NOP2 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	NOP2
Entrez Gene ID	106549846
Full Name	NOP2 nucleolar protein
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014067599.1	XP_013923074.1	probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase

Pathways from BioSystems
Gene Ontology

The following NOP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh25509	XM_014067599.1 Latest version!	Thamnophis sirtalis NOP2 nucleolar protein (NOP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOP2 ( XM_014067599.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013923074.1 Thamnophis sirtalis NOP2 nucleolar protein (NOP2), mRNA.

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CloneID	OTh25509
Clone ID Related Accession (Same CDS sequence)	XM_014067599.1
Accession Version	XM_014067599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013659078.1) and transcript sequence (GDKU01070205.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 74 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGGCGCA AGTTGGACCC GACCCGGCAG GTAAAGCGGG GGCCAGGTCG GAAGGCCCGC 
AAGCAGCGTG GGGCGGAGGA GGAGCTCGCC CGCTTTTTGC CGCCCGCACT TGATGACAAT 
GCAGCCAAAC TTTCCAGTCA TGCCCGACGA AGAGCTGCAA AAAGACGGGT TAAATCTGAG 
CAACTGCAGA AGAACAAGAA TGCCCCCAAT CACCAAAATG TGACAGCTAA AAAGAAAACA 
CTCAAGAATG CAAAGAAGGG GAAAAGTTCC CCAGAGGACA ACCCAACTAA CGGCAGCACT 
GATGCGGCCA TGCAGCTCCC TTCCCCTGCC TTAAGGAAGA AAAGGTCCAG GACCGAAGAG 
AGTGAAGAAG ACAAAGAAGA GAGCATGGCT TTCAGTGATG AAGATGATCT AAGTGAGGAT 
GAGATTAGGG ATGACTATGG AGTTGCTTCC TCTAGTGATG AAGAGCTTCT GCCCATAGAA 
CGAGCTGCTC GAAAACAAAA GATGGAAGGG CAGGACATCA GTGAGGATGA CAGTGAGGAT 
GAGACACCAC CATCCAGCCA GAGGATAGAG CAAAAGGAAG AAAAGGATGC AGACCTGCAG 
CTCAACATGG AGGCAGAGGA GTGCTTCGTG CTTCCCAGTG GGCAGGAGAT AGAGAAAGAA 
ACTGCTCAGC CTCCTGATCT CCAACTCATT CATCAGCGTA TAAAAGATAA CATGGAGGTG 
CTTCAGAATT TCAGTTCAAA GCGAGAGGAA GGACGCTCAA GGCAGGAATA TTTATCCCTT 
CTGCATCAGG ATCTTGCCGC CTATTACTCT TACAGTGATT TCTTAATTGG AAAGATGACA 
GAACTCTTCC CTCTGTCTGA GCTGGTTGAA TTCTTGGAAG CCAATGAGGT CTCTCGACCA 
GTGACCCTCC GCACCAATAC ACTGAAGACA AGACGCCGTG ATTTAGCCCA GGCCTTGATA 
