCCAGCT GTGATATTTC TGCAGATGGG 
CAGCTTTTAG CAGCTTTCAT TCCTAGCAGT CAGCGAGGAT TTCCTGATGA GGGGATACTC 
GCAGTCTATT CCCTTGCCCC ACACAACTTG GGTGAAATGC TGTATACGAA GCGATTTGGT 
CCCAATGCCA TTTCTGTGAG CTTGTCCCCA ATGGGCAGGT ATGTCATGGT AGGATTGGCT 
TCTCGTCGCA TCTTGTTGCA CCCATCTACA GAACATATGG TGGCTCAGGT TTTCAGGCTT 
CAGCAACCAC ATGGAGGAGA GACTTCAATG AGGAGAGTGT TCAATGTCTT GTACCCAATG 
CCTGCCGATC AAAGGAGACA CGTCAGCATA AATTCTGCTC GCTGGTTACC TGAGCCGGGT 
TTAGGATTAG CTTATGGCAC TAATAAAGGG GATCTGGTGA TTTGCCGACC AGAGGCGTTA 
AACTCTGGTG TAGAATACTA CTGGGACCAG CTGAATGAAA CTGTCTTCAC TGTGCATTCC 
AGCAGCAGAA GCACTGAACG CCCAGGAACC AGCAGAGCCA CCTGGAGGAC GGACCGGGAT 
TTGGGCTTAA TGAATGCCAT TGGCCTGCAG CCACGGAACC CAACCACCTC TGTGACTTCG 
CAGGGCACAC AGACTCTGGC ACCTCAGCTA CAGAATGCAG AAACACAAAC CGAGAGAGAA 
GTTCAGGAAC CAGGAAGTCT TACTTCAGGG GTTACTGAAG GTGGTTCAGA TTATGGAGCA 
ACTGGAGAAG ATGCCCTAAT TAGGATCCAA AGGCTGATGG CTGAAGGTGG CATGACTGCT 
GTCGTCCAAC GAGAGCAAAG CACTACCATG GCATCCATGG GTGGTTTTGG CAACAACATA 
ATTGTGAGTC ACAGGATCCA CCGTAGCTCT CAGACTGGCA CTGAATCCAC TGGCTTAGAA 
GGAGGCTCAG CTCAACCATC GACTTCGCAG GAGCTGATAA CAGAGCTAGA AGGACGGACC 
CTTTCTGAGT CTATGCAGGT GACAGAACGT GGCCTGAGCC CACAGACATC GTCTAGTGAG 
GTTGAGGGAG AAGCTAATGG GCAAAGCCTA CCAGAGCAAG CTCATTCCTC AATGGACACT 
GAGGGACCAG TCGAGTATAG TGATCTAACT AATAATAACC ATCTTCCTGA CAATACCAAT 
TTCTATAGTA ATGAGAGCAC CAATGGAGAG TCTCGGAACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_014070575.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013926050.1 Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTh12160
Clone ID Related Accession (Same CDS sequence)	XM_014070575.1
Accession Version	XM_014070575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013657740.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGACAA CGCCCCTTCC 
ACATCCTCTG GGTCCACTGG CACTTCCTTT TCTTCTGTGC AAATGGAACC TTACCAACCC 
CAGGAGCAGG CATCTTCAGC ACAGCAGGAG CAGGGACTCC TTAATCGACC ATCTGCTTTC 
AGCACTGTTC AGAGCAGTAC TGCTGGCAAC ACCCTGCGCA ACTTTAGCCT GGGCCCCACT 
CGTAGATCCC TTACTGGGTC TTTGTCAGGC CATCCGTCCA GGATCCACCC AAGACAGATG 
GCCTCAGGGT TGGAGGGTTC TGAGTGGACT CGAACAGTGT TGAATATGGG CCCTCGCTCT 
