DACT1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	DACT1
Entrez Gene ID	106550598
Full Name	dishevelled binding antagonist of beta catenin 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014068534.1	XP_013924009.1	dapper homolog 1

Pathways from BioSystems
Gene Ontology

The following DACT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DACT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh26219	XM_014068534.1 Latest version!	Thamnophis sirtalis dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DACT1 ( XM_014068534.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013924009.1 Thamnophis sirtalis dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.

Price & Availability↑

CloneID	OTh26219
Clone ID Related Accession (Same CDS sequence)	XM_014068534.1
Accession Version	XM_014068534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	dapper homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013659219.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GTCCGGAGCC AGGGAAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGGC 
CAGGCGACGG AGGCGGAGCG GCTGCGCACC CGGGAGCGGC TGGAGGCCAC ACTCGCCGGG 
CTGGCCGAAT TGGAATTCCT GCGCCAGCGC CAGGAGCTGC GCGTCAAGCG GTTACTGGCC 
GCCGCGCCCT CGGCTTCTCT GGCCGGGATC TGCTCCGGGG AAACGACCAG AGCCCCCGCA 
GGAGCCGGGA TCCCGCCGCG CAGCTTAGAA GAGAAGTTCC TGGAGGAGAA CATCCTGCTT 
CTGCGGAGAC AGCTGAACTG CTTAAGAAGA AGAGATGCTG GCTTGTTAAA CCAGTTGCAA 
GAACTGGACA AACAGATCAG TGATTTGAGG CTGGATGTTG AAAAGACAAC AGATGAACAC 
CTTGACATGG ATAGCCGTCC CAGTTCAGGG TTTTATGAGC TGAGTGATGG GGCTTCTGGA 
TCTCTCTCCA ATTCGTCCAA CTCTGTTTTC AGTGAATGTT TATCCAGTTG TCACTCCAGT 
ACTTGCTTCT GCAGCTCTTT GGATGCATCC TTGAGTGGCT CTGATGGACG GCCCAAGTCT 
GCAGATTTCA TCAGCTGGCT GGAGCAAAAC AAAGGAATGC AACAAAATGA GCAAGCGGGG 
ATGGCCTCTC CCTCAATGCC ACACCCAGAT TCTTTTGACA TCGTTGCAGA TGTACATCCA 
AAATACCAGT GTGATCTGGT GTCTAAAACC GGAAATGATG TGTACCGTTA TCCCAGCCCT 
TTACATGCTG TTGCAGTGCA GAGCCCCATG TTTCTTCTAC CGGTGACCAG TTCCCCCCCA 
AGAGAAGAAG CCGAGAGAAC CAGTGACAGT GCTGTAGATG TTGGACCGGA ACTCAACTCC 
GAAAAAACGG TGGATTCTTG TCTTTCACAC ACTTCTCGTT CTCCATCCTG TCCTTTGCCC 
AGCAGAAAGA TGGATGGATA TATTTTAAGC CTTGTGCAAA AAAAGGCTCC TTTGGTGAGG 
ACTAATAAAC CAAGAACAAA TCTGAATGCA GACCTGGCCA AGGGGATTTT GAGACATGGA 
AGCTTGTGTC TTCGGCAGAT GGCTACTGCC GCGCCTAGCA ATACCTCTAA CACGAAGAAT 
CTCATCCAGC CACTTTCACA TTCCAGTGGA ACCATTCTCA CCCTAGAAAA CAGTGCCTTT 