AATCGTGGAG TGAACTTAGA CCCTTTAGGA AAGTGGTCAA AAACTGGGCT TGTTGTTTAT 
GACTCCTCTG TACCTATAGG TGCCACCCCA GAATACTTAG CTGGACACTA CATGCTGCAA 
GGTGCTTCCA GCCTTCTCCC TGTTATGGCT CTGGCCCCTC AGGAGAACGA GCGCATTCTG 
GACATGTGCT GTGCCCCAGG AGGCAAGACC AGTTACATAG CCCAGCTGAT GAAGAACACA 
GGGGTGATAC TAGCTAATGA CAGCAATTCG GAGCGTTTGC ACAGTGTGGT GGGAAACTTG 
CACCGCTTAG GAGTCACAAA CACTGTTGTG AGCAACTGTG ATGGGCGCCA GTTTCCTAAG 
GTACTGGGTG GATTTGACAG AGTCCTGCTT GATGCTCCCT GCAGTGGTAC TGGTGTTATT 
TCAAAGGACC CCACAGTCAA AACTAATAAG GATGAAAAGG ACATTTTGCG CTGTGCTCAC 
CTACAGAAGG AGTTGATCCT CAGTGCCATT GACTCCGTTA ACGCAGCCTC TGAGACCGGG 
GGATACGTTG TGTACTGCAC TTGTTCAGTC ATGGTGGAAG AGAACGAGTG GGTGGTGGAT 
TATGCCCTGA AGAAGCGCAA CGTCCGCTTG GTGCCAACGG GTCTGGACTT TGGCAAAGAA 
GGATTTACCC GGTTTAGGGA GCGTCGTTTC CATCCGTCCT TGAAATCCAC GCGCCGTTTC 
TATCCTCACA CCCACAATAT GGATGGTTTT TTTGTGGCCA AATTGAAAAA ATTTTCCAAT 
GCCATTCCTA AACCTCAGAA AGATGAAGTG CCCAGTGAGG AGCTAACAAT GCAAACAGTA 
AAAAATGGAT CTGCTCCAAC TGAACCTCCA CCTAAAAAGA AAAAAGATGC AGAAAGTAAA 
GCCAGTCCGC AGCAGGCTTC AAAGCAAGAG CTTTCAGCTG CAGTCGCAGC CAGGAAGGAG 
GCTAAAGGGG AGTTGAAAGC TAGTAAAAAG AAGAAAGCGC TTGCTGCCTC CAAACAAAGC 
AACTTTCAGA AAAAGTCCAG ACTCTTGAAT GCTGCTTGGC CCTCTCCTGG AGGACTAAGG 
GCTAAGTCAA CTCCCAGAAA AAAGGGCAGG GTTCAACGAA ACCCACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013923074.1
CDS	59..2149
Translation	MGRKLDPTRQVKRGPGRKARKQRGAEEELARFLPPALDDNAAKLSSHARRRAAKRRVKSEQLQKNKNAPNHQNVTAKKKTLKNAKKGKSSPEDNPTNGSTDAAMQLPSPALRKKRSRTEESEEDKEESMAFSDEDDLSEDEIRDDYGVASSSDEELLPIERAARKQKMEGQDISEDDSEDETPPSSQRIEQKEEKDADLQLNMEAEECFVLPSGQEIEKETAQPPDLQLIHQRIKDNMEVLQNFSSKREEGRSRQEYLSLLHQDLAAYYSYSDFLIGKMTELFPLSELVEFLEANEVSRPVTLRTNTLKTRRRDLAQALINRGVNLDPLGKWSKTGLVVYDSSVPIGATPEYLAGHYMLQGASSLLPVMALAPQENERILDMCCAPGGKTSYIAQLMKNTGVILANDSNSERLHSVVGNLHRLGVTNTVVSNCDGRQFPKVLGGFDRVLLDAPCSGTGVISKDPTVKTNKDEKDILRCAHLQKELILSAIDSVNAASETGGYVVYCTCSVMVEENEWVVDYALKKRNVRLVPTGLDFGKEGFTRFRERRFHPSLKSTRRFYPHTHNMDGFFVAKLKKFSNAIPKPQKDEVPSEELTMQTVKNGSAPTEPPPKKKKDAESKASPQQASKQELSAAVAARKEAKGELKASKKKKALAASKQSNFQKKSRLLNAAWPSPGGLRAKSTPRKKGRVQRNPQ