GAGTTGGAAG GTATGCCTCC TCCTAGAACC AGTGCCTCTT CAGTGAGTTT GTTGTCGGTG 
CTGAGACAGC AGGAAGGAAG TTCCCAGTCC TCTGTATATA CTTCAGCTAC AGAAGGGAGG 
GGTTTCCCAG CTCCTGAAGC TGAAGCAGAA AGCACCAGTA GTACTGGCCC AAGCAATCCA 
GCCAGCCTTC GCAATGAGCT TCAGTGTGAC TTGAGGCGGT TCTTCTTGGA ATATGATAGG 
CTTCAAGAAC TAGATCAGGG GATAAGTGGT GAACCTAGTC AGTCACATCA GGCCCAGGAA 
ATGCTTAATA ACAACATTGA GCCTGATAGG CCTGGTCCTT CTCATCAACA GACCCCACAT 
AGCAGTGAAA ACAATTCCAA TTTGTCACGG GGGCATCTGA ACCGCTGTCG CGCCTGTCAC 
AACCTGCTGA CTTTTAACAA TGACACACTT CGCTGGGAAA GAAGCACACC CAGCTATGCA 
CCAGGGCAGT CCCAAAGCAC ATTTGAGGGG GTGCCATCCA GCAGTAGCCA GCTACCACCA 
CCTGAGAGAC TGGAAAGCCG AACTTCTCCC TCTAGCAGGC TGCCCCTGAG AAGATCTTCT 
AATCCTCAAG AGGAAAGGAC TGTGAGAGGG GTCTTTAATC AAGAGACGGG GCACTGGGAA 
AGAATTTACA GCCAATCTGC TTCAAATAGA CCTGAGAATG TATCACAGGA CGCCTTAAAC 
CAGGAAATGC CTGAGGAAAT TTCAGAGGAG GATTCACTCA GGAGGAGGCT GTTGGAATCT 
TCACTCATTT CACTCTCACG TTATGATGGG GCAGGATCCC GCGAACATCC TATATATCCT 
GATCCAGCTA GGTTGTCACC TGCTGCTTAT TATGCCCAGA GAATGATCCA GTATCTTTCA 
CGCAGAGATA GCATTCGCCA GCGCTCCATG CGGTATCAGC AAAATCGCCT CCGCTCTTCC 
TCATCTTCTG CTTCCACTTC TGAGAATGCA GGTCCATCTG TAGAAGGAAA TGACCTGGAA 
TTTGAAGACT TCGAGGACAA TGGAGACAGG TCAAGACACC GAGCCCCTAG GAATGCCCGT 
ATGTCTGCAC CATCCCTAGG ACGCTTCGTC CCAAGACGCT TCTTGTTGCC TGAGTATTTG 
CCTTACGCTG GGATTTTCCA TGAGCGTGGA CAGCCTGGGT TGGCCACTCA CTCCTCTGTC 
AATAGAGTTT TAGCAGGGGC TGTAATTGGA GATGGACAGT CTGCTGTGGC CAGCAATATT 
GCAAACACCA CATACCGGCT CCAGTGGTGG GATTTCACTA AATTCGACTT ACCAGAAATA 
AGCAATGCAT CTGTGAATGT GCTTGTTCAG AACTGCAAGA TATACAATGA TGCCAGCTGT 
GATATTTCTG CAGATGGGCA GCTTTTAGCA GCTTTCATTC CTAGCAGTCA GCGAGGATTT 
CCTGATGAGG GGATACTCGC AGTCTATTCC CTTGCCCCAC ACAACTTGGG TGAAATGCTG 
TATACGAAGC GATTTGGTCC CAATGCCATT TCTGTGAGCT TGTCCCCAAT GGGCAGGTAT 
GTCATGGTAG GATTGGCTTC TCGTCGCATC TTGTTGCACC CATCTACAGA ACATATGGTG 
GCTCAGGTTT TCAGGCTTCA GCAACCACAT GGAGGAGAGA CTTCAATGAG GAGAGTGTTC 
AATGTCTTGT ACCCAATGCC TGCCGATCAA AGGAGACACG TCAGCATAAA TTCTGCTCGC 
TGGTTACCTG AGCCGGGTTT AGGATTAGCT TATGGCACTA ATAAAGGGGA TCTGGTGATT 
TGCCGACCAG AGGCGTTAAA CTCTGGTGTA GAATACTACT GGGACCAGCT GAATGAAACT 