TCTCCCATAA AGCAGAATTC AAAGGAAGTA GAGAATACGG AGTTAAAAAA GATACCCTCA 
CCTGCTGTTT TCCCACCGAA TGCTAATAAT GATCAAAGCA AGCCCTCAGT GAAGAATGTC 
AAAACTCAAG AAGTGATGCG ATCCCCTGTG ACTAAGAGTG ATGCTCCCAA GGAAAGTGGC 
CAATTCTATT TGATCTGTCC TAAAGAAAGC CCAGAACAGC ACATAACACC CTTGAAAGAA 
AAGAAAGCCG TCCAGCTTCC CAAAAAGCTA TCGTTTAAAA GTAGTAGTGG GATGCAGTCG 
GCTTGCTCTG TTTTATCAGG CCAGGAGAGC AGACCATTGG TGGAACTTAA GAGTGAGGGG 
TCCTCATCCC AAAGTCTGGA GGAGGGATTC CTAGTGAATG CCCAGTTCAT TCCTGCTCAG 
CCACAAACAG TCAGACTTCT CAAAGGCACC AGGAGTGTCA AAATTCTCAA AGGTTCTATG 
GTGAAGCACA GATCTCATCT GGTCACAGTG CTGGAAAATG GACCTCAGGC TGGACGGGAG 
AGGGCCAAAC CAACAGGCAA AAAGTGTCAC TTCCTGGATG AATTGGATCC AAACAAGAAG 
CCAAGAAAGA CCACTTTGAG AGGGAAGAAA AGTCTCCACA TTCAGCCAGA TGCCAACCAT 
CCGACTCGGC AGCCAGGCGT ACTCAAGTCT GTGCTGAGGC CCCATGGACA TAGCAGAGAA 
CCAGTAGTTG CCAAGCCCAA GCACAAGCGA GCGGATTACC GGCGGTGGAA ACTCTCTACA 
GAGGTATCTT ACGAAGATGC CCTCAGGCGG GCCAGGAGGA ACAGGAGAGA AGCTATAGGC 
GTCTATTCTG AGATTCCACA CCCTTATATT AGCCCCTATG CCTATGTTGC TAGTGACTCA 
GAATATTCGG CTGAATGTGA ATCCCTCTTC CACTCCACTG TAGTGGACAC CAGCGAGGAT 
GAAAGGAGCA ACTATACCAC CAACTGCTTT GGGGACAGCG AGTCCAGCTT AAGTGGAGTG 
GAGTTTGTTG GTGAAAGCAC AACAACCAGT GACTCAGAGG AAAGCGGGGG TCTCCTTTGG 
TCCCAGTTTG TTCAGACACT TCCCATCCAG GCAGTGGCTG CTGCTTCGGA GCTCCATGAA 
AATGCAGCCA AGGCCTTTGT TAAGATTAAA GCATCCCATA ACCTTAAGAA GAAAATCCTT 
CGCTTTCGGT CCGGCTCTCT CAAACTGATG ACTACTGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013924009.1
CDS	1..2442
Translation	MKASPEPGKQQQQQQQQQQGQATEAERLRTRERLEATLAGLAELEFLRQRQELRVKRLLAAAPSASLAGICSGETTRAPAGAGIPPRSLEEKFLEENILLLRRQLNCLRRRDAGLLNQLQELDKQISDLRLDVEKTTDEHLDMDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSSLDASLSGSDGRPKSADFISWLEQNKGMQQNEQAGMASPSMPHPDSFDIVADVHPKYQCDLVSKTGNDVYRYPSPLHAVAVQSPMFLLPVTSSPPREEAERTSDSAVDVGPELNSEKTVDSCLSHTSRSPSCPLPSRKMDGYILSLVQKKAPLVRTNKPRTNLNADLAKGILRHGSLCLRQMATAAPSNTSNTKNLIQPLSHSSGTILTLENSAFSPIKQNSKEVENTELKKIPSPAVFPPNANNDQSKPSVKNVKTQEVMRSPVTKSDAPKESGQFYLICPKESPEQHITPLKEKKAVQLPKKLSFKSSSGMQSACSVLSGQESRPLVELKSEGSSSQSLEEGFLVNAQFIPAQPQTVRLLKGTRSVKILKGSMVKHRSHLVTVLENGPQAGRERAKPTGKKCHFLDELDPNKKPRKTTLRGKKSLHIQPDANHPTRQPGVLKSVLRPHGHSREPVVAKPKHKRADYRRWKLSTEVSYEDALRRARRNRREAIGVYSEIPHPYISPYAYVASDSEYSAECESLFHSTVVDTSEDERSNYTTNCFGDSESSLSGVEFVGESTTTSDSEESGGLLWSQFVQTLPIQAVAAASELHENAAKAFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	XM_014068534.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014068534.1