Target ORF information:

RefSeq Version	XM_014067599.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis NOP2 nucleolar protein (NOP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014067599.1

ORF Insert Sequence:

ATGGGGCGCA AGTTGGACCC GACCCGGCAG GTAAAGCGGG GGCCAGGTCG GAAGGCCCGC 
AAGCAGCGTG GGGCGGAGGA GGAGCTCGCC CGCTTTTTGC CGCCCGCACT TGATGACAAT 
GCAGCCAAAC TTTCCAGTCA TGCCCGACGA AGAGCTGCAA AAAGACGGGT TAAATCTGAG 
CAACTGCAGA AGAACAAGAA TGCCCCCAAT CACCAAAATG TGACAGCTAA AAAGAAAACA 
CTCAAGAATG CAAAGAAGGG GAAAAGTTCC CCAGAGGACA ACCCAACTAA CGGCAGCACT 
GATGCGGCCA TGCAGCTCCC TTCCCCTGCC TTAAGGAAGA AAAGGTCCAG GACCGAAGAG 
AGTGAAGAAG ACAAAGAAGA GAGCATGGCT TTCAGTGATG AAGATGATCT AAGTGAGGAT 
GAGATTAGGG ATGACTATGG AGTTGCTTCC TCTAGTGATG AAGAGCTTCT GCCCATAGAA 
CGAGCTGCTC GAAAACAAAA GATGGAAGGG CAGGACATCA GTGAGGATGA CAGTGAGGAT 
GAGACACCAC CATCCAGCCA GAGGATAGAG CAAAAGGAAG AAAAGGATGC AGACCTGCAG 
CTCAACATGG AGGCAGAGGA GTGCTTCGTG CTTCCCAGTG GGCAGGAGAT AGAGAAAGAA 
ACTGCTCAGC CTCCTGATCT CCAACTCATT CATCAGCGTA TAAAAGATAA CATGGAGGTG 
CTTCAGAATT TCAGTTCAAA GCGAGAGGAA GGACGCTCAA GGCAGGAATA TTTATCCCTT 
CTGCATCAGG ATCTTGCCGC CTATTACTCT TACAGTGATT TCTTAATTGG AAAGATGACA 
GAACTCTTCC CTCTGTCTGA GCTGGTTGAA TTCTTGGAAG CCAATGAGGT CTCTCGACCA 
GTGACCCTCC GCACCAATAC ACTGAAGACA AGACGCCGTG ATTTAGCCCA GGCCTTGATA 
AATCGTGGAG TGAACTTAGA CCCTTTAGGA AAGTGGTCAA AAACTGGGCT TGTTGTTTAT 
GACTCCTCTG TACCTATAGG TGCCACCCCA GAATACTTAG CTGGACACTA CATGCTGCAA 
GGTGCTTCCA GCCTTCTCCC TGTTATGGCT CTGGCCCCTC AGGAGAACGA GCGCATTCTG 
GACATGTGCT GTGCCCCAGG AGGCAAGACC AGTTACATAG CCCAGCTGAT GAAGAACACA 
GGGGTGATAC TAGCTAATGA CAGCAATTCG GAGCGTTTGC ACAGTGTGGT GGGAAACTTG 
CACCGCTTAG GAGTCACAAA CACTGTTGTG AGCAACTGTG ATGGGCGCCA GTTTCCTAAG 
GTACTGGGTG GATTTGACAG AGTCCTGCTT GATGCTCCCT GCAGTGGTAC TGGTGTTATT 
TCAAAGGACC CCACAGTCAA AACTAATAAG GATGAAAAGG ACATTTTGCG CTGTGCTCAC 
CTACAGAAGG AGTTGATCCT CAGTGCCATT GACTCCGTTA ACGCAGCCTC TGAGACCGGG 
GGATACGTTG TGTACTGCAC TTGTTCAGTC ATGGTGGAAG AGAACGAGTG GGTGGTGGAT 
TATGCCCTGA AGAAGCGCAA CGTCCGCTTG GTGCCAACGG GTCTGGACTT TGGCAAAGAA 
GGATTTACCC GGTTTAGGGA GCGTCGTTTC CATCCGTCCT TGAAATCCAC GCGCCGTTTC 
TATCCTCACA CCCACAATAT GGATGGTTTT TTTGTGGCCA AATTGAAAAA ATTTTCCAAT 
GCCATTCCTA AACCTCAGAA AGATGAAGTG CCCAGTGAGG AGCTAACAAT GCAAACAGTA 
AAAAATGGAT CTGCTCCAAC TGAACCTCCA CCTAAAAAGA AAAAAGATGC AGAAAGTAAA 
GCCAGTCCGC AGCAGGCTTC AAAGCAAGAG CTTTCAGCTG CAGTCGCAGC CAGGAAGGAG 
GCTAAAGGGG AGTTGAAAGC TAGTAAAAAG AAGAAAGCGC TTGCTGCCTC CAAACAAAGC 
AACTTTCAGA AAAAGTCCAG ACTCTTGAAT GCTGCTTGGC CCTCTCCTGG AGGACTAAGG 
GCTAAGTCAA CTCCCAGAAA AAAGGGCAGG GTTCAACGAA ACCCACAGTA G