GTCTTCACTG TGCATTCCAG CAGCAGAAGC ACTGAACGCC CAGGAACCAG CAGAGCCACC 
TGGAGGACGG ACCGGGATTT GGGCTTAATG AATGCCATTG GCCTGCAGCC ACGGAACCCA 
ACCACCTCTG TGACTTCGCA GGGCACACAG ACTCTGGCAC CTCAGCTACA GAATGCAGAA 
ACACAAACCG AGAGAGAAGT TCAGGAACCA GGAAGTCTTA CTTCAGGGGT TACTGAAGGT 
GGTTCAGATT ATGGAGCAAC TGGAGAAGAT GCCCTAATTA GGATCCAAAG GCTGATGGCT 
GAAGGTGGCA TGACTGCTGT CGTCCAACGA GAGCAAAGCA CTACCATGGC ATCCATGGGT 
GGTTTTGGCA ACAACATAAT TGTGAGTCAC AGGATCCACC GTAGCTCTCA GACTGGCACT 
GAATCCACTG GCTTAGAAGG AGGCTCAGCT CAACCATCGA CTTCGCAGGA GCTGATAACA 
GAGCTAGAAG GACGGACCCT TTCTGAGTCT ATGCAGGTGA CAGAACGTGG CCTGAGCCCA 
CAGACATCGT CTAGTGAGGT TGAGGGAGAA GCTAATGGGC AAAGCCTACC AGAGCAAGCT 
CATTCCTCAA TGGACACTGA GGGACCAGTC GAGTATAGTG ATCTAACTAA TAATAACCAT 
CTTCCTGACA ATACCAATTT CTATAGTAAT GAGAGCACCA ATGGAGAGTC TCGGAACAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013926050.1
CDS	300..3962
Translation	MKVVPEKNAVRILWGRERGTQALGAQRLLQELVEDKTRWMKWEGKKVELPDSPRSTFLLAFSPDRTLMASTHVNHNIYITEVKTGRCVHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQNDEEVEIPVDSTEIPHYRQRSILQSQPVRRTPLLHNFLHMLSSRSSGIQDNAPSTSSGSTGTSFSSVQMEPYQPQEQASSAQQEQGLLNRPSAFSTVQSSTAGNTLRNFSLGPTRRSLTGSLSGHPSRIHPRQMASGLEGSEWTRTVLNMGPRSELEGMPPPRTSASSVSLLSVLRQQEGSSQSSVYTSATEGRGFPAPEAEAESTSSTGPSNPASLRNELQCDLRRFFLEYDRLQELDQGISGEPSQSHQAQEMLNNNIEPDRPGPSHQQTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPSYAPGQSQSTFEGVPSSSSQLPPPERLESRTSPSSRLPLRRSSNPQEERTVRGVFNQETGHWERIYSQSASNRPENVSQDALNQEMPEEISEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSSSSASTSENAGPSVEGNDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSSSRSTERPGTSRATWRTDRDLGLMNAIGLQPRNPTTSVTSQGTQTLAPQLQNAETQTEREVQEPGSLTSGVTEGGSDYGATGEDALIRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESTGLEGGSAQPSTSQELITELEGRTLSESMQVTERGLSPQTSSSEVEGEANGQSLPEQAHSSMDTEGPVEYSDLTNNNHLPDNTNFYSNESTNGESRNR

Target ORF information:

RefSeq Version	XM_014070575.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014070575.1

ORF Insert Sequence:

ATGAAGGTTG TACCAGAAAA GAATGCTGTG CGAATCCTTT GGGGCCGAGA ACGGGGAACA 