ORF Insert Sequence:

ATGAAGGCGA GTCCGGAGCC AGGGAAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGGC 
CAGGCGACGG AGGCGGAGCG GCTGCGCACC CGGGAGCGGC TGGAGGCCAC ACTCGCCGGG 
CTGGCCGAAT TGGAATTCCT GCGCCAGCGC CAGGAGCTGC GCGTCAAGCG GTTACTGGCC 
GCCGCGCCCT CGGCTTCTCT GGCCGGGATC TGCTCCGGGG AAACGACCAG AGCCCCCGCA 
GGAGCCGGGA TCCCGCCGCG CAGCTTAGAA GAGAAGTTCC TGGAGGAGAA CATCCTGCTT 
CTGCGGAGAC AGCTGAACTG CTTAAGAAGA AGAGATGCTG GCTTGTTAAA CCAGTTGCAA 
GAACTGGACA AACAGATCAG TGATTTGAGG CTGGATGTTG AAAAGACAAC AGATGAACAC 
CTTGACATGG ATAGCCGTCC CAGTTCAGGG TTTTATGAGC TGAGTGATGG GGCTTCTGGA 
TCTCTCTCCA ATTCGTCCAA CTCTGTTTTC AGTGAATGTT TATCCAGTTG TCACTCCAGT 
ACTTGCTTCT GCAGCTCTTT GGATGCATCC TTGAGTGGCT CTGATGGACG GCCCAAGTCT 
GCAGATTTCA TCAGCTGGCT GGAGCAAAAC AAAGGAATGC AACAAAATGA GCAAGCGGGG 
ATGGCCTCTC CCTCAATGCC ACACCCAGAT TCTTTTGACA TCGTTGCAGA TGTACATCCA 
AAATACCAGT GTGATCTGGT GTCTAAAACC GGAAATGATG TGTACCGTTA TCCCAGCCCT 
TTACATGCTG TTGCAGTGCA GAGCCCCATG TTTCTTCTAC CGGTGACCAG TTCCCCCCCA 
AGAGAAGAAG CCGAGAGAAC CAGTGACAGT GCTGTAGATG TTGGACCGGA ACTCAACTCC 
GAAAAAACGG TGGATTCTTG TCTTTCACAC ACTTCTCGTT CTCCATCCTG TCCTTTGCCC 
AGCAGAAAGA TGGATGGATA TATTTTAAGC CTTGTGCAAA AAAAGGCTCC TTTGGTGAGG 
ACTAATAAAC CAAGAACAAA TCTGAATGCA GACCTGGCCA AGGGGATTTT GAGACATGGA 
AGCTTGTGTC TTCGGCAGAT GGCTACTGCC GCGCCTAGCA ATACCTCTAA CACGAAGAAT 
CTCATCCAGC CACTTTCACA TTCCAGTGGA ACCATTCTCA CCCTAGAAAA CAGTGCCTTT 
TCTCCCATAA AGCAGAATTC AAAGGAAGTA GAGAATACGG AGTTAAAAAA GATACCCTCA 
CCTGCTGTTT TCCCACCGAA TGCTAATAAT GATCAAAGCA AGCCCTCAGT GAAGAATGTC 
AAAACTCAAG AAGTGATGCG ATCCCCTGTG ACTAAGAGTG ATGCTCCCAA GGAAAGTGGC 
CAATTCTATT TGATCTGTCC TAAAGAAAGC CCAGAACAGC ACATAACACC CTTGAAAGAA 
AAGAAAGCCG TCCAGCTTCC CAAAAAGCTA TCGTTTAAAA GTAGTAGTGG GATGCAGTCG 
GCTTGCTCTG TTTTATCAGG CCAGGAGAGC AGACCATTGG TGGAACTTAA GAGTGAGGGG 
TCCTCATCCC AAAGTCTGGA GGAGGGATTC CTAGTGAATG CCCAGTTCAT TCCTGCTCAG 
CCACAAACAG TCAGACTTCT CAAAGGCACC AGGAGTGTCA AAATTCTCAA AGGTTCTATG 
GTGAAGCACA GATCTCATCT GGTCACAGTG CTGGAAAATG GACCTCAGGC TGGACGGGAG 
AGGGCCAAAC CAACAGGCAA AAAGTGTCAC TTCCTGGATG AATTGGATCC AAACAAGAAG 
CCAAGAAAGA CCACTTTGAG AGGGAAGAAA AGTCTCCACA TTCAGCCAGA TGCCAACCAT 
CCGACTCGGC AGCCAGGCGT ACTCAAGTCT GTGCTGAGGC CCCATGGACA TAGCAGAGAA 
CCAGTAGTTG CCAAGCCCAA GCACAAGCGA GCGGATTACC GGCGGTGGAA ACTCTCTACA 
GAGGTATCTT ACGAAGATGC CCTCAGGCGG GCCAGGAGGA ACAGGAGAGA AGCTATAGGC 
GTCTATTCTG AGATTCCACA CCCTTATATT AGCCCCTATG CCTATGTTGC TAGTGACTCA 
GAATATTCGG CTGAATGTGA ATCCCTCTTC CACTCCACTG TAGTGGACAC CAGCGAGGAT 
GAAAGGAGCA ACTATACCAC CAACTGCTTT GGGGACAGCG AGTCCAGCTT AAGTGGAGTG 
GAGTTTGTTG GTGAAAGCAC AACAACCAGT GACTCAGAGG AAAGCGGGGG TCTCCTTTGG 
TCCCAGTTTG TTCAGACACT TCCCATCCAG GCAGTGGCTG CTGCTTCGGA GCTCCATGAA 
AATGCAGCCA AGGCCTTTGT TAAGATTAAA GCATCCCATA ACCTTAAGAA GAAAATCCTT 
CGCTTTCGGT CCGGCTCTCT CAAACTGATG ACTACTGTCT AA