CAAGCTTTGG GAGCACAGCG TCTTTTGCAG GAGTTGGTGG AAGACAAAAC CCGTTGGATG 
AAATGGGAAG GGAAGAAAGT TGAGTTACCG GATAGTCCTC GCTCCACTTT TCTGCTAGCA 
TTCAGTCCAG ACAGGACTCT CATGGCATCA ACTCATGTGA ACCATAATAT TTACATCACA 
GAGGTGAAAA CAGGAAGGTG TGTTCACTCG CTGGTTGGAC ATCGACGCAC ACCGTGGTGT 
GTAACTTTTC ATCCCACTAT CCCAGGGCTT ATTGCTTCAG GGTGCCTCGA TGGAGAAGTT 
AGAATCTGGG ATTTGCATGG TGGAAGTGAG AGCTGGTTTA CAGATAGCAA CAATGCCATT 
GCATCCTTGG CTTTTCATCC AACGGCTCAA CTTCTGCTAA TTGCAACAGC CAATGAGATT 
CACTTCTGGG ACTGGAGTCG CCGGGAGCCT TTTGCAGTTG TGAAAACAGC TAGTGAAATG 
GAACGAGTCC GACTAGTACG ATTTGATCCC CTTGGGCATT ATTTACTTAC AGCAATTGTT 
AACCCTTCCA ACCAGCAGAA TGATGAAGAG GTGGAAATAC CTGTGGATAG TACAGAAATA 
CCACATTATC GTCAACGTTC CATTCTGCAA TCTCAACCAG TGAGACGCAC ACCACTCCTT 
CATAATTTCC TGCATATGCT GTCATCACGC TCTTCTGGTA TCCAGGACAA CGCCCCTTCC 
ACATCCTCTG GGTCCACTGG CACTTCCTTT TCTTCTGTGC AAATGGAACC TTACCAACCC 
CAGGAGCAGG CATCTTCAGC ACAGCAGGAG CAGGGACTCC TTAATCGACC ATCTGCTTTC 
AGCACTGTTC AGAGCAGTAC TGCTGGCAAC ACCCTGCGCA ACTTTAGCCT GGGCCCCACT 
CGTAGATCCC TTACTGGGTC TTTGTCAGGC CATCCGTCCA GGATCCACCC AAGACAGATG 
GCCTCAGGGT TGGAGGGTTC TGAGTGGACT CGAACAGTGT TGAATATGGG CCCTCGCTCT 
GAGTTGGAAG GTATGCCTCC TCCTAGAACC AGTGCCTCTT CAGTGAGTTT GTTGTCGGTG 
CTGAGACAGC AGGAAGGAAG TTCCCAGTCC TCTGTATATA CTTCAGCTAC AGAAGGGAGG 
GGTTTCCCAG CTCCTGAAGC TGAAGCAGAA AGCACCAGTA GTACTGGCCC AAGCAATCCA 
GCCAGCCTTC GCAATGAGCT TCAGTGTGAC TTGAGGCGGT TCTTCTTGGA ATATGATAGG 
CTTCAAGAAC TAGATCAGGG GATAAGTGGT GAACCTAGTC AGTCACATCA GGCCCAGGAA 
ATGCTTAATA ACAACATTGA GCCTGATAGG CCTGGTCCTT CTCATCAACA GACCCCACAT 
AGCAGTGAAA ACAATTCCAA TTTGTCACGG GGGCATCTGA ACCGCTGTCG CGCCTGTCAC 
AACCTGCTGA CTTTTAACAA TGACACACTT CGCTGGGAAA GAAGCACACC CAGCTATGCA 
CCAGGGCAGT CCCAAAGCAC ATTTGAGGGG GTGCCATCCA GCAGTAGCCA GCTACCACCA 
CCTGAGAGAC TGGAAAGCCG AACTTCTCCC TCTAGCAGGC TGCCCCTGAG AAGATCTTCT 
AATCCTCAAG AGGAAAGGAC TGTGAGAGGG GTCTTTAATC AAGAGACGGG GCACTGGGAA 
AGAATTTACA GCCAATCTGC TTCAAATAGA CCTGAGAATG TATCACAGGA CGCCTTAAAC 
CAGGAAATGC CTGAGGAAAT TTCAGAGGAG GATTCACTCA GGAGGAGGCT GTTGGAATCT 
TCACTCATTT CACTCTCACG TTATGATGGG GCAGGATCCC GCGAACATCC TATATATCCT 
GATCCAGCTA GGTTGTCACC TGCTGCTTAT TATGCCCAGA GAATGATCCA GTATCTTTCA 
CGCAGAGATA GCATTCGCCA GCGCTCCATG CGGTATCAGC AAAATCGCCT CCGCTCTTCC 
TCATCTTCTG CTTCCACTTC TGAGAATGCA GGTCCATCTG TAGAAGGAAA TGACCTGGAA 
TTTGAAGACT TCGAGGACAA TGGAGACAGG TCAAGACACC GAGCCCCTAG GAATGCCCGT 
ATGTCTGCAC CATCCCTAGG ACGCTTCGTC CCAAGACGCT TCTTGTTGCC TGAGTATTTG 
CCTTACGCTG GGATTTTCCA TGAGCGTGGA CAGCCTGGGT TGGCCACTCA CTCCTCTGTC 
AATAGAGTTT TAGCAGGGGC TGTAATTGGA GATGGACAGT CTGCTGTGGC CAGCAATATT 
GCAAACACCA CATACCGGCT CCAGTGGTGG GATTTCACTA AATTCGACTT ACCAGAAATA 
AGCAATGCAT CTGTGAATGT GCTTGTTCAG AACTGCAAGA TATACAATGA TGCCAGCTGT 
GATATTTCTG CAGATGGGCA GCTTTTAGCA GCTTTCATTC CTAGCAGTCA GCGAGGATTT 
CCTGATGAGG GGATACTCGC AGTCTATTCC CTTGCCCCAC ACAACTTGGG TGAAATGCTG 
TATACGAAGC GATTTGGTCC CAATGCCATT TCTGTGAGCT TGTCCCCAAT GGGCAGGTAT 
GTCATGGTAG GATTGGCTTC TCGTCGCATC TTGTTGCACC CATCTACAGA ACATATGGTG 
GCTCAGGTTT TCAGGCTTCA GCAACCACAT GGAGGAGAGA CTTCAATGAG GAGAGTGTTC 
AATGTCTTGT ACCCAATGCC TGCCGATCAA AGGAGACACG TCAGCATAAA TTCTGCTCGC 
TGGTTACCTG AGCCGGGTTT AGGATTAGCT TATGGCACTA ATAAAGGGGA TCTGGTGATT 
TGCCGACCAG AGGCGTTAAA CTCTGGTGTA GAATACTACT GGGACCAGCT GAATGAAACT 
GTCTTCACTG TGCATTCCAG CAGCAGAAGC ACTGAACGCC CAGGAACCAG CAGAGCCACC 
TGGAGGACGG ACCGGGATTT GGGCTTAATG AATGCCATTG GCCTGCAGCC ACGGAACCCA 
ACCACCTCTG TGACTTCGCA GGGCACACAG ACTCTGGCAC CTCAGCTACA GAATGCAGAA 
ACACAAACCG AGAGAGAAGT TCAGGAACCA GGAAGTCTTA CTTCAGGGGT TACTGAAGGT 
GGTTCAGATT ATGGAGCAAC TGGAGAAGAT GCCCTAATTA GGATCCAAAG GCTGATGGCT 
GAAGGTGGCA TGACTGCTGT CGTCCAACGA GAGCAAAGCA CTACCATGGC ATCCATGGGT 
GGTTTTGGCA ACAACATAAT TGTGAGTCAC AGGATCCACC GTAGCTCTCA GACTGGCACT 
GAATCCACTG GCTTAGAAGG AGGCTCAGCT CAACCATCGA CTTCGCAGGA GCTGATAACA 
GAGCTAGAAG GACGGACCCT TTCTGAGTCT ATGCAGGTGA CAGAACGTGG CCTGAGCCCA 
CAGACATCGT CTAGTGAGGT TGAGGGAGAA GCTAATGGGC AAAGCCTACC AGAGCAAGCT 
CATTCCTCAA TGGACACTGA GGGACCAGTC GAGTATAGTG ATCTAACTAA TAATAACCAT 
CTTCCTGACA ATACCAATTT CTATAGTAAT GAGAGCACCA ATGGAGAGTC TCGGAACAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

AMBRA1 cDNA ORF clone, Thamnophis sirtalis

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム